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[PMID]:29235980
[Au] Autor:Tucakov AK; Yavuz S; Schürmann EM; Mischler M; Klingebeil A; Meyers G
[Ad] Endereço:Institut für Immunologie, Friedrich-Loeffler-Institut, D-17493 Greifswald-Insel Riems, Germany.
[Ti] Título:Restoration of glycoprotein E dimerization via pseudoreversion partially restores virulence of classical swine fever virus.
[So] Source:J Gen Virol;99(1):86-96, 2018 Jan.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The classical swine fever virus (CSFV) represents one of the most important pathogens of swine. The CSFV glycoprotein E is an essential structural protein and an important virulence factor. The latter is dependent on the RNase activity of this envelope protein and, most likely, its secretion from the infected cell. A further important feature with regard to its function as a virulence factor is the formation of disulfide-linked E homodimers that are found in virus-infected cells and virions. Mutant CSFV lacking cysteine (Cys) 171, the residue responsible for intermolecular disulfide bond formation, were found to be attenuated in pigs (Tews BA, Schürmann EM, Meyers G. J Virol 2009;83:4823-4834). In the course of an animal experiment with such a dimerization-negative CSFV mutant, viruses were reisolated from pigs that contained a mutation of serine (Ser) 209 to Cys. This mutation restored the ability to form disulphide-linked E homodimers. In transient expression studies E mutants carrying the S209C change were found to form homodimers with about wt efficiency. Also the secretion level of the mutated proteins was equivalent to that of wt E . Virus mutants containing the Cys171Ser/Ser209Cys configuration exhibited wt growth rates and increased virulence when compared with the Cys171Ser mutant. These results provide further support for the connection between CSFV virulence and E dimerization.
[Mh] Termos MeSH primário: Peste Suína Clássica/virologia
Vírus da Febre Suína Clássica/genética
Vírus da Febre Suína Clássica/patogenicidade
Células Epiteliais/virologia
Mutação
Proteínas do Envelope Viral/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Linhagem Celular
Peste Suína Clássica/patologia
Vírus da Febre Suína Clássica/metabolismo
Cricetulus
Expressão Gênica
Engenharia Genética
Rim/virologia
Multimerização Proteica
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Suínos
Proteínas do Envelope Viral/metabolismo
Carga Viral
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Recombinant Proteins); 0 (Viral Envelope Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000990


  2 / 1464 MEDLINE  
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[PMID]:28797058
[Au] Autor:Herrera-Ibatá DM; Martínez-López B; Quijada D; Burton K; Mur L
[Ad] Endereço:Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, United States of America.
[Ti] Título:Quantitative approach for the risk assessment of African swine fever and Classical swine fever introduction into the United States through legal imports of pigs and swine products.
[So] Source:PLoS One;12(8):e0182850, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The US livestock safety strongly depends on its capacity to prevent the introduction of Transboundary Animal Diseases (TADs). Therefore, accurate and updated information on the location and origin of those potential TADs risks is essential, so preventive measures as market restrictions can be put on place. The objective of the present study was to evaluate the current risk of African swine fever (ASF) and Classical swine fever (CSF) introduction into the US through the legal importations of live pigs and swine products using a quantitative approach that could be later applied to other risks. Four quantitative stochastic risk assessment models were developed to estimate the monthly probabilities of ASF and CSF release into the US, and the exposure of susceptible populations (domestic and feral swine) to these introductions at state level. The results suggest a low annual probability of either ASF or CSF introduction into the US, by any of the analyzed pathways (5.5*10-3). Being the probability of introduction through legal imports of live pigs (1.8*10-3 for ASF, and 2.5*10-3 for CSF) higher than the risk of legally imported swine products (8.90*10-4 for ASF, and 1.56*10-3 for CSF). This could be caused due to the low probability of exposure associated with this type of commodity (products). The risk of feral pigs accessing to swine products discarded in landfills was slightly higher than the potential exposure of domestic pigs through swill feeding. The identification of the months at highest risk, the origin of the higher risk imports, and the location of the US states most vulnerable to those introductions (Iowa, Minnesota and Wisconsin for live swine and California, Florida and Texas for swine products), is valuable information that would help to design prevention, risk-mitigation and early-detection strategies that would help to minimize the catastrophic consequences of potential ASF/CSF introductions into the US.
[Mh] Termos MeSH primário: Febre Suína Africana/prevenção & controle
Peste Suína Clássica/prevenção & controle
Comércio
[Mh] Termos MeSH secundário: Febre Suína Africana/diagnóstico
Animais
Peste Suína Clássica/diagnóstico
Diagnóstico Precoce
Medição de Risco
Fatores de Risco
Suínos
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182850


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[PMID]:28721853
[Au] Autor:Lv H; Dong W; Qian G; Wang J; Li X; Cao Z; Lv Q; Wang C; Guo K; Zhang Y
[Ad] Endereço:1​College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling 712100, Shaanxi, PR China.
[Ti] Título:uS10, a novel Npro-interacting protein, inhibits classical swine fever virus replication.
[So] Source:J Gen Virol;98(7):1679-1692, 2017 Jul.
[Is] ISSN:1465-2099
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Classical swine fever (CSF) is a severe, febrile and highly contagious disease caused by classical swine fever virus (CSFV) that has resulted in huge economic losses in the pig industry worldwide. CSFV Npro has been actively studied but remains incompletely understood. Few studies have investigated the cellular proteins that interact with Npro and their participation in viral replication. Here, the yeast two-hybrid (Y2H) system was employed to screen Npro-interacting proteins from a porcine alveolar macrophage (PAM) cDNA library, and a blast search of the NCBI database revealed that 15 cellular proteins interact with Npro. The interaction of Npro with ribosomal protein S20, also known as universal S10 (uS10), was further confirmed by co-immunoprecipitation and glutathione S-transferase pull-down assays. Furthermore, uS10 overexpression inhibited CSFV replication, whereas the knockdown of uS10 promoted CSFV replication in PAMs. In addition, Npro or CSFV reduced uS10 expression in PAMs in a proteasome-dependent manner, indicating that Npro-uS10 interaction might contribute to persistent CSFV replication. Our previous research showed that CSFV decreases Toll-like receptor 3 (TLR3) expression. The results showed that uS10 knockdown reduced TLR3 expression, and that uS10 overexpression increased TLR3 expression. Notably, uS10 knockdown did not promote CSFV replication following TLR3 overexpression. Conversely, uS10 overexpression did not inhibit CSFV replication following TLR3 knockdown. These results revealed that uS10 inhibits CSFV replication by modulating TLR3 expression. This work addresses a novel aspect of the regulation of the innate antiviral immune response during CSFV infection.
[Mh] Termos MeSH primário: Peste Suína Clássica/metabolismo
Vírus da Febre Suína Clássica/fisiologia
Endopeptidases/metabolismo
Proteínas Ribossômicas/metabolismo
Proteínas Virais/metabolismo
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Peste Suína Clássica/genética
Peste Suína Clássica/virologia
Vírus da Febre Suína Clássica/genética
Endopeptidases/genética
Ligação Proteica
Proteínas Ribossômicas/genética
Suínos
Receptor 3 Toll-Like/genética
Receptor 3 Toll-Like/metabolismo
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ribosomal Proteins); 0 (Toll-Like Receptor 3); 0 (Viral Proteins); 0 (ribosomal protein S20); EC 3.4.- (Endopeptidases); EC 3.4.- (N(pro) protein, swine fever virus)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170720
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000867


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[PMID]:28542321
[Au] Autor:Holinka LG; O'Donnell V; Risatti GR; Azzinaro P; Arzt J; Stenfeldt C; Velazquez-Salinas L; Carlson J; Gladue DP; Borca MV
[Ad] Endereço:Plum Island Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Greenport, New York, United States of America.
[Ti] Título:Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G.
[So] Source:PLoS One;12(5):e0177433, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Prophylactic vaccination using live attenuated classical swine fever (CSF) vaccines has been a very effective method to control the disease in endemic regions and during outbreaks in previously disease-free areas. These vaccines confer effective protection against the disease at early times post-vaccination although the mechanisms mediating the protection are poorly characterized. Here we present the events occurring after the administration of our in-house developed live attenuated marker vaccine, FlagT4Gv. We previously reported that FlagT4Gv intramuscular (IM) administered conferred effective protection against intranasal challenge with virulent CSFV (BICv) as early as 7 days post-vaccination. Here we report that FlagT4Gv is able to induce protection against disease as early as three days post-vaccination. Immunohistochemical testing of tissues from FlagT4Gv-inoculated animals showed that tonsils were colonized by the vaccine virus by day 3 post-inoculation. There was a complete absence of BICv in tonsils of FlagT4Gv-inoculated animals which had been intranasal (IN) challenged with BICv 3 days after FlagT4Gv infection, confirming that FlagT4Gv inoculation confers sterile immunity. Analysis of systemic levels of 19 different cytokines in vaccinated animals demonstrated an increase of IFN-α three days after FlagT4Gv inoculation compared with mock infected controls.
[Mh] Termos MeSH primário: Peste Suína Clássica/imunologia
Peste Suína Clássica/prevenção & controle
Vírus da Febre Suína Clássica/imunologia
Vacinas Virais/farmacologia
[Mh] Termos MeSH secundário: Animais
Peste Suína Clássica/virologia
Vírus da Febre Suína Clássica/patogenicidade
Vírus da Febre Suína Clássica/fisiologia
Citocinas/sangue
Feminino
Interferon-alfa/sangue
Tonsila Palatina/imunologia
Tonsila Palatina/virologia
Sus scrofa
Suínos
Fatores de Tempo
Vacinas Atenuadas/farmacologia
Vacinas Marcadoras/farmacologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Interferon-alpha); 0 (Vaccines, Attenuated); 0 (Vaccines, Marker); 0 (Viral Vaccines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177433


  5 / 1464 MEDLINE  
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[PMID]:28499403
[Au] Autor:Liu Z; Liu Y; Zhang Y; Yang Y; Ren J; Zhang X; Du E
[Ad] Endereço:College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
[Ti] Título:Surface displaying of swine IgG1 Fc enhances baculovirus-vectored vaccine efficacy by facilitating viral complement escape and mammalian cell transduction.
[So] Source:Vet Res;48(1):29, 2017 May 12.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Baculovirus-mediated gene transfer has been developed as a vaccine design strategy against a number of diseases without apparent viral replication. However, it has been hampered by complement-dependent inactivation, thus hindering the in vivo application of baculovirus. A variety of approaches have been exploited to bypass the complement system in the serum. In this study, we constructed and screened a series of baculovirus vectors displaying complement interfering factors, of which a baculovirus vector displaying swine IgG1 Fc (pFc) showed the highest complement antagonism (75.6%). Flow cytometry analysis of transduced cells demonstrated that the baculovirus display of pFc had a significant increase in transduction efficiency and transgene expression of reporter genes. On this basis, a VSV-G-pseudotyped with swine IgG1 Fc surface displayed baculovirus vector was developed to express the classical swine fever virus (CSFV) E2 gene. The translational enhancers Syn21 and P10UTR were incorporated to improve the antigen expression. The E2 gene was efficiently expressed in both insect and mammalian cells. Pigs immunized with this recombinant baculovirus developed high levels of E2-specific antibody, CSFV-specific neutralizing antibody and IFN-γ-secreting cellular immune responses. These results demonstrate that the strategy of surface-displaying swine IgG1 Fc has a great potential to improve the efficiency of baculovirus-vectored vaccine for CSFV and other swine pathogens.
[Mh] Termos MeSH primário: Baculoviridae/imunologia
Peste Suína Clássica/prevenção & controle
Vírus da Febre Suína Clássica/imunologia
Proteínas do Sistema Complemento/imunologia
Imunoglobulina G/imunologia
[Mh] Termos MeSH secundário: Animais
Peste Suína Clássica/imunologia
Citometria de Fluxo/veterinária
Receptores de IgG/imunologia
Suínos/imunologia
Transdução Genética/métodos
Vacinas Sintéticas/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Immunoglobulin G); 0 (Receptors, IgG); 0 (Vaccines, Synthetic); 9007-36-7 (Complement System Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170514
[St] Status:MEDLINE
[do] DOI:10.1186/s13567-017-0434-5


  6 / 1464 MEDLINE  
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[PMID]:28376865
[Au] Autor:Yadav S; Weng HY
[Ad] Endereço:Department of Comparative Pathobiology, Purdue University, 625 Harrison Street, West Lafayette, IN, 47907, USA.
[Ti] Título:Estimating the scale of adverse animal welfare consequences of movement restriction and mitigation strategies in a classical swine fever outbreak.
[So] Source:BMC Vet Res;13(1):83, 2017 Apr 04.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The study aim was to quantify the impact of movement restriction on the well-being of pigs and the associated mitigation responses during a classical swine fever (CSF) outbreak. We developed a stochastic risk assessment model and incorporated Indiana swine industry statistics to estimate the timing and number of swine premises that would encounter overcrowding or feed interruption resulting from movement restriction. Our model also quantified the amount of on-farm euthanasia and movement of pigs to slaughter plants required to alleviate those conditions. We simulated various single-site (i.e., an outbreak initiated from one location) and multiple-site (i.e., an outbreak initiated from more than one location) outbreak scenarios in Indiana to estimate outputs. RESULTS: The study estimated that 14% of the swine premises in Indiana would encounter overcrowding or feed interruption due to movement restriction implemented during a CSF outbreak. The number of premises that would experience animal welfare conditions was about 2.5 fold of the number of infected premises. On-farm euthanasia needed to be performed on 33% of those swine premises to alleviate adverse animal welfare conditions, and more than 90% of on-farm euthanasia had to be carried out within 2 weeks after the implementation of movement restriction. Conversely, movement of pigs to slaughter plants could alleviate 67% of adverse animal welfare conditions due to movement restriction, and only less than 1% of movement of pigs to slaughter plants had to be initiated in the first 2 weeks of movement restrictions. The risk of secondary outbreaks due to movement of pigs from movement restriction areas to slaughter plants was low and only seven pigs from each shipment needed to be tested for CSF infection to prevent a secondary outbreak. CONCLUSIONS: We found that the scale of adverse animal welfare consequences of movement restriction during a CSF outbreak in Indiana was substantial, and controlled movement of pigs to slaughter plants was an efficient and low-risk alternative mitigation response to on-farm euthanasia. The output estimates generated from this study provide empirical evidence for decision makers to properly incorporate required resources for mitigating adverse animal welfare conditions in CSF outbreak management strategic planning.
[Mh] Termos MeSH primário: Bem-Estar do Animal
Peste Suína Clássica/prevenção & controle
Surtos de Doenças/veterinária
[Mh] Termos MeSH secundário: Matadouros
Criação de Animais Domésticos/métodos
Animais
Controle de Doenças Transmissíveis/métodos
Surtos de Doenças/prevenção & controle
Eutanásia Animal
Indiana/epidemiologia
Medição de Risco
Suínos
Transportes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-017-1008-5


  7 / 1464 MEDLINE  
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[PMID]:28331099
[Au] Autor:Li LF; Yu J; Zhang Y; Yang Q; Li Y; Zhang L; Wang J; Li S; Luo Y; Sun Y; Qiu HJ
[Ad] Endereço:State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
[Ti] Título:Interferon-Inducible Oligoadenylate Synthetase-Like Protein Acts as an Antiviral Effector against Classical Swine Fever Virus via the MDA5-Mediated Type I Interferon-Signaling Pathway.
[So] Source:J Virol;91(11), 2017 Jun 01.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), which poses a serious threat to the global pig industry. Interferons (IFNs) and IFN-stimulated genes (ISGs) play a key role in host antiviral defense. We have previously screened the porcine 2'-5'-oligoadenylate synthetase-like protein (pOASL) as a potential anti-CSFV ISG using a reporter CSFV. This study aimed to clarify the underlying antiviral mechanism of pOASL against CSFV. We confirmed that CSFV replication was significantly suppressed in lentivirus-delivered, pOASL-overexpressing PK-15 cells, whereas silencing the expression of endogenous pOASL by small interfering RNAs markedly enhanced CSFV growth. In addition, the transcriptional level of pOASL was upregulated both and upon CSFV infection. Interestingly, the anti-CSFV effects of pOASL are independent of the canonical RNase L pathway but depend on the activation of the type I IFN response. Glutathione -transferase pulldown and coimmunoprecipitation assays revealed that pOASL interacts with MDA5, a double-stranded RNA sensor, and further enhances MDA5-mediated type I IFN signaling. Moreover, we showed that pOASL exerts anti-CSFV effects in an MDA5-dependent manner. In conclusion, pOASL suppresses CSFV replication via the MDA5-mediated type I IFN-signaling pathway. The host innate immune response plays an important role in mounting the initial resistance to viral infection. Here, we identify the porcine 2'-5'-oligoadenylate synthetase-like protein (pOASL) as an interferon (IFN)-stimulated gene (ISG) against classical swine fever virus (CSFV). We demonstrate that the anti-CSFV effects of pOASL depend on the activation of type I IFN response. In addition, we show that pOASL, as an MDA5-interacting protein, is a coactivator of MDA5-mediated IFN induction to exert anti-CSFV actions. This work will be beneficial to the development of novel anti-CSFV strategies by targeting pOASL.
[Mh] Termos MeSH primário: 2´,5´-Oligoadenilato Sintetase/metabolismo
Vírus da Febre Suína Clássica/fisiologia
Interações Hospedeiro-Patógeno
Interferon Tipo I/metabolismo
Helicase IFIH1 Induzida por Interferon/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Peste Suína Clássica/imunologia
Peste Suína Clássica/virologia
Vírus da Febre Suína Clássica/crescimento & desenvolvimento
Endorribonucleases/genética
Endorribonucleases/metabolismo
Glutationa Transferase/metabolismo
Imunidade Inata
Imunoprecipitação
Interferon Tipo I/genética
Interferon Tipo I/imunologia
Helicase IFIH1 Induzida por Interferon/genética
Helicase IFIH1 Induzida por Interferon/imunologia
RNA Interferente Pequeno/genética
Transdução de Sinais
Suínos
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interferon Type I); 0 (RNA, Small Interfering); EC 2.5.1.18 (Glutathione Transferase); EC 2.7.7.84 (2',5'-Oligoadenylate Synthetase); EC 3.1.- (Endoribonucleases); EC 3.1.26.- (2-5A-dependent ribonuclease); EC 3.6.4.13 (Interferon-Induced Helicase, IFIH1)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170727
[Lr] Data última revisão:
170727
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE


  8 / 1464 MEDLINE  
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[PMID]:28284603
[Au] Autor:Luo Y; Ji S; Lei JL; Xiang GT; Liu Y; Gao Y; Meng XY; Zheng G; Zhang EY; Wang Y; Du ML; Li Y; Li S; He XJ; Sun Y; Qiu HJ
[Ad] Endereço:State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin 150069, PR China.
[Ti] Título:Efficacy evaluation of the C-strain-based vaccines against the subgenotype 2.1d classical swine fever virus emerging in China.
[So] Source:Vet Microbiol;201:154-161, 2017 Mar.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Classical swine fever (CSF) is a devastating infectious disease of pigs caused by classical swine fever virus (CSFV). The disease has been controlled following extensive vaccination with the lapinized attenuated vaccine C-strain for decades in China. However, frequent CSF outbreaks occurred recently in a large number of C-strain-vaccinated pig farms in China and a new subgenotype 2.1d of CSFV has been reported to be responsible for the outbreaks. Here we analyzed the molecular variations and antigenic differences among the C-strain-based commercial vaccines of different origins from different manufacturers in China, and reevaluated the vaccines against the emerging subgenotype 2.1d strain of CSFV. The results showed that the C-strain adapted to the continuous ST cell line (C ) contain a unique M290K variation on the E2 protein, compared to those of primary BT cells (C ) or rabbit origin (C ) and the traditional C-strain sequences available in the GenBank database. Serum neutralization test revealed the antigenic differences between C and C or C . Notably, the neutralizing titers of porcine anti-C-strain sera against the CSFV isolate of subgenotype 2.1d were significantly lower than those against C-strain or Shimen strain. The C-strain-vaccinated, subgenotype 2.1d HLJZZ2014 strain-challenged pigs did not show any clinical signs and all survived. However, these pigs displayed mild pathological and histological lesions, and the CSFV viral RNA was detected in the various tissue and blood samples. Taken together, the C-strain-based vaccines of different origins showed molecular variations and antigenic differences, and could provide clinical but not pathological and virological protection against the subgenotype 2.1d CSFV emerging in China. Further investigation is needed to comprehensively assess the efficacy of C-strain of different doses against the subgenotype 2.1d CSFV.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Peste Suína Clássica/prevenção & controle
Vírus da Febre Suína Clássica/imunologia
Vacinação/veterinária
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Variação Antigênica
China
Peste Suína Clássica/imunologia
Peste Suína Clássica/patologia
Peste Suína Clássica/virologia
Vírus da Febre Suína Clássica/genética
Vírus da Febre Suína Clássica/isolamento & purificação
Genótipo
Alinhamento de Sequência/veterinária
Suínos
Vacinas Atenuadas
Vacinas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Vaccines, Attenuated); 0 (Viral Vaccines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


  9 / 1464 MEDLINE  
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[PMID]:28284595
[Au] Autor:Coronado L; Liniger M; Muñoz-González S; Postel A; Pérez LJ; Pérez-Simó M; Perera CL; Frías-Lepoureau MT; Rosell R; Grundhoff A; Indenbirken D; Alawi M; Fischer N; Becher P; Ruggli N; Ganges L
[Ad] Endereço:Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba; IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.
[Ti] Título:Novel poly-uridine insertion in the 3'UTR and E2 amino acid substitutions in a low virulent classical swine fever virus.
[So] Source:Vet Microbiol;201:103-112, 2017 Mar.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In this study, we compared the virulence in weaner pigs of the Pinar del Rio isolate and the virulent Margarita strain. The latter caused the Cuban classical swine fever (CSF) outbreak of 1993. Our results showed that the Pinar del Rio virus isolated during an endemic phase is clearly of low virulence. We analysed the complete nucleotide sequence of the Pinar del Rio virus isolated after persistence in newborn piglets, as well as the genome sequence of the inoculum. The consensus genome sequence of the Pinar del Rio virus remained completely unchanged after 28days of persistent infection in swine. More importantly, a unique poly-uridine tract was discovered in the 3'UTR of the Pinar del Rio virus, which was not found in the Margarita virus or any other known CSFV sequences. Based on RNA secondary structure prediction, the poly-uridine tract results in a long single-stranded intervening sequence (SS) between the stem-loops I and II of the 3'UTR, without major changes in the stem- loop structures when compared to the Margarita virus. The possible implications of this novel insertion on persistence and attenuation remain to be investigated. In addition, comparison of the amino acid sequence of the viral proteins E , E1, E2 and p7 of the Margarita and Pinar del Rio viruses showed that all non-conservative amino acid substitutions acquired by the Pinar del Rio isolate clustered in E2, with two of them being located within the B/C domain. Immunisation and cross-neutralisation experiments in pigs and rabbits suggest differences between these two viruses, which may be attributable to the amino acid differences observed in E2. Altogether, these data provide fresh insights into viral molecular features which might be associated with the attenuation and adaptation of CSFV for persistence in the field.
[Mh] Termos MeSH primário: Regiões 3´ não Traduzidas/genética
Peste Suína Clássica/virologia
Vírus da Febre Suína Clássica
Proteínas do Envelope Viral/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Substituição de Aminoácidos
Animais
Sequência de Bases
Vírus da Febre Suína Clássica/genética
Vírus da Febre Suína Clássica/imunologia
Vírus da Febre Suína Clássica/isolamento & purificação
Vírus da Febre Suína Clássica/patogenicidade
Epitopos
Mutagênese Insercional
Coelhos
Alinhamento de Sequência/veterinária
Análise de Sequência de DNA/veterinária
Suínos
Uridina/genética
Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3' Untranslated Regions); 0 (Epitopes); 0 (Viral Envelope Proteins); 0 (glycoprotein E2, classical swine fever virus); WHI7HQ7H85 (Uridine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


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[PMID]:28279702
[Au] Autor:Khatoon E; Barman NN; Deka M; Rajbongshi G; Baruah K; Deka N; Bora DP; Kumar S
[Ad] Endereço:Department of Biotechnology, Gauhati University, Assam 781014, India; Department of Microbiology, College of Veterinary Science, Khanapara, Assam 781022, India.
[Ti] Título:Molecular characterization of classical swine fever virus isolates from India during 2012-14.
[So] Source:Acta Trop;170:184-189, 2017 Jun.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Classical swine fever is a highly contagious and economically important viral disease of pigs. Outbreaks of classical swine fever virus (CSFV) were recorded in different places in the Kamrup district of Assam in India between the years 2012 and 2014. The nucleotide sequences of the 10 CSFV isolates were analyzed based on the partial nucleotide sequences of the E2, 5'NTR and NS5B genes. Phylogenetic analysis indicated the dominance of subgroup 2.2 along with 2.1 strains in the northeast part of India. Variation in the nucleotide sequences of E2, 5'NTR and 3'NS5B genes of CSFV allows tracking changes in the virus population over time. The study will provide epidemiological information useful for assessing CSFV circulating genogroups in India.
[Mh] Termos MeSH primário: Peste Suína Clássica/epidemiologia
Vírus da Febre Suína Clássica
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Surtos de Doenças
Genótipo
Índia/epidemiologia
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE



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