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[PMID]:27984784
[Au] Autor:Zhu X; Fang L; Wang D; Yang Y; Chen J; Ye X; Foda MF; Xiao S
[Ad] Endereço:State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, China.
[Ti] Título:Porcine deltacoronavirus nsp5 inhibits interferon-ß production through the cleavage of NEMO.
[So] Source:Virology;502:33-38, 2017 Feb.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Porcine deltacoronavirus (PDCoV) causes acute enteric disease and mortality in seronegative neonatal piglets. Previously we have demonstrated that PDCoV infection suppresses the production of interferon-beta (IFN-ß), while the detailed mechanisms are poorly understood. Here, we demonstrate that nonstructural protein 5 (nsp5) of PDCoV, the 3C-like protease, significantly inhibits Sendai virus (SEV)-induced IFN-ß production by targeting the NF-κB essential modulator (NEMO), confirmed by the diminished function of NEMO cleaved by PDCoV. The PDCoV nsp5 cleavage site in the NEMO protein was identified as glutamine 231, and was identical to the porcine epidemic diarrhea virus nsp5 cleavage site, revealing the likelihood of a common target in NEMO for coronaviruses. Furthermore, this cleavage impaired the ability of NEMO to activate the IFN response and downstream signaling. Taken together, our findings reveal PDCoV nsp5 to be a newly identified IFN antagonist and enhance the understanding of immune evasion by deltacoronaviruses.
[Mh] Termos MeSH primário: Infecções por Coronaviridae/veterinária
Coronaviridae/enzimologia
Cisteína Endopeptidases/metabolismo
Quinase I-kappa B/metabolismo
Interferon beta/metabolismo
Doenças dos Suínos/enzimologia
Proteínas não Estruturais Virais/metabolismo
[Mh] Termos MeSH secundário: Animais
Coronaviridae/genética
Infecções por Coronaviridae/enzimologia
Infecções por Coronaviridae/metabolismo
Infecções por Coronaviridae/virologia
Cisteína Endopeptidases/genética
Interações Hospedeiro-Patógeno
Quinase I-kappa B/genética
Interferon beta/genética
Processamento de Proteína Pós-Traducional
Suínos
Doenças dos Suínos/genética
Doenças dos Suínos/metabolismo
Doenças dos Suínos/virologia
Proteínas não Estruturais Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Nonstructural Proteins); 77238-31-4 (Interferon-beta); EC 2.7.11.10 (I-kappa B Kinase); EC 3.4.22.- (3C-like proteinase, Coronavirus); EC 3.4.22.- (Cysteine Endopeptidases)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


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[PMID]:27718337
[Au] Autor:Saeng-Chuto K; Lorsirigool A; Temeeyasen G; Vui DT; Stott CJ; Madapong A; Tripipat T; Wegner M; Intrakamhaeng M; Chongcharoen W; Tantituvanont A; Kaewprommal P; Piriyapongsa J; Nilubol D
[Ad] Endereço:Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
[Ti] Título:Different Lineage of Porcine Deltacoronavirus in Thailand, Vietnam and Lao PDR in 2015.
[So] Source:Transbound Emerg Dis;64(1):3-10, 2017 Feb.
[Is] ISSN:1865-1682
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Porcine deltacoronavirus (PDCoV) was detected by RT-PCR in 12 of 97 (12.4%) intestinal samples collected during 2015 from piglets with diarrhoea in Thailand, Vietnam and Lao PDR. Spike, membrane and nucleocapsid genes were characterized, and phylogenetic analyses demonstrated that PDCoV isolates from Thai and Lao PDR form a novel cluster, separated from US and China isolates, but relatively were more closely related to China PDCoV than US isolates. Vietnam PDCoVs, however, were grouped together with US PDCoV. The analyses of amino acid changes suggested that they were from different lineage.
[Mh] Termos MeSH primário: Coronaviridae/genética
DNA Viral/genética
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Infecções por Coronaviridae/genética
Intestinos/virologia
Laos
Filogenia
Reação em Cadeia da Polimerase
Suínos
Tailândia
Vietnã
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170209
[Lr] Data última revisão:
170209
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161009
[St] Status:MEDLINE
[do] DOI:10.1111/tbed.12585


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[PMID]:27931933
[Au] Autor:Gerber PF; Lelli D; Zhang J; Strandbygaard B; Moreno A; Lavazza A; Perulli S; Bøtner A; Comtet L; Roche M; Pourquier P; Wang C; Opriessnig T
[Ad] Endereço:The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom.
[Ti] Título:Diagnostic evaluation of assays for detection of antibodies against porcine epidemic diarrhea virus (PEDV) in pigs exposed to different PEDV strains.
[So] Source:Prev Vet Med;135:87-94, 2016 Dec 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Porcine epidemic diarrhea virus (PEDV) has caused economic losses in the Americas, Asia and Europe in recent years. Reliable serological assays are essential for epidemiological studies and vaccine evaluation. The objective of this study was to compare the ability of five enzyme-linked immunosorbent assays (ELISAs) to detect antibodies against different PEDV strains in pig serum. A total of 732 serum samples from North American or European pigs were tested. Samples included experimental samples from pigs infected with classical (G1a PEDV) or variant genogroup 1 PEDV (G1b PEDV), pandemic genogroup 2 PEDV (G2b PEDV) or non-infected controls. Field samples from herds with confirmed or unknown PEDV exposure were also used. Three indirect ELISAs based on G2b antigens (ELISAs 1, 2 and 3), a competitive ELISA based on the G2b antigen (ELISA 4) and a competitive ELISA based on the G1a antigen (ELISA 5) were compared. Overall, the tests had a moderate agreement (κ=0.61). G1a PEDV infected pigs were earliest detected by ELISA 3, G1b PEDV infected pigs were earliest detected by ELISAs 4 and 5 and the performance of all tests was similar for the G2b PEDV group. ELISA 1 showed the overall lowest detection on experimentally and field derived samples. Diagnostic sensitivity and specificity with a 95% probability interval were estimated to be 68.2% (62.1-74.4%) and 97.5% (95.2-99.0%) for ELISA 1, 73.7% (71.5-79.6%) and 98.4% (96.6-99.5%) for ELISA 2, 86.2% (81.1-90.6%) and 91.6% (87.7-94.8%) for ELISA 3, 78.3% (72.8-83.5%) and 99.7% (98.2-100%) for ELISA 4, and 93.5% (90.3-96.0%) and 91.2% (83.8-97.9%) for ELISA 5. Differences in detection among assays seem to be more related to intrinsic factors of an assay than to the PEDV antigen used.
[Mh] Termos MeSH primário: Anticorpos Antivirais/sangue
Infecções por Coronaviridae/veterinária
Técnicas e Procedimentos Diagnósticos/veterinária
Ensaio de Imunoadsorção Enzimática/veterinária
Vírus da Diarreia Epidêmica Suína/isolamento & purificação
Doenças dos Suínos/diagnóstico
[Mh] Termos MeSH secundário: Animais
Infecções por Coronaviridae/diagnóstico
Infecções por Coronaviridae/virologia
Dinamarca
Itália
Sensibilidade e Especificidade
Suínos
Doenças dos Suínos/virologia
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE


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[PMID]:27599927
[Au] Autor:Moutelíková R; Dufková L; Kamler J; Drimaj J; Plhal R; Prodelalová J
[Ad] Endereço:Department of Virology, Veterinary Research Institute, Hudcova 70, 62100 Brno, Czech Republic. Electronic address: moutelikova@vri.cz.
[Ti] Título:Epidemiological survey of enteric viruses in wild boars in the Czech Republic: First evidence of close relationship between wild boar and human rotavirus A strains.
[So] Source:Vet Microbiol;193:28-35, 2016 Sep 25.
[Is] ISSN:1873-2542
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Population of wild boar is increasing in the whole Europe, the animals migrate close to human habitats which greatly increases the possibility of natural transmission between domestic animals or humans and wild boars. The aim of the study was to estimate in population of free-living wild boar in the Czech Republic the prevalence of enteric viral pathogens, namely rotavirus groups A and C (RVA and RVC), porcine reproductive and respiratory syndrome virus (PRRSV), and members of family Coronaviridae (transmissible gastroenteritis virus - TGEV, porcine epidemic diarrhea virus - PEDV, porcine respiratory coronavirus - PRCV, and porcine hemagglutination encephalomyelitis virus - PHEV) and Picornaviridae,(teschovirus A - PTV, sapelovirus A - PSV, and enterovirus G - EV-G). In our study, stool samples from 203 wild boars culled during hunting season 2014-2015 (from October to January) were examined by RT-PCR. RVA was detected in 2.5% of tested samples. Nucleotide analysis of VP7, VP4, and VP6 genes revealed that four RVA strains belong to G4P[25]I1, G4P[6]I5, G11P[13]I5, and G5P[13]I5 genotypes and phylogenetic analysis suggested close relation to porcine and human RVAs. The prevalence of RVC in wild boar population reached 12.8%, PTV was detected in 20.2%, PSV in 8.9%, and EV-G in 2.5% of samples. During our study no PRRSV or coronaviruses were detected. Our study provides the first evidence of RVC prevalence in wild boars and indicates that wild boars might contribute to the genetic variability of RVA and also serve as an important reservoir of other enteric viruses.
[Mh] Termos MeSH primário: Infecções por Coronaviridae/veterinária
Infecções por Picornaviridae/veterinária
Infecções por Rotavirus/veterinária
Rotavirus/isolamento & purificação
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
Antígenos Virais/genética
Proteínas do Capsídeo/genética
Coronaviridae/genética
Coronaviridae/isolamento & purificação
Infecções por Coronaviridae/epidemiologia
Infecções por Coronaviridae/virologia
República Tcheca/epidemiologia
Reservatórios de Doenças
Fezes/virologia
Feminino
Genótipo
Seres Humanos
Masculino
Filogenia
Picornaviridae/genética
Picornaviridae/isolamento & purificação
Infecções por Picornaviridae/epidemiologia
Infecções por Picornaviridae/virologia
Rotavirus/genética
Infecções por Rotavirus/epidemiologia
Infecções por Rotavirus/virologia
Sus scrofa
Suínos
Doenças dos Suínos/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antigens, Viral); 0 (Capsid Proteins); 0 (VP4 protein, Rotavirus); 0 (VP6 protein, Rotavirus); 0 (VP7 protein, Rotavirus)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160908
[St] Status:MEDLINE


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[PMID]:27496131
[Au] Autor:Zhai SL; Wei WK; Li XP; Wen XH; Zhou X; Zhang H; Lv DH; Li F; Wang D
[Ad] Endereço:Animal Disease Diagnostic Center, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Animal Disease Prevention, Guangdong Open Laboratory of Veterinary Public Health, Guangzhou, 510640, China.
[Ti] Título:Occurrence and sequence analysis of porcine deltacoronaviruses in southern China.
[So] Source:Virol J;13:136, 2016 Aug 05.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Following the initial isolation of porcine deltacoronavirus (PDCoV) from pigs with diarrheal disease in the United States in 2014, the virus has been detected on swine farms in some provinces of China. To date, little is known about the molecular epidemiology of PDCoV in southern China where major swine production is operated. RESULTS: To investigate the prevalence of PDCoV in this region and compare its activity to other enteric disease of swine caused by porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis coronavirus (TGEV), and porcine rotavirus group C (Rota C), 390 fecal samples were collected from swine of various ages from 15 swine farms with reported diarrhea. Fecal samples were tested by reverse transcription-PCR (RT-PCR) that targeted PDCoV, PEDV, TGEV, and Rota C, respectively. PDCoV was detected exclusively from nursing piglets with an overall prevalence of approximate 1.28 % (5/390), not in suckling and fattening piglets. Interestingly, all of PDCoV-positive samples were from 2015 rather than 2012-2014. Despite a low detection rate, PDCoV emerged in each province/region of southern China. In addition, compared to TGEV (1.54 %, 5/390) or Rota C (1.28 %, 6/390), there were highly detection rates of PEDV (22.6 %, 88/390) in those samples. Notably, all five PDCoV-positive piglets were co-infected by PEDV. Furthermore, phylogenetic analysis of spike (S) and nucleocapsid (N) gene sequences of PDCoVs revealed that currently circulating PDCoVs in southern China were more closely related to other Chinese strains of PDCoVs than to those reported in United States, South Korea and Thailand. CONCLUSIONS: This study demonstrated that PDCoV was present in southern China despite the low prevalence, and supported an evolutionary theory of geographical clustering of PDCoVs.
[Mh] Termos MeSH primário: Infecções por Coronaviridae/veterinária
Coronaviridae/isolamento & purificação
Fezes/virologia
Doenças dos Suínos/virologia
[Mh] Termos MeSH secundário: Animais
China/epidemiologia
Coronaviridae/classificação
Coronaviridae/genética
Infecções por Coronaviridae/epidemiologia
Infecções por Coronaviridae/virologia
Filogenia
Análise de Sequência de DNA
Suínos
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160807
[St] Status:MEDLINE
[do] DOI:10.1186/s12985-016-0591-6


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[PMID]:27479465
[Au] Autor:Rüdiger AT; Mayrhofer P; Ma-Lauer Y; Pohlentz G; Müthing J; von Brunn A; Schwegmann-Weßels C
[Ad] Endereço:Institute of Virology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany.
[Ti] Título:Tubulins interact with porcine and human S proteins of the genus Alphacoronavirus and support successful assembly and release of infectious viral particles.
[So] Source:Virology;497:185-197, 2016 Oct.
[Is] ISSN:1096-0341
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Coronavirus spike proteins mediate host-cell-attachment and virus entry. Virus replication takes place within the host cell cytosol, whereas assembly and budding occur at the endoplasmic reticulum-Golgi intermediate compartment. In this study we demonstrated that the last 39 amino acid stretches of Alphacoronavirus spike cytoplasmic domains of the human coronavirus 229E, NL63, and the porcine transmissible gastroenteritis virus TGEV interact with tubulin alpha and beta chains. In addition, a partial co-localization of TGEV spike proteins with authentic host cell ß-tubulin was observed. Furthermore, drug-induced microtubule depolymerization led to changes in spike protein distribution, a reduction in the release of infectious virus particles and less amount of spike protein incorporated into virions. These data demonstrate that interaction of Alphacoronavirus spike proteins with tubulin supports S protein transport and incorporation into virus particles.
[Mh] Termos MeSH primário: Infecções por Coronaviridae/metabolismo
Infecções por Coronaviridae/virologia
Coronaviridae/fisiologia
Glicoproteína da Espícula de Coronavírus/metabolismo
Tubulina (Proteína)/metabolismo
Montagem de Vírus
Replicação Viral
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Coronaviridae/efeitos dos fármacos
Gastroenterite Suína Transmissível/metabolismo
Gastroenterite Suína Transmissível/virologia
Seres Humanos
Espaço Intracelular/metabolismo
Espaço Intracelular/virologia
Nocodazol/farmacologia
Ligação Proteica
Domínios e Motivos de Interação entre Proteínas
Transporte Proteico
Glicoproteína da Espícula de Coronavírus/química
Suínos
Montagem de Vírus/efeitos dos fármacos
Liberação de Vírus
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Spike Glycoprotein, Coronavirus); 0 (Tubulin); SH1WY3R615 (Nocodazole)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160802
[St] Status:MEDLINE


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[PMID]:27384656
[Au] Autor:Guo L; Luo X; Li R; Xu Y; Zhang J; Ge J; Bu Z; Feng L; Wang Y
[Ad] Endereço:State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
[Ti] Título:Porcine Epidemic Diarrhea Virus Infection Inhibits Interferon Signaling by Targeted Degradation of STAT1.
[So] Source:J Virol;90(18):8281-92, 2016 Sep 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: Porcine epidemic diarrhea virus (PEDV) is a worldwide-distributed alphacoronavirus, but the pathogenesis of PEDV infection is not fully characterized. During virus infection, type I interferon (IFN) is a key mediator of innate antiviral responses. Most coronaviruses develop some strategy for at least partially circumventing the IFN response by limiting the production of IFN and by delaying the activation of the IFN response. However, the molecular mechanisms by which PEDV antagonizes the antiviral effects of interferon have not been fully characterized. Especially, how PEDV impacts IFN signaling components has yet to be elucidated. In this study, we observed that PEDV was relatively resistant to treatment with type I IFN. Western blot analysis showed that STAT1 expression was markedly reduced in PEDV-infected cells and that this reduction was not due to inhibition of STAT1 transcription. STAT1 downregulation was blocked by a proteasome inhibitor but not by an autophagy inhibitor, strongly implicating the ubiquitin-proteasome targeting degradation system. Since PEDV infection-induced STAT1 degradation was evident in cells pretreated with the general tyrosine kinase inhibitor, we conclude that STAT1 degradation is independent of the IFN signaling pathway. Furthermore, we report that PEDV-induced STAT1 degradation inhibits IFN-α signal transduction pathways. Pharmacological inhibition of STAT1 degradation rescued the ability of the host to suppress virus replication. Collectively, these data show that PEDV is capable of subverting the type I interferon response by inducing STAT1 degradation. IMPORTANCE: In this study, we show that PEDV is resistant to the antiviral effect of IFN. The molecular mechanism is the degradation of STAT1 by PEDV infection in a proteasome-dependent manner. This PEDV infection-induced STAT1 degradation contributes to PEDV replication. Our findings reveal a new mechanism evolved by PEDV to circumvent the host antiviral response.
[Mh] Termos MeSH primário: Antivirais/antagonistas & inibidores
Interações Hospedeiro-Patógeno
Evasão da Resposta Imune
Interferon-alfa/antagonistas & inibidores
Vírus da Diarreia Epidêmica Suína/patogenicidade
Fator de Transcrição STAT1/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Western Blotting
Linhagem Celular
Cercopithecus aethiops
Infecções por Coronaviridae
Regulação para Baixo
Proteólise
Fator de Transcrição STAT1/metabolismo
Transdução de Sinais
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Interferon-alpha); 0 (STAT1 Transcription Factor)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170508
[Lr] Data última revisão:
170508
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160708
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.01091-16


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[PMID]:27309279
[Au] Autor:European Union COST Action FA1207
[Ti] Título:Recommendations for a Standardized Avian Coronavirus (AvCoV) Nomenclature: Outcome from Discussions Within the Framework of the European Union COST Action FA1207: "Towards Control of Avian Coronaviruses: Strategies for Vaccination, Diagnosis and Surveillance".
[So] Source:Avian Dis;60(2):411-2, 2016 Jun.
[Is] ISSN:1938-4351
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Viruses within the Coronaviridae family show variations within their genome sequences, especially within the major structural protein, the Spike (S) glycoprotein gene. Therefore, many different antigenic forms, serotypes, or variant strains of avian coronaviruses (AvCoV) exist worldwide. Only a few of them, the so called protectotypes, cross protect against different serotypes. New serotypes arise by recombination or spontaneous mutations. From time to time, antigenic virus variants appear which differ significantly from known serotypes. The result of this variability is an inconsistent nomenclature and classification of virus strains. Furthermore, there are currently no standard classification methods defined. Within the framework of the COST Action FA1207 "Towards control of avian coronaviruses: strategies for diagnosis, surveillance, and vaccination" (working groups "Molecular virology" and "Epidemiology"), we aimed at defining and developing a unified and internationally standardized nomenclature and classification of AvCoVs. We recommend the use of "CoV Genus/AvCov/host/country/specimen id/year" to refer to AvCoV strains.
[Mh] Termos MeSH primário: Doenças das Aves/classificação
Infecções por Coronaviridae/veterinária
Coronaviridae/classificação
[Mh] Termos MeSH secundário: Animais
Doenças das Aves/virologia
Infecções por Coronaviridae/classificação
Infecções por Coronaviridae/virologia
Terminologia como Assunto
[Pt] Tipo de publicação:LETTER
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160617
[St] Status:MEDLINE
[do] DOI:10.1637/0005-2086-60.2.411


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[PMID]:27277214
[Au] Autor:Okda F; Lawson S; Liu X; Singrey A; Clement T; Hain K; Nelson J; Christopher-Hennings J; Nelson EA
[Ad] Endereço:Veterinary & Biomedical Sciences Department, South Dakota State University, Brookings, SD, USA.
[Ti] Título:Development of monoclonal antibodies and serological assays including indirect ELISA and fluorescent microsphere immunoassays for diagnosis of porcine deltacoronavirus.
[So] Source:BMC Vet Res;12:95, 2016 Jun 08.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: A novel porcine deltacoronavirus (PDCoV), also known as porcine coronavirus HKU15, was reported in China in 2012 and identified in the U.S. in early 2014. Since then, PDCoV has been identified in a number of U.S. states and linked with clinical disease including acute diarrhea and vomiting in the absence of other identifiable pathogens. Since PDCoV was just recently linked with clinical disease, few specific antibody-based reagents were available to assist in diagnosis of PDCoV and limited serological capabilities were available to detect an antibody response to this virus. Therefore, the overall objective of this project was to develop and validate selected diagnostic reagents and assays for PDCoV antigen and antibody detection. RESULTS: The nucleoprotein of PDCoV was expressed as a recombinant protein and purified for use as an antigen to immunize mice for polyclonal, hyperimmune sera and monoclonal antibody (mAb) production. The resulting mAbs were evaluated for use in fluorescent antibody staining methods to detect PDCoV infected cells following virus isolation attempts and for immunohistochemistry staining of intestinal tissues of infected pigs. The same antigen was used to develop serological tests to detect the antibody response to PDCoV in pigs following infection. Serum samples from swine herds with recent documentation of PDCoV infection and samples from expected naïve herds were used for initial assay optimization. The tests were optimized in a checkerboard fashion to reduce signal to noise ratios using samples of known status. Statistical analysis was performed to establish assay cutoff values and assess diagnostic sensitivities and specificities. At least 629 known negative serum samples and 311 known positive samples were evaluated for each assay. The enzyme linked immunosorbent assay (ELISA) showed diagnostic sensitivity (DSe) of 96.1% and diagnostic specificity (DSp) of 96.2%. The fluorescent microsphere immunoassay (FMIA) showed a DSe of 95.8% and DSp of 98.1%. Both ELISA and FMIA detected seroconversion of challenged pigs between 8-14 days post-infection (DPI). An indirect fluorescent antibody (IFA) test was also developed using cell culture adapted PDCoV for comparative purposes. CONCLUSION: These new, specific reagents and serological assays will allow for improved diagnosis of PDCoV. Since many aspects of PDCoV infection and transmission are still not fully understood, the reagents and assays developed in this project should provide valuable tools to help understand this disease and to aid in the control and surveillance of porcine deltacoronavirus outbreaks.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/imunologia
Anticorpos Antivirais/imunologia
Infecções por Coronaviridae/veterinária
Coronaviridae/imunologia
Ensaio de Imunoadsorção Enzimática/veterinária
Doenças dos Suínos/diagnóstico
[Mh] Termos MeSH secundário: Animais
Antígenos Virais/imunologia
Células Cultivadas
Infecções por Coronaviridae/diagnóstico
Infecções por Coronaviridae/imunologia
Reações Cruzadas
Ensaio de Imunoadsorção Enzimática/métodos
Técnica Indireta de Fluorescência para Anticorpo/métodos
Microesferas
Nucleoproteínas/imunologia
Dobramento de Proteína
Testes Sorológicos/métodos
Testes Sorológicos/veterinária
Suínos
Doenças dos Suínos/imunologia
Doenças dos Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Viral); 0 (Antigens, Viral); 0 (Nucleoproteins)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160610
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-016-0716-6


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[PMID]:26976607
[Au] Autor:Menachery VD; Yount BL; Sims AC; Debbink K; Agnihothram SS; Gralinski LE; Graham RL; Scobey T; Plante JA; Royal SR; Swanstrom J; Sheahan TP; Pickles RJ; Corti D; Randell SH; Lanzavecchia A; Marasco WA; Baric RS
[Ad] Endereço:Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599;
[Ti] Título:SARS-like WIV1-CoV poised for human emergence.
[So] Source:Proc Natl Acad Sci U S A;113(11):3048-53, 2016 Mar 15.
[Is] ISSN:1091-6490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Outbreaks from zoonotic sources represent a threat to both human disease as well as the global economy. Despite a wealth of metagenomics studies, methods to leverage these datasets to identify future threats are underdeveloped. In this study, we describe an approach that combines existing metagenomics data with reverse genetics to engineer reagents to evaluate emergence and pathogenic potential of circulating zoonotic viruses. Focusing on the severe acute respiratory syndrome (SARS)-like viruses, the results indicate that the WIV1-coronavirus (CoV) cluster has the ability to directly infect and may undergo limited transmission in human populations. However, in vivo attenuation suggests additional adaptation is required for epidemic disease. Importantly, available SARS monoclonal antibodies offered success in limiting viral infection absent from available vaccine approaches. Together, the data highlight the utility of a platform to identify and prioritize prepandemic strains harbored in animal reservoirs and document the threat posed by WIV1-CoV for emergence in human populations.
[Mh] Termos MeSH primário: Quirópteros/virologia
Doenças Transmissíveis Emergentes/virologia
Infecções por Coronaviridae/virologia
Coronaviridae/patogenicidade
[Mh] Termos MeSH secundário: Animais
Anticorpos Monoclonais/imunologia
Anticorpos Neutralizantes/imunologia
Anticorpos Antivirais/imunologia
Células Cultivadas
Cercopithecus aethiops
Coronaviridae/genética
Coronaviridae/imunologia
Coronaviridae/isolamento & purificação
Coronaviridae/fisiologia
Infecções por Coronaviridae/prevenção & controle
Infecções por Coronaviridae/transmissão
Infecções por Coronaviridae/veterinária
Reações Cruzadas
Encefalite Viral/virologia
Células Epiteliais/virologia
Especificidade de Hospedeiro
Seres Humanos
Pulmão/citologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Transgênicos
Modelos Moleculares
Peptidil Dipeptidase A/genética
Peptidil Dipeptidase A/fisiologia
Mutação Puntual
Conformação Proteica
Receptores Virais/genética
Receptores Virais/fisiologia
Proteínas Recombinantes de Fusão/metabolismo
Vírus da SARS/imunologia
Especificidade da Espécie
Glicoproteína da Espícula de Coronavírus/genética
Glicoproteína da Espícula de Coronavírus/fisiologia
Células Vero
Replicação Viral
Zoonoses
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Receptors, Virus); 0 (Recombinant Fusion Proteins); 0 (Spike Glycoprotein, Coronavirus); EC 3.4.15.1 (Peptidyl-Dipeptidase A); EC 3.4.17.- (angiotensin converting enzyme 2)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160316
[St] Status:MEDLINE
[do] DOI:10.1073/pnas.1517719113



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