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  1 / 1529 MEDLINE  
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[PMID]:28934316
[Au] Autor:Miller C; Boegler K; Carver S; MacMillan M; Bielefeldt-Ohmann H; VandeWoude S
[Ad] Endereço:Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado.
[Ti] Título:Pathogenesis of oral FIV infection.
[So] Source:PLoS One;12(9):e0185138, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Feline immunodeficiency virus (FIV) is the feline analogue of human immunodeficiency virus (HIV) and features many hallmarks of HIV infection and pathogenesis, including the development of concurrent oral lesions. While HIV is typically transmitted via parenteral transmucosal contact, recent studies prove that oral transmission can occur, and that saliva from infected individuals contains significant amounts of HIV RNA and DNA. While it is accepted that FIV is primarily transmitted by biting, few studies have evaluated FIV oral infection kinetics and transmission mechanisms over the last 20 years. Modern quantitative analyses applied to natural FIV oral infection could significantly further our understanding of lentiviral oral disease and transmission. We therefore characterized FIV salivary viral kinetics and antibody secretions to more fully document oral viral pathogenesis. Our results demonstrate that: (i) saliva of FIV-infected cats contains infectious virus particles, FIV viral RNA at levels equivalent to circulation, and lower but significant amounts of FIV proviral DNA; (ii) the ratio of FIV RNA to DNA is significantly higher in saliva than in circulation; (iii) FIV viral load in oral lymphoid tissues (tonsil, lymph nodes) is significantly higher than mucosal tissues (buccal mucosa, salivary gland, tongue); (iv) salivary IgG antibodies increase significantly over time in FIV-infected cats, while salivary IgA levels remain static; and, (v) saliva from naïve Specific Pathogen Free cats inhibits FIV growth in vitro. Collectively, these results suggest that oral lymphoid tissues serve as a site for enhanced FIV replication, resulting in accumulation of FIV particles and FIV-infected cells in saliva. Failure to induce a virus-specific oral mucosal antibody response, and/or viral capability to overcome inhibitory components in saliva may perpetuate chronic oral cavity infection. Based upon these findings, we propose a model of oral FIV pathogenesis and suggest alternative diagnostic modalities and translational approaches to study oral HIV infection.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida Felina/etiologia
Vírus da Imunodeficiência Felina/fisiologia
Boca/virologia
Saliva/virologia
[Mh] Termos MeSH secundário: Animais
Especificidade de Anticorpos
Gatos
DNA Viral/sangue
DNA Viral/metabolismo
Síndrome de Imunodeficiência Adquirida Felina/sangue
Síndrome de Imunodeficiência Adquirida Felina/transmissão
Vírus da Imunodeficiência Felina/imunologia
Imunoglobulina A/imunologia
RNA Viral/sangue
RNA Viral/metabolismo
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Immunoglobulin A); 0 (RNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185138


  2 / 1529 MEDLINE  
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[PMID]:28918382
[Au] Autor:Stavisky J; Dean RS; Molloy MH
[Ad] Endereço:Centre for Evidence-based Veterinary Medicine, School of Veterinary Medicine and Science, The University of Nottingham, Loughborough, UK.
[Ti] Título:Prevalence of and risk factors for FIV and FeLV infection in two shelters in the United Kingdom (2011-2012).
[So] Source:Vet Rec;181(17):451, 2017 Oct 28.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aims of this study were to determine the prevalence of feline leukaemia virus (FeLV) and feline immunodeficiency virus (FIV) infections in cats presented to two RSPCA (Royal Society for the Prevention of Cruelty to Animals) animal rehoming centres and to identify risk factors for infection. All cats presented at each centre between August 2011 and August 2012 were subjected to a patient-side test for FeLV/FIV on entry. Kittens under three months and cats euthanased within a short time of presentation were excluded from the study. Univariable and multivariable logistic regression were used to separately determine risk factors for FeLV and FIV infections. At shelter A, the prevalence of FIV infection was 11.4 per cent (54/474) and FeLV infection was 3 per cent (14/473), with two FIV/FeLV coinfections identified. At shelter B, the prevalence of FIV infection was 3 per cent (4/135) and FeLV infection was 0 per cent (0/135). Cats at shelter A were significantly more likely than those at shelter B to test positive for FIV (p=0.0024) and FeLV (p=0.048). Male cats were more likely to be infected with FIV (odds ratio 27.1, p=0.001), and thin body condition and musculoskeletal disease were associated with risk of FeLV. Overall, FIV-positive and FeLV-positive cats were significantly older (median ages 5.1 and 4.75 years, respectively) than the uninfected populations (median ages 3.4 and 3.5 years, respectively). This study shows that the prevalence of these diseases varies between shelter populations. Local knowledge combined with the risk factors identified may be useful in focusing resources for population testing strategies.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Abrigo para Animais/estatística & dados numéricos
Vírus da Imunodeficiência Felina/isolamento & purificação
Vírus da Leucemia Felina/isolamento & purificação
Leucemia Felina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Gatos
Instituições de Caridade
Feminino
Masculino
Prevalência
Fatores de Risco
Reino Unido/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170918
[St] Status:MEDLINE
[do] DOI:10.1136/vr.103857


  3 / 1529 MEDLINE  
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[PMID]:28780918
[Au] Autor:Sato S; Kabeya H; Negishi A; Tsujimoto H; Nishigaki K; Endo Y; Maruyama S
[Ad] Endereço:Laboratory of Veterinary Public Health, Department of Veterinary Medicine,College of Bioresource Sciences, Nihon University,1866 Kameino, Fujisawa, Kanagawa Prefecture, 252-0880,Japan.
[Ti] Título:Molecular survey of Bartonella henselae and Bartonella clarridgeiae in pet cats across Japan by species-specific nested-PCR.
[So] Source:Epidemiol Infect;145(13):2694-2700, 2017 10.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cats are known to be the main reservoir for Bartonella henselae and Bartonella clarridgeiae, which are the agents of 'cat-scratch disease' in humans. In the present study, we investigated the prevalence of the two Bartonella species on 1754 cat bloods collected from all prefectures in Japan during 2007-2008 by a nested-polymerase chain reaction (PCR) targeting the 16S-23S rRNA internal transcribed spacer region. Overall, Bartonella DNA was detected in 4·6% (80/1754) of the cats examined. The nested-PCR showed that 48·8% (39/80) of the positive cats were infected with B. henselae mono-infection, 33·8% (27/80) with B. clarridgeiae mono-infection and 17·5% (14/80) were infected with both species. The prevalence (5·9%; 65/1103) of Bartonella infection in the western part of Japan was significantly higher than that (2·3%; 15/651) of eastern Japan (P < 0·001). Statistical analysis of the cats examined suggested a significant association between Bartonella infection and FeLV infection (OR = 1·9; 95% CI = 1·1-3·4), but not with FIV infection (OR = 1·6; 95% CI = 1·0-2·6).
[Mh] Termos MeSH primário: Bartonella/isolamento & purificação
Doença da Arranhadura de Gato/veterinária
Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Leucemia Felina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Bartonella/classificação
Bartonella/genética
Bartonella henselae/classificação
Bartonella henselae/genética
Bartonella henselae/isolamento & purificação
Doença da Arranhadura de Gato/epidemiologia
Doença da Arranhadura de Gato/microbiologia
Gatos
Síndrome de Imunodeficiência Adquirida Felina/virologia
Feminino
Vírus da Imunodeficiência Felina/isolamento & purificação
Japão/epidemiologia
Vírus da Leucemia Felina/isolamento & purificação
Leucemia Felina/virologia
Masculino
Reação em Cadeia da Polimerase/veterinária
Prevalência
RNA Ribossômico/análise
RNA Viral/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal); 0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE
[do] DOI:10.1017/S0950268817001601


  4 / 1529 MEDLINE  
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[PMID]:28510253
[Au] Autor:Lacerda LC; Silva AN; Freitas JS; Cruz RDS; Said RA; Munhoz AD
[Ad] Endereço:Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
[Ti] Título:Feline immunodeficiency virus and feline leukemia virus: frequency and associated factors in cats in northeastern Brazil.
[So] Source:Genet Mol Res;16(2), 2017 May 10.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Our aims were to determine the frequencies of feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) in owned and stray cats in the northeastern region of Brazil, ascertain the status of FeLV infection, and investigate potential associated factors among the owned cats. Blood samples from 200 asymptomatic owned cats and 30 stray cats were processed using nested PCR and commercial immunochromatographic tests to diagnose infections. To evaluate the factors associated with FIV and/or FeLV in owned cats, a semi-structured interview was conducted with each owner about the animal's environment, and these data were subjected to unconditional logistic regression. The frequencies for owned cats were 6% (12/200) and 3% (6/200) for FIV and FeLV, respectively. No owned cat was positive for both viruses. Stray cats showed frequencies of 6.66% (2/30) and 0% (0/30) for FIV and FeLV, respectively. Contact with other cats and living in peri-urban areas were considered to be risk factors (P < 0.05) for FIV. We did not identify any factors associated with infections with FeLV. Our results confirm the presence of these two retroviruses in the region under study. Our use of different diagnostic techniques allowed us to determine the frequency of retroviruses in the feline population more accurately, particularly with regard to infections by FeLV, which have complex pathogenesis.
[Mh] Termos MeSH primário: Gatos/virologia
Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Vírus da Imunodeficiência Felina/genética
Vírus da Leucemia Felina/genética
Leucemia Felina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Brasil
Síndrome de Imunodeficiência Adquirida Felina/virologia
Feminino
Leucemia Felina/virologia
Masculino
Animais de Estimação/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16029633


  5 / 1529 MEDLINE  
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[PMID]:28475623
[Au] Autor:Ovejero CA; Affranchino JL; González SA
[Ad] Endereço:Laboratorio de Virología, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)-Universidad de Belgrano (UB), Buenos Aires, Argentina.
[Ti] Título:Analysis of the functional compatibility of SIV capsid sequences in the context of the FIV gag precursor.
[So] Source:PLoS One;12(5):e0177297, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The formation of immature lentiviral particles is dependent on the multimerization of the Gag polyprotein at the plasma membrane of the infected cells. One key player in the virus assembly process is the capsid (CA) domain of Gag, which establishes the protein-protein interactions that give rise to the hexagonal lattice of Gag molecules in the immature virion. To gain a better understanding of the functional equivalence between the CA proteins of simian and feline immunodeficiency viruses (SIV and FIV, respectively), we generated a series of chimeric FIV Gag proteins in which the CA-coding region was partially or totally replaced by its SIV counterpart. All the FIV Gag chimeras were found to be assembly-defective; however, all of them are able to interact with wild-type SIV Gag and be recruited into extracellular virus-like particles, regardless of the SIV CA sequences present in the chimeric FIV Gag. The results presented here markedly contrast with our previous findings showing that chimeric SIVs carrying FIV CA-derived sequences are assembly-competent. Overall, our data support the notion that although the SIV and FIV CA proteins share 51% amino acid sequence similarity and exhibit a similar organization, i.e., an N-terminal domain joined by a flexible linker to a C-terminal domain, their functional exchange between these different lentiviruses is strictly dependent on the context of the recipient Gag precursor.
[Mh] Termos MeSH primário: Capsídeo/metabolismo
Produtos do Gene gag/metabolismo
Vírus da Imunodeficiência Felina/metabolismo
Vírus da Imunodeficiência Símia/metabolismo
[Mh] Termos MeSH secundário: Animais
Células COS
Proteínas do Capsídeo/metabolismo
Cercopithecus aethiops
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins); 0 (Gene Products, gag)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177297


  6 / 1529 MEDLINE  
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[PMID]:28384338
[Au] Autor:Eckstrand CD; Sparger EE; Pitt KA; Murphy BG
[Ad] Endereço:Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, United States of America.
[Ti] Título:Peripheral and central immune cell reservoirs in tissues from asymptomatic cats chronically infected with feline immunodeficiency virus.
[So] Source:PLoS One;12(4):e0175327, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Feline immunodeficiency virus (FIV) infection in cats results in life-long viral persistence and progressive immunopathology. We have previously described a cohort of experimentally infected cats demonstrating a progressive decline of peripheral blood CD4+ T-cell over six years in the face of apparent peripheral viral latency. More recently we reported findings from this same cohort that revealed popliteal lymph node tissue as sites for ongoing viral replication suggesting that tissue reservoirs are important in FIV immunopathogenesis during the late asymptomatic phase of infection. Results reported herein characterize important tissue reservoirs of active viral replication during the late asymptomatic phase by examining biopsied specimens of spleen, mesenteric lymph node (MLN), and intestine from FIV-infected and uninfected control cats. Peripheral blood collected coincident with harvest of tissues demonstrated severe CD4+ T-cell depletion, undetectable plasma viral gag RNA and rarely detectable peripheral blood mononuclear cell (PBMC)-associated viral RNA (vRNA) by real-time PCR. However, vRNA was detectable in all three tissue sites from three of four FIV-infected cats despite the absence of detectable vRNA in plasma. A novel in situ hybridization assay identified B cell lymphoid follicular domains as microanatomical foci of ongoing FIV replication. Additionally, we demonstrated that CD4+ leukocyte depletion in tissues, and CD4+ and CD21+ leukocytes as important cellular reservoirs of ongoing replication. These findings revealed that tissue reservoirs support foci of ongoing viral replication, in spite of highly restricted viral replication in blood. Lentiviral eradication strategies will need address tissue viral reservoirs.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida Felina/imunologia
[Mh] Termos MeSH secundário: Animais
Linfócitos T CD4-Positivos/imunologia
Gatos
Doença Crônica
Progressão da Doença
Vírus da Imunodeficiência Felina/genética
Vírus da Imunodeficiência Felina/fisiologia
Depleção Linfocítica
Tecido Linfoide/imunologia
Tecido Linfoide/virologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175327


  7 / 1529 MEDLINE  
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[PMID]:28331087
[Au] Autor:Yoshikawa R; Takeuchi JS; Yamada E; Nakano Y; Misawa N; Kimura Y; Ren F; Miyazawa T; Koyanagi Y; Sato K
[Ad] Endereço:Laboratory of Viral Pathogenesis, Institute for Virus Research, Kyoto University, Kyoto, Japan.
[Ti] Título:Feline Immunodeficiency Virus Evolutionarily Acquires Two Proteins, Vif and Protease, Capable of Antagonizing Feline APOBEC3.
[So] Source:J Virol;91(11), 2017 Jun 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The interplay between viral and host proteins has been well studied to elucidate virus-host interactions and their relevance to virulence. Mammalian genes encode apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3 (APOBEC3) proteins, which act as intrinsic restriction factors against lentiviruses. To overcome APOBEC3-mediated antiviral actions, lentiviruses have evolutionarily acquired an accessory protein, viral infectivity factor (Vif), and Vif degrades host APOBEC3 proteins via a ubiquitin/proteasome-dependent pathway. Although the Vif-APOBEC3 interaction and its evolutionary significance, particularly those of primate lentiviruses (including HIV) and primates (including humans), have been well investigated, those of nonprimate lentiviruses and nonprimates are poorly understood. Moreover, the factors that determine lentiviral pathogenicity remain unclear. Here, we focus on feline immunodeficiency virus (FIV), a pathogenic lentivirus in domestic cats, and the interaction between FIV Vif and feline APOBEC3 in terms of viral virulence and evolution. We reveal the significantly reduced diversity of FIV subtype B compared to that of other subtypes, which may associate with the low pathogenicity of this subtype. We also demonstrate that FIV subtype B Vif is less active with regard to feline APOBEC3 degradation. More intriguingly, we further reveal that FIV protease cleaves feline APOBEC3 in released virions. Taken together, our findings provide evidence that a lentivirus encodes two types of anti-APOBEC3 factors, Vif and viral protease. During the history of mammalian evolution, mammals coevolved with retroviruses, including lentiviruses. All pathogenic lentiviruses, excluding equine infectious anemia virus, have acquired the gene via evolution to combat APOBEC3 proteins, which are intrinsic restriction factors against exogenous lentiviruses. Here we demonstrate that FIV, a pathogenic lentivirus in domestic cats, antagonizes feline APOBEC3 proteins by both Vif and a viral protease. Furthermore, the Vif proteins of an FIV subtype (subtype B) have attenuated their anti-APOBEC3 activity through evolution. Our findings can be a clue to elucidate the complicated evolutionary processes by which lentiviruses adapt to mammals.
[Mh] Termos MeSH primário: Desaminases APOBEC/antagonistas & inibidores
Ácido Aspártico Endopeptidases/metabolismo
Produtos do Gene vif/metabolismo
Vírus da Imunodeficiência Felina/genética
[Mh] Termos MeSH secundário: Desaminases APOBEC/metabolismo
Animais
Ácido Aspártico Endopeptidases/genética
Gatos
Evolução Molecular
Produtos do Gene vif/genética
Interações Hospedeiro-Patógeno
Vírus da Imunodeficiência Felina/metabolismo
Vírus da Imunodeficiência Felina/patogenicidade
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Gene Products, vif); EC 3.4.23.- (Aspartic Acid Endopeptidases); EC 3.4.23.- (FIV protease); EC 3.5.4.5 (APOBEC Deaminases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170727
[Lr] Data última revisão:
170727
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE


  8 / 1529 MEDLINE  
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[PMID]:28131385
[Au] Autor:Westman ME; Malik R; Hall E; Sheehy PA; Norris JM
[Ad] Endereço:Sydney School of Veterinary Science, The University of Sydney, Sydney, NSW 2006, Australia. Electronic address: mark.westman@sydney.edu.au.
[Ti] Título:Comparison of three feline leukaemia virus (FeLV) point-of-care antigen test kits using blood and saliva.
[So] Source:Comp Immunol Microbiol Infect Dis;50:88-96, 2017 Feb.
[Is] ISSN:1878-1667
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Feline leukaemia virus (FeLV) can be a challenging infection to diagnose due to a complex feline host-pathogen relationship and occasionally unreliable test results. This study compared the accuracy of three point-of-care (PoC) FeLV p27 antigen test kits commonly used in Australia and available commercially worldwide (SNAP FIV/FeLV Combo, Witness FeLV/FIV and Anigen Rapid FIV/FeLV), using detection of FeLV provirus by an in-house real-time polymerase chain reaction (qPCR) assay as the diagnostic gold standard. Blood (n=563) and saliva (n=419) specimens were collected from a population of cats determined to include 491 FeLV-uninfected and 72 FeLV-infected individuals (45 progressive infections [p27 and qPCR positive], 27 regressive infections [p27 negative, qPCR positive]). Sensitivity and specificity using whole blood was 63% and 94% for SNAP Combo, 57% and 98% for Witness, and 57% and 98% for Anigen Rapid, respectively. SNAP Combo had a significantly lower specificity using blood compared to the other two kits (P=0.004 compared to Witness, P=0.007 compared to Anigen Rapid). False-positive test results occurred with all three kits using blood, and although using any two kits in parallel increased specificity, no combination of kits completely eliminated the occurrence of false-positive results. We therefore recommend FeLV proviral PCR testing for any cat that tests positive with a PoC FeLV antigen kit, as well as for any cat that has been potentially exposed to FeLV but tests negative with a FeLV antigen kit, before final assignment of FeLV status can be made with confidence. For saliva testing, sensitivity and specificity was 54% and 100%, respectively, for all three test kits. The reduced sensitivity of saliva testing compared to blood testing, although not statistically significant, suggests saliva testing with the current generation of PoC FeLV antigen kits is unsuitable for screening large populations of cats, such as in shelters.
[Mh] Termos MeSH primário: Antígenos Virais/análise
Doenças do Gato/diagnóstico
Ensaio de Imunoadsorção Enzimática/veterinária
Vírus da Leucemia Felina/isolamento & purificação
Infecções por Retroviridae/veterinária
Saliva/virologia
Infecções Tumorais por Vírus/veterinária
[Mh] Termos MeSH secundário: Animais
Austrália
Doenças do Gato/virologia
Gatos/virologia
Vírus da Imunodeficiência Felina/imunologia
Vírus da Leucemia Felina/imunologia
Testes Imediatos
Kit de Reagentes para Diagnóstico/veterinária
Reação em Cadeia da Polimerase em Tempo Real
Infecções por Retroviridae/diagnóstico
Infecções por Retroviridae/virologia
Sensibilidade e Especificidade
Organismos Livres de Patógenos Específicos
Infecções Tumorais por Vírus/diagnóstico
Infecções Tumorais por Vírus/virologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Viral); 0 (Reagent Kits, Diagnostic)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170217
[Lr] Data última revisão:
170217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170130
[St] Status:MEDLINE


  9 / 1529 MEDLINE  
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[PMID]:28107367
[Au] Autor:Zhang J; Wang L; Li J; Kelly P; Price S; Wang C
[Ad] Endereço:Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu, China.
[Ti] Título:First Molecular Characterization of Feline Immunodeficiency Virus in Domestic Cats from Mainland China.
[So] Source:PLoS One;12(1):e0169739, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The feline immunodeficiency virus (FIV) is a retrovirus of the Lentivirus genus that was initially isolated from a colony of domestic cats in California in 1986 and has now been recognized as a common feline pathogen worldwide. To date, there is only one recent serology-based report on FIV in mainland China which was published in 2016. We designed this study to investigate the molecular prevalence and diversity of feline immunodeficiency virus (FIV) in domestic cats from mainland China. We studied the prevalence of FIV in whole blood samples of 615 domestic cats in five cities (Beijing, Guangzhou, Nanjing, Shanghai and Yangzhou) of mainland China and examined them using FRET-PCR (Fluorescence Resonance Energy Transfer-Polymerase Chain Reaction) and regular PCRs for the gag and env genes. Overall, 1.3% (8/615) of the cats were positive for provirus DNA with nucleotide analysis using PCRs for the gag and env sequences showing the cats were infected with FIV subtype A. This is the first molecular characterization of FIV in mainland China and the first description of subtype A in continental Asia.
[Mh] Termos MeSH primário: Vírus da Imunodeficiência Felina/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Animais Domésticos
Gatos
China
Transferência Ressonante de Energia de Fluorescência
Genes Virais
Vírus da Imunodeficiência Felina/classificação
Filogenia
Reação em Cadeia da Polimerase
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170810
[Lr] Data última revisão:
170810
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0169739


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[PMID]:28003486
[Au] Autor:Lee J; Malmberg JL; Wood BA; Hladky S; Troyer R; Roelke M; Cunningham M; McBride R; Vickers W; Boyce W; Boydston E; Serieys L; Riley S; Crooks K; VandeWoude S
[Ad] Endereço:Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, USA.
[Ti] Título:Feline Immunodeficiency Virus Cross-Species Transmission: Implications for Emergence of New Lentiviral Infections.
[So] Source:J Virol;91(5), 2017 Mar 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Owing to a complex history of host-parasite coevolution, lentiviruses exhibit a high degree of species specificity. Given the well-documented viral archeology of human immunodeficiency virus (HIV) emergence following human exposures to simian immunodeficiency virus (SIV), an understanding of processes that promote successful cross-species lentiviral transmissions is highly relevant. We previously reported natural cross-species transmission of a subtype of feline immunodeficiency virus, puma lentivirus A (PLVA), between bobcats ( ) and mountain lions ( ) for a small number of animals in California and Florida. In this study, we investigate host-specific selection pressures, within-host viral fitness, and inter- versus intraspecies transmission patterns among a larger collection of PLV isolates from free-ranging bobcats and mountain lions. Analyses of proviral and viral RNA levels demonstrate that PLVA fitness is severely restricted in mountain lions compared to that in bobcats. We document evidence of diversifying selection in three of six PLVA genomes from mountain lions, but we did not detect selection among 20 PLVA isolates from bobcats. These findings support the hypothesis that PLVA is a bobcat-adapted virus which is less fit in mountain lions and under intense selection pressure in the novel host. Ancestral reconstruction of transmission events reveals that intraspecific PLVA transmission has occurred among panthers ( ) in Florida following the initial cross-species infection from bobcats. In contrast, interspecific transmission from bobcats to mountain lions predominates in California. These findings document outcomes of cross-species lentiviral transmission events among felids that compare to the emergence of HIV from nonhuman primates. Cross-species transmission episodes can be singular, dead-end events or can result in viral replication and spread in the new species. The factors that determine which outcome will occur are complex, and the risk of new virus emergence is therefore difficult to predict. We used molecular techniques to evaluate the transmission, fitness, and adaptation of puma lentivirus A (PLVA) between bobcats and mountain lions in two geographic regions. Our findings illustrate that mountain lion exposure to PLVA is relatively common but does not routinely result in communicable infections in the new host. This is attributed to efficient species barriers that largely prevent lentiviral adaptation. However, the evolutionary capacity for lentiviruses to adapt to novel environments may ultimately overcome host restriction mechanisms over time and under certain ecological circumstances. This phenomenon provides a unique opportunity to examine cross-species transmission events leading to new lentiviral emergence.
[Mh] Termos MeSH primário: Doenças do Gato/virologia
Vírus da Imunodeficiência Felina/fisiologia
Lynx/virologia
Puma/virologia
[Mh] Termos MeSH secundário: Animais
California/epidemiologia
Doenças do Gato/epidemiologia
Doenças do Gato/transmissão
Gatos
Feminino
Florida/epidemiologia
Masculino
Filogenia
Polimorfismo Genético
Seleção Genética
Especificidade da Espécie
Tropismo Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161223
[St] Status:MEDLINE



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