[PMID]: | 28981863 |
[Au] Autor: | Tashiro S; Nishihara Y; Kugou K; Ohta K; Kanoh J |
[Ad] Endereço: | Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan. |
[Ti] Título: | Subtelomeres constitute a safeguard for gene expression and chromosome homeostasis. |
[So] Source: | Nucleic Acids Res;45(18):10333-10349, 2017 Oct 13. |
[Is] ISSN: | 1362-4962 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | The subtelomere, a telomere-adjacent chromosomal domain, contains species-specific homologous DNA sequences, in addition to various genes. However, the functions of subtelomeres, particularly subtelomeric homologous (SH) sequences, remain elusive. Here, we report the first comprehensive analyses of the cellular functions of SH sequences in the fission yeast, Schizosaccharomyces pombe. Complete removal of SH sequences from the genome revealed that they are dispensable for mitosis, meiosis and telomere length control. However, when telomeres are lost, SH sequences prevent deleterious inter-chromosomal end fusion by facilitating intra-chromosomal circularization. Surprisingly, SH-deleted cells sometimes survive telomere loss through inter-chromosomal end fusions via homologous loci such as LTRs, accompanied by centromere inactivation of either chromosome. Moreover, SH sequences function as a buffer region against the spreading of subtelomeric heterochromatin into the neighboring gene-rich regions. Furthermore, we found a nucleosome-free region at the subtelomeric border, which may be a second barrier that blocks heterochromatin spreading into the subtelomere-adjacent euchromatin. Thus, our results demonstrate multiple defense functions of subtelomeres in chromosome homeostasis and gene expression. |
[Mh] Termos MeSH primário: |
Cromossomos Fúngicos/fisiologia Expressão Gênica Homeostase/genética Proteínas de Schizosaccharomyces pombe/genética Schizosaccharomyces/genética Telômero/fisiologia
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[Mh] Termos MeSH secundário: |
Centrômero/metabolismo Instabilidade Cromossômica/genética Regulação Fúngica da Expressão Gênica Heterocromatina/metabolismo Organismos Geneticamente Modificados Deleção de Sequência Proteínas de Ligação a Telômeros/metabolismo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Heterochromatin); 0 (Schizosaccharomyces pombe Proteins); 0 (Telomere-Binding Proteins) |
[Em] Mês de entrada: | 1710 |
[Cu] Atualização por classe: | 171031 |
[Lr] Data última revisão:
| 171031 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 171006 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1093/nar/gkx780 |
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