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[PMID]:26616156
[Au] Autor:Tsuchiaka S; Masuda T; Sugimura S; Kobayashi S; Komatsu N; Nagai M; Omatsu T; Furuya T; Oba M; Katayama Y; Kanda S; Yokoyama T; Mizutani T
[Ad] Endereço:The United Graduate School of Veterinary Sciences, Gifu University, Yanagito, Gifu 501-1193, Japan.
[Ti] Título:Development of a novel detection system for microbes from bovine diarrhea by real-time PCR.
[So] Source:J Vet Med Sci;78(3):383-9, 2016 Mar.
[Is] ISSN:1347-7439
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Diarrhea in cattle is one of the most economically costly disorders, decreasing milk production and weight gain. In the present study, we established a novel simultaneous detection system using TaqMan real-time PCR designed as a system for detection of microbes from bovine diarrhea using real-time PCR (referred to as Dembo-PCR). Dembo-PCR simultaneously detects a total of 19 diarrhea-causing pathogens, including viruses, bacteria and protozoa. Specific primer-probe sets were newly designed for 7 pathogens and were synthesized on the basis of previous reports for 12 pathogens. Assays were optimized to react under the same reaction conditions. The PCR efficiency and correlation coefficient (R(2)) of standard curves for each assay were more than 80% and 0.9766, respectively. Furthermore, the sensitivity of Dembo-PCR in fecal sample analysis was measured with feces spiked with target pathogens or synthesized DNA that included specific nucleotide target regions. The resulting limits of detection (LOD) for virus-spiked samples, bacteria and DNA fragments were 0.16-1.6 TCID50 (PFU/reaction), 1.3-13 CFU/reaction and 10-100 copies/reaction, respectively. All reactions showed high sensitivity in pathogen detection. A total of 8 fecal samples, collected from 6 diarrheic cattle, 1 diarrheic calf and 1 healthy cow, were tested using Dembo-PCR to validate the assay's clinical performance. The results revealed that bovine coronavirus had infected all diarrheic adult cattle and that bovine torovirus had infected the diarrheic calf. These results suggest that Dembo-PCR may be a powerful tool for diagnosing infectious agents in cattle diarrhea.
[Mh] Termos MeSH primário: Doenças dos Bovinos/microbiologia
Diarreia/microbiologia
Reação em Cadeia da Polimerase em Tempo Real/veterinária
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/diagnóstico
Infecções por Coronavirus/diagnóstico
Infecções por Coronavirus/veterinária
Coronavirus Bovino
Diarreia/diagnóstico
Feminino
Torovirus
Infecções por Torovirus/diagnóstico
Infecções por Torovirus/veterinária
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151201
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.15-0552


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[PMID]:26611229
[Au] Autor:Liu X; Zhou Y; Yang F; Liu P; Cai Y; Huang J; Zhu L; Xu Z
[Ad] Endereço:Key Laboratory of Animal Biotechnology Center, College of Veterinary Medicine of Sichuan Agricultural University, Huimin Road 211, Wenjiang district, Sichuan 611134, PR China. Electronic address: abtclxw@126.com.
[Ti] Título:Rapid and sensitive detection of porcine torovirus by a reverse transcription loop-mediated isothermal amplification assay (RT-LAMP).
[So] Source:J Virol Methods;228:103-7, 2016 Feb.
[Is] ISSN:1879-0984
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Porcine torovirus (PToV) is associated with swine gastroenteritis, but its pathogenesis is uncertain because there is limited information regarding PToV due to its difficulty to adapt in vitro. This study has developed a rapid one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the detection of PToV. A set of four primers specific to six regions within the PToV's highly conserved fragment of the M gene was designed for use with the RT-LAMP assay. The RT-LAMP assay was sensitive with a detection limit of 1 × 10(1)copies/µL, which was 100-fold higher than reverse-transcription PCR. No cross-reaction was observed with other similar viruses. A total of 175 clinical specimens were collected from the Sichuan province, and PToV was detected by the established RT-LAMP assay with a positive rate of 39.2% (69/175). This study developed the first rapid, sensitive, simple, cost-effective and accurate method for the detection of PToV. The results show that the RT-LAMP assay is highly feasible in clinical settings.
[Mh] Termos MeSH primário: Técnicas de Amplificação de Ácido Nucleico/métodos
Doenças dos Suínos/virologia
Infecções por Torovirus/veterinária
Torovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Reações Cruzadas
Primers do DNA
Seres Humanos
Limite de Detecção
Técnicas de Amplificação de Ácido Nucleico/economia
RNA Viral
Transcrição Reversa
Sensibilidade e Especificidade
Suínos
Doenças dos Suínos/diagnóstico
Torovirus/genética
Infecções por Torovirus/diagnóstico
Infecções por Torovirus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers); 0 (RNA, Viral)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151128
[St] Status:MEDLINE


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[PMID]:26268320
[Au] Autor:Lojkic I; Kresic N; Simic I; Bedekovic T
[Ad] Endereço:Department of Virology, Croatian Veterinary Institute, Savska cesta 143, 10000, Zagreb, Croatia. ilojkic@veinst.hr.
[Ti] Título:Detection and molecular characterisation of bovine corona and toroviruses from Croatian cattle.
[So] Source:BMC Vet Res;11:202, 2015 Aug 13.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bovine coronavirus (BCoV) together with bovine torovirus (BToV), both members of the Coronaviridae family, order Nidovirales are the most common viral enteric pathogens. Although studied separately, their joint occurrence and the molecular diversity in cattle in Croatia have not been investigated. METHODS: A survey is carried out on 101 fecal samples from diarrheic young and adult cattle during the 3-year period from i) one large dairy herd, ii) four small herds and iii) three nasal and paired fecal samples from calves with symptoms of respiratory disease. Samples were submitted to RT-PCR and sequencing for BCoV Nucleocapsid gene, BCoV Spike gene and BToV Spike gene. RESULTS: BCoV was detected in 78.8 % of fecal samples from symptomatic cattle and three nasal and paired fecal samples from calves with respiratory symptoms. BToV was detected in 43.2 % of fecal samples from symptomatic cattle and a fecal sample from calves with respiratory symptoms. Molecular characterisation of those viruses revealed some nucleotide and aminoacid differences in relation to reference strains. CONCLUSIONS: BToV should be regarded as a relevant pathogen for cattle that plays a synergistic role in mixed enteric infections.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Infecções por Coronavirus/veterinária
Coronavirus Bovino/isolamento & purificação
Infecções por Torovirus/veterinária
Torovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/epidemiologia
Infecções por Coronavirus/epidemiologia
Infecções por Coronavirus/virologia
Coronavirus Bovino/genética
Croácia/epidemiologia
DNA Complementar/genética
DNA Viral/genética
Diarreia/epidemiologia
Diarreia/veterinária
Diarreia/virologia
Fezes/virologia
Filogenia
Alinhamento de Sequência
Torovirus/genética
Infecções por Torovirus/epidemiologia
Infecções por Torovirus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (DNA, Viral)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150815
[Lr] Data última revisão:
150815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150814
[St] Status:MEDLINE
[do] DOI:10.1186/s12917-015-0511-9


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[PMID]:24903213
[Au] Autor:Zhou L; Wei H; Zhou Y; Xu Z; Zhu L; Horne J
[Ti] Título:Molecular epidemiology of Porcine torovirus (PToV) in Sichuan Province, China: 2011-2013.
[So] Source:Virol J;11:106, 2014 Jun 05.
[Is] ISSN:1743-422X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Porcine torovirus (PToV) is a member of the genus Torovirus which is responsible for gastrointestinal disease in both human beings and animals with particular prevalence in youth. Torovirus infections are generally asymptomatic, however, their presence may worsen disease consequences in concurrent infections with other enteric pathogens. METHODS: A total of 872 diarrheic fecal samples from pigs of different ages were collected from 12 districts of Sichuan Province in the southwest of China. RT-PCR was done with PToV S gene specific primers to detect the presence of PToV positive samples. M gene specific primers were used with the PToV positive samples and the genes were sequenced. A phylogenetic tree was constructed based on the M gene nucleotide sequences from the 19 selected novel Sichuan strains and 21 PToV and BToV M gene sequences from GenBank. RESULTS: A total of 331 (37.96%, 331/872) samples were found to be positive for PToV and the highest prevalence was observed in piglets aged from 1 to 3 weeks old. Through phylogenetic inference the 40 PToV M gene containing sequences were placed into two genotypes (I & II). The 19 novel Sichuan strains of genotype I showed strong correlations to two Korean gene sequences (GU-07-56-11 and GU-07-56-22). Amino-acid sequence analysis of the 40 PToV M gene strains revealed that the M gene protein was highly conserved. CONCLUSIONS: This study uncovered the presence of PToV in Sichuan Province, and demonstrated the need for continuous surveillance PToV of epidemiology.
[Mh] Termos MeSH primário: Doenças dos Suínos/epidemiologia
Doenças dos Suínos/virologia
Infecções por Torovirus/veterinária
Torovirus/classificação
Torovirus/genética
[Mh] Termos MeSH secundário: Animais
China/epidemiologia
Análise por Conglomerados
Diarreia/epidemiologia
Diarreia/veterinária
Diarreia/virologia
Fezes/virologia
Epidemiologia Molecular
Dados de Sequência Molecular
Filogenia
RNA Viral/genética
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Análise de Sequência de DNA
Homologia de Sequência
Suínos
Torovirus/isolamento & purificação
Infecções por Torovirus/epidemiologia
Infecções por Torovirus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Viral)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:150805
[Lr] Data última revisão:
150805
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140607
[St] Status:MEDLINE
[do] DOI:10.1186/1743-422X-11-106


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[PMID]:24420162
[Au] Autor:Gülaçti I; Isidan H; Sözdutmaz I
[Ad] Endereço:The Pendik Veterinary Control and Research Institute, 34890, Istanbul, Turkey.
[Ti] Título:Detection of bovine torovirus in fecal specimens from calves with diarrhea in Turkey.
[So] Source:Arch Virol;159(7):1623-7, 2014 Jul.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Bovine torovirus (BToV), a member of the family Coronaviridae, is an established gastrointestinal infectious agent in cattle. In this study, we performed a survey to detect BToV in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by the nested reverse transcription (RT) PCR method using primers located in the consensus sequences of the BToV membrane (M) gene. The BToV M gene was detected in 4.7 % (11/235) of the samples using the nested RT-PCR method. The nucleotide sequences of partial M fragments from the BToV isolates, including the newly identified Turkish isolates, showed more than 96 % identity. The result indicates that BToV is one of the pathogens that contribute to neonatal calf diarrhea cases in Turkey.
[Mh] Termos MeSH primário: Doenças dos Bovinos/virologia
Diarreia/veterinária
Fezes/virologia
Infecções por Torovirus/veterinária
Torovirus/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos/epidemiologia
Diarreia/virologia
Filogenia
Torovirus/genética
Infecções por Torovirus/epidemiologia
Infecções por Torovirus/virologia
Turquia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1408
[Cu] Atualização por classe:140626
[Lr] Data última revisão:
140626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140115
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-014-1977-7


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[PMID]:24520775
[Au] Autor:Chen L; Zhu L; Zhou YC; Guo WZ
[Ad] Endereço:College of Veterinary Medicine of Sichuan Agricultural University, Ya'an 625014, China. morn_chen@126.com
[Ti] Título:[A review of porcine torovirus research: etiology and epidemiology].
[So] Source:Bing Du Xue Bao;29(6):667-72, 2013 Nov.
[Is] ISSN:1000-8721
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:Porcine Torovirus (PToV) is widely distributed in the world with high prevalence rate in swinery. Due to the high detection rate in diarrhea pigs, PToV is thought to be a potential pathogen of swine diarrhea. In recent years, epidemic outbreaks of diarrhea with high morbidity and mortality in China have caused great economic losses. Intertypic recombination events and antigenic cross-reactivity among toroviruses implies potential zoonotic transmission of PToV. The review represented the development history of PToV and made a brief summary of the features in genome and protein epidemiology and laboratory diagnosis of the PToV, and so on.
[Mh] Termos MeSH primário: Doenças dos Suínos/virologia
Infecções por Torovirus/veterinária
Torovirus/fisiologia
[Mh] Termos MeSH secundário: Animais
China/epidemiologia
Suínos
Doenças dos Suínos/epidemiologia
Torovirus/genética
Infecções por Torovirus/epidemiologia
Infecções por Torovirus/virologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Em] Mês de entrada:1404
[Cu] Atualização por classe:161021
[Lr] Data última revisão:
161021
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140214
[St] Status:MEDLINE


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[PMID]:24459455
[Au] Autor:Zhou Y; Chen L; Zhu L; Xu Z
[Ad] Endereço:Animal Biotechnology Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an 625014, China.
[Ti] Título:Molecular detection of porcine torovirus in piglets with diarrhea in southwest China.
[So] Source:ScientificWorldJournal;2013:984282, 2013.
[Is] ISSN:1537-744X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Porcine torovirus (PToV) was detected from intestinal samples of piglets with diarrhea from 20 farms in southwest China. The total prevalence of PToV was 45% (9 out of 20 farms); it was the first detection of PToV in China, and also the study analyzed the phylogenetic relationships between the Chinese PToV and PToV reference strains as well as other representative toroviruses. Genetic and phylogenetic analysis showed the existence of genetic diversity among geographically separated PToV. Statistical analysis of the PToV positive rate as well as a survey for other enteric pathogens in diarrheic pigs suggests that PToV may play a role as a causative agent of severe diarrhea in piglets.
[Mh] Termos MeSH primário: Diarreia/diagnóstico
Diarreia/virologia
Doenças dos Suínos/diagnóstico
Infecções por Torovirus/veterinária
Torovirus/genética
[Mh] Termos MeSH secundário: Animais
Genes Virais
Dados de Sequência Molecular
Filogenia
Suínos
Torovirus/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1406
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140125
[St] Status:MEDLINE
[do] DOI:10.1155/2013/984282


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[PMID]:24364900
[Au] Autor:Pignatelli J; Alonso-Padilla J; Rodríguez D
[Ti] Título:Lineage specific antigenic differences in porcine torovirus hemagglutinin-esterase (PToV-HE) protein.
[So] Source:Vet Res;44:126, 2013 Dec 23.
[Is] ISSN:1297-9716
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hemagglutinin-esterases (HE) are viral envelope proteins present in some members from the toro-, corona- and orthomyxovirus families, all related with enteric and/or respiratory tract infections. HE proteins mediate reversible binding to sialic acid receptor determinants, very abundant glycan residues in the enteric and respiratory tracts. The role of the HE protein during the torovirus infection cycle remains unknown, although it is believed to be important in the natural infection process. The phylogenetic analysis of HE coding sequences from porcine torovirus (PToV) field strains revealed the existence of two distinct HE lineages. In a previous study, PToV virus strains with HE proteins from the two lineages were found coexisting in a pig herd, and they were even obtained from the same animal at two consecutive sampling time points. In this work, we report antigenic differences between the two HE lineages, and discuss the possible implications that the coexistence of viruses belonging to both lineages might have on the spread and sustainment of PToV infection in the farms.
[Mh] Termos MeSH primário: Hemaglutininas Virais/genética
Doenças dos Suínos/imunologia
Infecções por Torovirus/veterinária
Torovirus/enzimologia
Proteínas Virais de Fusão/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Antígenos Virais/sangue
Antígenos Virais/genética
Hemaglutininas Virais/metabolismo
Dados de Sequência Molecular
Filogenia
Alinhamento de Sequência/veterinária
Suínos
Doenças dos Suínos/virologia
Torovirus/classificação
Infecções por Torovirus/imunologia
Infecções por Torovirus/virologia
Proteínas Virais de Fusão/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, Viral); 0 (Hemagglutinins, Viral); 0 (Viral Fusion Proteins); 0 (hemagglutinin esterase)
[Em] Mês de entrada:1407
[Cu] Atualização por classe:150422
[Lr] Data última revisão:
150422
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131225
[St] Status:MEDLINE
[do] DOI:10.1186/1297-9716-44-126


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[PMID]:23749172
[Au] Autor:Cong Y; Zarlenga DS; Richt JA; Wang X; Wang Y; Suo S; Wang J; Ren Y; Ren X
[Ad] Endereço:College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.
[Ti] Título:Evolution and homologous recombination of the hemagglutinin-esterase gene sequences from porcine torovirus.
[So] Source:Virus Genes;47(1):66-74, 2013 Aug.
[Is] ISSN:1572-994X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of the present study was to gain new insights into the evolution, homologous recombination, and selection pressures imposed on the porcine torovirus (PToV), by examining the changes in the hemagglutinin-esterase (HE) gene. The most recent common ancestor of PToV was estimated to have emerged 62 years ago based upon HE gene sequence data obtained from PToV isolates originating from Spain, South Korea, Netherlands, Hungary, and Italy and using the HE gene of Bovine torovirus isolates Niigata1 (AB661456) and Niigata3 (AB661458) as outgroups. The HE gene sequence data segregated all the PToV isolates into two well-supported monophyletic groups; however, various isolates from Spain, Italy, and South Korea did not segregate geographically suggesting very recent translocation of the viruses to these localities. Evidence of recombination was observed between two South Korean isolates that partitioned into two distinct subclades. Data further suggest that most of the nucleotides in the HE gene are under negative selection; however, changes within codon 237 showed an evidence of positive selection.
[Mh] Termos MeSH primário: Evolução Molecular
Hemaglutininas Virais/genética
Recombinação Homóloga
Doenças dos Suínos/virologia
Infecções por Torovirus/veterinária
Torovirus/genética
Proteínas Virais de Fusão/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Hemaglutininas Virais/química
Itália
Dados de Sequência Molecular
Países Baixos
Conformação de Ácido Nucleico
Filogenia
República da Coreia
Seleção Genética
Espanha
Suínos
Torovirus/química
Torovirus/classificação
Infecções por Torovirus/virologia
Proteínas Virais de Fusão/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Hemagglutinins, Viral); 0 (Viral Fusion Proteins); 0 (hemagglutinin esterase)
[Em] Mês de entrada:1402
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130611
[St] Status:MEDLINE
[do] DOI:10.1007/s11262-013-0926-y


  10 / 42 MEDLINE  
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Richtzenhain, Leonardo José
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[PMID]:23648077
[Au] Autor:Nogueira JS; Asano KM; de Souza SP; Brandão PE; Richtzenhain LJ
[Ad] Endereço:Department of Preventive Veterinary Medicine and Animal Health, College of Veterinary Medicine, University of São Paulo. Av. Prof. Dr. Orlando Marques de Paiva, 87, CEP 05508-270, Cidade Universitária, São Paulo, Brazil. js.nogueira@yahoo.com.br
[Ti] Título:First detection and molecular diversity of Brazilian bovine torovirus (BToV) strains from young and adult cattle.
[So] Source:Res Vet Sci;95(2):799-801, 2013 Oct.
[Is] ISSN:1532-2661
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bovine torovirus (BToV) is an established enteric pathogen of cattle, but its occurrence in Brazilian cattle had not been reported until now. This article describes a survey on BToV in Brazil carried out on 80 fecal samples from diarrheic young and adult cattle, using a nested-RT-PCR targeting the nucleocapsid (N) gene. BToV was detected in 6.25% (5/80) of stool samples from three different geographic regions. Sequences analysis showed that Brazilian BToVs have a high degree of identity with European and Japanese BToVs and a lower degree of identity with North American Breda 1 strain. These results show that, albeit its low frequency and the scarce number of research on the field, BToV is still present amongst cattle populations.
[Mh] Termos MeSH primário: Variação Genética
Infecções por Torovirus/veterinária
Torovirus/classificação
[Mh] Termos MeSH secundário: Animais
Brasil/epidemiologia
Bovinos
Europa (Continente)/epidemiologia
Japão/epidemiologia
Filogenia
Torovirus/genética
Infecções por Torovirus/epidemiologia
Infecções por Torovirus/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1404
[Cu] Atualização por classe:130812
[Lr] Data última revisão:
130812
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130508
[St] Status:MEDLINE



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