Base de dados : MEDLINE
Pesquisa : C02.782 [Categoria DeCS]
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[PMID]:28456056
[Au] Autor:Poirier EZ; Vignuzzi M
[Ad] Endereço:Institut Pasteur, Centre National de la Recherche Scientifique, UMR 3569, Paris, France; University of Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France.
[Ti] Título:Virus population dynamics during infection.
[So] Source:Curr Opin Virol;23:82-87, 2017 04.
[Is] ISSN:1879-6265
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:During RNA virus infection of a host, error-prone viral replication will give rise to a cloud of genetically-linked mutants, as well as truncated, defective genomes. In this review, we describe the dynamics of viral diversity during infection, illustrating that the viral population fluctuates greatly in number of genomes and composition of mutants, in relation with the existence of physical barriers or immune pressures. We illustrate the importance of generating diversity by analyzing the case of fidelity variants, largely attenuated in vivo. Recombination is also considered in its various roles: redistribution of mutations on full-length genomes, and production of highly-immunostimulatory defective genomes. We cover these notions by underlining, when they exist, the differences between acute and persistent infections.
[Mh] Termos MeSH primário: Variação Genética
Dinâmica Populacional
Infecções por Vírus de RNA/virologia
Vírus/crescimento & desenvolvimento
Vírus/genética
[Mh] Termos MeSH secundário: Genética Populacional
Vírus/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


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[PMID]:29111455
[Au] Autor:Shi M; Zhang YZ; Holmes EC
[Ad] Endereço:Marie Bashir Institute for Infectious Diseases and Biosecurity, Charles Perkins Centre, School of Life and Environmental Sciences and Sydney Medical School, The University of Sydney, Sydney, Australia; State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Department of Zoonoses, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, China.
[Ti] Título:Meta-transcriptomics and the evolutionary biology of RNA viruses.
[So] Source:Virus Res;243:83-90, 2018 01 02.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Metagenomics is transforming the study of virus evolution, allowing the full assemblage of virus genomes within a host sample to be determined rapidly and cheaply. The genomic analysis of complete transcriptomes, so-called meta-transcriptomics, is providing a particularly rich source of data on the global diversity of RNA viruses and their evolutionary history. Herein we review some of the insights that meta-transcriptomics has provided on the fundamental patterns and processes of virus evolution, with a focus on the recent discovery of a multitude of novel invertebrate viruses. In particular, meta-transcriptomics shows that the RNA virus world is more fluid than previously realized, with relatively frequent changes in genome length and structure. As well as having a transformative impact on studies of virus evolution, meta-transcriptomics presents major new challenges for virus classification, with the greater sampling of host taxa now filling many of the gaps on virus phylogenies that were previously used to define taxonomic groups. Given that most viruses in the future will likely be characterized using metagenomics approaches, and that we have evidently only sampled a tiny fraction of the total virosphere, we suggest that proposals for virus classification pay careful attention to the wonders unearthed in this new age of virus discovery.
[Mh] Termos MeSH primário: Evolução Molecular
Infecções por Vírus de RNA/virologia
Vírus de RNA/genética
[Mh] Termos MeSH secundário: Animais
Genoma Viral
Seres Humanos
Metagenômica
Filogenia
Vírus de RNA/classificação
Vírus de RNA/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171108
[St] Status:MEDLINE


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[PMID]:29050792
[Au] Autor:Sakuna K; Elliman J; Owens L
[Ad] Endereço:College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, 4811, Australia; Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, 80240, Thailand.
[Ti] Título:Comparison of molecular detection PCR methods for chequa iflavirus in freshwater crayfish, Cherax quadricarinatus.
[So] Source:J Virol Methods;251:139-144, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Chequa iflavirus (+ve sense ssRNA virus) infects redclaw crayfish (Cherax quadricarinatus) and it may cause mortality reaching 20-40% after about three weeks following stress. The sequence of the RNA-dependent RNA polymerase at nucleotide position 8383-9873 was used for developing and comparing PCR-based detection protocols. The reverse transcription, quantitative, polymerase chain reaction (RT-qPCR) was specific against nine Picornavirales and crustacean viruses and its' measurement of uncertainty (0.07-1.37) was similar to PCRs for other crustacean viruses. In vitro, the reverse transcription loop-mediated isothermal amplification (RT-LAMP) read at 60min had poor repeatability for a linearized plasmid with an iflavirus insert when compared with RT-PCR visualised on an electrophoretic gel and RT-qPCR; both sensitive to 10 copies. In a limited, comparative sample of clinical crayfish haemolymph, the lowest, non-zero copies were 2.88×10 for RT-PCR and 4.60×10 for the RT-qPCR. In 68 further clinical crayfish haemolymph samples tested by RT-qPCR only, copy numbers ranged from 0 to 1.14×10 . For RT-qPCR, the amplification plots, melt curves and the C values indicated that the C above 34.0 is a potential negative result but examination of the melt curve is necessary for an accurate interpretation. A suggested program of testing for crayfish farmers would consist of non-destructive bleeding, labelling of crayfish and screening with RT-qPCR. Only those crayfish nominally negative (below detectable limits) would be used for broodstock or selective breeding.
[Mh] Termos MeSH primário: Astacoidea/virologia
Técnicas de Diagnóstico Molecular/métodos
Técnicas de Amplificação de Ácido Nucleico/métodos
Infecções por Vírus de RNA/veterinária
Vírus de RNA/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Água Doce
Infecções por Vírus de RNA/virologia
Vírus de RNA/genética
[Pt] Tipo de publicação:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171021
[St] Status:MEDLINE


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[PMID]:28962967
[Au] Autor:Gao F; Jiang JZ; Wang JY; Wei HY
[Ad] Endereço:Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China; Shanghai Ocean University, Shanghai, 201306, China. Electronic address: tianchengyinuo@163.com.
[Ti] Título:Real-time isothermal detection of Abalone herpes-like virus and red-spotted grouper nervous necrosis virus using recombinase polymerase amplification.
[So] Source:J Virol Methods;251:92-98, 2018 Jan.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Abalone herpes-like virus (AbHV) and Red-spotted grouper nervous necrosis virus (RGNNV) are two serious viruses that infect animal populations in aquaculture. Both viruses cause diseases associated with high mortality rates, resulting in dramatic economic losses in the aquaculture industry. There are currently no effective treatments for either of these two viral diseases. Thus, early, rapid, and accurate diagnosis plays a fundamental role in disease prevention and control in aquaculture. Traditional methods of diagnosis, such as virus culture, enzyme-linked immunoassay, and polymerase chain reaction (PCR), are either time consuming or require sophisticated temperature control devices. In this study, one sets of specific primers and probes were designed for the real-time quantitative recombinase polymerase amplification (qRPA) detection of AbHV and RGNNV separately. The sensitivity and specificity of detection were evaluated by comparison with detection by conventional PCR and quantitative PCR. The optimal reaction temperature and time for virus detection is 37°C for 20min. The detection limit is 100 copies per reaction, making this approach faster and more sensitive than qPCR in this study. In a field application, the detection percentage of qRPA was higher than that of qPCR for both AbHV and NNV. Additionally, good correlation was found between qRPA and qPCR detection (R >0.8). The methods presented here can be used as alternatives to qPCR for quick and quantitative detection of pathogens infecting aquaculture species.
[Mh] Termos MeSH primário: Infecções por Vírus de DNA/veterinária
Herpesviridae/isolamento & purificação
Técnicas de Diagnóstico Molecular/métodos
Nodaviridae/isolamento & purificação
Técnicas de Amplificação de Ácido Nucleico/métodos
Infecções por Vírus de RNA/veterinária
[Mh] Termos MeSH secundário: Animais
Aquicultura
Infecções por Vírus de DNA/diagnóstico
Peixes
Gastrópodes
Herpesviridae/genética
Nodaviridae/genética
Infecções por Vírus de RNA/diagnóstico
Sensibilidade e Especificidade
Medicina Veterinária/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171001
[St] Status:MEDLINE


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[PMID]:27773730
[Au] Autor:Wang TY; Chen YM; Chen TY
[Ad] Endereço:Laboratory of Molecular Genetics, Institute of Biotechnology, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan; Translational Center for Marine Biotechnology, National Cheng Kung University, Tainan 70101, Taiwan.
[Ti] Título:Molecular cloning of orange-spotted grouper (Epinephelus coioides) heat shock transcription factor 1 isoforms and characterization of their expressions in response to nodavirus.
[So] Source:Fish Shellfish Immunol;59:123-136, 2016 Dec.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Heat shock transcription factor 1 (HSF1) regulates heat shock proteins (HSPs), which assist in protein folding and inhibit protein denaturation following stress. HSF1 was firstly cloned from orange-spotted grouper and exists as two isoforms, one with (osgHSF1a) and one without (osgHSF1b) exon 11. Heat exposure increased the expression of osgHSF1b while cold exposure increased that of osgHSF1a. Both isoforms were mainly expressed in the brains, eyes, and fins. Expression of osgHSF1b was higher than osgHSF1a during development. Poly I:C and LPS could also induce osgHSF1 isoforms expression differentially. Exposure to nervous necrosis virus (NNV) increased the level of both osgHSF1 isoforms at 12 h. GF-1 cells with overexpression of osgHSF1 isoforms enhanced viral loads within 24 h, whereas both pharmacological inhibition and RNA interference of HSF1 reduced virus infection. This study shows that osgHSF1 can support the early stage of virus infection and provides a new insight into the molecular regulation of osgHSF1 between the influence of temperatures and immunity.
[Mh] Termos MeSH primário: Bass
Proteínas de Ligação a DNA/genética
Doenças dos Peixes/imunologia
Proteínas de Peixes/genética
Regulação da Expressão Gênica/imunologia
Infecções por Vírus de RNA/veterinária
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Sequência de Bases
Clonagem Molecular
DNA Complementar/genética
DNA Complementar/metabolismo
Proteínas de Ligação a DNA/química
Proteínas de Ligação a DNA/metabolismo
Proteínas de Peixes/química
Proteínas de Peixes/metabolismo
Fatores de Transcrição de Choque Térmico
Temperatura Alta/efeitos adversos
Imunidade/efeitos dos fármacos
Lipopolissacarídeos/farmacologia
Nodaviridae/fisiologia
Filogenia
Poli I-C/farmacologia
Isoformas de Proteínas/química
Isoformas de Proteínas/genética
Isoformas de Proteínas/metabolismo
Infecções por Vírus de RNA/imunologia
Alinhamento de Sequência/veterinária
Fatores de Transcrição/química
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (DNA-Binding Proteins); 0 (Fish Proteins); 0 (Heat Shock Transcription Factors); 0 (Lipopolysaccharides); 0 (Protein Isoforms); 0 (Transcription Factors); O84C90HH2L (Poly I-C)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171203
[Lr] Data última revisão:
171203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


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[PMID]:28954889
[Au] Autor:Ge M; Luo Z; Qiao Z; Zhou Y; Cheng X; Geng Q; Cai Y; Wan P; Xiong Y; Liu F; Wu K; Liu Y; Wu J
[Ad] Endereço:State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China; and.
[Ti] Título:HERP Binds TBK1 To Activate Innate Immunity and Repress Virus Replication in Response to Endoplasmic Reticulum Stress.
[So] Source:J Immunol;199(9):3280-3292, 2017 Nov 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Host innate immunity is crucial for cellular responses against viral infection sensed by distinct pattern recognition receptors and endoplasmic reticulum (ER) stress. Enterovirus 71 (EV71) is a causative agent of hand, foot, and mouth disease and neurological diseases. However, the exact mechanism underlying the link between ER stress induced by EV71 infection and host innate immunity is largely unknown. In this study, we demonstrated that EV71 infection induces the homocysteine-induced ER protein (HERP), a modulator of the ER stress response which is dependent on the participation of MAVS. Virus-induced HERP subsequently stimulates host innate immunity to repress viral replication by promoting type-I IFNs (IFN-α and IFN-ß) and type-III IFN (IFN-λ1) expression. Through interacting with TANK-binding kinase 1, HERP amplifies the MAVS signaling and facilitates the phosphorylation and nuclear translocation of IFN regulatory factor 3 and NF-κB to enhance the expression of IFNs, which leads to a broad inhibition of the replication of RNA viruses, including EV71, Sendai virus, influenza A virus, and vesicular stomatitis virus. Therefore, we demonstrated that HERP plays an important role in the regulation of host innate immunity in response to ER stress during the infection of RNA viruses. These findings provide new insights into the mechanism underlying the replication of RNA viruses and the production of IFNs, and also demonstrate a new role of HERP in the regulation of host innate immunity in response to viral infection.
[Mh] Termos MeSH primário: Estresse do Retículo Endoplasmático/imunologia
Imunidade Inata
Proteínas de Membrana/imunologia
Proteínas Serina-Treonina Quinases/imunologia
Infecções por Vírus de RNA/imunologia
Vírus de RNA/fisiologia
Replicação Viral/imunologia
[Mh] Termos MeSH secundário: Animais
Estresse do Retículo Endoplasmático/genética
Feminino
Seres Humanos
Fator Regulador 3 de Interferon/genética
Fator Regulador 3 de Interferon/imunologia
Interferons/genética
Interferons/imunologia
Masculino
Proteínas de Membrana/genética
Camundongos
Camundongos Endogâmicos BALB C
Proteínas Serina-Treonina Quinases/genética
Infecções por Vírus de RNA/genética
Infecções por Vírus de RNA/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HERPUD1 protein, human); 0 (Herpud1 protein, mouse); 0 (IRF3 protein, human); 0 (Interferon Regulatory Factor-3); 0 (Irf3 protein, mouse); 0 (Membrane Proteins); 9008-11-1 (Interferons); EC 2.7.1.- (Tbk1 protein, mouse); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (TBK1 protein, human)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171026
[Lr] Data última revisão:
171026
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170929
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1700376


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[PMID]:28934431
[Au] Autor:Moni MA; Lio' P
[Ad] Endereço:Garvan Institute of Medical Research, University of New South Wales, Sydney,Australia.
[Ti] Título:Genetic Profiling and Comorbidities of Zika Infection.
[So] Source:J Infect Dis;216(6):703-712, 2017 Sep 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: The difficulty in distinguishing infection by Zika virus (ZIKV) from other flaviviruses is a global health concern, particularly given the high risk of neurologic complications (including Guillain-Barré syndrome [GBS]) with ZIKV infection. Methods: We developed quantitative frameworks to compare and explore infectome, diseasome, and comorbidity of ZIKV infections. We analyzed gene expression microarray and RNA-Seq data from ZIKV, West Nile fever (WNF), chikungunya, dengue, yellow fever, Japanese encephalitis virus, GBS, and control datasets. Using neighborhood-based benchmarking and multilayer network topology, we constructed relationship networks based on the Online Mendelian Inheritance in Man database and our identified significant genes. Results: ZIKV infections showed dysregulation in expression of 929 genes. Forty-seven genes were highly expressed in both ZIKV and dengue infections. However, ZIKV shared <15 significant transcripts with other flavivirus infections. Notably, dysregulation of MAFB and SELENBP1 was common to ZIKV, dengue, and GBS infection; ATF5, TNFAIP3, and BAMB1 were common to ZIKV, dengue, and WNF; and NAMPT and PMAlP1 were common to ZIKV, GBS, and WNF. Phylogenetic, ontologic, and pathway analyses showed that ZIKV infection most resembles dengue fever. Conclusions: We have developed methodologies to investigate disease mechanisms and predictions for infectome, diseasome, and comorbidities quantitatively, and identified particular similarities between ZIKV and dengue infections.
[Mh] Termos MeSH primário: Comorbidade
Perfilação da Expressão Gênica
Interações Hospedeiro-Patógeno/genética
Infecções por Vírus de RNA/diagnóstico
Infecção pelo Zika virus/diagnóstico
Zika virus/isolamento & purificação
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica
Ontologia Genética
Seres Humanos
Filogenia
Infecções por Vírus de RNA/classificação
Vírus de RNA/classificação
Vírus de RNA/genética
Vírus de RNA/isolamento & purificação
Análise de Sequência de RNA
Zika virus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix327


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[PMID]:28915264
[Au] Autor:Baltes A; Akpinar F; Inankur B; Yin J
[Ad] Endereço:Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
[Ti] Título:Inhibition of infection spread by co-transmitted defective interfering particles.
[So] Source:PLoS One;12(9):e0184029, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Although virus release from host cells and tissues propels the spread of many infectious diseases, most virus particles are not infectious; many are defective, lacking essential genetic information needed for replication. When defective and viable particles enter the same cell, the defective particles can multiply while interfering with viable particle production. Defective interfering particles (DIPs) occur in nature, but their role in disease pathogenesis and spread is not known. Here, we engineered an RNA virus and its DIPs to express different fluorescent reporters, and we observed how DIPs impact viral gene expression and infection spread. Across thousands of host cells, co-infected with infectious virus and DIPs, gene expression was highly variable, but average levels of viral reporter expression fell at higher DIP doses. In cell populations spatial patterns of infection spread provided the first direct evidence for the co-transmission of DIPs with infectious virus. Patterns of spread were highly sensitive to the behavior of initial or early co-infected cells, with slower overall spread stemming from higher early DIP doses. Under such conditions striking patterns of patchy gene expression reflected localized regions of DIP or virus enrichment. From a broader perspective, these results suggest DIPs contribute to the ecological and evolutionary persistence of viruses in nature.
[Mh] Termos MeSH primário: Vírus Defeituosos/metabolismo
Regulação Viral da Expressão Gênica
Modelos Biológicos
Infecções por Vírus de RNA/metabolismo
Infecções por Vírus de RNA/transmissão
Vírus de RNA/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Cricetinae
Vírus Defeituosos/patogenicidade
Vírus de RNA/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184029


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[PMID]:28869001
[Au] Autor:Brocard M; Ruggieri A; Locker N
[Ad] Endereço:1​Faculty of Health and Medical Sciences, School of Biosciences and Medicine, University of Surrey, Guildford, UK.
[Ti] Título:m6A RNA methylation, a new hallmark in virus-host interactions.
[So] Source:J Gen Virol;98(9):2207-2214, 2017 Sep.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The role of m6A methylation of RNA has remained elusive for decades, but recent technological advances are now allowing the mapping of the m6A methylation landscape at nucleotide level. This has spurred an explosion in our understanding of the role played by RNA epigenetics in RNA biology. m6A modifications have been tied to almost every aspect of the mRNA life cycle and it is now clear that RNA virus genomes are subject to m6A methylation. These modifications play various roles in the viral replication cycle. This review will summarize recent breakthroughs concerning m6A RNA modification and their implications for cellular and viral RNAs.
[Mh] Termos MeSH primário: Infecções por Vírus de RNA/virologia
Vírus de RNA/metabolismo
RNA Mensageiro/metabolismo
RNA Viral/metabolismo
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Metilação
Vírus de RNA/genética
RNA Mensageiro/genética
RNA Viral/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (RNA, Messenger); 0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170905
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000910


  10 / 848 MEDLINE  
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[PMID]:28646651
[Au] Autor:Chrzastek K; Lee DH; Smith D; Sharma P; Suarez DL; Pantin-Jackwood M; Kapczynski DR
[Ad] Endereço:US National Poultry Research Center, Agricultural Research Service, US Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA.
[Ti] Título:Use of Sequence-Independent, Single-Primer-Amplification (SISPA) for rapid detection, identification, and characterization of avian RNA viruses.
[So] Source:Virology;509:159-166, 2017 Sep.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Current technologies with next generation sequencing have revolutionized metagenomics analysis of clinical samples. To achieve the non-selective amplification and recovery of low abundance genetic sequences, a simplified Sequence-Independent, Single-Primer Amplification (SISPA) technique in combination with MiSeq platform was applied to target negative- and positive-sense single-stranded RNA viral sequences. This method allowed successful sequence assembly of full or near full length avian influenza virus (AIV), infectious bronchitis virus (IBV), and Newcastle disease virus (NDV) viral genome. Moreover, SISPA analysis applied to unknown clinical cases of mixed viral infections produced genome assemblies comprising 98% NDV and 99% of IBV genomes. Complete or near complete virus genome sequence was obtained with titers at or above 10 EID /ml (50% embryo infectious dose), and virus identification could be detected with titers at or above 10 EID /ml. Taken together, these studies demonstrate a simple template enrichment protocol for rapid detection and accurate characterization of avian RNA viruses.
[Mh] Termos MeSH primário: Primers do DNA/genética
Vírus da Bronquite Infecciosa/isolamento & purificação
Vírus da Influenza A/isolamento & purificação
Metagenômica/métodos
Vírus da Doença de Newcastle/isolamento & purificação
Técnicas de Amplificação de Ácido Nucleico/métodos
Infecções por Vírus de RNA/veterinária
[Mh] Termos MeSH secundário: Animais
Vírus da Bronquite Infecciosa/genética
Vírus da Influenza A/genética
Vírus da Doença de Newcastle/genética
Aves Domésticas
Doenças das Aves Domésticas/virologia
Infecções por Vírus de RNA/virologia
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170721
[Lr] Data última revisão:
170721
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170625
[St] Status:MEDLINE



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