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  1 / 6400 MEDLINE  
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[PMID]:28450491
[Ti] Título:Mycobacterium microti infection in a cow in France.
[So] Source:Vet Rec;180(17):429, 2017 04 29.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Doenças dos Bovinos/diagnóstico
Doenças dos Bovinos/virologia
Infecções por Mycobacterium/veterinária
Mycobacterium/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Bovinos
França
Mycobacterium/classificação
Infecções por Mycobacterium/diagnóstico
Infecções por Mycobacterium/virologia
[Pt] Tipo de publicação:CASE REPORTS; LETTER
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180117
[Lr] Data última revisão:
180117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1136/vr.j2041


  2 / 6400 MEDLINE  
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[PMID]:29228045
[Au] Autor:Chao WC; Yen CL; Hsieh CY; Huang YF; Tseng YL; Nigrovic PA; Shieh CC
[Ad] Endereço:Institute of Clinical Medicine, National Cheng Kung University Medical College, Tainan, Taiwan.
[Ti] Título:Mycobacterial infection induces higher interleukin-1ß and dysregulated lung inflammation in mice with defective leukocyte NADPH oxidase.
[So] Source:PLoS One;12(12):e0189453, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Granulomatous inflammation causes severe tissue damage in mycobacterial infection while redox status was reported to be crucial in the granulomatous inflammation. Here, we used a NADPH oxidase 2 (NOX2)-deficient mice (Ncf1-/-) to investigate the role of leukocyte-produced reactive oxygen species (ROS) in mycobacterium-induced granulomatous inflammation. We found poorly controlled mycobacterial proliferation, significant body weight loss, and a high mortality rate after M. marinum infection in Ncf1-/- mice. Moreover, we noticed loose and neutrophilic granulomas and higher levels of interleukin (IL)-1ß and neutrophil chemokines in Ncf1-/- mice when compared with those in wild type mice. The lack of ROS led to reduced production of IL-1ß in macrophages, whereas neutrophil elastase (NE), an abundant product of neutrophils, may potentially exert increased inflammasome-independent protease activity and lead to higher IL-1ß production. Moreover, we showed that the abundant NE and IL-1ß were present in the caseous granulomatous inflammation of human TB infection. Importantly, blocking of IL-1ß with either a specific antibody or a recombinant IL-1 receptor ameliorated the pulmonary inflammation. These findings revealed a novel role of ROS in the early pathogenesis of neutrophilic granulomatous inflammation and suggested a potential role of IL-1 blocking in the treatment of mycobacterial infection in the lung.
[Mh] Termos MeSH primário: Interleucina-1beta/metabolismo
Leucócitos/enzimologia
Infecções por Mycobacterium/metabolismo
NADPH Oxidases/sangue
Pneumonia/metabolismo
[Mh] Termos MeSH secundário: Animais
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Infecções por Mycobacterium/enzimologia
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-1beta); 0 (Reactive Oxygen Species); EC 1.6.3.- (NADPH Oxidases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180104
[Lr] Data última revisão:
180104
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171212
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189453


  3 / 6400 MEDLINE  
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[PMID]:28874233
[Au] Autor:Chew KL; Cheng JWS; Hudaa Osman N; Lin RTP; Teo JWP
[Ad] Endereço:1​National University Hospital, Department of Laboratory Medicine, Division of Microbiology, Singapore 119074, Republic of Singapore.
[Ti] Título:Predominance of clarithromycin-susceptible Mycobacterium massiliense subspecies: Characterization of the Mycobacterium abscessus complex at a tertiary acute care hospital.
[So] Source:J Med Microbiol;66(10):1443-1447, 2017 Oct.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To characterize members of the Mycobacterium abscessus complex, with an emphasis on the correlation between species identification and clarithromycin associated genetic polymorphisms that contribute to inducible and constitutive macrolide resistance. PCR and sequencing analysis was used to elucidate the subspecies, erm(41) genotypes and the presence of rrl mutations. M. abscessus subsp. massiliense was the dominant subspecies (70.2 %), followed by M. abscessus subsp. abscessus (23.8 %) and M. abscessus subsp. bolletii (5.9 %). The majority of M. abscessus and M. bolletii isolates possessed T28 erm(41) sequevar and were inducibly resistant to clarithromycin. All M. massiliense carried the truncated erm(41) and were largely clarithromycin-susceptible (98.3 %). Constitutive resistance involving rrl mutations was rare and seen in only 2 isolates (2.2 %). Subspecies identification was insufficient to predict clarithromycin susceptibility and required the genetic resistance to be determined via sequencing. In our context, rrl mutations were uncommon and may not be an essential test.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Claritromicina/farmacologia
Infecções por Mycobacterium/microbiologia
Mycobacterium/classificação
Mycobacterium/efeitos dos fármacos
Centros de Atenção Terciária
[Mh] Termos MeSH secundário: Seres Humanos
Mycobacterium/genética
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); H1250JIK0A (Clarithromycin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000576


  4 / 6400 MEDLINE  
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[PMID]:28869002
[Au] Autor:Nouioui I; Carro L; Teramoto K; Igual JM; Jando M; Del Carmen Montero-Calasanz M; Sutcliffe I; Sangal V; Goodfellow M; Klenk HP
[Ad] Endereço:1​School of Biology, Ridley Building, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK.
[Ti] Título:Mycobacterium eburneum sp. nov., a non-chromogenic, fast-growing strain isolated from sputum.
[So] Source:Int J Syst Evol Microbiol;67(9):3174-3181, 2017 Sep.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A polyphasic study was undertaken to establish the taxonomic position of a non-chromogenic, rapidly growing Mycobacterium strain that had been isolated from sputum. The strain, CECT 8775T, has chemotaxonomic and cultural properties consistent with its classification in the genus Mycobacterium and was distinguished from the type strains of closely related mycobacterial species, notably from Mycobacterium paraense DSM 46749T, its nearest phylogenetic neighbour, based on 16S rRNA, hsp65 and rpoB gene sequence data. These organisms were also distinguished by a broad range of chemotaxonomic and phenotypic features and by a digital DNA-DNA relatedness value of 22.8 %. Consequently, the strain is considered to represent a novel species of Mycobacterium for which the name Mycobacterium eburneum sp. nov is proposed; the type strain is X82T (CECT 8775T=DSM 44358T).
[Mh] Termos MeSH primário: Mycobacterium/classificação
Filogenia
Escarro/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Genes Bacterianos
Seres Humanos
Mycobacterium/genética
Mycobacterium/isolamento & purificação
Infecções por Mycobacterium/microbiologia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Suíça
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002033


  5 / 6400 MEDLINE  
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[PMID]:28857733
[Au] Autor:Fukano H; Wada S; Kurata O; Katayama K; Fujiwara N; Hoshino Y
[Ad] Endereço:1​Laboratory of Aquatic Medicine, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, 180-8602, Japan.
[Ti] Título:Mycobacterium stephanolepidis sp. nov., a rapidly growing species related to Mycobacterium chelonae, isolated from marine teleost fish, Stephanolepis cirrhifer.
[So] Source:Int J Syst Evol Microbiol;67(8):2811-2817, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A previously undescribed rapidly growing, non-pigmented mycobacterium was identified based on biochemical and nucleic acid analyses, as well as growth characteristics. Seven isolates were cultured from samples collected from five thread-sail filefish (Stephanolepis cirrhifer) and two farmed black scraper (Thamnaconus modestus). Bacterial growth occurred at 15-35 °C on Middlebrook 7H11 agar. The bacteria were positive for catalase activity at 68 °C and urease activity, intermediate for iron uptake, and negative for Tween 80 hydrolysis, nitrate reduction, semi-quantitative catalase activity and arylsulfatase activity at day 3. No growth was observed on Middlebrook 7H11 agar supplemented with picric acid, and very little growth was observed in the presence of 5 % NaCl. α- and α'-mycolates were identified in the cell walls, and a unique profile of the fatty acid methyl esters and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiles of the protein and cell-wall lipids were acquired. Sequence analysis revealed that the seven isolates shared identical sequences for the 16S rRNA, rpoB, hsp65, recA and sodA genes. Phylogenetic analysis of the five gene sequences confirmed that the isolates were unique, but closely related to Mycobacterium chelonae. Antibiotic susceptibility testing revealed the minimum inhibitory concentration (MIC) of clarithromycin against this novel species was <0.25 µg ml , which was lower than that for Mycobacterium salmoniphilum. The hsp65 PCR restriction enzyme analysis pattern differed from those of M. chelonae and M. salmoniphilum. Based on these findings, the name Mycobacterium stephanolepidis sp. nov. is proposed for this novel species, with the type strain being NJB0901 (=JCM 31611 =KCTC 39843 ).
[Mh] Termos MeSH primário: Peixes/microbiologia
Mycobacterium/classificação
Filogenia
[Mh] Termos MeSH secundário: Animais
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Ácidos Graxos/química
Genes Bacterianos
Japão
Mycobacterium/genética
Mycobacterium/isolamento & purificação
Infecções por Mycobacterium/microbiologia
Mycobacterium chelonae
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002028


  6 / 6400 MEDLINE  
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[PMID]:28846409
[Au] Autor:Buchieri MV; Cimino M; Rebollo-Ramirez S; Beauvineau C; Cascioferro A; Favre-Rochex S; Helynck O; Naud-Martin D; Larrouy-Maumus G; Munier-Lehmann H; Gicquel B
[Ad] Endereço:Unité de Génétique Mycobactérienne, Institut Pasteur , 25 Rue du Docteur Roux, 75724 Paris Cedex 15, France.
[Ti] Título:Nitazoxanide Analogs Require Nitroreduction for Antimicrobial Activity in Mycobacterium smegmatis.
[So] Source:J Med Chem;60(17):7425-7433, 2017 Sep 14.
[Is] ISSN:1520-4804
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we aimed to decipher the natural resistance mechanisms of mycobacteria against novel compounds isolated by whole-cell-based high-throughput screening (HTS). We identified active compounds using Mycobacterium aurum. Further analyses were performed to determine the resistance mechanism of M. smegmatis against one hit, 3-bromo-N-(5-nitrothiazol-2-yl)-4-propoxybenzamide (3), which turned out to be an analog of the drug nitazoxanide (1). We found that the repression of the gene nfnB coding for the nitroreductase NfnB was responsible for the natural resistance of M. smegmatis against 3. The overexpression of nfnB resulted in sensitivity of M. smegmatis to 3. This compound must be metabolized into hydroxylamine intermediate for exhibiting antibacterial activity. Thus, we describe, for the first time, the activity of a mycobacterial nitroreductase against 1 analogs, highlighting the differences in the metabolism of nitro compounds among mycobacterial species and emphasizing the potential of nitro drugs as antibacterials in various bacterial species.
[Mh] Termos MeSH primário: Antibacterianos/química
Antibacterianos/farmacologia
Mycobacterium smegmatis/efeitos dos fármacos
Mycobacterium smegmatis/enzimologia
Nitrorredutases/metabolismo
Tiazóis/química
Tiazóis/farmacologia
[Mh] Termos MeSH secundário: Regulação para Baixo
Farmacorresistência Bacteriana
Seres Humanos
Mycobacterium/efeitos dos fármacos
Mycobacterium/enzimologia
Mycobacterium/genética
Infecções por Mycobacterium/tratamento farmacológico
Infecções por Mycobacterium/microbiologia
Infecções por Micobactéria não Tuberculosa/tratamento farmacológico
Infecções por Micobactéria não Tuberculosa/microbiologia
Mycobacterium smegmatis/genética
Nitrorredutases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Thiazoles); EC 1.7.- (Nitroreductases); SOA12P041N (nitazoxanide)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jmedchem.7b00726


  7 / 6400 MEDLINE  
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[PMID]:28837698
[Au] Autor:Dong H; Lv Y; Sreevatsan S; Zhao D; Zhou X
[Ad] Endereço:State Key Laboratory of Agrobiotechnology, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and College of Veterinary Medicine, China Agricultural University, Beijing, China.
[Ti] Título:Differences in pathogenicity of three animal isolates of Mycobacterium species in a mouse model.
[So] Source:PLoS One;12(8):e0183666, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Animal mycobacterioses are among the most important zoonoses worldwide. These are generally caused by either Mycobacterium tuberculosis (MTB), M. bovis (MBO) or M. avium (MAV). To test the hypothesis that different species of pathogenic mycobacteria isolated from varied anatomic locations or animal species differ in virulence and pathogenicity, we performed experiments with three mycobacteria strains (NTSE-3(MTB), NTSE-4(MBO) and NTSE-5 (MAV)) obtained from animal species. Spoligotyping analysis was used to confirm both MTB and MBO strains while the MAV strain was confirmed by 16s rDNA sequencing. BALB/c mice were intranasally infected with the three strains at low and high CFU doses to evaluate variations in pathogenicity. Clinical and pathological parameters were assessed. Infected mice were euthanized at 80 days post-inoculation (dpi). Measures of lung and body weights indicated that the MBO infected group had higher mortality, more weight loss, higher bacterial burden and more severe lesions in lungs than the other two groups. Cytokine profiles showed higher levels of TNF-α for MBO versus MTB, while MAV had the highest amounts of IFN-ß in vitro and in vivo. In vitro levels of other cytokines such as IL-1ß, IL-10, IL-12, IL-17, and IFN-ß showed that Th1 cells had the strongest response in MBO infected mice and that Th2 cells were inhibited. We found that the level of virulence among the three isolates decreased in the following order MBO>MTB>MAV.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Infecções por Mycobacterium/microbiologia
Mycobacterium avium/patogenicidade
Mycobacterium bovis/patogenicidade
Mycobacterium tuberculosis/patogenicidade
[Mh] Termos MeSH secundário: Animais
Peso Corporal
Citocinas/metabolismo
Feminino
Pulmão/microbiologia
Pulmão/patologia
Camundongos
Camundongos Endogâmicos BALB C
Infecções por Mycobacterium/metabolismo
Tamanho do Órgão
Especificidade da Espécie
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183666


  8 / 6400 MEDLINE  
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[PMID]:28809146
[Au] Autor:Davidson RM; DeGroote MA; Marola JL; Buss S; Jones V; McNeil MR; Freifeld AG; Elaine Epperson L; Hasan NA; Jackson M; Iwen PC; Salfinger M; Strong M
[Ad] Endereço:1​Center for Genes, Environment and Health, National Jewish Health, Denver, CO 80206, USA.
[Ti] Título:Mycobacterium talmoniae sp. nov., a slowly growing mycobacterium isolated from human respiratory samples.
[So] Source:Int J Syst Evol Microbiol;67(8):2640-2645, 2017 Aug.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable. The bacteria were acid-fast stain-positive, rod-shaped and exhibited growth after 7-10 days on solid media at temperatures ranging from 25 to 42°C. Colonies were non-pigmented, rough and slightly raised. Analyses of matrix-assisted laser desorption ionization time-of-flight profiles showed no matches against a reference library of 130 mycobacterial species. Full-length 16S rRNA gene sequences were identical for the two isolates, the average nucleotide identity (ANI) between their genomes was 99.7 % and phylogenetic comparisons classified the novel mycobacteria as the basal most species in the slowly growing Mycobacterium clade. Mycobacterium avium is the most closely related species based on rpoB gene sequence similarity (92 %), but the ANI between the genomes was 81.5 %, below the suggested cut-off for differentiating two species (95 %). Mycolic acid profiles were more similar to M. avium than to Mycobacterium simiae or Mycobacterium abscessus. The phenotypic and genomic data support the conclusion that the two related isolates represent a novel Mycobacterium species for which the name Mycobacterium talmoniae sp. nov. is proposed. The type strain is NE-TNMC-100812T (=ATCC BAA-2683T=DSM 46873T).
[Mh] Termos MeSH primário: Mycobacterium/classificação
Filogenia
Sistema Respiratório/microbiologia
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Bacteriano/genética
Genes Bacterianos
Seres Humanos
Mycobacterium/genética
Mycobacterium/isolamento & purificação
Infecções por Mycobacterium/microbiologia
Ácidos Micólicos/química
Oregon
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Mycolic Acids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001998


  9 / 6400 MEDLINE  
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[PMID]:28806745
[Au] Autor:Gidon A; Åsberg SE; Louet C; Ryan L; Haug M; Flo TH
[Ad] Endereço:Centre of Molecular Inflammation Research and Department of Cancer Research and Molecular Medicine, Faculty of Medicine, NTNU, Norwegian University of Science and Technology, Trondheim, Norway.
[Ti] Título:Persistent mycobacteria evade an antibacterial program mediated by phagolysosomal TLR7/8/MyD88 in human primary macrophages.
[So] Source:PLoS Pathog;13(8):e1006551, 2017 Aug.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pathogenic mycobacteria reside in macrophages where they avoid lysosomal targeting and degradation through poorly understood mechanisms proposed to involve arrest of phagosomal maturation at an early endosomal stage. A clear understanding of how this relates to host defenses elicited from various intracellular compartments is also missing and can only be studied using techniques allowing single cell and subcellular analyses. Using confocal imaging of human primary macrophages infected with Mycobacterium avium (Mav) we show evidence that Mav phagosomes are not arrested at an early endosomal stage, but mature to a (LAMP1+/LAMP2+/CD63+) late endosomal/phagolysosomal stage where inflammatory signaling and Mav growth restriction is initiated through a mechanism involving Toll-like receptors (TLR) 7 and 8, the adaptor MyD88 and transcription factors NF-κB and IRF-1. Furthermore, a fraction of the mycobacteria re-establish in a less hostile compartment (LAMP1-/LAMP2-/CD63-) where they not only evade destruction, but also recognition by TLRs, growth restriction and inflammatory host responses that could be detrimental for intracellular survival and establishment of chronic infections.
[Mh] Termos MeSH primário: Macrófagos/microbiologia
Infecções por Mycobacterium/imunologia
Fator 88 de Diferenciação Mieloide/imunologia
Receptor 7 Toll-Like/imunologia
[Mh] Termos MeSH secundário: Seres Humanos
Processamento de Imagem Assistida por Computador
Imuno-Histoquímica
Lisossomos/imunologia
Macrófagos/imunologia
Microscopia Confocal
Mycobacterium avium
Fagossomos/imunologia
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MYD88 protein, human); 0 (Myeloid Differentiation Factor 88); 0 (TLR7 protein, human); 0 (Toll-Like Receptor 7)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006551


  10 / 6400 MEDLINE  
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[PMID]:28795999
[Au] Autor:Solomon IH; Johncilla ME; Hornick JL; Milner DA
[Ad] Endereço:*Department of Pathology, Brigham and Women's Hospital, Boston, MA †American Society for Clinical Pathology, Chicago, IL.
[Ti] Título:The Utility of Immunohistochemistry in Mycobacterial Infection: A Proposal for Multimodality Testing.
[So] Source:Am J Surg Pathol;41(10):1364-1370, 2017 Oct.
[Is] ISSN:1532-0979
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mycobacterium species are slow growing bacteria that cause significant morbidity and mortality worldwide. Because of the relative rarity of mycobacterial infections, potential for detection of nonpathogenic environmental contaminants, and substantial costs associated with molecular diagnostics, effective screening methods are needed to identify samples most suitable for molecular testing. While anatomic pathology specimens can be utilized to identify characteristic histologic inflammatory patterns and to directly visualize mycobacteria through histochemical (acid fast bacilli [AFB]) stains, the utility of immunohistochemistry (IHC) in this setting is unknown. A cohort of 121 cases previously referred for mycobacterial sequencing, including 12 Mycobacterium tuberculosis (MTB), 42 nontuberculosis mycobacteria (NTM), and 67 cases negative for mycobacteria by polymerase chain reaction (PCR), were stained with an antimycobacteria antibody, and the results were compared with histology, AFB stains, PCR, and cultures. IHC was positive in 50% MTB, 81% NTM, and 49% of cases negative for mycobacteria by sequencing, with excellent (>90%) concordance with AFB stains. Organisms were identifiable by IHC using a 10× objective in the majority of cases. Negative PCR with positive IHC was attributed to paucity of organisms in 30/33 cases, and positive PCR with negative IHC was most often associated with MTB. IHC is highly sensitive for NTM but has a lower sensitivity for MTB, suggesting that cases with a high clinical and histologic suspicion for MTB should be sent for PCR even when AFB and IHC are negative. Incorporation of IHC into a screening algorithm for molecular testing has the potential for significant savings of cost and time.
[Mh] Termos MeSH primário: Infecções por Mycobacterium/diagnóstico
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Imuno-Histoquímica
Masculino
Meia-Idade
Técnicas de Diagnóstico Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1097/PAS.0000000000000925



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