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[PMID]:29458673
[Au] Autor:Morse DJ; Wilson MJ; Wei X; Lewis MAO; Bradshaw DJ; Murdoch C; Williams DW
[Ad] Endereço:1​Oral and Biomedical Sciences, School of Dentistry, Cardiff University, Cardiff, UK.
[Ti] Título:Denture-associated biofilm infection in three-dimensional oral mucosal tissue models.
[So] Source:J Med Microbiol;67(3):364-375, 2018 Mar.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: In vitro analyses of virulence, pathogenicity and associated host cell responses are important components in the study of biofilm infections. The Candida-related infection, denture-associated oral candidosis, affects up to 60 % of denture wearers and manifests as inflammation of palatal tissues contacting the denture-fitting surface. Commercially available three-dimensional tissue models can be used to study infection, but their use is limited for many academic research institutions, primarily because of the substantial purchase costs. The aim of this study was to develop and evaluate the use of in vitro tissue models to assess infections by biofilms on acrylic surfaces through tissue damage and Candida albicans virulence gene expression. METHODOLOGY: In vitro models were compared against commercially available tissue equivalents (keratinocyte-only, SkinEthic; full-thickness, MatTek Corporation). An in vitro keratinocyte-only tissue was produced using a cancer-derived cell line, TR146, and a full-thickness model incorporating primary fibroblasts and immortalised normal oral keratinocytes was also generated. The in vitro full-thickness tissues incorporated keratinocytes and fibroblasts, and have potential for future further development and analysis. RESULTS: Following polymicrobial infection with biofilms on acrylic surfaces, both in-house developed models were shown to provide equivalent results to the SkinEthic and MatTek models in terms of tissue damage: a significant (P<0.05) increase in LDH activity for mixed species biofilms compared to uninfected control, and no significant difference (P>0.05) in the expression of most C. albicans virulence genes when comparing tissue models of the same type. CONCLUSION: Our results confirm the feasibility and suitability of using these alternative in vitro tissue models for such analyses.
[Mh] Termos MeSH primário: Biofilmes/crescimento & desenvolvimento
Candidíase Bucal/microbiologia
Dentaduras/microbiologia
Interações Hospedeiro-Patógeno
Mucosa Bucal/microbiologia
[Mh] Termos MeSH secundário: Candida albicans/genética
Candida albicans/patogenicidade
Candida albicans/fisiologia
Linhagem Celular
Coinfecção/microbiologia
Fibroblastos/microbiologia
Seres Humanos
Queratinócitos/microbiologia
Polimetil Metacrilato
Estomatite sob Prótese
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9011-14-7 (Polymethyl Methacrylate)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000677


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[PMID]:28457069
[Au] Autor:Kriem S; Peretz A; Blum A
[Ad] Endereço:Department of Medicine, Padeh Poria Medical Center, Tiberias, affiliated with Faculty of Medicine in the Galilee, Bar Ilan University, Safed, Israel.
[Ti] Título:Lingua Villosa Nigra.
[So] Source:Isr Med Assoc J;19(2):131, 2017 Feb.
[Is] ISSN:1565-1088
[Cp] País de publicação:Israel
[La] Idioma:eng
[Mh] Termos MeSH primário: Candida albicans/isolamento & purificação
Candidíase Bucal
Fluconazol/administração & dosagem
Língua Pilosa
[Mh] Termos MeSH secundário: Administração Tópica
Idoso de 80 Anos ou mais
Antifúngicos/administração & dosagem
Candidíase Bucal/complicações
Candidíase Bucal/diagnóstico
Candidíase Bucal/tratamento farmacológico
Candidíase Bucal/fisiopatologia
Feminino
Seres Humanos
Língua Pilosa/diagnóstico
Língua Pilosa/tratamento farmacológico
Língua Pilosa/etiologia
Língua Pilosa/fisiopatologia
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 8VZV102JFY (Fluconazole)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171130
[Lr] Data última revisão:
171130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170501
[St] Status:MEDLINE


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[PMID]:29025965
[Au] Autor:Hatipoglu N; Güvenç BH; Deswarte C; Koksalan K; Boisson-Dupuis S; Casanova JL; Bustamante J
[Ad] Endereço:Pediatrics Unit and naydin9@mynet.com.
[Ti] Título:Inherited IL-12Rß1 Deficiency in a Child With BCG Adenitis and Oral Candidiasis: A Case Report.
[So] Source:Pediatrics;140(5), 2017 Nov.
[Is] ISSN:1098-4275
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tuberculosis is a major worldwide problem, and protection from it is achieved mainly by live attenuated bacille Calmette-Guérin vaccine, which is capable of causing disease in immunocompromised host. Oral thrush is abnormal in healthy children, which suggests an underlying immunodeficiency. Mendelian susceptibility to mycobacterial disease is a rare primary immunodeficiency characterized by a selective predisposition to weakly virulent and and also predisposition to chronic mucocutaneous candidiasis. Interleukin 12 receptor ß1 (IL-12Rß1) deficiency is the most common disease of Mendelian susceptibility to mycobacterial disease, and to date only 50 IL-12Rß1 deficient patients with clinical signs of chronic mucocutaneous candidiasis have been reported. We report a 2.5-year-old daughter of consanguineous parents with both regional bacille Calmette-Guérin lymphadenitis and recurrent oral candidiasis carrying biallelic R175W mutation in the gene, resulting in complete loss of expression of IL-12Rß1. To our knowledge, this is the first report of bacille Calmette-Guérin lymphadenitis with concurrent oral candidiasis displaying such a mutation. New mutations and wide clinical diversities are the indisputable fact of populations with a high rate of consanguineous marriages.
[Mh] Termos MeSH primário: Vacina BCG/efeitos adversos
Candidíase Bucal/diagnóstico por imagem
Linfadenite/diagnóstico por imagem
Receptores de Interleucina-12/deficiência
[Mh] Termos MeSH secundário: Candidíase Bucal/genética
Pré-Escolar
Feminino
Seres Humanos
Linfadenite/induzido quimicamente
Linfadenite/complicações
Linfadenite/genética
Linhagem
Receptores de Interleucina-12/genética
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BCG Vaccine); 0 (IL12RB1 protein, human); 0 (Receptors, Interleukin-12)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171014
[St] Status:MEDLINE


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[PMID]:28683959
[Au] Autor:Proust-Lemoine E; Guyot S
[Ad] Endereço:Endocrinologie, polyclinique Aguilera, 21, rue de l'Estagnas, 64200 Biarritz, France. Electronic address: e.proustlemoine@yahoo.fr.
[Ti] Título:[Oral diseases in auto-immune polyendocrine syndrome type 1].
[Ti] Título:Polyendocrinopathies auto-immunes de type 1 et pathologies buccales..
[So] Source:Presse Med;46(9):853-863, 2017 Sep.
[Is] ISSN:2213-0276
[Cp] País de publicação:France
[La] Idioma:fre
[Ab] Resumo:Auto-immune polyendocrine syndrome type 1 (APS1) also called Auto-immune Polyendocrinopathy Candidiasis Ectodermal Dystrophy (APECED) is a rare monogenic childhood-onset auto-immune disease. This autosomal recessive disorder is caused by mutations in the auto-immune regulator (AIRE) gene, and leads to autoimmunity targeting peripheral tissues. There is a wide variability in clinical phenotypes in patients with APSI, with auto-immune endocrine and non-endocrine disorders, and chronic mucocutaneous candidiasis. These patients suffer from oral diseases such as dental enamel hypoplasia and candidiasis. Both are frequently described, and in recent series, enamel hypoplasia and candidiasis are even the most frequent components of APS1 together with hypoparathyroidism. Both often occur during childhood (before 5 years old for canrdidiasis, and before 15 years old for enamel hypoplasia). Oral candidiasis is recurrent all life long, could become resistant to azole antifungal after years of treatment, and be carcinogenic, leading to severe oral squamous cell carcinoma. Oral components of APS1 should be diagnosed and rigorously treated. Dental enamel hypoplasia and/or recurrent oral candidiasis in association with auto-immune diseases in a young child should prompt APS1 diagnosis.
[Mh] Termos MeSH primário: Doenças da Boca/diagnóstico
Poliendocrinopatias Autoimunes/diagnóstico
Odontopatias/diagnóstico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Candidíase Bucal/diagnóstico
Candidíase Bucal/genética
Criança
Pré-Escolar
Análise Mutacional de DNA
Hipoplasia do Esmalte Dentário/diagnóstico
Hipoplasia do Esmalte Dentário/genética
Diagnóstico Diferencial
Genes Recessivos/genética
Seres Humanos
Lactente
Doenças da Boca/genética
Fenótipo
Poliendocrinopatias Autoimunes/classificação
Poliendocrinopatias Autoimunes/genética
Odontopatias/genética
Fatores de Transcrição/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (APECED protein); 0 (Transcription Factors)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170708
[St] Status:MEDLINE


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[PMID]:28678952
[Au] Autor:Badaró MM; Salles MM; Leite VMF; Arruda CNF; Oliveira VC; Nascimento CD; Souza RF; Paranhos HFO; Silva-Lovato CH
[Ad] Endereço:Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Materiais Dentários e Prótese, Ribeirão Preto, SP, Brazil.
[Ti] Título:Clinical trial for evaluation of Ricinus communis and sodium hypochlorite as denture cleanser.
[So] Source:J Appl Oral Sci;25(3):324-334, 2017 May-Jun.
[Is] ISSN:1678-7765
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Objective: This study evaluated Ricinus communis and sodium hypochlorite solutions in terms of biofilm removal ability, remission of candidiasis, antimicrobial activity, and participant satisfaction. Material and Methods: It was conducted a controlled clinical trial, randomized, double-blind, and crossover. Sixty-four denture wearers with (n=24) and without candidiasis (n=40) were instructed to brush (3 times/day) and immerse their dentures (20 min/day) in different storage solutions (S1 / S2: 0.25% / 0.5% sodium hypochlorite; S3: 10% R. communis; S4: Saline).The trial period for each solution was seven days and a washout period of seven days was used before starting the use of another solution. The variables were analyzed at baseline and after each trial period. The biofilm of inner surfaces of maxillary dentures was disclosed, photographed, and total and dyed areas were measured (Image Tool software). The percentage of biofilm was calculated. Remission of candidiasis was assessed by visual scale and score were attributed. Antimicrobial activity was assessed by the DNA-Checkerboard hybridization method. Patient satisfaction was measured using a questionnaire. Results: S1 (4.41±7.98%) and S2 (2.93±5.23%) were more effective then S3 (6.95±10.93%) in biofilm remotion(P<0.0001). All solutions were different from the control (11.07±11.99%). S3 was the most effective solution in remission of candidiasis (50%), followed by S1 (46%). Concerning antimicrobial action, S1/S2 were similar and resulted in the lowest microorganism mean count (P=0.04), followed by S3. No significant differences were found with patient's satisfaction. Conclusions: 10% R. communis and 0.25% sodium hypochlorite were effective in biofilm removal, causing remission of candidiasis and reducing the formation of microbial colonies in denture surfaces. All solutions were approved by patients.
[Mh] Termos MeSH primário: Anti-Infecciosos Locais
Biofilmes/efeitos dos fármacos
Candidíase Bucal/tratamento farmacológico
Higienizadores de Dentadura
Prótese Total Superior/microbiologia
Ricinus/química
Hipoclorito de Sódio
[Mh] Termos MeSH secundário: Idoso
Análise de Variância
Contagem de Colônia Microbiana
Método Duplo-Cego
Feminino
Seres Humanos
Modelos Logísticos
Masculino
Satisfação do Paciente
Reprodutibilidade dos Testes
Propriedades de Superfície
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Anti-Infective Agents, Local); 0 (Denture Cleansers); DY38VHM5OD (Sodium Hypochlorite)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170706
[St] Status:MEDLINE


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[PMID]:28604075
[Au] Autor:Vértes L
[Ti] Título:[Szakácsi József Csorba MD (1789-1858), the hospital founder and academician].
[Ti] Título:Szakácsi Dr. Csorba József (1789­1858) megyeikórház-alapító és akadémikus..
[So] Source:Orv Hetil;158(24):956-958, 2017 Jun.
[Is] ISSN:0030-6002
[Cp] País de publicação:Hungary
[La] Idioma:hun
[Mh] Termos MeSH primário: Candidíase Bucal/história
Docentes de Medicina/história
[Mh] Termos MeSH secundário: Academias e Institutos/história
História do Século XVIII
História do Século XIX
Seres Humanos
Hungria
Saúde Pública
[Pt] Tipo de publicação:BIOGRAPHY; HISTORICAL ARTICLE; JOURNAL ARTICLE
[Ps] Nome de pessoa como assunto:Csorba J
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE
[do] DOI:10.1556/650.2017.HO2569


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Machado, Alcyone Artioli
Texto completo SciELO Brasil
[PMID]:28591257
[Au] Autor:Lourenço AG; Ribeiro AERA; Nakao C; Motta ACF; Antonio LGL; Machado AA; Komesu MC
[Ad] Endereço:Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Morfologia, Fisiologia e Patologia Básica, Ribeirão Preto, São Paulo, Brazil.
[Ti] Título:Oral Candida spp carriage and periodontal diseases in HIV-infected patients in Ribeirão Preto, Brazil.
[So] Source:Rev Inst Med Trop Sao Paulo;59:e29, 2017 Jun 01.
[Is] ISSN:1678-9946
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The majority of HIV-infected patients develop Candida spp-associated clinical oral lesions. Studies have shown that asymptomatic oral colonization of Candida spp may lead to oral lesions or become a source of disseminated infections. The aim of this study was to verify the effects of periodontal conditions on Candida spp prevalence and Candida spp carriage in the oral cavity of HIV-infected patients compared to non-infected patients. Twenty-five patients not infected with HIV and 48 HIV-infected patients were classified according to periodontal conditions as being periodontal healthy or with periodontal disease. Candida spp carriage and classification were performed in oral rinse samples. Viral load and CD4+ T lymphocyte (CD4+L) counts were performed in blood samples from HIV-infected patients. No differences in Candida spp prevalence related to HIV status or periodontal condition were detected. However, Candida spp carriage was increased in periodontally affected HIV-infected patients when compared to periodontally healthy HIV-infected patients (p= 0.04). Periodontally healthy HIV-infected patients presented Candida spp carriage in similar levels as healthy or periodontally affected non-HIV-infected patients. Candida spp carriage was correlated with CD4+L counting in HIV-infected patients. We concluded that periodontal disease is associated with increased Candida spp carriage in HIV-infected patients and may be a predisposing factor to clinical manifestations of candidiasis.
[Mh] Termos MeSH primário: Infecções Oportunistas Relacionadas com a AIDS/microbiologia
Candida/classificação
Candidíase Bucal/microbiologia
Doenças Periodontais/microbiologia
[Mh] Termos MeSH secundário: Infecções Oportunistas Relacionadas com a AIDS/epidemiologia
Adulto
Brasil/epidemiologia
Contagem de Linfócito CD4
Candida/isolamento & purificação
Candidíase Bucal/epidemiologia
Portador Sadio
Feminino
Seres Humanos
Masculino
Boca/microbiologia
Doenças Periodontais/epidemiologia
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE


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[PMID]:28566666
[Au] Autor:Sakaguchi H
[Ad] Endereço:Director of Dental Practice, Ryohoku Hospital.
[Ti] Título:Treatment and Prevention of Oral Candidiasis in Elderly Patients.
[So] Source:Med Mycol J;58(2):J43-J49, 2017.
[Is] ISSN:1882-0476
[Cp] País de publicação:Japan
[La] Idioma:jpn
[Ab] Resumo:The incidence of oral candidiasis has increased in the elderly in recent years. Although the increase of the elderly population plays a big role in this rise of oral candidiasis, the broader recognition that elderly people have higher infection rates for oral candidiasis is considered to be also an important factor. Oral candidiasis can be categorized into three types. Pseudomembranous oral candidiasis is characterized by the appearance of white moss, erythematous oral candidiasis by the eruption of erythema, and hyperplastic oral candidiasis by mucosal hyperplasia. Miconazole has been commonly used when treating oral candidiasis. Elderly patients, however, have a tendency to develop oral candidiasis repeatedly. It is therefore critical to take measures to prevent recurrence. We recommend the use an oral moisturizer containing hinokitiol, an antifungal substance, on a regular basis, to help prevent recurrence of oral candidiasis.
[Mh] Termos MeSH primário: Candidíase Bucal/tratamento farmacológico
Candidíase Bucal/prevenção & controle
[Mh] Termos MeSH secundário: Idoso
Idoso de 80 Anos ou mais
Antifúngicos/administração & dosagem
Candidíase Bucal/classificação
Candidíase Bucal/patologia
Quimioterapia Combinada
Feminino
Seres Humanos
Masculino
Miconazol/administração & dosagem
Monoterpenos/administração & dosagem
Recidiva
Prevenção Secundária
Tropolona/administração & dosagem
Tropolona/análogos & derivados
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Monoterpenes); 7L6DL16P1T (Tropolone); 7NNO0D7S5M (Miconazole); U5335D6EBI (beta-thujaplicin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171015
[Lr] Data última revisão:
171015
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE
[do] DOI:10.3314/mmj.17.004


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[PMID]:28566081
[Au] Autor:Eryildiz C; Bukavaz S; Gürcan S; Hatipoglu O
[Ad] Endereço:Trakya University Faculty of Medicine, Department of Medical Microbiology, Edirne, Turkey. cananeryildiz@gmail.com.
[Ti] Título:[A rarely isolated bacterium in microbiology laboratories: Streptococcus uberis].
[Ti] Título:Mikrobiyoloji laboratuvarlarinda nadir izole edilen bir etken: Streptococcus uberis..
[So] Source:Mikrobiyol Bul;51(2):171-176, 2017 Apr.
[Is] ISSN:0374-9096
[Cp] País de publicação:Turkey
[La] Idioma:tur
[Ab] Resumo:Streptococcus uberis is a gram-positive bacterium that is mostly responsible for mastitis in cattle. The bacterium rarely has been associated with human infections. Conventional phenotyphic methods can be inadequate for the identification of S.uberis; and in microbiology laboratories S.uberis is confused with the other streptococci and enterococci isolates. Recently, molecular methods are recommended for the accurate identification of S.uberis isolates. The aim of this report is to present a lower respiratory tract infection case caused by S.uberis and the microbiological methods for identification of this bacterium. A 66-year-old male patient with squamous cell lung cancer who received radiotherapy was admitted in our hospital for the control. According to the chest X-Ray, patient was hospitalized with the prediagnosis of ''cavitary tumor, pulmonary abscess''. In the first day of the hospitalization, blood and sputum cultures were drawn. Blood culture was negative, however, Candida albicans was isolated in the sputum culture and it was estimated to be due to oral lesions. After two weeks from the hospitalization, sputum sample was taken from the patient since he had abnormal respiratory sounds and cough complaint. In the Gram stained smear of the sputum there were abundant leucocytes and gram-positive cocci, and S.uberis was isolated in both 5% sheep blood and chocolate agar media. Bacterial identification and antibiotic susceptibility tests were performed by VITEK 2 (Biomerieux, France) and also, the bacterium was identified by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) based VITEK MS system as S.uberis. The isolate was determined susceptible to ampicillin, erythromycin, clindamycin, levofloxacin, linezolid, penicillin, cefotaxime, ceftriaxone, tetracycline and vancomycin. 16S, 23S ribosomal RNA and 16S-23S intergenic spacer gene regions were amplified with specific primers and partial DNA sequence analysis of 16S rRNA polymerase chain reaction (PCR) products were performed by 3500xL Genetic Analyzer (Applied Biosystems, USA). According to the partial 16S rRNA gene sequencing results, bacterium was confirmed as S.uberis. This report makes a significant contribution to the number of case reports of human infections caused by S.uberis as the identification was performed by current microbiological methods in our case. In conclusion, S.uberis should be evaluated as an opportunistic pathogen among the immunosuppressed patients and in addition to phenotypic bacteriological methods, the other recent microbiological methods should also be utilized for the identification.
[Mh] Termos MeSH primário: Infecções Oportunistas/microbiologia
Infecções Estreptocócicas/microbiologia
Streptococcus/classificação
Streptococcus/isolamento & purificação
[Mh] Termos MeSH secundário: Idoso
Candida albicans/isolamento & purificação
Candidíase Bucal/complicações
Candidíase Bucal/microbiologia
Carcinoma de Células Escamosas/complicações
Seres Humanos
Neoplasias Pulmonares/complicações
Masculino
Testes de Sensibilidade Microbiana
Infecções Oportunistas/complicações
Reação em Cadeia da Polimerase
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Escarro/microbiologia
Infecções Estreptocócicas/complicações
Streptococcus/efeitos dos fármacos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE


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[PMID]:28423089
[Au] Autor:Livério HO; Ruiz LDS; Freitas RS; Nishikaku A; Souza AC; Paula CR; Domaneschi C
[Ad] Endereço:Universidade de São Paulo, Faculdade de Odontologia, Departamento de Estomatologia, São Paulo, São Paulo, Brazil.
[Ti] Título:Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients.
[So] Source:Rev Inst Med Trop Sao Paulo;59:e14, 2017 Apr 13.
[Is] ISSN:1678-9946
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to assess a collection of yeasts to verify the presence of Candida dubliniensis among strains isolated from the oral mucosa of AIDS pediatric patients which were initially characterized as Candida albicans by the traditional phenotypic method, as well as to evaluate the main phenotypic methods used in the discrimination between the two species and confirm the identification through genotypic techniques, i.e., DNA sequencing. Twenty-nine samples of C. albicans isolated from this population and kept in a fungi collection were evaluated and re-characterized. In order to differentiate the two species, phenotypic tests (Thermotolerance tests, Chromogenic medium, Staib agar, Tobacco agar, Hypertonic medium) were performed and genotypic techniques using DNA sequencing were employed for confirmation of isolated species. Susceptibility and specificity were calculated for each test. No phenotypic test alone was sufficient to provide definitive identification of C. dubliniensis or C. albicans, as opposed to results of molecular tests. After amplification and sequencing of specific regions of the 29 studied strains, 93.1% of the isolates were identified as C. albicans and 6.9% as C. dubliniensis. The Staib agar assay showed a higher susceptibility (96.3%) in comparison with other phenotypic techniques. Therefore, genotypic methods are indispensable for the conclusive identification and differentiation between these species.
[Mh] Termos MeSH primário: Infecções Oportunistas Relacionadas com a AIDS/microbiologia
Candida/genética
Candidíase Bucal/microbiologia
DNA Fúngico/genética
[Mh] Termos MeSH secundário: Candida/classificação
Candida/isolamento & purificação
Candida albicans/genética
Candida albicans/isolamento & purificação
Criança
Genótipo
Seres Humanos
Mucosa Bucal/microbiologia
Técnicas de Tipagem Micológica
Fenótipo
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE



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