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  1 / 192 MEDLINE  
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[PMID]:27358491
[Au] Autor:Johnson AD; Cohn CS
[Ad] Endereço:Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, USA john4613@umn.edu.
[Ti] Título:Xenotropic Murine Leukemia Virus-Related Virus (XMRV) and the Safety of the Blood Supply.
[So] Source:Clin Microbiol Rev;29(4):749-57, 2016 10.
[Is] ISSN:1098-6618
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In 2006, a new virus, xenotropic murine leukemia virus-related virus (XMRV), was discovered in a cohort of U.S. men with prostate cancer. Soon after this initial finding, XMRV was also detected in samples from patients with chronic fatigue syndrome (CFS). The blood community, which is highly sensitive to the threat of emerging infectious diseases since the HIV/AIDS crisis, recommended indefinite deferral of all blood donors with a history of CFS. As XMRV research progressed, conflicting results emerged regarding the importance of this virus in the pathophysiology of prostate cancer and/or CFS. Molecular biologists traced the development of XMRV to a recombination event in a laboratory mouse that likely occurred circa 1993. The virus was propagated via cell lines derived from a tumor present in this mouse and spread through contamination of laboratory samples. Well-controlled experiments showed that detection of XMRV was due to contaminated samples and was not a marker of or a causal factor in prostate cancer or CFS. This paper traces the development of XMRV in the prostate and CFS scientific communities and explores the effect it had on the blood community.
[Mh] Termos MeSH primário: Síndrome de Fadiga Crônica/etiologia
Neoplasias da Próstata/etiologia
Infecções por Retroviridae/complicações
Infecções por Retroviridae/transmissão
Reação Transfusional
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Sangue/virologia
Evolução Molecular
Síndrome de Fadiga Crônica/epidemiologia
Seres Humanos
Masculino
Camundongos
Neoplasias da Próstata/epidemiologia
Infecções por Retroviridae/virologia
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160701
[St] Status:MEDLINE
[do] DOI:10.1128/CMR.00086-15


  2 / 192 MEDLINE  
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[PMID]:26901817
[Au] Autor:Kirkegaard JS; Ravassard P; Ingvarsen S; Diedisheim M; Bricout-Neveu E; Grønborg M; Frogne T; Scharfmann R; Madsen OD; Rescan C; Albagli O
[Ti] Título:Xenotropic retrovirus Bxv1 in human pancreatic ß cell lines.
[So] Source:J Clin Invest;126(3):1109-13, 2016 Mar 01.
[Is] ISSN:1558-8238
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:It has been reported that endogenous retroviruses can contaminate human cell lines that have been passaged as xenotransplants in immunocompromised mice. We previously developed and described 2 human pancreatic ß cell lines (EndoC-ßH1 and EndoC-ßH2) that were generated in this way. Here, we have shown that B10 xenotropic virus 1 (Bxv1), a xenotropic endogenous murine leukemia virus (MuLV), is present in these 2 recently described cell lines. We determined that Bxv1 was also present in SCID mice that were used for in vivo propagation of EndoC-ßH1/2 cells, suggesting that contamination occurred during xenotransplantation. EndoC-ßH1/2 cells released Bxv1 particles that propagated to human 293T and Mus dunni cells. Mobilization assays demonstrated that Bxv1 transcomplements defective MuLV-based retrovectors. In contrast, common rodent ß cell lines, rat INS-1E and RIN-5F cells and mouse MIN6 and ßTC3 cells, displayed either no or extremely weak xenotropic helper activity toward MuLV-based retrovectors, although xenotropic retrovirus sequences and transcripts were detected in both mouse cell lines. Bxv1 propagation from EndoC-ßH1/2 to 293T cells occurred only under optimized conditions and was overall poorly efficient. Thus, although our data imply that MuLV-based retrovectors should be cautiously used in EndoC-ßH1/2 cells, our results indicate that an involuntary propagation of Bxv1 from these cells can be easily avoided with good laboratory practices.
[Mh] Termos MeSH primário: Células Secretoras de Insulina/virologia
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/genética
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Expressão Gênica
Genoma Viral
Xenoenxertos
Seres Humanos
Camundongos
Camundongos SCID
Ratos
Proteínas do Envelope Viral/metabolismo
Integração Viral
Replicação Viral
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Viral Envelope Proteins)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:160223
[St] Status:MEDLINE


  3 / 192 MEDLINE  
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[PMID]:26837976
[Au] Autor:Sinha M; Shafiulla M; Trupti K; Namrata NR; Nadimul H; Sabitha KS; Kumar RV; Jayshree RS
[Ad] Endereço:Department of Microbiology, Kidwai Memorial Institute of Oncology, Bangalore, Karnataka, India.
[Ti] Título:No evidence of association of xenotropic murine leukemia virus-related virus with oral cancers: Experience from a tertiary care center in South India.
[So] Source:Indian J Cancer;52(1):61-4, 2015 Jan-Mar.
[Is] ISSN:1998-4774
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Development of oral cancer, a widely prevalent cancer in India, is multifactorial with increased risk in those habituated to smoking, consuming alcohol and chewing paan and tobacco. This does not preclude other etiological factors in the causation of this cancer. Exploratory studies on several oncogenic viruses have found varied associations with oral cancers. AIM: The aim of this study was to explore the association of xenotropic murine leukemia virus-related virus, (XMRV) a retrovirus recently implicated in oncogenesis in humans, with oral cancers. SETTINGS AND DESIGN: The presence of XMRV proviral deoxyribonucleic acid (DNA) was evaluated by standard nucleic acid amplification from DNA extracted from representative bits of tumor tissues and adjacent normal tissues from surgically resected specimens sent post-operatively for routine histopathological testing. MATERIALS AND METHODS: This prospective study comprised 109 patients with a provisional diagnosis of oral cancer who were operated at the Oral Oncology Department of Kidwai Memorial Institute of Oncology, over a period of 10 months. RESULTS: XMRV was not found in any of the tumor tissues (squamous cell carcinomas - 98; verrucous carcinomas - 4) nor in any of the normal tissues. It is thus important that the absence of this oncogenic virus in all the cases makes the association of XMRV with oral cancers very unlikely. CONCLUSIONS: There is a need to investigate potentially oncogenic viruses in other solid tumors and in larger sample sizes. Any such association could have implications in detecting, preventing and treating these cancers.
[Mh] Termos MeSH primário: Neoplasias Bucais/patologia
Neoplasias Bucais/virologia
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/patogenicidade
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Feminino
Seres Humanos
Índia
Masculino
Camundongos
Meia-Idade
Vírus Oncogênicos/patogenicidade
Estudos Prospectivos
Centros de Atenção Terciária
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160204
[St] Status:MEDLINE
[do] DOI:10.4103/0019-509X.175595


  4 / 192 MEDLINE  
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[PMID]:26282858
[Au] Autor:Hartmann S; Hasenkamp N; Mayer J; Michaux J; Morand S; Mazzoni CJ; Roca AL; Greenwood AD
[Ad] Endereço:Institute of Biochemistry and Biology, University of Potsdam, Karl-Liebknecht-Str 22-24, Potsdam, 14476, Germany. stefanie.hartmann@uni-potsdam.de.
[Ti] Título:Endogenous murine leukemia retroviral variation across wild European and inbred strains of house mouse.
[So] Source:BMC Genomics;16:613, 2015 Aug 18.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Endogenous murine leukemia retroviruses (MLVs) are high copy number proviral elements difficult to comprehensively characterize using standard low throughput sequencing approaches. However, high throughput approaches generate data that is challenging to process, interpret and present. RESULTS: Next generation sequencing (NGS) data was generated for MLVs from two wild caught Mus musculus domesticus (from mainland France and Corsica) and for inbred laboratory mouse strains C3H, LP/J and SJL. Sequence reads were grouped using a novel sequence clustering approach as applied to retroviral sequences. A Markov cluster algorithm was employed, and the sequence reads were queried for matches to specific xenotropic (Xmv), polytropic (Pmv) and modified polytropic (Mpmv) viral reference sequences. CONCLUSIONS: Various MLV subtypes were more widespread than expected among the mice, which may be due to the higher coverage of NGS, or to the presence of similar sequence across many different proviral loci. The results did not correlate with variation in the major MLV receptor Xpr1, which can restrict exogenous MLVs, suggesting that endogenous MLV distribution may reflect gene flow more than past resistance to infection.
[Mh] Termos MeSH primário: Vírus da Leucemia Murina/classificação
Vírus da Leucemia Murina/genética
RNA Viral/análise
Análise de Sequência de RNA/métodos
[Mh] Termos MeSH secundário: Animais
Europa (Continente)
Evolução Molecular
Fluxo Gênico
Vírus da Leucemia Murina/isolamento & purificação
Cadeias de Markov
Camundongos
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150819
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-015-1766-z


  5 / 192 MEDLINE  
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[PMID]:25683459
[Au] Autor:Venkiteshwaran A; Fogle J; Patnaik P; Kowle R; Chen D
[Ad] Endereço:Dept. of Bioproduct Research, Bioproduct Research and Development, Lilly Research Laboratories, Eli Lilly and Company, DC3941 Lilly Corporate Center, Indianapolis, Indiana.
[Ti] Título:Mechanistic evaluation of virus clearance by depth filtration.
[So] Source:Biotechnol Prog;31(2):431-7, 2015 Mar-Apr.
[Is] ISSN:1520-6033
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Virus clearance by depth filtration has not been well-understood mechanistically due to lack of quantitative data on filter charge characteristics and absence of systematic studies. It is generally believed that both electrostatic interactions and sized based mechanical entrapment contribute to virus clearance by depth filtration. In order to establish whether the effectiveness of virus clearance correlates with the charge characteristics of a given depth filter, a counter-ion displacement technique was employed to determine the ionic capacity for several depth filters. Two depth filters (Millipore B1HC and X0HC) with significant differences in ionic capacities were selected and evaluated for their ability to eliminate viruses. The high ionic capacity X0HC filter showed complete porcine parvovirus (PPV) clearance (eliminating the spiked viruses to below the limit of detection) under low conductivity conditions (≤2.5 mS/cm), achieving a log10 reduction factor (LRF) of > 4.8. On the other hand, the low ionic capacity B1HC filter achieved only ∼2.1-3.0 LRF of PPV clearance under the same conditions. These results indicate that parvovirus clearance by these two depth filters are mainly achieved via electrostatic interactions between the filters and PPV. When much larger xenotropic murine leukemia virus (XMuLV) was used as the model virus, complete retrovirus clearance was obtained under all conditions evaluated for both depth filters, suggesting the involvement of mechanisms other than just electrostatic interactions in XMuLV clearance.
[Mh] Termos MeSH primário: Filtração/instrumentação
Filtração/métodos
Vírus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Gatos
Linhagem Celular
Concentração Osmolar
Parvovirus Suíno
Eletricidade Estática
Suínos
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150423
[Lr] Data última revisão:
150423
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150217
[St] Status:MEDLINE
[do] DOI:10.1002/btpr.2061


  6 / 192 MEDLINE  
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[PMID]:25335906
[Au] Autor:Bach J; Connell-Crowley L
[Ad] Endereço:Drug Substance Development, Amgen Inc., 1201 Amgen Court West, Seattle, 98119, Washington.
[Ti] Título:Clearance of the rodent retrovirus, XMuLV, by protein A chromatography.
[So] Source:Biotechnol Bioeng;112(4):743-50, 2015 Apr.
[Is] ISSN:1097-0290
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Protein A chromatography is the most common unit operation used in the manufacture of therapeutic monoclonal antibodies (mAbs) due to its high affinity and specificity for the IgG Fc domain. However, protein A chromatography is often not effective for viral clearance. Typical log reduction values (LRV) for the model retrovirus XMuLV range between 1 and 4 logs, while effective steps such as viral filtration can achieve 5-7 logs of clearance. XMuLV LRVs obtained on protein A are reproducible for a given mAb, but can vary widely for different mAbs, even with the same operating conditions. In order to understand the mechanism of XMuLV clearance on protein A, we have investigated its partitioning on Mabselect SuRe protein A resin and explored how the virus interacts with resin, product, and impurities. The results show that XMuLV has some interaction with the resin backbone and ligand, but also appears to bind to and coelute with the mAb. The interaction with product was further examined by evaluating the effect of feed conditions, loading, and different washes on XMuLV partitioning on the column. Understanding the mechanism of XMuLV removal on a protein A, resin provides insight into the variability and low viral clearance of this step and suggests ways in which the removal of virus by this step can be improved.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/isolamento & purificação
Cromatografia de Afinidade/métodos
Proteína Estafilocócica A/metabolismo
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/isolamento & purificação
[Mh] Termos MeSH secundário: Meios de Cultura/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Culture Media); 0 (Staphylococcal Protein A)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150302
[Lr] Data última revisão:
150302
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141023
[St] Status:MEDLINE
[do] DOI:10.1002/bit.25484


  7 / 192 MEDLINE  
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Covas, Dimas Tadeu
[PMID]:25387292
[Au] Autor:Slavov SN; Otaguiri KK; Macedo MD; Rocha-Júnior MC; Silva-Pinto AC; Kashima S; Covas DT
[Ad] Endereço:Regional Blood Center of Ribeirão Preto, Faculty of Medicine of Ribeirão Preto, University of São Paulo-USP, Ribeirão Preto, São Paulo, Brazil.
[Ti] Título:No evidence of xenotropic murine leukemia virus-related virus infection in Brazilian multiply transfused patients with sickle cell disease and beta-thalassemia major.
[So] Source:New Microbiol;37(4):543-50, 2014 Oct.
[Is] ISSN:1121-7138
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:Although xenotropic murine leukemia virus-related virus (XMRV) has been regarded as a laboratory contaminant, it remains one of the most controversial viruses. The objective of the study was to determine if XMRV is present in 44 patients with beta-thalassemia major, 48 with sickle cell disease, and 89 volunteer blood donors. After RNA/ DNA extraction from plasma/buffy coat the samples were screened for XMRV sequences by conserved nested GAG primers. None of the RNA samples showed a positive result. Surprisingly, four DNA samples obtained from blood donors were positive for XMRV provirus. The subsequent phylogenetic analysis revealed that these sequences are identical to the positive control (murine leukemia retrovirus) and are probably consistent with laboratory contamination. XMRV infection (provirus and viral RNA) was absent in multiply transfused patients and volunteer blood donors. The positive result obtained from some blood donors probably reflects laboratory contamination. We believe that XMRV does not pose risk to blood transfusion.
[Mh] Termos MeSH primário: Anemia Falciforme/virologia
Infecções por Retroviridae/virologia
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/isolamento & purificação
Talassemia beta/virologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Doadores de Sangue
Transfusão de Sangue
Brasil
Criança
Pré-Escolar
Feminino
Seres Humanos
Masculino
Camundongos
Meia-Idade
Dados de Sequência Molecular
Filogenia
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/classificação
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/genética
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1501
[Cu] Atualização por classe:141112
[Lr] Data última revisão:
141112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141112
[St] Status:MEDLINE


  8 / 192 MEDLINE  
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[PMID]:25260582
[Au] Autor:Mbemba G; Henry E; Delelis O; Bouger MC; Buckle M; Mouscadet JF; Hazan U; Leh H; Bury-Moné S
[Ad] Endereço:LBPA, CNRS, Ecole Normale Supérieure (ENS) de Cachan, 61 avenue du Président Wilson, 94235 Cachan, France. Electronic address: gladys.mbemba@lbpa.ens-cachan.fr.
[Ti] Título:Biochemical properties of the xenotropic murine leukemia virus-related virus integrase.
[So] Source:Biochimie;107 Pt B:300-9, 2014 Dec.
[Is] ISSN:1638-6183
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Xenotropic Murine Leukemia Virus-related Virus (XMRV) is a new gammaretrovirus generated by genetic recombination between two murine endogenous retroviruses, PreXMRV1 and PreXMRV2, during passaging of human prostate cancer xenografts in laboratory mice. XMRV is representative of an early founder virus that jumps species from mouse to human cell lines. Relatively little information is available concerning the XMRV integrase (IN), an enzyme that catalyzes a key stage in the retroviral cycle, and whose sequence is conserved among replication competent retroviruses emerging from recombination between the murine endogenous PreXMRV-1 and PreXMRV-2 genomes. Previous studies have shown that IN inhibitors efficiently block XMRV multiplication in cells. We thus aimed at characterizing the biochemical properties and sensitivity of the XMRV IN to the raltegravir, dolutegravir, 118-D-24 and elvitegravir inhibitors in vitro. We report for the first time the purification and enzymatic characterization of recombinant XMRV IN. This IN, produced in Escherichia coli and purified under native conditions, is optimally active over a pH range of 7-8.5, in the presence of Mg(2+) (15 mM and 30 mM for 3'-processing and strand transfer, respectively) and is poorly sensitive to the addition of dithiothreitol. Raltegravir was shown to be a very potent inhibitor (IC50 âˆ¼ 30 nM) whereas dolutegravir and elvitegravir were less effective (IC50 âˆ¼ 230 nM and 650 nM, respectively). The 118-D-24 drug had no impact on XMRV IN activity. Interestingly, the substrate specificity of XMRV IN seems to be less marked compared to HIV-1 IN since XMRV IN is able to process various donor substrates that share little homology. Finally, our analysis revealed some original properties of the XMRV IN such as its relatively low sequence specificity.
[Mh] Termos MeSH primário: Inibidores de Integrase/farmacologia
Integrases/química
Integrases/metabolismo
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Ditiotreitol/farmacologia
Integrase de HIV/química
Compostos Heterocíclicos com 3 Anéis/farmacologia
Concentração de Íons de Hidrogênio
Integrases/genética
Integrases/isolamento & purificação
Dados de Sequência Molecular
Pirrolidinonas/farmacologia
Quinolonas/farmacologia
Raltegravir Potássico
Proteínas Recombinantes/genética
Proteínas Recombinantes/isolamento & purificação
Homologia de Sequência de Aminoácidos
Especificidade por Substrato
Proteínas Virais/química
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Heterocyclic Compounds, 3-Ring); 0 (Integrase Inhibitors); 0 (JTK 303); 0 (Pyrrolidinones); 0 (Quinolones); 0 (Recombinant Proteins); 0 (Viral Proteins); 0 (p31 integrase protein, Human immunodeficiency virus 1); 43Y000U234 (Raltegravir Potassium); DKO1W9H7M1 (dolutegravir); EC 2.7.7.- (HIV Integrase); EC 2.7.7.- (Integrases); T8ID5YZU6Y (Dithiothreitol)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140928
[St] Status:MEDLINE


  9 / 192 MEDLINE  
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[PMID]:25233966
[Au] Autor:Baig FA; Mirza T; Khanani R; Khan S
[Ad] Endereço:Departments of Histopathology, Dow Diagnostic Research and Reference Laboratory, Dow University of Health Sciences, Karachi.
[Ti] Título:Detection of Xenotropic murine leukemia virus-related virus in prostate biopsy samples.
[So] Source:J Coll Physicians Surg Pak;24(9):636-9, 2014 Sep.
[Is] ISSN:1681-7168
[Cp] País de publicação:Pakistan
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To determine the association of Xenotropic murine leukemia virus related virus (XMRV) infection with prostate cancer and compare it with benign prostate hyperplasia. STUDY DESIGN: Case control study. PLACE AND DURATION OF STUDY: Department of Histopathology and Molecular Pathology, Dow University of Health Sciences, Karachi, from January 2009 to December 2012. METHODOLOGY: XMRV was screened in 50 prostate cancer and 50 benign prostatic hyperplasia biopsies using conventional end-point PCR. Other studied variables were family history of prostate cancer, patients age and Gleason score. RESULTS: XMRV was detected in 4 (8%) of the 50 prostate cancer biopsy specimens compared to none in biopsies with benign prostatic hyperplasia. However, there was no significant statistical association of XMRV infection with the other variables. CONCLUSION: A low frequency of XMRV infection was found in this case-control study. Men, who harbor XMRV infection, may be at increased risk of prostate cancer but this needs to be investigated further at a larger scale.
[Mh] Termos MeSH primário: Adenocarcinoma/virologia
Próstata/virologia
Hiperplasia Prostática/virologia
Neoplasias da Próstata/virologia
Infecções Tumorais por Vírus/epidemiologia
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/isolamento & purificação
[Mh] Termos MeSH secundário: Adenocarcinoma/epidemiologia
Adenocarcinoma/patologia
Idoso
Idoso de 80 Anos ou mais
Biópsia
Estudos de Casos e Controles
Seres Humanos
Masculino
Meia-Idade
Gradação de Tumores
Paquistão/epidemiologia
Reação em Cadeia da Polimerase
Próstata/patologia
Hiperplasia Prostática/epidemiologia
Hiperplasia Prostática/patologia
Neoplasias da Próstata/epidemiologia
Neoplasias da Próstata/patologia
Provírus/genética
RNA Viral/genética
RNA Viral/isolamento & purificação
Infecções Tumorais por Vírus/virologia
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:140919
[Lr] Data última revisão:
140919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140920
[St] Status:MEDLINE
[do] DOI:09.2014/JCPSP.636639


  10 / 192 MEDLINE  
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[PMID]:25190459
[Au] Autor:Pilkington GR; Purzycka KJ; Bear J; Le Grice SF; Felber BK
[Ad] Endereço:Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA.
[Ti] Título:Gammaretrovirus mRNA expression is mediated by a novel, bipartite post-transcriptional regulatory element.
[So] Source:Nucleic Acids Res;42(17):11092-106, 2014.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Post-transcriptional regulatory mechanisms of several complex and simple retroviruses and retroelements have been elucidated, with the exception of the gammaretrovirus family. We found that, similar to the other retroviruses, gag gene expression of MuLV and XMRV depends on post-transcriptional regulation mediated via an RNA sequence overlapping the pro-pol open reading frame, termed the Post-Transcriptional Element (PTE). PTE function can be replaced by heterologous RNA export elements, e.g. CTE of simian type D retroviruses. Alternatively, Gag particle production is achieved using an RNA/codon optimized gag gene. PTE function is transferable and can replace HIV Rev-RRE-regulated expression of HIV gag. Analysis of PTE by SHAPE revealed a highly structured RNA comprising seven stem-loop structures, with the 5' and 3' stem-loops forming an essential bipartite signal. MuLV and XMRV PTE share 98% identity and have highly similar RNA structures, with changes mostly located to single-stranded regions. PTE identification strongly suggests that all retroviruses and retroelements share common strategies of post-transcriptional gene regulation to produce Gag. Expression depends on complex RNA structures embedded within retroviral mRNA, in coding regions or the 3' untranslated region. These specific structures serve as recognition signals for either cellular or viral proteins.
[Mh] Termos MeSH primário: Regulação Viral da Expressão Gênica
Vírus da Leucemia Murina de Moloney/genética
RNA Mensageiro/química
RNA Viral/química
Sequências Reguladoras de Ácido Ribonucleico
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/genética
[Mh] Termos MeSH secundário: Produtos do Gene gag/genética
Produtos do Gene gag/metabolismo
Células HEK293
Células HeLa
Seres Humanos
Vírus da Leucemia Murina de Moloney/metabolismo
Conformação de Ácido Nucleico
RNA Mensageiro/metabolismo
Vírus Relacionado ao Vírus Xenotrópico da Leucemia Murina/metabolismo
Produtos do Gene gag do Vírus da Imunodeficiência Humana/genética
Produtos do Gene gag do Vírus da Imunodeficiência Humana/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., INTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Gene Products, gag); 0 (RNA, Messenger); 0 (RNA, Viral); 0 (Regulatory Sequences, Ribonucleic Acid); 0 (gag Gene Products, Human Immunodeficiency Virus)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140906
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gku798



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