Base de dados : MEDLINE
Pesquisa : C02.256 [Categoria DeCS]
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  1 / 1514 MEDLINE  
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[PMID]:29247338
[Au] Autor:Kemenesi G; Kurucz K; Zana B; Földes F; Urbán P; Vlaschenko A; Kravchenko K; Budinski I; Szodoray-Parádi F; Bücs S; Jére C; Csosz I; Szodoray-Parádi A; Estók P; Görföl T; Boldogh S; Jakab F
[Ad] Endereço:Virological Research Group, János Szentágothai Research Centre, University of Pécs, Pécs, Hungary. kemenesi.gabor@gmail.com.
[Ti] Título:Diverse replication-associated protein encoding circular DNA viruses in guano samples of Central-Eastern European bats.
[So] Source:Arch Virol;163(3):671-678, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Circular replication-associated protein encoding single-stranded DNA (CRESS DNA) viruses are increasingly recognized worldwide in a variety of samples. Representative members include well-described veterinary pathogens with worldwide distribution, such as porcine circoviruses or beak and feather disease virus. In addition, numerous novel viruses belonging to the family Circoviridae with unverified pathogenic roles have been discovered in different human samples. Viruses of the family Genomoviridae have also been described as being highly abundant in different faecal and environmental samples, with case reports showing them to be suspected pathogens in human infections. In order to investigate the genetic diversity of these viruses in European bat populations, we tested guano samples from Georgia, Hungary, Romania, Serbia and Ukraine. This resulted in the detection of six novel members of the family Circoviridae and two novel members of the family Genomoviridae. Interestingly, a gemini-like virus, namely niminivirus, which was originally found in raw sewage samples in Nigeria, was also detected in our samples. We analyzed the nucleotide composition of members of the family Circoviridae to determine the possible host origins of these viruses. This study provides the first dataset on CRESS DNA viruses of European bats, and members of several novel viral species were discovered.
[Mh] Termos MeSH primário: Quirópteros/virologia
Circoviridae/genética
Infecções por Vírus de DNA/epidemiologia
Vírus de DNA/genética
DNA de Cadeia Simples/genética
DNA Viral/genética
Genoma Viral
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Circoviridae/classificação
Circoviridae/isolamento & purificação
Infecções por Vírus de DNA/transmissão
Infecções por Vírus de DNA/virologia
Vírus de DNA/classificação
Vírus de DNA/isolamento & purificação
Europa Oriental/epidemiologia
Fezes/virologia
Georgia/epidemiologia
Seres Humanos
Filogenia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Single-Stranded); 0 (DNA, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180220
[Lr] Data última revisão:
180220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171217
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3678-5


  2 / 1514 MEDLINE  
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[PMID]:29089143
[Au] Autor:Tang X; Liang Q; Liu L; Sheng X; Xing J; Zhan W
[Ad] Endereço:Laboratory of Pathology and Immunology of Aquatic Animals, KLM, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, No.1 Wenhai Road, Aoshanwei Town,
[Ti] Título:An optimized double-antibody sandwich ELISA for quantitative detection of WSSV in artificially infected crayfish.
[So] Source:J Virol Methods;251:133-138, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Developing a rapid, accurate and quantitative method for detecting white spot syndrome virus (WSSV) is extremely urgent and critical for reducing the risk of white spot disease outbreaks. In the present work, an optimized double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) was developed for quantitative detection of WSSV. The method employed rabbit polyclonal antibodies against WSSV as the capture antibody and previously produced anti-WSSV monoclonal antibodies as the detector antibody. A standard curve of the log concentration of WSSV versus OD value was established, which was linear in the concentration range of 120-7680ng/mL, and the linear regression equation was y=0.166x-0.151. Viral proteins in different tissues of crayfish (Procambarus clarkia) post artificial infection with WSSV were quantitatively measured using the DAS-ELISA. WSSV proliferated quickly within 60h post infection and gradually slowed down afterwards. According to the linear regression relationship, the viral proteins in hemolymph, gut and gonad were firstly able to be quantified at 24h post infection with the concentrations of 186, 158 and 128ng/mL, respectively. These three tissues also contained higher viral proteins than the gill, heart, hepatopancreas and muscle during the entire infection period. The viral protein concentration in gut reached the highest level of 6220ng/mL at 72h post infection. Real time quantitative PCR was also used to detect the dynamic change of viral copies in crayfish hemolymph post WSSV infection, with similar results for both assays. The developed DAS-ELISA could detect WSSV propagation from initial to moribund stage in infected crayfish and demonstrated potential application for diagnosis of WSSV.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Astacoidea/virologia
Infecções por Vírus de DNA/veterinária
Ensaio de Imunoadsorção Enzimática/métodos
Carga Viral/métodos
Vírus 1 da Síndrome da Mancha Branca/isolamento & purificação
[Mh] Termos MeSH secundário: Estruturas Animais/virologia
Animais
Infecções por Vírus de DNA/virologia
Coelhos
Fatores de Tempo
Vírus 1 da Síndrome da Mancha Branca/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171102
[St] Status:MEDLINE


  3 / 1514 MEDLINE  
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[PMID]:28962967
[Au] Autor:Gao F; Jiang JZ; Wang JY; Wei HY
[Ad] Endereço:Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; Shanghai Ocean University, Shanghai, 201306, China. Electronic address: tianchengyinuo@163.com.
[Ti] Título:Real-time isothermal detection of Abalone herpes-like virus and red-spotted grouper nervous necrosis virus using recombinase polymerase amplification.
[So] Source:J Virol Methods;251:92-98, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Abalone herpes-like virus (AbHV) and Red-spotted grouper nervous necrosis virus (RGNNV) are two serious viruses that infect animal populations in aquaculture. Both viruses cause diseases associated with high mortality rates, resulting in dramatic economic losses in the aquaculture industry. There are currently no effective treatments for either of these two viral diseases. Thus, early, rapid, and accurate diagnosis plays a fundamental role in disease prevention and control in aquaculture. Traditional methods of diagnosis, such as virus culture, enzyme-linked immunoassay, and polymerase chain reaction (PCR), are either time consuming or require sophisticated temperature control devices. In this study, one sets of specific primers and probes were designed for the real-time quantitative recombinase polymerase amplification (qRPA) detection of AbHV and RGNNV separately. The sensitivity and specificity of detection were evaluated by comparison with detection by conventional PCR and quantitative PCR. The optimal reaction temperature and time for virus detection is 37°C for 20min. The detection limit is 100 copies per reaction, making this approach faster and more sensitive than qPCR in this study. In a field application, the detection percentage of qRPA was higher than that of qPCR for both AbHV and NNV. Additionally, good correlation was found between qRPA and qPCR detection (R >0.8). The methods presented here can be used as alternatives to qPCR for quick and quantitative detection of pathogens infecting aquaculture species.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/veterinária
Herpesviridae/isolamento & purificação
Técnicas de Diagnóstico Molecular/métodos
Nodaviridae/isolamento & purificação
Técnicas de Amplificação de Ácido Nucleico/métodos
Infecções por Vírus de RNA/veterinária
[Mh] Termos MeSH secundário: Animais
Aquicultura
Infecções por Vírus de DNA/diagnóstico
Peixes
Gastrópodes
Herpesviridae/genética
Nodaviridae/genética
Infecções por Vírus de RNA/diagnóstico
Sensibilidade e Especificidade
Medicina Veterinária/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171001
[St] Status:MEDLINE


  4 / 1514 MEDLINE  
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[PMID]:28792686
[Au] Autor:Dixon SB; Lane A; O'Brien MM; Burns KC; Mangino JL; Breese EH; Absalon MJ; Perentesis JP; Phillips CL
[Ad] Endereço:Department of Pediatric Hematology and Oncology, St Jude Children's Research Hospital, Memphis, Tennessee.
[Ti] Título:Viral surveillance using PCR during treatment of AML and ALL.
[So] Source:Pediatr Blood Cancer;65(1), 2018 Jan.
[Is] ISSN:1545-5017
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: While viral surveillance of cytomegalovirus (CMV), Epstein-Barr virus (EBV), and adenovirus using PCR is routine in patients undergoing hematopoetic stem cell transplant and solid organ transplant, the utility in the nontransplant pediatric leukemia population is unknown. Our institution screens patients with acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) for viral DNAemia by PCR as part of clinical care. PROCEDURE: This retrospective chart review included patients treated for newly diagnosed or relapsed AML or ALL between April 2010 and September 2014. We retrieved data for viral PCR screening, detection and quantification, duration of positivity, and prophylaxis or treatment. RESULTS: One hundred eleven patients were included in analyses. Forty (36.0%) had at least one blood PCR positive for EBV, CMV, or adenovirus. Patients with ALL had significantly higher rates of persistent viral detection and treatment than those with AML (P < 0.02, P < 0.01, respectively). International patients had significantly higher rates of viral detection (P < 0.01), persistence (P < 0.01), any treatment (P < 0.03), and antiviral treatment (P < 0.01); 16.9% of patients who received intravenous immunoglobulin (IVIG) prophylactically had viral detection compared to 63% of patients who did not receive prophylactic IVIG (P = 0.0008). CONCLUSIONS: Patients with ALL were more susceptible than those with AML to viral reactivation that was persistent or resulted in treatment. Patients with relapsed ALL, refractory ALL, or infantile ALL are most likely to benefit from asymptomatic screening for CMV and adenovirus. International patients are at higher risk for reactivation and may merit screening. EBV reactivation was not significant and does not warrant screening.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/sangue
Vírus de DNA
DNA Viral/sangue
Leucemia Mieloide Aguda
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Criança
Pré-Escolar
Infecções por Vírus de DNA/prevenção & controle
Feminino
Seres Humanos
Imunoglobulinas Intravenosas/administração & dosagem
Lactente
Recém-Nascido
Leucemia Mieloide Aguda/sangue
Leucemia Mieloide Aguda/virologia
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Immunoglobulins, Intravenous)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171129
[Lr] Data última revisão:
171129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1002/pbc.26752


  5 / 1514 MEDLINE  
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[PMID]:28931029
[Au] Autor:Price SJ; Wadia A; Wright ON; Leung WTM; Cunningham AA; Lawson B
[Ad] Endereço:UCL Genetics Institute, Gower Street, London, United Kingdom.
[Ti] Título:Screening of a long-term sample set reveals two Ranavirus lineages in British herpetofauna.
[So] Source:PLoS One;12(9):e0184768, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Reports of severe disease outbreaks in amphibian communities in mainland Europe due to strains of the common midwife toad virus (CMTV)-like clade of Ranavirus are increasing and have created concern due to their considerable population impacts. In Great Britain, viruses in another clade of Ranavirus-frog virus 3 (FV3)-like-have caused marked declines of common frog (Rana temporaria) populations following likely recent virus introductions. The British public has been reporting mortality incidents to a citizen science project since 1992, with carcasses submitted for post-mortem examination, resulting in a long-term tissue archive spanning 25 years. We screened this archive for ranavirus (458 individuals from 228 incidents) using molecular methods and undertook preliminary genotyping of the ranaviruses detected. In total, ranavirus was detected in 90 individuals from 41 incidents focused in the north and south of England. The majority of detections involved common frogs (90%) but also another anuran, a caudate and a reptile. Most incidents were associated with FV3-like viruses but two, separated by 300 km and 16 years, involved CMTV-like viruses. These British CMTV-like viruses were more closely related to ranaviruses from mainland Europe than to each other and were estimated to have diverged at least 458 years ago. This evidence of a CMTV-like virus in Great Britain in 1995 represents the earliest confirmed case of a CMTV associated with amphibians and raises important questions about the history of ranavirus in Great Britain and the epidemiology of CMTV-like viruses. Despite biases present in the opportunistic sample used, this study also demonstrates the role of citizen science projects in generating resources for research and the value of maintaining long-term wildlife tissue archives.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/diagnóstico
Infecções por Vírus de DNA/veterinária
Ranavirus/genética
Ranavirus/isolamento & purificação
Répteis/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Vírus de DNA/epidemiologia
Infecções por Vírus de DNA/virologia
DNA Viral/análise
Filogenia
Ranavirus/classificação
Reino Unido/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170921
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184768


  6 / 1514 MEDLINE  
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[PMID]:28866775
[Au] Autor:Sobhy H
[Ad] Endereço:Department of Molecular Biology, Umeå University, 901 87, Umeå, Sweden. haithamsobhy@gmail.com.
[Ti] Título:A comparative review of viral entry and attachment during large and giant dsDNA virus infections.
[So] Source:Arch Virol;162(12):3567-3585, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Viruses enter host cells via several mechanisms, including endocytosis, macropinocytosis, and phagocytosis. They can also fuse at the plasma membrane and can spread within the host via cell-to-cell fusion or syncytia. The mechanism used by a given viral strain depends on its external topology and proteome and the type of cell being entered. This comparative review discusses the cellular attachment receptors and entry pathways of dsDNA viruses belonging to the families Adenoviridae, Baculoviridae, Herpesviridae and nucleocytoplasmic large DNA viruses (NCLDVs) belonging to the families Ascoviridae, Asfarviridae, Iridoviridae, Phycodnaviridae, and Poxviridae, and giant viruses belonging to the families Mimiviridae and Marseilleviridae as well as the proposed families Pandoraviridae and Pithoviridae. Although these viruses have several common features (e.g., topology, replication and protein sequence similarities) they utilize different entry pathways to infect wide-range of hosts, including humans, other mammals, invertebrates, fish, protozoa and algae. Similarities and differences between the entry methods used by these virus families are highlighted, with particular emphasis on viral topology and proteins that mediate viral attachment and entry. Cell types that are frequently used to study viral entry are also reviewed, along with other factors that affect virus-host cell interactions.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/virologia
Vírus de DNA/fisiologia
Ligação Viral
Internalização do Vírus
[Mh] Termos MeSH secundário: Animais
Seres Humanos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170904
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3497-8


  7 / 1514 MEDLINE  
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[PMID]:28850602
[Au] Autor:Milrot E; Shimoni E; Dadosh T; Rechav K; Unger T; Van Etten JL; Minsky A
[Ad] Endereço:Department of Structural Biology, The Weizmann Institute of Science, Rehovot, Israel.
[Ti] Título:Structural studies demonstrating a bacteriophage-like replication cycle of the eukaryote-infecting Paramecium bursaria chlorella virus-1.
[So] Source:PLoS Pathog;13(8):e1006562, 2017 Aug.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A fundamental stage in viral infection is the internalization of viral genomes in host cells. Although extensively studied, the mechanisms and factors responsible for the genome internalization process remain poorly understood. Here we report our observations, derived from diverse imaging methods on genome internalization of the large dsDNA Paramecium bursaria chlorella virus-1 (PBCV-1). Our studies reveal that early infection stages of this eukaryotic-infecting virus occurs by a bacteriophage-like pathway, whereby PBCV-1 generates a hole in the host cell wall and ejects its dsDNA genome in a linear, base-pair-by-base-pair process, through a membrane tunnel generated by the fusion of the virus internal membrane with the host membrane. Furthermore, our results imply that PBCV-1 DNA condensation that occurs shortly after infection probably plays a role in genome internalization, as hypothesized for the infection of some bacteriophages. The subsequent perforation of the host photosynthetic membranes presumably enables trafficking of viral genomes towards host nuclei. Previous studies established that at late infection stages PBCV-1 generates cytoplasmic organelles, termed viral factories, where viral assembly takes place, a feature characteristic of many large dsDNA viruses that infect eukaryotic organisms. PBCV-1 thus appears to combine a bacteriophage-like mechanism during early infection stages with a eukaryotic-like infection pathway in its late replication cycle.
[Mh] Termos MeSH primário: Chlorella/virologia
Infecções por Vírus de DNA
Interações Hospedeiro-Parasita/fisiologia
Phycodnaviridae/fisiologia
Montagem de Vírus/fisiologia
[Mh] Termos MeSH secundário: DNA Viral/fisiologia
Imunofluorescência
Imagem Tridimensional
Microscopia Eletrônica de Transmissão
Phycodnaviridae/ultraestrutura
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006562


  8 / 1514 MEDLINE  
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[PMID]:28818331
[Au] Autor:Hick PM; Subramaniam K; Thompson PM; Waltzek TB; Becker JA; Whittington RJ
[Ad] Endereço:OIE Reference Laboratory for Epizootic Haematopoietic Necrosis Virus and Ranavirus Infection of Amphibians, Sydney School of Veterinary Science and School of Life and Environmental Sciences, The University Sydney, Werombi Road, Camden 2570, NSW, Australia. Electronic address: paul.hick@sydney.edu.au
[Ti] Título:Molecular epidemiology of Epizootic haematopoietic necrosis virus (EHNV).
[So] Source:Virology;511:320-329, 2017 Nov.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Low genetic diversity of Epizootic haematopoietic necrosis virus (EHNV) was determined for the complete genome of 16 isolates spanning the natural range of hosts, geography and time since the first outbreaks of disease. Genomes ranged from 125,591-127,487 nucleotides with 97.47% pairwise identity and 106-109 genes. All isolates shared 101 core genes with 121 potential genes predicted within the pan-genome of this collection. There was high conservation within 90,181 nucleotides of the core genes with isolates separated by average genetic distance of 3.43 × 10 substitutions per site. Evolutionary analysis of the core genome strongly supported historical epidemiological evidence of iatrogenic spread of EHNV to naïve hosts and establishment of endemic status in discrete ecological niches. There was no evidence of structural genome reorganization, however, the complement of non-core genes and variation in repeat elements enabled fine scale molecular epidemiological investigation of this unpredictable pathogen of fish.
[Mh] Termos MeSH primário: Surtos de Doenças
Doenças dos Peixes/epidemiologia
Doenças dos Peixes/virologia
Variação Genética
Epidemiologia Molecular
Ranavirus/classificação
Ranavirus/genética
[Mh] Termos MeSH secundário: Animais
Infecções por Vírus de DNA/veterinária
Infecções por Vírus de DNA/virologia
Doenças Endêmicas
Peixes
Genes Virais
Genoma Viral
Doença Iatrogênica/epidemiologia
Doença Iatrogênica/veterinária
Ranavirus/isolamento & purificação
Análise de Sequência de DNA
Homologia de Sequência
Sintenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170819
[St] Status:MEDLINE


  9 / 1514 MEDLINE  
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[PMID]:28803676
[Au] Autor:Claytor SC; Subramaniam K; Landrau-Giovannetti N; Chinchar VG; Gray MJ; Miller DL; Mavian C; Salemi M; Wisely S; Waltzek TB
[Ad] Endereço:Department of Wildlife Ecology and Conservation, University of Florida, Gainesville, FL, USA.
[Ti] Título:Ranavirus phylogenomics: Signatures of recombination and inversions among bullfrog ranaculture isolates.
[So] Source:Virology;511:330-343, 2017 Nov.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ranaviruses are emerging pathogens of fish, amphibians, and reptiles that threaten aquatic animal industries and wildlife worldwide. Our objective was to genetically characterize ranaviruses isolated during separate bullfrog Lithobates catesbeianus die-offs that occurred eight years apart on the same North American farm. The earlier outbreak was due to a highly pathogenic strain of common midwife toad virus (CMTV) previously known only from Europe and China. The later outbreak was due to a chimeric ranavirus that displayed a novel genome arrangement and a DNA backbone typical for Frog virus 3 (FV3) strains except for interspersed fragments acquired through recombination with the CMTV isolated earlier. Both bullfrog ranaviruses are more pathogenic than wild-type FV3 suggesting recombination may have resulted in the increased pathogenicity observed in the ranavirus isolated in the later outbreak. Our study underscores the role international trade in farmed bullfrogs may have played in the global dissemination of highly pathogenic ranaviruses.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/veterinária
Variação Genética
Ranavirus/classificação
Ranavirus/genética
Recombinação Genética
Inversão de Sequência
[Mh] Termos MeSH secundário: Animais
Infecções por Vírus de DNA/virologia
DNA Viral/química
DNA Viral/genética
América do Norte
Rana catesbeiana/virologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE


  10 / 1514 MEDLINE  
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[PMID]:28783452
[Au] Autor:Tzannou I; Papadopoulou A; Naik S; Leung K; Martinez CA; Ramos CA; Carrum G; Sasa G; Lulla P; Watanabe A; Kuvalekar M; Gee AP; Wu MF; Liu H; Grilley BJ; Krance RA; Gottschalk S; Brenner MK; Rooney CM; Heslop HE; Leen AM; Omer B
[Ad] Endereço:Ifigeneia Tzannou, Anastasia Papadopoulou, Swati Naik, Kathryn Leung, Caridad A. Martinez, Carlos A. Ramos, George Carrum, Ghadir Sasa, Premal Lulla, Ayumi Watanabe, Manik Kuvalekar, Adrian P. Gee, Bambi J. Grilley, Robert A. Krance, Stephen Gottschalk, Malcolm K. Brenner, Cliona M. Rooney, Helen E.
[Ti] Título:Off-the-Shelf Virus-Specific T Cells to Treat BK Virus, Human Herpesvirus 6, Cytomegalovirus, Epstein-Barr Virus, and Adenovirus Infections After Allogeneic Hematopoietic Stem-Cell Transplantation.
[So] Source:J Clin Oncol;35(31):3547-3557, 2017 Nov 01.
[Is] ISSN:1527-7755
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose Improvement of cure rates for patients treated with allogeneic hematopoietic stem-cell transplantation (HSCT) will require efforts to decrease treatment-related mortality from severe viral infections. Adoptively transferred virus-specific T cells (VSTs) generated from eligible, third-party donors could provide broad antiviral protection to recipients of HSCT as an immediately available off-the-shelf product. Patient and Methods We generated a bank of VSTs that recognized five common viral pathogens: Epstein-Barr virus (EBV), adenovirus (AdV), cytomegalovirus (CMV), BK virus (BKV), and human herpesvirus 6 (HHV-6). The VSTs were administered to 38 patients with 45 infections in a phase II clinical trial. Results A single infusion produced a cumulative complete or partial response rate of 92% (95% CI, 78.1% to 98.3%) overall and the following rates by virus: 100% for BKV (n = 16), 94% for CMV (n = 17), 71% for AdV (n = 7), 100% for EBV (n = 2), and 67% for HHV-6 (n = 3). Clinical benefit was achieved in 31 patients treated for one infection and in seven patients treated for multiple coincident infections. Thirteen of 14 patients treated for BKV-associated hemorrhagic cystitis experienced complete resolution of gross hematuria by week 6. Infusions were safe, and only two occurrences of de novo graft-versus host disease (grade 1) were observed. VST tracking by epitope profiling revealed persistence of functional VSTs of third-party origin for up to 12 weeks. Conclusion The use of banked VSTs is a feasible, safe, and effective approach to treat severe and drug-refractory infections after HSCT, including infections from two viruses (BKV and HHV-6) that had never been targeted previously with an off-the-shelf product. Furthermore, the multispecificity of the VSTs ensures extensive antiviral coverage, which facilitates the treatment of patients with multiple infections.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/terapia
Vírus de DNA/imunologia
Transplante de Células-Tronco Hematopoéticas/efeitos adversos
Transplante de Células-Tronco Hematopoéticas/métodos
Imunoterapia Adotiva/métodos
Linfócitos T/imunologia
Linfócitos T/transplante
[Mh] Termos MeSH secundário: Adenovírus Humanos/imunologia
Adulto
Vírus BK/imunologia
Infecções por Vírus de DNA/etiologia
Infecções por Vírus de DNA/virologia
Feminino
Herpesvirus Humano 4/imunologia
Herpesvirus Humano 6/imunologia
Seres Humanos
Masculino
Transplante Homólogo
Resultado do Tratamento
[Pt] Tipo de publicação:CLINICAL TRIAL, PHASE II; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171117
[Lr] Data última revisão:
171117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE
[do] DOI:10.1200/JCO.2017.73.0655



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