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  1 / 2117 MEDLINE  
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[PMID]:28742120
[Au] Autor:Skappak C; Ilarraza R; Wu YQ; Drake MG; Adamko DJ
[Ad] Endereço:Division of Emergency Medicine, McMaster University, Hamilton, Ontario, Canada.
[Ti] Título:Virus-induced asthma attack: The importance of allergic inflammation in response to viral antigen in an animal model of asthma.
[So] Source:PLoS One;12(7):e0181425, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Asthma exacerbation can be a life-threatening condition, and is most often triggered by common respiratory viruses. Poor asthma control and worsening of respiratory function is associated with increased airway inflammation, including eosinophilia. Prevention of asthma exacerbation relies on treatment with corticosteroids, which preferentially inhibit allergic inflammation like eosinophils. Human studies demonstrate that inactivated virus can trigger eosinophil activation in vitro through antigen presentation and memory CD4+ lymphocytes. We hypothesized that animals with immunologic memory to a respiratory virus would also develop airway hyperresponsiveness in response to a UV-inactivated form of the virus if they have pre-existing allergic airway inflammation. Guinea pigs were ovalbumin-sensitized, infected with live parainfluenza virus (PIV), aerosol-challenged with ovalbumin, and then re-inoculated 60 days later with live or UV-inactivated PIV. Some animals were either treated with dexamethasone prior to the second viral exposure. Lymphocytes were isolated from parabronchial lymph nodes to confirm immunologic memory to the virus. Airway reactivity was measured and inflammation was assessed using bronchoalveolar lavage and lung histology. The induction of viral immunologic memory was confirmed in infected animals. Allergen sensitized and challenged animals developed airway hyperreactivity with eosinophilic airway inflammation when re-exposed to UV-inactivated PIV, while non-sensitized animals did not. Airway hyperreactivity in the sensitized animals was inhibited by pre-treatment with dexamethasone. We suggest that the response of allergic inflammation to virus antigen is a significant factor causing asthma exacerbation. We propose that this is one mechanism explaining how corticosteroids prevent virus-induced asthma attack.
[Mh] Termos MeSH primário: Asma/virologia
Vírus da Parainfluenza 1 Humana/imunologia
Hipersensibilidade Respiratória/virologia
Infecções por Respirovirus/complicações
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/uso terapêutico
Asma/tratamento farmacológico
Asma/imunologia
Dexametasona/uso terapêutico
Modelos Animais de Doenças
Feminino
Cobaias
Seres Humanos
Memória Imunológica/efeitos dos fármacos
Inflamação/tratamento farmacológico
Inflamação/imunologia
Inflamação/virologia
Linfócitos/imunologia
Linfócitos/virologia
Hipersensibilidade Respiratória/tratamento farmacológico
Hipersensibilidade Respiratória/imunologia
Infecções por Respirovirus/tratamento farmacológico
Infecções por Respirovirus/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 7S5I7G3JQL (Dexamethasone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181425


  2 / 2117 MEDLINE  
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[PMID]:28463659
[Au] Autor:Kosutic-Gulija T; Slovic A; Ljubin-Sternak S; Mlinaric-Galinovic G; Forcic D
[Ad] Endereço:1​Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, Zagreb, Croatia.
[Ti] Título:Genetic analysis of human parainfluenza virus type 3 obtained in Croatia, 2011-2015.
[So] Source:J Med Microbiol;66(4):502-510, 2017 Apr.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: This study investigated the HPIV3 circulating strains in Croatia and whether the other parts of HPIV3 genome (F gene and HN 582 nucleotides fragment) could be equally suitable for genetic and phylogenetic analysis. METHODOLOGY: Clinical materials were collected in period 2011-2015 from children suffering from respiratory illnesses. In positive HPIV3 samples viral genome was partially amplified and sequenced for HN and F genes. Obtained sequences were analysed by phylogenetic analysis and genetic characterization was performed. RESULTS: All samples from this study belonged to subcluster C and over a short period of time, genetic lineage C3a gained prevalence over the other C genetic lineages, from 39 % in 2011 to more than 90 % in 2013 and 2014. Phylogenetic classifications of HPIV3 based on the entire HN gene, HN 582 nt fragment and entire fusion (F) gene showed identical classification results for Croatian strains and the reference strains. Molecular analysis of the F and HN glycoproteins, showed their similar nucleotide diversity (Fcds P=0.0244 and HNcds P=0.0231) and similar Ka/Ks ratios (F Ka/Ks=0.0553 and HN Ka/Ks=0.0428). Potential N-glycosylation sites, cysteine residues and antigenic sites are generally strongly conserved in HPIV3 glycoproteins from both our and the reference samples. CONCLUSION: The HPIV3 subclaster C3 (genetic lineage C3a) became the most detected circulating HPIV3 strain in Croatia. The results indicated that the HN 582 nt and the entire F gene sequences were as good for phylogenetic analysis as the entire HN gene sequence.
[Mh] Termos MeSH primário: Genoma Viral/genética
Proteína HN/genética
Vírus da Parainfluenza 3 Humana/genética
Infecções por Respirovirus/epidemiologia
Proteínas Virais de Fusão/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Pré-Escolar
Croácia/epidemiologia
Seres Humanos
Lactente
Vírus da Parainfluenza 3 Humana/classificação
Vírus da Parainfluenza 3 Humana/isolamento & purificação
Filogenia
Infecções por Respirovirus/virologia
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (HN Protein); 0 (Viral Fusion Proteins)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000459


  3 / 2117 MEDLINE  
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[PMID]:28934360
[Au] Autor:Yan BR; Zhou L; Hu MM; Li M; Lin H; Yang Y; Wang YY; Shu HB
[Ad] Endereço:College of Life Sciences, Wuhan University, Wuhan, China.
[Ti] Título:PKACs attenuate innate antiviral response by phosphorylating VISA and priming it for MARCH5-mediated degradation.
[So] Source:PLoS Pathog;13(9):e1006648, 2017 Sep.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sensing of viral RNA by RIG-I-like receptors initiates innate antiviral response, which is mediated by the central adaptor VISA. How the RIG-I-VISA-mediated antiviral response is terminated at the late phase of infection is enigmatic. Here we identified the protein kinase A catalytic (PKAC) subunits α and ß as negative regulators of RNA virus-triggered signaling in a redundant manner. Viral infection up-regulated cellular cAMP levels and activated PKACs, which then phosphorylated VISA at T54. This phosphorylation abrogated virus-induced aggregation of VISA and primed it for K48-linked polyubiquitination and degradation by the E3 ligase MARCH5, leading to attenuation of virus-triggered induction of downstream antiviral genes. PKACs-deficiency or inactivation by the inhibitor H89 potentiated innate immunity to RNA viruses in cells and mice. Our findings reveal a critical mechanism of attenuating innate immune response to avoid host damage at the late phase of viral infection by the house-keeping PKA kinase.
[Mh] Termos MeSH primário: Proteínas Adaptadoras de Transdução de Sinal/imunologia
Subunidades Catalíticas da Proteína Quinase Dependente de AMP Cíclico/imunologia
Imunidade Inata/imunologia
Proteínas de Membrana/imunologia
Infecções por Respirovirus/imunologia
Ubiquitina-Proteína Ligases/imunologia
[Mh] Termos MeSH secundário: Animais
Células HEK293
Seres Humanos
Immunoblotting
Imunoprecipitação
Camundongos
Fosforilação
Vírus Sendai
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adaptor Proteins, Signal Transducing); 0 (Membrane Proteins); 0 (VISA protein, human); EC 2.3.2.27 (MARCH5 protein, human); EC 2.3.2.27 (Ubiquitin-Protein Ligases); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinase Catalytic Subunits)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006648


  4 / 2117 MEDLINE  
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[PMID]:28472480
[Au] Autor:Aguayo-Hiraldo PI; Arasaratnam RJ; Tzannou I; Kuvalekar M; Lulla P; Naik S; Martinez CA; Piedra PA; Vera JF; Leen AM
[Ad] Endereço:Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children's Hospital, and Houston Methodist Hospital.
[Ti] Título:Characterizing the Cellular Immune Response to Parainfluenza Virus 3.
[So] Source:J Infect Dis;216(2):153-161, 2017 Jul 15.
[Is] ISSN:1537-6613
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Parainfluenza virus type 3 (PIV3) infections are a major cause of morbidity and mortality in immunocompromised individuals, with no approved therapies. Our group has demonstrated the safety and efficacy of adoptively transferred virus-specific T cells for the prevention and treatment of a broad range of viral infections including BK virus, cytomegalovirus, adenovirus, human herpesvirus 6, and Epstein-Barr virus. However, this approach is restricted to well-characterized viruses with known immunogenic/protective T-cell target antigens, precluding extension to PIV3. We now characterize the cellular immune response to all 7 PIV3-encoded antigens in 17 healthy donors and define a hierarchy of immunogenicity based on the frequency of responding donors and the magnitude of specific cells. We show that reactive populations of both CD4+ and CD8+ T cells are capable of producing Th1-polarized effector cytokines and killing PIV3-expressing targets. Furthermore, we confirm the clinical relevance of these cells by demonstrating a direct correlation between the presence of PIV3-specific T cells and viral control in allogeneic hematopoietic stem cell transplant recipients. Taken together, our findings support the clinical use of PIV3-specific T cells produced with our Good Manufacturing Practice-compliant manufacturing process, in immunocompromised patients with uncontrolled infections.
[Mh] Termos MeSH primário: Antígenos Virais/imunologia
Imunidade Celular
Leucócitos Mononucleares/virologia
Vírus da Parainfluenza 3 Humana
Infecções por Respirovirus/imunologia
Linfócitos T/imunologia
[Mh] Termos MeSH secundário: Pré-Escolar
Citocinas/imunologia
Feminino
Seres Humanos
Imunoterapia
Lactente
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Viral); 0 (Cytokines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix203


  5 / 2117 MEDLINE  
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[PMID]:28276287
[Au] Autor:Cichero E; Tonelli M; Novelli F; Tasso B; Delogu I; Loddo R; Bruno O; Fossa P
[Ad] Endereço:a Department of Pharmacy , University of Genoa , Genoa , Italy.
[Ti] Título:Benzimidazole-based derivatives as privileged scaffold developed for the treatment of the RSV infection: a computational study exploring the potency and cytotoxicity profiles.
[So] Source:J Enzyme Inhib Med Chem;32(1):375-402, 2017 Dec.
[Is] ISSN:1475-6374
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Respiratory syncytial virus (RSV) has been identified as a main cause of hospitalisation in infants and children. To date, the current therapeutic arsenal is limited to ribavirin and palivizumab with variable efficacy. In this work, starting from a number of in-house series of previously described anti-RSV agents based on the benzimidazole scaffold, with the aim at gaining a better understanding of the related chemical features involved in potency and safety profiles, we applied a computational study including two focussed comparative molecular fields analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA). The results allowed us to derive useful suggestions for the design of derivatives and also to set up statistical models predicting the potency and selectivity index (SI = CC /EC ) of any new analogue prior to synthesis. Accordingly, here, we discuss preliminary results obtained through the applied exhaustive QSAR analyses, leading to design and synthesise more effective anti-RSV agents.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Benzimidazóis/farmacologia
Vírus Sinciciais Respiratórios/efeitos dos fármacos
Infecções por Respirovirus/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antivirais/síntese química
Antivirais/química
Benzimidazóis/síntese química
Benzimidazóis/química
Linhagem Celular
Cercopithecus aethiops
Relação Dose-Resposta a Droga
Seres Humanos
Testes de Sensibilidade Microbiana
Modelos Moleculares
Estrutura Molecular
Relação Quantitativa Estrutura-Atividade
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Benzimidazoles); E24GX49LD8 (benzimidazole)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170317
[Lr] Data última revisão:
170317
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170310
[St] Status:MEDLINE
[do] DOI:10.1080/14756366.2016.1256881


  6 / 2117 MEDLINE  
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[PMID]:28266286
[Au] Autor:Tsutsui R; Tsukagoshi H; Nagasawa K; Takahashi M; Matsushima Y; Ryo A; Kuroda M; Takami H; Kimura H
[Ad] Endereço:1​Aomori Prefecture Public Health and Environment Center, 1-1-1, Higashitsukurimichi, Aomori-shi, Aomori 030-8566, Japan 2​Department of Pathologic Analysis, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1, Hon-cho, Hirosaki-shi, Aomori 036-8564, J
[Ti] Título:Genetic analyses of the fusion protein genes in human parainfluenza virus types 1 and 3 among patients with acute respiratory infections in Eastern Japan from 2011 to 2015.
[So] Source:J Med Microbiol;66(2):160-168, 2017 Feb.
[Is] ISSN:1473-5644
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To genetically explore the fusion protein gene (F) in human parainfluenza virus type 1 (HPIV1) and type 3 (HPIV3) strains, we analysed them in patients with acute respiratory infections in Eastern Japan from 2011 to 2015. METHODOLOGY: We constructed phylogenetic trees based on the HPIV and HPIV3 F gene using the maximum likelihood method and conducted P-distance and selective pressure analyses. We also predicted the linear epitopes of the protein in the prototype strains. Furthermore, we mapped the amino acid substitutions of the proteins. RESULTS: Nineteen strains of HPIV1 and 53 strains of HPIV3 were detected among the clinical acute respiratory infection cases. The phylogenetic trees indicated that the HPIV1 and HPIV3 strains were classified into clusters II and III and cluster C, respectively. The P-distance values of the HPIV1 and HPIV3 F genes were <0.03. Two positive selection sites were inferred in the HPIV1 (aa 8 and aa 10), and one positive selection site was inferred in the HPIV3 (aa 108), but over 10 negative selection sites were inferred. Four epitopes were predicted for the HPIV1 prototype strains, while five epitopes were predicted for the HPIV3 prototype strain. A positive selection site (aa 108) or the HPIV3 F protein was involved in the predicted epitope. Additionally, we found that an amino acid substitution (R73K) in the LC76627 HPIV3 strain presumably may affect the resistance to neutralization by antibodies. CONCLUSION: The F gene of HPIV1 and HPIV3 was relatively well conserved in the eastern part of Japan during the investigation period.
[Mh] Termos MeSH primário: Vírus da Parainfluenza 1 Humana/genética
Vírus da Parainfluenza 3 Humana/genética
Infecções Respiratórias/epidemiologia
Infecções por Respirovirus/epidemiologia
Proteínas Virais de Fusão/genética
[Mh] Termos MeSH secundário: Doença Aguda
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Substituição de Aminoácidos
Criança
Pré-Escolar
Epitopos/genética
Epitopos/metabolismo
Feminino
Seres Humanos
Lactente
Japão/epidemiologia
Funções Verossimilhança
Masculino
Meia-Idade
Vírus da Parainfluenza 1 Humana/isolamento & purificação
Vírus da Parainfluenza 3 Humana/isolamento & purificação
Filogenia
RNA Viral/genética
RNA Viral/isolamento & purificação
Infecções Respiratórias/virologia
Infecções por Respirovirus/virologia
Análise de Sequência de RNA
Proteínas Virais de Fusão/metabolismo
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Epitopes); 0 (RNA, Viral); 0 (Viral Fusion Proteins); 109300-94-9 (F protein, parainfluenza virus 3)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170315
[Lr] Data última revisão:
170315
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170308
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000431


  7 / 2117 MEDLINE  
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[PMID]:28146439
[Au] Autor:Hsu BS; Jones-Sapienza SA
[Ad] Endereço:From the Departments of *Pediatrics, and †Surgery, Sanford School of Medicine, University of South Dakota, Sioux Falls, SD.
[Ti] Título:Air in All the Wrong Places.
[So] Source:Pediatr Emerg Care;33(2):107-108, 2017 Feb.
[Is] ISSN:1535-1815
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Extraluminal air can occur through a wide variety of mechanisms. Often, the free air resides in isolated regions including the thorax, the peritoneum, or the mediastinum. We present a pediatric case where there was extensive extraluminal air simultaneously within several regions, one of which has never been reported in the literature.
[Mh] Termos MeSH primário: Enfisema Mediastínico/diagnóstico por imagem
Pneumoperitônio/diagnóstico por imagem
Pneumotórax/diagnóstico por imagem
Enfisema Subcutâneo/diagnóstico por imagem
[Mh] Termos MeSH secundário: Feminino
Seres Humanos
Lactente
Vírus da Parainfluenza 3 Humana/isolamento & purificação
Insuficiência Respiratória/virologia
Infecções por Respirovirus/complicações
Infecções por Respirovirus/diagnóstico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170322
[Lr] Data última revisão:
170322
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170202
[St] Status:MEDLINE
[do] DOI:10.1097/PEC.0000000000000539


  8 / 2117 MEDLINE  
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[PMID]:28140644
[Au] Autor:Newcomer BW; Neill JD; Galik PK; Riddell KP; Zhang Y; Passler T; Velayudhan BT; Walz PH
[Ti] Título:Serologic survey for antibodies against three genotypes of bovine parainfluenza 3 virus in unvaccinated ungulates in Alabama.
[So] Source:Am J Vet Res;78(2):239-243, 2017 Feb.
[Is] ISSN:1943-5681
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE To determine titers of serum antibodies against 3 genotypes of bovine parainfluenza 3 virus (BPI3V) in unvaccinated ungulates in Alabama. ANIMALS 62 cattle, goats, and New World camelids from 5 distinct herds and 21 captured white-tailed deer. PROCEDURES Serum samples were obtained from all animals for determination of anti-BPI3V antibody titers, which were measured by virus neutralization assays that used indicator (reference) viruses from each of the 3 BPI3V genotypes (BPI3V-A, BPI3V-B, and BPI3V-C). The reference strains were recent clinical isolates from US cattle. Each sample was assayed in triplicate for each genotype. Animals with a mean antibody titer ≤ 2 for a particular genotype were considered seronegative for that genotype. RESULTS Animals seropositive for antibodies against BPI3V were identified in 2 of 3 groups of cattle and the group of New World camelids. The geometric mean antibody titer against BPI3V-B was significantly greater than that for BPI3V-A and BPI3V-C in all 3 groups. All goats, captive white-tailed deer, and cattle in the third cattle group were seronegative for all 3 genotypes of the virus. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that BPI3V-A may no longer be the predominant genotype circulating among ungulates in Alabama. This may be clinically relevant because BPI3V is frequently involved in the pathogenesis of bovine respiratory disease complex, current vaccines contain antigens against BPI3V-A only, and the extent of cross-protection among antibodies against the various BPI3V genotypes is unknown.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Vírus da Parainfluenza 3 Bovina/isolamento & purificação
Infecções por Respirovirus/veterinária
[Mh] Termos MeSH secundário: Alabama
Animais
Camelídeos Americanos
Bovinos
Cervos
Genótipo
Cabras
Vírus da Parainfluenza 3 Bovina/genética
Vírus da Parainfluenza 3 Bovina/imunologia
Infecções por Respirovirus/sangue
Infecções por Respirovirus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170201
[St] Status:MEDLINE
[do] DOI:10.2460/ajvr.78.2.239


  9 / 2117 MEDLINE  
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[PMID]:28129374
[Au] Autor:Muallem G; Wagage S; Sun Y; DeLong JH; Valenzuela A; Christian DA; Harms Pritchard G; Fang Q; Buza EL; Jain D; Elloso MM; López CB; Hunter CA
[Ad] Endereço:Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America.
[Ti] Título:IL-27 Limits Type 2 Immunopathology Following Parainfluenza Virus Infection.
[So] Source:PLoS Pathog;13(1):e1006173, 2017 Jan.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Respiratory paramyxoviruses are important causes of morbidity and mortality, particularly of infants and the elderly. In humans, a T helper (Th)2-biased immune response to these infections is associated with increased disease severity; however, little is known about the endogenous regulators of these responses that may be manipulated to ameliorate pathology. IL-27, a cytokine that regulates Th2 responses, is produced in the lungs during parainfluenza infection, but its role in disease pathogenesis is unknown. To determine whether IL-27 limits the development of pathogenic Th2 responses during paramyxovirus infection, IL-27-deficient or control mice were infected with the murine parainfluenza virus Sendai virus (SeV). Infected IL-27-deficient mice experienced increased weight loss, more severe lung lesions, and decreased survival compared to controls. IL-27 deficiency led to increased pulmonary eosinophils, alternatively activated macrophages (AAMs), and the emergence of Th2 responses. In control mice, IL-27 induced a population of IFN-γ+/IL-10+ CD4+ T cells that was replaced by IFN-γ+/IL-17+ and IFN-γ+/IL-13+ CD4+ T cells in IL-27-deficient mice. CD4+ T cell depletion in IL-27-deficient mice attenuated weight loss and decreased AAMs. Elimination of STAT6 signaling in IL-27-deficient mice reduced Th2 responses and decreased disease severity. These data indicate that endogenous IL-27 limits pathology during parainfluenza virus infection by regulating the quality of CD4+ T cell responses and therefore may have therapeutic potential in paramyxovirus infections.
[Mh] Termos MeSH primário: Linfócitos T CD4-Positivos/imunologia
Interleucinas/imunologia
Infecções por Respirovirus/imunologia
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Citometria de Fluxo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Reação em Cadeia da Polimerase em Tempo Real
Vírus Sendai/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Il27 protein, mouse); 0 (Interleukins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006173


  10 / 2117 MEDLINE  
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[PMID]:28120567
[Au] Autor:Hong KW; Choi SM; Lee DG; Cho SY; Lee HJ; Choi JK; Kim SH; Park SH; Choi JH; Yoo JH; Lee JW
[Ad] Endereço:Division of Infectious Diseases, Department of Internal Medicine, Hallym University College of Medicine, Chuncheon, Korea.
[Ti] Título:Lower Respiratory Tract Diseases Caused by Common Respiratory Viruses among Stem Cell Transplantation Recipients: A Single Center Experience in Korea.
[So] Source:Yonsei Med J;58(2):362-369, 2017 Mar.
[Is] ISSN:1976-2437
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To describe the incidence, clinical courses, and risk factors for mortality of lower respiratory tract diseases (LRDs) caused by common respiratory viruses (CRVs) in stem cell transplantation (SCT) recipients. MATERIALS AND METHODS: We retrospectively reviewed the medical records of 1038 patients who received SCT between January 2007 and August 2011 at a single center in Korea. RESULTS: Seventy-one CRV-LRDs were identified in 67 (6.5%) patients. The human parainfluenza virus (HPIV) was the most common causative pathogen of CRV-LRDs at 100 days [cumulative incidence estimate, 23.5%; 95% confidence interval (CI), 3.3-43.7] and 1 year (cumulative incidence estimate, 69.2%; 95% CI, 45.9-92.5) following SCT. The 30-day overall mortality rates due to influenza-LRDs, respiratory syncytial virus-LRDs, HPIV-LRDs, and human rhinovirus-LRDs were 35.7, 25.8, 31.6, and 42.8%, respectively. Co-pathogens in respiratory specimens were detected in 23 (33.8%) patients. The overall mortality at day 30 after CRV-LRD diagnosis was 32.8% (22/67). High-dose steroid usage (p=0.025), a severe state of immunodeficiency (p=0.033), and lymphopenia (p=0.006) were significantly associated with death within 30 days following CRV-LRD diagnosis in a univariate analysis. Multivariate logistic regression analysis revealed that high-dose steroid usage [odds ratio (OR), 4.05; 95% CI, 1.12-14.61; p=0.033] and lymphopenia (OR, 6.57; 95% CI, 1.80-24.03; p=0.004) were independent risk factors for mortality within 30 days of CRV-LRDs. CONCLUSION: CRV-LRDs among SCT recipients showed substantially high morbidity and mortality rates. Therefore, the implement of an active diagnostic approaches for CRV infections is required for SCT recipients with respiratory symptoms, especially those receiving high-dose steroids or with lymphopenia.
[Mh] Termos MeSH primário: Influenza Humana/virologia
Infecções por Vírus Respiratório Sincicial/virologia
Vírus Sinciciais Respiratórios
Infecções Respiratórias/virologia
Infecções por Respirovirus/virologia
Transplante de Células-Tronco
[Mh] Termos MeSH secundário: Adulto
Feminino
Transplante de Células-Tronco Hematopoéticas/efeitos adversos
Seres Humanos
Síndromes de Imunodeficiência/mortalidade
Incidência
Influenza Humana/epidemiologia
Influenza Humana/mortalidade
Linfopenia/mortalidade
Masculino
Meia-Idade
República da Coreia
Infecções por Vírus Respiratório Sincicial/epidemiologia
Infecções por Vírus Respiratório Sincicial/mortalidade
Infecções Respiratórias/epidemiologia
Infecções Respiratórias/mortalidade
Infecções por Respirovirus/epidemiologia
Infecções por Respirovirus/mortalidade
Estudos Retrospectivos
Fatores de Risco
Transplante de Células-Tronco/mortalidade
Esteroides/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Steroids)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE
[do] DOI:10.3349/ymj.2017.58.2.362



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