Base de dados : MEDLINE
Pesquisa : B05.275 [Categoria DeCS]
Referências encontradas : 542 [refinar]
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  1 / 542 MEDLINE  
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[PMID]:28465022
[Au] Autor:Wu D; Shi J; Peng K; Sheng J; Wang J; Wang B; Hong Y
[Ad] Endereço:School of Life Sciences, Nanchang University, Nanchang, China.
[Ti] Título:Structural characteristics of gonadal development and hermaphroditic phenomenon in freshwater pearl mussel, Sinohyriopisis schlegelii (Hyriopisis schlegelii).
[So] Source:Tissue Cell;49(3):440-446, 2017 Jun.
[Is] ISSN:1532-3072
[Cp] País de publicação:Scotland
[La] Idioma:eng
[Ab] Resumo:The period of gonads development was first studied from one to five years in the freshwater pearl mussel Hyriopisis schlegelii. It lasted for 36 months and was divided into three main stages: initiation of gonad formation, a stable growth phase, and a reproductive cell development phase. Each reproductive cycle consisted of five stages: proliferative stage (from late January to late February), growth stage (from late February to late March), maturation stage, spawning stage (from early April to late October) and recovery stage (from early November to late January). Interestingly, a hermaphroditic phenomenon was observed in this mussel for the first time, which appears during the development stage from 26 to 32 months. Male and female follicular tissues coexisted in hermaphrodite individuals with the male follicular tissue accounting for more than 90% of the whole gonad tissue. No hermaphroditic phenomenon was observed in matured gonad. We thus speculate that self-fertilization does not exist in H. schlegelii.
[Mh] Termos MeSH primário: Gônadas
Organismos Hermafroditas
Unionidae
[Mh] Termos MeSH secundário: Animais
Gônadas/citologia
Gônadas/fisiologia
Organismos Hermafroditas/citologia
Organismos Hermafroditas/fisiologia
Reprodução/fisiologia
Unionidae/citologia
Unionidae/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


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[PMID]:29073214
[Au] Autor:Wu GC; Luo JW; Li HW; Huang CH; Chang CF
[Ad] Endereço:Department of Aquaculture, National Taiwan Ocean University, Keelung, Taiwan.
[Ti] Título:Robust gdf9 and bmp15 expression in the oocytes of ovotestes through the Figla-independent pathway in the hermaphroditic black porgy, Acanthopagrus schlegelii.
[So] Source:PLoS One;12(10):e0186991, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:More than 1,500 fish species are hermaphroditic, but no hermaphroditic lineage appears to be evolutionarily ancient in fishes. Thus, whether more than one sex at a time was present during the evolutionary shift from gonochorism to hermaphroditism in fishes is an intriguing question. Ectopic oocytes were created in the ovotestes of protandrous black porgy via the withdrawal of estradiol (E2) administration. These ectopic oocytes reprogrammed the surrounding cells, which changed from Sertoli cells to follicle-like cells. We observed that gdf9 and bmp15 expression was localized in the primary oocytes and gradually decreased after oocytes entered a secondary oocyte stage. Robust expression of gdf9 and bmp15 in ectopic oocytes was associated with the surrounding Sertoli cells. However, blocking Cyp19a1a activity and increasing androgen levels did not stimulate the expression of gdf9 and bmp15. Thus, the robust gdf9 and bmp15 expression was not related to the inappropriate male microenvironment. Furthermore, in vitro data demonstrated that gdf9 and bmp15 were not downstream genes of Figla signaling. Therefore, our results suggest that there are two independent mechanisms, a Figla-dependent pathway and a Figla-independent pathway, by which oocyte-surrounding cells are altered from a male somatic fate to a female somatic fate. This functional switch might clarify how oocytes created an appropriate microenvironment during the transition from the ancient gonochorism to the present hermaphroditism.
[Mh] Termos MeSH primário: Proteína Morfogenética Óssea 15/genética
Regulação da Expressão Gênica
Fator 9 de Diferenciação de Crescimento/genética
Oócitos/metabolismo
Ovário/citologia
Perciformes/genética
Testículo/citologia
[Mh] Termos MeSH secundário: Animais
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo
Estradiol/farmacologia
Feminino
Proteínas de Peixes/genética
Regulação da Expressão Gênica/efeitos dos fármacos
Organismos Hermafroditas/citologia
Organismos Hermafroditas/efeitos dos fármacos
Organismos Hermafroditas/genética
Organismos Hermafroditas/fisiologia
Masculino
Perciformes/metabolismo
Perciformes/fisiologia
Células de Sertoli/metabolismo
Transdução de Sinais/efeitos dos fármacos
Vitelogênese/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Basic Helix-Loop-Helix Transcription Factors); 0 (Bone Morphogenetic Protein 15); 0 (Fish Proteins); 0 (Growth Differentiation Factor 9); 4TI98Z838E (Estradiol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171027
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186991


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[PMID]:28844904
[Au] Autor:McCaig CM; Lin X; Farrell M; Rehain-Bell K; Shakes DC
[Ad] Endereço:Department of Biology, College of William and Mary, Williamsburg, VA 23187, USA.
[Ti] Título:Germ cell cysts and simultaneous sperm and oocyte production in a hermaphroditic nematode.
[So] Source:Dev Biol;430(2):362-373, 2017 10 15.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Studies of gamete development in the self-fertile hermaphrodites of Caenorhabditis elegans have significantly contributed to our understanding of fundamental developmental mechanisms. However, evolutionary transitions from outcrossing males and females to self-fertile hermaphrodites have convergently evolved within multiple nematode sub-lineages, and whether the C. elegans pattern of self-fertile hermaphroditism and gamete development is representative remains largely unexplored. Here we describe a pattern of sperm production in the trioecious (male/female/hermaphrodite) nematode Rhabditis sp. SB347 (recently named Auanema rhodensis) that differs from C. elegans in two striking ways. First, while C. elegans hermaphrodites make a one-time switch from sperm to oocyte production, R. sp. SB347 hermaphrodites continuously produce both sperm and oocytes. Secondly, while C. elegans germ cell proliferation is limited to germline stem cells (GSCs), sperm production in R. sp. SB347 includes an additional population of mitotically dividing cells that are a developmental intermediate between GSCs and fully differentiated spermatocytes. These cells are present in males and hermaphrodites but not females, and exhibit key characteristics of spermatogonia - the mitotic progenitors of spermatocytes in flies and vertebrates. Specifically, they exist outside the stem cell niche, increase germ cell numbers by transit-amplifying divisions, and synchronously proliferate within germ cell cysts. We also discovered spermatogonia in other trioecious Rhabditis species, but not in the male/female species Rhabditis axei or the more distant hermaphroditic Oscheius tipulae. The discovery of simultaneous hermaphroditism and spermatogonia in a lab-cultivatable nematode suggests R. sp. SB347 as a richly informative species for comparative studies of gametogenesis.
[Mh] Termos MeSH primário: Organismos Hermafroditas/fisiologia
Oogênese/fisiologia
Óvulo/citologia
Rabditídios/fisiologia
Espermatogênese/fisiologia
Espermatozoides/citologia
[Mh] Termos MeSH secundário: Animais
Caenorhabditis elegans/crescimento & desenvolvimento
Caenorhabditis elegans/fisiologia
Feminino
Masculino
Rabditídios/crescimento & desenvolvimento
Processos de Determinação Sexual
Especificidade da Espécie
Espermatogônias/fisiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE


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[PMID]:28827395
[Au] Autor:Winter ES; Schwarz A; Fabig G; Feldman JL; Pires-daSilva A; Müller-Reichert T; Sadler PL; Shakes DC
[Ad] Endereço:Department of Biology, College of William and Mary, Williamsburg, VA 23187, USA.
[Ti] Título:Cytoskeletal variations in an asymmetric cell division support diversity in nematode sperm size and sex ratios.
[So] Source:Development;144(18):3253-3263, 2017 09 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Asymmetric partitioning is an essential component of many developmental processes. As spermatogenesis concludes, sperm are streamlined by discarding unnecessary cellular components into cellular wastebags called residual bodies (RBs). During nematode spermatogenesis, this asymmetric partitioning event occurs shortly after anaphase II, and both microtubules and actin partition into a central RB. Here, we use fluorescence and transmission electron microscopy to elucidate and compare the intermediate steps of RB formation in , sp. SB347 (recently named ) and related nematodes. In all cases, intact microtubules reorganize and move from centrosomal to non-centrosomal sites at the RB-sperm boundary whereas actin reorganizes through cortical ring expansion and clearance from the poles. However, in species with tiny spermatocytes, these cytoskeletal changes are restricted to one pole. Consequently, partitioning yields one functional sperm with the X-bearing chromosome complement and an RB with the other chromosome set. Unipolar partitioning may not require an unpaired X, as it also occurs in XX spermatocytes. Instead, constraints related to spermatocyte downsizing may have contributed to the evolution of a sperm cell equivalent to female polar bodies.
[Mh] Termos MeSH primário: Divisão Celular Assimétrica
Caenorhabditis elegans/citologia
Tamanho Celular
Citoesqueleto/metabolismo
Razão de Masculinidade
Espermatozoides/citologia
[Mh] Termos MeSH secundário: Actinas/metabolismo
Animais
Centrossomo/metabolismo
Citoesqueleto/ultraestrutura
Feminino
Organismos Hermafroditas/citologia
Masculino
Meiose
Microtúbulos/metabolismo
Modelos Biológicos
Espermatócitos/citologia
Espermatócitos/ultraestrutura
Espermatogênese
Espermatozoides/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Actins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1242/dev.153841


  5 / 542 MEDLINE  
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[PMID]:28712675
[Au] Autor:Lodi M; Meijer FW; Koene JM
[Ad] Endereço:Section of Animal Ecology, Department of Ecological Science, VU University Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands; Naturalis Biodiversity Center, Darwinweg 2, 2333 CR Leiden, The Netherlands. Electronic address: m.lodi@vu.nl.
[Ti] Título:Ejaculates are not used as nuptial gifts in simultaneously hermaphroditic snails.
[So] Source:Zoology (Jena);123:30-36, 2017 Aug.
[Is] ISSN:1873-2720
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Promoted by sexual selection, males usually adopt different ways to increase their fertilization chances. In many insect taxa males donate nuptial gifts, together with sperm, which represent a valuable additional nutrient source that females can use to provision eggs. This has also been suggested to occur in simultaneous hermaphrodites, organisms with both sex functions. In theory, donation of nuptial gifts or extra nutrients might work in hermaphrodites that mate unilaterally (one-way donation of ejaculates), but will not be effective when these organisms mate reciprocally (mutual exchange of ejaculates), since on average each partner would receive the amount it also transfers. Hence, for the latter the net amount gained would be zero, and when considering the non-trivial costs of metabolic conversion the energy balance of this exchange ends up negative. To test this prediction, we measured the material (dry weight) and resource (carbon and nitrogen content) investment into ejaculates of the unilaterally mating freshwater snail Lymnaea stagnalis and spermatophores of the reciprocally mating land snail Cornu aspersum. When compared to eggs, our measurements indicate that the investment is low for ejaculates and spermatophores, neither of which represent a significant contribution to egg production. Importantly, during reciprocal matings, couples exchanged similar amounts of material and resources, thus a gain of extra substances seems irrelevant. Hence, caution is needed when generalizing functions of male reproductive strategies across mating systems. Although digestion of ejaculates does not provide extra material and resources in simultaneous hermaphrodites, their absorption could still be important to eliminate an excess of received sperm and to select sperm via cryptic female choice.
[Mh] Termos MeSH primário: Organismos Hermafroditas/fisiologia
Comportamento Sexual Animal/fisiologia
[Mh] Termos MeSH secundário: Animais
Ejaculação
Feminino
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE


  6 / 542 MEDLINE  
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[PMID]:28662030
[Au] Autor:Hoang HD; Miller MA
[Ad] Endereço:Department of Cell, Developmental and Integrative Biology, University of Alabama School of Medicine, Birmingham, Alabama, United States of America.
[Ti] Título:Chemosensory and hyperoxia circuits in C. elegans males influence sperm navigational capacity.
[So] Source:PLoS Biol;15(6):e2002047, 2017 Jun.
[Is] ISSN:1545-7885
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The sperm's crucial function is to locate and fuse with a mature oocyte. Under laboratory conditions, Caenorhabditis elegans sperm are very efficient at navigating the hermaphrodite reproductive tract and locating oocytes. Here, we identify chemosensory and oxygen-sensing circuits that affect the sperm's navigational capacity. Multiple Serpentine Receptor B (SRB) chemosensory receptors regulate Gα pathways in gustatory sensory neurons that extend cilia through the male nose. SRB signaling is necessary and sufficient in these sensory neurons to influence sperm motility parameters. The neuropeptide Y pathway acts together with SRB-13 to antagonize negative effects of the GCY-35 hyperoxia sensor on spermatogenesis. SRB chemoreceptors are not essential for sperm navigation under low oxygen conditions that C. elegans prefers. In ambient oxygen environments, SRB-13 signaling impacts gene expression during spermatogenesis and the sperm's mitochondria, thereby increasing migration velocity and inhibiting reversals within the hermaphrodite uterus. The SRB-13 transcriptome is highly enriched in genes implicated in pathogen defense, many of which are expressed in diverse tissues. We show that the critical time period for SRB-13 signaling is prior to spermatocyte differentiation. Our results support the model that young C. elegans males sense external environment and oxygen tension, triggering long-lasting downstream signaling events with effects on the sperm's mitochondria and navigational capacity. Environmental exposures early in male life may alter sperm function and fertility.
[Mh] Termos MeSH primário: Caenorhabditis elegans/fisiologia
Células Quimiorreceptoras/fisiologia
Células Receptoras Sensoriais/fisiologia
Motilidade Espermática/fisiologia
Espermatozoides/fisiologia
[Mh] Termos MeSH secundário: Aerobiose
Animais
Animais Geneticamente Modificados
Caenorhabditis elegans/genética
Caenorhabditis elegans/metabolismo
Proteínas de Caenorhabditis elegans/genética
Proteínas de Caenorhabditis elegans/metabolismo
Células Quimiorreceptoras/metabolismo
Feminino
Organismos Hermafroditas/genética
Organismos Hermafroditas/fisiologia
Masculino
Microscopia de Fluorescência
Mutação
Oócitos/fisiologia
Oxigênio/metabolismo
Receptores Acoplados a Proteínas-G/genética
Receptores Acoplados a Proteínas-G/metabolismo
Células Receptoras Sensoriais/metabolismo
Motilidade Espermática/genética
Espermatozoides/metabolismo
Imagem com Lapso de Tempo/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (Receptors, G-Protein-Coupled); S88TT14065 (Oxygen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pbio.2002047


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[PMID]:28648844
[Au] Autor:Boateng R; Nguyen KCQ; Hall DH; Golden A; Allen AK
[Ad] Endereço:Department of Biology, Howard University, Washington, DC 20059, USA.
[Ti] Título:Novel functions for the RNA-binding protein ETR-1 in Caenorhabditis elegans reproduction and engulfment of germline apoptotic cell corpses.
[So] Source:Dev Biol;429(1):306-320, 2017 09 01.
[Is] ISSN:1095-564X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RNA-binding proteins (RBPs) are essential regulators of gene expression that act through a variety of mechanisms to ensure the proper post-transcriptional regulation of their target RNAs. RBPs in multiple species have been identified as playing crucial roles during development and as having important functions in various adult organ systems, including the heart, nervous, muscle, and reproductive systems. ETR-1, a highly conserved ELAV-Type RNA-binding protein belonging to the CELF/Bruno protein family, has been previously reported to be involved in C. elegans muscle development. Animals depleted of ETR-1 have been previously characterized as arresting at the two-fold stage of embryogenesis. In this study, we show that ETR-1 is expressed in the hermaphrodite somatic gonad and germ line, and that reduction of ETR-1 via RNA interference (RNAi) results in reduced hermaphrodite fecundity. Detailed characterization of this fertility defect indicates that ETR-1 is required in both the somatic tissue and the germ line to ensure wild-type reproductive levels. Additionally, the ability of ETR-1 depletion to suppress the published WEE-1.3-depletion infertility phenotype is dependent on ETR-1 being reduced in the soma. Within the germline of etr-1(RNAi) hermaphrodite animals, we observe a decrease in average oocyte size and an increase in the number of germline apoptotic cell corpses as evident by an increased number of CED-1::GFP and acridine orange positive apoptotic germ cells. Transmission Electron Microscopy (TEM) studies confirm the significant increase in apoptotic cells in ETR-1-depleted animals, and reveal a failure of the somatic gonadal sheath cells to properly engulf dying germ cells in etr-1(RNAi) animals. Through investigation of an established engulfment pathway in C. elegans, we demonstrate that co-depletion of CED-1 and ETR-1 suppresses both the reduced fecundity and the increase in the number of apoptotic cell corpses observed in etr-1(RNAi) animals. Combined, this data identifies a novel role for ETR-1 in hermaphrodite gametogenesis and in the process of engulfment of germline apoptotic cell corpses.
[Mh] Termos MeSH primário: Apoptose
Proteínas de Caenorhabditis elegans/metabolismo
Caenorhabditis elegans/citologia
Caenorhabditis elegans/metabolismo
Células Germinativas/metabolismo
Fagocitose
Proteínas de Ligação a RNA/metabolismo
[Mh] Termos MeSH secundário: Animais
Caenorhabditis elegans/ultraestrutura
Tamanho Celular
Embrião não Mamífero/citologia
Embrião não Mamífero/metabolismo
Fertilidade
Deleção de Genes
Células Germinativas/citologia
Células Germinativas/ultraestrutura
Gônadas/metabolismo
Organismos Hermafroditas/metabolismo
Mitose
Oócitos/citologia
Ovulação
Fenótipo
Interferência de RNA
Reprodução
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, N.I.H., INTRAMURAL; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Caenorhabditis elegans Proteins); 0 (ETR-1 protein, C elegans); 0 (RNA-Binding Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE


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[PMID]:28645154
[Au] Autor:Brown KC; Svendsen JM; Tucci RM; Montgomery BE; Montgomery TA
[Ad] Endereço:Department of Biology, Colorado State University, Fort Collins, CO 80523, USA.
[Ti] Título:ALG-5 is a miRNA-associated Argonaute required for proper developmental timing in the Caenorhabditis elegans germline.
[So] Source:Nucleic Acids Res;45(15):9093-9107, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Caenorhabditis elegans contains 25 Argonautes, of which, ALG-1 and ALG-2 are known to primarily interact with miRNAs. ALG-5 belongs to the AGO subfamily of Argonautes that includes ALG-1 and ALG-2, but its role in small RNA pathways is unknown. We analyzed by high-throughput sequencing the small RNAs associated with ALG-5, ALG-1 and ALG-2, as well as changes in mRNA expression in alg-5, alg-1 and alg-2 mutants. We show that ALG-5 defines a distinct branch of the miRNA pathway affecting the expression of genes involved in immunity, defense, and development. In contrast to ALG-1 and ALG-2, which associate with most miRNAs and have general roles throughout development, ALG-5 interacts with only a small subset of miRNAs and is specifically expressed in the germline where it localizes alongside the piRNA and siRNA machinery at P granules. alg-5 is required for optimal fertility and mutations in alg-5 lead to a precocious transition from spermatogenesis to oogenesis. Our results provide a near-comprehensive analysis of miRNA-Argonaute interactions in C. elegans and reveal a new role for miRNAs in the germline.
[Mh] Termos MeSH primário: Proteínas Argonauta/genética
Proteínas de Caenorhabditis elegans/genética
Caenorhabditis elegans/genética
Regulação da Expressão Gênica no Desenvolvimento
Células Germinativas/metabolismo
RNA de Helmintos/genética
Proteínas de Ligação a RNA/genética
[Mh] Termos MeSH secundário: Animais
Proteínas Argonauta/metabolismo
Caenorhabditis elegans/classificação
Caenorhabditis elegans/crescimento & desenvolvimento
Caenorhabditis elegans/metabolismo
Proteínas de Caenorhabditis elegans/metabolismo
Células Germinativas/crescimento & desenvolvimento
Organismos Hermafroditas/genética
Organismos Hermafroditas/crescimento & desenvolvimento
Organismos Hermafroditas/metabolismo
Sequenciamento de Nucleotídeos em Larga Escala
MicroRNAs/genética
MicroRNAs/metabolismo
Mutação
Oogênese/genética
Filogenia
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
RNA de Helmintos/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Interferente Pequeno/genética
RNA Interferente Pequeno/metabolismo
Proteínas de Ligação a RNA/metabolismo
Espermatogênese/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (ALG-1 protein, C elegans); 0 (ALG-2 protein, C elegans); 0 (ALG-5 protein, C elegans); 0 (Argonaute Proteins); 0 (Caenorhabditis elegans Proteins); 0 (MicroRNAs); 0 (Protein Isoforms); 0 (RNA, Helminth); 0 (RNA, Messenger); 0 (RNA, Small Interfering); 0 (RNA-Binding Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170625
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx536


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[PMID]:28474376
[Au] Autor:Shihab I; Gopalakrishnan A; Vineesh N; Muktha M; Akhilesh KV; Vijayagopal P
[Ad] Endereço:Marine Biotechnology Division, ICAR-Central Marine Fisheries Research Institute, P. B. No. 1603, Ernakulum North, P. O., Kochi, 682 018, Kerala, India.
[Ti] Título:Histological profiling of gonads depicting protandrous hermaphroditism in Eleutheronema tetradactylum.
[So] Source:J Fish Biol;90(6):2402-2411, 2017 Jun.
[Is] ISSN:1095-8649
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The fourfinger threadfin Eleutheronema tetradactylum is reported as a protandrous hermaphrodite from Australian waters, while being a gonochorist in reports from Singapore and India, with a single report of protandrous hermaphroditism from the latter. Histological analysis of gonads of fish from Indian waters confirms protandrous hermaphroditism in E. tetradactylum. The study was based on 480 fish examined from eight locations along the Indian coast. Mean total length (L ) of male fish was 240 mm with the transition to female starting from 280 mm L . Specimens confirmed as mature females were >380 mm L .
[Mh] Termos MeSH primário: Peixes/anatomia & histologia
Organismos Hermafroditas/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Animais
Feminino
Gônadas
Índia
Masculino
Ovário/anatomia & histologia
Ovário/crescimento & desenvolvimento
Caracteres Sexuais
Maturidade Sexual
Testículo/anatomia & histologia
Testículo/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE
[do] DOI:10.1111/jfb.13324


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[PMID]:28400332
[Au] Autor:Sun ZH; Zhou L; Li Z; Liu XC; Li SS; Wang Y; Gui JF
[Ad] Endereço:State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of the Chinese Academy of Sciences, Wuhan 430072, China.
[Ti] Título:Sexual dimorphic expression of dnd in germ cells during sex reversal and its requirement for primordial germ cell survival in protogynous hermaphroditic grouper.
[So] Source:Comp Biochem Physiol B Biochem Mol Biol;208-209:47-57, 2017 Jun.
[Is] ISSN:1879-1107
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Dead end (dnd), vertebrate-specific germ cell marker, had been demonstrated to be essential for primordial germ cell (PGC) migration and survival, and the link between PGC number and sex change had been revealed in some teleost species, but little is known about dnd in hermaphroditic vertebrates. In the present study, a protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides) dnd homologue (Ecdnd) was identified and characterized. Quantitative real-time PCR and in situ hybridization analysis revealed a dynamic and sexually dimorphic expression pattern in PGCs and germ cells of gonads. During sex changing, the Ecdnd transcript sharply increased in early transitional gonad, reached the highest level at late transitional gonad stage, and decreased after testis maturation. Visualization of zebrafish PGCs by injecting with RFP-Ecdnd-3'UTR RNA and GFP-zfnanos3-3'UTR RNA confirmed importance of Ecdnd 3'UTR for the PGC distribution. In addition, knockdown of EcDnd by using antisense morpholinos (MO) caused the ablation of PGCs in orange-spotted grouper. Therefore, the current data indicate that Ecdnd is essential for PGCs survival and may serve as a useful germ cell marker during gametogenesis in hermaphroditic grouper.
[Mh] Termos MeSH primário: Proteínas de Peixes/metabolismo
Células Germinativas/citologia
Gônadas/metabolismo
Organismos Hermafroditas/metabolismo
Caracteres Sexuais
Diferenciação Sexual
Peixe-Zebra/metabolismo
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Feminino
Proteínas de Peixes/genética
Regulação da Expressão Gênica
Regulação da Expressão Gênica no Desenvolvimento
Células Germinativas/metabolismo
Gônadas/embriologia
Organismos Hermafroditas/genética
Organismos Hermafroditas/crescimento & desenvolvimento
Hibridização In Situ
Masculino
Filogenia
Homologia de Sequência de Aminoácidos
Peixe-Zebra/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fish Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE



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