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[PMID]:28408784
[Au] Autor:Stonos N; Bauman C; Menzies P; Wootton SK; Karrow NA
[Ad] Endereço:Centre for the Genetic Improvement of Livestock, Department of Animal Bioscience (Stonos, Karrow); Department of Population Medicine (Bauman, Menzies); Department of Pathobiology, University of Guelph, Guelph, Ontario (Wootton).
[Ti] Título:Prevalence of small ruminant lentivirus and subsp. co-infection in Ontario dairy sheep and dairy goats.
[So] Source:Can J Vet Res;81(2):155-159, 2017 Apr.
[Is] ISSN:1928-9022
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:Infection with small ruminant lentiviruses (SRLV) causes a variety of chronic inflammatory conditions that limit production. subsp. (MAP) is also a major production-limiting disease of sheep and goats, which causes severe inflammation of the small intestine. Previous studies have indicated that both SRLV and MAP are widespread in small ruminants in Ontario. This study estimated the prevalence of SRLV and MAP co-infection. Serum samples that were previously tested for MAP infection were re-tested for SRLV. The apparent prevalence of co-infection was low, with 3.4% [95% confidence interval (CI): 1.9 to 5.9] and 14.3% (95% CI: 11.6 to 17.5) of sheep and goats respectively, positive for both infections. However, co-infection is widespread with 36.8% (95% CI: 19.1 to 59.1) and 71.4% (95% CI: 52.8 to 84.9) of sheep and goat farms with 1 or more co-infected animals. A significant association was found between SRLV seropositivity and MAP fecal culture ( = 0.021), suggesting that co-infected goats may be more likely to shed MAP in their feces.
[Mh] Termos MeSH primário: Doenças das Cabras/epidemiologia
Infecções por Lentivirus/veterinária
Lentivirus Ovinos-Caprinos/isolamento & purificação
Mycobacterium avium subsp. paratuberculosis/isolamento & purificação
Paratuberculose/complicações
Doenças dos Ovinos/epidemiologia
[Mh] Termos MeSH secundário: Animais
Estudos Transversais
Doenças das Cabras/microbiologia
Doenças das Cabras/virologia
Cabras
Infecções por Lentivirus/complicações
Infecções por Lentivirus/epidemiologia
Infecções por Lentivirus/virologia
Ontário/epidemiologia
Paratuberculose/diagnóstico
Paratuberculose/epidemiologia
Ovinos
Doenças dos Ovinos/microbiologia
Doenças dos Ovinos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE


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[PMID]:28113037
[Au] Autor:Pinczowski P; Sanjosé L; Gimeno M; Crespo H; Glaria I; Amorena B; de Andrés D; Pérez M; Reina R; Luján L
[Ad] Endereço:1 Department of Animal Pathology, University of Zaragoza, Spain.
[Ti] Título:Small Ruminant Lentiviruses in Sheep: Pathology and Tropism of 2 Strains Using the Bone Marrow Route.
[So] Source:Vet Pathol;54(3):413-424, 2017 May.
[Is] ISSN:1544-2217
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of this work was to comparatively study the tissue tropism and the associated pathology of 2 autochthonous small ruminant lentivirus (SRLV) field strains using an experimental infection in sheep through the bone marrow. Fifteen male, SRLV-free lambs of the Rasa Aragonesa breed were inoculated with strain 697 (nervous tissue origin, animals A1-A6), with strain 496 (articular origin, animals B1-B6), or with uninfected culture medium (C1-C3). Clinical, serologic, and polymerase chain reaction (PCR) evaluations were performed periodically. Two lambs from each infected group and a control animal were euthanized at 134, 273, and 319 days postinfection. Tissues were analyzed by gross and histopathologic evaluation; immunohistochemistry for CD3, CD4, CD8, CD68, and FoxP3 cell markers; lung morphometric evaluation; and tissue proviral quantification by PCR. All infected animals became positive either by enzyme-linked immunosorbent assay and/or PCR, with group B lambs showing the highest serologic values and more consistently positive PCR reactions. Group A lambs showed representative lung lesions but only mild histopathologic changes in the central nervous system (CNS) or in carpal joints. Contrarily, group B lambs demonstrated intense carpal arthritis and interstitial pneumonia but an absence of lesions in the CNS. Proviral copies in tissues were detected only in group B lambs. Experimental infection with these SRLV strains indicates that strain 496 is more virulent than strain 697 and more prone to induce arthritis, whereas strain 697 is more likely to reproduce encephalitis in Rasa Aragonesa lambs. Host factors as well as viral factors are responsible for the final clinicopathologic picture during SRLV infections.
[Mh] Termos MeSH primário: Medula Óssea/virologia
Infecções por Lentivirus/veterinária
Lentivirus Ovinos-Caprinos/patogenicidade
Tropismo Viral
[Mh] Termos MeSH secundário: Animais
Medula Óssea/patologia
Sistema Nervoso Central/patologia
Sistema Nervoso Central/virologia
Ensaio de Imunoadsorção Enzimática/veterinária
Articulações/patologia
Articulações/virologia
Infecções por Lentivirus/patologia
Infecções por Lentivirus/virologia
Pulmão/patologia
Pulmão/virologia
Masculino
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Ovinos/virologia
Tropismo Viral/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1177/0300985816688742


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[PMID]:27461580
[Au] Autor:Sánchez JH; Martínez HA; García MM; Garrido G; Gómez L; Aguilar JA; de Andrés DF; Reina R; Ramírez H
[Ad] Endereço:Laboratorio de Virología, Genética y Biología Molecular, Facultad de Estudios Superiores Cuautitlán (FESC) Campo 4, Universidad Nacional Autónoma de México (UNAM), Estado de México, México.
[Ti] Título:The presence of small ruminant lentiviruses in Mexican Pelibuey sheep.
[So] Source:Theriogenology;86(8):1953-1957.e1, 2016 Nov.
[Is] ISSN:1879-3231
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The transmission frequency of small ruminant lentiviruses (SRLVs) through the placenta is controversial and may be associated with breed susceptibility. In Mexico, SRLV infections in sheep have been poorly studied. This work explores the presence of antibodies and proviral DNA in Mexican Pelibuey sheep. Enzyme-linked immunosorbent assays (ELISAs; three commercial kits and two on the basis of synthetic peptides) and polymerase chain reaction (PCR; amplifying the long terminal repeat and gag segments) were performed to diagnose SRLV infection in 25 adult Pelibuey ewes with an average age of 2.5 years and 32 fetuses with gestational ages ranging from 40 to 90 days without clinical signs of SRLV. Two of the three commercial ELISAs and the synthetic peptide-based ones were positive for SRLV antibody detection in 28% and 24% of the ewes, respectively, whereas none of the fetuses were positive by any of the ELISAs. By PCR, 31% of the ewes and, interestingly, two fetuses were positive. Characteristic SRLV lesions were not found in the fetal and/or ewe tissues, including those with positive PCR results. These findings demonstrate the susceptibility of Pelibuey sheep to SRLV infection and the low transmission frequency through the placenta.
[Mh] Termos MeSH primário: Infecções por Lentivirus/veterinária
Lentivirus Ovinos-Caprinos/isolamento & purificação
Doenças dos Ovinos/virologia
[Mh] Termos MeSH secundário: Animais
Ensaio de Imunoadsorção Enzimática/veterinária
Feminino
Infecções por Lentivirus/epidemiologia
Infecções por Lentivirus/virologia
Lentivirus Ovinos-Caprinos/classificação
México/epidemiologia
Ovinos
Doenças dos Ovinos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160728
[St] Status:MEDLINE


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[PMID]:25462356
[Au] Autor:L'Homme Y; Leboeuf A; Arsenault J; Fras M
[Ad] Endereço:Canadian Food Inspection Agency, St-Hyacinthe Laboratory, 3400 Blvd Casavant West, St-Hyacinthe, Quebec, Canada J2S 8E3; Faculty of Veterinary Medicine, Université de Montréal, 3200 Sicotte, St-Hyacinthe, Quebec, Canada J2S 7C6. Electronic address: yvan.lhomme@umontreal.ca.
[Ti] Título:Identification and characterization of an emerging small ruminant lentivirus circulating recombinant form (CRF).
[So] Source:Virology;475:159-71, 2015 Jan 15.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The molecular epidemiology of small ruminant lentiviruses (SRLVs) is constantly changing due to animal movements, cross species transmission and because of their rapid evolutionary rate. This study reports a comprehensive genetic and phylogenetic analysis based on consensus gag and pol sequences covering 3kb of the SRLV genome from small ruminants in Québec, Canada. A group of strains obtained from goats originating from different flocks, segregated in a unique clade distinct from currently known SRLV groups. Genetic dissection of the gag gene from these strains revealed that it originated as a result of a recombination event between parental strains currently circulating in small ruminants of the country. Following HIV nomenclature, we propose to call this group of strains, circulating recombinant form 1 SRLV, or CRF01_AB SRLV. In addition, the study confirms the existence of genetically distinct and homogeneous populations of SRLVs infecting sheep and goats housed in single species flocks.
[Mh] Termos MeSH primário: Doenças das Cabras/virologia
Infecções por Lentivirus/veterinária
Lentivirus Ovinos-Caprinos/genética
Lentivirus Ovinos-Caprinos/isolamento & purificação
Doenças dos Ovinos/virologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
DNA Viral/genética
Proteínas de Fusão gag-pol/química
Proteínas de Fusão gag-pol/genética
Variação Genética
Doenças das Cabras/epidemiologia
Cabras
Infecções por Lentivirus/epidemiologia
Infecções por Lentivirus/virologia
Lentivirus Ovinos-Caprinos/classificação
Dados de Sequência Molecular
Filogenia
Quebeque/epidemiologia
Alinhamento de Sequência
Testes Sorológicos
Ovinos
Doenças dos Ovinos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Fusion Proteins, gag-pol)
[Em] Mês de entrada:1503
[Cu] Atualização por classe:141229
[Lr] Data última revisão:
141229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


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[PMID]:25286660
[Au] Autor:Nowicka D; Czopowicz M; Mickiewicz M; Szalus-Jordanow O; Witkowski L; Bagnicka E; Kaba J
[Ti] Título:Diagnostic performance of ID screen MVV-CAEV Indirect Screening ELISA in identifying small ruminant lentiviruses-infected goats.
[So] Source:Pol J Vet Sci;17(3):501-6, 2014.
[Is] ISSN:1505-1773
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Diagnostic performance of ID Screen MVV-CAEV Indirect Screening ELISA in identifying goats infected with small ruminant lentiviruses (SRLV) was evaluated. In total 299 serum samples from the collection of the Laboratory of Veterinary Epidemiology and Economics--109 truly positive and 190 truly negative--were used. To be enrolled in the study a serum sample had to come from at least 2 year-old goat which had reacted identically in two serological surveys preceding sample collection and was kept in a herd of stable serological status confirmed at least twice during preceding 5 years. Moreover, in seropositive herds at least 20% of goats had to be serologically positive at the moment when the serum sample was collected for the study. The test proved to have high accuracy. Area under curve was 98.8% (95% CI: 97.5%, 100%). Diagnostic performance of the test was almost identical (Youlden's index of 90%, sensitivity > 90% and specificity > 95%) within a fairly wide range of cut-off values--between 20% and 60%. At manufacturer's cut-off of 50% sensitivity and specificity were 91.7% (95% CI: 85.0%, 95.6%) and 98.9% (95% CI: 96.2%, 99.7%), respectively. For this cut-off positive likelihood ratio was 87 (95% CI: 22, 346) and negative likelihood ratio was 0.08 (95% CI: 0.04, 0.16). In conclusion, the results of this study indicate that ID Screen MVV-CAEV Indirect Screening ELISA is a highly accurate diagnostic test for SRLV infection.
[Mh] Termos MeSH primário: Ensaio de Imunoadsorção Enzimática/veterinária
Doenças das Cabras/virologia
Infecções por Lentivirus/veterinária
Lentivirus Ovinos-Caprinos
[Mh] Termos MeSH secundário: Animais
Ensaio de Imunoadsorção Enzimática/métodos
Doenças das Cabras/diagnóstico
Cabras
Infecções por Lentivirus/diagnóstico
Infecções por Lentivirus/virologia
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1411
[Cu] Atualização por classe:141007
[Lr] Data última revisão:
141007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141008
[St] Status:MEDLINE


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[PMID]:24303974
[Au] Autor:White SN; Mousel MR; Reynolds JO; Herrmann-Hoesing LM; Knowles DP
[Ad] Endereço:Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, WA, 99164, USA; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, 99164, USA.
[Ti] Título:Deletion variant near ZNF389 is associated with control of ovine lentivirus in multiple sheep flocks.
[So] Source:Anim Genet;45(2):297-300, 2014 Apr.
[Is] ISSN:1365-2052
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ovine lentivirus (OvLV) is a macrophage-tropic lentivirus found in many countries that causes interstitial pneumonia, mastitis, arthritis and cachexia in sheep. There is no preventive vaccine and no cure, but breed differences suggest marker-assisted selective breeding might improve odds of infection and control of OvLV post-infection. Although variants in TMEM154 have consistent association with odds of infection, no variant in any gene has been associated with host control of OvLV post-infection in multiple animal sets. Proviral concentration is a live-animal diagnostic measure of OvLV control post-infection related to severity of OvLV-induced lesions. A recent genome-wide association study identified a region including four zinc finger genes associated with proviral concentration in one Rambouillet flock. To refine this region, we tested additional variants and identified a small insertion/deletion variant near ZNF389 that showed consistent association with proviral concentration in three animal sets (P < 0.05). These animal sets contained Rambouillet, Polypay and crossbred sheep from multiple locations and management conditions. Strikingly, one flock had exceptionally high prevalence (>87%, including yearlings) and mean proviral concentration (>950 copies/µg), possibly due to needle sharing. The best estimate of proviral concentration by genotype, obtained from all 1310 OvLV-positive animals tested, showed insertion homozygotes had less than half the proviral concentration of other genotypes (P < 0.0001). Future work will test additional breeds, management conditions and viral subtypes, and identify functional properties of the haplotype this deletion variant tracks. To our knowledge, this is the first genetic variant consistently associated with host control of OvLV post-infection in multiple sheep flocks.
[Mh] Termos MeSH primário: Resistência à Doença/genética
Infecções por Lentivirus/veterinária
Deleção de Sequência
Doenças dos Ovinos/genética
[Mh] Termos MeSH secundário: Animais
Genótipo
Infecções por Lentivirus/genética
Infecções por Lentivirus/imunologia
Lentivirus Ovinos-Caprinos/imunologia
Ovinos
Doenças dos Ovinos/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1411
[Cu] Atualização por classe:150422
[Lr] Data última revisão:
150422
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131206
[St] Status:MEDLINE
[do] DOI:10.1111/age.12107


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[PMID]:24004524
[Au] Autor:Kuhar U; Barlic-Maganja D; Grom J
[Ad] Endereço:Veterinary Faculty, Institute for Microbiology and Parasitology, Virology Unit, University of Ljubljana, Gerbiceva 60, SI-1115 Ljubljana, Slovenia. Urska.Kuhar@vf.uni-lj.si.
[Ti] Título:Development and validation of TaqMan probe based real time PCR assays for the specific detection of genotype A and B small ruminant lentivirus strains.
[So] Source:BMC Vet Res;9:172, 2013 Sep 03.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Small ruminant lentiviruses (SRLV) are members of the Retroviridae family and infect goats and sheep worldwide. Detection of specific antibodies using AGID and ELISA is the most commonly used means of diagnosing SRLV infection. The most frequent molecular method for detecting the provirus genome is PCR, using peripheral blood leucocytes as target cells. Real time PCR has also recently been used. The aim of this study was to develop a real time PCR for detection of SRLV in order to improve molecular diagnostics of SRLV infections in sheep and goats. RESULTS: Two new real time PCR assays using TaqMan probes for the specific detection of genotype A (MVV assay) and genoptype B (CAEV assay) SRLV strains and differentiation between them were developed and validated at both analytical and diagnostic levels following MIQE guidelines. The validation results showed that the new real time PCR is 100% specific, with a reliable limit of detection of 26 (CAEV assay) and 72 (MVV assay) plasmid DNA copies, while compared to ELISA the diagnostic sensitivity of both assays was 79% when tested with Slovenian SRLV field samples. Intra-assay and inter-assay coefficients of variation showed overall good repeatability and reproducibility of the new real time PCR assays, except for the highest dilutions. CONCLUSIONS: Two new TaqMan probe based real time PCR assays for the specific detection of genotype A and B SRLV strains and differentiation between them were developed and validated. They can serve as an additional tool for confirming infection with SRLV and may also be useful for early detection of infected animals prior to seroconversion.
[Mh] Termos MeSH primário: Genótipo
Lentivirus Ovinos-Caprinos/genética
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Ruminantes
[Mh] Termos MeSH secundário: Animais
Doenças das Cabras/diagnóstico
Doenças das Cabras/virologia
Cabras
Infecções por Lentivirus/diagnóstico
Infecções por Lentivirus/veterinária
Infecções por Lentivirus/virologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
Ovinos
Doenças dos Ovinos/diagnóstico
Doenças dos Ovinos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1406
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130906
[St] Status:MEDLINE
[do] DOI:10.1186/1746-6148-9-172


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[PMID]:23881276
[Au] Autor:Minardi da Cruz JC; Singh DK; Lamara A; Chebloune Y
[Ad] Endereço:Laboratory Animal Health, Embrapa Goat and Sheep Research Center, CP 145, CEP 62010-970, Sobral, Ceará, Brazil.
[Ti] Título:Small ruminant lentiviruses (SRLVs) break the species barrier to acquire new host range.
[So] Source:Viruses;5(7):1867-84, 2013 Jul 23.
[Is] ISSN:1999-4915
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Zoonotic events of simian immunodeficiency virus (SIV) from non-human primates to humans have generated the acquired immunodeficiency syndrome (AIDS), one of the most devastating infectious disease of the last century with more than 30 million people dead and about 40.3 million people currently infected worldwide. Human immunodeficiency virus (HIV-1 and HIV-2), the two major viruses that cause AIDS in humans are retroviruses of the lentivirus genus. The genus includes arthritis-encephalitis virus (CAEV) and Maedi-Visna virus (MVV), and a heterogeneous group of viruses known as small ruminant lentiviruses (SRLVs), affecting goat and sheep. Lentivirus genome integrates into the host DNA, causing persistent infection associated with a remarkable diversity during viral replication. Direct evidence of mixed infections with these two closely related SRLVs was found in both sheep and goats. The evidence of a genetic continuum with caprine and ovine field isolates demonstrates the absence of an efficient species barrier preventing cross-species transmission. In dual-infected animals, persistent infections with both CAEV and MVV have been described, and viral chimeras have been detected. This not only complicates animal trade between countries but favors the risk that highly pathogenic variants may emerge as has already been observed in the past in Iceland and, more recently, in outbreaks with virulent strains in Spain. SRLVs affecting wildlife have already been identified, demonstrating the existence of emergent viruses adapted to new hosts. Viruses adapted to wildlife ruminants may acquire novel biopathological properties which may endanger not only the new host species but also domestic ruminants and humans. SRLVs infecting sheep and goats follow a genomic evolution similar to that observed in HIV or in other lentiviruses. Lentivirus genetic diversity and host factors leading to the establishment of naturally occurring virulent versus avirulent infections, in addition to the emergence of new strains, challenge every aspect of SRLV control measures for providing efficient tools to prevent the transmission of diseases between wild ungulates and livestock.
[Mh] Termos MeSH primário: Especificidade de Hospedeiro
Lentivirus Ovinos-Caprinos/fisiologia
[Mh] Termos MeSH secundário: Adaptação Biológica
Animais
Vírus da Artrite-Encefalite Caprina/genética
Surtos de Doenças
Cabras
Islândia/epidemiologia
Infecções por Lentivirus/veterinária
Infecções por Lentivirus/virologia
Lentivirus Ovinos-Caprinos/genética
Recombinação Genética
Ovinos
Espanha/epidemiologia
Vírus Visna-Maedi/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Em] Mês de entrada:1401
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130725
[St] Status:MEDLINE
[do] DOI:10.3390/v5071867


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[PMID]:23691111
[Au] Autor:Sarafidou T; Stamatis C; Kalozoumi G; Spyrou V; Fthenakis GC; Billinis C; Mamuris Z
[Ad] Endereço:Department of Biochemistry and Biotechnology, University of Thessaly, Larissa, Greece.
[Ti] Título:Toll like receptor 9 (TLR9) polymorphism G520R in sheep is associated with seropositivity for Small Ruminant Lentivirus.
[So] Source:PLoS One;8(5):e63901, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Infectious diseases of sheep are of major economic importance causing direct and indirect losses. Among the major sheep infectious agents are Small Ruminant Lentivirus, Chlamydophila abortus and Mycobacterium avium subsp. paratuberculosis infections, mainly due to their worldwide distribution and economic impact that they cause. Based on the differential susceptibility to infectious diseases between and within breeds and on the recent findings regarding the putative involvement of TLR9 in disease susceptibility, the aim of this study was to evaluate the levels of nucleotide variation of TLR9 and its mediator MyD88 in three sheep flocks originated from different breeds and assess their possible association with seropositivity/seronegativity for different infectious agents. The analysis indicated that the change of G to R at codon 520 of TLR9 polypeptide shows a significant association with Small Ruminant Lentivirus seropositivity. This amino-acid substitution, which can result in polarity change, might influence structure and function of LRR17, interfering with ligand binding and thus could be used in studies investigating susceptibility/resistance to Small Ruminant Lentivirus infections in sheep.
[Mh] Termos MeSH primário: Lentivirus Ovinos-Caprinos/isolamento & purificação
Polimorfismo de Nucleotídeo Único
Ovinos/genética
Receptor Toll-Like 9/genética
[Mh] Termos MeSH secundário: Animais
Frequência do Gene
Heterozigoto
Dados de Sequência Molecular
Fator 88 de Diferenciação Mieloide/genética
Ovinos/classificação
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Myeloid Differentiation Factor 88); 0 (Toll-Like Receptor 9)
[Em] Mês de entrada:1401
[Cu] Atualização por classe:150426
[Lr] Data última revisão:
150426
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130522
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0063901


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[PMID]:23384458
[Au] Autor:Barquero N; Domenech A; Arjona A; Fernández-Garayzabal JF; Ruiz-Santa-Quiteria JA; Gomez-Lucia E
[Ad] Endereço:Department of Animal Health, Veterinary Science School, Complutense University of Madrid, 28040 Madrid, Spain. nuria.barquero@vet.ucm.es
[Ti] Título:Comparison of two PCR and one ELISA techniques for the detection of small ruminant lentiviruses (SRLVs) in milk of sheep and goats.
[So] Source:Res Vet Sci;94(3):817-9, 2013 Jun.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of the present study was to compare the efficiency of two PCR techniques for the diagnosis of small ruminant lentiviruses (SRLVs). Detection of the proviral genome by PCR, though sensitive, is difficult due to the heterogeneity of the SRLV genomes. One of the PCR techniques amplifies a fragment in the pol gene (pol-PCR) and the other PCR targets the LTR region of the proviral genome (LTR-PCR). Milk from 194 sheep and 163 goats from farms in the Central Spain was analyzed by both techniques and compared to results obtained by ELISA. When compared to the serologic assay, the agreement of both PCR techniques was very low (0.024 and 0.020 in sheep, and 0.124 and 0.114 in goats). In view of these results, it may be concluded that the efficacy of PCR for the diagnosis of SRLVs is low and a combination of PCR and ELISA should be used for diagnosis.
[Mh] Termos MeSH primário: Ensaio de Imunoadsorção Enzimática/veterinária
Cabras/virologia
Lentivirus Ovinos-Caprinos/metabolismo
Leite/virologia
Reação em Cadeia da Polimerase/veterinária
Ovinos/virologia
[Mh] Termos MeSH secundário: Animais
Ensaio de Imunoadsorção Enzimática/métodos
Feminino
Doenças das Cabras/virologia
Infecções por Lentivirus/veterinária
Infecções por Lentivirus/virologia
Lentivirus Ovinos-Caprinos/genética
Lentivirus Ovinos-Caprinos/imunologia
Reação em Cadeia da Polimerase/métodos
Sensibilidade e Especificidade
Doenças dos Ovinos/virologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1312
[Cu] Atualização por classe:130409
[Lr] Data última revisão:
130409
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130207
[St] Status:MEDLINE



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