Base de dados : MEDLINE
Pesquisa : C02.256.430 [Categoria DeCS]
Referências encontradas : 224 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 23 ir para página                         

  1 / 224 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28152393
[Au] Autor:Liu Y; Wang J; Wang L; Wang B; Yang S; Wang Q; Luo J; Feng X; Yang X; Lu Y; Roggendorf M; Lu M; Yang D; Liu J
[Ad] Endereço:Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
[Ti] Título:Molecular cloning, characterization and expression analysis of Tim-3 and Galectin-9 in the woodchuck model.
[So] Source:Mol Immunol;83:127-136, 2017 Mar.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In recent years, a critical role for T cell immunoglobulin mucin domain 3 (Tim-3) and its ligand Galectin-9 (Gal-9) has emerged in infectious disease, autoimmunity and cancer. Manipulating this immune checkpoint may have immunotherapeutic potential and could represent an alternative approach for improving immune responses to viral infections and cancer. The woodchuck (Marmot monax) infected by woodchuck hepatitis virus (WHV) represents an informative animal model to study HBV infection and HCC. In the current study, the cDNA sequences of woodchuck Tim-3 and Gal-9 were cloned, sequenced and characterized. The extracellular domain of Tim-3 cDNA sequence consisted of 576bp coding sequence (CDS) that encoded 192 amino acids. The 1076bp full-length Gal-9 cDNA sequence consisted of 1059bp coding sequence (CDS) that encoded 352 amino acids with a molecular weight of 39.7kDa. The phylogenetic tree analysis revealed that the woodchuck Tim-3 and Gal-9 had the closest genetic relationship with Ictidomys tridecemlineatus. The result of quantification PCR analysis showed that ubiquitous expression of Gal-9 but not Tim-3 in different tissues of naive woodchucks. Elevated liver Gal-9 expression was observed in woodchucks with chronic WHV infection. Moreover, a polyclonal antibody against the extracellular domain of woodchuck Tim-3 were generated and identified by flow cytometry. Our results serve as a foundation for further insight into the role of Tim-3/Galectin-9 signaling pathway in viral hepatitis and HCC in the woodchuck model.
[Mh] Termos MeSH primário: Galectinas/genética
Receptor Celular 2 do Vírus da Hepatite A/genética
Marmota/genética
Marmota/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Clonagem Molecular
DNA Complementar/análise
DNA Complementar/genética
Galectinas/biossíntese
Infecções por Hepadnaviridae/genética
Infecções por Hepadnaviridae/imunologia
Receptor Celular 2 do Vírus da Hepatite A/biossíntese
Vírus da Hepatite B da Marmota
Hepatite Viral Animal/genética
Hepatite Viral Animal/imunologia
Filogenia
Reação em Cadeia da Polimerase
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Galectins); 0 (Hepatitis A Virus Cellular Receptor 2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE


  2 / 224 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27501758
[Au] Autor:Shen Z; Liu Y; Luo M; Wang W; Liu J; Liu W; Pan S; Xie Y
[Ad] Endereço:Key Laboratory of Medical Molecular Virology (Ministry of Health and Ministry of Education), Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200031, China.
[Ti] Título:Nuclear factor Y regulates ancient budgerigar hepadnavirus core promoter activity.
[So] Source:Biochem Biophys Res Commun;478(2):825-30, 2016 09 16.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Endogenous viral elements (EVE) in animal genomes are the fossil records of ancient viruses and provide invaluable information on the origin and evolution of extant viruses. Extant hepadnaviruses include avihepadnaviruses of birds and orthohepadnaviruses of mammals. The core promoter (Cp) of hepadnaviruses is vital for viral gene expression and replication. We previously identified in the budgerigar genome two EVEs that contain the full-length genome of an ancient budgerigar hepadnavirus (eBHBV1 and eBHBV2). Here, we found eBHBV1 Cp and eBHBV2 Cp were active in several human and chicken cell lines. A region from nt -85 to -11 in eBHBV1 Cp was critical for the promoter activity. Bioinformatic analysis revealed a putative binding site of nuclear factor Y (NF-Y), a ubiquitous transcription factor, at nt -64 to -50 in eBHBV1 Cp. The NF-Y core binding site (ATTGG, nt -58 to -54) was essential for eBHBV1 Cp activity. The same results were obtained with eBHBV2 Cp and duck hepatitis B virus Cp. The subunit A of NF-Y (NF-YA) was recruited via the NF-Y core binding site to eBHBV1 Cp and upregulated the promoter activity. Finally, the NF-Y core binding site is conserved in the Cps of all the extant avihepadnaviruses but not of orthohepadnaviruses. Interestingly, a putative and functionally important NF-Y core binding site is located at nt -21 to -17 in the Cp of human hepatitis B virus. In conclusion, our findings have pinpointed an evolutionary conserved and functionally critical NF-Y binding element in the Cps of avihepadnaviruses.
[Mh] Termos MeSH primário: Fator de Ligação a CCAAT/genética
Elementos de DNA Transponíveis
DNA Viral/genética
Genoma
Hepadnaviridae/genética
Hepatócitos/metabolismo
[Mh] Termos MeSH secundário: Animais
Sítios de Ligação
Evolução Biológica
Doenças das Aves/virologia
Fator de Ligação a CCAAT/química
Fator de Ligação a CCAAT/metabolismo
Linhagem Celular
Linhagem Celular Tumoral
Embrião de Galinha
Galinhas
Sequência Conservada
DNA Viral/metabolismo
Extinção Biológica
Fibroblastos/metabolismo
Fibroblastos/virologia
Fósseis
Células HEK293
Hepadnaviridae/classificação
Hepadnaviridae/metabolismo
Infecções por Hepadnaviridae/veterinária
Infecções por Hepadnaviridae/virologia
Vírus da Hepatite B/genética
Vírus da Hepatite B/metabolismo
Hepatócitos/virologia
Interações Hospedeiro-Patógeno
Seres Humanos
Melopsittacus
Filogenia
Regiões Promotoras Genéticas
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CCAAT-Binding Factor); 0 (DNA Transposable Elements); 0 (DNA, Viral); 0 (nuclear factor Y)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171127
[Lr] Data última revisão:
171127
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160810
[St] Status:MEDLINE


  3 / 224 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26900848
[Au] Autor:Yan L; Qu S; Liu G; Liu L; Yu Y; Ding G; Zhao Y; Li Y; Xie Y; Zhang J; Qu D
[Ad] Endereço:Key Laboratory of Medical Molecular Virology, Ministry of Education and Health, School of Basic Medical Sciences, Fudan University, Shanghai, China.
[Ti] Título:Comparative Transcriptomic Analysis of Primary Duck Hepatocytes Provides Insight into Differential Susceptibility to DHBV Infection.
[So] Source:PLoS One;11(2):e0149702, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Primary duck hepatocytes (PDH) displays differential susceptibility to duck hepatitis B virus when maintained in the media supplemented with fetal bovine serum or dimethyl sulfoxide (DMSO) which has been widely used for the maintenance of hepatocytes, and prolonging susceptibility to hepadnavirus. However the mechanism underlying maintenance of susceptibility to hepadnavirus by DMSO treatment remains unclear. In this study, a global transcriptome analysis of PDHs under different culture conditions was conducted for investigating the effects of DMSO on maintenance of susceptibility of PDH to DHBV in vitro. The 384 differential expressed genes (DEGs) were identified by comparisons between each library pair (PDHs cultured with or without DMSO or fresh isolated PDH). We analyzed canonical pathways in which the DEGs were enriched in Hepatic Fibrosis / Hepatic Stellate Cell Activation, Bile Acid Biosynthesis and Tight Junction signaling. After re-annotation against human genome data, the 384 DEGs were pooled together with proteins belonging to hepatitis B pathway to construct a protein-protein interaction network. The combination of decreased expression of liver-specific genes (CYP3A4, CYP1E1, CFI, RELN and GSTA1 et al) with increased expression of hepatocyte-dedifferentiation-associated genes (PLA2G4A and PLCG1) suggested that in vitro culture conditions results in the fading of hepatocyte phenotype in PDHs. The expression of seven DEGs associated with tight junction formation (JAM3, PPP2R2B, PRKAR1B, PPP2R2C, MAGI2, ACTA2 and ACTG2) was up-regulated after short-term culture in vitro, which was attenuated in the presence of DMSO. Those results could shed light on DHBV infection associated molecular events affected by DMSO.
[Mh] Termos MeSH primário: Patos/virologia
Perfilação da Expressão Gênica/métodos
Infecções por Hepadnaviridae/genética
Infecções por Hepadnaviridae/veterinária
Vírus da Hepatite B do Pato/fisiologia
Hepatite Viral Animal/genética
Hepatócitos/virologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Análise por Conglomerados
DNA Viral/metabolismo
Suscetibilidade a Doenças
Patos/genética
Regulação da Expressão Gênica
Infecções por Hepadnaviridae/virologia
Hepatócitos/metabolismo
Análise de Componente Principal
Mapeamento de Interação de Proteínas
Reação em Cadeia da Polimerase em Tempo Real
Reprodutibilidade dos Testes
Análise de Sequência de DNA
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160223
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0149702


  4 / 224 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26509653
[Au] Autor:Fu J; Guo D; Gao D; Huang W; Li Z; Jia B
[Ad] Endereço:Key Laboratory of Infectious and Parasitic Diseases in Chongqing, Department of Infectious Diseases, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
[Ti] Título:Clinical analysis of patients suffering from chronic hepatitis B superinfected with other hepadnaviruses.
[So] Source:J Med Virol;88(6):1003-9, 2016 Jun.
[Is] ISSN:1096-9071
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To compare the clinical manifestations, laboratory examinations, and prognoses of patients with chronic hepatitis B (CHB) who were superinfected with hepatitis A virus (HAV), hepatitis C virus (HCV), hepatitis D virus (HDV), or hepatitis E virus (HEV). Two hundred and eleven patients with confirmed CHB in our hospital, a tertiary teaching hospital in China, between 2005 and 2014 were analyzed retrospectively. Among 211 patients with CHB, 35 were superinfected with HAV, 31 were superinfected with HCV, 22 were superinfected with HDV, and 53 were superinfected with HEV. We analyzed and compared the clinical features of the five groups. The tested biochemical indices and markers of liver function included serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBil), prothrombin activity (PTA), serum albumin (Alb), and the serum levels of HBV DNA. The peak values of ALT, AST, and TBil were significantly higher in all of the superinfected groups. Lower peak Alb concentration and PTA were also observed in the superinfected patients, with the exception of patients in the CHB + HAV group. The CHB + HCV, and CHB + HEV groups had higher death rates than the CHB monoinfected group, and the difference was statistically significant. Further analysis of the liver failure groups showed that the level of HBV DNA was not correlated with prognosis. The comparison of clinical outcomes revealed that CHB patients superinfected with HCV, HDV, and HEV compared with CHB monoinfection had statistically greater incidences of exacerbation of the condition and poor prognosis, whereas the patients superinfected with HAV generally had better outcomes.
[Mh] Termos MeSH primário: Infecções por Hepadnaviridae/fisiopatologia
Hepatite B Crônica/fisiopatologia
Falência Hepática/virologia
Superinfecção/fisiopatologia
[Mh] Termos MeSH secundário: Adulto
Alanina Transaminase/sangue
Aspartato Aminotransferases/sangue
China/epidemiologia
DNA Viral/sangue
Feminino
Hepacivirus/fisiologia
Infecções por Hepadnaviridae/mortalidade
Infecções por Hepadnaviridae/virologia
Antígenos E da Hepatite B/sangue
Vírus da Hepatite B/genética
Hepatite B Crônica/diagnóstico
Hepatite B Crônica/epidemiologia
Hepatite B Crônica/virologia
Hepatite C Crônica/epidemiologia
Hepatite C Crônica/fisiopatologia
Hepatite C Crônica/virologia
Hepatite E/epidemiologia
Hepatite E/fisiopatologia
Hepatite E/virologia
Vírus da Hepatite E/fisiologia
Seres Humanos
Testes de Função Hepática
Masculino
Meia-Idade
Prognóstico
Estudos Retrospectivos
Superinfecção/diagnóstico
Superinfecção/epidemiologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Hepatitis B e Antigens); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151029
[St] Status:MEDLINE
[do] DOI:10.1002/jmv.24417


  5 / 224 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25833941
[Au] Autor:Mason WS
[Ad] Endereço:Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111.
[Ti] Título:Animal models and the molecular biology of hepadnavirus infection.
[So] Source:Cold Spring Harb Perspect Med;5(4), 2015 Apr 01.
[Is] ISSN:2157-1422
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Australian antigen, the envelope protein of hepatitis B virus (HBV), was discovered in 1967 as a prevalent serum antigen in hepatitis B patients. Early electron microscopy (EM) studies showed that this antigen was present in 22-nm particles in patient sera, which were believed to be incomplete virus. Complete virus, much less abundant than the 22-nm particles, was finally visualized in 1970. HBV was soon found to infect chimpanzees, gorillas, orangutans, gibbon apes, and, more recently, tree shrews (Tupaia belangeri) and cynomolgus macaques (Macaca fascicularis). This restricted host range placed limits on the kinds of studies that might be performed to better understand the biology and molecular biology of HBV and to develop antiviral therapies to treat chronic infections. About 10 years after the discovery of HBV, this problem was bypassed with the discovery of viruses related to HBV in woodchucks, ground squirrels, and ducks. Although unlikely animal models, their use revealed the key steps in hepadnavirus replication and in the host response to infection, including the fact that the viral nuclear episome is the ultimate target for immune clearance of transient infections and antiviral therapy of chronic infections. Studies with these and other animal models have also suggested interesting clues into the link between chronic HBV infection and hepatocellular carcinoma.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Infecções por Hepadnaviridae/virologia
Hepadnaviridae/genética
[Mh] Termos MeSH secundário: Animais
DNA Viral/biossíntese
Genoma Viral
Hepadnaviridae/classificação
Hepadnaviridae/fisiologia
Vírus da Hepatite B do Pato
Hepatite B Crônica/virologia
Seres Humanos
Neoplasias Hepáticas/virologia
Orthohepadnavirus
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150403
[St] Status:MEDLINE


  6 / 224 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25449362
[Au] Autor:Liu Q; Huang J; Jia R; Wang M; Zhu D; Chen S; Liu M; Yin Z; Wang Y; Cheng A
[Ad] Endereço:Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Avian Disease Research Center, Sichuan Agricultural University, 46 Xinkang Road, Ya'an, Sichuan 625014, PR China.
[Ti] Título:The pregenome/C RNA of duck hepatitis B virus is not used for translation of core protein during the early phase of infection in vitro.
[So] Source:Virus Res;196:13-9, 2015 Jan 22.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Over the course of duck hepatitis B virus (DHBV) replication, one type of RNA (pregenome/C RNA, 3.5 kb) that corresponds to the whole genome of DHBV is generated from the transcription of viral cccDNA. Previous work has proposed three functions for the pregenome/C RNA: it can serve as the pregenome and be packaged into the core protein during the process of replication, and it encodes the mRNA for both the capsid protein and the viral polymerase. However, little is known about the timing of these functions during the different stages of viral infection. In this study, a reverse transcription quantitative real-time PCR assay was developed to analyze the dynamic transcription process of the pregenome/C RNA. The dynamic expression of the core protein was investigated using an indirect immunofluorescence assay (IFA) and by western blot analysis. The generation of pregenome/C RNA began at 12 h post infection and peaked at 20 h post infection; however, the core protein was not detectable until 24h post infection. These results demonstrate that the core protein appeared approximately 12h later than the pregenome/C RNA. These results suggest that the DHBV pregenome/C RNA is not used for the translation of the viral core protein during the early stages of infection.
[Mh] Termos MeSH primário: Vírus da Hepatite B do Pato/genética
Biossíntese de Proteínas
RNA Viral/genética
Proteínas do Core Viral/genética
[Mh] Termos MeSH secundário: Animais
Patos
Expressão Gênica
Genoma Viral
Infecções por Hepadnaviridae
Vírus da Hepatite B do Pato/metabolismo
Hepatite Viral Animal
RNA Viral/metabolismo
Fatores de Tempo
Transcrição Genética
Proteínas do Core Viral/metabolismo
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral); 0 (Viral Core Proteins)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150113
[Lr] Data última revisão:
150113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


  7 / 224 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25446633
[Au] Autor:Wei PH; Wu SZ; Mu XM; Xu B; Su QJ; Wei JL; Yang Y; Qin B; Xie ZC
[Ad] Endereço:Department of Epidemiology, Public Health School, Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
[Ti] Título:Effect of alcohol extract of Acanthus ilicifolius L. on anti-duck hepatitis B virus and protection of liver.
[So] Source:J Ethnopharmacol;160:1-5, 2015 Feb 03.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Acanthus ilicifolius L. is an important medicinal mangrove plant. It is popularly used for its anti-inflammatory, antioxidant activity and hepatoprotective effects. The present study was conducted to evaluate the effect of treatment with alcohol extract of Acanthus ilicifolius L. on duck hepatitis B. MATERIALS AND METHODS: One-day-old Guangxi shelducks injected intraperitoneally with strong positive duck hepatitis B virus (DHBV) serum were used to establish a duck hepatitis B animal model in the study. The ducks were respectively administered in different groups with low-, middle- and high-dose alcohol extracts of Acanthus ilicifolius L., the positive control drug acyclovir (ACV) and double-distilled water. The levels of serum DHBV DNA were detected by fluorescence quantitative PCR (FQ-PCR). Duck hepatitis B surface antigen (DHBsAg) and duck hepatitis B e antigen (DHBeAg) OD values in the serum were measured by ELISA. The activity of Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) in the serum was measured, and the livers were taken for histopathological examination. RESULTS: The levels of serum DHBV DNA and the values of DHBsAg and DHBeAg OD were not significant in any of the dose extract groups. However, the ALT activity was obviously lower in the middle- and high-dose extract groups. It was also found that a high dose of alcohol extract could reduce the activity of AST significantly and significantly improve hepatic pathological effects. CONCLUSIONS: High-dose alcohol extract of Acanthus ilicifolius L. has an obvious protective effect on the liver function and liver tissue. However, the present study finds that Acanthus ilicifolius L. cannot inhibit the replication of duck hepatitis B virus.
[Mh] Termos MeSH primário: Acanthaceae/química
Medicamentos de Ervas Chinesas/farmacologia
Medicamentos de Ervas Chinesas/uso terapêutico
Infecções por Hepadnaviridae/tratamento farmacológico
Vírus da Hepatite B do Pato/efeitos dos fármacos
Hepatite Viral Animal/tratamento farmacológico
Fígado/efeitos dos fármacos
Fitoterapia
[Mh] Termos MeSH secundário: Aciclovir/farmacologia
Aciclovir/uso terapêutico
Alanina Transaminase/sangue
Animais
Animais Recém-Nascidos
Antivirais/farmacologia
Antivirais/uso terapêutico
Aspartato Aminotransferases/sangue
DNA Viral/sangue
Relação Dose-Resposta a Droga
Medicamentos de Ervas Chinesas/química
Patos
Infecções por Hepadnaviridae/patologia
Antígenos de Superfície da Hepatite B/sangue
Antígenos E da Hepatite B/sangue
Hepatite Viral Animal/patologia
Fígado/patologia
Carga Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (DNA, Viral); 0 (Drugs, Chinese Herbal); 0 (Hepatitis B Surface Antigens); 0 (Hepatitis B e Antigens); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase); X4HES1O11F (Acyclovir)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:141230
[Lr] Data última revisão:
141230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


  8 / 224 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25193071
[Au] Autor:He B; Zhang F; Xia L; Hu T; Chen G; Qiu W; Fan Q; Feng Y; Guo H; Tu C
[Ad] Endereço:Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, 666 Liuying West Road, Jingyue Economic Development Zone, Changchun, 130122, People's Republic of China.
[Ti] Título:Identification of a novel Orthohepadnavirus in pomona roundleaf bats in China.
[So] Source:Arch Virol;160(1):335-7, 2015 Jan.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bats in Myanmar, Gabon, and Panama have been found to harbor diverse hepadnaviruses. Here, we report a novel hepadnavirus in 4 of 20 pomona roundleaf bats from Yunnan province, China. This virus contains 3,278 nucleotides (nt) in the full circularized genome, with four predicted open frames (ORFs) reading in the same direction. Full genomic sequence comparisons and evolutionary analysis indicate that this virus is a member of a new species within the genus Orthohepadnavirus.
[Mh] Termos MeSH primário: Quirópteros
Infecções por Hepadnaviridae/veterinária
Hepatite Viral Animal/virologia
Orthohepadnavirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Evolução Biológica
China/epidemiologia
Variação Genética
Infecções por Hepadnaviridae/epidemiologia
Infecções por Hepadnaviridae/virologia
Hepatite Viral Animal/epidemiologia
Orthohepadnavirus/genética
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1503
[Cu] Atualização por classe:150106
[Lr] Data última revisão:
150106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140907
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-014-2222-0


  9 / 224 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25457362
[Au] Autor:Bidin M; Tisljar M; Bidin Z; Lojkic I; Majnaric D
[Ad] Endereço:Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia. Electronic address: mbidin@vef.hr.
[Ti] Título:Genetic characterization of hepadnaviruses associated with histopathological changes in the liver of duck and goose embryos.
[So] Source:Vet Microbiol;174(3-4):302-308, 2014 Dec 05.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Avian hepadnaviruses are etiological agents of hepatitis B, that has been identified primarily in ducks, and more recently in various avian species. In this paper, 16 hepadnaviruses were detected by polymerase chain reaction (PCR) in the field samples from dead embryos of commercially reared domestic duck and goose. Based on the molecular analysis of the S-protein gene sequences and phylogenetic Neighbor-joining tree, identified viruses were clustered in the same genetic group, indicating no host-related diversity. Both duck and goose-origin hepadnaviruses were grouped within the cluster consisting of "Western-country" and "Chinese" duck hepatitis B (DHBV) isolates, showing more evolutionary distances with other known avian hepadnaviruses. Histopathologically, the lesions observed in the liver tissue from hepadnavirus positive duck and goose embryos varied from low to mild degree of perivascular mononuclear cells and mixed cell infiltrations, followed by mild vacuolar changes. Small focal necrotic changes in the liver parenchyma, and bile ductular proliferation were also found in examined liver samples. Generally, the microscopic findings resemble those described in experimentally infected ducks, while this was the first description of hepadnavirus associated lesions in domestic goose. Although hepadnaviruses are considered to have a very narrow host range, this study showed that domestic ducks and geese are susceptible to infection with genetically almost identical hepadnaviruses, that were likely to produce similar microscopic changes in the liver of both duck and goose embryos. The impact of naturally occurred hepadnavirus infection and possible synergistic interactions with other infectious or non-infectious agents on embryo viability needs further investigation.
[Mh] Termos MeSH primário: Patos/virologia
Gansos/virologia
Infecções por Hepadnaviridae/veterinária
Hepadnaviridae/genética
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Patos/embriologia
Gansos/embriologia
Hepadnaviridae/isolamento & purificação
Infecções por Hepadnaviridae/patologia
Infecções por Hepadnaviridae/virologia
Fígado/embriologia
Fígado/patologia
Fígado/virologia
Dados de Sequência Molecular
Filogenia
Doenças das Aves Domésticas/patologia
Análise de Sequência de DNA/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141203
[St] Status:MEDLINE


  10 / 224 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
[PMID]:25409748
[Au] Autor:Yang Q; Zhao X; Yu W; He W; Fang X; Zang L; Wan N; Wang Q; Zheng L; Chang J
[Ad] Endereço:Academy of Medical and Pharmaceutical Sciences, Zhengzhou University, China.
[Ti] Título:Anti-hepatitis B virus activity of α-DDB-FNCG, a novel nucleoside-biphenyldicarboxylate compound in vitro and in vivo.
[So] Source:J Pharmacol Sci;126(3):208-15, 2014.
[Is] ISSN:1347-8648
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:A novel codrug, α-DDB-FNCG, was synthesized through coupling of α-biphenyl dimethyl dicarboxylate (α-DDB) and the nucleoside analogue FNCG, via an ester bond. The anti-HBV activity and hepatoprotective effects of this compound were investigated both in vitro and in vivo. In HBV-transfected HepG2.2.15 cell line, the secretion of HBsAg and HBeAg as well as the levels of extracellular and intracellular viral DNA were determined by ELISA and real-time fluorescent quantitative Polymerase Chain Reaction (FQ-PCR), respectively. In DHBV-infected ducks, the viral DNA levels in serum and liver were determined by FQ-PCR. In addition, the levels of alanine transaminase (ALT) and aspartate aminotransferase (AST) in both serum and liver were also examined. The improvement of ducks' livers was evaluated by histopathological analysis. It has been demonstrated that α-DDB-FNCG could suppress the levels of HBV antigens and viral DNA in a time- and dose-dependent manner in the HepG2.2.15 cell line. Furthermore, this codrug could also significantly inhibit the viral DNA replication and reduce the ALT and AST levels in both serum and liver of DHBV-infected ducks, with improved hepatocellular architecture in drug-treated ducks. In short, these results suggest that α-DDB-FNCG could be a promising candidate for further development of new anti-HBV agents with hepatoprotective effects.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Benzodioxóis/farmacologia
Citidina/análogos & derivados
Infecções por Hepadnaviridae/tratamento farmacológico
Vírus da Hepatite B do Pato/efeitos dos fármacos
Vírus da Hepatite B/efeitos dos fármacos
Hepatite Viral Animal/tratamento farmacológico
Fígado/efeitos dos fármacos
[Mh] Termos MeSH secundário: Alanina Transaminase/sangue
Animais
Aspartato Aminotransferases/sangue
Citidina/farmacologia
DNA Viral/metabolismo
Relação Dose-Resposta a Droga
Patos
Feminino
Células Hep G2
Infecções por Hepadnaviridae/sangue
Infecções por Hepadnaviridae/patologia
Infecções por Hepadnaviridae/virologia
Antígenos de Superfície da Hepatite B/metabolismo
Vírus da Hepatite B do Pato/genética
Antígenos E da Hepatite B/metabolismo
Vírus da Hepatite B/genética
Vírus da Hepatite B/imunologia
Hepatite Viral Animal/sangue
Hepatite Viral Animal/patologia
Hepatite Viral Animal/virologia
Seres Humanos
Fígado/enzimologia
Fígado/patologia
Fígado/virologia
Masculino
Fatores de Tempo
Transfecção
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (4'-azido-2'-deoxy-2-fluoro-3'-(4,4'-dimethoxy-2'-ethoxycarbonyl-5,6,5',6'-bis(methylenedioxy)-1,1'-biphenyl-2-carboxyl)- 4-(glutamic acid dimethyl ester amino)cytidine); 0 (Antiviral Agents); 0 (Benzodioxoles); 0 (DNA, Viral); 0 (Hepatitis B Surface Antigens); 0 (Hepatitis B e Antigens); 5CSZ8459RP (Cytidine); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:141121
[Lr] Data última revisão:
141121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141121
[St] Status:MEDLINE



página 1 de 23 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde