Base de dados : MEDLINE
Pesquisa : B04.820.650.589.520 [Categoria DeCS]
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  1 / 4 MEDLINE  
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[Au] Autor:Qian L; Han X; Liu X
[Ad] Endereço:State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, 300071, China.
[Ti] Título:Structural insight into equine lentivirus receptor 1.
[So] Source:Protein Sci;24(5):633-42, 2015 May.
[Is] ISSN:1469-896X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Equine lentivirus receptor 1 (ELR1) has been identified as a functional cellular receptor for equine infectious anemia virus (EIAV). Herein, recombinant ELR1 and EIAV surface glycoprotein gp90 were respectively expressed in Drosophila melanogaster S2 cells, and purified to homogeneity by Ni-NTA affinity chromatography and gel filtration chromatography. Gel filtration chromatography and analytical ultracentrifugation (AUC) analyses indicated that both ELR1 and gp90 existed as individual monomers in solution and formed a complex with a stoichiometry of 1:1 when mixed. The structure of ELR1 was first determined with the molecular replacement method, which belongs to the space group P42 21 2 with one molecule in an asymmetric unit. It contains eight antiparallel ß-sheets, of which four are in cysteine rich domain 1 (CRD1) and two are in CRD2 and CRD3, respectively. Alignment of ELR1 with HVEM and CD134 indicated that Tyr61, Leu70, and Gly72 in CRD1 of ELR1 are important residues for binding to gp90. Isothermal titration calorimetry (ITC) experiments further confirmed that Leu70 and Gly72 are the critical residues.
[Mh] Termos MeSH primário: Lentivirus Equinos/química
Glicoproteínas de Membrana/química
Estrutura Secundária de Proteína
Receptores Virais/química
Proteínas Recombinantes/química
[Mh] Termos MeSH secundário: Animais
Drosophila melanogaster
Anemia Infecciosa Equina/genética
Anemia Infecciosa Equina/virologia
Vírus da Anemia Infecciosa Equina/patogenicidade
Glicoproteínas de Membrana/biossíntese
Glicoproteínas de Membrana/genética
Complexos Multiproteicos/química
Proteínas Recombinantes/biossíntese
Proteínas Recombinantes/genética
[Nm] Nome de substância:
0 (Membrane Glycoproteins); 0 (Multiprotein Complexes); 0 (Receptors, Virus); 0 (Recombinant Proteins)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:160501
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150107
[St] Status:MEDLINE
[do] DOI:10.1002/pro.2634

  2 / 4 MEDLINE  
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[Au] Autor:Donofrio G; Capocefalo A; Franceschi V; Morini G; Del Bue M; Conti V; Cavirani S; Grolli S
[Ad] Endereço:Dipartimento di Salute Animale, Sezione di Malattie Infettive, Facoltà di Medicina Veterinaria, via del Taglio 10, 43100 Parma, Italy.
[Ti] Título:Virally and physically transgenized equine adipose-derived stromal cells as a cargo for paracrine secreted factors.
[So] Source:BMC Cell Biol;11:73, 2010 Sep 23.
[Is] ISSN:1471-2121
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Adipose-Derived Stromal Cells have been shown to have multiple lineage differentiation properties and to be suitable for tissues regeneration in many degenerative processes. Their use has been proposed for the therapy of joint diseases and tendon injuries in the horse. In the present report the genetic manipulation of Equine Adipose-Derived Stromal Cells has been investigated. RESULTS: Equine Adipose-Derived Stromal Cells were successfully virally transduced as well as transiently and stably transfected with appropriate parameters, without detrimental effect on their differentiation properties. Moreover, green fluorescent protein alone, fused to neo gene, or co-expressed as bi-cistronic reporter constructs, driven by viral and house-keeping gene promoters, were tested. The better expressed cassette was employed to stably transfect Adipose-Derived Stromal Cells for cell therapy purposes. Stably transfected Equine Adipose-Derived Stromal Cells with a heterologous secreted viral antigen were able to immunize horses upon injection into the lateral wall of the neck. CONCLUSION: This study provides the methods to successfully transgenize Adipose-Derived Stromal Cells both by lentiviral vector and by transfection using optimized constructs with suitable promoters and reporter genes. In conclusion these findings provide a working platform for the delivery of potentially therapeutic proteins to the site of cells injection via transgenized Equine Adipose-Derived Stromal Cells.
[Mh] Termos MeSH primário: Artropatias/terapia
Infecções por Lentivirus/terapia
Células Estromais/transplante
Transdução Genética/métodos
[Mh] Termos MeSH secundário: Tecido Adiposo/citologia
Antígenos Virais/genética
Antígenos Virais/imunologia
Antígenos Virais/metabolismo
Diferenciação Celular/genética
Clonagem Molecular
Estudos de Viabilidade
Terapia Genética
Regeneração Tecidual Guiada
Infecções por Lentivirus/genética
Infecções por Lentivirus/imunologia
Lentivirus Equinos
Transplante de Células-Tronco
Células Estromais/imunologia
Células Estromais/metabolismo
Células Estromais/patologia
[Nm] Nome de substância:
0 (Antigens, Viral)
[Em] Mês de entrada:1103
[Cu] Atualização por classe:141202
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100925
[St] Status:MEDLINE
[do] DOI:10.1186/1471-2121-11-73

  3 / 4 MEDLINE  
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[Au] Autor:Craigo JK; Zhang B; Barnes S; Tagmyer TL; Cook SJ; Issel CJ; Montelaro RC
[Ad] Endereço:Center for Vaccine Research, Department of Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, PA 15261, USA.
[Ti] Título:Envelope variation as a primary determinant of lentiviral vaccine efficacy.
[So] Source:Proc Natl Acad Sci U S A;104(38):15105-10, 2007 Sep 18.
[Is] ISSN:0027-8424
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lentiviral envelope antigenic variation and associated immune evasion are believed to present major obstacles to effective vaccine development. Although this perception is widely assumed by the scientific community, there is, to date, no rigorous experimental data assessing the effect of increasing levels of lentiviral Env variation on vaccine efficacy. It is our working hypothesis that Env is, in fact, a primary determinant of vaccine effectiveness. We previously reported that a successful experimental attenuated equine infectious anemia virus vaccine, derived by mutation of the viral S2 accessory gene, provided 100% protection from disease after virulent virus challenge. Here, we sought to comprehensively test our hypothesis by challenging vaccinated animals with proviral strains of defined, increasing Env variation, using variant envelope SU genes that arose naturally during experimental infection of ponies with equine infectious anemia virus. The reference attenuated vaccine combined with these variant Env challenge strains facilitated evaluation of the protection conferred by ancestral immunogens, because the Env of the attenuated vaccine is a direct ancestor to the variant proviral strain Envs. The results demonstrated that ancestral Env proteins did not impart broad levels of protection against challenge. Furthermore, the results displayed a significant inverse linear correlation of Env divergence and protection from disease. This study demonstrates potential obstacles to the use of single isolate ancestral Env immunogens. Finally, these findings reveal that relatively minor Env variation can pose a substantial challenge to lentiviral vaccine immunity, even when attenuated vaccines are used that, to date, achieve the highest levels of vaccine protection.
[Mh] Termos MeSH primário: Variação Antigênica
Produtos do Gene env/imunologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/imunologia
Anemia Infecciosa Equina/imunologia
Anemia Infecciosa Equina/prevenção & controle
Vírus da Anemia Infecciosa Equina/imunologia
Lentivirus Equinos/patogenicidade
Fatores de Tempo
Proteínas Virais/imunologia
Vacinas Virais/administração & dosagem
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Gene Products, env); 0 (Viral Proteins); 0 (Viral Vaccines)
[Em] Mês de entrada:0711
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070912
[St] Status:MEDLINE

  4 / 4 MEDLINE  
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[Au] Autor:Fraser DG; Mealey RH; McGuire TC
[Ad] Endereço:Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164-7040, USA.
[Ti] Título:Selecting peptides to optimize Th1 responses to an equine lentivirus using HLA-DR binding motifs and defined HIV-1 Th peptides.
[So] Source:Immunogenetics;55(7):508-14, 2003 Oct.
[Is] ISSN:0093-7711
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three moderately to broadly recognized equine infectious anemia virus (EIAV) peptides that contained helper T-lymphocyte (Th) 1 epitopes were previously identified. Although lipopeptide immunization was only weakly immunostimulatory in a preliminary study, as measured by T-lymphocyte proliferation responses, it was of interest to define additional broadly recognized Th1 epitopes to include in future immunization trials. Using broadly cross-reactive and conserved Th epitopes known in the related human immunodeficiency virus-1 (HIV-1) and binding motifs defined in human leukocyte antigen DR molecules as guides, this work identified three new peptides containing Th1 epitopes recognized by 60-75% of EIAV infected horses. The observed similarity across species of major histocompatibility complex (MHC) class II binding motifs and the conservation of Th peptides between related viruses should allow easier targeting of Th epitope regions in less well characterized pathogens and/or in species whose MHC class II molecules are poorly defined.
[Mh] Termos MeSH primário: Proteínas de Ligação a DNA/imunologia
Antígenos HLA-DR/imunologia
Lentivirus Equinos/imunologia
Fatores de Transcrição/imunologia
[Mh] Termos MeSH secundário: Animais
Fatores de Transcrição Hélice-Alça-Hélice Básicos
Seres Humanos
[Nm] Nome de substância:
0 (Basic Helix-Loop-Helix Transcription Factors); 0 (DNA-Binding Proteins); 0 (HLA-DR Antigens); 0 (Hand1 protein, mouse); 0 (Peptides); 0 (Transcription Factors)
[Em] Mês de entrada:0312
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:030828
[St] Status:MEDLINE

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