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  1 / 3115 MEDLINE  
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[PMID]:29190763
[Au] Autor:Marangoni A; Foschi C; Micucci M; Nahui Palomino RA; Gallina Toschi T; Vitali B; Camarda L; Mandrioli M; De Giorgio M; Aldini R; Corazza I; Chiarini A; Cevenini R; Budriesi R
[Ad] Endereço:Department of Specialized, Experimental, and Diagnostic Medicine (DIMES), Operative Unit of Clinical Microbiology, St. Orsola-Malpighi University Hospital, Alma Mater Studiorum-University of Bologna, Bologna, Italy.
[Ti] Título:In vitro activity of Spirulina platensis water extract against different Candida species isolated from vulvo-vaginal candidiasis cases.
[So] Source:PLoS One;12(11):e0188567, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The high incidence of vulvo-vaginal candidiasis, combined with the growing problems about azole resistance and toxicity of antifungal drugs, highlights the need for the development of new effective strategies for the treatment of this condition. In this context, natural compounds represent promising alternatives. The cyanobacterium Spirulina platensis, a blue-green alga, exhibits antimicrobial activities against several microorganisms. Nevertheless, only few data about the antifungal properties of Spirulina platensis are available and its potential toxic effects have not been largely investigated. The aim of this study was to evaluate the in vitro activity of a fully-characterized water extract of Spirulina platensis against 22 strains of Candida spp. Prior to considering its potential topical use, we both investigated whether the extract exerted target activities on guinea pig uterine smooth muscle, and the impact of Spirulina platensis on the dominant microorganisms of the vaginal microbiota (i.e., lactobacilli), in order to exclude possible adverse events. By means of a broth microdilution assay, we found that the microalga extract possesses good antifungal properties (MIC: 0.125-0.5 mg/ml), against all the Candida species with a fungicidal activity. At the concentrations active against candida, Spirulina platensis did not modify the spontaneous basic waves pattern of uterine myometrium as underlined by the absence of aberrant contractions, and did not affect the main health-promoting bacteria of the vaginal ecosystem. Finally, we evaluated the selectivity index of our extract by testing its cytotoxicity on three different cell lines and it showed values ranging between 2 and 16. Further in vivo studies are needed, in particular to evaluate the use of control-release formulations in order to maintain Spirulina platensis concentrations at anti-Candida active doses but below the toxic levels found in the present work.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Candida/efeitos dos fármacos
Candidíase Vulvovaginal/microbiologia
Spirulina/química
Água/química
[Mh] Termos MeSH secundário: Animais
Candida/isolamento & purificação
Feminino
Cobaias
Seres Humanos
Técnicas In Vitro
Testes de Sensibilidade Microbiana
Microbiota
Contração Uterina/efeitos dos fármacos
Vagina/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 059QF0KO0R (Water)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188567


  2 / 3115 MEDLINE  
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Svidzinski, Terezinha Inez Estivalet
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[PMID]:28771140
[Au] Autor:Faria DR; Sakita KM; Akimoto-Gunther LS; Kioshima ÉS; Svidzinski TIE; Bonfim-Mendonça PS
[Ad] Endereço:Division of Medical Mycology, Teaching and Research Laboratory in Clinical Analyses, Department of Clinical Analysis of State University of Maringá, Paraná, Brazil.
[Ti] Título:Cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies.
[So] Source:J Med Microbiol;66(8):1225-1228, 2017 Aug.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study aimed to characterize cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies. It was evaluated 12 clinical isolates of C. albicans from vaginal samples: 4 from asymptomatic women (AS), 4 from women with a single episode of vulvovaginal candidiasis (VVC) and 4 from women with recurrent vulvovaginal candidiasis (RVVC). We evaluated the ability of C. albicans to adhere to human cervical cancer cells (SiHa), the yeast-SiHa cell interactions and cell damage. All of the clinical isolates presented a high adhesion capacity on SiHa cells. However, clinical isolates from symptomatic women (VVC and RVVC) had higher filamentation after contact (24 h) with SiHa cells and a greater capacity to cause cell damage (>80 %). Clinical isolates from symptomatic women had greater potential to invade SiHa cells, suggesting that they are more pathogenic than AS isolates.
[Mh] Termos MeSH primário: Candida albicans/isolamento & purificação
Candidíase Vulvovaginal/diagnóstico
Candidíase Vulvovaginal/microbiologia
[Mh] Termos MeSH secundário: Candida albicans/classificação
Candida albicans/genética
Candidíase Vulvovaginal/fisiopatologia
Morte Celular
Linhagem Celular Tumoral
Feminino
Seres Humanos
Vagina/citologia
Vagina/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000547


  3 / 3115 MEDLINE  
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[PMID]:28594779
[Au] Autor:Gaydos CA; Beqaj S; Schwebke JR; Lebed J; Smith B; Davis TE; Fife KH; Nyirjesy P; Spurrell T; Furgerson D; Coleman J; Paradis S; Cooper CK
[Ad] Endereço:Johns Hopkins University, Baltimore, Maryland; Pathology Inc, Torrance, California; the University of Alabama at Birmingham, Birmingham, Alabama; Planned Parenthood Southeastern Pennsylvania, Philadelphia, Pennsylvania; Planned Parenthood Gulf Coast, Houston, Texas; Sidney and Lois Eskenazi Hospital and Indiana University School of Medicine, Indianapolis, Indiana; Drexel University College of Medicine, Philadelphia, Pennsylvania; Planned Parenthood of Southern New England, New Haven, Connecticut; Planned Parenthood Mar Monte, San Jose, California; and BD Diagnostics, Québec, Canada, and Sparks, Maryland.
[Ti] Título:Clinical Validation of a Test for the Diagnosis of Vaginitis.
[So] Source:Obstet Gynecol;130(1):181-189, 2017 Jul.
[Is] ISSN:1873-233X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Vaginitis may be diagnosed as bacterial vaginosis, vulvovaginal candidiasis, trichomoniasis, or coinfection. A new molecular test assays the vaginal microbiome and organisms that cause three common infections. The objective of the trial was to evaluate the clinical accuracy of the investigational test for vaginal swabs collected by patients (self) or clinicians. The primary and secondary outcomes were to compare the investigational test with reference methods for the three most common causes of vaginitis and compare clinician-collected with self-collected swabs. METHODS: We conducted a cross-sectional study in which women with symptoms of vaginitis were recruited at ten clinical centers and consented to the investigation between May and September 2015. The woman collected a vaginal swab, sheathed, and then handed it to the clinician. These swabs were to evaluate how self-collected swabs compared with clinician-collected swabs. The clinician collected an investigational test swab and reference test swabs. From 1,740 symptomatic patients, clinician-collected and self-collected vaginal swabs were evaluated by the molecular test and six tests. The reference methods for bacterial vaginosis were Nugent's score and Amsel's criteria for intermediate Nugent results. The reference methods for Candida infection were isolation of any potential Candida microorganisms from inoculation of two culture media: chromogenic and Sabouraud agar and sequencing. The reference methods for trichomoniasis were wet mount and culture. RESULTS: For clinician-collected swabs, by reference methods, bacterial vaginosis was diagnosed in 56.5%, vaginal candidiasis in 32.8%, trichomoniasis in 8%, and none of the three infections in 24% with a coinfection rate of 20%. The investigational test sensitivity was 90.5% (95% confidence interval [CI] 88.3-92.2%) and specificity was 85.8% (95% CI 83.0-88.3%) for bacterial vaginosis. The investigational test sensitivity was 90.9% (95% CI 88.1-93.1%) and specificity was 94.1% (95% CI 92.6-95.4%) for the Candida group. Sensitivity for Candida glabrata was 75.9% (95% CI 57.9-87.8%) and specificity was 99.7% (95% CI 99.3-99.9%). Investigational test sensitivity was 93.1% (95% CI 87.4-96.3%) and specificity was 99.3% (95% CI 98.7-99.6%) for trichomoniasis. Results from self-collected swabs were similar to clinician-collected swabs. CONCLUSION: A molecular-based test using vaginal swabs collected by clinicians or patients can accurately diagnose most common bacterial, fungal, and protozoan causes of vaginitis. Women and their clinicians seeking accurate diagnosis and appropriate selection of efficacious treatment for symptoms of vaginitis might benefit from this molecular test.
[Mh] Termos MeSH primário: Esfregaço Vaginal/normas
Vaginite/diagnóstico
[Mh] Termos MeSH secundário: Adolescente
Adulto
Candida/isolamento & purificação
Candidíase Vulvovaginal/complicações
Candidíase Vulvovaginal/diagnóstico
Feminino
Seres Humanos
Lactobacillus/isolamento & purificação
Meia-Idade
Valor Preditivo dos Testes
Tricomoníase/complicações
Tricomoníase/diagnóstico
Trichomonas vaginalis/isolamento & purificação
Estados Unidos
Vaginite/microbiologia
Vaginose Bacteriana/complicações
Vaginose Bacteriana/diagnóstico
Adulto Jovem
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; MULTICENTER STUDY
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1097/AOG.0000000000002090


  4 / 3115 MEDLINE  
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[PMID]:28299831
[Au] Autor:Romero-Cerecero O; Islas-Garduño AL; Zamilpa A; Tortoriello J
[Ad] Endereço:Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social (CIBIS-IMSS), Xochitepec, Morelos, Mexico.
[Ti] Título:Effectiveness of Ageratina pichinchensis Extract in Patients with Vulvovaginal Candidiasis. A Randomized, Double-Blind, and Controlled Pilot Study.
[So] Source:Phytother Res;31(6):885-890, 2017 Jun.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Previous clinical studies have demonstrated the antifungal effectiveness of Ageratina pichinchensis extracts when topically administered to patients with dermatomycosis. The objective of this study was to evaluate the effectiveness and tolerability of a 7% standardized extract of A. pichinchensis (intravaginal) in patients with vulvovaginal candidiasis. The extract was standardized in terms of its encecalin content and administered during 6 days to patients with Candida albicans-associated vulvovaginitis. The positive control group was treated with Clotrimazole (100 mg). On day 7 of the study, a partial evaluation was carried out; it demonstrated that 94.1% of patients treated with Clotrimazole and 100% of those treated with the A. pichinchensis extract referred a decrease or absence of signs and symptoms consistent with vulvovaginal candidiasis. In the final evaluation, 2 weeks after concluding administration, 86.6% of patients in the control group and 81.2% (p = 0.65) of those treated with the A. pichinchensis extract demonstrated therapeutic success. Statistical analysis evidenced no significant differences between the two treatment groups. With the results obtained, it is possible to conclude that the standardized extract from A. pichinchensis, intravaginally administered, showed therapeutic and mycological effectiveness, as well as tolerability, in patients with vulvovaginal candidiasis, without noting statistical differences in patients treated with Clotrimazole. Copyright © 2017 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Ageratina/química
Antifúngicos/uso terapêutico
Candidíase Vulvovaginal/tratamento farmacológico
Extratos Vegetais/uso terapêutico
[Mh] Termos MeSH secundário: Adulto
Candida albicans/efeitos dos fármacos
Clotrimazol/uso terapêutico
Método Duplo-Cego
Feminino
Seres Humanos
Fitoterapia/métodos
Projetos Piloto
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Plant Extracts); G07GZ97H65 (Clotrimazole)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5802


  5 / 3115 MEDLINE  
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[PMID]:28299777
[Au] Autor:Matheson A; Mazza D
[Ad] Endereço:Department of General Practice, School of Primary Healthcare, Monash University, Notting Hil, Victoria, Australia.
[Ti] Título:Recurrent vulvovaginal candidiasis: A review of guideline recommendations.
[So] Source:Aust N Z J Obstet Gynaecol;57(2):139-145, 2017 Apr.
[Is] ISSN:1479-828X
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Recurrent vulvovaginal candidiasis (VVC) is a difficult-to-manage condition that affects 5-8% of women of reproductive age. Current treatment regimes have high relapse rates, resulting in poor quality of life for the women affected. AIM: To compare the quality and content of current guidelines concerned with recurrent VVC and to develop a summary of recommendations to assist in the management of women with this condition. METHODS: Relevant clinical guidelines were identified through a search of several databases (MEDLINE, SCOPUS and The Cochrane Library) and relevant websites. Five guidelines were identified. Each guideline was assessed for quality using the AGREE II instrument. Guideline recommendations were extracted, compared and contrasted. RESULTS: The identified guidelines were of mixed quality. This is not related to the level of evidence supporting them but is because of poor stakeholder involvement, applicability and lack of clarity concerning editorial independence. Current international guidelines for recurrent VVC are consistent in terms of their definition of the condition, diagnostic techniques and utilising induction and maintenance therapy as the treatment of choice. However, the regimen suggested by most guidelines (fluconazole weekly for six months) is not particularly effective; only 42.9% of patients are disease free after 12 months. An alternative regimen put forward by one of the guidelines cites a 77% cure rate after 12 months. Most guidelines lacked specific recommendations for the induction part of induction and maintenance treatment. CONCLUSION: The current most recommended treatment of recurrent VVC is sub-optimal. Studies performed on a larger scale are required to identify more effective treatments.
[Mh] Termos MeSH primário: Antifúngicos/uso terapêutico
Candidíase Vulvovaginal/diagnóstico
Candidíase Vulvovaginal/tratamento farmacológico
Guias de Prática Clínica como Assunto/normas
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Quimioterapia de Manutenção
Recidiva
Indução de Remissão
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antifungal Agents)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1111/ajo.12592


  6 / 3115 MEDLINE  
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[PMID]:28292981
[Au] Autor:Yano J; Noverr MC; Fidel PL
[Ad] Endereço:Department of Oral and Craniofacial Biology, Louisiana State University Health Sciences Center School of Dentistry, New Orleans, Louisiana, USA.
[Ti] Título:Vaginal Heparan Sulfate Linked to Neutrophil Dysfunction in the Acute Inflammatory Response Associated with Experimental Vulvovaginal Candidiasis.
[So] Source:MBio;8(2), 2017 Mar 14.
[Is] ISSN:2150-7511
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite acute inflammation by polymorphonuclear neutrophils (PMNs) during vulvovaginal candidiasis (VVC), clearance of fails to occur. The purpose of this study was to uncover the mechanism of vaginal PMN dysfunction. Designs included assessing PMN migration, proinflammatory mediators, and tissue damage (by analysis of the activity of lactate dehydrogenase [LDH]) in mice susceptible (C3H/HeN-C57BL/6) or resistant (CD-1) to chronic VVC (CVVC-S or CVVC-R) and testing morphology-specific strains under conditions of preinduced PMN migration (CVVC-S mice) or PMN depletion (CVVC-R mice). designs included evaluation of killing by elicited vaginal or peritoneal PMNs in standard or vaginal conditioned medium (VCM). Results showed that despite significant migration of PMNs and high levels of vaginal beta interleukin-1 (IL-1ß) and alarmin S100A8, CVVC-S mice failed to reduce vaginal fungal burden irrespective of morphology or whether PMNs were present pre- or postinoculation, and had high LDH levels. In contrast, CVVC-R mice had reduced fungal burden and low LDH levels following PMN recruitment and IL-1ß/S100A8 production, but maintained colonization in the absence of PMNs. Elicited vaginal and peritoneal PMNs showed substantial killing activity in standard media or VCM from CVVC-R mice but not in VCM from CVVC-S mice. The inhibitory effect of VCM from CVVC-S mice was unaffected by endogenous or exogenous estrogen and was ablated following depletion/neutralization of Mac-1 ligands using Mac-1 PMNs or recombinant Mac-1. Heparan sulfate (HS) was identified as the putative inhibitor as evidenced by the rescue of PMN killing following heparanase treatment of VCM, as well as by inhibition of killing by purified HS. These results suggest that vaginal HS is linked to PMN dysfunction in CVVC-S mice as a competitive ligand for Mac-1. Vaginal candidiasis, caused by , affects a significant number of women worldwide. Despite an acute inflammatory response by neutrophils during infection, the response fails to reduce the organism. Instead, the response is considered a key process underlying the symptoms of vaginitis. Therefore, it is important to determine the mechanism(s) associated with the lack of vaginal neutrophil antifungal activity. The established mouse model of vaginitis was used to uncover the mechanism of neutrophil dysfunction. Results revealed that heparan sulfate present in the vagina of mice susceptible to chronic vaginitis served as a competitive ligand for the receptor (Mac-1) necessary for fungal recognition and neutrophil-mediated killing. This inhibitory function of heparan sulfate, confirmed through several approaches, provides the first evidence to explain the lack of antifungal immune reactivity during vaginal candidiasis. This finding paves the way for design of therapeutic strategies to reduce/eliminate symptomatic vaginal candidiasis and restore quality of life to those affected.
[Mh] Termos MeSH primário: Candida albicans/imunologia
Candidíase Vulvovaginal/patologia
Heparitina Sulfato/metabolismo
Imunossupressores/metabolismo
Inflamação/patologia
Neutrófilos/efeitos dos fármacos
Neutrófilos/imunologia
[Mh] Termos MeSH secundário: Animais
Candida albicans/crescimento & desenvolvimento
Modelos Animais de Doenças
Feminino
Camundongos
Camundongos Endogâmicos C3H
Camundongos Endogâmicos C57BL
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunosuppressive Agents); 9050-30-0 (Heparitin Sulfate)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE


  7 / 3115 MEDLINE  
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[PMID]:28255766
[Au] Autor:Holzer I; Farr A; Kiss H; Hagmann M; Petricevic L
[Ad] Endereço:Division of Obstetrics and Fetomaternal Medicine, Department of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, Vienna, 1090, Austria.
[Ti] Título:The colonization with Candida species is more harmful in the second trimester of pregnancy.
[So] Source:Arch Gynecol Obstet;295(4):891-895, 2017 Apr.
[Is] ISSN:1432-0711
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Vaginal colonization with Candida species (spp.) during pregnancy has been associated with impaired pregnancy outcomes. There is a reduction in spontaneous preterm birth among women with recurrent asymptomatic colonization of Candida who were treated with clotrimazole. This study aimed to evaluate the impact of the trimester of vulvovaginal colonization with Candida species. METHODS: Data from all women, who were tested positive for the vaginal colonization with Candida spp. during the first or second trimester of pregnancy, and who registered for a planned birth at our tertiary referral center between 2005 and 2014 were retrospectively analyzed. Their preterm birth rate served as the primary outcome variable. Secondary outcome variables were neonatal birthweight and Apgar score. RESULTS: Overall, 1066 women were eligible for the study. In 673 women (63%), who were diagnosed with Candida spp. during the first trimester of pregnancy, the rate of preterm birth was 10% (N = 64). In 393 women (37%), who were diagnosed with candidosis during the second trimester, the preterm birth rate was 18% (N = 71; p = 0.0002). Neonates of women, who presented with vulvovaginal candidosis during the first trimester, had a mean birthweight of 3243 g, compared to 2989 g in the group with a second trimester colonization (p < 0.0001). CONCLUSION: Women who are colonized with Candida spp. during the second trimester of pregnancy have higher rates of preterm birth and lower neonatal birthweight than those who are colonized during the first trimester of their pregnancy. Screening programs for asymptomatic Candida colonization should take this information into account.
[Mh] Termos MeSH primário: Candidíase Vulvovaginal/complicações
[Mh] Termos MeSH secundário: Adulto
Peso ao Nascer
Candida
Feminino
Seres Humanos
Recém-Nascido
Gravidez
Complicações Infecciosas na Gravidez/microbiologia
Resultado da Gravidez
Primeiro Trimestre da Gravidez
Segundo Trimestre da Gravidez
Nascimento Prematuro/etiologia
Estudos Retrospectivos
Fatores de Risco
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1007/s00404-017-4331-y


  8 / 3115 MEDLINE  
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[PMID]:28242128
[Au] Autor:Tardif KD; Schlaberg R
[Ad] Endereço:ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT. Electronic address: keith.d.tardif@aruplab.com.
[Ti] Título:Development of a real-time PCR assay for the direct detection of Candida species causing Vulvovaginal candidiasis.
[So] Source:Diagn Microbiol Infect Dis;88(1):39-40, 2017 May.
[Is] ISSN:1879-0070
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Identification of Candida species by traditional methods can be time-consuming and have limited analytical sensitivity. We developed a multiplex real-time PCR assay for detection and differentiation of Candida species causing vulvovaginal candidiasis (VVC). Overall, this PCR assay is a powerful diagnostic tool offering superior accuracy, sensitivity, and specificity.
[Mh] Termos MeSH primário: Candida/isolamento & purificação
Candidíase Vulvovaginal/diagnóstico
Candidíase Vulvovaginal/microbiologia
Técnicas de Diagnóstico Molecular/métodos
Reação em Cadeia da Polimerase Multiplex/métodos
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Candida/genética
Feminino
Seres Humanos
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170612
[Lr] Data última revisão:
170612
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE


  9 / 3115 MEDLINE  
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[PMID]:28198008
[Au] Autor:Cymerman RM; Kaplan Hoffmann R; Rouhani Schaffer P; Pomeranz MK
[Ad] Endereço:The Ronald O. Perelman Department of Dermatology, NYU School of Medicine, New York, NY, USA.
[Ti] Título:Vulvar infections: beyond sexually transmitted infections.
[So] Source:Int J Dermatol;56(4):361-369, 2017 Apr.
[Is] ISSN:1365-4632
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The vulva is an under-studied area of the female genitourinary tract which is prone to maceration, overgrowth of organisms, and atypical presentations of common dermatologic conditions. In current practice, dermatologists must recognize and manage vulvar infections and infestations beyond the more commonly recognized sexually transmitted infections. Herein, this article reviews the literature on a selection of under-recognized viral, bacterial, fungal, and parasitic vulvar infections and infestations.
[Mh] Termos MeSH primário: Abscesso/complicações
Úlcera Cutânea/virologia
Tuberculose dos Genitais Femininos/complicações
Doenças da Vulva/microbiologia
Doenças da Vulva/parasitologia
[Mh] Termos MeSH secundário: Abscesso/diagnóstico
Abscesso/terapia
Candidíase Vulvovaginal/diagnóstico
Candidíase Vulvovaginal/tratamento farmacológico
Enterobíase/complicações
Enterobíase/diagnóstico
Enterobíase/terapia
Infecções por Vírus Epstein-Barr/complicações
Infecções por Vírus Epstein-Barr/diagnóstico
Infecções por Vírus Epstein-Barr/tratamento farmacológico
Feminino
Herpes Zoster/complicações
Herpes Zoster/diagnóstico
Herpes Zoster/tratamento farmacológico
Seres Humanos
Úlcera Cutânea/terapia
Tuberculose dos Genitais Femininos/diagnóstico
Tuberculose dos Genitais Femininos/tratamento farmacológico
Doenças da Vulva/diagnóstico
Doenças da Vulva/terapia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170216
[St] Status:MEDLINE
[do] DOI:10.1111/ijd.13464


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[PMID]:28185353
[Au] Autor:Shroff A; Sequeira R; Reddy KVR
[Ad] Endereço:Division of Molecular Immunology and Microbiology (MIM), National Institute for Research in Reproductive Health (NIRRH), Mumbai, India.
[Ti] Título:Human vaginal epithelial cells augment autophagy marker genes in response to Candida albicans infection.
[So] Source:Am J Reprod Immunol;77(4), 2017 Apr.
[Is] ISSN:1600-0897
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:PROBLEM: Autophagy plays an important role in clearance of intracellular pathogens. However, no information is available on its involvement in vaginal infections such as vulvo-vaginal candidiasis (VVC). VVC is intimately associated with the immune status of the human vaginal epithelial cells (VECs). The objective of our study is to decipher if autophagy process is involved during Candida albicans infection of VECs. METHODS OF STUDY: In this study, C. albicans infection system was established using human VEC line (VK2/E6E7). Infection-induced change in the expression of autophagy markers like LC3 and LAMP-1 were analyzed by RT-PCR, q-PCR, Western blot, immunofluorescence and transmission electron microscopy (TEM) studies were carried out to ascertain the localization of autophagosomes. Multiplex ELISA was carried out to determine the cytokine profiles. RESULTS: Analysis of LC3 and LAMP-1 expression at mRNA and protein levels at different time points revealed up-regulation of these markers 6 hours post C. albicans infection. LC3 and LAMP-1 puncti were observed in infected VECs after 12 hours. TEM studies showed C. albicans entrapped in autophagosomes. Cytokines-TNF-α and IL-1ß were up-regulated in culture supernatants of VECs at 12 hours post-infection. CONCLUSION: The results suggest that C. albicans invasion led to the activation of autophagy as a host defense mechanism of VECs.
[Mh] Termos MeSH primário: Autofagia/fisiologia
Candidíase Vulvovaginal/patologia
Células Epiteliais/microbiologia
Vagina/microbiologia
[Mh] Termos MeSH secundário: Biomarcadores/análise
Western Blotting
Linhagem Celular
Ensaio de Imunoadsorção Enzimática
Células Epiteliais/patologia
Feminino
Imunofluorescência
Seres Humanos
Microscopia Eletrônica de Transmissão
Reação em Cadeia da Polimerase
Vagina/citologia
Vagina/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170426
[Lr] Data última revisão:
170426
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1111/aji.12639



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