Base de dados : MEDLINE
Pesquisa : C02.782.123 [Categoria DeCS]
Referências encontradas : 930 [refinar]
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[PMID]:28743463
[Au] Autor:Rivas-Aravena A; Muñoz P; Jorquera P; Diaz A; Reinoso C; González-Catrilelbún S; Sandino AM
[Ad] Endereço:Comisión Chilena de Energía Nuclear, Departamento de Aplicaciones Nucleares, Laboratorio de Radiobiología Celular y Molecular. Nueva Bilbao 12501, Las Condes, Santiago, Chile; Universidad San Sebastián, Facultad de Ciencias, Lota 2465, Providencia, Santiago, Chile. Electronic address: andrea.rivas@c
[Ti] Título:Study of RNA-A Initiation Translation of The Infectious Pancreatic Necrosis Virus.
[So] Source:Virus Res;240:121-129, 2017 08 15.
[Is] ISSN:1872-7492
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The infectious pancreatic necrosis virus (IPNV) is a salmonid pathogen that causes significant economic losses to the aquaculture industry. IPNV is a non-enveloped virus containing two uncapped and non-polyadenylated double strand RNA genomic segments, RNA-A and RNA-B. The viral protein Vpg is covalently attached to the 5' end of both segments. There is little knowledge about its viral cycle, particularly about the translation of the RNAs. Through experiments using mono and bicistronic reporters, in this work we show that the 120-nucleotide-long 5'-UTR of RNA-A contains an internal ribosome entry site (IRES) that functions efficiently both in vitro and in salmon cells. IRES activity is strongly dependent on temperature. Also, the IRES structure is confined to the 5'UTR and is not affected by the viral coding sequence. This is the first report of IRES activity in a fish virus and can give us tools to generate antivirals to attack the virus without affecting fish directly.
[Mh] Termos MeSH primário: Infecções por Birnaviridae/veterinária
Doenças dos Peixes/virologia
Vírus da Necrose Pancreática Infecciosa/genética
Biossíntese de Proteínas
RNA Viral/genética
[Mh] Termos MeSH secundário: Regiões 5' não Traduzidas
Animais
Infecções por Birnaviridae/virologia
Regulação Viral da Expressão Gênica
Vírus da Necrose Pancreática Infecciosa/química
Vírus da Necrose Pancreática Infecciosa/metabolismo
Sítios Internos de Entrada Ribossomal
Conformação de Ácido Nucleico
RNA Mensageiro/química
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
RNA Viral/química
RNA Viral/metabolismo
Ribossomos/genética
Ribossomos/metabolismo
Salmo salar
Proteínas Virais/genética
Proteínas Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (Internal Ribosome Entry Sites); 0 (RNA, Messenger); 0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180209
[Lr] Data última revisão:
180209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE


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[PMID]:28921008
[Au] Autor:Shehata AA; Sultan H; Halami MY; Talaat S; Vahlenkamp TW
[Ad] Endereço:Faculty of Veterinary Medicine, Center for Infectious Diseases, Institute of Virology, Leipzig University, An den Tierkliniken 29, 04103, Leipzig, Germany. dr_awadali_1@yahoo.com.
[Ti] Título:Molecular characterization of very virulent infectious bursal disease virus strains circulating in Egypt from 2003 to 2014.
[So] Source:Arch Virol;162(12):3803-3815, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:In the present study, four very virulent infectious bursal disease virus (vvIBDV) isolates from flocks of chickens with vaccination failure in Egypt in 2003, 2007, 2010 and 2014 were characterized. The four viruses, designated USC2003, USC2007, USC2010 and USC2014, were detected by reverse transcription PCR, subjected to sequencing of both genomic segments (A and B) and compared with geographically and phylogenetically diverse IBDV strains. Phylogenetic analysis of segment A (complete) and B (partial) revealed a close relationship between Egyptian and vvIBDV reference strains of European and Asian origin. The sequences of segments of A and B the current Egyptian isolates were 96.1-98.2% and 96.5-98.7% identical, respectively, to those of other known vvIBDV isolates. The deduced amino acid sequences of VP1, polyprotein (pVP2-VP4-VP3) and VP5 revealed the presence of putative virulence determinants of Egyptian isolates compared with vvIBDV and less virulent (classical and variant) strains. The Egyptian isolates also possess unique amino acids substitutions within the hypervariable region of VP2 that differ from those of other reference IBDV strains. Further studies may be necessary to determine the pathogenic significance of these amino acid substitutions to fully understand the molecular epidemiology and evolution of IBDV.
[Mh] Termos MeSH primário: Infecções por Birnaviridae/veterinária
Variação Genética
Vírus da Doença Infecciosa da Bursa/genética
Vírus da Doença Infecciosa da Bursa/isolamento & purificação
Doenças das Aves Domésticas/patologia
Doenças das Aves Domésticas/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Birnaviridae/epidemiologia
Infecções por Birnaviridae/patologia
Infecções por Birnaviridae/virologia
Galinhas
Egito/epidemiologia
Vírus da Doença Infecciosa da Bursa/classificação
Epidemiologia Molecular
Filogenia
Doenças das Aves Domésticas/epidemiologia
RNA Viral/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Análise de Sequência de DNA
Homologia de Sequência
Proteínas Virais/genética
Virulência
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); 0 (Viral Proteins); 0 (Virulence Factors)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3554-3


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[PMID]:28825213
[Au] Autor:Michel LO; Jackwood DJ
[Ad] Endereço:Food Animal Health Research Program, The Ohio State University/Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH, 44691, USA.
[Ti] Título:Classification of infectious bursal disease virus into genogroups.
[So] Source:Arch Virol;162(12):3661-3670, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Infectious bursal disease virus (IBDV) causes infectious bursal disease (IBD), an immunosuppressive disease of poultry. The current classification scheme of IBDV is confusing because it is based on antigenic types (variant and classical) as well as pathotypes. Many of the amino acid changes differentiating these various classifications are found in a hypervariable region of the capsid protein VP2 (hvVP2), the major host protective antigen. Data from this study were used to propose a new classification scheme for IBDV based solely on genogroups identified from phylogenetic analysis of the hvVP2 of strains worldwide. Seven major genogroups were identified, some of which are geographically restricted and others that have global dispersion, such as genogroup 1. Genogroup 2 viruses are predominately distributed in North America, while genogroup 3 viruses are most often identified on other continents. Additionally, we have identified a population of genogroup 3 vvIBDV isolates that have an amino acid change from alanine to threonine at position 222 while maintaining other residues conserved in this genogroup (I242, I256 and I294). A222T is an important mutation because amino acid 222 is located in the first of four surface loops of hvVP2. A similar shift from proline to threonine at 222 is believed to play a role in the significant antigenic change of the genogroup 2 IBDV strains, suggesting that antigenic drift may be occurring in genogroup 3, possibly in response to antigenic pressure from vaccination.
[Mh] Termos MeSH primário: Infecções por Birnaviridae/veterinária
Genótipo
Vírus da Doença Infecciosa da Bursa/classificação
Vírus da Doença Infecciosa da Bursa/genética
Doenças das Aves Domésticas/virologia
Proteínas Estruturais Virais/genética
[Mh] Termos MeSH secundário: Animais
Infecções por Birnaviridae/virologia
Saúde Global
Filogeografia
Aves Domésticas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (VP2 protein, infectious bursal disease virus); 0 (Viral Structural Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170822
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3500-4


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[PMID]:28795226
[Au] Autor:Holopainen R; Eriksson-Kallio AM; Gadd T
[Ad] Endereço:Finnish Food Safety Authority Evira, Research and Laboratory Services Department, Virology Research Unit, Mustialankatu 3, 00790, Helsinki, Finland. riikka.holopainen@evira.fi.
[Ti] Título:Molecular characterisation of infectious pancreatic necrosis viruses isolated from farmed fish in Finland.
[So] Source:Arch Virol;162(11):3459-3471, 2017 Nov.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Infectious pancreatic necrosis virus (IPNV) has been isolated annually since 1987 from salmonids without clinical signs at coastal fish farms in Finland. In the inland area, viral isolations were rare until 2012, when IPNV was detected at several freshwater fish farms. Between 2013 and 2015, the infection spread and IPNV was continuously isolated from several farms, both inland and on the coast. The aim of this study was to genetically characterise the IPNV isolates collected from Finnish coastal and inland fish farms over the last 15 years, and to detect genetic changes that may have occurred in the virus populations during the study period. The partial VP2 gene sequence from 88 isolates was analysed. In addition, a complete genomic coding sequence was obtained from 11 isolates. Based on the genetic analyses, Finnish IPNV isolates belong to three genogroups: 2, 5 and 6. The genetic properties of the isolates appear to vary between inland farms producing juveniles and food fish farms in the coastal region: the inland farms harboured genogroup 2 isolates, whereas at coastal farms, all three genogroups were detected. Little genetic variation was observed within the Finnish genogroup 2 and 5 isolates, whereas among the genogroup 6 isolates, two subgroups were detected. All isolates studied demonstrated amino acid patterns in the viral VP2 gene previously associated with avirulence. However, increased mortality was detected at some of the farms, indicating that more research is needed to clarify the relationship between the pathogenicity and genetic properties of IPNV isolates from different genogroups.
[Mh] Termos MeSH primário: Infecções por Birnaviridae/veterinária
Doenças dos Peixes/virologia
Vírus da Necrose Pancreática Infecciosa
Salmonidae
[Mh] Termos MeSH secundário: Animais
Aquicultura
Infecções por Birnaviridae/epidemiologia
Infecções por Birnaviridae/virologia
Finlândia/epidemiologia
Regulação Viral da Expressão Gênica
Genoma Viral
Vírus da Necrose Pancreática Infecciosa/genética
Filogenia
Proteínas Estruturais Virais/genética
Proteínas Estruturais Virais/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (VP2 protein, infectious pancreatic necrosis virus); 0 (Viral Structural Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3525-8


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[PMID]:28662410
[Au] Autor:Esmailnejad A; Nikbakht Brujeni G; Badavam M
[Ad] Endereço:Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
[Ti] Título:LEI0258 microsatellite variability and its association with humoral and cell mediated immune responses in broiler chickens.
[So] Source:Mol Immunol;90:22-26, 2017 Oct.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Major histocompatibility complex (MHC) has a profound influence on disease resistance or susceptibility, productivity and important economic traits in chicken. Association of the MHC with a wide range of immune responses makes it a valuable predictive factor for the disease pathogenesis and outcome. The tandem repeat LEI0258 is a genetic marker which is located within the B locus of chicken MHC and strongly associated with serologically defined haplotypes. LEI0258 microsatellite marker was applied to investigate the MHC polymorphism in Ross 308 broiler chicken (N=104). Association of LEI0258 alleles with humoral and cell mediated immune responses to Newcastle disease (ND), Infectious bursal disease (IBD) and Avian influenza (AI) vaccines were also examined. LEI0258 polymorphism was determined by PCR-based fragment analysis, and association of LEI0258 alleles with immune responses were evaluated using multivariate regression analysis and GLM procedures. A total of seven alleles ranging from 195 to 448bp were found, including two novel alleles (263 and 362bp) that were unique in Ross 308 broiler population. Association study revealed a significant influence of MHC alleles on humoral and cellular immune responses in Ross population (P<0.05). Alleles 385 and 448bp were associated with increased peripheral blood lymphocyte proliferation response. Alleles 300, 362 and 448bp had a positive effect on immune responses to Infectious bursal disease vaccine, and allele 263bp was significantly correlated with elevated antibody titer against Newcastle disease vaccine. Results obtained from this study confirmed the important role of MHC as a candidate gene marker for immune responses that could be used in genetic improvement of disease-resistant traits and resource conservation in broiler population.
[Mh] Termos MeSH primário: Infecções por Birnaviridae/imunologia
Galinhas/imunologia
Imunidade Celular/genética
Imunidade Humoral/genética
Influenza Aviária/imunologia
Repetições de Microssatélites/genética
Doença de Newcastle/imunologia
[Mh] Termos MeSH secundário: Alelos
Animais
Infecções por Birnaviridae/veterinária
Infecções por Birnaviridae/virologia
Proliferação Celular
Galinhas/genética
Marcadores Genéticos/genética
Vacinas contra Influenza/imunologia
Influenza Aviária/virologia
Complexo Principal de Histocompatibilidade/genética
Complexo Principal de Histocompatibilidade/imunologia
Doença de Newcastle/virologia
Polimorfismo Genético/genética
Vacinas Virais/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); 0 (Influenza Vaccines); 0 (Newcastle disease virus vaccine MTH-68-H); 0 (Viral Vaccines)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170630
[St] Status:MEDLINE


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[PMID]:28523925
[Au] Autor:Norouzian H; Farjanikish G; Hosseini H
[Ti] Título:Genetic and pathologic characteristics of infectious bursal disease viruses isolated from broiler chickens in Iran during 2014-2015.
[So] Source:Acta Virol;61(2):191-196, 2017.
[Is] ISSN:0001-723X
[Cp] País de publicação:Slovakia
[La] Idioma:eng
[Ab] Resumo:Infectious bursal disease (IBD) virus causes a highly contagious immunosuppressive disease in chickens. A total number of 12 pooled bursal samples were collected during 2014-2015 from broiler farms in different regions of Iran. Typical macroscopical and histopathological lesions of the bursa of Fabricius were found similar to reports by other researchers. A 474-bp part of hypervariable region of VP2 (hvVP2) was sequenced and analyzed. Ten isolates had the characteristic amino acid residues of very virulent IBD (vvIBD) viruses and the other two were identified as attenuated (vaccine) strains. The vvIBD isolates had a unique G to S mutation at position 254, compared to other Iranian vvIBD isolates. Two attenuated isolates had the mutation 253Q, not found in D78 strain, creating virulent variant of vaccine strains. Degree of similarity among the studied vvIBD isolates was relatively high (97.6-100%), proposing a common ancestor for them. However, they were partly different from previous Iranian and neighbor countries' isolates (96.2-97.3% similarity to Shiraz isolate and 95.7-96.7% to Iraq and Turkey isolates). In phylogenetic analysis, the studied vvIBD isolates classified as a separate subgroup in the group of isolates from Iran and neighbor countries. More studies on genetic and antigenic characteristics of these isolates as well as probable modifications in their pathogenicity are needed to evaluate the significance of the mentioned differences.
[Mh] Termos MeSH primário: Infecções por Birnaviridae/veterinária
Galinhas
Vírus da Doença Infecciosa da Bursa/genética
[Mh] Termos MeSH secundário: Animais
Infecções por Birnaviridae/epidemiologia
Infecções por Birnaviridae/virologia
Vírus da Doença Infecciosa da Bursa/patogenicidade
Irã (Geográfico)/epidemiologia
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Messenger)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170520
[St] Status:MEDLINE
[do] DOI:10.4149/av_2017_02_09


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[PMID]:28494932
[Au] Autor:Kjærup RB; Juul-Madsen HR; Norup LR; Sørensen P; Dalgaard TS
[Ad] Endereço:Department of Animal Science, Aarhus University, Blichers Allé 20, P.O. Box 50, DK-8830 Tjele, Denmark. Electronic address: rikke.kjaerup@anis.au.dk.
[Ti] Título:Comparison of growth performance and immune parameters of three commercial chicken lines used in organic production.
[So] Source:Vet Immunol Immunopathol;187:69-79, 2017 May.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Owing to the higher demands for avoiding medication and antibiotics, health status of the production animals plays an important role in the poultry industry, especially in organic poultry systems. Immunity plays a major role in keeping the host free from disease, and it is evident that the host's genetic make-up influences immunity and disease resistance/susceptibility in chickens. Previously, breeding strategies aimed at selection for resistance against specific diseases with the risk of creating less disease resistance against other pathogens. Changing breeding strategies towards selection of chickens with a more general and broad disease resistance or robustness may therefore improve the overall health status, animal welfare, and food security in the poultry production. The aim of this study was therefore to compare the immunocompetence of the presumed "robust" Hellevad chickens with two chicken lines widely used in organic production, Bovans Brown (Bovans) and Hisex White (Hisex). The chickens were subjected to a routine vaccination program comprising one parasite and four viral vaccines. The current study indicates that considerable differences in immunocompetence may exist between commercial layer lines used in organic production. The Hellevad chickens were found to have higher body weight at the end of the experiment (17 weeks of age) than the other two lines. Furthermore, Hellevad and Hisex chickens were found to have higher levels of humoral innate immunity with regard to sample to positive ratio of natural antibodies in serum and concentration of mannose-binding lectin in serum as compared to Bovans. Moreover, indications of an inflammatory response were observed in the Bovans at week 5, corresponding to 1 week after vaccination with live infectious bursal disease virus. With regard to adaptive immune parameters such as IgY concentration in blood and infectious bursal disease virus (IBDV)-specific antibody titres, the Hellevad and Hisex chickens had lower levels than the Bovans. How the differences observed in growth and immune parameters in the three chicken lines influence the immune protection against infection needs to be studied further.
[Mh] Termos MeSH primário: Galinhas/crescimento & desenvolvimento
Agricultura Orgânica/métodos
[Mh] Termos MeSH secundário: Animais
Infecções por Birnaviridae/imunologia
Infecções por Birnaviridae/prevenção & controle
Infecções por Birnaviridae/veterinária
Galinhas/imunologia
Infecções por Coronavirus/imunologia
Infecções por Coronavirus/prevenção & controle
Infecções por Coronavirus/veterinária
Feminino
Imunidade Celular
Imunidade Humoral
Vírus da Bronquite Infecciosa/imunologia
Vírus da Doença Infecciosa da Bursa/imunologia
Contagem de Leucócitos/veterinária
Masculino
Doenças das Aves Domésticas/imunologia
Doenças das Aves Domésticas/prevenção & controle
Especificidade da Espécie
Vacinas Virais/farmacologia
Ganho de Peso
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Vaccines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170513
[St] Status:MEDLINE


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[PMID]:28439709
[Au] Autor:Wen CM
[Ad] Endereço:Department of Life Sciences, National University of Kaohsiung, No. 700, Kaohsiung University Road, Nan-Tzu District, Kaohsiung, 81148, Taiwan. wenchiumin@nuk.edu.tw.
[Ti] Título:Complete genome sequence and phylogenetic analyses of an aquabirnavirus isolated from a diseased marbled eel culture in Taiwan.
[So] Source:Arch Virol;162(8):2467-2471, 2017 Aug.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:An aquabirnavirus was isolated from diseased marbled eels (Anguilla marmorata; MEIPNV1310) with gill haemorrhages and associated mortality. Its genome segment sequences were obtained through next-generation sequencing and compared with published aquabirnavirus sequences. The results indicated that the genome sequence of MEIPNV1310 contains segment A (3099 nucleotides) and segment B (2789 nucleotides). Phylogenetic analysis showed that MEIPNV1310 is closely related to the infectious pancreatic necrosis Ab strain within genogroup II. This genome sequence is beneficial for studying the geographic distribution and evolution of aquabirnaviruses.
[Mh] Termos MeSH primário: Aquabirnavirus/genética
Aquabirnavirus/isolamento & purificação
Infecções por Birnaviridae/veterinária
Enguias/virologia
Doenças dos Peixes/virologia
Genoma Viral
[Mh] Termos MeSH secundário: Animais
Aquabirnavirus/classificação
Aquabirnavirus/patogenicidade
Aquicultura
Infecções por Birnaviridae/virologia
Brânquias/patologia
Brânquias/virologia
Hemorragia/veterinária
Hemorragia/virologia
Sequenciamento de Nucleotídeos em Larga Escala
Vírus da Necrose Pancreática Infecciosa/genética
Filogenia
Análise de Sequência de DNA
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3382-5


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[PMID]:28413051
[Au] Autor:Li K; Liu Y; Zhang Y; Gao L; Liu C; Cui H; Qi X; Gao Y; Zhong L; Wang X
[Ad] Endereço:Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China.
[Ti] Título:Protective efficacy of a novel recombinant Marek's disease virus vector vaccine against infectious bursal disease in chickens with or without maternal antibodies.
[So] Source:Vet Immunol Immunopathol;186:55-59, 2017 Apr.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Infectious bursal disease (IBD) causes significant clinical and economic losses to the poultry industry worldwide. Current vaccine programs using live attenuated and inactivated vaccines have numerous drawbacks. As an alternative solution to control IBD, a Marek's disease virus (MDV) vector vaccine (rMDV-VP2) expressing the VP2 gene of infectious bursal disease virus (IBDV) has been developed. In this study, the protective efficacy of rMDV-VP2 was evaluated in a dose-related experiment which showed that a single dose of 1000 PFU was sufficient to fully protect chickens against IBDV infection. Chickens inoculated with lower doses of rMDV-VP2 (250 or 500 PFU) conferred 80 and 90% protection against IBDV. Next, rMDV-VP2 vaccine provided 90% protection against IBDV in commercial layer chickens with maternal antibodies, which was higher than the protective efficacy using the B87 live vaccine of IBDV. Additionally, rMDV-VP2 conferred effective protection against very virulent MDV challenge in chickens (95% for chickens vaccinated with 250 or 500 PFU and 100% for chickens vaccinated with 1000 or 2000 PFU). These results demonstrated that rMDV-VP2 may be a novel bivalent vaccine against IBD and Marek's disease in chickens.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Infecções por Birnaviridae/veterinária
Galinhas
Vírus da Doença Infecciosa da Bursa
Vacinas contra Doença de Marek/administração & dosagem
Doenças das Aves Domésticas/prevenção & controle
Proteínas Estruturais Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Infecções por Birnaviridae/imunologia
Infecções por Birnaviridae/prevenção & controle
Imunidade Materno-Adquirida
Vírus da Doença Infecciosa da Bursa/genética
Vacinas contra Doença de Marek/genética
Vacinas contra Doença de Marek/imunologia
Doenças das Aves Domésticas/imunologia
Vacinas Sintéticas/genética
Vacinas Sintéticas/imunologia
Proteínas Estruturais Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Marek Disease Vaccines); 0 (VP2 protein, infectious bursal disease virus); 0 (Vaccines, Synthetic); 0 (Viral Structural Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE


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[PMID]:28232067
[Au] Autor:Zhu L; Wang X; Wang K; Yang Q; He J; Qin Z; Geng Y; Ouyang P; Huang X
[Ad] Endereço:Department of Basic Veterinary, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
[Ti] Título:Outbreak of infectious pancreatic necrosis virus (IPNV) in farmed rainbow trout in China.
[So] Source:Acta Trop;170:63-69, 2017 Jun.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Infectious pancreatic necrosis virus (IPNV) is a member of the Aquabirnavirus genus, which caused mass mortality (nearly 100%) in farmed rainbow trout (Oncorhynchus mykiss) in aquaculture farms in 2016, China. Major clinical signs included decreased appetite, mucous-like stools, and darkened pigmentation. Pathological changes in moribund fish were observed, such as marked vacuolar degeneration of the pancreatic cells with pyknotic nucleus and decreased zymogen granules, severe hemorrhage in the liver, and tumidness of respiratory epithelium in gills. In addition, the tissue fluid of diseased fish could produce a cytopathic effect (CPE) in RTG-2 cells. The presence of specific 206bp fragments by the reverse transcription-polymerase chain reaction (RT-PCR) using tissue homogenate of diseased fish and supernatant of infected cells revealed that IPNV could be confirmed. The pathogenicity test of cell culture supernatant detected cumulative mortality of 80%, and the clinical symptoms observed in the moribund and dead fish were similar to the naturally infected fish. Furthermore, the sequence analysis of VP2 gene showed that the isolated virus strain belonged to genogroup 1, and 97% homology with the Mexican IPNV isolate was found. To our knowledge, this is the first report on IPNV natural infection in the southwest of China.
[Mh] Termos MeSH primário: Aquicultura
Infecções por Birnaviridae/epidemiologia
Doenças dos Peixes/epidemiologia
Vírus da Necrose Pancreática Infecciosa/isolamento & purificação
Oncorhynchus mykiss/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Birnaviridae/virologia
China/epidemiologia
Surtos de Doenças
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE



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