Base de dados : MEDLINE
Pesquisa : C01.252.400.054.160.400 [Categoria DeCS]
Referências encontradas : 442 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 45 ir para página                         

  1 / 442 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28988038
[Au] Autor:Nefefe T; Liebenberg J; van Kleef M; Steyn HC; Pretorius A
[Ad] Endereço:Agricultural Research Council - Onderstepoort Veterinary Research, Private Bag X5, Onderstepoort, 0110, South Africa; Department of Veterinary Tropical Diseases, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa. Electronic address: NefefeT@arc.agric.za.
[Ti] Título:Innate immune transcriptomic evaluation of PBMC isolated from sheep after infection with E. ruminantium Welgevonden strain.
[So] Source:Mol Immunol;91:238-248, 2017 11.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Heartwater is a tick-borne non-infectious fatal disease of wild and domestic ruminants caused by the bacterium Ehrlichia ruminantium, transmitted by Amblyomma ticks. Although there is evidence that interferon-gamma (IFN-γ) controls E. ruminantium growth and that cellular immune responses could be protective, an effective recombinant vaccine for this disease is lacking. An overall analysis of which immune pathways are up- or down-regulated in sheep peripheral blood mononuclear cells is expected to lead to a better understanding of the global immune response of sheep to E. ruminantium infection. Therefore, a systems biology oriented approach following the infection with E. ruminantium was investigated from peripheral blood mononuclear cells to aid recombinant vaccine development. In this study, heartwater naïve sheep were infected and challenged by allowing E. ruminantium infected ticks to feed on them. After primary infection, all the animals were treated with antibiotic during the resulting febrile response. Blood was collected daily for E. ruminantium detection by qPCR (pCS20 assay). The pCS20 assay only detected the pathogen in the blood one day prior to and during the febrile stage of infection confirming infection of the sheep. IFN-γ real-time PCR indicated that this cytokine was expressed at specific time points: post infection, during the febrile stage of the disease and after challenge. These were used as a guide to select samples for transcriptome sequencing. This paper focuses on transcripts that are associated with innate activating pathways that were identified to be up- and down-regulated after primary infection and the subsequent challenge. These included the CD14 monocyte marker, toll-like receptor (TLR), nod-like receptor, chemokine, cytosolic and cytokine-cytokine interaction receptor pathways. In particular, TLR4, TLR9 and CD14 were activated together with DNA detection pathways, suggesting that vaccine formulations may be improved if CpG motifs and lipopolysaccharides are included. This data indicates that innate immune activation, perhaps by using adjuvants, should be an important component for consideration during future heartwater recombinant vaccine development.
[Mh] Termos MeSH primário: Ehrlichia ruminantium/imunologia
Hidropericárdio/imunologia
Imunidade Inata
Leucócitos Mononucleares/imunologia
Doenças dos Ovinos/imunologia
Ovinos/imunologia
Transcriptoma/imunologia
[Mh] Termos MeSH secundário: Animais
Feminino
Hidropericárdio/patologia
Leucócitos Mononucleares/patologia
Masculino
Ovinos/microbiologia
Doenças dos Ovinos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171009
[St] Status:MEDLINE


  2 / 442 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27747194
[Au] Autor:Cangi N; Gordon JL; Bournez L; Pinarello V; Aprelon R; Huber K; Lefrançois T; Neves L; Meyer DF; Vachiéry N
[Ad] Endereço:CIRAD, UMR CMAEEPetit-Bourg, Guadeloupe, France; INRA, UMR1309 CMAEEMontpellier, France; Centro de Biotecnologia-UEM, Eduardo Mondlane UniversityMaputo, Mozambique; Université des AntillesPointe-à-Pitre, Guadeloupe, France.
[Ti] Título:Recombination Is a Major Driving Force of Genetic Diversity in the Anaplasmataceae .
[So] Source:Front Cell Infect Microbiol;6:111, 2016.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The disease, Heartwater, caused by the , represents a major problem for tropical livestock and wild ruminants. Up to now, no effective vaccine has been available due to a limited cross protection of vaccinal strains on field strains and a high genetic diversity of within geographical locations. To address this issue, we inferred the genetic diversity and population structure of 194 isolates circulating worldwide using Multilocus Sequence Typing based on , and . Phylogenetic trees and networks were generated using BEAST and SplitsTree, respectively, and recombination between the different genetic groups was tested using the PHI test for recombination. Our study reveals the repeated occurrence of recombination between strains, suggesting that it may occur frequently in the genome and has likely played an important role in the maintenance of genetic diversity and the evolution of . Despite the unclear phylogeny and phylogeography, isolates are clustered into two main groups: Group 1 (West Africa) and a Group 2 (worldwide) which is represented by West, East, and Southern Africa, Indian Ocean, and Caribbean strains. Some sequence types are common between West Africa and Caribbean and between Southern Africa and Indian Ocean strains. These common sequence types highlight two main introduction events due to the movement of cattle: from West Africa to Caribbean and from Southern Africa to the Indian Ocean islands. Due to the long branch lengths between Group 1 and Group 2, and the propensity for recombination between these groups, it seems that the West African clusters of Subgroup 2 arrived there more recently than the original divergence of the two groups, possibly with the original waves of domesticated ruminants that spread across the African continent several thousand years ago.
[Mh] Termos MeSH primário: Anaplasmataceae/genética
Ehrlichia ruminantium/genética
Evolução Molecular
Variação Genética/genética
Genótipo
Recombinação Genética
[Mh] Termos MeSH secundário: África/epidemiologia
Animais
Proteínas de Bactérias/genética
Sequência de Bases
Bovinos
Doenças dos Bovinos/epidemiologia
Doenças dos Bovinos/microbiologia
DNA Bacteriano
Ehrlichia ruminantium/classificação
Ehrlichia ruminantium/isolamento & purificação
Ehrlichia ruminantium/patogenicidade
Hidropericárdio/sangue
Hidropericárdio/epidemiologia
Hidropericárdio/microbiologia
Ilhas do Oceano Índico/epidemiologia
Moçambique/epidemiologia
Tipagem de Sequências Multilocus/métodos
Filogenia
Reação em Cadeia da Polimerase/métodos
Superóxido Dismutase/genética
Carrapatos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (DNA, Bacterial); 0 (LipA protein, Bacteria); 0 (lipase activator protein, Bacteria); EC 1.15.1.1 (SodB protein, Bacteria); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161018
[St] Status:MEDLINE


  3 / 442 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:27610355
[Au] Autor:Tago D; Meyer DF
[Ad] Endereço:La Recherche Agronomique Pour le Développement (CIRAD), UMR Contrôle des Maladies Animales, Exotiques et Émergentes (CMAEE)Petit-Bourg, France; Institut National de la Recherche Agronomique, UMR1309 CMAEEMontpellier, France.
[Ti] Título:Economic Game Theory to Model the Attenuation of Virulence of an Obligate Intracellular Bacterium.
[So] Source:Front Cell Infect Microbiol;6:86, 2016.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Diseases induced by obligate intracellular pathogens have a large burden on global human and animal health. Understanding the factors involved in the virulence and fitness of these pathogens contributes to the development of control strategies against these diseases. Based on biological observations, a theoretical model using game theory is proposed to explain how obligate intracellular bacteria interact with their host. The equilibrium in such a game shows that the virulence and fitness of the bacterium is host-triggered and by changing the host's defense system to which the bacterium is confronted, an evolutionary process leads to an attenuated strain. Although, the attenuation procedure has already been conducted in practice in order to develop an attenuated vaccine (e.g., with Ehrlichia ruminantium), there was a lack of understanding of the theoretical basis behind this process. Our work provides a model to better comprehend the existence of different phenotypes and some underlying evolutionary mechanisms for the virulence of obligate intracellular bacteria.
[Mh] Termos MeSH primário: Bactérias/imunologia
Bactérias/patogenicidade
Vacinas Bacterianas/imunologia
Teoria do Jogo
Modelos Biológicos
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
Citoplasma/microbiologia
Ehrlichia ruminantium/imunologia
Ehrlichia ruminantium/patogenicidade
Hidropericárdio/imunologia
Hidropericárdio/prevenção & controle
Interações Hospedeiro-Patógeno
Seres Humanos
Vacinas Atenuadas/imunologia
Virulência/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Vaccines); 0 (Vaccines, Attenuated)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160910
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2016.00086


  4 / 442 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27608502
[Au] Autor:Thema N; Pretorius A; Tshilwane SI; Liebenberg J; Steyn H; Van Kleef M
[Ad] Endereço:New Generation Vaccines Programme, Agricultural Research Council-Onderstepoort Veterinary Institute; Department of Veterinary Tropical Diseases, University of Pretoria. theman@arc.agric.za.
[Ti] Título:Cellular immune responses induced in vitro by Ehrlichia ruminantium secreted proteins and identification of vaccine candidate peptides.
[So] Source:Onderstepoort J Vet Res;83(1):e1-e11, 2016 Aug 30.
[Is] ISSN:2219-0635
[Cp] País de publicação:South Africa
[La] Idioma:eng
[Ab] Resumo:Secreted proteins are reported to induce cell-mediated immunity characterised by the production of interferon-gamma (IFN)-γ. In this study three open reading frames (ORFs) (Erum8060, Erum7760, Erum5000) encoding secreted proteins were selected from the Ehrlichia ruminantium (Welgevonden) genome sequence using bioinformatics tools to determine whether they induce a cellular immune response in vitro with mononuclear cells from needle and tick infected animals. The whole recombinant protein of the three ORFs as well as four adjacent fragments of the Erum5000 protein (Erum5000A, Erum5000B, Erum5000C, Erum5000D) were successfully expressed in a bacterial expression system which was confirmed by immunoblots using anti-His antibodies and sheep sera. These recombinant proteins were assayed with immune sheep and cattle peripheral blood mononuclear cells (PBMCs), spleen and lymph node (LN) cells to determine whether they induce recall cellular immune responses in vitro. Significant proliferative responses and IFN-γ production were evident for all recombinant proteins, especially Erum5000A, in both ruminant species tested. Thus overlapping peptides spanning Erum5000A were synthesised and peptides that induce proliferation of memory CD4+ and CD8+ T cells and production of IFN-γ were identified. These results illustrate that a Th1 type immune response was elicited and these recombinant proteins and peptides may therefore be promising candidates for development of a heartwater vaccine.
[Mh] Termos MeSH primário: Proteínas de Bactérias/genética
Vacinas Bacterianas/imunologia
Ehrlichia ruminantium/imunologia
Hidropericárdio/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Bovinos
Ehrlichia ruminantium/genética
Imunização/veterinária
Interferon gama/biossíntese
Ativação Linfocitária
Fases de Leitura Aberta
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Bacterial Vaccines); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160910
[St] Status:MEDLINE
[do] DOI:10.4102/ojvr.v83i1.1170


  5 / 442 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26829905
[Au] Autor:Mdladla K; Dzomba EF; Muchadeyi FC
[Ad] Endereço:Agricultural Research Council, Biotechnology Platform, Private Bag X5, Onderstepoort 0110, South Africa; Discipline of Genetics, School of Life Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville 3209, South Africa. Electronic address: MdladlaK@arc.agric.za.
[Ti] Título:Seroprevalence of Ehrlichia ruminantium antibodies and its associated risk factors in indigenous goats of South Africa.
[So] Source:Prev Vet Med;125:99-105, 2016 Mar 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The present study investigated the seroprevalence of antibodies to Ehrlichia ruminantium and the associated risk factors in goats from five different farming provinces of South Africa. Sera collected from 686 goats of the commercial meat type (n=179), mohair type (n=9), non-descript indigenous goats from Eastern Cape (n=56), KwaZulu-Natal (n=209), Limpopo (n=111), North West (n=61) and Northern Cape (n=11) provinces and a feral Tankwa goat (n=50) were tested for the presence of immunoglobulin G (IgG) antibodies to antigens of E. ruminantium using the indirect fluorescent-antibody test (IFAT). Fifty two percent of these goats had ticks. The overall seroprevalence of antibodies to E. ruminantium was 64.87% (445/686) with the highest seroprevalence reported for Limpopo (95.50%) and lowest for Northern Cape (20.29%). Highest seroprevalence for antibodies to E. ruminantium was observed in goats from endemic regions (76.09%), and from smallholder production systems (89.54%). High seroprevalence was also observed in non-descript indigenous goats (85.04%), adult goat (69.62%), in does (67.46%) and goats infested with ticks (85.79%). The logistic model showed a gradient of increasing risk for commercial meat type Savanna (OR=3.681; CI=1.335-10.149) and non-descript indigenous (OR=3.466; CI=1.57-7.645) compared to Boer goats and for goats from the smallholder production system (OR=2.582; CI=1.182-5.639) and those with ticks (OR=3.587; CI=2.105-6.112). Results from this study showed that E. ruminantium infections were prevalent but were widely and unevenly distributed throughout South Africa. Findings from the study facilitate identification and mapping of risk areas for heartwater and its endeminicity in South Africa and should be taken into consideration for future disease control strategies and local goat improvement programs.
[Mh] Termos MeSH primário: Criação de Animais Domésticos/métodos
Ehrlichia ruminantium/isolamento & purificação
Doenças das Cabras/epidemiologia
Hidropericárdio/epidemiologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antibacterianos/sangue
Feminino
Geografia
Doenças das Cabras/microbiologia
Cabras
Hidropericárdio/microbiologia
Masculino
Fatores de Risco
Estudos Soroepidemiológicos
África do Sul/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Bacterial)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160203
[St] Status:MEDLINE


  6 / 442 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26348980
[Au] Autor:Pompo K; Mays S; Wesselman C; Paulsen DJ; Fryxell RT
[Ad] Endereço:370 Plant Biotechnology Building, 2505 E J Chapman Drive, Department of Entomology and Plant Pathology, University of Tennessee Institute of Agriculture, Knoxville, Tennessee 37996-4560.
[Ti] Título:Survey of Ticks Collected from Tennessee Cattle and Their Pastures for Anaplasma and Ehrlichia Species.
[So] Source:J Parasitol;102(1):54-9, 2016 Feb.
[Is] ISSN:1937-2345
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Anaplasma marginale is the causative agent for bovine anaplasmosis (BA) and Ehrlichia ruminantium is the causative agent for heartwater, 2 devastating diseases of cattle. BA is common in the United States and frequently reported in western Tennessee cattle; however, cases of heartwater are not yet established in the continental United States. Because both pathogens are transmitted via the bites of infected ticks, the objective of this study was to survey cattle and pastures for ticks and for each pathogen. University of Tennessee AgResearch has 7 research and education centers (REC) located throughout the state at which they manage cattle. Ticks were collected from selected cattle (every fourth to sixth animal) and pastures (via dragging) associated with the herd from each REC during the summer of 2013. A total of 512 ticks were collected from cattle (n = 386) and pastures (n = 126) and were PCR-screened for Anaplasma and Ehrlichia using genus-specific primers. Collections consisted of 398 (77.7%) Amblyomma americanum, 84 (16.4%) Amblyomma maculatum, and 30 (5.9%) Dermacentor variabilis. Ticks were not recovered from pastures or cattle east of the Tennessee Plateau. The North American vectors for An. marginale and E. ruminantium were identified (D. variabilis and A. maculatum, respectively), but neither pathogen was recovered. A large proportion of ticks were collected from cattle and, of these, a majority were attached to their host (compared to questing on their host or engorged on the host). Four A. americanum were positive for Ehrlichia spp. (Ehrlichia ewingii, Ehrlichia chaffeensis, and Panola Mountain Ehrlichia), all in western Tennessee. With the identification of a few Ehrlichia infections in cattle-associated ticks and current A. marginale rates in Tennessee beef cattle nearing 11%, additional research is needed to establish baseline tick, Anaplasma, and Ehrlichia data for future management studies.
[Mh] Termos MeSH primário: Anaplasma/isolamento & purificação
Vetores Aracnídeos/microbiologia
Doenças dos Bovinos/parasitologia
Ehrlichia/isolamento & purificação
Infestações por Carrapato/veterinária
Carrapatos/microbiologia
[Mh] Termos MeSH secundário: Anaplasmose/transmissão
Animais
Bovinos
Doenças dos Bovinos/microbiologia
Doenças dos Bovinos/transmissão
Feminino
Hidropericárdio/transmissão
Masculino
Tennessee
Infestações por Carrapato/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1607
[Cu] Atualização por classe:160218
[Lr] Data última revisão:
160218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150909
[St] Status:MEDLINE
[do] DOI:10.1645/15-814


  7 / 442 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25807955
[Au] Autor:Sayler KA; Loftis AD; Mahan SM; Barbet AF
[Ad] Endereço:Department of Physiological Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA. saylerk@ufl.edu.
[Ti] Título:Development of a Quantitative PCR Assay for Differentiating the Agent of Heartwater Disease, Ehrlichia ruminantium, from the Panola Mountain Ehrlichia.
[So] Source:Transbound Emerg Dis;63(6):e260-e269, 2016 Dec.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Panola Mountain Ehrlichia (PME) is an emerging Ehrlichia sp. reported in ten US states. Based on the sequence homology of all known genes, PME is closely related to Ehrlichia ruminantium (ER), the causative agent of heartwater. Heartwater is an economically important tick-borne disease of cattle, sheep and goats responsible for stock losses in sub-Saharan Africa. Unfortunately, ER was imported to the Caribbean islands in the 19th century, and the presence of this foreign animal disease in the Caribbean poses a threat to the US mainland. If introduced, a heartwater outbreak would cause massive losses of naïve livestock. The serologic assay of choice to diagnose heartwater is cross-reactive with Ehrlichia spp., including PME, as we demonstrate here, which would confound disease surveillance in the event of a heartwater outbreak. The purpose of this study was to develop a diagnostic assay capable of rapidly distinguishing between these pathogens. Using synthetic MAP-1B peptides for ER and PME, we tested the cross-reactivity of this assay using sera from infected livestock. The MAP-1B ELISA cannot distinguish between animals infected with PME and ER. Therefore, a dual-plex Taqman qPCR assay targeting the groEL gene of PME and ER was developed and validated. Primers were designed that are conserved among all known strains of ER, allowing for the amplification of strains from the Caribbean and Africa. The assay is highly sensitive (10 copies of DNA) and specific. This assay distinguishes between infection with PME and ER and will be a valuable tool in the event of heartwater outbreak on the US mainland, or for epidemiological studies involving either disease-causing organism.
[Mh] Termos MeSH primário: Ehrlichia ruminantium/genética
Ehrlichia ruminantium/isolamento & purificação
Ehrlichia/genética
Ehrlichia/isolamento & purificação
Hidropericárdio/diagnóstico
Reação em Cadeia da Polimerase em Tempo Real
[Mh] Termos MeSH secundário: África
Animais
Bovinos
Doenças dos Bovinos/epidemiologia
Primers do DNA
Ensaio de Imunoadsorção Enzimática
Cabras
Hidropericárdio/epidemiologia
Ovinos
Estados Unidos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150327
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12339


  8 / 442 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:26601456
[Au] Autor:Allsopp BA
[Ti] Título:Heartwater--Ehrlichia ruminantium infection.
[So] Source:Rev Sci Tech;34(2):557-68, 2015 Aug.
[Is] ISSN:0253-1933
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Summary Heartwater is a notifiable disease that is listed by the World Organisation for Animal Health. It is caused by Ehrlichia ruminantium, an obligately intracellular Gram-negative bacterium in the order Rickettsiales and the family Anaplasmataceae. The disease is borne byticks in the genus Amblyomma and causes heartwater, or cowdriosis, in wild and domestic ruminants, primarily in Africa, but also in parts of the Caribbean. The disease was recognised in South Africa in the 19th Century and determined to be tick borne in 1900, while the organism was identified in 1925 and first cultured in vitro in 1985. This latter achievement boosted research into the disease at a time when biology was moving into the molecular genetic age. Over the last 20 years, there have been significant improvements in our understanding of E. ruminantium, yielding major advances in diagnosis, epidemiology, genetic characterisation, phylogeny, immunology, and vaccine development. The organism is genetically highly variable; this has important implications for future control measures, and is making it difficult to develop an effective vaccine for protection against tick challenge. Research is continuing into three different types of vaccine, inactivated, attenuated, and recombinant, and the current state of development of each is discussed.
[Mh] Termos MeSH primário: Ehrlichia ruminantium
Hidropericárdio/microbiologia
[Mh] Termos MeSH secundário: Animais
Vacinas Bacterianas/imunologia
Ehrlichia ruminantium/genética
Genótipo
Saúde Global
Hidropericárdio/epidemiologia
Hidropericárdio/prevenção & controle
Carrapatos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bacterial Vaccines)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:151125
[Lr] Data última revisão:
151125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151126
[St] Status:MEDLINE


  9 / 442 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25514207
[Au] Autor:Marcelino I; Lefrançois T; Martinez D; Giraud-Girard K; Aprelon R; Mandonnet N; Gaucheron J; Bertrand F; Vachiéry N
[Ad] Endereço:CIRAD, UMR CMAEE, F-97170 Petit-Bourg, Guadeloupe, France. Electronic address: isabel.marcelino@cirad.fr.
[Ti] Título:A user-friendly and scalable process to prepare a ready-to-use inactivated vaccine: the example of heartwater in ruminants under tropical conditions.
[So] Source:Vaccine;33(5):678-85, 2015 Jan 29.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The use of cheap and thermoresistant vaccines in poor tropical countries for the control of animal diseases is a key issue. Our work aimed at designing and validating a process for the large-scale production of a ready-to-use inactivated vaccine for ruminants. Our model was heartwater caused by the obligate intracellular bacterium Ehrlichia ruminantium (ER). The conventional inactivated vaccine against heartwater (based on whole bacteria inactivated with sodium azide) is prepared immediately before injection, using a syringe-extrusion method with Montanide ISA50. This is a fastidious time-consuming process and it limits the number of vaccine doses available. To overcome these issues, we tested three different techniques (syringe, vortex and homogenizer) and three Montanide ISA adjuvants (50, 70 and 70M). High-speed homogenizer was the optimal method to emulsify ER antigens with both ISA70 and 70M adjuvants. The emulsions displayed a good homogeneity (particle size below 1 µm and low phase separation), conductivity below 10 µS/cm and low antigen degradation at 4 °C for up to 1 year. The efficacy of the different formulations was then evaluated during vaccination trials on goats. The inactivated ER antigens emulsified with ISA70 and ISA70M in a homogenizer resulted in 80% and 100% survival rates, respectively. A cold-chain rupture assay using ISA70M+ER was performed to mimic possible field conditions exposing the vaccine at 37 °C for 4 days before delivery. Surprisingly, the animal survival rate was still high (80%). We also observed that the MAP-1B antibody response was very similar between animals vaccinated with ISA70+ER and ISA70M+ER emulsions, suggesting a more homogenous antigen distribution and presentation in these emulsions. Our work demonstrated that the combination of ISA70 or ISA70M and homogenizer is optimal for the production of an effective ready-to-use inactivated vaccine against heartwater, which could easily be produced on an industrial scale.
[Mh] Termos MeSH primário: Vacinas Bacterianas/imunologia
Vacinas Bacterianas/isolamento & purificação
Ehrlichia ruminantium/imunologia
Hidropericárdio/prevenção & controle
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/administração & dosagem
Animais
Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/imunologia
Vacinas Bacterianas/administração & dosagem
Estabilidade de Medicamentos
Emulsões/administração & dosagem
Emulsões/isolamento & purificação
Cabras
Hidropericárdio/imunologia
Injeções Intramusculares
Análise de Sobrevida
Clima Tropical
Vacinação/métodos
Vacinas de Produtos Inativados/administração & dosagem
Vacinas de Produtos Inativados/imunologia
Vacinas de Produtos Inativados/isolamento & purificação
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Bacterial); 0 (Antigens, Bacterial); 0 (Bacterial Vaccines); 0 (Emulsions); 0 (Vaccines, Inactivated)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:150123
[Lr] Data última revisão:
150123
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141217
[St] Status:MEDLINE


  10 / 442 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:24685441
[Au] Autor:Moniuszko A; Rückert C; Alberdi MP; Barry G; Stevenson B; Fazakerley JK; Kohl A; Bell-Sakyi L
[Ad] Endereço:The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, Scotland, UK; Department of Infectious Diseases and Neuroinfections, Medical University in Bialystok, Zurawia 14, 15-540 Bialystok, Poland.
[Ti] Título:Coinfection of tick cell lines has variable effects on replication of intracellular bacterial and viral pathogens.
[So] Source:Ticks Tick Borne Dis;5(4):415-22, 2014 Jun.
[Is] ISSN:1877-9603
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Ticks transmit various human and animal microbial pathogens and may harbour more than one pathogen simultaneously. Both viruses and bacteria can trigger, and may subsequently suppress, vertebrate host and arthropod vector anti-microbial responses. Microbial coinfection of ticks could lead to an advantage or disadvantage for one or more of the microorganisms. In this preliminary study, cell lines derived from the ticks Ixodes scapularis and Ixodes ricinus were infected sequentially with 2 arthropod-borne pathogens, Borrelia burgdorferi s.s., Ehrlichia ruminantium, or Semliki Forest virus (SFV), and the effect of coinfection on the replication of these pathogens was measured. Prior infection of tick cell cultures with the spirochaete B. burgdorferi enhanced subsequent replication of the rickettsial pathogen E. ruminantium whereas addition of spirochaetes to cells infected with E. ruminantium had no effect on growth of the latter. Both prior and subsequent presence of B. burgdorferi also had a positive effect on SFV replication. Presence of E. ruminantium or SFV had no measurable effect on B. burgdorferi growth. In tick cells infected first with E. ruminantium and then with SFV, virus replication was significantly higher across all time points measured (24, 48, 72h post infection), while presence of the virus had no detectable effect on bacterial growth. When cells were infected first with SFV and then with E. ruminantium, there was no effect on replication of either pathogen. The results of this preliminary study indicate that interplay does occur between different pathogens during infection of tick cells. Further study is needed to determine if this results from direct pathogen-pathogen interaction or from effects on host cell defences, and to determine if these observations also apply in vivo in ticks. If presence of one pathogen in the tick vector results in increased replication of another, this could have implications for disease transmission and incidence.
[Mh] Termos MeSH primário: Infecções por Alphavirus/transmissão
Borrelia burgdorferi/fisiologia
Ehrlichia ruminantium/fisiologia
Hidropericárdio/transmissão
Ixodes/microbiologia
Doença de Lyme/transmissão
Vírus da Floresta de Semliki/fisiologia
[Mh] Termos MeSH secundário: Infecções por Alphavirus/virologia
Animais
Linhagem Celular
Coinfecção
Genes Reporter
Hidropericárdio/microbiologia
Seres Humanos
Doença de Lyme/microbiologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1504
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140402
[St] Status:MEDLINE



página 1 de 45 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde