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  1 / 1525 MEDLINE  
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[PMID]:28748632
[Au] Autor:Martínez-DE LA Puente J; Navarro J; Ferraguti M; Soriguer R; Figuerola J
[Ad] Endereço:Estación Biológica de Doñana, Consejo Superior de Investigaciones Científicas (EBD-CSIC), Seville, Spain.
[Ti] Título:First molecular identification of the vertebrate hosts of Culicoides imicola in Europe and a review of its blood-feeding patterns worldwide: implications for the transmission of bluetongue disease and African horse sickness.
[So] Source:Med Vet Entomol;31(4):333-339, 2017 12.
[Is] ISSN:1365-2915
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Culicoides (Diptera: Ceratopogonidae) are vectors of pathogens that affect wildlife, livestock and, occasionally, humans. Culicoides imicola (Kieffer, 1913) is considered to be the main vector of the pathogens that cause bluetongue disease (BT) and African horse sickness (AHS) in southern Europe. The study of blood-feeding patterns in Culicoides is an essential step towards understanding the epidemiology of these pathogens. Molecular tools that increase the accuracy and sensitivity of traditional methods have been developed to identify the hosts of potential insect vectors. However, to the present group's knowledge, molecular studies that identify the hosts of C. imicola in Europe are lacking. The present study genetically characterizes the barcoding region of C. imicola trapped on farms in southern Spain and identifies its vertebrate hosts in the area. The report also reviews available information on the blood-feeding patterns of C. imicola worldwide. Culicoides imicola from Spain feed on blood of six mammals that include species known to be hosts of the BT and AHS viruses. This study provides evidence of the importance of livestock as sources of bloodmeals for C. imicola and the relevance of this species in the transmission of BT and AHS viruses in Europe.
[Mh] Termos MeSH primário: Doença Equina Africana/transmissão
Bluetongue/transmissão
Ceratopogonidae/fisiologia
Código de Barras de DNA Taxonômico
[Mh] Termos MeSH secundário: Animais
Ceratopogonidae/genética
Complexo IV da Cadeia de Transporte de Elétrons/genética
Comportamento Alimentar
Feminino
Proteínas de Insetos/genética
Análise de Sequência de DNA
Espanha
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Insect Proteins); EC 1.9.3.1 (Electron Transport Complex IV)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1111/mve.12247


  2 / 1525 MEDLINE  
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[PMID]:28429824
[Au] Autor:VAN DER Saag MR; Ward MP; Kirkland PD
[Ad] Endereço:Virology Laboratory, Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW, Australia.
[Ti] Título:Application of an embryonated chicken egg model to assess the vector competence of Australian Culicoides midges for bluetongue viruses.
[So] Source:Med Vet Entomol;31(3):263-271, 2017 Sep.
[Is] ISSN:1365-2915
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Culicoides biting midges (Diptera: Ceratopogonidae) are vectors of a number of globally important arboviruses that affect livestock, including bluetongue virus (BTV), African horse sickness virus and the recently emerged Schmallenberg virus. In this study, a model using embryonated chicken eggs (ECEs) was utilized to undertake vector competence studies of Australian Culicoides spp. for 13 laboratory-adapted or wild-type virus strains of BTV. A total of 7393 Culicoides brevitarsis were reared from bovine dung, and 3364 Culicoides were induced to feed from ECEs infected with different strains of BTV. Of those, 911 (27%) survived the putative extrinsic incubation period of 9-12 days. In some trials, virus was also transmitted onward to uninfected ECEs, completing the transmission cycle. This model does not rely on the use of colonized midges and has the capacity to assess the vector competence of field-collected insects with strains of virus that have not previously been passaged in laboratory culture systems. There is also potential for this model to be used in investigations of the competence of Culicoides spp. for other arboviruses.
[Mh] Termos MeSH primário: Vírus Bluetongue/isolamento & purificação
Bluetongue/transmissão
Ceratopogonidae/virologia
Insetos Vetores/virologia
RNA Viral/análise
[Mh] Termos MeSH secundário: Animais
Bluetongue/virologia
Embrião de Galinha
Feminino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170422
[St] Status:MEDLINE
[do] DOI:10.1111/mve.12231


  3 / 1525 MEDLINE  
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[PMID]:28370147
[Au] Autor:Talavera S; Muñoz-Muñoz F; Verdún M; Pagès N
[Ad] Endereço:Institut de Recerca i Tecnologia (IRTA), Centre de Recerca en Sanitat Animal (CReSA), Universitat Autònoma de Barcelona, Barcelona, Spain.
[Ti] Título:Morphology and DNA barcoding reveal three species in one: description of Culicoides cryptipulicaris sp. nov. and Culicoides quasipulicaris sp. nov. in the subgenus Culicoides.
[So] Source:Med Vet Entomol;31(2):178-191, 2017 Jun.
[Is] ISSN:1365-2915
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Species of the genus Culicoides (Diptera: Ceratopogonidae) are well known for their importance in the field of medical and veterinary entomology. Culicoides spp. transmit a wide variety of pathogens, primarily viruses that affect animals and humans. In Europe, the most economically important disease transmitted by Culicoides is bluetongue (BT). Culicoides spp. have been recently involved as primary vectors for Schmallenberg disease. The taxonomy within the subgenus Culicoides has been historically difficult and reorganizations have been proposed regularly. The subgenus Culicoides includes species that are considered to be potential vectors for BT. High morphological intraspecific variability has been attributed to these species. This highlights the apparent presence of previously undetected cryptic species diversity in the subgenus. In the present study, a detailed morphological and molecular study of specimens belonging to Culicoides pulicaris s.l. and specimens resembling a cross between C. pulicaris and Culicoides punctatus revealed the presence of two new species: Culicoides cryptipulicaris and Culicoides quasipulicaris. Females of C. quasipulicaris and males of both species were morphologically distinguished from C. pulicaris (Linnaeus, 1758), whereas females of C. cryptipulicaris were identified using molecular techniques exclusively.
[Mh] Termos MeSH primário: Ceratopogonidae/classificação
Insetos Vetores/classificação
[Mh] Termos MeSH secundário: Animais
Bluetongue/virologia
Ceratopogonidae/anatomia & histologia
Ceratopogonidae/genética
Código de Barras de DNA Taxonômico/veterinária
Feminino
Insetos Vetores/anatomia & histologia
Insetos Vetores/genética
Masculino
Filogenia
Análise de Sequência de DNA/veterinária
Espanha
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1111/mve.12228


  4 / 1525 MEDLINE  
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[PMID]:28347327
[Au] Autor:White SM; Sanders CJ; Shortall CR; Purse BV
[Ad] Endereço:Centre for Ecology & Hydrology, Benson Lane, Wallingford, Oxfordshire, OX10 8BB, UK. smwhit@ceh.ac.uk.
[Ti] Título:Mechanistic model for predicting the seasonal abundance of Culicoides biting midges and the impacts of insecticide control.
[So] Source:Parasit Vectors;10(1):162, 2017 Mar 27.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Understanding seasonal patterns of abundance of insect vectors is important for optimisation of control strategies of vector-borne diseases. Environmental drivers such as temperature, humidity and photoperiod influence vector abundance, but it is not generally known how these drivers combine to affect seasonal population dynamics. METHODS: In this paper, we derive and analyse a novel mechanistic stage-structured simulation model for Culicoides biting midges-the principle vectors of bluetongue and Schmallenberg viruses which cause mortality and morbidity in livestock and impact trade. We model variable life-history traits as functional forms that are dependent on environmental drivers, including air temperature, soil temperature and photoperiod. The model is fitted to Obsoletus group adult suction-trap data sampled daily at five locations throughout the UK for 2008. RESULTS: The model predicts population dynamics that closely resemble UK field observations, including the characteristic biannual peaks of adult abundance. Using the model, we then investigate the effects of insecticide control, showing that control strategies focussing on the autumn peak of adult midge abundance have the highest impact in terms of population reduction in the autumn and averaged over the year. Conversely, control during the spring peak of adult abundance leads to adverse increases in adult abundance in the autumn peak. CONCLUSIONS: The mechanisms of the biannual peaks of adult abundance, which are important features of midge seasonality in northern Europe and are key determinants of the risk of establishment and spread of midge-borne diseases, have been hypothesised over for many years. Our model suggests that the peaks correspond to two generations per year (bivoltine) are largely determined by pre-adult development. Furthermore, control strategies should focus on reducing the autumn peak since the immature stages are released from density-dependence regulation. We conclude that more extensive modelling of Culicoides biting midge populations in different geographical contexts will help to optimise control strategies and predictions of disease outbreaks.
[Mh] Termos MeSH primário: Ceratopogonidae/fisiologia
Simulação por Computador
Surtos de Doenças/prevenção & controle
Controle de Insetos
Insetos Vetores/fisiologia
Estações do Ano
[Mh] Termos MeSH secundário: Animais
Bluetongue/prevenção & controle
Bluetongue/transmissão
Ceratopogonidae/crescimento & desenvolvimento
Meio Ambiente
Europa (Continente)
Nitrilos
Dinâmica Populacional
Piretrinas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nitriles); 0 (Pyrethrins); 2JTS8R821G (decamethrin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170329
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-017-2097-5


  5 / 1525 MEDLINE  
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[PMID]:28299883
[Au] Autor:Sghaier S; Lorusso A; Portanti O; Marcacci M; Orsini M; Barbria ME; Mahmoud AS; Hammami S; Petrini A; Savini G
[Ad] Endereço:Institut de la Recherche Vétérinaire de Tunisie, Laboratoire de virologie, Univérsité de Tunis El Manar, Tunis, Tunisia.
[Ti] Título:A novel Bluetongue virus serotype 3 strain in Tunisia, November 2016.
[So] Source:Transbound Emerg Dis;64(3):709-715, 2017 Jun.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Since 1998, southern Europe has experienced multiple incursions of different serotypes and topotypes of Bluetongue virus, a vector-borne transmitted virus, the causative agent of Bluetongue (BT), a major disease of ruminants. Some of these incursions originated from northern Africa, likely because of wind-blown dissemination of infected midges. In this report, we describe the detection and whole genome characterization of a novel BTV-3 strain identified in a symptomatic sheep in Tunisia. Sequences were immediately deposited with the GenBank Database under Accession Nos KY432369-KY432378. Alert and preparedness are requested to face the next vector seasons in northern Africa and the potential incursion of this novel strain in southern Europe.
[Mh] Termos MeSH primário: Vírus Bluetongue/classificação
Bluetongue/virologia
[Mh] Termos MeSH secundário: Animais
Bluetongue/epidemiologia
Vírus Bluetongue/genética
Estações do Ano
Sorogrupo
Ovinos
Tunísia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12640


  6 / 1525 MEDLINE  
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[PMID]:28298609
[Au] Autor:Brand SP; Keeling MJ
[Ad] Endereço:Zeeman Institute: SBIDER, University of Warwick, Coventry CV4 7AL, UK s.brand@warwick.ac.uk.
[Ti] Título:The impact of temperature changes on vector-borne disease transmission: midges and bluetongue virus.
[So] Source:J R Soc Interface;14(128), 2017 Mar.
[Is] ISSN:1742-5662
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:It is a long recognized fact that climatic variations, especially temperature, affect the life history of biting insects. This is particularly important when considering vector-borne diseases, especially in temperate regions where climatic fluctuations are large. In general, it has been found that most biological processes occur at a faster rate at higher temperatures, although not all processes change in the same manner. This differential response to temperature, often considered as a trade-off between onward transmission and vector life expectancy, leads to the total transmission potential of an infected vector being maximized at intermediate temperatures. Here we go beyond the concept of a static optimal temperature, and mathematically model how realistic temperature variation impacts transmission dynamics. We use bluetongue virus (BTV), under UK temperatures and transmitted by midges, as a well-studied example where temperature fluctuations play a major role. We first consider an optimal temperature profile that maximizes transmission, and show that this is characterized by a warm day to maximize biting followed by cooler weather to maximize vector life expectancy. This understanding can then be related to recorded representative temperature patterns for England, the UK region which has experienced BTV cases, allowing us to infer historical transmissibility of BTV, as well as using forecasts of climate change to predict future transmissibility. Our results show that when BTV first invaded northern Europe in 2006 the cumulative transmission intensity was higher than any point in the last 50 years, although with climate change such high risks are the expected norm by 2050. Such predictions would indicate that regular BTV epizootics should be expected in the UK in the future.
[Mh] Termos MeSH primário: Vírus Bluetongue/fisiologia
Bluetongue
Ceratopogonidae/fisiologia
Mudança Climática
Insetos Vetores/fisiologia
Modelos Biológicos
[Mh] Termos MeSH secundário: Animais
Bluetongue/epidemiologia
Bluetongue/transmissão
Ovinos
Temperatura Ambiente
Reino Unido
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE


  7 / 1525 MEDLINE  
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[PMID]:28237239
[Au] Autor:Gao X; Qin H; Xiao J; Wang H
[Ad] Endereço:Department of Veterinary Surgery, Northeast Agricultural University, Harbin, Heilongjiang, PR China.
[Ti] Título:Meteorological conditions and land cover as predictors for the prevalence of Bluetongue virus in the Inner Mongolia Autonomous Region of Mainland China.
[So] Source:Prev Vet Med;138:88-93, 2017 Mar 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bluetongue is a major disease of economic importance that affects ruminants worldwide. It is transmitted by species of Culicoides midges (Diptera: Ceratopogonidae). The Inner Mongolia Autonomous Region is one of the main pastoral areas for farmed sheep in Mainland China and, because of its large area, represents an ideal candidate region for the study of Bluetongue virus (BTV) distribution and prevalence characteristics. The present study conducted a detailed investigation into the spatial patterns of BTV transmission in sheep in the Inner Mongolia Autonomous Region, and assessed the inter-relationships between meteorological factors, land cover and the transmission of the virus was conducted. Reverse-transcriptase polymerase chain reaction (RT-PCR) was used for the determination of BTV infection in the surveyed animals. Between June 2013 and February 2015, 6199 sheep were subjected to virus detection and 2199 sheep (35.47%) were determined to be positive for BTV. Subsequently, a maximum entropy model (MaxEnt) was used to investigate the relationship between land cover, meteorological factors and the prevalence of BTV infection. Jackknife analysis revealed that the mean monthly temperature, rainfall and average wind speed were associated with the occurrence of BTV infection and that BTV infection positivity was significantly higher among animals from districts with a high percentage of grassland and forest area. Our findings indicate that meteorological factors and land cover may be important variables affecting transmission of BTV and should be taken into account in the development of future surveillance programmes for BTV.
[Mh] Termos MeSH primário: Bluetongue/epidemiologia
Bluetongue/transmissão
Conceitos Meteorológicos
[Mh] Termos MeSH secundário: Animais
Bluetongue/sangue
Ceratopogonidae/virologia
China/epidemiologia
Insetos Vetores/virologia
Mapas como Assunto
Prevalência
Curva ROC
Chuvas
Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária
Fatores de Risco
Ovinos
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170227
[St] Status:MEDLINE


  8 / 1525 MEDLINE  
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[PMID]:28222780
[Au] Autor:Vidic J; Manzano M; Chang CM; Jaffrezic-Renault N
[Ad] Endereço:Virologie et Immunologie Moléculaires, UR892, INRA, Paris Saclay University, 78350, Jouy-en-Josas, France. jasmina.vidic@inra.fr.
[Ti] Título:Advanced biosensors for detection of pathogens related to livestock and poultry.
[So] Source:Vet Res;48(1):11, 2017 Feb 21.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Infectious animal diseases caused by pathogenic microorganisms such as bacteria and viruses threaten the health and well-being of wildlife, livestock, and human populations, limit productivity and increase significantly economic losses to each sector. The pathogen detection is an important step for the diagnostics, successful treatment of animal infection diseases and control management in farms and field conditions. Current techniques employed to diagnose pathogens in livestock and poultry include classical plate-based methods and conventional biochemical methods as enzyme-linked immunosorbent assays (ELISA). These methods are time-consuming and frequently incapable to distinguish between low and highly pathogenic strains. Molecular techniques such as polymerase chain reaction (PCR) and real time PCR (RT-PCR) have also been proposed to be used to diagnose and identify relevant infectious disease in animals. However these DNA-based methodologies need isolated genetic materials and sophisticated instruments, being not suitable for in field analysis. Consequently, there is strong interest for developing new swift point-of-care biosensing systems for early detection of animal diseases with high sensitivity and specificity. In this review, we provide an overview of the innovative biosensing systems that can be applied for livestock pathogen detection. Different sensing strategies based on DNA receptors, glycan, aptamers and antibodies are presented. Besides devices still at development level some are validated according to standards of the World Organization for Animal Health and are commercially available. Especially, paper-based platforms proposed as an affordable, rapid and easy to perform sensing systems for implementation in field condition are included in this review.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/veterinária
Influenza Aviária/diagnóstico
Gado/microbiologia
Gado/virologia
Doenças das Aves Domésticas/diagnóstico
[Mh] Termos MeSH secundário: Animais
Técnicas Biossensoriais/métodos
Bluetongue/diagnóstico
Complexo Respiratório Bovino/diagnóstico
Infecções por Campylobacter/diagnóstico
Infecções por Campylobacter/veterinária
Bovinos
Galinhas/microbiologia
Galinhas/virologia
Infecções por Clostridium/diagnóstico
Infecções por Clostridium/veterinária
Coccidiose/diagnóstico
Coccidiose/virologia
Infecções por Escherichia coli/diagnóstico
Infecções por Escherichia coli/veterinária
Feminino
Febre Aftosa/diagnóstico
Mastite Bovina/diagnóstico
Infecções por Mycoplasma/diagnóstico
Infecções por Mycoplasma/veterinária
Infecções por Orthomyxoviridae/diagnóstico
Infecções por Orthomyxoviridae/veterinária
Salmonelose Animal/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1186/s13567-017-0418-5


  9 / 1525 MEDLINE  
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[PMID]:28193493
[Au] Autor:Ten Haaf A; Kohl J; Pscherer S; Hamann HP; Eskens HU; Bastian M; Gattenlöhner S; Tur MK
[Ad] Endereço:Institute of Pathology, University Hospital Giessen, Justus-Liebig-University Giessen, Langhans-Str. 10, 35392 Giessen, Germany. Electronic address: Andre.T.Haaf@patho.med.uni-giessen.de.
[Ti] Título:Development of a monoclonal sandwich ELISA for direct detection of bluetongue virus 8 in infected animals.
[So] Source:J Virol Methods;243:172-176, 2017 May.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bluetongue is an infectious viral disease which can cause mortality in affected ruminants, and tremendous economic damage via impacts upon fertility, milk production and the quality of wool. The disease is caused by bluetongue virus (BTV) which is transmitted by species of Culicoides biting midge. Rapid detection of BTV is required to contain disease outbreaks and reduce economic losses. The purpose of this study was to develop a monoclonal sandwich ELISA for direct detection of BTV in infected animals. Phage display technology was used to isolate BTV specific antibody fragments by applying the human scFv Tomlinson antibody libraries directly on purified BTV-8 particles. Three unique BTV-8 specific human antibody fragments were isolated which were able to detect purified BTV particles and also BTV in serum of an infected sheep. A combination of a human/mouse scFv-Fc chimeric fusion protein and a human Fab fragment in a sandwich ELISA format was able to detect BTV specifically with a limit of detection (LOD) of 10 infectious virus particles, as determined by tissue culture titration. This approach provided pilot data towards the development of a novel diagnostic test that might be used for direct detection of BTV-8 particles.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/imunologia
Anticorpos Antivirais/imunologia
Antígenos Virais/análise
Vírus Bluetongue/isolamento & purificação
Bluetongue/diagnóstico
Ensaio de Imunoadsorção Enzimática/métodos
Soro/virologia
[Mh] Termos MeSH secundário: Animais
Bluetongue/virologia
Vírus Bluetongue/imunologia
Camundongos
Testes Sorológicos/métodos
Ovinos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Viral); 0 (Antigens, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE


  10 / 1525 MEDLINE  
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[PMID]:28132926
[Au] Autor:Du J; Gao S; Tian Z; Xing S; Huang D; Zhang G; Zheng Y; Liu G; Luo J; Chang H; Yin H
[Ad] Endereço:State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu 730046, PR China. Electronic address: dujunzheng@caas.cn.
[Ti] Título:MicroRNA expression profiling of primary sheep testicular cells in response to bluetongue virus infection.
[So] Source:Infect Genet Evol;49:256-267, 2017 Apr.
[Is] ISSN:1567-7257
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Bluetongue virus (BTV) is a member of the genus Orbivirus within the family Reoviridae and causes a non-contagious, insect-transmitted disease in domestic and wild ruminants, mainly in sheep and occasionally in cattle and some species of deer. Virus infection can trigger the changes of the cellular microRNA (miRNA) expression profile, which play important post-transcriptional regulatory roles in gene expression and can greatly influence viral replication and pathogenesis. Here, we employed deep sequencing technology to determine which cellular miRNAs were differentially expressed in primary sheep testicular (ST) cells infected with BTV. A total of 25 known miRNAs and 240 novel miRNA candidates that were differentially expressed in BTV-infected and uninfected ST cells were identified, and 251 and 8428 predicted target genes were annotated, respectively. Nine differentially expressed miRNAs and their mRNA targets were validated by quantitative reverse transcription-polymerase chain reaction. Targets prediction and functional analysis of these regulated miRNAs revealed significant enrichment for several signaling pathways including MAPK, PI3K-Akt, endocytosis, Hippo, NF-kB, viral carcinogenesis, FoxO, and JAK-STAT signaling pathways. This study provides a valuable basis for further investigation on the roles of miRNAs in BTV replication and pathogenesis.
[Mh] Termos MeSH primário: Vírus Bluetongue/crescimento & desenvolvimento
Bluetongue/genética
Regulação da Expressão Gênica
Interações Hospedeiro-Patógeno
MicroRNAs/genética
Testículo/metabolismo
[Mh] Termos MeSH secundário: Animais
Bluetongue/metabolismo
Bluetongue/patologia
Bluetongue/virologia
Vírus Bluetongue/patogenicidade
Fatores de Transcrição Forkhead/genética
Fatores de Transcrição Forkhead/metabolismo
Perfilação da Expressão Gênica
Ontologia Genética
Sequenciamento de Nucleotídeos em Larga Escala
Janus Quinases/genética
Janus Quinases/metabolismo
Masculino
MicroRNAs/metabolismo
Proteínas Quinases Ativadas por Mitógeno/genética
Proteínas Quinases Ativadas por Mitógeno/metabolismo
Anotação de Sequência Molecular
Fosfatidilinositol 3-Quinases/genética
Fosfatidilinositol 3-Quinases/metabolismo
Cultura Primária de Células
Proteínas Proto-Oncogênicas c-akt/genética
Proteínas Proto-Oncogênicas c-akt/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Fatores de Transcrição STAT/genética
Fatores de Transcrição STAT/metabolismo
Carneiro Doméstico
Transdução de Sinais
Testículo/patologia
Testículo/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Forkhead Transcription Factors); 0 (MicroRNAs); 0 (STAT Transcription Factors); EC 2.7.1.- (Phosphatidylinositol 3-Kinases); EC 2.7.10.2 (Janus Kinases); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170131
[St] Status:MEDLINE



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