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  1 / 33 MEDLINE  
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[PMID]:22776774
[Au] Autor:Tenaya IW; Heel K; Stumbles PA; Wilcox GE
[Ad] Endereço:School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
[Ti] Título:Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.
[So] Source:Vet Immunol Immunopathol;149(3-4):167-76, 2012 Oct 15.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Jembrana disease virus (JDV) is an unusual bovine lentivirus that causes an acute and sometimes fatal disease after a short incubation period in Bali cattle (Bos javanicus). The pathological changes occur primarily in lymphoid tissues, which feature proliferating lymphoblastoid-like cells predominantly throughout parafollicular (T-cell) areas, and atrophy of follicles (B-cell) areas. Five Bali cattle were experimentally infected with JDV and all developed typical clinical signs of Jembrana disease characterised by a transient febrile response, enlargement of superficial lymph nodes and a significant leukopenia. Flow cytometric analysis of PBMC during the acute (febrile) disease phase showed that the reduced number of lymphocytes was due to a significant decrease in both the proportion and absolute numbers of CD4(+) T cells, but not CD8(+) T-cells or CD21(+) B-cells. At the end of the febrile phase, total numbers of both CD8(+) T-cells and CD21(+) B-cells increased significantly, while CD4(+) T-cell numbers remained below normal values, resulting in a significantly reduced CD4(+):CD8(+) ratio. We speculate that the persistent depletion of CD4(+) T cells following JDV infection, through lack of CD4(+) T cell help to B cells, may explain the lack of production of JDV-specific antibodies for several weeks after recovery despite an increase in CD21(+) B cell numbers. Further, our previous data showing that IgG(+) plasma cells are targets for JDV infection, correlated with our current data demonstrating an increase in CD8(+) T cell numbers, supports the suggestion that anti-viral cytotoxic T cell or other cell-mediated immune responses may be critical in the recovery process, although this remains to be formally demonstrated for JDV.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD8-Positivos/imunologia
Doenças dos Bovinos/virologia
Infecções por Lentivirus/veterinária
Lentivirus Bovinos/imunologia
Subpopulações de Linfócitos/imunologia
[Mh] Termos MeSH secundário: Animais
Relação CD4-CD8/veterinária
Linfócitos T CD4-Positivos/citologia
Linfócitos T CD8-Positivos/citologia
Bovinos
Doenças dos Bovinos/imunologia
Feminino
Citometria de Fluxo/veterinária
Imunofenotipagem/veterinária
Indonésia
Interferon gama/sangue
Infecções por Lentivirus/imunologia
Infecções por Lentivirus/virologia
Leucócitos Mononucleares/citologia
Leucócitos Mononucleares/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:120919
[Lr] Data última revisão:
120919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120711
[St] Status:MEDLINE


  2 / 33 MEDLINE  
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[PMID]:20570311
[Au] Autor:McNab T; Desport M; Dobson R; Tenaya IW; Hartaningsih N; Wilcox GE
[Ad] Endereço:School of Veterinary and Biomedical Science, Murdoch University, Murdoch WA 6150, Australia. tegan.mcnab@gmail.com
[Ti] Título:Prior bovine immunodeficiency virus infection does not inhibit subsequent superinfection by the acutely pathogenic Jembrana disease virus.
[So] Source:Virology;404(2):261-8, 2010 Sep 01.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In cattle the interaction between the two genetically and antigenically related bovine lentiviruses, the acutely pathogenic Jembrana disease virus (JDV) and the non-pathogenic Bovine immunodeficiency virus (BIV) has not been reported although both JDV and a BIV-like virus have been reported in the Bali cattle (Bos javanicus) population in Indonesia. The outcome of infection of Bali cattle with the R29 strain of BIV prior to superinfection 42 days later with JDV(TAB/87) was determined. All BIV-inoculated cattle were successfully infected and developed an antibody response to the TM and CA proteins. BIV infection did not prevent subsequent infection with JDV or ameliorate the clinical signs of Jembrana disease in the infected cattle. It did, however, modify the dynamics of the JDV infection with an earlier onset and end of the acute disease process, and a reduction in the duration of viremia that exceeded 10(6) genome copies/ml of plasma.
[Mh] Termos MeSH primário: Doenças dos Bovinos/imunologia
Doenças dos Bovinos/virologia
Infecções por Lentivirus/veterinária
Lentivirus Bovinos/imunologia
[Mh] Termos MeSH secundário: Animais
Temperatura Corporal
Bovinos
Ensaio de Imunoadsorção Enzimática
Vírus da Imunodeficiência Bovina/imunologia
Infecções por Lentivirus/imunologia
Infecções por Lentivirus/virologia
Fatores de Tempo
Carga Viral
Viremia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1008
[Cu] Atualização por classe:100712
[Lr] Data última revisão:
100712
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100624
[St] Status:MEDLINE
[do] DOI:10.1016/j.virol.2010.05.001


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[PMID]:20210780
[Au] Autor:Desport M; Lewis J
[Ad] Endereço:School of Veterinary and Biomedical Science, Murdoch University, Perth, WA 6150, Australia. mdesport@murdoch.edu.au
[Ti] Título:Jembrana disease virus: host responses, viral dynamics and disease control.
[So] Source:Curr HIV Res;8(1):53-65, 2010 Jan.
[Is] ISSN:1873-4251
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Jembrana disease virus (JDV) is the most recently discovered member of the lentivirus family and causes an acute clinical disease in Bali cattle with a fatality rate of approximately 15%. It is genetically related to bovine immunodeficiency virus (BIV) to the extent that infections cannot yet be differentially diagnosed using serological assays due to cross-reacting epitopes. Despite their close genetic relationship the pathogenesis of JDV infection in Bali cattle is very different to that of BIV in cattle and is unusual for a member of this virus family. The dynamics of JDV replication and clearance during the acute stage of Jembrana disease, the viral tropism, molecular analysis of the viral genome and mRNA transcripts, and the current status of vaccine development and diagnostic assays are all reviewed to provide a greater understanding of the factors that make JDV such an unusual lentivirus.
[Mh] Termos MeSH primário: Infecções por Lentivirus/veterinária
Lentivirus Bovinos
[Mh] Termos MeSH secundário: Animais
Vetores Artrópodes
Búfalos
Bovinos
Indonésia
Infecções por Lentivirus/prevenção & controle
Infecções por Lentivirus/virologia
Lentivirus Bovinos/genética
Lentivirus Bovinos/imunologia
Lentivirus Bovinos/patogenicidade
Filogenia
Carga Viral
Tropismo Viral
Vacinas Virais/uso terapêutico
Vírion/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Viral Vaccines)
[Em] Mês de entrada:1007
[Cu] Atualização por classe:100309
[Lr] Data última revisão:
100309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100310
[St] Status:MEDLINE


  4 / 33 MEDLINE  
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[PMID]:19733380
[Au] Autor:Desport M; Tenaya IW; McLachlan A; McNab TJ; Rachmat J; Hartaningsih N; Wilcox GE
[Ad] Endereço:School of Veterinary and Biomedical Science, Murdoch University, Murdoch WA 6150, Australia. mdesport@murdoch.edu.au
[Ti] Título:In vivo infection of IgG-containing cells by Jembrana disease virus during acute infection.
[So] Source:Virology;393(2):221-7, 2009 Oct 25.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Jembrana disease virus (JDV) is an unusual bovine lentivirus which causes a non-follicular proliferation of lymphocytes, a transient immunosuppression and a delayed humoral response in infected Bali cattle in Indonesia. A double-immunofluorescent labeling method was developed to identify the subset of mononuclear cells in which the viral capsid protein could be detected. Viral antigen was present in pleomorphic centroblast-like cells which were identified as IgG-containing cells, including plasma cells, in lymphoid tissues. There was no evidence of infection of CD3(+) T-cells or MAC387(+) monocytes in tissues but large vacuolated cells with a macrophage-like morphology in the lung were found to contain viral antigen although they could not be shown conclusively to be infected. The tropism of JDV for mature IgG-containing cells may be relevant to understanding the pathogenesis of Jembrana disease, the delayed antibody responses and the genetic composition of this atypical lentivirus.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Doenças dos Bovinos/imunologia
Imunoglobulina G/imunologia
Infecções por Lentivirus/veterinária
Lentivirus Bovinos/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/imunologia
Antígenos Virais/imunologia
Proteínas do Capsídeo/imunologia
Bovinos
Doenças dos Bovinos/virologia
Feminino
Infecções por Lentivirus/imunologia
Infecções por Lentivirus/virologia
Pulmão/imunologia
Pulmão/virologia
Tecido Linfoide/imunologia
Tecido Linfoide/virologia
Macrófagos/imunologia
Macrófagos/virologia
Monócitos/imunologia
Monócitos/virologia
Plasmócitos/imunologia
Plasmócitos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (Capsid Proteins); 0 (Immunoglobulin G)
[Em] Mês de entrada:0910
[Cu] Atualização por classe:091019
[Lr] Data última revisão:
091019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090908
[St] Status:MEDLINE
[do] DOI:10.1016/j.virol.2009.07.027


  5 / 33 MEDLINE  
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[PMID]:19442849
[Au] Autor:Lewis J; McNab T; Tenaya M; Hartaningsih N; Wilcox G; Desport M
[Ad] Endereço:School of Veterinary and Biomedical Sciences, Murdoch University, South St., Murdoch, WA 6150, Australia.
[Ti] Título:Comparison of immunoassay and real-time PCR methods for the detection of Jembrana disease virus infection in Bali cattle.
[So] Source:J Virol Methods;159(1):81-6, 2009 Jul.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A sensitive diagnostic assay for the detection of infections with the bovine lentivirus Jembrana disease virus (JDV) is required in Indonesia to control the spread of Jembrana disease. Immunoassays are used routinely but are compromised by cross-reactive epitopes in the capsid (CA) protein of JDV and the genetically related bovine immunodeficiency virus (BIV). JDV gag-specific primers were tested in a real-time PCR assay to detect proviral DNA in peripheral blood mononuclear cells from 165 cattle from the Tabanan district of Bali. JDV-specific amplicons were detected in 9% of the cattle and only 33% of the real-time PCR positive cattle were seropositive. The delayed seroconversion that occurs after infection with JDV could explain the low concordance between these assays but other factors may be responsible. BIV proviral DNA was not detected in any of the PBMC DNA samples. A high concordance value of 98.6% was found between the JDV plasma-derived antigen Western blot and the JDV p26-his recombinant protein ELISA. Only 21% of the seropositive cattle had detectable levels of proviral DNA suggesting that the proviral load in recovered cattle is low. A combination of real-time PCR and JDV p26-his ELISA is recommended for the detection of infection with JDV in Indonesia.
[Mh] Termos MeSH primário: Doenças dos Bovinos/diagnóstico
Imunoensaio
Infecções por Lentivirus/veterinária
Lentivirus Bovinos/isolamento & purificação
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Anticorpos Antivirais/imunologia
Antígenos Virais/imunologia
Sequência de Bases
Western Blotting
Bovinos
Doenças dos Bovinos/sangue
Doenças dos Bovinos/imunologia
DNA Viral/sangue
Ensaio de Imunoadsorção Enzimática
Indonésia
Infecções por Lentivirus/sangue
Infecções por Lentivirus/diagnóstico
Infecções por Lentivirus/imunologia
Lentivirus Bovinos/genética
Dados de Sequência Molecular
Proteínas Recombinantes de Fusão/imunologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (DNA, Viral); 0 (Recombinant Fusion Proteins)
[Em] Mês de entrada:0906
[Cu] Atualização por classe:090515
[Lr] Data última revisão:
090515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090516
[St] Status:MEDLINE
[do] DOI:10.1016/j.jviromet.2009.03.005


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[PMID]:19261319
[Au] Autor:Ditcham WG; Lewis JR; Dobson RJ; Hartaningsih N; Wilcox GE; Desport M
[Ad] Endereço:School of Veterinary and Biomedical Science, Murdoch University, Perth, Western Australia 6150, Australia.
[Ti] Título:Vaccination reduces the viral load and the risk of transmission of Jembrana disease virus in Bali cattle.
[So] Source:Virology;386(2):317-24, 2009 Apr 10.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The efficacy of a tissue-derived vaccine, which is currently used in Indonesia to control the spread of Jembrana disease in Bali cattle, was determined by quantifying the viral load in plasma following experimental infection with Jembrana disease virus. Virus transmission is most likely to occur during the acute phase of infection when viral titers are greater than 10(6) genomes/ml. Vaccinated cattle were found to have a 96% reduction in viral load above this threshold compared to control cattle. This would reduce the chance of virus transmission as the number of days above the threshold in the vaccinated cattle was reduced by 33%. Viral loads at the onset and resolution of fever were significantly lower in the vaccinated cattle and immune function was maintained with the development of antibody responses to Env proteins within 10-24 days post challenge. There was, however, no significant reduction in the duration of the febrile period in vaccinated animals. The duration and severity of clinical parameters were found to be variable within each group of cattle but the quantification of viral load revealed the benefits of vaccinating to reduce the risk of virus transmission as well as to ameliorate disease.
[Mh] Termos MeSH primário: Doenças dos Bovinos/prevenção & controle
Infecções por Lentivirus/veterinária
Lentivirus Bovinos/imunologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Anticorpos Antivirais/imunologia
Bovinos
Doenças dos Bovinos/imunologia
Doenças dos Bovinos/virologia
Feminino
Imunoglobulina G/sangue
Imunoglobulina G/imunologia
Infecções por Lentivirus/imunologia
Infecções por Lentivirus/prevenção & controle
Infecções por Lentivirus/virologia
Modelos Lineares
RNA Viral/análise
Vacinação/veterinária
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Immunoglobulin G); 0 (RNA, Viral); 0 (Viral Vaccines)
[Em] Mês de entrada:0904
[Cu] Atualização por classe:090330
[Lr] Data última revisão:
090330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090306
[St] Status:MEDLINE
[do] DOI:10.1016/j.virol.2009.02.008


  7 / 33 MEDLINE  
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[PMID]:19230948
[Au] Autor:Desport M; Ditcham WG; Lewis JR; McNab TJ; Stewart ME; Hartaningsih N; Wilcox GE
[Ad] Endereço:School of Veterinary and Biomedical Science, Murdoch University, Perth, WA 6150, Australia. mdesport@murdoch.edu.au
[Ti] Título:Analysis of Jembrana disease virus replication dynamics in vivo reveals strain variation and atypical responses to infection.
[So] Source:Virology;386(2):310-6, 2009 Apr 10.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Jembrana disease virus (JDV) is an acute lentiviral infection of Bali cattle in Indonesia. Data generated during a series of cattle infection experiments was examined and significant differences were identified in the mean plasma viral load on the first and second days of the febrile response in cattle infected with JDV(TAB/87) compared to those infected with JDV(PUL/01). The peak and total viral loads >or=10(6) genome copies/ml during the acute stage of the disease were significantly higher in JDV(TAB/87) infected cattle. JDV(PUL/01) infected cattle developed peak rectal temperatures earlier than the JDV(TAB/87) cattle but there were no differences in the duration of the febrile responses observed for the 2 groups of animals. The plasma viremia was above 10(6) genome copies/ml for almost 3 days longer in JDV(TAB/87) compared to JDV(PUL/01) infected cattle. Atypical responses to infection occurred in approximately 15% of experimentally infected animals, characterized by reduced viral loads, lower or absent febrile responses and absence of p26-specific antibody responses. Most of these cattle developed normal Tm-specific antibody responses between 4-12 weeks post-infection.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Infecções por Lentivirus/veterinária
Lentivirus Bovinos/fisiologia
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/imunologia
Temperatura Corporal
Bovinos
Doenças dos Bovinos/imunologia
Feminino
Infecções por Lentivirus/imunologia
Infecções por Lentivirus/virologia
Lentivirus Bovinos/genética
Lentivirus Bovinos/imunologia
RNA Viral/genética
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (RNA, Viral)
[Em] Mês de entrada:0904
[Cu] Atualização por classe:090330
[Lr] Data última revisão:
090330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:090224
[St] Status:MEDLINE
[do] DOI:10.1016/j.virol.2009.01.014


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[PMID]:18828067
[Au] Autor:Mintern JD; Guillonneau C
[Ad] Endereço:The University of Melbourne, Department of Microbiology and Immunology, Parkville, Victoria 3010, Australia. jmintern@unimelb.edu.au
[Ti] Título:Frontiers in immunology research network--2008 International Conference.
[So] Source:IDrugs;11(10):710-2, 2008 Oct.
[Is] ISSN:1369-7056
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Alergia e Imunologia/tendências
Pesquisa Biomédica/tendências
[Mh] Termos MeSH secundário: Animais
Anexina A2/análise
Antineoplásicos/uso terapêutico
Doenças Autoimunes/tratamento farmacológico
Doenças Autoimunes/imunologia
Vacinas Anticâncer/uso terapêutico
Bovinos
Doenças dos Bovinos/prevenção & controle
Doenças dos Bovinos/virologia
Ensaio de Imunoadsorção Enzimática/tendências
Seres Humanos
Imunoterapia/tendências
Interleucina-2/uso terapêutico
Lentivirus Bovinos/imunologia
Listeria monocytogenes/imunologia
Neoplasias Hepáticas/química
Peptídeos/uso terapêutico
Proteínas Recombinantes/uso terapêutico
Vacinas Virais
[Pt] Tipo de publicação:CONGRESSES
[Nm] Nome de substância:
0 (Annexin A2); 0 (Antineoplastic Agents); 0 (Cancer Vaccines); 0 (Interleukin-2); 0 (Peptides); 0 (Recombinant Proteins); 0 (Viral Vaccines)
[Em] Mês de entrada:0810
[Cu] Atualização por classe:081001
[Lr] Data última revisão:
081001
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:081002
[St] Status:MEDLINE


  9 / 33 MEDLINE  
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[PMID]:18466992
[Au] Autor:Stewart ME; Desport M; Setiyaningsih S; Hartaningsih N; Wilcox GE
[Ad] Endereço:School of Veterinary and Biomedical Science, Murdoch University, Murdoch, South Street, Perth, WA 6150, Australia.
[Ti] Título:Analysis of Jembrana disease virus mRNA transcripts produced during acute infection demonstrates a complex transcription pattern.
[So] Source:Virus Res;135(2):336-9, 2008 Aug.
[Is] ISSN:0168-1702
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Jembrana disease virus (JDV) is an unusual bovine lentivirus that causes an acute disease syndrome with a 20% case fatality rate after a short incubation period in Bos javanicus (Bali cattle) in Indonesia. Analysis of tat mRNA transcription patterns has identified up to six differently spliced transcripts indicating that, in common with other lentiviruses, JDV uses a complex splicing pattern. RT-PCR analysis of mRNA transcripts produced during the acute phase of infection with JDV(TAB/87) revealed at least 12 differently spliced transcripts involving 9 different splice sites. A single unspliced gag/pol transcript, singly spliced vif and tmx specific transcripts and alternatively spliced env, tat and rev transcripts were identified. A 67 nucleotide putative non-coding exon was identified that shared the same splice acceptor (SA) as vif and was incorporated into alternative transcripts of tat, rev and env.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Infecções por Lentivirus/veterinária
Lentivirus Bovinos/genética
Lentivirus Bovinos/patogenicidade
RNA Mensageiro/metabolismo
Transcrição Genética
[Mh] Termos MeSH secundário: Doença Aguda
Animais
Sequência de Bases
Bovinos
Produtos do Gene tat/genética
Produtos do Gene tat/metabolismo
Infecções por Lentivirus/virologia
Lentivirus Bovinos/classificação
Lentivirus Bovinos/metabolismo
Dados de Sequência Molecular
Sítios de Splice de RNA
Processamento de RNA
RNA Mensageiro/química
RNA Mensageiro/genética
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Gene Products, tat); 0 (RNA Splice Sites); 0 (RNA, Messenger); 0 (Viral Proteins)
[Em] Mês de entrada:0809
[Cu] Atualização por classe:080624
[Lr] Data última revisão:
080624
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080510
[St] Status:MEDLINE
[do] DOI:10.1016/j.virusres.2008.03.017


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[PMID]:17656359
[Au] Autor:Xuan C; Qiao W; Gao J; Liu M; Zhang X; Cao Y; Chen Q; Geng Y; Zhou J
[Ad] Endereço:College of Life Sciences, Nankai University, Tianjin 300071, China.
[Ti] Título:Regulation of microtubule assembly and stability by the transactivator of transcription protein of Jembrana disease virus.
[So] Source:J Biol Chem;282(39):28800-6, 2007 Sep 28.
[Is] ISSN:0021-9258
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Microtubules are cytoskeletal polymers consisting of tubulin subunits that take part in diverse cell activities. Many viruses hijack cellular motor proteins to move on microtubules toward the cell interior during the entry process and toward the plasma membrane during the egress period. In addition, viruses often remodel microtubules to facilitate the generation of infectious progeny. In this study, we found that the transactivator of transcription protein of Jembrana disease virus (Jtat) bound tubulin and microtubules both in cells and in the purified system. Microtubule co-sedimentation and co-localization assays revealed a robust interaction of Jtat with microtubules. Tubulin turbidity assay further showed that Jtat promoted tubulin polymerization in vitro in a concentration-dependent manner. Moreover, Jtat promoted the partitioning of cellular tubulin toward the polymeric form, increased the level of tubulin acetylation, and significantly enhanced the cold stability of cellular microtubules. In addition, Jtat-mediated disruption of microtubule dynamics induced the release of Bim from microtubules, leading to profound apoptosis. These results not only identify Jtat as an important viral regulator of microtubule dynamics but also indicate that Jtat-induced apoptosis might contribute to Jembrana disease pathogenesis.
[Mh] Termos MeSH primário: Apoptose
Lentivirus Bovinos/fisiologia
Microtúbulos/metabolismo
Transativadores/metabolismo
Tubulina (Proteína)/metabolismo
Internalização do Vírus
[Mh] Termos MeSH secundário: Acetilação
Animais
Proteínas Reguladoras de Apoptose/química
Proteínas Reguladoras de Apoptose/metabolismo
Proteína 11 Semelhante a Bcl-2
Bovinos
Linhagem Celular
Sistema Livre de Células
Seres Humanos
Lentivirus Bovinos/química
Lentivirus Bovinos/patogenicidade
Proteínas de Membrana/química
Proteínas de Membrana/metabolismo
Microtúbulos/química
Microtúbulos/virologia
Proteínas Proto-Oncogênicas/química
Proteínas Proto-Oncogênicas/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/metabolismo
Transativadores/química
Tubulina (Proteína)/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins); 0 (BCL2L11 protein, human); 0 (Bcl-2-Like Protein 11); 0 (Membrane Proteins); 0 (Proto-Oncogene Proteins); 0 (Recombinant Proteins); 0 (Trans-Activators); 0 (Tubulin)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:161124
[Lr] Data última revisão:
161124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070728
[St] Status:MEDLINE



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