Base de dados : MEDLINE
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  1 / 13 MEDLINE  
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[PMID]:29256425
[Au] Autor:de Buron I; Hill-Spanik KM; Haselden L; Atkinson SD; Hallett SL; Arnott SA
[Ad] Endereço:Department of Biology, College of Charleston, Charleston, South Carolina 29412, USA.
[Ti] Título:Infection dynamics of Kudoa inornata (Cnidaria: Myxosporea) in spotted seatrout Cynoscion nebulosus (Teleostei: Sciaenidae).
[So] Source:Dis Aquat Organ;127(1):29-40, 2017 Dec 19.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Kudoa inornata is a myxosporean parasite that develops in the somatic muscle of spotted seatrout Cynoscion nebulosus, an economically and ecologically important fish in estuaries and harbors in southeastern North America. In South Carolina (SC), USA, over 90% of wild adult spotted seatrout are infected. To inform potential mitigation strategies, we conducted 3 experiments using naïve sentinel seatrout and infectious stages of K. inornata naturally present in raw water from Charleston Harbor, SC, to determine (1) if K. inornata infection follows a seasonal pattern, and (2) how long it takes for myxospores to develop in fish muscle. Infection by K. inornata was determined by visual detection of myxospores in fish muscle squashes, and any visually negative samples were then assayed for K. inornata ribosomal DNA using novel parasite-specific PCR primers. We observed that K. inornata infection in seatrout followed a seasonal pattern, with high prevalence when water temperature was highest (27-31°C; July-September) and infections that were either covert (at ~13-15°C) or not detected (<13°C) at the lowest water temperatures in January-February. Myxospore development occurred within 476 degree-days, i.e. 2 wk in a typical SC summer. Infection was dependent on fish density, which limited presumptive actinospore dose. Our findings suggest that the life cycle of the parasite may be disrupted by preventing spore-rich seatrout carcasses (e.g. at angler cleaning stations) being thrown back into harbors and estuaries throughout the year.
[Mh] Termos MeSH primário: Cnidários/fisiologia
Doenças dos Peixes/parasitologia
Peixes/parasitologia
Doenças Parasitárias em Animais/parasitologia
[Mh] Termos MeSH secundário: Animais
Aquicultura
Seres Humanos
Espécies Sentinelas
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE
[do] DOI:10.3354/dao03174


  2 / 13 MEDLINE  
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[PMID]:28462753
[Au] Autor:Tulloch JSP; McGinley L; Sánchez-Vizcaíno F; Medlock JM; Radford AD
[Ad] Endereço:NIHR Health Protection Research Unit in Emerging and Zoonotic Infections, University of Liverpool,Liverpool,UK.
[Ti] Título:The passive surveillance of ticks using companion animal electronic health records.
[So] Source:Epidemiol Infect;145(10):2020-2029, 2017 07.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ticks represent a large global reservoir of zoonotic disease. Current surveillance systems can be time and labour intensive. We propose that the passive surveillance of companion animal electronic health records (EHRs) could provide a novel methodology for describing temporal and spatial tick activity. A total of 16 58 857 EHRs were collected over a 2-year period (31 March 2014 and 29 May 2016) from companion animals attending a large sentinel network of 192 veterinary clinics across Great Britain (the Small Animal Veterinary Surveillance Network - SAVSNET). In total, 2180 EHRs were identified where a tick was recorded on an animal. The relative risk of dogs presenting with a tick compared with cats was 0·73 (95% confidence intervals 0·67-0·80). The highest number of tick records were in the south central regions of England. The presence of ticks showed marked seasonality with summer peaks, and a secondary smaller peak in autumn for cats; ticks were still being found throughout most of Great Britain during the winter. This suggests that passive surveillance of companion animal EHRs can describe tick activity temporally and spatially in a large cohort of veterinary clinics across Great Britain. These results and methodology could help inform veterinary and public health messages as well as increase awareness of ticks and tick-borne diseases in the general population.
[Mh] Termos MeSH primário: Doenças do Gato/epidemiologia
Doenças do Cão/epidemiologia
Registros Eletrônicos de Saúde/utilização
Monitoramento Epidemiológico/veterinária
Vigilância de Evento Sentinela/veterinária
Carrapatos/fisiologia
[Mh] Termos MeSH secundário: Animais
Doenças do Gato/parasitologia
Gatos
Doenças do Cão/parasitologia
Cães
Animais de Estimação
Espécies Sentinelas/parasitologia
Doenças Transmitidas por Carrapatos/epidemiologia
Doenças Transmitidas por Carrapatos/parasitologia
Reino Unido/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1017/S0950268817000826


  3 / 13 MEDLINE  
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[PMID]:28285585
[Au] Autor:Irwin PJ; Robertson ID; Westman ME; Perkins M; Straubinger RK
[Ad] Endereço:Vector and Water-Borne Pathogen Research Group, School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia. P.Irwin@murdoch.edu.au.
[Ti] Título:Searching for Lyme borreliosis in Australia: results of a canine sentinel study.
[So] Source:Parasit Vectors;10(1):114, 2017 Mar 13.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Lyme borreliosis is a common tick-borne disease of the northern hemisphere that is caused by bacterial spirochaetes of the Borrelia burgdorferi (sensu lato) (Bbsl) complex. To date, there has been no convincing evidence for locally-acquired Lyme borreliosis on the Australian continent and there is currently a national debate concerning the nature and distributions of zoonotic tick-transmitted infectious disease in Australia. In studies conducted in Europe and the United States, dogs have been used as sentinels for tick-associated illness in people since they readily contact ticks that may harbour zoonotic pathogens. Applying this principle, we used a combination of serological assays to test dogs living in tick 'hot spots' and exposed to the Australian paralysis tick, Ixodes holocyclus, for evidence of exposure to B. burgdorferi (s.l.) antigens and other vector-borne pathogens. RESULTS: Altogether, 555 dogs from four demographic groups were recruited into this study. One dog had evidence of exposure to Anaplasma spp. but no other dog was positive in screening tests. A total of 122 dogs (22.0%) had a kinetic ELISA (KELA) unit value > 100, and one dog with a high titre (399.9 KELA units) had been vaccinated against B. burgdorferi (sensu stricto) before travelling to Australia. Older dogs and those with a history of tick paralysis were significantly more likely to have a KELA unit value > 100. Line immunoassay analysis revealed moderate-to-weak (equivocal) bands in 27 (4.9%) dogs. CONCLUSIONS: Except for a single dog presumed to have been exposed to Anaplasma platys, infection with Anaplasma spp. B. burgdorferi (s.l.), Ehrlichia spp., and Dirofilaria immitis, was not detected in the cohort of Australian dogs evaluated in this study. These results provide further evidence that Lyme borreliosis does not exist in Australia but that cross-reacting antibodies (false positive results) are common and may be caused by the transmission of other tick-associated organisms.
[Mh] Termos MeSH primário: Doenças do Cão/epidemiologia
Doença de Lyme/veterinária
Espécies Sentinelas/microbiologia
Espécies Sentinelas/parasitologia
Doenças Transmitidas por Carrapatos/veterinária
[Mh] Termos MeSH secundário: Anaplasma/imunologia
Animais
Anticorpos Antibacterianos/sangue
Austrália/epidemiologia
Borrelia burgdorferi/imunologia
Borrelia burgdorferi/isolamento & purificação
Doenças do Cão/diagnóstico
Doenças do Cão/imunologia
Doenças do Cão/microbiologia
Cães
Ehrlichia/imunologia
Ehrlichiose/veterinária
Ensaio de Imunoadsorção Enzimática
Ixodes/microbiologia
Doença de Lyme/diagnóstico
Doença de Lyme/epidemiologia
Doença de Lyme/imunologia
Espécies Sentinelas/imunologia
Testes Sorológicos
Doenças Transmitidas por Carrapatos/epidemiologia
Doenças Transmitidas por Carrapatos/imunologia
Doenças Transmitidas por Carrapatos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170314
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2058-z


  4 / 13 MEDLINE  
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[PMID]:28268185
[Au] Autor:Xu J; Duan ZL; Guan ZX; Wang YY; Lin C; Zhang TT; Zhang HQ; Qian X; Xia CM
[Ad] Endereço:Department of Parasitology, Medical College of Soochow University, Suzhou, 215123, Jiangsu Province, China. Electronic address: jxu2014@suda.edu.cn.
[Ti] Título:Early detection of circulating DNA of Schistosoma japonicum in sentinel mice models.
[So] Source:Exp Parasitol;176:82-88, 2017 May.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Currently in China, the schistosomiasis control program has shifted its focus from transmission control to the elimination of the disease. Effective forecast and surveillance systems of schistiosomiasis are of great importance for issuing timely and early warnings on risk of infection, and therefore implementing preventive measures to avoid infection. There is great demand in more sensitive and specific methods to improve the surveillance system for early detection of S. japonicum infection in sentinel mice. In this study, we reported a sensitive nested-PCR assay targeting a 303-bp fragment from highly repetitive retrotransposon SjCHGCS19 to detect the S. japonicum DNA in sera of experimental mice. Meanwhile, detection efficacy of the nested-PCR was compared with two conventional methods for field monitoring schistosomiasis such as ELISA and IHA. The nested-PCR assay could detect the specific DNA at 3-day post-infection in sera of mice with 5 cercariae infection, while for ELISA and IHA, both show negative results even after 2 weeks post-infection in mice with 20 cercariae infection. Our results demonstrated the DNA-based assay was more sensitive to make early diagnosis of S. japonicum infection in sentinel mice models, which will improve the early-warning ability of schistosomiasis surveillance system.
[Mh] Termos MeSH primário: DNA de Helmintos/sangue
Schistosoma japonicum/genética
Esquistossomose Japônica/diagnóstico
[Mh] Termos MeSH secundário: Animais
Anticorpos Anti-Helmínticos/sangue
Modelos Animais de Doenças
Ensaio de Imunoadsorção Enzimática
Feminino
Testes de Hemaglutinação
Masculino
Camundongos
Camundongos Endogâmicos ICR
Reação em Cadeia da Polimerase
Distribuição Aleatória
Schistosoma japonicum/imunologia
Schistosoma japonicum/isolamento & purificação
Esquistossomose Japônica/sangue
Esquistossomose Japônica/parasitologia
Sensibilidade e Especificidade
Espécies Sentinelas
Caramujos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (DNA, Helminth)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE


  5 / 13 MEDLINE  
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[PMID]:27839718
[Au] Autor:Burgeot T; Akcha F; Ménard D; Robinson C; Loizeau V; Brach-Papa C; Martínez-Gòmez C; Le Goff J; Budzinski H; Le Menach K; Cachot J; Minier C; Broeg K; Hylland K
[Ad] Endereço:Department of Biogeochemistry and Ecotoxicology, Ifremer, Rue de l'Ile d'Yeu, BP 21105, 44311, Nantes cedex 03, France. Electronic address: tburgeot@ifremer.fr.
[Ti] Título:Integrated monitoring of chemicals and their effects on four sentinel species, Limanda limanda, Platichthys flesus, Nucella lapillus and Mytilus sp., in Seine Bay: A key step towards applying biological effects to monitoring.
[So] Source:Mar Environ Res;124:92-105, 2017 Mar.
[Is] ISSN:1879-0291
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological assessment thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the Comet assay, DNA adducts, micronucleus (MN), PAH metabolites, imposex, intersex and fish external pathologies were analysed in four marine sentinel species (Platichthys flesus, Limanda limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and heavy metals were analysed in biota and sediment. Results for sediment and four species in 2008-2009 made it possible to quantify the impact of contaminants using thresholds (Environmental Assessment Criteria/EAC : 70% and EAC : 60%) and effects (EAC : 50% and EAC : 40%) in the Seine estuary. The Seine estuary is ranked among Europe's most highly polluted sites.
[Mh] Termos MeSH primário: Monitoramento Ambiental/métodos
Espécies Sentinelas/metabolismo
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Animais
Baías
Citocromo P-450 CYP1A1/metabolismo
Estuários
Linguado/metabolismo
França
Gastrópodes/metabolismo
Metais Pesados/metabolismo
Mytilus/metabolismo
Bifenilos Policlorados/metabolismo
Hidrocarbonetos Aromáticos Policíclicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals, Heavy); 0 (Polycyclic Aromatic Hydrocarbons); 0 (Water Pollutants, Chemical); DFC2HB4I0K (Polychlorinated Biphenyls); EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170330
[Lr] Data última revisão:
170330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161115
[St] Status:MEDLINE


  6 / 13 MEDLINE  
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[PMID]:27530269
[Au] Autor:Taylor AM; Edge KJ; Ubrihien RP; Maher WA
[Ad] Endereço:Ecochemistry Laboratory, Institute for Applied Ecology, University of Canberra, Canberra, Australian Capital Territory, Australia.
[Ti] Título:The freshwater bivalve Corbicula australis as a sentinel species for metal toxicity assessment: An in situ case study integrating chemical and biomarker analyses.
[So] Source:Environ Toxicol Chem;36(3):709-719, 2017 Mar.
[Is] ISSN:1552-8618
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A weight of evidence approach in environmental assessment includes the use of biomonitor organisms to measure biologically available contaminant concentrations and lethal and sublethal responses in an exposure, dose, and response framework. Corbicula australis was assessed as a test species for metal toxicity using in situ river sediment exposures at 4 locations in the Molonglo River (New South Wales, Australia), which has a legacy of sediment metal contamination, following 8 decades of mining in its upper reaches. A sediment metal contamination gradient was evident from 12.5 km to 47 km downstream of the mine, as follows: zinc (851-130 mg/kg) > lead (104-7 mg/kg) > copper (31-5 mg/kg) > cadmium (2-0.3 mg/kg). Exposed C. australis accumulated the following metals in tissue: zinc (1358-236 µg/g) > copper (24-20 µg/g) > cadmium (4.7-0.7 µg/g) = lead (4.2-1.8 µg/g). Biomarker responses showed increased sublethal impairment with increased tissue metal concentrations. Total antioxidant capacity was mildly impaired, with corresponding increased lipid peroxidation and lysosomal membrane destabilization at the higher tissue metal concentrations. Corbicula australis proved to be an effective biomonitor organism for sediment metal assessment, as it is able to accumulate metals relative to sediment concentrations and showed a pattern of increased sublethal impairment with increased tissue metal concentration. It is recommended as a suitable species for incorporation into local freshwater monitoring and assessment programs. Environ Toxicol Chem 2017;36:709-719. © 2016 SETAC.
[Mh] Termos MeSH primário: Corbicula/efeitos dos fármacos
Monitoramento Ambiental/métodos
Metais Pesados/toxicidade
Rios/química
Espécies Sentinelas
Poluentes Químicos da Água/toxicidade
[Mh] Termos MeSH secundário: Animais
Biomarcadores/metabolismo
Corbicula/metabolismo
Relação Dose-Resposta a Droga
Sedimentos Geológicos/química
Metais Pesados/análise
Metais Pesados/metabolismo
Mineração
New South Wales
Espécies Sentinelas/metabolismo
Poluentes Químicos da Água/análise
Poluentes Químicos da Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Metals, Heavy); 0 (Water Pollutants, Chemical)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160818
[St] Status:MEDLINE
[do] DOI:10.1002/etc.3582


  7 / 13 MEDLINE  
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[PMID]:27368162
[Au] Autor:Dahmani M; Alwassouf S; Grech-Angelini S; Marié JL; Davoust B; Charrel RN
[Ad] Endereço:Research Unit of Emerging Infectious and Tropical Diseases (URMITE) UMR CNRS 7278 IRD 198 INSERM U1015, Aix-Marseille University, Marseille, France. mus.dahmani@gmail.com.
[Ti] Título:Seroprevalence of Toscana virus in dogs from Corsica, France.
[So] Source:Parasit Vectors;9(1):381, 2016 07 01.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Toscana virus (TOSV) is an arbovirus belonging to the Bunyaviridae, a family of negative-stranded, enveloped RNA viruses. The virus can be transmitted to humans through the bite of an infected female sand fly of the genus Phlebotomus. Infections are usually asymptomatic but the virus is known to cause aseptic meningitis and/or meningo-encephalitis in the Mediterranean countries. Dogs are good sentinels for detection of viral circulation and are more easily accessible than wild animals. FINDINGS: In 2013 and 2014, we collected sera from 231 adult dogs living in 26 counties in two departments in Corsica, a French island in the Mediterranean. The virus microneutralization-based seroprevalence assay revealed a seropositivity of 3.9 % dogs on the eastern coast of Corsica. CONCLUSIONS: Our study confirms the circulation of TOSV in Corsica. Accordingly, in geographical areas where dogs possess TOSV neutralizing antibodies, direct and indirect TOSV diagnosis should be implemented in patients presenting with febrile illnesses and central nervous system infections such as meningitis and encephalitis.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Infecções por Bunyaviridae/epidemiologia
Phlebotomus/virologia
Vírus da Febre do Flebótomo Napolitano/imunologia
[Mh] Termos MeSH secundário: Animais
Infecções por Bunyaviridae/virologia
Cães
Feminino
França/epidemiologia
Geografia
Seres Humanos
Ilhas
Masculino
Vírus da Febre do Flebótomo Napolitano/isolamento & purificação
Espécies Sentinelas
Estudos Soroepidemiológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170309
[Lr] Data última revisão:
170309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160703
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-016-1665-4


  8 / 13 MEDLINE  
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[PMID]:27113021
[Au] Autor:Charlotte DR; Yolande BN; Cordonnier S; Claude B
[Ad] Endereço:UMR BOREA CNRS-7208, IRD-207, MNHN, UPMC, UCBN - DYNECAR, Université des Antilles, Campus de Fouillole, BP 592, 97159 Pointe-à-Pitre, Guadeloupe. Electronic address: cdromard@univ-ag.fr.
[Ti] Título:The invasive lionfish, Pterois volitans, used as a sentinel species to assess the organochlorine pollution by chlordecone in Guadeloupe (Lesser Antilles).
[So] Source:Mar Pollut Bull;107(1):102-106, 2016 Jun 15.
[Is] ISSN:1879-3363
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In Guadeloupe, many marine organisms are affected by an organochlorine pollution used in the past by the banana industry to fight against the banana weevil. In the present study, we evaluated the level of contamination of the invasive Indo-Pacific lionfish, Pterois volitans, all around the island. Concentrations of chlordecone varied from 3 to 144µg.kg(-1) wet weight. The highest concentrations were recorded when samples were captured in the marine zones located downstream of the previous banana plantations. This contamination seemed to decrease rapidly with the distance from the coast. Mean concentration of chlordecone in Pterois volitans was higher than that of five other fish species collected in similar sites. Due to its position at the top of the trophic web, lionfish was affected by bioaccumulation of chlordecone and can be used as a sentinel species to assess and control the level of contamination of the marine environment by chlordecone.
[Mh] Termos MeSH primário: Clordecona/análise
Perciformes
Espécies Sentinelas
Poluentes Químicos da Água/análise
[Mh] Termos MeSH secundário: Animais
Guadalupe
Espécies Introduzidas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Water Pollutants, Chemical); RG5XJ88UDF (Chlordecone)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160427
[St] Status:MEDLINE


  9 / 13 MEDLINE  
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[PMID]:27003262
[Au] Autor:Michaels L; Rejmanek D; Aguilar B; Conrad P; Shapiro K
[Ad] Endereço:Pathology, Microbiology and Immunology Department,School of Veterinary Medicine, University of California Davis,One Shields Avenue,Davis, CA 95616,USA.
[Ti] Título:California mussels (Mytilus californianus) as sentinels for marine contamination with Sarcocystis neurona.
[So] Source:Parasitology;143(6):762-9, 2016 05.
[Is] ISSN:1469-8161
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sarcocystis neurona is a terrestrial parasite that can cause fatal encephalitis in the endangered Southern sea otter (Enhydra lutris nereis). To date, neither risk factors associated with marine contamination nor the route of S. neurona infection to marine mammals has been described. This study evaluated coastal S. neurona contamination using California mussels (Mytilus californianus) as sentinels for pathogen pollution. A field investigation was designed to test the hypotheses that (1) mussels can serve as sentinels for S. neurona contamination, and (2) S. neurona contamination in mussels would be highest during the rainy season and in mussels collected near freshwater. Initial validation of molecular assays through sporocyst spiking experiments revealed the ITS-1500 assay to be most sensitive for detection of S. neurona, consistently yielding parasite amplification at concentrations ⩾5 sporocysts/1 mL mussel haemolymph. Assays were then applied on 959 wild-caught mussels, with detection of S. neurona confirmed using sequence analysis in three mussels. Validated molecular assays for S. neurona detection in mussels provide a novel toolset for investigating marine contamination with this parasite, while confirmation of S. neurona in wild mussels suggests that uptake by invertebrates may serve as a route of transmission to susceptible marine animals.
[Mh] Termos MeSH primário: Monitoramento Ambiental/métodos
Mytilus/parasitologia
Sarcocystis/fisiologia
Espécies Sentinelas/parasitologia
[Mh] Termos MeSH secundário: Animais
California
Reprodutibilidade dos Testes
Estações do Ano
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170407
[Lr] Data última revisão:
170407
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160323
[St] Status:MEDLINE
[do] DOI:10.1017/S0031182016000354


  10 / 13 MEDLINE  
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[PMID]:26994836
[Au] Autor:Pippy BA; Kidd KA; Munkittrick KR; Mercer A; Hunt H
[Ad] Endereço:Canadian Rivers Institute and Department of Biology, University of New Brunswick, Saint John, New Brunswick E2L 4L5, Canada.
[Ti] Título:Use of the Atlantic nut clam (Nucula proxima) and catworm (Nephtys incisa) in a sentinel species approach for monitoring the health of Bay of Fundy estuaries.
[So] Source:Mar Pollut Bull;106(1-2):225-35, 2016 May 15.
[Is] ISSN:1879-3363
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Designing an effective environmental monitoring system for population responses requires knowledge of the biology of appropriate sentinel species and baseline information on the area's physical and chemical characteristics. This study collected information in Saint John Harbor, NB, Canada, for two abundant marine benthic invertebrates, the Atlantic nut clam (Nucula proxima) and the catworm (Nephtys incisa) to characterize their seasonal and spatial variability, determine the ideal sampling time and methods, and develop baseline data for future studies. We also evaluated whether contamination is impacting invertebrates by comparing sediment metal concentrations to responses of benthic infauna. Metals were generally below sediment quality guidelines except for nickel and arsenic. Clam densities were variable between sites but not seasons, whereas catworm densities were not significantly different between sites or seasons. Overall, these species show potential for environmental monitoring, although investigation at more contaminated sites is warranted to assess their sensitivity.
[Mh] Termos MeSH primário: Bivalves/metabolismo
Monitoramento Ambiental/métodos
Poliquetos/metabolismo
Poluentes Químicos da Água/metabolismo
[Mh] Termos MeSH secundário: Animais
Organismos Aquáticos
Arsênico
Baías
Canadá
Estuários
Sedimentos Geológicos/química
Invertebrados
Metais
Estações do Ano
Espécies Sentinelas
Frutos do Mar
Poluentes Químicos da Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals); 0 (Water Pollutants, Chemical); N712M78A8G (Arsenic)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160321
[St] Status:MEDLINE



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