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[PMID]:28284596
[Au] Autor:Pomorska-Mól M; Dors A; Kwit K; Kowalczyk A; Stasiak E; Pejsak Z
[Ad] Endereço:National Veterinary Research Institute, Department of Swine Diseases, Pulawy, Poland. Electronic address: mpomorska@piwet.pulawy.pl.
[Ti] Título:Kinetics of single and dual infection of pigs with swine influenza virus and Actinobacillus pleuropneumoniae.
[So] Source:Vet Microbiol;201:113-120, 2017 Mar.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Porcine respiratory disease complex (PRDC) is a common problem in modern pork production worldwide. Pathogens that are amongst other pathogens frequently involved in PRDC etiology are swine influenza virus (SIV) and A. pleuropneumoniae. The effect of dual infection with mentioned pathogens has not been investigated to date. The aim of the present study was to evaluate the kinetics of single and dual infection of pigs with SIV and A. pleuropneumoniae with regard to clinical course, pathogens shedding, lung lesions and early immune response. The most severe symptoms were observed in co-inoculated piglets. The AUC value for SIV shedding was lower in pigs single inoculated with SIV as compared to co-inoculated animals. In contrast, no significant differences were found between A. pleuropneumoniae shedding in single or dual inoculated pigs. Three out of 5 co-inoculated piglets euthanized at 10 dpi were positive against serotype 2 A. pleuropneumonie. All piglets inoculated with SIV developed specific HI antibodies at 10 dpi. In pigs dual inoculated the specific humoral response against SIV was observed earlier, at 7 dpi. The SIV-like lung lesions were more severe in co-inoculated pigs. In the groups inoculated with A. pleuropneumoniae (single or dual) the acute phase protein response was generally stronger than in SIV-single infected group. Co-infection with SIV and A. pleuropneumoniae potentiated the severity of lung lesions caused by SIV and enhanced virus replication in the lung and nasal SIV shedding. Enhanced SIV replication contributed to a more severe clinical course of the disease as well as earlier and higher magnitude immune response (acute phase proteins, HI antibodies) compared to single inoculated pigs.
[Mh] Termos MeSH primário: Infecções por Actinobacillus/veterinária
Actinobacillus pleuropneumoniae/imunologia
Vírus da Influenza A/imunologia
Infecções por Orthomyxoviridae/veterinária
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Infecções por Actinobacillus/microbiologia
Animais
Coinfecção/veterinária
Imunidade Humoral
Cinética
Pulmão/microbiologia
Pulmão/patologia
Pulmão/virologia
Infecções por Orthomyxoviridae/virologia
Suínos
Doenças dos Suínos/virologia
Eliminação de Partículas Virais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


  2 / 1208 MEDLINE  
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[PMID]:28245826
[Au] Autor:Sassu EL; Frömbling J; Duvigneau JC; Miller I; Müllebner A; Gutiérrez AM; Grunert T; Patzl M; Saalmüller A; von Altrock A; Menzel A; Ganter M; Spergser J; Hewicker-Trautwein M; Verspohl J; Ehling-Schulz M; Hennig-Pauka I
[Ad] Endereço:University Clinic for Swine, Department of Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Vienna, Austria.
[Ti] Título:Host-pathogen interplay at primary infection sites in pigs challenged with Actinobacillus pleuropneumoniae.
[So] Source:BMC Vet Res;13(1):64, 2017 Feb 28.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Actinobacillus (A.) pleuropneumoniae is the causative agent of porcine pleuropneumonia and causes significant losses in the pig industry worldwide. Early host immune response is crucial for further progression of the disease. A. pleuropneumoniae is either rapidly eliminated by the immune system or switches to a long-term persistent form. To gain insight into the host-pathogen interaction during the early stages of infection, pigs were inoculated intratracheally with A. pleuropneumoniae serotype 2 and humanely euthanized eight hours after infection. Gene expression studies of inflammatory cytokines and the acute phase proteins haptoglobin, serum amyloid A and C-reactive protein were carried out by RT-qPCR from the lung, liver, tonsils and salivary gland. In addition, the concentration of cytokines and acute phase proteins were measured by quantitative immunoassays in bronchoalveolar lavage fluid, serum and saliva. In parallel to the analyses of host response, the impact of the host on the bacterial pathogen was assessed on a metabolic level. For the latter, Fourier-Transform Infrared (FTIR-) spectroscopy was employed. RESULTS: Significant cytokine and acute phase protein gene expression was detected in the lung and the salivary gland however this was not observed in the tonsils. In parallel to the analyses of host response, the impact of the host on the bacterial pathogen was assessed on a metabolic level. For the latter investigations, Fourier-Transform Infrared (FTIR-) spectroscopy was employed. The bacteria isolated from the upper and lower respiratory tract showed distinct IR spectral patterns reflecting the organ-specific acute phase response of the host. CONCLUSIONS: In summary, this study implies a metabolic adaptation of A. pleuropneumoniae to the porcine upper respiratory tract already during early infection, which might indicate a first step towards the persistence of A. pleuropneumoniae. Not only in lung, but also in the salivary gland an increased inflammatory gene expression was detectable during the acute stage of infection.
[Mh] Termos MeSH primário: Infecções por Actinobacillus/veterinária
Actinobacillus pleuropneumoniae
Pleuropneumonia/veterinária
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Infecções por Actinobacillus/imunologia
Infecções por Actinobacillus/metabolismo
Infecções por Actinobacillus/microbiologia
Actinobacillus pleuropneumoniae/imunologia
Actinobacillus pleuropneumoniae/isolamento & purificação
Actinobacillus pleuropneumoniae/metabolismo
Animais
Citocinas/metabolismo
Pleuropneumonia/imunologia
Pleuropneumonia/metabolismo
Pleuropneumonia/microbiologia
Suínos
Doenças dos Suínos/imunologia
Doenças dos Suínos/metabolismo
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170302
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-017-0979-6


  3 / 1208 MEDLINE  
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[PMID]:28166835
[Au] Autor:Sassu EL; Ladinig A; Talker SC; Stadler M; Knecht C; Stein H; Frömbling J; Richter B; Spergser J; Ehling-Schulz M; Graage R; Hennig-Pauka I; Gerner W
[Ad] Endereço:University Clinic for Swine, Department of Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria.
[Ti] Título:Frequency of Th17 cells correlates with the presence of lung lesions in pigs chronically infected with Actinobacillus pleuropneumoniae.
[So] Source:Vet Res;48(1):4, 2017 Feb 06.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Porcine contagious pleuropneumonia caused by Actinobacillus pleuropneumoniae (APP) remains one of the major causes of poor growth performance and respiratory disease in pig herds. While the role of antibodies against APP has been intensely studied, the porcine T cell response remains poorly characterized. To address this, pigs were intranasally infected with APP serotype 2 and euthanized during the acute phase [6-10 days post-infection (dpi)] or the chronic phase of APP infection (27-31 dpi). Lymphocytes isolated from blood, tonsils, lung tissue and tracheobronchial lymph nodes were analyzed by intracellular cytokine staining (ICS) for IL-17A, IL-10 and TNF-α production after in vitro stimulation with crude capsular extract (CCE) of the APP inoculation strain. This was combined with cell surface staining for the expression of CD4, CD8α and TCR-γδ. Clinical records, microbiological investigations and pathological findings confirmed the induction of a subclinical APP infection. ICS-assays revealed the presence of APP-CCE specific CD4 CD8α IL-17A-producing T cells in blood and lung tissue in most infected animals during the acute and chronic phase of infection and a minor fraction of these cells co-produced TNF-α. APP-CCE specific IL-17A-producing γδ T cells could not be found and APP-CCE specific IL-10-producing CD4 T cells were present in various organs but only in a few infected animals. The frequency of identified putative Th17 cells (CD4 CD8α IL-17A ) in lung and blood correlated positively with lung lesion scores and APP-specific antibody titers during the chronic phase. These results suggest a potential role of Th17 cells in the immune pathogenesis of APP infection.
[Mh] Termos MeSH primário: Infecções por Actinobacillus/veterinária
Actinobacillus pleuropneumoniae
Pulmão/patologia
Pleuropneumonia/veterinária
Doenças dos Suínos/microbiologia
Células Th17/patologia
[Mh] Termos MeSH secundário: Infecções por Actinobacillus/imunologia
Infecções por Actinobacillus/microbiologia
Infecções por Actinobacillus/patologia
Actinobacillus pleuropneumoniae/imunologia
Animais
Doença Crônica
Pulmão/imunologia
Pulmão/microbiologia
Linfonodos/patologia
Masculino
Pleuropneumonia/imunologia
Pleuropneumonia/microbiologia
Pleuropneumonia/patologia
Suínos
Doenças dos Suínos/imunologia
Doenças dos Suínos/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1186/s13567-017-0411-z


  4 / 1208 MEDLINE  
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[PMID]:28113129
[Au] Autor:Dorey L; Hobson S; Lees P
[Ad] Endereço:The Royal Veterinary College, Department of Comparative Biological Sciences, Hawkshead Campus, Hatfield, Herts AL9 7TA, United Kingdom. Electronic address: ldorey@rvc.ac.uk.
[Ti] Título:Factors influencing the potency of marbofloxacin for pig pneumonia pathogens Actinobacillus pleuropneumoniae and Pasteurella multocida.
[So] Source:Res Vet Sci;111:93-98, 2017 Apr.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:For the pig respiratory tract pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida, Minimum Inhibitory Concentration (MIC) of marbofloxacin was determined in recommended broths and pig serum at three inoculum strengths. MICs in both growth matrices increased progressively from low, through medium to high starting inoculum counts, 10 , 10 and 10 CFU/mL, respectively. P. multocida MIC ratios for high:low inocula were 14:4:1 for broth and 28.2:1 for serum. Corresponding MIC ratios for A. pleuropneumoniae were lower, 4.1:1 (broth) and 9.2:1 (serum). MIC high:low ratios were therefore both growth matrix and bacterial species dependent. The effect of alterations to the chemical composition of broths and serum on MIC were also investigated. Neither adjusting broth or serum pH in six increments over the range 7.0 to 8.0 nor increasing calcium and magnesium concentrations of broth in seven incremental steps significantly affected MICs for either organism. In time-kill studies, the killing action of marbofloxacin had the characteristics of concentration dependency against both organisms in both growth matrices. It is concluded that MIC and time-kill data for marbofloxacin, generated in serum, might be preferable to broth data, for predicting dosages of marbofloxacin for clinical use.
[Mh] Termos MeSH primário: Infecções por Actinobacillus/veterinária
Actinobacillus pleuropneumoniae/efeitos dos fármacos
Antibacterianos/farmacologia
Fluoroquinolonas/farmacologia
Infecções por Pasteurella/veterinária
Pasteurella multocida/efeitos dos fármacos
Doenças dos Suínos/prevenção & controle
[Mh] Termos MeSH secundário: Infecções por Actinobacillus/microbiologia
Infecções por Actinobacillus/prevenção & controle
Animais
Concentração de Íons de Hidrogênio
Testes de Sensibilidade Microbiana
Infecções por Pasteurella/microbiologia
Infecções por Pasteurella/prevenção & controle
Pneumonia/microbiologia
Pneumonia/prevenção & controle
Pneumonia/veterinária
Suínos
Doenças dos Suínos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Fluoroquinolones); 8X09WU898T (marbofloxacin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE


  5 / 1208 MEDLINE  
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[PMID]:28061786
[Au] Autor:Xie F; Li G; Zhou L; Zhang Y; Cui N; Liu S; Wang C
[Ad] Endereço:State Key Laboratory of Veterinary Biotechnology, Division of Bacterial Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, People's Republic of China.
[Ti] Título:Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8∆clpP/apxIIC confers protection against homologous or heterologous strain challenge.
[So] Source:BMC Vet Res;13(1):14, 2017 Jan 06.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, which leads to large economic losses to the swine industry worldwide. In this study, S-8â–³clpPâ–³apxIIC, a double-deletion mutant of A. pleuropneumoniae was constructed, and its safety and protective efficacy were evaluated in pigs. RESULTS: The S-8â–³clpPâ–³apxIIC mutant exhibited attenuated virulence in a murine (BALB/c) model, and caused no detrimental effects on pigs even at a dose of up to 1.0 × 10 CFU. Furthermore, the S-8â–³clpPâ–³apxIIC mutant was able to induce a strong immune response in pigs, which included high levels of IgG1 and IgG2, stimulated gamma interferon (IFN-γ), interleukin 12 (IL-12), and interleukin 4 (IL-4) production, and conferred effective protection against the lethal challenge with A. pleuropneumoniae serovars 7 or 5a. The pigs in the S-8â–³clpPâ–³apxIIC immunized groups have no lesions and reduced bacterial loads in the lung tissue after challenge. CONCLUSIONS: The data obtained in this study suggest that the S-8â–³clpPâ–³apxIIC mutant can serve as a highly immunogenic and potential live attenuated vaccine candidate against A. pleuropneumoniae infection.
[Mh] Termos MeSH primário: Infecções por Actinobacillus/veterinária
Actinobacillus pleuropneumoniae/genética
Actinobacillus pleuropneumoniae/imunologia
Vacinas Bacterianas/imunologia
Doenças dos Suínos/prevenção & controle
[Mh] Termos MeSH secundário: Infecções por Actinobacillus/microbiologia
Infecções por Actinobacillus/prevenção & controle
Actinobacillus pleuropneumoniae/metabolismo
Actinobacillus pleuropneumoniae/patogenicidade
Animais
Deleção de Genes
Camundongos
Camundongos Endogâmicos BALB C
Suínos
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Bacterial Vaccines)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-016-0928-9


  6 / 1208 MEDLINE  
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[PMID]:28053219
[Au] Autor:Bossé JT; Li Y; Sárközi R; Gottschalk M; Angen Ø; Nedbalcova K; Rycroft AN; Fodor L; Langford PR
[Ad] Endereço:Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom j.bosse@imperial.ac.uk p.langford@imperial.ac.uk.
[Ti] Título:A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay.
[So] Source:J Clin Microbiol;55(3):902-907, 2017 Mar.
[Is] ISSN:1098-660X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:causes pleuropneumonia, an economically significant lung disease of pigs. Recently, isolates of that were serologically distinct from the previously characterized 15 serovars were described, and a proposal was put forward that they comprised a new serovar, serovar 16. Here we used whole-genome sequencing of the proposed serovar 16 reference strain A-85/14 to confirm the presence of a unique capsular polysaccharide biosynthetic locus. For molecular diagnostics, primers were designed from the capsule locus of strain A-85/14, and a PCR was formulated that differentiated serovar 16 isolates from all 15 known serovars and other common respiratory pathogenic/commensal bacteria of pigs. Analysis of the capsule locus of strain A-85/14 combined with the previous serological data show the existence of a sixteenth serovar-designated serovar 16-of .
[Mh] Termos MeSH primário: Infecções por Actinobacillus/veterinária
Actinobacillus pleuropneumoniae/classificação
Cápsulas Bacterianas/genética
Loci Gênicos
Reação em Cadeia da Polimerase/métodos
Sorogrupo
Doenças dos Suínos/diagnóstico
[Mh] Termos MeSH secundário: Infecções por Actinobacillus/diagnóstico
Actinobacillus pleuropneumoniae/genética
Animais
Primers do DNA/genética
DNA Bacteriano/química
DNA Bacteriano/genética
Genoma Bacteriano
Técnicas de Diagnóstico Molecular/métodos
Pleuropneumonia/microbiologia
Pleuropneumonia/veterinária
Análise de Sequência de DNA
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Bacterial)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE
[do] DOI:10.1128/JCM.02166-16


  7 / 1208 MEDLINE  
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[PMID]:27940254
[Au] Autor:Qin W; Wang L; Zhai R; Ma Q; Liu J; Bao C; Sun D; Zhang H; Sun C; Feng X; Gu J; Du C; Han W; Langford PR; Lei L
[Ad] Endereço:College of Veterinary Medicine, Jilin University, Changchun, PR China.
[Ti] Título:Apa2H1, the first head domain of Apa2 trimeric autotransporter adhesin, activates mouse bone marrow-derived dendritic cells and immunization with Apa2H1 protects against Actinobacillus pleuropneumoniae infection.
[So] Source:Mol Immunol;81:108-117, 2017 Jan.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Actinobacillus pleuropneumoniae is the causative pathogen of porcine pleuropneumonia, which results in large economic losses in the pig industry worldwide. There are, however, no effective subunit vaccines are available in the market owing to the various serotypes and the absence of cross-protection against this pathogen. Therefore, the selection of protective components is of great significance for vaccine development. We previously showed that trimeric autotransporter adhesins are important virulence factors of A. pleuropneumoniae. To determine the potential role in vaccine development of the functional head domain (Apa2H1) of Apa2, a trimeric autotransporter adhesin found in A. pleuropneumoniae, we obtained nature-like trimeric Apa2H1 using a prokaryotic expression system and co-culture of Apa2H1 with bone marrow derived dendritic cells (BMDCs) in vitro resulted in maturation of BMDCs, characterised by the up-regulation of CD83, MHC-II, CCR7, ICAM-I and the increased expression of factors related to B lymphoid cells stimulation, such as proliferation-inducing ligand (APRIL), B lymphocyte stimulator (BLyS) and B cell activating factor (BAFF). The in vivo results showed that vaccination with Apa2H1 resulted in the robust production of antigen-specific antibodies, modestly induced mixed Th1 and Th2 immunity, impaired bacterial colonization and dissemination, and improved mouse survival rates. This study is the first to show that Apa2H1 is antigenic and can be used as a component of a subunit vaccine against A. pleuropneumoniae infection, providing valuable reference material for the development of an effective vaccine against A. pleuropneumoniae.
[Mh] Termos MeSH primário: Infecções por Actinobacillus/imunologia
Adesinas Bacterianas/imunologia
Vacinas Bacterianas/imunologia
Células da Medula Óssea/imunologia
Células Dendríticas/imunologia
[Mh] Termos MeSH secundário: Actinobacillus pleuropneumoniae/patogenicidade
Animais
Western Blotting
Modelos Animais de Doenças
Feminino
Citometria de Fluxo
Camundongos
Camundongos Endogâmicos BALB C
Domínios Proteicos
Reação em Cadeia da Polimerase em Tempo Real
Vacinação
Vacinas de Subunidades/imunologia
Fatores de Virulência/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adhesins, Bacterial); 0 (Bacterial Vaccines); 0 (Vaccines, Subunit); 0 (Virulence Factors)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161213
[St] Status:MEDLINE


  8 / 1208 MEDLINE  
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[PMID]:27694188
[Au] Autor:Ito H; Ogawa T; Fukamizu D; Morinaga Y; Kusumoto M
[Ad] Endereço:The National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan (Ito, Kusumoto)Fukuoka Chuo Livestock Hygiene Center, Higashi-ku, Fukuoka, Japan (Ogawa, Fukamizu, Morinaga) itohiroy@affrc.go.jp.
[Ti] Título:Nucleotide sequence analysis of a DNA region involved in capsular polysaccharide biosynthesis reveals the molecular basis of the nontypeability of two Actinobacillus pleuropneumoniae isolates.
[So] Source:J Vet Diagn Invest;28(6):632-637, 2016 Nov.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of our study was to reveal the molecular basis of the serologic nontypeability of 2 Actinobacillus pleuropneumoniae field isolates. Nine field strains of A. pleuropneumoniae, the causative agent of porcine pleuropneumonia, were isolated from pigs raised on the same farm and sent to our diagnostic laboratory for serotyping. Seven of the 9 strains were identified as serovar 15 strains by immunodiffusion tests. However, 2 strains, designated FH24-2 and FH24-5, could not be serotyped with antiserum prepared against serovars 1-15. Strain FH24-5 showed positive results in 2 serovar 15-specific PCR tests, whereas strain FH24-2 was only positive in 1 of the 2 PCR tests. The nucleotide sequence analysis of gene clusters involved in capsular polysaccharide biosynthesis of the 2 nontypeable strains revealed that both had been rendered nontypeable by the action of ISApl1, a transposable element of A. pleuropneumoniae belonging to the IS30 family. The results showed that ISApl1 of A. pleuropneumoniae can interfere with both the serologic and molecular typing methods, and that nucleotide sequence analysis across the capsular gene clusters is the best means of determining the cause of serologic nontypeability in A. pleuropneumoniae.
[Mh] Termos MeSH primário: Infecções por Actinobacillus/veterinária
Actinobacillus pleuropneumoniae/genética
Pleuropneumonia/veterinária
Polissacarídeos/biossíntese
Doenças dos Suínos/diagnóstico
[Mh] Termos MeSH secundário: Infecções por Actinobacillus/diagnóstico
Infecções por Actinobacillus/microbiologia
Actinobacillus pleuropneumoniae/metabolismo
Animais
Imunodifusão/veterinária
Pleuropneumonia/diagnóstico
Pleuropneumonia/microbiologia
Reação em Cadeia da Polimerase/veterinária
Análise de Sequência de DNA/veterinária
Sorogrupo
Sorotipagem/veterinária
Suínos
Doenças dos Suínos/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polysaccharides)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161004
[St] Status:MEDLINE


  9 / 1208 MEDLINE  
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[PMID]:27606818
[Au] Autor:van Dixhoorn ID; Reimert I; Middelkoop J; Bolhuis JE; Wisselink HJ; Groot Koerkamp PW; Kemp B; Stockhofe-Zurwieden N
[Ad] Endereço:Livestock Research, Wageningen University and Research Centre, Wageningen, the Netherlands.
[Ti] Título:Enriched Housing Reduces Disease Susceptibility to Co-Infection with Porcine Reproductive and Respiratory Virus (PRRSV) and Actinobacillus pleuropneumoniae (A. pleuropneumoniae) in Young Pigs.
[So] Source:PLoS One;11(9):e0161832, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Until today, anti-microbial drugs have been the therapy of choice to combat bacterial diseases. Resistance against antibiotics is of growing concern in man and animals. Stress, caused by demanding environmental conditions, can reduce immune protection in the host, influencing the onset and outcome of infectious diseases. Therefore psychoneuro-immunological intervention may prove to be a successful approach to diminish the impact of diseases and antibiotics use. This study was designed to investigate the effect of social and environmental enrichment on the impact of disease, referred to as "disease susceptibility", in pigs using a co-infection model of PRRSV and A. pleuropneumoniae. Twenty-eight pigs were raised in four pens under barren conditions and twenty-eight other pigs were raised in four pens under enriched conditions. In the enriched pens a combination of established social and environmental enrichment factors were introduced. Two pens of the barren (BH) and two pens of the enriched housed (EH) pigs were infected with PRRSV followed by A. pleuropneumoniae, the other two pens in each housing treatment served as control groups. We tested if differences in disease susceptibility in terms of pathological and clinical outcome were related to the different housing regimes and if this was reflected in differences in behavioural and immunological states of the animals. Enriched housed pigs showed a faster clearance of viral PRRSV RNA in blood serum (p = 0.014) and histologically 2.8 fold less interstitial pneumonia signs in the lungs (p = 0.014). More barren housed than enriched housed pigs developed lesions in the lungs (OR = 19.2, p = 0.048) and the lesions in the barren housed pigs showed a higher total pathologic tissue damage score (p<0.001) than those in enriched housed pigs. EH pigs showed less stress-related behaviour and differed immunologically and clinically from BH pigs. We conclude that enriched housing management reduces disease susceptibility to co-infection of PRRSV and A. pleuropneumoniae in pigs. Enrichment positively influences behavioural state, immunological response and clinical outcome in pigs.
[Mh] Termos MeSH primário: Actinobacillus pleuropneumoniae/fisiologia
Coinfecção/microbiologia
Coinfecção/virologia
Abrigo para Animais
Vírus da Síndrome Respiratória e Reprodutiva Suína/fisiologia
Doenças dos Suínos/microbiologia
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Infecções por Actinobacillus/sangue
Infecções por Actinobacillus/complicações
Infecções por Actinobacillus/virologia
Animais
Anticorpos/metabolismo
Comportamento Animal
Biomarcadores/metabolismo
Temperatura Corporal
Líquido da Lavagem Broncoalveolar/citologia
Coinfecção/sangue
Suscetibilidade a Doenças
Feminino
Citometria de Fluxo
Contagem de Leucócitos
Pulmão/microbiologia
Pulmão/patologia
Pulmão/virologia
Masculino
Fenótipo
Síndrome Respiratória e Reprodutiva Suína/sangue
Síndrome Respiratória e Reprodutiva Suína/virologia
RNA Viral/sangue
Pele/microbiologia
Pele/patologia
Pele/virologia
Sus scrofa
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (Biomarkers); 0 (RNA, Viral)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170804
[Lr] Data última revisão:
170804
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160909
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0161832


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[PMID]:27531715
[Au] Autor:Li Y; Bossé JT; Williamson SM; Maskell DJ; Tucker AW; Wren BW; Rycroft AN; Langford PR; BRADP1T Consortium
[Ad] Endereço:Section of Paediatrics, Imperial College London, St Mary's Campus, London W2 1PG, UK.
[Ti] Título:Actinobacillus pleuropneumoniae serovar 8 predominates in England and Wales.
[So] Source:Vet Rec;179(11):276, 2016 Sep 17.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Infecções por Actinobacillus/veterinária
Actinobacillus pleuropneumoniae/classificação
Actinobacillus pleuropneumoniae/isolamento & purificação
Sorogrupo
Doenças dos Suínos/microbiologia
[Mh] Termos MeSH secundário: Infecções por Actinobacillus/epidemiologia
Infecções por Actinobacillus/microbiologia
Animais
Inglaterra/epidemiologia
Suínos
Doenças dos Suínos/epidemiologia
País de Gales/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160818
[St] Status:MEDLINE
[do] DOI:10.1136/vr.103820



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