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  1 / 6529 MEDLINE  
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[PMID]:29326266
[Au] Autor:Pace L; Goudot C; Zueva E; Gueguen P; Burgdorf N; Waterfall JJ; Quivy JP; Almouzni G; Amigorena S
[Ad] Endereço:Institut Curie, PSL Research University, F-75005 Paris, France. luigia.pace@iigm.it genevieve.almouzni@curie.fr sebastian.amigorena@curie.fr.
[Ti] Título:The epigenetic control of stemness in CD8 T cell fate commitment.
[So] Source:Science;359(6372):177-186, 2018 Jan 12.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:After priming, naïve CD8 T lymphocytes establish specific heritable transcription programs that define progression to long-lasting memory cells or to short-lived effector cells. Although lineage specification is critical for protection, it remains unclear how chromatin dynamics contributes to the control of gene expression programs. We explored the role of gene silencing by the histone methyltransferase Suv39h1. In murine CD8 T cells activated after infection, Suv39h1-dependent trimethylation of histone H3 lysine 9 controls the expression of a set of stem cell-related memory genes. Single-cell RNA sequencing revealed a defect in silencing of stem/memory genes selectively in -defective T cell effectors. As a result, -defective CD8 T cells show sustained survival and increased long-term memory reprogramming capacity. Thus, Suv39h1 plays a critical role in marking chromatin to silence stem/memory genes during CD8 T effector terminal differentiation.
[Mh] Termos MeSH primário: Linfócitos T CD8-Positivos/imunologia
Linfócitos T CD8-Positivos/metabolismo
Inativação Gênica
Histona-Lisina N-Metiltransferase/metabolismo
Memória Imunológica
Listeriose/imunologia
Metiltransferases/metabolismo
Proteínas Repressoras/metabolismo
[Mh] Termos MeSH secundário: Animais
Diferenciação Celular
Células Cultivadas
Cromatina/metabolismo
Epigênese Genética
Feminino
Histona-Lisina N-Metiltransferase/genética
Histonas/metabolismo
Listeria monocytogenes/imunologia
Masculino
Metilação
Metiltransferases/genética
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Proteínas Repressoras/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin); 0 (Histones); 0 (RNA, Messenger); 0 (Repressor Proteins); EC 2.1.1. (Suv39h1 protein, mouse); EC 2.1.1.- (Methyltransferases); EC 2.1.1.43 (Histone-Lysine N-Methyltransferase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180113
[St] Status:MEDLINE
[do] DOI:10.1126/science.aah6499


  2 / 6529 MEDLINE  
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[PMID]:29253892
[Au] Autor:Akgul A; Al-Janabi N; Das B; Lawrence M; Karsi A
[Ad] Endereço:Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi, United States of America.
[Ti] Título:Small molecules targeting LapB protein prevent Listeria attachment to catfish muscle.
[So] Source:PLoS One;12(12):e0189809, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Listeria monocytogenes is a Gram-positive foodborne pathogen and the causative agent of listeriosis. L. monocytogenes lapB gene encodes a cell wall surface anchor protein, and mutation of this gene causes Listeria attenuation in mice. In this work, the potential role of Listeria LapB protein in catfish fillet attachment was investigated. To achieve this, boron-based small molecules designed to interfere with the active site of the L. monocytogenes LapB protein were developed, and their ability to prevent L. monocytogenes attachment to fish fillet was tested. Results indicated that seven out of nine different small molecules were effective in reducing the Listeria attachment to catfish fillets. Of these, three small molecules (SM3, SM5, and SM7) were highly effective in blocking Listeria attachment to catfish fillets. This study suggests an alternative strategy for reduction of L. monocytogenes contamination in fresh and frozen fish products.
[Mh] Termos MeSH primário: Proteínas de Bactérias/antagonistas & inibidores
Peixes-Gato/microbiologia
Contaminação de Alimentos/análise
Microbiologia de Alimentos
Listeria monocytogenes/fisiologia
Proteínas de Membrana/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Proteínas de Bactérias/química
Boro/química
Domínio Catalítico
Contagem de Colônia Microbiana
Produtos Pesqueiros
Alimentos Congelados
Listeriose
Proteínas de Membrana/química
Ligação Proteica
Alimentos Marinhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Membrane Proteins); N9E3X5056Q (Boron)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171219
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189809


  3 / 6529 MEDLINE  
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[PMID]:29185940
[Au] Autor:Araujo V; Neves E; Silva AC; Martins APL; Brito LC
[Ad] Endereço:1​LEAF - Linking Landscape, Environment, Agriculture and Food /DRAT- Departamento dos Recursos Naturais, Ambiente e Território, Instituto Superior de Agronomia, University of Lisbon, 1349-017 Lisbon, Portugal.
[Ti] Título:Listeria monocytogenes cells under nutrient deprivation showed reduced ability to infect the human intestinal cell line HT-29.
[So] Source:J Med Microbiol;67(1):110-117, 2018 Jan.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: This study aimed to evaluate the effect of two types of stress, cold and nutritional, on the viability and the in vitro virulence of the foodborne pathogenic bacteria Listeria monocytogenes. METHODOLOGY: Ten diverse isolates were kept in phosphate-buffered saline (PBS) at optimal (37 °C) or at refrigeration temperature (7 °C), for 1 and 7 days. The viability of the cells [log colony-forming units (c.f.u.)/ml] and their in vitro virulence, before and after storage in these conditions, were investigated. In vitro virulence (log PFA) was evaluated using the human intestinal epithelial cell line HT-29 in plaque-forming assays (PFAs).Results/Key findings. In general, when compared with the conditions at 37 °C, the exposure at 7 °C for 7 days seemed to increase the resistance of the isolates to nutritional stress. Nutritional stress per se acted significantly to decrease the in vitro virulence of the isolates. After 7 days of nutrient deprivation, whether at optimal or at refrigeration temperature, the majority of the isolates assumed a low-virulence phenotype. CONCLUSION: Our results suggest that when L. monocytogenes are in refrigerated post-processing environments that are unable to support their growth they may increase their resistance to nutritional stress and may decrease their virulence. This should be considered when performing risk assessments for refrigerated ready-to-eat (RTE) foods.
[Mh] Termos MeSH primário: Listeria monocytogenes/fisiologia
Listeriose/microbiologia
Virulência/fisiologia
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Contagem de Colônia Microbiana
Células Epiteliais/fisiologia
Microbiologia de Alimentos/métodos
Células HT29
Seres Humanos
Intestinos/microbiologia
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000648


  4 / 6529 MEDLINE  
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[PMID]:29190284
[Au] Autor:Kortebi M; Milohanic E; Mitchell G; Péchoux C; Prevost MC; Cossart P; Bierne H
[Ad] Endereço:Micalis Institute, Inra, AgroParisTech, Université Paris-Saclay, Equipe Epigénétique et Microbiologie Cellulaire, Jouy-en-Josas, France.
[Ti] Título:Listeria monocytogenes switches from dissemination to persistence by adopting a vacuolar lifestyle in epithelial cells.
[So] Source:PLoS Pathog;13(11):e1006734, 2017 Nov.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Listeria monocytogenes causes listeriosis, a foodborne disease that poses serious risks to fetuses, newborns and immunocompromised adults. This intracellular bacterial pathogen proliferates in the host cytosol and exploits the host actin polymerization machinery to spread from cell-to-cell and disseminate in the host. Here, we report that during several days of infection in human hepatocytes or trophoblast cells, L. monocytogenes switches from this active motile lifestyle to a stage of persistence in vacuoles. Upon intercellular spread, bacteria gradually stopped producing the actin-nucleating protein ActA and became trapped in lysosome-like vacuoles termed Listeria-Containing Vacuoles (LisCVs). Subpopulations of bacteria resisted degradation in LisCVs and entered a slow/non-replicative state. During the subculture of host cells harboring LisCVs, bacteria showed a capacity to cycle between the vacuolar and the actin-based motility stages. When ActA was absent, such as in ΔactA mutants, vacuolar bacteria parasitized host cells in the so-called "viable but non-culturable" state (VBNC), preventing their detection by conventional colony counting methods. The exposure of infected cells to high doses of gentamicin did not trigger the formation of LisCVs, but selected for vacuolar and VBNC bacteria. Together, these results reveal the ability of L. monocytogenes to enter a persistent state in a subset of epithelial cells, which may favor the asymptomatic carriage of this pathogen, lengthen the incubation period of listeriosis, and promote bacterial survival during antibiotic therapy.
[Mh] Termos MeSH primário: Células Epiteliais/metabolismo
Listeria monocytogenes
Listeriose/microbiologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/metabolismo
Linhagem Celular
Citoplasma/metabolismo
Proteínas de Choque Térmico/metabolismo
Proteínas Hemolisinas/metabolismo
Seres Humanos
Proteínas de Membrana/metabolismo
Vacúolos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Heat-Shock Proteins); 0 (Hemolysin Proteins); 0 (Membrane Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006734


  5 / 6529 MEDLINE  
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[PMID]:29176582
[Au] Autor:Radoshevich L; Cossart P
[Ad] Endereço:Institut Pasteur, Unité des Interactions Bactéries-Cellules, Département de Biologie Cellulaire et Infection, F-75015 Paris, France.
[Ti] Título:Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis.
[So] Source:Nat Rev Microbiol;16(1):32-46, 2018 Jan.
[Is] ISSN:1740-1534
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Listeria monocytogenes is a food-borne pathogen responsible for a disease called listeriosis, which is potentially lethal in immunocompromised individuals. This bacterium, first used as a model to study cell-mediated immunity, has emerged over the past 20 years as a paradigm in infection biology, cell biology and fundamental microbiology. In this Review, we highlight recent advances in the understanding of human listeriosis and L. monocytogenes biology. We describe unsuspected modes of hijacking host cell biology, ranging from changes in organelle morphology to direct effects on host transcription via a new class of bacterial effectors called nucleomodulins. We then discuss advances in understanding infection in vivo, including the discovery of tissue-specific virulence factors and the 'arms race' among bacteria competing for a niche in the microbiota. Finally, we describe the complexity of bacterial regulation and physiology, incorporating new insights into the mechanisms of action of a series of riboregulators that are critical for efficient metabolic regulation, antibiotic resistance and interspecies competition.
[Mh] Termos MeSH primário: Interações Hospedeiro-Patógeno
Listeria monocytogenes/fisiologia
Listeriose/etiologia
[Mh] Termos MeSH secundário: Animais
Interações Hospedeiro-Patógeno/genética
Interações Hospedeiro-Patógeno/imunologia
Seres Humanos
Listeria monocytogenes/patogenicidade
Listeriose/metabolismo
Virulência
Fatores de Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Virulence Factors)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1038/nrmicro.2017.126


  6 / 6529 MEDLINE  
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[PMID]:29060962
[Au] Autor:Kuang LH; Gong YH; Su M; Jiang YM
[Ad] Endereço:Clinical Laboratory, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
[Ti] Título:[Clinical analysis of 19 pregnancies complicated listeriosis].
[So] Source:Zhonghua Fu Chan Ke Za Zhi;52(10):657-661, 2017 Oct 25.
[Is] ISSN:0529-567X
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:To analyze the clinical characteristics and perinatal outcomes of listeriosis during pregnancy. From July 2010 to April 2017, 70 131 women delivered in West China Second University Hospital. Nineteen cases were confirmed as listeriosis. The clinical symptoms, laboratory results, pathogens, placenta pathology and perinatal outcomes were analyzed retrospectively. The median age of the 19 cases was 29.7 (19.0-42.0) years old. The median time before diagnosis was 4.8(0.5-19.0) days. The main clinical symptoms at first visits were high fever (17/19), increased white blood cells (18/19), abdominal pain (12/19). Listeria was found in samples of mother's blood (11/19), vaginal secretions (15/19), placenta (1/19), neonatal blood (4/19), neonatal phlegm (5/19) and neonatal ear secretions (1/19), respectively. Inflammation of placenta was identified in all 19 cases. Among the 19 cases, 1 was grade â…  chorioamnionitis, 4 was grade â…¡, 5 was grade â…¢ and 9 was grade â…¥. Only 4 newborn survived after therapy, and others suffered perinatal death, including 8 cases of intrauterine death, 3 cases of miscarriage and 6 cases of treatment failure. Listeriosis has characteristics of acute onset, quick development and high morbidity during pregnancy. The empiric use of antibiotics might not cover listeria. The understanding of listeriosis should be improved.
[Mh] Termos MeSH primário: Morte Fetal/etiologia
Listeria monocytogenes/isolamento & purificação
Listeriose/diagnóstico
Complicações Infecciosas na Gravidez/diagnóstico
[Mh] Termos MeSH secundário: Aborto Espontâneo
Adulto
Antibacterianos/uso terapêutico
China/epidemiologia
Corioamnionite/tratamento farmacológico
Corioamnionite/microbiologia
Feminino
Seres Humanos
Recém-Nascido
Listeriose/sangue
Listeriose/tratamento farmacológico
Listeriose/epidemiologia
Parto
Placenta
Gravidez
Complicações Infecciosas na Gravidez/sangue
Complicações Infecciosas na Gravidez/tratamento farmacológico
Complicações Infecciosas na Gravidez/epidemiologia
Resultado da Gravidez
Estudos Retrospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171025
[St] Status:MEDLINE
[do] DOI:10.3760/cma.j.issn.0529-567X.2017.10.003


  7 / 6529 MEDLINE  
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[PMID]:28951764
[Au] Autor:Fan W; Zhou Y; Li H
[Ad] Endereço:Medical Department, Xiangyang No. 1 People's Hospital Affiliated to Hubei University of Medicine, Xiangyang, Hubei 441000, China.
[Ti] Título:Pathway Interaction Network Analysis Identifies Dysregulated Pathways in Human Monocytes Infected by .
[So] Source:Comput Math Methods Med;2017:3195348, 2017.
[Is] ISSN:1748-6718
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In our study, we aimed to extract dysregulated pathways in human monocytes infected by (LM) based on pathway interaction network (PIN) which presented the functional dependency between pathways. After genes were aligned to the pathways, principal component analysis (PCA) was used to calculate the pathway activity for each pathway, followed by detecting seed pathway. A PIN was constructed based on gene expression profile, protein-protein interactions (PPIs), and cellular pathways. Identifying dysregulated pathways from the PIN was performed relying on seed pathway and classification accuracy. To evaluate whether the PIN method was feasible or not, we compared the introduced method with standard network centrality measures. The pathway of RNA polymerase II pretranscription events was selected as the seed pathway. Taking this seed pathway as start, one pathway set (9 dysregulated pathways) with AUC score of 1.00 was identified. Among the 5 hub pathways obtained using standard network centrality measures, 4 pathways were the common ones between the two methods. RNA polymerase II transcription and DNA replication owned a higher number of pathway genes and DEGs. These dysregulated pathways work together to influence the progression of LM infection, and they will be available as biomarkers to diagnose LM infection.
[Mh] Termos MeSH primário: Listeriose/fisiopatologia
Redes e Vias Metabólicas/genética
Monócitos/microbiologia
Monócitos/patologia
[Mh] Termos MeSH secundário: Perfilação da Expressão Gênica
Regulação da Expressão Gênica
Seres Humanos
Listeria monocytogenes
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE
[do] DOI:10.1155/2017/3195348


  8 / 6529 MEDLINE  
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[PMID]:28945113
[Au] Autor:Liu D; Wang Y; Wang Y; Zhang L; Luo L; Liu K; Ye C
[Ad] Endereço:State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Changping, Changbai Road 155,
[Ti] Título:Development of a Novel Listeria Enrichment Broth for the Isolation of Pathogenic Listeria.
[So] Source:J Food Prot;80(10):1768-1776, 2017 Oct.
[Is] ISSN:1944-9097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Listeriosis, the disease caused by pathogenic Listeria species, can present severe symptoms in susceptible people. The goal of this study was to develop a novel enrichment broth, Listeria allose enrichment broth (LAEB), to improve isolation of Listeria monocytogenes and Listeria ivanovii from samples through incorporating a specific carbohydrate and reducing inhibitor concentrations. Other coexisting bacteria, particularly Listeria innocua, can interfere with the isolation of pathogenic Listeria in such ways as overgrowth of L. innocua and the generation of inhibitory metabolites. The incorporation of allose into the novel LAEB was effective for slowing the growth of L. innocua and other nontarget microorganisms. We determined that 35°C and pH 7.0 under aerobic conditions are optimal for Listeria growth in this medium. The novelty of the use of LAEB is the single enrichment procedure at 35°C for 24 h, obviating the need for a secondary enrichment medium. In 50 simulated samples, the sensitivity of the LAEB method (86%) was higher than that of the International Organization for Standardization (EN ISO) method (70%). In 142 naturally contaminated samples tested, the isolation rate for pathogenic Listeria with the LAEB method was 26.0% (37 of 142 samples), which was significantly higher than the 17.6% (25 of 142 samples) for the EN ISO method. Higher isolation rates and a quicker and easier protocol make the novel LAEB method an appropriate alternative for the isolation of pathogenic Listeria.
[Mh] Termos MeSH primário: Meios de Cultura
Microbiologia de Alimentos
Listeria/isolamento & purificação
[Mh] Termos MeSH secundário: Seres Humanos
Listeria monocytogenes
Listeriose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170926
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-16-529


  9 / 6529 MEDLINE  
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[PMID]:28911869
[Au] Autor:Li J; Lam WW; Lai TW; Au SW
[Ad] Endereço:Center for Protein Science and Crystallography, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China.
[Ti] Título:Degradation of nuclear Ubc9 induced by listeriolysin O is dependent on K efflux.
[So] Source:Biochem Biophys Res Commun;493(2):1115-1121, 2017 Nov 18.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Listeriolysin O (LLO) is a pore-forming toxin produced by L. monocytogenes, and is belonged to a protein family of cholesterol-dependent cytolysins (CDCs). Previous studies have demonstrated that LLO triggers Ubc9 degradation and disrupts host SUMOylation to facilitate bacterial infection. However, the underlying mechanism of Ubc9 degradation is unclear. Here we show that LLO-induced down-regulation of Ubc9 is independent of Ubc9-SUMO interaction, however, it may involve phosphorylation signaling. Additionally, LLO exerts its effects primarily on nuclear Ubc9 and this process is mediated by K efflux. Interestingly, for intracellular CDCs such as pneumolysin and suilysin, blockage of K efflux enhances degradation of nuclear Ubc9, suggesting that extracellular and intracellular pathogens may exploit different mechanisms to modulate host SUMOylation system. Furthermore, up-regulation of SUMOylation by stable expression of SUMO-1 or SUMO-2 shows a delay in membrane perforation by LLO, indicating that SUMO modification of host proteins may act at the frontline for the defense response against LLO. Taken together, our study provides insights to the understanding of host-pathogen interactions.
[Mh] Termos MeSH primário: Toxinas Bacterianas/metabolismo
Proteínas de Choque Térmico/metabolismo
Proteínas Hemolisinas/metabolismo
Listeria monocytogenes/fisiologia
Listeriose/metabolismo
Potássio/metabolismo
Proteólise
Enzimas de Conjugação de Ubiquitina/metabolismo
[Mh] Termos MeSH secundário: Cátions Monovalentes/metabolismo
Células HeLa
Interações Hospedeiro-Patógeno
Seres Humanos
Listeriose/microbiologia
Fosforilação
Sumoilação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Toxins); 0 (Cations, Monovalent); 0 (Heat-Shock Proteins); 0 (Hemolysin Proteins); 72270-41-8 (hlyA protein, Listeria monocytogenes); EC 2.3.2.23 (Ubiquitin-Conjugating Enzymes); EC 6.3.2.- (ubiquitin-conjugating enzyme UBC9); RWP5GA015D (Potassium)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE


  10 / 6529 MEDLINE  
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[PMID]:28898264
[Au] Autor:Rakic Martinez M; Wiedmann M; Ferguson M; Datta AR
[Ad] Endereço:Center for Food Safety and Applied Nutrition, Food and Drug Administration, Laurel, Maryland, United States of America.
[Ti] Título:Assessment of Listeria monocytogenes virulence in the Galleria mellonella insect larvae model.
[So] Source:PLoS One;12(9):e0184557, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Several animal models have been used to understand the molecular basis of the pathogenicity, infectious dose and strain to strain variation of Listeria monocytogenes. The greater wax worm Galleria mellonella, as an alternative model, provides some useful advantages not available with other models and has already been described as suitable for the virulence assessment of various pathogens including L. monocytogenes. The objectives of this study are: 1) confirming the usefulness of this model with a wide panel of Listeria spp. including non-pathogenic L. innocua, L. seeligeri, L. welshimeri and animal pathogen L. ivanovii; 2) assessment of virulence of several isogenic in-frame deletion mutants in virulence and stress related genes of L. monocytogenes and 3) virulence assessment of paired food and clinical isolates of L. monocytogenes from 14 major listeriosis outbreaks occurred worldwide between 1980 and 2015. Larvae injected with different concentrations of Listeria were incubated at 37°C and monitored over seven days for time needed to kill 50% of larvae (LT50) and to determine change of bacterial population in G. mellonella, 2 and 24 hours post-inoculation. Non-pathogenic members of Listeria and L. ivanovii showed significantly (P < 0.05) higher LT50 (lower virulence) than the wild type L. monocytogenes strains. Isogenic mutants of L. monocytogenes with the deletions in prfA, plcA, hly, actA and virR genes, also showed significantly (P < 0.05) higher LT50 than the wild type strain at the inoculum of 106CFU/larva. Food isolates had significantly (P < 0.05) lower virulence than the paired clinical isolates, at all three inoculum concentrations. L. monocytogenes strains related to non-invasive (gastroenteritis) outbreaks of listeriosis showed significantly (P < 0.05) lower virulence than isolates of the same serotype obtained from outbreaks with invasive symptoms. The difference, however, was dose and strain- dependent. No significant differences in virulence were observed among the serotype tested in this study.
[Mh] Termos MeSH primário: Lepidópteros/microbiologia
Listeria monocytogenes/patogenicidade
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Deleção de Genes
Larva/microbiologia
Lepidópteros/crescimento & desenvolvimento
Listeria monocytogenes/genética
Listeriose/microbiologia
Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170913
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184557



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