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  1 / 11 MEDLINE  
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[PMID]:27138064
[Au] Autor:Suebsing R; Pradeep PJ; Jitrakorn S; Sirithammajak S; Kampeera J; Turner WA; Saksmerprome V; Withyachumnarnkul B; Kiatpathomchai W
[Ad] Endereço:Bioengineering and Sensing Technology Laboratory, National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand.
[Ti] Título:Detection of natural infection of infectious spleen and kidney necrosis virus in farmed tilapia by hydroxynapthol blue-loop-mediated isothermal amplification assay.
[So] Source:J Appl Microbiol;121(1):55-67, 2016 Jul.
[Is] ISSN:1365-2672
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: Infectious spleen and kidney necrosis virus (ISKNV) has recently been recognized as a causative agent of serious systemic disease in tilapia. Our objective was to establish a new colorimetric loop-mediated isothermal amplification (LAMP) assay with pre-addition of hydroxynapthol blue (blue-LAMP) to investigate ISKNV transmission in tilapia. METHODS AND RESULTS: The blue-LAMP, targeting a major capsid protein gene of ISKNV, was conducted at 65°C for 45 min, allowing unaided visual detection of the pathogen based on colour change without cross-amplification of other known fish pathogens tested. Comparison of blue-LAMP and PCR assays revealed a higher detection level for blue-LAMP assay (41·33%) in a population of farmed tilapia infected with ISKNV. The investigation of ISKNV transmission pattern in farmed red tilapia using the blue-LAMP revealed a possible matroclinical form. The presence of ISKNV in the gonad samples was confirmed by in situ LAMP assay. Positive signals only appeared in ovarian follicles, and not in oocytes. Moreover, tissue tropism assay revealed that the brain was the main target organ in both farmed red tilapia (40%) and Nile tilapia (20%). CONCLUSIONS: The developed blue-LAMP assay has the potential to be used as a viable tool for screening covert and natural infections of ISKNV in tilapia. The evidence of vertical transmission of ISKNV infection in tilapia indicates the seriousness of this disease and will require a close attention and collaboration between tilapia hatcheries and disease experts in order to find a solution. SIGNIFICANCE AND IMPACT OF THE STUDY: The new blue-LAMP assay is a time-saving and economically viable detection tool, which allows unaided visual detection for ISKNV in tilapia, and it could be applicable for field applications. Evidence on the vertical transmission of ISKNV in farmed tilapia suggests a need for developing farm management practices to control the spread of virus in aquaculture industries.
[Mh] Termos MeSH primário: Doenças dos Peixes/virologia
Técnicas de Amplificação de Ácido Nucleico
Infecções por Retroviridae/veterinária
Tilápia/virologia
[Mh] Termos MeSH secundário: Animais
Aquicultura/métodos
Colorimetria/métodos
Doenças dos Peixes/transmissão
Infecções por Retroviridae/diagnóstico
Infecções por Retroviridae/transmissão
Infecções por Retroviridae/virologia
Sensibilidade e Especificidade
Vírus do Infarto Esplênico do Pato de Trager/genética
Vírus do Infarto Esplênico do Pato de Trager/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160504
[St] Status:MEDLINE
[do] DOI:10.1111/jam.13165


  2 / 11 MEDLINE  
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[PMID]:23763499
[Au] Autor:Tanaka N; Izawa T; Kuwamura M; Higashiguchi N; Kezuka C; Kurata O; Wada S; Yamate J
[Ad] Endereço:Laboratory of Veterinary Pathology, Osaka Prefecture University, Osaka, Japan.
[Ti] Título:The first case of infectious spleen and kidney necrosis virus (ISKNV) infection in aquarium-maintained mandarin fish, Siniperca chuatsi (Basilewsky), in Japan.
[So] Source:J Fish Dis;37(4):401-5, 2014 Apr.
[Is] ISSN:1365-2761
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Animais de Zoológico
Doenças dos Peixes/virologia
Perciformes
Infecções por Retroviridae/veterinária
Vírus do Infarto Esplênico do Pato de Trager/isolamento & purificação
Infecções Tumorais por Vírus/veterinária
[Mh] Termos MeSH secundário: Animais
Doenças dos Peixes/diagnóstico
Doenças dos Peixes/mortalidade
Japão
Dados de Sequência Molecular
Infecções por Retroviridae/diagnóstico
Infecções por Retroviridae/mortalidade
Infecções por Retroviridae/virologia
Análise de Sequência de DNA/veterinária
Vírus do Infarto Esplênico do Pato de Trager/genética
Infecções Tumorais por Vírus/diagnóstico
Infecções Tumorais por Vírus/mortalidade
Infecções Tumorais por Vírus/virologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1412
[Cu] Atualização por classe:140328
[Lr] Data última revisão:
140328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130615
[St] Status:MEDLINE
[do] DOI:10.1111/jfd.12134


  3 / 11 MEDLINE  
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[PMID]:23010220
[Au] Autor:Huang Z; Tang J; Li M; Fu Y; Dong C; Zhong JF; He J
[Ad] Endereço:School of Marine Sciences, Sun Yat-sen University, Guangzhou 510275, PR China. lsshzhj@mail.sysu.edu.cn
[Ti] Título:Immunological evaluation of Vibrio alginolyticus, Vibrio harveyi, Vibrio vulnificus and infectious spleen and kidney necrosis virus (ISKNV) combined-vaccine efficacy in Epinephelus coioides.
[So] Source:Vet Immunol Immunopathol;150(1-2):61-8, 2012 Nov 15.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Combined vaccines are immunological products intended for immunization against multifactorial infectious diseases caused by different types or variants of pathogens. In this study, the effectiveness of Vibrio alginolyticus (Va), Vibrio harveyi (Vh), Vibrio vulnificus (Vv) and infectious spleen and kidney necrosis virus (ISKNV), an iridovirus, combined-vaccine (Vibrio and ISKNV combined vaccines, VICV), Va+Vh+Vv inactive vaccine (VIV) and ISKNV whole cell inactive vaccine (IWCIV) in Epinephelus coioides were evaluated using various immunological parameters including antibody titer, serum lysozyme activity (LA), respiratory burst (RB) activity, bactericidal activity (BA) and relative percentage survival (RPS). E. coioides immunized with VICV and challenged with Va+Vh+Vv+ISKNV had an RPS of 80%. The RPS was 73.3% in E. coioides immunized with VIV and challenged with Va+Vh+Vv. E. coioides immunized with IWCIV and challenged with ISKNV had an RPS of 69.6%. Serum LA in the vaccinated group was significantly higher than the control group on days 21 and 28 post-vaccination (P<0.01). The RB activity of head kidney cells in the vaccinated group was significantly higher (P<0.01) compared to that in the control group. However, RB activity of spleen cells in the vaccinated group and the control group were not significantly different (P>0.05). After immunization with VICV, BA values of blood leucocytes and head kidney cells increased significantly more than spleen cells. BA value of blood leucocytes was higher than that in head kidney cells. There were distinct difference between BA values in head kidney cells and in spleen cells (P<0.05) as well as between BA value of blood leucocytes and head kidney cells (P<0.01). E. coioides vaccinated with VICV have significantly higher antibody levels than control groupers (P<0.01). Our study suggests that the VICV candidate can effectively protect groupers against multiple bacterial and viral pathogens.
[Mh] Termos MeSH primário: Vacinas Bacterianas/farmacologia
Doenças dos Peixes/microbiologia
Perciformes
Vírus do Infarto Esplênico do Pato de Trager/imunologia
Vibrioses/veterinária
Vibrio/imunologia
Vacinas Virais/farmacologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Anticorpos Antivirais/sangue
Aquicultura/métodos
Vacinas Bacterianas/imunologia
Ensaio de Imunoadsorção Enzimática
Doenças dos Peixes/sangue
Doenças dos Peixes/imunologia
Doenças dos Peixes/prevenção & controle
Imunização/veterinária
Muramidase/sangue
Distribuição Aleatória
Explosão Respiratória/imunologia
Análise de Sobrevida
Vacinas Combinadas/imunologia
Vacinas Combinadas/farmacologia
Vibrioses/imunologia
Vibrioses/microbiologia
Vibrioses/prevenção & controle
Vacinas Virais/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Bacterial); 0 (Antibodies, Viral); 0 (Bacterial Vaccines); 0 (Vaccines, Combined); 0 (Viral Vaccines); EC 3.2.1.17 (Muramidase)
[Em] Mês de entrada:1306
[Cu] Atualização por classe:121012
[Lr] Data última revisão:
121012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120927
[St] Status:MEDLINE


  4 / 11 MEDLINE  
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[PMID]:22844427
[Au] Autor:Guo CJ; Yang LS; Zhang YF; Wu YY; Weng SP; Yu XQ; He JG
[Ad] Endereço:MOE Key Laboratory of Aquatic Product Safety, School of Marine Science, Sun Yat-sen University, Guangzhou, PR China.
[Ti] Título:A novel viral SOCS from infectious spleen and kidney necrosis virus: interacts with Jak1 and inhibits IFN-α induced Stat1/3 activation.
[So] Source:PLoS One;7(7):e41092, 2012.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Interferon (IFN)-induced Janus kinase (Jak)/signal transducer and activator of transcription (Stat) pathway is important in controlling immune responses and is negatively response-regulated by the suppressor of cytokine signaling (SOCS) proteins. However, several viruses have developed various strategies to inhibit this pathway to circumvent the anti-viral immunity of the host. The infectious spleen and kidney necrosis virus (ISKNV) is the type species of the genus Megalocytivirus in the family Iridoviridae and a causative agent of epizootics in fish. ISKNV ORF103R encodes a predicted viral SOCS (vSOCS) with high homology to the vertebrate SOCS1, but lacks a SOCS-box domain. Interestingly, vSOCS only exists in the genus Megalocytivirus. ISKNV-vSOCS can block the IFN-α-induced Jak/Stat pathway in HepG2 cells. Over-expression of ISKNV-vSOCS inhibited the activities of IFN-stimulated response element (ISRE) promoter; however, the inhibitions by ISKNV-vSOCS were dose-dependent. ISKNV-vSOCS interacted with Jak1 protein and inhibited its tyrosine kinase activity in vitro. ISKNV-vSOCS also impaired the phosphorylation of Stat1 and Stat3 proteins and suppressed their activations. The point mutations (F18D, S66A, S85A, and R64K) of ISKNV-vSOCS significantly impaired the inhibition of IFN-α-induced ISRE-promoter activation. In conclusion, vSOCS inhibits IFN-α-induced Stat1/Stat3 signaling, suggesting that Megalocytivirus has developed a novel strategy to evade IFN anti-viral immunity via vSOCS protein.
[Mh] Termos MeSH primário: Interferon-alfa/farmacologia
Janus Quinase 1/metabolismo
Rim/virologia
Fatores de Transcrição STAT/metabolismo
Proteínas Supressoras da Sinalização de Citocinas/metabolismo
Vírus do Infarto Esplênico do Pato de Trager
Proteínas Virais/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Biologia Computacional
Genes Reporter/genética
Células Hep G2
Seres Humanos
Camundongos
Dados de Sequência Molecular
Fosforilação/efeitos dos fármacos
Mutação Puntual
Ligação Proteica
Fator de Transcrição STAT1/metabolismo
Fator de Transcrição STAT3/metabolismo
Transdução de Sinais/efeitos dos fármacos
Proteínas Supressoras da Sinalização de Citocinas/química
Proteínas Supressoras da Sinalização de Citocinas/genética
Proteínas Virais/química
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Interferon-alpha); 0 (STAT Transcription Factors); 0 (STAT1 Transcription Factor); 0 (STAT3 Transcription Factor); 0 (Suppressor of Cytokine Signaling Proteins); 0 (Viral Proteins); EC 2.7.10.2 (Janus Kinase 1)
[Em] Mês de entrada:1211
[Cu] Atualização por classe:150224
[Lr] Data última revisão:
150224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120731
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0041092


  5 / 11 MEDLINE  
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[PMID]:20516173
[Au] Autor:Parveen Z; Mukhtar M; Pomerantz RJ
[Ti] Título:Generation of retroviral particles for the spleen necrosis virus (SNV)-based vector system and their use in transduction of various cell types.
[So] Source:Cold Spring Harb Protoc;2010(6):pdb.prot5435, 2010 Jun.
[Is] ISSN:1559-6095
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Genetically engineered retroviruses are widely used for gene delivery into human cells. A number of investigators have studied spleen necrosis virus (SNV) as a vehicle for gene delivery. Vectors developed from SNV and its closely associated avian reticuloendotheliosis virus strain A (REV-A) can be used for gene transfer into a variety of cells, including primary hematopoietic cells and human brain and post-mitotic neuronal cells that are difficult to transduce with other vector systems. SNV-based vector systems have the advantage of being quite safe, because wild-type SNV is unable to infect human cells and has less preference for integration into transcriptionally active sites or genes. However, the generation of retroviral vectors requires cotransfection of more than one plasmid into a packaging cell line, which is a tedious process. The development of stable packaging cell lines expressing envelope (Env) proteins and the structural proteins Gag-Pol will enhance mass production of retroviral vectors for future gene therapy experiments both in vitro and in vivo. This protocol describes the generation of retroviral particles for the SNV-based vector system. These particles can then be used for transduction of various cell types; as an example, a technique for transduction of post-mitotic neurons is also presented.
[Mh] Termos MeSH primário: Vetores Genéticos/genética
Vírus do Infarto Esplênico do Pato de Trager/genética
Transdução Genética/métodos
Vírion/genética
[Mh] Termos MeSH secundário: Linhagem Celular
Seres Humanos
Mitose
Neurônios/citologia
Neurônios/metabolismo
Frações Subcelulares/metabolismo
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mês de entrada:1009
[Cu] Atualização por classe:111209
[Lr] Data última revisão:
111209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100603
[St] Status:MEDLINE
[do] DOI:10.1101/pdb.prot5435


  6 / 11 MEDLINE  
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[PMID]:18942139
[Au] Autor:Venters SJ; Dias da Silva MR; Hyer J
[Ad] Endereço:Department of Neurosurgery, University of California, San Francisco, California 94143, USA.
[Ti] Título:Murine retroviruses re-engineered for lineage tracing and expression of toxic genes in the developing chick embryo.
[So] Source:Dev Dyn;237(11):3260-9, 2008 Nov.
[Is] ISSN:1058-8388
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We describe two replication incompetent retroviral vectors that co-express green fluorescent protein (GFP) and beta-galactosidase. These vectors incorporate either the avian reticuloendotheliosis (spleen necrosis virus; SNV) promoter or the chick beta-actin promoter, into the backbone of the murine leukemia (MLV) viral vector. The additional promoters drive transgene expression in avian tissue. The remainder of the vector is MLV-like, allowing high titer viral particle production by means of transient transfection. The SNV promoter produces high and early expression of introduced genes, enabling detection of the single copy integrated GFP gene in infected cells and their progeny in vivo. Substitution of the LacZ coding DNA with a relevant gene of interest will enable its co-expression with GFP, thus allowing visualization of the effect of specific and stable changes in gene expression throughout development. As the VSV-G pseudotyped viral vector is replication incompetent, changes in gene expression can be controlled temporally, by altering the timing of introduction.
[Mh] Termos MeSH primário: Vetores Genéticos/genética
Proteínas de Fluorescência Verde/genética
Vírus da Leucemia Murina de Moloney/genética
Regiões Promotoras Genéticas/genética
Transgenes/genética
beta-Galactosidase/genética
[Mh] Termos MeSH secundário: Actinas/genética
Animais
Embrião de Galinha
Expressão Gênica
Proteínas de Fluorescência Verde/biossíntese
Camundongos
Vírus do Infarto Esplênico do Pato de Trager/genética
beta-Galactosidase/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins); 147336-22-9 (Green Fluorescent Proteins); EC 3.2.1.23 (beta-Galactosidase)
[Em] Mês de entrada:0902
[Cu] Atualização por classe:161122
[Lr] Data última revisão:
161122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:081023
[St] Status:MEDLINE
[do] DOI:10.1002/dvdy.21766


  7 / 11 MEDLINE  
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[PMID]:18052720
[Au] Autor:Sinn PL; Goreham-Voss JD; Arias AC; Hickey MA; Maury W; Chikkanna-Gowda CP; McCray PB
[Ad] Endereço:Department of Pediatrics, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA. patrick-sinn@uiowa.edu
[Ti] Título:Enhanced gene expression conferred by stepwise modification of a nonprimate lentiviral vector.
[So] Source:Hum Gene Ther;18(12):1244-52, 2007 Dec.
[Is] ISSN:1043-0342
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The practical application of gene transfer as a treatment for genetic diseases such as cystic fibrosis or hemophilia has been hindered, in part, by low efficiencies of vector delivery and transgene expression. We demonstrated that a feline immunodeficiency virus (FIV)-based lentiviral vector pseudotyped with the envelope glycoprotein from the baculovirus Autographa californica (GP64) efficiently transduces and persistently expresses a reporter gene in respiratory epithelium in the absence of agents that disrupt cellular tight junction integrity. GP64-pseudotyped FIV also efficiently transduced murine hepatocytes after tail vein delivery. To improve the FIV-based vector, we tested the contribution of a series of modifications to luciferase expression in vitro and in vivo. These modifications included the addition of spleen necrosis virus U5 (SNV U5) and mutation of the major splice donor and gag start codon located in the packaging region of the FIV transgene plasmid. After vector modification, we observed significantly enhanced expression of luciferase in respiratory epithelia after nasal application and in the liver after tail vein delivery. In addition, we observed significantly enhanced human factor VIII production after tail vein delivery. These sequential modifications provide an improved FIV lentivirus platform for gene therapy applications and may be applied to other retroviral vectors.
[Mh] Termos MeSH primário: Expressão Gênica
Terapia Genética
Vetores Genéticos
Vírus da Imunodeficiência Felina/genética
Transdução Genética
[Mh] Termos MeSH secundário: Animais
Baculoviridae/genética
Códon de Iniciação/genética
Fator VIII/genética
Produtos do Gene gag/genética
Genes Reporter
Hepatócitos/enzimologia
Seres Humanos
Lentivirus/genética
Luciferases/análise
Luciferases/genética
Camundongos
Mutação
Sítios de Splice de RNA/genética
Vírus do Infarto Esplênico do Pato de Trager/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Codon, Initiator); 0 (Gene Products, gag); 0 (RNA Splice Sites); 9001-27-8 (Factor VIII); EC 1.13.12.- (Luciferases)
[Em] Mês de entrada:0802
[Cu] Atualização por classe:121115
[Lr] Data última revisão:
121115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071207
[St] Status:MEDLINE


  8 / 11 MEDLINE  
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[PMID]:17426138
[Au] Autor:Bolinger C; Yilmaz A; Hartman TR; Kovacic MB; Fernandez S; Ye J; Forget M; Green PL; Boris-Lawrie K
[Ad] Endereço:Center for Retrovirus Research, The Ohio State University, Columbus, OH 43210-1093, USA.
[Ti] Título:RNA helicase A interacts with divergent lymphotropic retroviruses and promotes translation of human T-cell leukemia virus type 1.
[So] Source:Nucleic Acids Res;35(8):2629-42, 2007.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The 5' untranslated region (UTR) of retroviruses contain structured replication motifs that impose barriers to efficient ribosome scanning. Two RNA structural motifs that facilitate efficient translation initiation despite a complex 5' UTR are internal ribosome entry site (IRES) and 5' proximal post-transcriptional control element (PCE). Here, stringent RNA and protein analyses determined the 5' UTR of spleen necrosis virus (SNV), reticuloendotheliosis virus A (REV-A) and human T-cell leukemia virus type 1 (HTLV-1) exhibit PCE activity, but not IRES activity. Assessment of SNV translation initiation in the natural context of the provirus determined that SNV is reliant on a cap-dependent initiation mechanism. Experiments with siRNAs identified that REV-A and HTLV-1 PCE modulate post-transcriptional gene expression through interaction with host RNA helicase A (RHA). Analysis of hybrid SNV/HTLV-1 proviruses determined SNV PCE facilitates Rex/Rex responsive element-independent Gag production and interaction with RHA is necessary. Ribosomal profile analyses determined that RHA is necessary for polysome association of HTLV-1 gag and provide direct evidence that RHA is necessary for efficient HTLV-1 replication. We conclude that PCE/RHA is an important translation regulatory axis of multiple lymphotropic retroviruses. We speculate divergent retroviruses have evolved a convergent RNA-protein interaction to modulate translation of their highly structured mRNA.
[Mh] Termos MeSH primário: Regiões 5´ não Traduzidas/química
Vírus 1 Linfotrópico T Humano/genética
Iniciação Traducional da Cadeia Peptídica
RNA Helicases/metabolismo
RNA Viral/química
Vírus da Reticuloendoteliose Aviária/genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Produtos do Gene gag/biossíntese
Produtos do Gene gag/genética
Provírus/genética
Provírus/metabolismo
Sequências Repetidas Terminais
Vírus do Infarto Esplênico do Pato de Trager/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (Gene Products, gag); 0 (RNA, Viral); EC 3.6.4.13 (RNA Helicases)
[Em] Mês de entrada:0706
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070412
[St] Status:MEDLINE


  9 / 11 MEDLINE  
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[PMID]:17328670
[Au] Autor:Cheng X; Cheng X; Mukhtar M; Acheampong EA; Srinivasan A; Rafi M; Pomerantz RJ; Parveen Z
[Ad] Endereço:The Dorrance H. Hamilton Laboratories, Division of Infectious Diseases and Environmental Medicine, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, Pennsylvania, USA.
[Ti] Título:HIV-1 Vpr potently induces programmed cell death in the CNS in vivo.
[So] Source:DNA Cell Biol;26(2):116-31, 2007 Feb.
[Is] ISSN:1044-5498
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The human immunodeficiency virus type I (HIV-1) accessory protein Vpr has been associated with the induction of programmed cell death (apoptosis) and cell-cycle arrest. Studies have shown the apoptotic effect of Vpr on primary and established cell lines and on diverse tissues including the central nervous system (CNS) in vitro. However, the relevance of the effect of Vpr observed in vitro to HIV-1 neuropathogenesis in vivo, remains unknown. Due to the narrow host range of HIV-1 infection, no animal model is currently available. This has prompted us to consider a small animal model to evaluate the effects of Vpr on CNS in vivo through surrogate viruses expressing HIV-1Vpr. A single round of replication competent viral vectors, expressing Vpr, were used to investigate the apoptosis-inducing capabilities of HIV-1Vpr in vivo. Viral particles pseudotyped with VSV-G or N2c envelopes were generated from spleen necrosis virus (SNV) and HIV-1-based vectors to transduce CNS cells. The in vitro studies have demonstrated that Vpr generated by SNV vectors had less apoptotic effects on CNS cells compared with Vpr expressed by HIV-1 vectors. The in vivo study has suggested that viral particles, expressing Vpr generated by HIV-1-based vectors, when delivered through the ventricle, caused loss of neurons and dendritic processes in the cortical region. The apoptotic effect was extended beyond the cortical region and affected the hippocampus neurons, the lining of the choroids plexus, and the cerebellum. However, the effect of Vpr, when delivered through the cortex, showed neuronal damage only around the site of injection. Interestingly, the number of apoptotic neurons were significantly higher with HIV-1 vectors expressing Vpr than by the SNV vectors. This may be due to the differences in the proteins expressed by these viral vectors. These results suggest that Vpr induces apoptosis in CNS cells in vitro and in vivo. To our knowledge, this is the first study to investigate the apoptosis-inducing capabilities of HIV-1Vpr in vivo in neonatal mice. We propose that this, in expensive animal model, may be of value to design-targeted neuroprotective therapeutics.
[Mh] Termos MeSH primário: Apoptose
Encéfalo/patologia
Produtos do Gene vpr/fisiologia
HIV-1/metabolismo
[Mh] Termos MeSH secundário: Animais
Animais Recém-Nascidos
Encéfalo/metabolismo
Células Cultivadas
Vetores Genéticos
Seres Humanos
Camundongos
Camundongos Endogâmicos C57BL
Oligodendroglia/patologia
Vírus do Infarto Esplênico do Pato de Trager/genética
Produtos do Gene vpr do Vírus da Imunodeficiência Humana
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Gene Products, vpr); 0 (vpr Gene Products, Human Immunodeficiency Virus)
[Em] Mês de entrada:0704
[Cu] Atualização por classe:071115
[Lr] Data última revisão:
071115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070303
[St] Status:MEDLINE


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[PMID]:17156810
[Au] Autor:Lee SK; Boyko V; Hu WS
[Ad] Endereço:HIV Drug Resistance Program, National Cancer Institute, Frederick, MD 21702, USA.
[Ti] Título:Capsid is an important determinant for functional complementation of murine leukemia virus and spleen necrosis virus Gag proteins.
[So] Source:Virology;360(2):388-97, 2007 Apr 10.
[Is] ISSN:0042-6822
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this report, we examined the abilities and requirements of heterologous Gag proteins to functionally complement each other to support viral replication. Two distantly related gammaretroviruses, murine leukemia virus (MLV) and spleen necrosis virus (SNV), were used as a model system because SNV proteins can support MLV vector replication. Using chimeric or mutant Gag proteins that could not efficiently support MLV vector replication, we determined that a homologous capsid (CA) domain was necessary for the functional complementation of MLV and SNV Gag proteins. Findings from the bimolecular fluorescence complementation assay revealed that MLV and SNV Gag proteins were capable of colocalizing and interacting in cells. Taken together, our results indicated that MLV and SNV Gag proteins can interact in cells; however, a homologous CA domain is needed for functional complementation of MLV and SNV Gag proteins to complete virus replication. This requirement of homologous Gag most likely occurs at a postassembly step(s) of the viral replication.
[Mh] Termos MeSH primário: Proteínas do Capsídeo/fisiologia
Produtos do Gene gag/fisiologia
Vírus da Leucemia Murina/fisiologia
Vírus do Infarto Esplênico do Pato de Trager/fisiologia
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Proteínas do Capsídeo/genética
Linhagem Celular
Células/química
Cães
Produtos do Gene gag/metabolismo
Teste de Complementação Genética
Seres Humanos
Microscopia Confocal
Estrutura Terciária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., INTRAMURAL
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Gene Products, gag)
[Em] Mês de entrada:0706
[Cu] Atualização por classe:161025
[Lr] Data última revisão:
161025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:061213
[St] Status:MEDLINE



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