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[PMID]:28542807
[Au] Autor:Thong KL; Tan LK; Ooi PT
[Ad] Endereço:Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
[Ti] Título:Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia.
[So] Source:J Sci Food Agric;98(1):87-95, 2018 Jan.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The objectives of the present study were to determine the antimicrobial resistance, virulotypes and genetic diversity of Yersinia enterocolitica isolated from uncooked porcine food and live pigs in Malaysia. RESULTS: Thirty-two non-repeat Y. enterocolitica strains of three bioserotypes (3 variant/O:3, n = 27; 1B/O:8, n = 3; 1A/O:5, n = 2) were analysed. Approximately 90% of strains were multidrug-resistant with a multiple antibiotic resistance index < 0.2 and the majority of the strains were resistant to nalidixic acid, clindamycin, ampicillin, ticarcillin, tetracycline and amoxicillin. Yersinia enterocolitica could be distinguished distinctly into three clusters by pulsed-field gel electrophoresis, with each belonging to a particular bioserotype. Strains of 3 variant/O:3 were more heterogeneous than others. Eleven of the 15 virulence genes tested (hreP, virF, rfbC, myfA, sat, inv, ail, ymoA, ystA, tccC, yadA) and pYV virulence plasmid were present in all the bioserotpe 3 variant/03 strains. CONCLUSION: The occurrence of virulent strains of Y. enterocolitica in pigs and porcine products reiterated that pigs are important reservoirs for Y. enterocolitica. The increasing trend of multidrug resistant strains is a public health concern. This is the first report on the occurrence of potential pathogenic and resistant strains of Y. enterocolitica in pigs in Malaysia. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Produtos da Carne/microbiologia
Doenças dos Suínos/microbiologia
Yersiniose/veterinária
Yersinia enterocolitica/efeitos dos fármacos
Yersinia enterocolitica/genética
[Mh] Termos MeSH secundário: Animais
Farmacorresistência Bacteriana
Variação Genética
Malásia
Produtos da Carne/análise
Suínos
Virulência
Yersiniose/microbiologia
Yersinia enterocolitica/isolamento & purificação
Yersinia enterocolitica/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180102
[Lr] Data última revisão:
180102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8442


  2 / 3245 MEDLINE  
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[PMID]:28920954
[Au] Autor:Menon MB; Gropengießer J; Fischer J; Novikova L; Deuretzbacher A; Lafera J; Schimmeck H; Czymmeck N; Ronkina N; Kotlyarov A; Aepfelbacher M; Gaestel M; Ruckdeschel K
[Ad] Endereço:Institute of Cell Biochemistry, Hannover Medical School, Hannover 30625, Germany.
[Ti] Título:p38 /MK2-dependent phosphorylation controls cytotoxic RIPK1 signalling in inflammation and infection.
[So] Source:Nat Cell Biol;19(10):1248-1259, 2017 Oct.
[Is] ISSN:1476-4679
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Receptor-interacting protein kinase-1 (RIPK1), a master regulator of cell fate decisions, was identified as a direct substrate of MAPKAP kinase-2 (MK2) by phosphoproteomic screens using LPS-treated macrophages and stress-stimulated embryonic fibroblasts. p38 /MK2 interact with RIPK1 in a cytoplasmic complex and MK2 phosphorylates mouse RIPK1 at Ser321/336 in response to pro-inflammatory stimuli, such as TNF and LPS, and infection with the pathogen Yersinia enterocolitica. MK2 phosphorylation inhibits RIPK1 autophosphorylation, curtails RIPK1 integration into cytoplasmic cytotoxic complexes, and suppresses RIPK1-dependent apoptosis and necroptosis. In Yersinia-infected macrophages, RIPK1 phosphorylation by MK2 protects against infection-induced apoptosis, a process targeted by Yersinia outer protein P (YopP). YopP suppresses p38 /MK2 activation to increase Yersinia-driven apoptosis. Hence, MK2 phosphorylation of RIPK1 is a crucial checkpoint for cell fate in inflammation and infection that determines the outcome of bacteria-host cell interaction.
[Mh] Termos MeSH primário: Apoptose
Inflamação/enzimologia
Peptídeos e Proteínas de Sinalização Intracelular/metabolismo
Macrófagos/enzimologia
Proteínas Serina-Treonina Quinases/metabolismo
Proteína Serina-Treonina Quinases de Interação com Receptores/metabolismo
Yersiniose/enzimologia
Yersinia enterocolitica/patogenicidade
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Proteínas de Bactérias/metabolismo
Citosol/enzimologia
Citosol/microbiologia
Feminino
Genótipo
Células HEK293
Interações Hospedeiro-Patógeno
Seres Humanos
Quinase I-kappa B/metabolismo
Inflamação/patologia
Peptídeos e Proteínas de Sinalização Intracelular/deficiência
Peptídeos e Proteínas de Sinalização Intracelular/genética
MAP Quinase Quinase Quinases/metabolismo
Macrófagos/efeitos dos fármacos
Macrófagos/microbiologia
Macrófagos/patologia
Masculino
Proteínas de Membrana/metabolismo
Camundongos Knockout
Necrose
Fenótipo
Fosforilação
Proteínas Serina-Treonina Quinases/deficiência
Proteínas Serina-Treonina Quinases/genética
Proteína Serina-Treonina Quinases de Interação com Receptores/genética
Receptores Tipo I de Fatores de Necrose Tumoral/genética
Receptores Tipo I de Fatores de Necrose Tumoral/metabolismo
Serina
Transdução de Sinais
Fatores de Tempo
Transfecção
Fator de Necrose Tumoral alfa/toxicidade
Yersiniose/microbiologia
Yersiniose/patologia
Yersinia enterocolitica/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Intracellular Signaling Peptides and Proteins); 0 (Membrane Proteins); 0 (Receptors, Tumor Necrosis Factor, Type I); 0 (Tnfrsf1a protein, mouse); 0 (Tumor Necrosis Factor-alpha); 0 (Yop proteins translocation protein P, Yersinia); 452VLY9402 (Serine); EC 2.7.1.- (MAP-kinase-activated kinase 2); EC 2.7.1.- (MAP-kinase-activated kinase 3); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (Receptor-Interacting Protein Serine-Threonine Kinases); EC 2.7.11.1 (Ripk1 protein, mouse); EC 2.7.11.10 (I-kappa B Kinase); EC 2.7.11.10 (Ikbkb protein, mouse); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases); EC 2.7.11.25 (MAP Kinase Kinase Kinases); EC 2.7.11.25 (MAP kinase kinase kinase 7)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.1038/ncb3614


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[PMID]:28716827
[Au] Autor:Davicino RC; Méndez-Huergo SP; Eliçabe RJ; Stupirski JC; Autenrieth I; Di Genaro MS; Rabinovich GA
[Ad] Endereço:División de Inmunología, Facultad de Química, Bioquímica y Farmacia, Universidad Nacional de San Luis e Instituto Multidisciplinario de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas, C5700 San Luis, Argentina.
[Ti] Título:Galectin-1-Driven Tolerogenic Programs Aggravate Infection by Repressing Antibacterial Immunity.
[So] Source:J Immunol;199(4):1382-1392, 2017 Aug 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:is an enteropathogenic bacterium that causes gastrointestinal disorders, as well as extraintestinal manifestations. To subvert the host's immune response, uses a type III secretion system consisting of an injectisome and effector proteins, called outer proteins (Yops), that modulate activation, signaling, and survival of immune cells. In this article, we show that galectin-1 (Gal-1), an immunoregulatory lectin widely expressed in mucosal tissues, contributes to pathogenicity by undermining protective antibacterial responses. We found higher expression of Gal-1 in the spleen and Peyer's patches of mice infected orogastrically with serotype O:8 compared with noninfected hosts. This effect was prevented when mice were infected with lacking YopP or YopH, two critical effectors involved in bacterial immune evasion. Consistent with a regulatory role for this lectin during pathogenesis, mice lacking Gal-1 showed increased weight and survival, lower bacterial load, and attenuated intestinal pathology compared with wild-type mice. These protective effects involved modulation of NF-κB activation, TNF production, and NO synthesis in mucosal tissue and macrophages, as well as systemic dysregulation of IL-17 and IFN-γ responses. In vivo neutralization of these proinflammatory cytokines impaired bacterial clearance and eliminated host protection conferred by Gal-1 deficiency. Finally, supplementation of recombinant Gal-1 in mice lacking Gal-1 or treatment of wild-type mice with a neutralizing anti-Gal-1 mAb confirmed the immune inhibitory role of this endogenous lectin during infection. Thus, targeting Gal-1-glycan interactions may contribute to reinforce antibacterial responses by reprogramming innate and adaptive immune mechanisms.
[Mh] Termos MeSH primário: Galectina 1/metabolismo
Interações Hospedeiro-Patógeno
Yersiniose/imunologia
Yersinia enterocolitica/imunologia
[Mh] Termos MeSH secundário: Animais
Carga Bacteriana
Proteínas da Membrana Bacteriana Externa/genética
Proteínas de Bactérias/genética
Galectina 1/antagonistas & inibidores
Galectina 1/genética
Galectina 1/imunologia
Interferon gama/sangue
Interferon gama/imunologia
Interleucina-17/sangue
Interleucina-17/imunologia
Intestinos/imunologia
Intestinos/microbiologia
Intestinos/patologia
Camundongos
NF-kappa B/metabolismo
Óxido Nítrico/biossíntese
Nódulos Linfáticos Agregados/imunologia
Nódulos Linfáticos Agregados/microbiologia
Nódulos Linfáticos Agregados/patologia
Proteínas Tirosina Fosfatases/deficiência
Proteínas Tirosina Fosfatases/genética
Baço/imunologia
Baço/microbiologia
Fator de Necrose Tumoral alfa/biossíntese
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Outer Membrane Proteins); 0 (Bacterial Proteins); 0 (Galectin 1); 0 (Interleukin-17); 0 (NF-kappa B); 0 (Tumor Necrosis Factor-alpha); 0 (YopP protein, Yersinia); 31C4KY9ESH (Nitric Oxide); 82115-62-6 (Interferon-gamma); EC 3.1.3.48 (Protein Tyrosine Phosphatases); EC 3.1.3.48 (yopH protein, Yersinia)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1700579


  4 / 3245 MEDLINE  
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[PMID]:28711901
[Au] Autor:Sydorchuk AS; Holyar OI; Randiuk YO; Sorokhan VD; Sydorchuk LI; Bohachyk NA; Venglovska YV; Sokol AM
[Ad] Endereço:Higher State Educational Establishment Of Ukraine "Bukovinian State Medical University", Chernivtsi, Ukraine.
[Ti] Título:Secondary focal form of yersinia enterocolitica infection with prolonged polyarthritis in young caucasian male: a case report.
[So] Source:Wiad Lek;70(3 pt 1):520-522, 2017.
[Is] ISSN:0043-5147
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Current issue deals with an interesting clinical case of a rare infectious disease in a Caucasian young male patient, caused by Yersinia enterocоlitica. Infection proceeded in the development of secondary focal form, which was accompanied by prolonged polyarthritis. We described a clinical case of secondary focal form with prolonged polyarthritis caused by Y. enterocolitica O:3 serogroup in young patient with the purpose of focusing on the early clinical and laboratory diagnosistics of Yersiniosis that would minimize the role of medical errors in diagnostics made by general practitioners. This case deserves the attention of internal medicine specialists, physicians of the specialty ≪general practitioners≫, rheumatologists, infectious disease specialists taking into consideration the clinics and immunopathogenesis, as well as a high evidence of a prolonged clinical course and chronicity of this disease. It has accented on the feasibility of early serological diagnostics and etiotropic antibiotic therapy of the disease.
[Mh] Termos MeSH primário: Artrite/etiologia
Yersiniose/complicações
Yersinia enterocolitica
[Mh] Termos MeSH secundário: Artrite/microbiologia
Artrite/patologia
Seres Humanos
Masculino
Yersiniose/diagnóstico
Yersiniose/patologia
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170717
[St] Status:MEDLINE


  5 / 3245 MEDLINE  
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[PMID]:28627490
[Au] Autor:Duman M; Altun S; Cengiz M; Saticioglu IB; Buyukekiz AG; Sahinturk P
[Ad] Endereço:Uludag University, Faculty of Veterinary Medicine, Aquatic Animal Disease Department, 16059 Bursa, Turkey.
[Ti] Título:Genotyping and antimicrobial resistance genes of Yersinia ruckeri isolates from rainbow trout farms.
[So] Source:Dis Aquat Organ;125(1):31-44, 2017 06 19.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In this study, we compared 142 Yersinia ruckeri isolates collected between 2013 and 2016 from 6 different regions in Turkey. A total of 18 different genogroups were found, though most of the isolates clustered into the same genogroup as serotype O1. As immunization of fish with inactivated Y. ruckeri by injection, immersion, or feeding provide minimal protection against Y. ruckeri infection in Turkey, many fish producers use antimicrobials unrestrictedly, resulting in antimicrobial resistance in aquatic pathogens. Accordingly, we investigated resistance to the antimicrobials most commonly used to treat yersiniosis. More than 80% of the Y. ruckeri isolates were susceptible to sulfamethoxazole-trimethoprim (SXT), florfenicol (FFC), and tetracycline, whereas none were susceptible to sulfamethoxazole. The most commonly used antimicrobials (SXT and FFC) can be effectively administered because the resistance levels to these drugs are the lowest among those reported for agents used to control enteric red mouth disease (12.6 and 14.7%, respectively). In conclusion, to the best of our knowledge, this study is the first characterization of the antimicrobial resistance genes floR, sulI, tetC, tetD, and tetE in Y. ruckeri isolates from aquaculture. Additionally, we detected the sulII gene but not the tetA, tetB, tetM, tetS, or sulIII genes.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Farmacorresistência Bacteriana
Doenças dos Peixes/microbiologia
Genótipo
Yersiniose/veterinária
Yersinia ruckeri/genética
[Mh] Termos MeSH secundário: Animais
Doenças dos Peixes/epidemiologia
Testes de Sensibilidade Microbiana
Oncorhynchus mykiss
Variantes Farmacogenômicos
Turquia/epidemiologia
Yersiniose/epidemiologia
Yersiniose/microbiologia
Yersinia ruckeri/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.3354/dao03132


  6 / 3245 MEDLINE  
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[PMID]:28578732
[Au] Autor:Marimon JM; Figueroa R; Idigoras P; Gomariz M; Alkorta M; Cilla G; Pérez-Trallero E
[Ad] Endereço:Microbiology Department,Hospital Universitario Donostia-IIS-Biodonostia,San Sebastián,Spain.
[Ti] Título:Thirty years of human infections caused by Yersinia enterocolitica in northern Spain: 1985-2014.
[So] Source:Epidemiol Infect;145(11):2197-2203, 2017 08.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Yersinia enterocolitica infection is a zoonosis with worldwide distribution, gastroenteritis being by far the most common clinical manifestation of human infection. In Gipuzkoa, northern Spain, human Y. enterocolitica infections increased from the mid-1980s to the beginning of the 21st century (from 7·9 to 23·2 annual episodes per 100 000 population) to decrease to 7·2 annual episodes per 100 000 population in the last years of the study. The hospital admission rate due to yersiniosis during the last 15 years of the study was 7·3%. More than 99% of isolates were serotype O:3. Infection affected mainly children under 5 years of age (average rate: 140 episodes per 100 000 population). The incidence in adults was low but hospitalisation increased with age, exceeding 50% in people over 64 years old.
[Mh] Termos MeSH primário: Yersiniose/epidemiologia
Yersinia enterocolitica/fisiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Criança
Pré-Escolar
Feminino
Gastroenterite/tratamento farmacológico
Gastroenterite/epidemiologia
Gastroenterite/microbiologia
Seres Humanos
Incidência
Lactente
Recém-Nascido
Masculino
Meia-Idade
Espanha/epidemiologia
Yersiniose/tratamento farmacológico
Yersiniose/microbiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.1017/S095026881700108X


  7 / 3245 MEDLINE  
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[PMID]:28502944
[Au] Autor:Honda K; Iwanaga N; Izumi Y; Tsuji Y; Kawahara C; Michitsuji T; Higashi S; Kawakami A; Migita K
[Ad] Endereço:Department of Rheumatology and General Internal Medicine, Nagasaki Medical Center, Japan.
[Ti] Título:Reactive Arthritis Caused by Yersinia enterocolitica Enteritis.
[So] Source:Intern Med;56(10):1239-1242, 2017.
[Is] ISSN:1349-7235
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:We report a case of reactive arthritis (ReA) triggered by Yersinia enterocolitica enteritis. A 24-year-old Japanese man developed polyarthritis in the lower limbs. Two weeks prior to these symptoms, he noted diarrhea, right lower abdominal pain and a fever. Y. enterocolitica was not isolated from a stool culture; however, he was diagnosed with ReA based on the colonoscopic findings of a high anti-Y. enterocolitica antibody titer and HLA-B27 antigen positivity. Following treatment with methotrexate and steroids, his arthritis improved. This is the first reported Japanese case of ReA in the English literature after a gastrointestinal infection caused by Y. enterocolitica.
[Mh] Termos MeSH primário: Antirreumáticos/uso terapêutico
Artrite Reativa/tratamento farmacológico
Artrite Reativa/etiologia
Metotrexato/uso terapêutico
Esteroides/uso terapêutico
Yersiniose/complicações
[Mh] Termos MeSH secundário: Adulto
Grupo com Ancestrais do Continente Asiático
Gastroenteropatias/complicações
Gastroenteropatias/tratamento farmacológico
Seres Humanos
Masculino
Resultado do Tratamento
Yersinia enterocolitica/efeitos dos fármacos
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antirheumatic Agents); 0 (Steroids); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.2169/internalmedicine.56.7888


  8 / 3245 MEDLINE  
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[PMID]:28403184
[Au] Autor:Rouffaer LO; Baert K; Van den Abeele AM; Cox I; Vanantwerpen G; De Zutter L; Strubbe D; Vranckx K; Lens L; Haesebrouck F; Delmée M; Pasmans F; Martel A
[Ad] Endereço:Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
[Ti] Título:Low prevalence of human enteropathogenic Yersinia spp. in brown rats (Rattus norvegicus) in Flanders.
[So] Source:PLoS One;12(4):e0175648, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Brown rats (Rattus norvegicus) have been identified as potential carriers of Yersinia enterocolitica and Y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in Europe. Enteropathogenic Yersinia spp. are most often isolated from rats during yersiniosis cases in animals and humans, and from rats inhabiting farms and slaughterhouses. Information is however lacking regarding the extent to which rats act as carriers of these Yersinia spp.. In 2013, 1088 brown rats across Flanders, Belgium, were tested for the presence of Yersinia species by isolation method. Identification was performed using MALDI-TOF MS, PCR on chromosomal- and plasmid-borne virulence genes, biotyping and serotyping. Yersinia spp. were isolated from 38.4% of the rats. Of these, 53.4% were designated Y. enterocolitica, 0.7% Y. pseudotuberculosis and 49.0% other Yersinia species. Two Y. enterocolitica possessing the virF-, ail- and ystA-gene were isolated. Additionally, the ystB-gene was identified in 94.1% of the other Y. enterocolitica isolates, suggestive for biotype 1A. Three of these latter isolates simultaneously possessed the ail-virulence gene. Significantly more Y. enterocolitica were isolated during winter and spring compared to summer. Based on our findings we can conclude that brown rats are frequent carriers for various Yersinia spp., including Y. pseudotuberculosis and (human pathogenic) Y. enterocolitica which are more often isolated during winter and spring.
[Mh] Termos MeSH primário: Doenças dos Roedores/microbiologia
Yersiniose/veterinária
Yersinia enterocolitica/genética
Yersinia pseudotuberculosis/genética
[Mh] Termos MeSH secundário: Matadouros
Animais
Bélgica/epidemiologia
Reservatórios de Doenças
Genes Bacterianos
Técnicas de Diagnóstico Molecular
Prevalência
Ratos
Doenças dos Roedores/epidemiologia
Fatores de Virulência/genética
Yersiniose/epidemiologia
Yersiniose/microbiologia
Yersinia enterocolitica/isolamento & purificação
Yersinia pseudotuberculosis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Virulence Factors)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175648


  9 / 3245 MEDLINE  
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[PMID]:28378975
[Au] Autor:Bancerz-Kisiel A; Szczerba-Turek A; Platt-Samoraj A; Michalczyk M; Szweda W
[Ad] Endereço:Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury, Olsztyn, Poland.
[Ti] Título:A study of single nucleotide polymorphism in the ystB gene of Yersinia enterocolitica strains isolated from various wild animal species.
[So] Source:Ann Agric Environ Med;24(1):56-61, 2017 Mar 01.
[Is] ISSN:1898-2263
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION AND OBJECTIVE: Y. enterocolitica is the causative agent of yersiniosis. The objective of the article was a study of single nucleotide polymorphism in the ystB gene of Y. enterocolitica strains isolated from various wild animal species. MATERIALS AND METHOD: High-resolution melting (HRM) analysis was applied to identify single nucleotide polymorphism (SNP) of ystB gene fragments of 88 Y. enterocolitica biotype 1A strains isolated from wild boar, roe deer, red deer and wild ducks. RESULTS: HRM analysis revealed 14 different melting profiles - 4 of them were defined as regular genotypes (G1, G2, G3, G4), whereas 10 as variations. 24 of the examined Y. enterocolitica strains were classified as G1, 18 strains as a G2, 21 strains as a G3, and 15 strains as a G4. Nucleotide sequences classified as G1 revealed 100% similarity with the Y. enterocolitica D88145.1 sequence (NCBI). Analysis of G2 revealed one point mutation - transition T111A. One mutation was also found in G3, but SNP was placed in a different gene region - transition G193A. Two SNPs - transitions G92C and T111A - were identified in G4. Direct sequencing of 10 variations revealed 5 new variants of the ystB nucleotide sequence: V1 - transition G129A (3 strains); V2 - transitions T111A and G193A (2 strains); V3 - transitions C118T and G193A (1 strain); V4 - transitions C141A and G193A (2 strains); and V5 characterized by 19 SNPs: G83A, T93A, A109G, G114T, C116T, A123G, T134C, T142G, T144C, A150C, G162A, T165G, T170G, T174A, T177G, G178A, A179G, A184G and G193A (2 strains). The predominant genotype in isolates from wild ducks was G1; in red deer G2; in wild boar G3; in roe deer G1 and G4. CONCLUSIONS: The proposed HRM method could be used to analyze Y. enterocolitica biotype 1A strains isolated from different sources, including humans.
[Mh] Termos MeSH primário: Polimorfismo de Nucleotídeo Único
Yersiniose/veterinária
Yersinia enterocolitica/genética
[Mh] Termos MeSH secundário: Animais
Animais Selvagens
Cervos
Patos
Sus scrofa
Yersiniose/genética
Yersiniose/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.5604/12321966.1230737


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[PMID]:28332937
[Au] Autor:Joutsen S; Laukkanen-Ninios R; Henttonen H; Niemimaa J; Voutilainen L; Kallio ER; Helle H; Korkeala H; Fredriksson-Ahomaa M
[Ad] Endereço:1 Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki , Helsinki, Finland .
[Ti] Título:Yersinia spp. in Wild Rodents and Shrews in Finland.
[So] Source:Vector Borne Zoonotic Dis;17(5):303-311, 2017 May.
[Is] ISSN:1557-7759
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Yersinia enterocolitica and Yersinia pseudotuberculosis are important zoonotic bacteria causing human enteric yersiniosis commonly reported in Europe. All Y. pseudotuberculosis strains are considered pathogenic, while Y. enterocolitica include both pathogenic and nonpathogenic strains which can be divided into six biotypes (1A, 1B, and 2-5) and about 30 serotypes. The most common types causing yersiniosis in Europe are Y. enterocolitica bioserotypes 4/O:3 and 2/O:9. Strains belonging to biotype 1A are considered as nonpathogenic because they are missing important virulence genes like the attachment-invasion-locus (ail) gene in the chromosome and the virulence plasmid. The role of wild small mammals as a reservoir of enteropathogenic Yersinia spp. is still obscure. In this study, the presence of Yersinia spp. was examined from 1840 wild small mammals, including voles, mice, and shrews, trapped in Finland during a 7-year period. We isolated seven Yersinia species. Y. enterocolitica was the most common species, isolated from 8% of the animals; while most of these isolates represented nonpathogenic biotype 1A, human pathogenic bioserotype 2/O:9 was also isolated from a field vole. Y. pseudotuberculosis of bioserotype 1/O:2 was isolated from two shrews. The ail gene, which is typically only found in the isolates of biotypes 1B and 2-5 associated with yersiniosis, was frequently (23%) detected in the nonpathogenic isolates of biotype 1A and sporadically (6%) in Yersinia kristensenii isolates. Our results suggest that wild small mammals, especially voles, may serve as carriers for ail-positive Y. enterocolitica 1A and Y. kristensenii. We also demonstrate that voles and shrews sporadically excrete pYV-positive Y. enterocolitica 2/O:9 and Y. pseudotuberculosis 1/O:2, respectively, in their feces and, thus, can serve as a contamination source for vegetables by contaminating the soil.
[Mh] Termos MeSH primário: Animais Selvagens
Doenças dos Roedores/microbiologia
Roedores
Musaranhos/microbiologia
Yersiniose/veterinária
Yersinia/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Finlândia/epidemiologia
Doenças dos Roedores/epidemiologia
Especificidade da Espécie
Yersinia/classificação
Yersiniose/epidemiologia
Yersiniose/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1089/vbz.2016.2025



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