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  1 / 11 MEDLINE  
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[PMID]:23982166
[Au] Autor:Condiotti R; Goldenberg D; Giladi H; Schnitzer-Perlman T; Waddington SN; Buckley SM; Heim D; Cheung W; Themis M; Coutelle C; Simerzin A; Osejindu E; Wege H; Themis M; Galun E
[Ad] Endereço:Goldyne Savad Institute of Gene Therapy, Hadassah Hebrew University Medical Center, Jerusalem, Israel.
[Ti] Título:Transduction of fetal mice with a feline lentiviral vector induces liver tumors which exhibit an E2F activation signature.
[So] Source:Mol Ther;22(1):59-68, 2014 Jan.
[Is] ISSN:1525-0024
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lentiviral vectors are widely used in basic research and clinical applications for gene transfer and long-term expression; however, safety issues have not yet been completely resolved. In this study, we characterized hepatocarcinomas that developed in mice 1 year after in utero administration of a feline-derived lentiviral vector. Mapped viral integration sites differed among tumors and did not coincide with the regions of chromosomal aberrations. Furthermore, gene expression profiling revealed that no known cancer-associated genes were deregulated in the vicinity of viral integrations. Nevertheless, five of the six tumors exhibited highly significant upregulation of E2F target genes, of which a majority are associated with oncogenesis, DNA damage response, and chromosomal instability. We further show in vivo and in vitro that E2F activation occurs early on following transduction of both fetal mice and cultured human hepatocytes. On the basis of the similarities in E2F target gene expression patterns among tumors and the lack of evidence implicating insertional mutagenesis, we propose that transduction of fetal mice with a feline lentiviral vector induces E2F-mediated major cellular processes that drive hepatocytes toward uncontrolled proliferation culminating in tumorigenesis.
[Mh] Termos MeSH primário: Fatores de Transcrição E2F/metabolismo
Feto
Vetores Genéticos/genética
Lentivirus Felinos/genética
Neoplasias Hepáticas/etiologia
Transdução Genética
[Mh] Termos MeSH secundário: Animais
Gatos
Transformação Celular Neoplásica/genética
Aberrações Cromossômicas
Dano ao DNA
Dosagem de Genes
Expressão Gênica
Regulação da Expressão Gênica
Seres Humanos
Neoplasias Hepáticas/metabolismo
Camundongos
Mutagênese Insercional
Transcriptoma
Transgenes
Integração Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (E2F Transcription Factors)
[Em] Mês de entrada:1408
[Cu] Atualização por classe:170120
[Lr] Data última revisão:
170120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130829
[St] Status:MEDLINE
[do] DOI:10.1038/mt.2013.193


  2 / 11 MEDLINE  
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[PMID]:24006716
[Au] Autor:Mestrinho LA; Runhau J; Bragança M; Niza MM
[Ad] Endereço:Centre of Research in Veterinary Sciences, Faculty of Veterinary Medicine, Lusófona University of Humanities and Technologies, Lisboa, Portugal. p3125@ulusofona.pt
[Ti] Título:Risk assessment of feline tooth resorption: a Portuguese clinical case control study.
[So] Source:J Vet Dent;30(2):78-83, 2013.
[Is] ISSN:0898-7564
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tooth resorption (TR) is one of the most common dental diseases in cats. Determination of risk factors has not yet been fully assessed and, to the best knowledge of the authors, this disease has never been studied in Portuguese cats. The objective of this case-control study was to determine type and distribution of TR lesions, evaluate risk factors, and establish relationships between variables in this disease. The study included data from 71 cats admitted for general anesthesia for various reasons. The cats were randomly selected. The inclusion criteria were availability of clinical history and owner permission. Cats with known oral disease were not excluded from the study. All cats received ultrasonic scaling and polishing of the teeth, a thorough oral examination, and full-mouth radiographs. A strong statistical relation was found between age and TR. The age group of 10 to 15-years showed an increased risk of 6.56 times for TR occurrence compared with the group 0 to 4-years of age. Presence of gingivitis in all index levels was related to an increased risk for TR. No relation was found between age or gingivitis index and lesion type. Mandibular third premolar and molar teeth were most commonly affected by TR, especially for type 1 lesions. Canine teeth were statistically more likely to have type 2 lesions. The trend for the canine teeth to be more affected with type 2 lesions needs further verification.
[Mh] Termos MeSH primário: Doenças do Gato/etiologia
Reabsorção de Dente/veterinária
[Mh] Termos MeSH secundário: Fatores Etários
Animais
Dente Pré-Molar/patologia
Estudos de Casos e Controles
Gatos
Dente Canino/patologia
Profilaxia Dentária/veterinária
Raspagem Dentária/veterinária
Feminino
Hemorragia Gengival/complicações
Hemorragia Gengival/veterinária
Hipertrofia Gengival/complicações
Hipertrofia Gengival/veterinária
Gengivite/classificação
Gengivite/complicações
Gengivite/veterinária
Vírus da Imunodeficiência Felina/isolamento & purificação
Infecções por Lentivirus/complicações
Infecções por Lentivirus/veterinária
Lentivirus Felinos/isolamento & purificação
Masculino
Dente Molar/patologia
Portugal
Radiografia Dentária/veterinária
Medição de Risco
Reabsorção de Dente/etiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1312
[Cu] Atualização por classe:170810
[Lr] Data última revisão:
170810
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:130907
[St] Status:MEDLINE


  3 / 11 MEDLINE  
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[PMID]:21813188
[Au] Autor:Dietrich I; McEwan WA; Hosie MJ; Willett BJ
[Ad] Endereço:Centre for Virus Research, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Bearsden Road, Glasgow G61 1QH, United Kingdom.
[Ti] Título:Restriction of the felid lentiviruses by a synthetic feline TRIM5-CypA fusion.
[So] Source:Vet Immunol Immunopathol;143(3-4):235-42, 2011 Oct 15.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Gene therapy approaches to the treatment of HIV infection have targeted both viral gene expression and the cellular factors that are essential for virus replication. However, significant concerns have been raised regarding the potential toxic effects of such therapies, the emergence of resistant viral variants and unforeseen biological consequences such as enhanced susceptibility to unrelated pathogens. Novel restriction factors formed by the fusion of the tripartite motif protein (TRIM5) and cyclophilin A (CypA), or "TRIMCyps", offer an effective antiviral defence strategy with a very low potential for toxicity. In order to investigate the potential therapeutic utility of TRIMCyps in gene therapy for AIDS, a synthetic fusion protein between feline TRIM5 and feline CypA was generated and transduced into cells susceptible to infection with feline immunodeficiency virus (FIV). The synthetic feline TRIMCyp was highly efficient at preventing infection with both HIV and FIV and the cells resisted productive infection with FIV from either the domestic cat or the puma. Feline TRIMCyp and FIV infection of the cat offers a unique opportunity to evaluate TRIMCyp-based approaches to genetic therapy for HIV infection and the treatment of AIDS.
[Mh] Termos MeSH primário: Fusão Gênica Artificial
Proteínas de Transporte/genética
Ciclofilina A/genética
Lentivirus Felinos/genética
Proteínas Recombinantes de Fusão/metabolismo
[Mh] Termos MeSH secundário: Síndrome de Imunodeficiência Adquirida/terapia
Animais
Gatos/virologia
Terapia Genética
Seres Humanos
Vírus da Imunodeficiência Felina/genética
Proteínas Recombinantes de Fusão/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Carrier Proteins); 0 (Recombinant Fusion Proteins); 0 (TRIM5 protein, human); EC 5.2.1.- (Cyclophilin A)
[Em] Mês de entrada:1112
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110805
[St] Status:MEDLINE
[do] DOI:10.1016/j.vetimm.2011.06.017


  4 / 11 MEDLINE  
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[PMID]:21715025
[Au] Autor:Poeschla EM
[Ad] Endereço:Department of Molecular Medicine and Division of Infectious Diseases, Guggenheim 18, College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA. emp@mayo.edu
[Ti] Título:Primate and feline lentiviruses in current intrinsic immunity research: the cat is back.
[So] Source:Vet Immunol Immunopathol;143(3-4):215-20, 2011 Oct 15.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Retroviral restriction factor research is explaining long-standing lentiviral mysteries. Asking why a particular retrovirus cannot complete a critical part of its life cycle in cells of a particular species has been the starting point for numerous discoveries, including heretofore elusive functions of HIV-1 accessory genes. The potential for therapeutic application is substantial. Analyzing the feline immunodeficiency virus (FIV) life cycle has been instrumental and the source of some surprising observations in this field. FIV is restricted in cells of various primates by several restriction factors including APOBEC3 proteins and, uniquely, TRIM proteins from both Old and New World monkeys. In contrast, the feline genome does not encode functional TRIM5alpha or TRIMCyp proteins and HIV-1 is primarily blocked in feline cells by APOBEC3 proteins. These can be overcome by inserting FIV vif or even SIVmac vif into HIV-1. The domestic cat and its lentivirus are positioned to offer strategic research opportunities as the field moves forward.
[Mh] Termos MeSH primário: Infecções por Lentivirus/veterinária
Lentivirus Felinos/imunologia
Lentivirus de Primatas/imunologia
Doenças dos Macacos/virologia
[Mh] Termos MeSH secundário: Animais
Gatos
Síndrome de Imunodeficiência Adquirida Felina/virologia
Vírus da Imunodeficiência Felina/imunologia
Infecções por Lentivirus/imunologia
Doenças dos Macacos/imunologia
Filogenia
Vírion/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1112
[Cu] Atualização por classe:161122
[Lr] Data última revisão:
161122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110701
[St] Status:MEDLINE
[do] DOI:10.1016/j.vetimm.2011.06.014


  5 / 11 MEDLINE  
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[PMID]:19896724
[Au] Autor:Münk C; Hechler T; Chareza S; Löchelt M
[Ad] Endereço:Clinic for Gasteroenterology, Hepatology and Infectiology, Heinrich-Heine-University, Düsseldorf, Germany.
[Ti] Título:Restriction of feline retroviruses: lessons from cat APOBEC3 cytidine deaminases and TRIM5alpha proteins.
[So] Source:Vet Immunol Immunopathol;134(1-2):14-24, 2010 Mar 15.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The interplay between viral and cellular factors determines the outcome of an initial contact between a given virus and its natural host or upon encounter of a novel host. Thus, the potential of inducing disease as well as crossing host species barriers are the consequences of the molecular interactions between the parasite and its susceptible, tolerant or resistant host. Cellular restriction factors, for instance APOBEC3 and TRIM5 proteins, targeting defined pathogens or groups of pathogens as well as viral genes counter-acting these cellular defense systems are of prime importance in this respect and may even represent novel targets for prevention and therapy of virus infections. Due to the importance of host-encoded antiviral restriction and viral counter-defense for pathogenicity and host tropism, the responsible molecular factors and mechanisms are currently under intense investigation. In this review we will introduce host restriction and retroviral counter-defense systems with a special emphasis on the cat and its naturally occurring exogenous retroviruses which is a valid model for human disease, a model that will contribute to increase our basic understanding and potential applications of these important aspects of host-virus interaction.
[Mh] Termos MeSH primário: Proteínas de Transporte/fisiologia
Doenças do Gato/virologia
Citosina Desaminase/fisiologia
Infecções por Retroviridae/veterinária
Retroviridae/fisiologia
[Mh] Termos MeSH secundário: Animais
Gatos/virologia
Interações Hospedeiro-Patógeno/fisiologia
Vírus da Imunodeficiência Felina/fisiologia
Lentivirus Felinos/fisiologia
Vírus da Leucemia Felina/fisiologia
Infecções por Retroviridae/virologia
Integração Viral/fisiologia
Replicação Viral/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Carrier Proteins); EC 3.5.4.1 (Cytosine Deaminase)
[Em] Mês de entrada:1005
[Cu] Atualização por classe:100215
[Lr] Data última revisão:
100215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:091110
[St] Status:MEDLINE
[do] DOI:10.1016/j.vetimm.2009.10.004


  6 / 11 MEDLINE  
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[PMID]:16678276
[Au] Autor:Dunham SP
[Ad] Endereço:Retrovirus Research Laboratory, Institute of Comparative Medicine, Faculty of Veterinary Medicine, University of Glasgow, Bearsden Road, Glasgow G61 1QH, United Kingdom. s.dunham@vet.gla.ac.uk
[Ti] Título:Lessons from the cat: development of vaccines against lentiviruses.
[So] Source:Vet Immunol Immunopathol;112(1-2):67-77, 2006 Jul 15.
[Is] ISSN:0165-2427
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Feline immunodeficiency virus (FIV) is a natural infection of domestic cats, which produces a disease with many similarities to human immunodeficiency virus (HIV) infection in man. The virus is an important cause of morbidity and mortality in pet cats worldwide. As such an effective vaccine is desirable both for its use in veterinary medicine and also as a model for the development of an HIV vaccine. A large number of candidate vaccines have been tested against feline immunodeficiency virus. These include inactivated virus and infected cell vaccines, DNA and viral vectored vaccines, subunit and peptide vaccines and vaccines using bacterial vectors. Ultimately, the development of inactivated virus and infected cell vaccines led to the release of the first licensed vaccine against FIV, in 2002. This review highlights some of the difficulties associated with the development of lentiviral vaccines and some of the lessons that have been learned in the FIV model that are of particular relevance to the development of HIV vaccines.
[Mh] Termos MeSH primário: Doenças do Gato/prevenção & controle
Infecções por Lentivirus/veterinária
Lentivirus Felinos/imunologia
Vacinas Virais/isolamento & purificação
[Mh] Termos MeSH secundário: Vacinas contra a AIDS/isolamento & purificação
Animais
Doenças do Gato/imunologia
Gatos
Síndrome de Imunodeficiência Adquirida Felina/imunologia
Síndrome de Imunodeficiência Adquirida Felina/prevenção & controle
Variação Genética
Seres Humanos
Vírus da Imunodeficiência Felina/genética
Vírus da Imunodeficiência Felina/imunologia
Infecções por Lentivirus/imunologia
Infecções por Lentivirus/prevenção & controle
Vacinação/efeitos adversos
Vacinação/veterinária
Vacinas de DNA/isolamento & purificação
Vacinas de Produtos Inativados/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (AIDS Vaccines); 0 (Vaccines, DNA); 0 (Vaccines, Inactivated); 0 (Viral Vaccines)
[Em] Mês de entrada:0607
[Cu] Atualização por classe:081121
[Lr] Data última revisão:
081121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060509
[St] Status:MEDLINE


  7 / 11 MEDLINE  
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[PMID]:16120451
[Au] Autor:Smirnova N; Troyer JL; Schissler J; Terwee J; Poss M; VandeWoude S
[Ad] Endereço:Department of Microbiology, Immunology, and Pathology, Colorado State University, 1619 Campus Delivery, Fort Collins, CO 80523-1619, USA.
[Ti] Título:Feline lentiviruses demonstrate differences in receptor repertoire and envelope structural elements.
[So] Source:Virology;342(1):60-76, 2005 Nov 10.
[Is] ISSN:0042-6822
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Feline immunodeficiency virus (FIV) causes fatal disease in domestic cats via T cell depletion-mediated immunodeficiency. Pumas and lions are hosts for apparently apathogenic lentiviruses (PLV, LLV) distinct from FIV. We compared receptor use among these viruses by: (1) evaluating target cell susceptibility; (2) measuring viral replication following exposure to specific and non-specific receptor antagonists; and (3) comparing Env sequence and structural motifs. Most isolates of LLV and PLV productively infected domestic feline T cells, but differed from domestic cat FIV by infecting cells independent of CXCR4, demonstrating equivalent or enhanced replication following heparin exposure, and demonstrating substantial divergence in amino acid sequence and secondary structure in Env receptor binding domains. PLV infection was, however, inhibited by CD134/OX40 antibody. Thus, although PLV and LLV infection interfere with FIV superinfection, we conclude that LLV and PLV utilize novel, more promiscuous mechanisms for cell entry than FIV, underlying divergent tropism and biological properties of these viruses.
[Mh] Termos MeSH primário: Lentivirus Felinos/patogenicidade
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Gatos
Linhagem Celular
DNA Viral/genética
Glicosilação
Vírus da Imunodeficiência Felina/genética
Vírus da Imunodeficiência Felina/imunologia
Vírus da Imunodeficiência Felina/patogenicidade
Vírus da Imunodeficiência Felina/fisiologia
Lentivirus Felinos/genética
Lentivirus Felinos/imunologia
Lentivirus Felinos/fisiologia
Leões
Dados de Sequência Molecular
Puma
Receptores CXCR4/antagonistas & inibidores
Receptores CXCR4/fisiologia
Receptores OX40
Receptores do Fator de Necrose Tumoral/antagonistas & inibidores
Receptores Virais/antagonistas & inibidores
Receptores Virais/fisiologia
Homologia de Sequência de Aminoácidos
Linfócitos T/imunologia
Linfócitos T/virologia
Proteínas do Envelope Viral/química
Proteínas do Envelope Viral/genética
Proteínas do Envelope Viral/fisiologia
Virulência
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Receptors, CXCR4); 0 (Receptors, OX40); 0 (Receptors, Tumor Necrosis Factor); 0 (Receptors, Virus); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:0512
[Cu] Atualização por classe:140325
[Lr] Data última revisão:
140325
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050827
[St] Status:MEDLINE


  8 / 11 MEDLINE  
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[PMID]:15362656
[Au] Autor:Kennedy JM; Hoke A; Zhu Y; Johnston JB; van Marle G; Silva C; Zochodne DW; Power C
[Ad] Endereço:Department of Clinical Neurosciences, University of Calgary, Calgary, AB, Canada.
[Ti] Título:Peripheral neuropathy in lentivirus infection: evidence of inflammation and axonal injury.
[So] Source:AIDS;18(9):1241-50, 2004 Jun 18.
[Is] ISSN:0269-9370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: As distal sensory polyneuropathy (DSP) is a major neurological complication of HIV-1 infection, we investigated the extent of peripheral nervous system disease in animals infected with the lentivirus, feline immunodeficiency virus (FIV), because it causes neurological disease and immunosuppression in cats similar to HIV-1 in humans. METHODS: After infection with a neurovirulent FIV molecular clone, neurobehavioral testing, nerve morphology, viral detection and load measurements were performed. RESULTS: Neurobehavioral studies showed delayed withdrawal in response to a noxious stimulus among FIV-infected animals compared with sham-infected controls (P < 0.05). Dorsal root ganglia and sciatic nerves from FIV-infected ammals showed activated macrophages that were increased in number and size compared with controls. In addition, TNF-alpha messenger RNA was detectable in most nerves and spinal cords from the FIV-infected group, but was infrequently detected in controls. Viral RNA copy numbers in plasma and sciatic nerves were detectable in all FIV-infected animals at high levels. Studies of sural nerves identified myelinated fiber atrophy in 12-week FIV-infected animals compared with age-matched control animals, which was accompanied by reduced myelin sheath thickness (P < 0.05). The footpads of FIV-infected animals displayed reduced intraepidermal fiber density compared with control animals (P < 0.01). CONCLUSION: FIV infection results in the rapid onset of peripheral neuropathy, defined by axonal injury and macrophage activation, together with abundant virus within the nerve, indicating that it may serve as a model of HIV-related DSP.
[Mh] Termos MeSH primário: Doenças do Gato/imunologia
Infecções por Lentivirus/imunologia
Lentivirus Felinos
Doenças do Sistema Nervoso Periférico/virologia
[Mh] Termos MeSH secundário: Animais
Axônios/patologia
Comportamento Animal
Doenças do Gato/patologia
Gatos
Feminino
Gânglios Espinais/imunologia
Infecções por Lentivirus/patologia
Ativação de Macrófagos
Fibras Nervosas Mielinizadas/patologia
Fibras Nervosas Amielínicas/patologia
Doenças do Sistema Nervoso Periférico/patologia
Nervo Isquiático/imunologia
Nervo Sural/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, P.H.S.
[Em] Mês de entrada:0411
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
061115
[Sb] Subgrupo de revista:IM; X
[Da] Data de entrada para processamento:040915
[St] Status:MEDLINE


  9 / 11 MEDLINE  
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[PMID]:15240147
[Au] Autor:Condiotti R; Curran MA; Nolan GP; Giladi H; Ketzinel-Gilad M; Gross E; Galun E
[Ad] Endereço:Goldyne Savad Institute of Gene Therapy, Hadassah University Hospital, Jerusalem 91120, Israel. reba@hadassah.org.il
[Ti] Título:Prolonged liver-specific transgene expression by a non-primate lentiviral vector.
[So] Source:Biochem Biophys Res Commun;320(3):998-1006, 2004 Jul 30.
[Is] ISSN:0006-291X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Liver-directed gene therapy has the potential for treatment of numerous inherited diseases affecting metabolic functions. The aim of this study was to evaluate gene expression in hepatocytes using feline immunodeficiency virus-based lentiviral vectors, which may be potentially safer than those based on human immunodeficiency virus. In vitro studies revealed that gene expression was stable for up to 24 days post-transduction and integration into the host cell genome was suggested by Alu PCR and Southern blot analyses. Systemic in vivo administration of viral particles by the hydrodynamics method resulted in high levels of gene expression exclusively in the liver for over 7 months whereas injection of plasmid DNA by the same method led to transient expression levels. Our studies suggest that feline immunodeficiency-based lentiviral vectors specifically transduce liver cells and may be used as a novel vehicle of gene delivery for treatment of metabolic disease.
[Mh] Termos MeSH primário: Carcinoma Hepatocelular/genética
Regulação Neoplásica da Expressão Gênica/genética
Vírus da Imunodeficiência Felina/genética
Transfecção/métodos
[Mh] Termos MeSH secundário: Animais
Gatos
Linhagem Celular Tumoral
Síndrome de Imunodeficiência Adquirida Felina/genética
Síndrome de Imunodeficiência Adquirida Felina/virologia
Terapia Genética/métodos
Vetores Genéticos/genética
Seres Humanos
Lentivirus Felinos/genética
Primatas
Transgenes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:0408
[Cu] Atualização por classe:121115
[Lr] Data última revisão:
121115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:040709
[St] Status:MEDLINE


  10 / 11 MEDLINE  
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[PMID]:11333894
[Au] Autor:Browning MT; Schmidt RD; Lew KA; Rizvi TA
[Ad] Endereço:Department of Veterinary Sciences, The University of Texas M.D. Anderson Cancer Center, Bastrop, Texas 78602, USA.
[Ti] Título:Primate and feline lentivirus vector RNA packaging and propagation by heterologous lentivirus virions.
[So] Source:J Virol;75(11):5129-40, 2001 Jun.
[Is] ISSN:0022-538X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Development of safe and effective gene transfer systems is critical to the success of gene therapy protocols for human diseases. Currently, several primate lentivirus-based gene transfer systems, such as those based on human and simian immunodeficiency viruses (HIV/SIV), are being tested; however, their use in humans raises safety concerns, such as the generation of replication-competent viruses through recombination with related endogenous retroviruses or retrovirus-like elements. Due to the greater phylogenetic distance from primate lentiviruses, feline immunodeficiency virus (FIV) is becoming the lentivirus of choice for human gene transfer systems. However, the safety of FIV-based vector systems has not been tested experimentally. Since lentiviruses such as HIV-1 and SIV have been shown to cross-package their RNA genomes, we tested the ability of FIV RNA to get cross-packaged into primate lentivirus particles such as HIV-1 and SIV, as well as a nonlentiviral retrovirus such as Mason-Pfizer monkey virus (MPMV), and vice versa. Our results reveal that FIV RNA can be cross-packaged by primate lentivirus particles such as HIV-1 and SIV and vice versa; however, a nonlentivirus particle such as MPMV is unable to package FIV RNA. Interestingly, FIV particles can package MPMV RNA but cannot propagate the vector RNA further for other steps of the retrovirus life cycle. These findings reveal that diverse retroviruses are functionally more similar than originally thought and suggest that upon coinfection of the same host, cross- or copackaging may allow distinct retroviruses to generate chimeric variants with unknown pathogenic potential.
[Mh] Termos MeSH primário: Vetores Genéticos
Lentivirus Felinos/genética
Lentivirus de Primatas/genética
RNA Viral
[Mh] Termos MeSH secundário: Animais
Células COS
Capsídeo/metabolismo
Técnicas de Transferência de Genes
HIV-1/genética
HIV-1/crescimento & desenvolvimento
Seres Humanos
Lentivirus Felinos/crescimento & desenvolvimento
Lentivirus de Primatas/crescimento & desenvolvimento
Vírus dos Macacos de Mason-Pfizer/genética
Vírus dos Macacos de Mason-Pfizer/crescimento & desenvolvimento
Homologia de Sequência
Vírus da Imunodeficiência Símia/genética
Vírus da Imunodeficiência Símia/crescimento & desenvolvimento
Especificidade da Espécie
Transformação Genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:0106
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:010503
[St] Status:MEDLINE



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