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[PMID]:28467177
[Au] Autor:Sayler KA; Bigelow T; Koster LG; Swenson S; Bounds C; Hernández F; Wisely SM
[Ad] Endereço:Department of Wildlife Ecology and Conservation, University of Florida, Gainesville, FL (Sayler, Bounds, Hernández, Wisely).
[Ti] Título:Development of a rapid, simple, and specific real-time PCR assay for detection of pseudorabies viral DNA in domestic swine herds.
[So] Source:J Vet Diagn Invest;29(4):522-528, 2017 Jul.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Despite successful eradication of pseudorabies virus (PRV) from the commercial pig industry in the United States in 2004, large populations of feral swine in certain regions act as wildlife reservoirs for the virus. Given the threat of reintroduction of the virus into domestic herds, a rapid, reliable, easily implemented assay is needed for detection of PRV. Although a real-time PCR (rtPCR) assay exists, improvements in rtPCR technology and a greater understanding of the diversity of PRV strains worldwide require an assay that would be easier to implement, more cost effective, and more specific. We developed a single-tube, rapid rtPCR that is capable of detecting 10 copies of PRV glycoprotein B ( gB) DNA per 20-µL total volume reaction. The assay did not produce a false-positive in samples known to be negative for the virus. The assay was negative for genetically similar herpesviruses and other porcine viruses. Our assay is a highly specific and sensitive assay that is also highly repeatable and reproducible. The assay should be a useful tool for early detection of PRV in pigs in the case of a suspected introduction or outbreak situation.
[Mh] Termos MeSH primário: DNA Viral/análise
Herpesvirus Suídeo 1/isolamento & purificação
Pseudorraiva/diagnóstico
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Doenças dos Suínos/diagnóstico
Proteínas do Envelope Viral/análise
[Mh] Termos MeSH secundário: Animais
Pseudorraiva/virologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
Sensibilidade e Especificidade
Sus scrofa
Suínos
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Viral Envelope Proteins); 0 (glycoprotein gII, pseudorabies virus)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171212
[Lr] Data última revisão:
171212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1177/1040638717706593


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[PMID]:28363130
[Au] Autor:Wang X; Wu CX; Song XR; Chen HC; Liu ZF
[Ad] Endereço:State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
[Ti] Título:Comparison of pseudorabies virus China reference strain with emerging variants reveals independent virus evolution within specific geographic regions.
[So] Source:Virology;506:92-98, 2017 Jun.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pseudorabies virus (PRV) China reference strain Ea is genetically closely related to newly emerged variants; however, there is limited information about PRV Ea. Here, we compared PRV Ea with new variant strains by growth kinetics, genome sequencing, and protein expression analysis. Growth analysis showed that strain Ea forms smaller plaques than strain HNX. The full-length genome sequence of Ea revealed that it is clustered in the same subgroup as HNX. Ea and HNX strains exhibited similar extracellular virion protein polymorphisms, whereas strain Bartha expressed less VP26 and more GAPDH. In infected cells, strain Ea expressed high levels of IE180 protein, and Ea and HNX produced higher levels of UL21 protein than strain Bartha. These findings provide evidence that PRV China reference strain Ea is genetically closely related to the newly emerged variant strains, indicating that strain PRV China may have evolved independently leading to the emergence of a variant strain.
[Mh] Termos MeSH primário: Evolução Molecular
Herpesvirus Suídeo 1/genética
Herpesvirus Suídeo 1/isolamento & purificação
Pseudorraiva/virologia
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
China
Herpesvirus Suídeo 1/classificação
Herpesvirus Suídeo 1/metabolismo
Filogenia
Suínos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170705
[Lr] Data última revisão:
170705
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170401
[St] Status:MEDLINE


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[PMID]:28283558
[Au] Autor:Pomeranz LE; Ekstrand MI; Latcha KN; Smith GA; Enquist LW; Friedman JM
[Ad] Endereço:Laboratory of Molecular Genetics, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10065.
[Ti] Título:Gene Expression Profiling with Cre-Conditional Pseudorabies Virus Reveals a Subset of Midbrain Neurons That Participate in Reward Circuitry.
[So] Source:J Neurosci;37(15):4128-4144, 2017 Apr 12.
[Is] ISSN:1529-2401
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The mesolimbic dopamine pathway receives inputs from numerous regions of the brain as part of a neural system that detects rewarding stimuli and coordinates a behavioral response. The capacity to simultaneously map and molecularly define the components of this complex multisynaptic circuit would thus advance our understanding of the determinants of motivated behavior. To accomplish this, we have constructed pseudorabies virus (PRV) strains in which viral propagation and fluorophore expression are activated only after exposure to Cre recombinase. Once activated in Cre-expressing neurons, the virus serially labels chains of presynaptic neurons. Dual injection of GFP and mCherry tracing viruses simultaneously illuminates nigrostriatal and mesolimbic circuitry and shows no overlap, demonstrating that PRV transmission is confined to synaptically connected neurons. To molecularly profile mesolimbic dopamine neurons and their presynaptic inputs, we injected Cre-conditional GFP virus into the NAc of (anti-GFP) nanobody-L10 transgenic mice and immunoprecipitated translating ribosomes from neurons infected after retrograde tracing. Analysis of purified RNA revealed an enrichment of transcripts expressed in neurons of the dorsal raphe nuclei and lateral hypothalamus that project to the mesolimbic dopamine circuit. These studies identify important inputs to the mesolimbic dopamine pathway and further show that PRV circuit-directed translating ribosome affinity purification can be broadly applied to identify molecularly defined neurons comprising complex, multisynaptic circuits. The mesolimbic dopamine circuit integrates signals from key brain regions to detect and respond to rewarding stimuli. To further define this complex multisynaptic circuit, we constructed a panel of Cre recombinase-activated pseudorabies viruses (PRVs) that enabled retrograde tracing of neural inputs that terminate on Cre-expressing neurons. Using these viruses and Retro-TRAP (translating ribosome affinity purification), a previously reported molecular profiling method, we developed a novel technique that provides anatomic as well as molecular information about the neural components of polysynaptic circuits. We refer to this new method as PRV-Circuit-TRAP (PRV circuit-directed TRAP). Using it, we have identified major projections to the mesolimbic dopamine circuit from the lateral hypothalamus and dorsal raphe nucleus and defined a discrete subset of transcripts expressed in these projecting neurons, which will allow further characterization of this important pathway. Moreover, the method we report is general and can be applied to the study of other neural circuits.
[Mh] Termos MeSH primário: Perfilação da Expressão Gênica/métodos
Integrases/análise
Mesencéfalo/química
Neurônios/química
Pseudorraiva
Recompensa
[Mh] Termos MeSH secundário: Animais
Feminino
Integrases/metabolismo
Masculino
Mesencéfalo/anatomia & histologia
Mesencéfalo/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Neurônios/metabolismo
Pseudorraiva/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.7.7.- (Cre recombinase); EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170312
[St] Status:MEDLINE
[do] DOI:10.1523/JNEUROSCI.3193-16.2017


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[PMID]:28214681
[Au] Autor:Liang C; Tong W; Zheng H; Liu F; Wu J; Li G; Zhou EM; Tong G
[Ad] Endereço:College of Veterinary Medicine, Northwest A & F University, Yangling 712100, Shaanxi, China; Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
[Ti] Título:A high-temperature passaging attenuated Pseudorabies vaccine protects piglets completely against emerging PRV variant.
[So] Source:Res Vet Sci;112:109-115, 2017 Jun.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Emerging variant of pseudorabies virus (PRV) have evaded the antiviral immunity of commercially available PRV vaccine and have led to PRV outbreaks in Chinese pig farms. Here, we attenuated a PRV variant strain by serial passages in vitro and evaluate the protective efficacy of the attenuated strain as a vaccine candidate. The virulent PRV variant strain JS-2012 was continuously passaged in Vero cells at 40°C and attenuated rapidly. After 90 passages in Vero cells, the passaged virus lost its ability to cause death in 2-week-old piglets. The 120th passage virus was avirulent in the sucking piglets. An attenuated strain, JS-2012-F120 derived from the 120th passage virus by three rounds of plaque cloning grew better than its parent strain JS-2012 in Vero cells and showed notably different cytopathic effects and plaque morphology from JS-2012. PCR combined with sequence analysis showed that JS-2012-F120 contained a 2307-bp deletion covering nucleotide 487 of gE gene to 531 of US2 gene. After inoculation with JS-2012-F120, young piglets were completely protected from challenge with the classical and emerging virulent PRVs. Moreover, the piglets did not develop specific gE antibodies. Thus, JS-2012-F120 appears to be a promising marker vaccine to control PRV variant circulating in Chinese pig farms, and the high-temperature passaging in vitro was an efficient method to attenuated alphaherpesvirus.
[Mh] Termos MeSH primário: Herpesvirus Suídeo 1/genética
Vacinas contra Pseudorraiva/imunologia
Pseudorraiva/prevenção & controle
Doenças dos Suínos/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Surtos de Doenças
Suínos
Doenças dos Suínos/virologia
Temperatura Ambiente
Vacinação
Vacinas Atenuadas/imunologia
Proteínas do Envelope Viral/genética
[Pt] Tipo de publicação:CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Pseudorabies Vaccines); 0 (Vaccines, Attenuated); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170220
[St] Status:MEDLINE


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[PMID]:28202055
[Au] Autor:Sonnenburg J; Ryser-Degiorgis MP; Kuiken T; Ferroglio E; Ulrich RG; Conraths FJ; Gortázar C; Staubach C; APHAEA project partners
[Ad] Endereço:Federal Research Institute for Animal Health, Friedrich-Loeffler-Institut, Institute of Epidemiology, Südufer 10, 17493, Greifswald, Insel Riems, Germany. janson@vet.dtu.dk.
[Ti] Título:Harmonizing methods for wildlife abundance estimation and pathogen detection in Europe-a questionnaire survey on three selected host-pathogen combinations.
[So] Source:BMC Vet Res;13(1):53, 2017 Feb 16.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The need for wildlife health surveillance as part of disease control in wildlife, domestic animals and humans on the global level is widely recognized. However, the objectives, methods and intensity of existing wildlife health surveillance programs vary greatly among European countries, resulting in a patchwork of data that are difficult to merge and compare. This survey aimed at evaluating the need and potential for data harmonization in wildlife health in Europe. The specific objective was to collect information on methods currently used to estimate host abundance and pathogen prevalence. Questionnaires were designed to gather detailed information for three host-pathogen combinations: (1) wild boar and Aujeszky's disease virus, (2) red fox and Echinococcus multilocularis, and (3) common vole and Francisella tularensis. RESULTS: We received a total of 70 responses from 19 European countries. Regarding host abundance, hunting bags are currently the most widely accessible data source for widely distributed mid-sized and larger mammals such as red fox and wild boar, but we observed large differences in hunting strategies among countries as well as among different regions within countries. For small rodents, trapping is the method of choice, but practical applications vary among study sites. Laboratory procedures are already largely harmonized but information on the sampled animals is not systematically collected. CONCLUSIONS: The answers revealed that a large amount of information is available for the selected host-pathogen pairs and that in theory methods are already largely harmonized. However, the comparability of the data remains strongly compromised by local differences in the way, the methods are applied in practice. While these issues may easily be overcome for prevalence estimation, there is an urgent need to develop tools for the routine collection of host abundance data in a harmonized way. Wildlife health experts are encouraged to apply the harmonized APHAEA protocols in epidemiological studies in wildlife and to increase cooperation.
[Mh] Termos MeSH primário: Arvicolinae/microbiologia
Equinococose/veterinária
Echinococcus multilocularis/isolamento & purificação
Raposas/parasitologia
Pseudorraiva/virologia
Tularemia/veterinária
[Mh] Termos MeSH secundário: Animais
Equinococose/parasitologia
Europa (Continente)/epidemiologia
Francisella tularensis/isolamento & purificação
Herpesvirus Suídeo 1/isolamento & purificação
Densidade Demográfica
Pseudorraiva/epidemiologia
Inquéritos e Questionários
Sus scrofa
Suínos
Doenças dos Suínos/epidemiologia
Doenças dos Suínos/virologia
Tularemia/epidemiologia
Tularemia/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-016-0935-x


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[PMID]:28122975
[Au] Autor:Lamote JA; Kestens M; Van Waesberghe C; Delva J; De Pelsmaeker S; Devriendt B; Favoreel HW
[Ad] Endereço:Department of Virology, Parasitology, and Immunology, Faculty of Veterinary Medicine, Ghent University, Belgium.
[Ti] Título:The Pseudorabies Virus Glycoprotein gE/gI Complex Suppresses Type I Interferon Production by Plasmacytoid Dendritic Cells.
[So] Source:J Virol;91(7), 2017 Apr 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plasmacytoid dendritic cells (pDC) play a central role in the antiviral immune response, both in the innate response and in shaping the adaptive response, mainly because of their ability to produce massive amounts of type I interferon (TI-IFN). Here, we report that cells infected with the live attenuated Bartha vaccine strain of porcine alphaherpesvirus pseudorabies virus (PRV) trigger a dramatically increased TI-IFN response by porcine primary pDC compared to cells infected with wild-type PRV strains (Becker and Kaplan). Since Bartha is one of the relatively few examples of a highly successful alphaherpesvirus vaccine, identification of factors that may contribute to its efficacy may provide insights for the rational design of other alphaherpesvirus vaccines. The Bartha vaccine genome displays several mutations compared to the genome of wild-type PRV strains, including a large deletion in the unique short (US) region, encompassing the glycoprotein E (gE), gI, US9, and US2 genes. Using recombinant PRV Becker strains harboring the entire Bartha US deletion or single mutations in the four affected US genes, we demonstrate that the absence of the viral gE/gI complex contributes to the observed increased IFN-α response. Furthermore, we show that the absence of gE leads to an enhanced extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation in pDC, which correlates with a higher TI-IFN production by pDC. In conclusion, the PRV Bartha vaccine strain triggers strongly increased TI-IFN production by porcine pDC. Our data further indicate that the gE/gI glycoprotein complex suppresses TI-IFN production by pDC, which represents the first alphaherpesvirus factor that suppresses pDC activity. Several alphaherpesviruses, including herpes simpex virus, still lack effective vaccines. However, the highly successful Bartha vaccine has contributed substantially to eradication of the porcine alphaherpesvirus pseudorabies virus (PRV) in several countries. The impact of Bartha on the immune response is still poorly understood. Type I interferon (TI-IFN)-producing plasmacytoid dendritic cells (pDC) may play an important role in vaccine development. Here, we show that Bartha elicits a dramatically increased type I interferon (TI-IFN) response in primary porcine pDC compared to wild-type strains. In addition, we found that the gE/gI complex, which is absent in Bartha, inhibits the pDC TI-IFN response. This is the first description of an immune cell type that is differentially affected by Bartha versus wild-type PRV and is the first report describing an alphaherpesvirus protein that inhibits the TI-IFN response by pDC. These data may therefore contribute to the rational design of other alphaherpesvirus vaccines.
[Mh] Termos MeSH primário: Células Dendríticas/metabolismo
Interferon Tipo I/biossíntese
Pseudorraiva/imunologia
Proteínas do Envelope Viral/fisiologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Células Dendríticas/imunologia
Células Dendríticas/virologia
Evasão da Resposta Imune
Imunidade Inata
Imunomodulação
Sistema de Sinalização das MAP Quinases
Pseudorraiva/virologia
Sus scrofa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interferon Type I); 0 (Viral Envelope Proteins); 0 (glycoprotein E, Suid herpesvirus 1)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE


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[PMID]:28062697
[Au] Autor:Rowe AM; Yun H; Treat BR; Kinchington PR; Hendricks RL
[Ad] Endereço:Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15201; alr124@pitt.edu.
[Ti] Título:Subclinical Herpes Simplex Virus Type 1 Infections Provide Site-Specific Resistance to an Unrelated Pathogen.
[So] Source:J Immunol;198(4):1706-1717, 2017 Feb 15.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:HSV-1 infections of the cornea range in severity from minor transient discomfort to the blinding disease herpes stromal keratitis, yet most patients experience a single episode of epithelial keratitis followed by re-establishment of a clear cornea. We asked whether a single transient episode of HSV-1 epithelial keratitis causes long-term changes in the corneal microenvironment that influence immune responses to subsequent corneal infection or trauma. We showed that C57BL/6 mouse corneas infected with HSV-1 KOS, which induces transient herpes epithelial keratitis without herpes stromal keratitis sequelae, possessed a significant leukocytic infiltrate composed primarily of CD4 T cells and macrophages along with elevated chemokines and cytokines that persisted without loss of corneal clarity (subclinical inflammation). Chemokine and cytokine expression was CD4 T cell dependent, in that their production was significantly reduced by systemic CD4 T cell depletion starting before infection, although short-term (3-d) local CD4 T cell depletion postinfection did not influence chemokine levels in cornea. Corneas with subclinical inflammation developed significantly greater trauma-induced inflammation when they were recipients of syngeneic corneal transplants but also exhibited significantly increased resistance to infections by unrelated pathogens, such as pseudorabies virus. The resistance to pseudorabies virus was CD4 T cell dependent, because it was eliminated by local CD4 T cell depletion from the cornea. We conclude that transient HSV-1 corneal infections cause long-term alterations of the corneal microenvironment that provide CD4-dependent innate resistance to subsequent infections by antigenically unrelated pathogens.
[Mh] Termos MeSH primário: Infecções Assintomáticas
Linfócitos T CD4-Positivos/imunologia
Córnea/imunologia
Herpes Simples/imunologia
Herpesvirus Humano 1/imunologia
Herpesvirus Suídeo 1/patogenicidade
Ceratite Herpética/imunologia
Pseudorraiva/imunologia
[Mh] Termos MeSH secundário: Animais
Quimiocinas/biossíntese
Quimiocinas/imunologia
Córnea/patologia
Córnea/virologia
Transplante de Córnea
Citocinas/biossíntese
Citocinas/imunologia
Feminino
Herpes Simples/virologia
Herpesvirus Suídeo 1/imunologia
Imunidade Inata
Inflamação/imunologia
Inflamação/virologia
Ceratite Herpética/fisiopatologia
Ceratite Herpética/virologia
Macrófagos/imunologia
Camundongos
Camundongos Endogâmicos C57BL
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chemokines); 0 (Cytokines)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170108
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601310


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[PMID]:28056966
[Au] Autor:Masot AJ; Gil M; Risco D; Jiménez OM; Núñez JI; Redondo E
[Ad] Endereço:Unidad de Histología y Anatomía Patológica, Departamento de Medicina Animal, Facultad de Veterinaria, Universidad de Extremadura, Avda. Universidad s.n., Cáceres, 10003, Spain. jahis@unex.es.
[Ti] Título:Pseudorabies virus infection (Aujeszky's disease) in an Iberian lynx (Lynx pardinus) in Spain: a case report.
[So] Source:BMC Vet Res;13(1):6, 2017 Jan 05.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The only natural hosts of Pseudorabies virus (PRV) are members of the family Suidae (Sus scrofa scrofa). In species other than suids infection is normally fatal. In these mammals, including carnivores, PRV typically causes serious neurologic disease. The endangered Iberian lynx (Lynx pardinus) is a wild feline endemic to south-western Europe (Iberian Peninsula). The Iberian lynx was found to be the world's most endangered felid species in 2002. In wild felines, PRV infection has only been previously reported once in a Florida panther in 1994. No seropositive lynxes have ever been found, nor has PRV been detected in dead Iberian lynxes to date. CASE PRESENTATION: We describe the first reported case of pseudorabies in an Iberian lynx (Lynx pardinus). Pseudorabies was diagnosed in a young wild Iberian lynx from Extremadura (SW Spain) by histopathological examination, immunohistochemistry, polymerase chain reaction (PCR) and sequence analysis. Gross lesions included alopecia of the ventral neck, bloody gastro-intestinal contents and congestion of the brain. Histopathological analysis showed a moderate nonsuppurative meningoencephalitis with diffuse areas of demyelination, necrotizing gastritis and enteritis of the small intestine. Pseudorabies virus (PRV) antigen was found in neuronal and non-neuronal cells of the brain, tonsils, and gastric glandular epithelial cells by immunohistochemical analysis. The presence of the virus in the brain was confirmed by nested PCR. The sequence analysis of the 146 bp fragment (from the viral glycoprotein B gene) showed that the amplified sequence matched (with 100% identity) the PRV genome. Furthermore, specific DNA from glycoprotein D and E encoding-genes was detected by conventional and real-time PCR, respectively, confirming the latter that this infection was produced by a wild-type PRV strain. CONCLUSIONS: This study supports the suspicion that PRV could infect the Iberian lynx. The detection of PRV in a dead Iberian lynx suggests that the virus may have a negative impact on the survival of endangered lynxes in the wild. However, because this is the first verified instance of lynx mortality resulting from pseudorabies, its true impact on the population is unknown.
[Mh] Termos MeSH primário: Herpesvirus Suídeo 1/isolamento & purificação
Lynx/virologia
Pseudorraiva/epidemiologia
[Mh] Termos MeSH secundário: Animais
Evolução Fatal
Masculino
Espanha/epidemiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-016-0938-7


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[PMID]:27894861
[Au] Autor:Vrublevskaya VV; Afanasyev VN; Grinevich AA; Skarga YY; Gladyshev PP; Ibragimova SA; Krylsky DV; Morenkov OS
[Ad] Endereço:Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142290 Russia.
[Ti] Título:Development of a competitive double antibody lateral flow assay for the detection of antibodies specific to glycoprotein B of Aujeszky's disease virus in swine sera.
[So] Source:J Virol Methods;240:54-62, 2017 Feb.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Three lateral flow assays (LFAs) for the detection of antibodies against glycoprotein B (gB) of Aujeszky's disease virus (ADV) in swine sera: a competitive double antibody sandwich LFA without a preincubation step (CDAS-gB-LFA), a CDAS-gB-LFA with a preincubation step (pCDAS-gB-LFA), and a competitive direct gB-LFA have been developed and were compared with each other and with a gB-ELISA. The assays are based on monoclonal antibodies to immunodominant epitopes of ADV gB. The pCDAS-gB-LFA proved to be the most specific and sensitive assay to detect antibodies directed to ADV gB. The specificity and sensitivity of the pCDAS-gB-LFA with the use of an LFA reader for test line intensity measurements were 97.6 and 94.9%, respectively. The lower diagnostic sensitivity of the pCDAS-gB-LFA compared to a gB-ELISA reflects its reduced analytical sensitivity, which was shown in titration experiments with positive sera. The pCDAS-gB-LFA, using the reader-based and visual detection modes, showed good agreement in respect to specificity; however, the LFA reader detection provided a higher diagnostic and analytical sensitivity compared to visual detection. The developed pCDAS-gB-LFA is a rapid, sensitive, and specific method for the detection of antibodies to ADV gB and can be used for screening ADV-infected swine in unvaccinated herds.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/imunologia
Anticorpos Antivirais/sangue
Glicoproteínas/imunologia
Herpesvirus Suídeo 1/imunologia
Pseudorraiva/diagnóstico
Doenças dos Suínos/diagnóstico
Proteínas do Envelope Viral/imunologia
[Mh] Termos MeSH secundário: Animais
Antígenos Virais/imunologia
Ensaio de Imunoadsorção Enzimática
Herpesvirus Suídeo 1/isolamento & purificação
Testes de Neutralização
Pseudorraiva/imunologia
Sensibilidade e Especificidade
Suínos
Doenças dos Suínos/imunologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (Glycoproteins); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161130
[St] Status:MEDLINE


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[PMID]:27885561
[Au] Autor:Liu H; Li XT; Hu B; Deng XY; Zhang L; Lian SZ; Zhang HL; Lv S; Xue XH; Lu RG; Shi N; Yan MH; Xiao PP; Yan XJ
[Ad] Endereço:Division of Infectious Diseases of Special Economic Animals, State Key Laboratory for Molecular Biology of Special Economic Animals, Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130122, China.
[Ti] Título:Outbreak of severe pseudorabies virus infection in pig-offal-fed farmed mink in Liaoning Province, China.
[So] Source:Arch Virol;162(3):863-866, 2017 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:An outbreak of severe pseudorabies virus (PRV) infection in farmed mink occurred in northern China in late 2014, causing significant economic losses in the local fur industry. Here, we report the first case of a PRV outbreak in mink in northeastern China, caused by feeding farmed mink with raw pork or organs contaminated by PRV. Mink infected with virulent PRV exhibited diarrhea, neurologic signs, and higher mortality, which can be misdiagnosed as highly pathogenic mink enteritis virus (MEV), canine distemper virus (CDV), and food poisoning. However, these were excluded as causative agents by PCR or bacteria isolation. The duration of disease was 3-7 days, and the mortality rate was 80-90%. PRV was characterized using indirect immunofluorescence assays (IFA) and electron microscopy (EM). Phylogenetic analysis based on full-length genome sequences and those of individual genes of this novel virus strain showed that it clustered in an independent branch with several other PRV isolates from China.
[Mh] Termos MeSH primário: Ração Animal/virologia
Herpesvirus Suídeo 1/isolamento & purificação
Vison/virologia
Pseudorraiva/virologia
[Mh] Termos MeSH secundário: Ração Animal/análise
Animais
China/epidemiologia
Contaminação de Alimentos/análise
Herpesvirus Suídeo 1/classificação
Herpesvirus Suídeo 1/genética
Herpesvirus Suídeo 1/fisiologia
Filogenia
Pseudorraiva/epidemiologia
Pseudorraiva/transmissão
Carne Vermelha/virologia
Suínos
Doenças dos Suínos/epidemiologia
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3170-7



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