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[PMID]:29292957
[Au] Autor:Bjerin O; Martín Muñoz D; Gerald C; Brytting M; Eriksson M
[Ad] Endereço:Karolinska sjukhuset - Solna, Sweden - pediatric neurology Solna, Sweden.
[Ti] Título:Misstänkt koppling mellan enterovirus D68 och akut slapp myelit hos svenska barn - Ansamling av fall under några veckor hösten 2016..
[So] Source:Lakartidningen;114, 2017 Nov 30.
[Is] ISSN:1652-7518
[Cp] País de publicação:Sweden
[La] Idioma:swe
[Ab] Resumo:Acute flaccid myelitis amongst Swedish children with a possible link to an outbreak of enterovirus D68 In september 2016 we had several cases of acute flaccid myelitis in our clinic. This coincided with an outbreak of enterovirus D68 (EV-D68) in Sweden during the same period. We describe three cases, of which one tested positive for EV-D68. Acute flaccid paralysis of one or more limbs preceded by an upper respiratory tract infection is highly suspicious of myelitis, and a viral cause must be included in the clinical work-up. In order to detect infection with EV-D68 in suspected acute flaccid myelitis, nasopharyngeal aspirate should be performed as early as possible. EV-D68 is normally not found in stool or cerebrospinal fluid tests but should be included in the clinical work-up. Treatment of acute flaccid myelitis is supportive only. There is no effective antiviral treatment and immunomodulating therapies show little effect. Persisting neurological deficits are common but lethal cases are rare.
[Mh] Termos MeSH primário: Infecções por Enterovirus/complicações
Mielite/virologia
Paralisia/virologia
[Mh] Termos MeSH secundário: Doença Aguda
Criança
Pré-Escolar
Surtos de Doenças
Enterovirus Humano D/isolamento & purificação
Infecções por Enterovirus/epidemiologia
Infecções por Enterovirus/terapia
Feminino
Seres Humanos
Imagem por Ressonância Magnética
Masculino
Mielite/diagnóstico por imagem
Mielite/epidemiologia
Mielite/terapia
Paralisia/epidemiologia
Paralisia/terapia
Suécia/epidemiologia
[Pt] Tipo de publicação:CASE REPORTS
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE


  2 / 4207 MEDLINE  
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[PMID]:29424218
[Au] Autor:Sergevnin VI; Tryasolobova MA; Kudrevatykh EV; Kuzovnikova EZ
[Ti] Título:[The frequency of detection of non-polio enteroviruses in foul and fecal waste waters, water and some food products].
[So] Source:Gig Sanit;95(6):525-8, 2016.
[Is] ISSN:0016-9900
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:In the Perm Territory from 2010 to 2014 155 samples offoul andfecal waste waters, 293 samples of surface water, 827 samples of supply net water, and 57 vegetable and fruit water-washes were examined for the RNA enterovirus agent with the use of polymerase chain reaction (PCR) method. In parallel 155 wastewater samples, 20 samples of surface water, and 4 samples of supply net water were examined for non-polio enterovirus agent with the use of virological methods. In the samples of foul waste waters the RNA enterovirus agent was detected in 74.8 ± 3.4%, and nonpolio enterovirus agent - in 65.1 ± 3.8%. In the samples of surface water the RNA enterovirus agent was detected in 2.3 ± 0.8%; in the area offoul and fecal waste waters the non-polio enterovirus agent was detected in 20.0 ± 4.4% in the process of virological investigation of RNA-positive water samples. In supply net water the RNA enterovirus agent was detected in 0.8 ± 0.3 %, on the surface of vegetables, fruits, and grapes - in 10.5 ± 3.9 %.
[Mh] Termos MeSH primário: Infecções por Enterovirus
Enterovirus
Água Doce
Frutas/virologia
Verduras/virologia
Águas Residuais/virologia
[Mh] Termos MeSH secundário: Enterovirus/isolamento & purificação
Enterovirus/patogenicidade
Infecções por Enterovirus/epidemiologia
Infecções por Enterovirus/prevenção & controle
Infecções por Enterovirus/virologia
Monitoramento Ambiental/métodos
Água Doce/análise
Água Doce/virologia
Seres Humanos
Saúde Pública/métodos
Saúde Pública/normas
Saúde Pública/estatística & dados numéricos
Federação Russa/epidemiologia
Eliminação de Resíduos Líquidos/normas
Abastecimento de Água/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180210
[St] Status:MEDLINE


  3 / 4207 MEDLINE  
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[PMID]:28469998
[Au] Autor:Yang X; Xie J; Jia L; Liu N; Liang Y; Wu F; Liang B; Li Y; Wang J; Sheng C; Li H; Liu H; Ma Q; Yang C; Du X; Qiu S; Song H
[Ad] Endereço:Center for Infectious Disease Control, Institute of Disease Control and Prevention, Academy of Military Medical SciencesBeijing, China.
[Ti] Título:Analysis of miRNAs Involved in Mouse Brain Damage upon Enterovirus 71 Infection.
[So] Source:Front Cell Infect Microbiol;7:133, 2017.
[Is] ISSN:2235-2988
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Enterovirus 71 (EV71) infects the central nervous system (CNS) and causes brainstem encephalitis in children. MiRNAs have been found to play various functions in EV71 infection in human cell lines. To identify potential miRNAs involved in the inflammatory injury in CNS, our study, for the first time, performed a miRNA microarray assay using EV71 infected mice brains. Twenty differentially expressed miRNAs were identified (four up- and 16 down-regulated) and confirmed by qRT-PCR. The target genes of these miRNAs were analyzed using KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis, revealing that the miRNAs were mainly involved in the regulation of inflammation and neural system function. MiR-150-5p, -3082-5p, -3473a, -468-3p, -669n, -721, -709, and -5107-5p that regulate MAPK and chemokine signaling were all down-regulated, which might result in increased cytokine production. In addition, miR-3473a could also regulate focal adhesion and leukocyte trans-endothelial migration, suggesting a role in virus-induced blood-brain barrier disruption. The miRNAs and pathways identified in this study could help to understand the intricate interactions between EV71 and the brain injury, offering new insight for the future research of the molecular mechanism of EV71 induced brainstem encephalitis.
[Mh] Termos MeSH primário: Lesões Encefálicas/patologia
Encéfalo/virologia
Enterovirus Humano A/patogenicidade
MicroRNAs/metabolismo
MicroRNAs/farmacologia
[Mh] Termos MeSH secundário: Animais
Barreira Hematoencefálica
Encéfalo/patologia
Lesões Encefálicas/virologia
Linhagem Celular
Sistema Nervoso Central/patologia
Sistema Nervoso Central/virologia
Quimiocinas/metabolismo
Citocinas/metabolismo
Regulação para Baixo
Enterovirus Humano A/genética
Infecções por Enterovirus
Perfilação da Expressão Gênica
Seres Humanos
Inflamação
Camundongos
MicroRNAs/genética
Quinases de Proteína Quinase Ativadas por Mitógeno/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Taxa de Sobrevida
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Chemokines); 0 (Cytokines); 0 (MicroRNAs); EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.3389/fcimb.2017.00133


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[PMID]:28455155
[Au] Autor:Korematsu S; Nagashima K; Sato Y; Nagao M; Hasegawa S; Nakamura H; Sugiura S; Miura K; Okada K; Fujisawa T
[Ad] Endereço:Department of Pediatrics, Oita University Faculty of Medicine, Oita, Japan.
[Ti] Título:"Spike" in acute asthma exacerbations during enterovirus D68 epidemic in Japan: A nation-wide survey.
[So] Source:Allergol Int;67(1):55-60, 2018 Jan.
[Is] ISSN:1440-1592
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: In September 2015, Japan experienced an unusual increase in acute asthma hospitalizations of children that coincided with an enterovirus D68 (EV-D68) epidemic. The objective of this study is to investigate whether EV-D68 had a causal relationship with the spike in asthma hospitalizations. METHODS: A nation-wide retrospective survey of asthma hospitalizations of children was performed for the period from January 2010 through October 2015. The Japanese Society of Pediatric Allergy and Clinical Immunology asked its affiliated hospitals to report monthly numbers of hospitalizations, ICU admissions and mechanical ventilations due to acute asthma exacerbation. The data were retrieved from medical databases using predefined search criteria: diagnosis of asthma or asthmatic bronchitis, admission, and age <20 years. Monthly numbers of EV-D68 detection were also obtained from the Infectious Disease Surveillance Center of Japan. A Granger causality test was used to analyze the association of EV-D68 detections for asthma exacerbation. RESULTS: A total of 157 hospitals reported 87,189 asthma hospitalizations, including 477 ICU admissions and 1193 mechanical ventilations, during the survey period of 5 years and 10 months. The numbers of these events increased drastically in September 2015. The Granger causality test verified the association between EV-D68 and asthma hospitalizations/mechanical ventilations. The most-affected age group was 3-6 years old. CONCLUSIONS: The spike in pediatric asthma hospitalizations in Japan in September 2015 was found to be associated with the EV-D68 epidemic. Respiratory pathogens can cause "epidemics" of asthma exacerbation. Coordinated surveillance of infectious diseases and asthma may be beneficial for prevention and better control of both illnesses.
[Mh] Termos MeSH primário: Asma/epidemiologia
Enterovirus Humano D
Infecções por Enterovirus/epidemiologia
Epidemias
Hospitalização
[Mh] Termos MeSH secundário: Doença Aguda
Adolescente
Adulto
Asma/terapia
Criança
Pré-Escolar
Infecções por Enterovirus/terapia
Feminino
Seres Humanos
Lactente
Recém-Nascido
Japão/epidemiologia
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180210
[Lr] Data última revisão:
180210
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170430
[St] Status:MEDLINE


  5 / 4207 MEDLINE  
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[PMID]:29324778
[Au] Autor:Tsuchiaka S; Naoi Y; Imai R; Masuda T; Ito M; Akagami M; Ouchi Y; Ishii K; Sakaguchi S; Omatsu T; Katayama Y; Oba M; Shirai J; Satani Y; Takashima Y; Taniguchi Y; Takasu M; Madarame H; Sunaga F; Aoki H; Makino S; Mizutani T; Nagai M
[Ad] Endereço:Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
[Ti] Título:Genetic diversity and recombination of enterovirus G strains in Japanese pigs: High prevalence of strains carrying a papain-like cysteine protease sequence in the enterovirus G population.
[So] Source:PLoS One;13(1):e0190819, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To study the genetic diversity of enterovirus G (EV-G) among Japanese pigs, metagenomics sequencing was performed on fecal samples from pigs with or without diarrhea, collected between 2014 and 2016. Fifty-nine EV-G sequences, which were >5,000 nucleotides long, were obtained. By complete VP1 sequence analysis, Japanese EV-G isolates were classified into G1 (17 strains), G2 (four strains), G3 (22 strains), G4 (two strains), G6 (two strains), G9 (six strains), G10 (five strains), and a new genotype (one strain). Remarkably, 16 G1 and one G2 strain identified in diarrheic (23.5%; four strains) or normal (76.5%; 13 strains) fecal samples possessed a papain-like cysteine protease (PL-CP) sequence, which was recently found in the USA and Belgium in the EV-G genome, at the 2C-3A junction site. This paper presents the first report of the high prevalence of viruses carrying PL-CP in the EV-G population. Furthermore, possible inter- and intragenotype recombination events were found among EV-G strains, including G1-PL-CP strains. Our findings may advance the understanding of the molecular epidemiology and genetic evolution of EV-Gs.
[Mh] Termos MeSH primário: Infecções por Enterovirus/virologia
Enterovirus Suínos/genética
Variação Genética
Recombinação Genética
[Mh] Termos MeSH secundário: Animais
Proteínas do Capsídeo/genética
Cisteína Proteases/genética
Infecções por Enterovirus/epidemiologia
Enterovirus Suínos/enzimologia
Fezes/virologia
Japão
Metagenoma
Filogenia
Prevalência
Sus scrofa
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Capsid Proteins); EC 3.4.- (Cysteine Proteases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190819


  6 / 4207 MEDLINE  
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[PMID]:27771182
[Au] Autor:Yang L; Liu Y; Li S; Zhao H; Lin Q; Yu H; Huang X; Zheng Q; Cheng T; Xia N
[Ad] Endereço:State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Science & School of Public Health, Xiamen University, Xiamen, China.
[Ti] Título:A novel inactivated enterovirus 71 vaccine can elicit cross-protective immunity against coxsackievirus A16 in mice.
[So] Source:Vaccine;34(48):5938-5945, 2016 11 21.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Hand, foot, and mouth disease (HFMD) is a highly contagious disease that mainly affects infants and children. Enterovirus 71 (EV71) and coxsackievirus A16 (CA16) are the major pathogens of HFMD. Two EV71 vaccines were recently licensed in China and the administration of the EV71 vaccines is believed to significantly reduce the number of HFMD-related severe or fatal cases. However, a monovalent EV71 vaccine cannot cross-protect against CA16 infection, this may result in that it cannot effectively control the overall HFMD epidemic. In this study, a chimeric EV71, whose VP1/210-225 epitope was replaced by that of CA16, was constructed using a reverse genetics technique to produce a candidate EV71/CA16 bivalent vaccine strain. The chimeric EV71 was infectious and showed similar growth characteristics as its parental strain. The replacement of the VP1/210-225 epitope did not significantly affect the antigenicity and immunogenicity of EV71. More importantly, the chimeric EV71 could induce protective immunity against both EV71 and CA16, and protect neonatal mice against either EV71 or CA16 lethal infections, the chimeric EV71 constructed in this study was shown to be a feasible and promising candidate bivalent vaccine against both EV71 and CA16. The construction of a chimeric enterovirus also provides an alternative platform for broad-spectrum HFMD vaccines development.
[Mh] Termos MeSH primário: Infecções por Coxsackievirus/prevenção & controle
Proteção Cruzada
Infecções por Enterovirus/prevenção & controle
Enterovirus/imunologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Neutralizantes/sangue
Anticorpos Antivirais/sangue
Infecções por Coxsackievirus/imunologia
Enterovirus Humano A/genética
Enterovirus Humano A/imunologia
Infecções por Enterovirus/imunologia
Epitopos/imunologia
Doença de Mão, Pé e Boca/prevenção & controle
Seres Humanos
Imunogenicidade da Vacina
Camundongos
Genética Reversa
Vacinas de Produtos Inativados/administração & dosagem
Vacinas de Produtos Inativados/imunologia
Vacinas Virais/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Epitopes); 0 (Vaccines, Inactivated); 0 (Viral Vaccines)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  7 / 4207 MEDLINE  
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[PMID]:27771495
[Au] Autor:Karrasch M; Fischer E; Scholten M; Sauerbrei A; Henke A; Renz DM; Mentzel HJ; Böer K; Böttcher S; Diedrich S; Krumbholz A; Zell R
[Ad] Endereço:Institute of Medical Microbiology, Jena University Hospital, Jena, Germany. Electronic address: matthias.karrasch@med.uni-jena.de.
[Ti] Título:A severe pediatric infection with a novel enterovirus A71 strain, Thuringia, Germany.
[So] Source:J Clin Virol;84:90-95, 2016 11.
[Is] ISSN:1873-5967
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Infection by Enterovirus A71 (EV-A71) is an important cause of hand, foot, and mouth disease (HFMD). Outbreaks including severe cases with neurological and cardiopulmonary complications have been reported particularly from Southeast Asia. In Europe, the epidemiology of EV-A71 is not well understood. In summer 2015, a two-year-old girl from Thuringia, Germany, presented with rhombencephalitis/brainstem encephalitis associated with severe neurological and cardiopulmonary complications. EV-A71 was detected in stool and almost the entire viral genome was amplified and sequenced. While the capsid protein VP1-encoding region belongs to the EV-A71 subgenogroup C1, the 3D polymerase encoding region represents a unique lineage. Thus, the data suggest that the Thuringian EV-A71 sequence likely represents a recombinant. The case underlines the importance of intensified EV-A71 surveillance in Germany and Europe including analysis of full-genome data.
[Mh] Termos MeSH primário: Encefalite Viral/virologia
Enterovirus Humano A/isolamento & purificação
Infecções por Enterovirus/virologia
[Mh] Termos MeSH secundário: Proteínas do Capsídeo/genética
Pré-Escolar
Surtos de Doenças
Encefalite Viral/diagnóstico
Encefalite Viral/epidemiologia
Enterovirus Humano A/classificação
Enterovirus Humano A/genética
Enterovirus Humano A/patogenicidade
Infecções por Enterovirus/diagnóstico
Infecções por Enterovirus/epidemiologia
Fezes/virologia
Feminino
Genoma Viral
Alemanha/epidemiologia
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Recombinação Genética
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Capsid Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  8 / 4207 MEDLINE  
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[PMID]:28938017
[Au] Autor:Zhang YX; Huang YM; Li QJ; Li XY; Zhou YD; Guo F; Zhou JM; Cen S
[Ad] Endereço:Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical School, Beijing, PR China.
[Ti] Título:A highly conserved amino acid in VP1 regulates maturation of enterovirus 71.
[So] Source:PLoS Pathog;13(9):e1006625, 2017 Sep.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Enterovirus 71 (EV71) is the major causative agent of hand, foot and mouth disease (HFMD) in children, causing severe clinical outcomes and even death. Here, we report an important role of the highly conserved alanine residue at position 107 in the capsid protein VP1 (VP1A107) in the efficient replication of EV71. Substitutional mutations of VP1A107 significantly diminish viral growth kinetics without significant effect on viral entry, expression of viral genes and viral production. The results of mechanistic studies reveal that VP1A107 regulates the efficient cleavage of the VP0 precursor during EV71 assembly, which is required, in the next round of infection, for the transformation of the mature virion (160S) into an intermediate or A-particle (135S), a key step of virus uncoating. Furthermore, the results of molecular dynamic simulations and hydrogen-bond networks analysis of VP1A107 suggest that flexibility of the VP1 BC loop or the region surrounding the VP1107 residue directly correlates with viral infectivity. It is possible that sufficient flexibility of the region surrounding the VP1107 residue favors VP0 conformational change that is required for the efficient cleavage of VP0 as well as subsequent viral uncoating and viral replication. Taken together, our data reveal the structural role of the highly conserved VP1A107 in regulating EV71 maturation. Characterization of this novel determinant of EV71 virulence would promote the study on pathogenesis of Enteroviruses.
[Mh] Termos MeSH primário: Enterovirus Humano A/fisiologia
Infecções por Enterovirus/virologia
Células Vero/virologia
Replicação Viral/genética
[Mh] Termos MeSH secundário: Aminoácidos/genética
Animais
Proteínas do Capsídeo/metabolismo
Cercopithecus aethiops
Mutação de Sentido Incorreto/genética
Internalização do Vírus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Capsid Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006625


  9 / 4207 MEDLINE  
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[PMID]:28902862
[Au] Autor:Kaida A; Iritani N; Yamamoto SP; Kanbayashi D; Hirai Y; Togawa M; Amo K; Kohdera U; Nishigaki T; Shiomi M; Asai S; Kageyama T; Kubo H
[Ad] Endereço:Division of Microbiology, Osaka Institute of Public Health, Osaka, Japan.
[Ti] Título:Distinct genetic clades of enterovirus D68 detected in 2010, 2013, and 2015 in Osaka City, Japan.
[So] Source:PLoS One;12(9):e0184335, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The first upsurge of enterovirus D68 (EV-D68), a causative agent of acute respiratory infections (ARIs), in Japan was reported in Osaka City in 2010. In this study, which began in 2010, we surveyed EV-D68 in children with ARIs and analyzed sequences of EV-D68 strains detected. Real-time PCR of 19 respiratory viruses or subtypes of viruses, including enterovirus, was performed on 2,215 specimens from ARI patients (<10 years of age) collected between November 2010 and December 2015 in Osaka City, Japan. EV-D68 was identified in 18 enterovirus-positive specimens (n = 4 in 2013, n = 1 in 2014, and n = 13 in 2015) by analysis of viral protein 1 (VP1) or VP4 sequences, followed by a BLAST search for similar sequences. All EV-D68 strains were detected between June and October (summer to autumn), except for one strain detected in 2014. A phylogenetic analysis of available VP1 sequences revealed that the Osaka strains detected in 2010, 2013, and 2015 belonged to distinct clusters (Clades C, A, and B [Subclade B3], respectively). Comparison of the 5' untranslated regions of these viruses showed that Osaka strains in Clades A, B (Subclade B3), and C commonly had deletions at nucleotide positions 681-703 corresponding to the prototype Fermon strain. Clades B and C had deletions from nucleotide positions 713-724. Since the EV-D68 epidemic in 2010, EV-D68 re-emerged in Osaka City, Japan, in 2013 and 2015. Results of this study indicate that distinct clades of EV-D68 contributed to re-emergences of this virus in 2010, 2013, and 2015 in this limited region.
[Mh] Termos MeSH primário: Enterovirus Humano D/classificação
Enterovirus Humano D/genética
Infecções por Enterovirus/epidemiologia
Infecções por Enterovirus/virologia
[Mh] Termos MeSH secundário: Criança
Pré-Escolar
Doenças Transmissíveis Emergentes/virologia
Surtos de Doenças/estatística & dados numéricos
Feminino
Seres Humanos
Lactente
Recém-Nascido
Japão/epidemiologia
Masculino
Filogenia
Infecções Respiratórias/virologia
Análise de Sequência de DNA
Urbanização
Proteínas Estruturais Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Structural Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184335


  10 / 4207 MEDLINE  
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[PMID]:28870395
[Au] Autor:Dobrikov GM; Slavchev I; Nikolova I; Stoyanova A; Nikolova N; Mukova L; Nikolova R; Shivachev B; Galabov AS
[Ad] Endereço:Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, bl. 9, Acad. G. Bonchev str., Sofia 1113, Bulgaria. Electronic address: gmdob@orgchm.bas.bg.
[Ti] Título:Synthesis and anti-enterovirus activity of new analogues of MDL-860.
[So] Source:Bioorg Med Chem Lett;27(19):4540-4543, 2017 10 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A series of twelve novel compounds, analogues of antiviral agent MDL-860 were synthesized and their antiviral activity was evaluated in vitro against enteroviruses poliovirus 1 (PV1), Coxsackieviruses B1 (CVB1) and Coxsackieviruses B3 (CVB3). Compounds 14, 24 and 25 manifested strong antiviral effects against CVB1 and PV1 (SI values of 405 and 118 for CVB1 and PV1 respectively). In contrast to the wide anti-enteroviral activity of MDL-860, these three compounds were inactive against CVB3. Compounds 14, 24 and 25 along with MDL-860 were tested in vivo in mice infected with CVB1. Marked protective effects of compounds 14 and 24 were established, PI values of 50% and 33.3%, respectively. In addition, almost all of the tested compounds manifested very low toxicity.
[Mh] Termos MeSH primário: Antivirais/farmacologia
Infecções por Enterovirus/tratamento farmacológico
Enterovirus/efeitos dos fármacos
Nitrilos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antivirais/síntese química
Antivirais/química
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Seres Humanos
Camundongos
Testes de Sensibilidade Microbiana
Estrutura Molecular
Nitrilos/síntese química
Nitrilos/química
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Nitriles); 78940-62-2 (2-(3,4-dichlorophenoxy)-5-nitrobenzonitrile)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE



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