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[PMID]:28934316
[Au] Autor:Miller C; Boegler K; Carver S; MacMillan M; Bielefeldt-Ohmann H; VandeWoude S
[Ad] Endereço:Department of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado.
[Ti] Título:Pathogenesis of oral FIV infection.
[So] Source:PLoS One;12(9):e0185138, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Feline immunodeficiency virus (FIV) is the feline analogue of human immunodeficiency virus (HIV) and features many hallmarks of HIV infection and pathogenesis, including the development of concurrent oral lesions. While HIV is typically transmitted via parenteral transmucosal contact, recent studies prove that oral transmission can occur, and that saliva from infected individuals contains significant amounts of HIV RNA and DNA. While it is accepted that FIV is primarily transmitted by biting, few studies have evaluated FIV oral infection kinetics and transmission mechanisms over the last 20 years. Modern quantitative analyses applied to natural FIV oral infection could significantly further our understanding of lentiviral oral disease and transmission. We therefore characterized FIV salivary viral kinetics and antibody secretions to more fully document oral viral pathogenesis. Our results demonstrate that: (i) saliva of FIV-infected cats contains infectious virus particles, FIV viral RNA at levels equivalent to circulation, and lower but significant amounts of FIV proviral DNA; (ii) the ratio of FIV RNA to DNA is significantly higher in saliva than in circulation; (iii) FIV viral load in oral lymphoid tissues (tonsil, lymph nodes) is significantly higher than mucosal tissues (buccal mucosa, salivary gland, tongue); (iv) salivary IgG antibodies increase significantly over time in FIV-infected cats, while salivary IgA levels remain static; and, (v) saliva from naïve Specific Pathogen Free cats inhibits FIV growth in vitro. Collectively, these results suggest that oral lymphoid tissues serve as a site for enhanced FIV replication, resulting in accumulation of FIV particles and FIV-infected cells in saliva. Failure to induce a virus-specific oral mucosal antibody response, and/or viral capability to overcome inhibitory components in saliva may perpetuate chronic oral cavity infection. Based upon these findings, we propose a model of oral FIV pathogenesis and suggest alternative diagnostic modalities and translational approaches to study oral HIV infection.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida Felina/etiologia
Vírus da Imunodeficiência Felina/fisiologia
Boca/virologia
Saliva/virologia
[Mh] Termos MeSH secundário: Animais
Especificidade de Anticorpos
Gatos
DNA Viral/sangue
DNA Viral/metabolismo
Síndrome de Imunodeficiência Adquirida Felina/sangue
Síndrome de Imunodeficiência Adquirida Felina/transmissão
Vírus da Imunodeficiência Felina/imunologia
Imunoglobulina A/imunologia
RNA Viral/sangue
RNA Viral/metabolismo
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Immunoglobulin A); 0 (RNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185138


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[PMID]:28918382
[Au] Autor:Stavisky J; Dean RS; Molloy MH
[Ad] Endereço:Centre for Evidence-based Veterinary Medicine, School of Veterinary Medicine and Science, The University of Nottingham, Loughborough, UK.
[Ti] Título:Prevalence of and risk factors for FIV and FeLV infection in two shelters in the United Kingdom (2011-2012).
[So] Source:Vet Rec;181(17):451, 2017 Oct 28.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aims of this study were to determine the prevalence of feline leukaemia virus (FeLV) and feline immunodeficiency virus (FIV) infections in cats presented to two RSPCA (Royal Society for the Prevention of Cruelty to Animals) animal rehoming centres and to identify risk factors for infection. All cats presented at each centre between August 2011 and August 2012 were subjected to a patient-side test for FeLV/FIV on entry. Kittens under three months and cats euthanased within a short time of presentation were excluded from the study. Univariable and multivariable logistic regression were used to separately determine risk factors for FeLV and FIV infections. At shelter A, the prevalence of FIV infection was 11.4 per cent (54/474) and FeLV infection was 3 per cent (14/473), with two FIV/FeLV coinfections identified. At shelter B, the prevalence of FIV infection was 3 per cent (4/135) and FeLV infection was 0 per cent (0/135). Cats at shelter A were significantly more likely than those at shelter B to test positive for FIV (p=0.0024) and FeLV (p=0.048). Male cats were more likely to be infected with FIV (odds ratio 27.1, p=0.001), and thin body condition and musculoskeletal disease were associated with risk of FeLV. Overall, FIV-positive and FeLV-positive cats were significantly older (median ages 5.1 and 4.75 years, respectively) than the uninfected populations (median ages 3.4 and 3.5 years, respectively). This study shows that the prevalence of these diseases varies between shelter populations. Local knowledge combined with the risk factors identified may be useful in focusing resources for population testing strategies.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Abrigo para Animais/estatística & dados numéricos
Vírus da Imunodeficiência Felina/isolamento & purificação
Vírus da Leucemia Felina/isolamento & purificação
Leucemia Felina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Gatos
Instituições de Caridade
Feminino
Masculino
Prevalência
Fatores de Risco
Reino Unido/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170918
[St] Status:MEDLINE
[do] DOI:10.1136/vr.103857


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[PMID]:28780918
[Au] Autor:Sato S; Kabeya H; Negishi A; Tsujimoto H; Nishigaki K; Endo Y; Maruyama S
[Ad] Endereço:Laboratory of Veterinary Public Health, Department of Veterinary Medicine,College of Bioresource Sciences, Nihon University,1866 Kameino, Fujisawa, Kanagawa Prefecture, 252-0880,Japan.
[Ti] Título:Molecular survey of Bartonella henselae and Bartonella clarridgeiae in pet cats across Japan by species-specific nested-PCR.
[So] Source:Epidemiol Infect;145(13):2694-2700, 2017 10.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cats are known to be the main reservoir for Bartonella henselae and Bartonella clarridgeiae, which are the agents of 'cat-scratch disease' in humans. In the present study, we investigated the prevalence of the two Bartonella species on 1754 cat bloods collected from all prefectures in Japan during 2007-2008 by a nested-polymerase chain reaction (PCR) targeting the 16S-23S rRNA internal transcribed spacer region. Overall, Bartonella DNA was detected in 4·6% (80/1754) of the cats examined. The nested-PCR showed that 48·8% (39/80) of the positive cats were infected with B. henselae mono-infection, 33·8% (27/80) with B. clarridgeiae mono-infection and 17·5% (14/80) were infected with both species. The prevalence (5·9%; 65/1103) of Bartonella infection in the western part of Japan was significantly higher than that (2·3%; 15/651) of eastern Japan (P < 0·001). Statistical analysis of the cats examined suggested a significant association between Bartonella infection and FeLV infection (OR = 1·9; 95% CI = 1·1-3·4), but not with FIV infection (OR = 1·6; 95% CI = 1·0-2·6).
[Mh] Termos MeSH primário: Bartonella/isolamento & purificação
Doença da Arranhadura de Gato/veterinária
Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Leucemia Felina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Bartonella/classificação
Bartonella/genética
Bartonella henselae/classificação
Bartonella henselae/genética
Bartonella henselae/isolamento & purificação
Doença da Arranhadura de Gato/epidemiologia
Doença da Arranhadura de Gato/microbiologia
Gatos
Síndrome de Imunodeficiência Adquirida Felina/virologia
Feminino
Vírus da Imunodeficiência Felina/isolamento & purificação
Japão/epidemiologia
Vírus da Leucemia Felina/isolamento & purificação
Leucemia Felina/virologia
Masculino
Reação em Cadeia da Polimerase/veterinária
Prevalência
RNA Ribossômico/análise
RNA Viral/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal); 0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE
[do] DOI:10.1017/S0950268817001601


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[PMID]:28510253
[Au] Autor:Lacerda LC; Silva AN; Freitas JS; Cruz RDS; Said RA; Munhoz AD
[Ad] Endereço:Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
[Ti] Título:Feline immunodeficiency virus and feline leukemia virus: frequency and associated factors in cats in northeastern Brazil.
[So] Source:Genet Mol Res;16(2), 2017 May 10.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Our aims were to determine the frequencies of feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) in owned and stray cats in the northeastern region of Brazil, ascertain the status of FeLV infection, and investigate potential associated factors among the owned cats. Blood samples from 200 asymptomatic owned cats and 30 stray cats were processed using nested PCR and commercial immunochromatographic tests to diagnose infections. To evaluate the factors associated with FIV and/or FeLV in owned cats, a semi-structured interview was conducted with each owner about the animal's environment, and these data were subjected to unconditional logistic regression. The frequencies for owned cats were 6% (12/200) and 3% (6/200) for FIV and FeLV, respectively. No owned cat was positive for both viruses. Stray cats showed frequencies of 6.66% (2/30) and 0% (0/30) for FIV and FeLV, respectively. Contact with other cats and living in peri-urban areas were considered to be risk factors (P < 0.05) for FIV. We did not identify any factors associated with infections with FeLV. Our results confirm the presence of these two retroviruses in the region under study. Our use of different diagnostic techniques allowed us to determine the frequency of retroviruses in the feline population more accurately, particularly with regard to infections by FeLV, which have complex pathogenesis.
[Mh] Termos MeSH primário: Gatos/virologia
Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Vírus da Imunodeficiência Felina/genética
Vírus da Leucemia Felina/genética
Leucemia Felina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Brasil
Síndrome de Imunodeficiência Adquirida Felina/virologia
Feminino
Leucemia Felina/virologia
Masculino
Animais de Estimação/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16029633


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[PMID]:28384338
[Au] Autor:Eckstrand CD; Sparger EE; Pitt KA; Murphy BG
[Ad] Endereço:Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington, United States of America.
[Ti] Título:Peripheral and central immune cell reservoirs in tissues from asymptomatic cats chronically infected with feline immunodeficiency virus.
[So] Source:PLoS One;12(4):e0175327, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Feline immunodeficiency virus (FIV) infection in cats results in life-long viral persistence and progressive immunopathology. We have previously described a cohort of experimentally infected cats demonstrating a progressive decline of peripheral blood CD4+ T-cell over six years in the face of apparent peripheral viral latency. More recently we reported findings from this same cohort that revealed popliteal lymph node tissue as sites for ongoing viral replication suggesting that tissue reservoirs are important in FIV immunopathogenesis during the late asymptomatic phase of infection. Results reported herein characterize important tissue reservoirs of active viral replication during the late asymptomatic phase by examining biopsied specimens of spleen, mesenteric lymph node (MLN), and intestine from FIV-infected and uninfected control cats. Peripheral blood collected coincident with harvest of tissues demonstrated severe CD4+ T-cell depletion, undetectable plasma viral gag RNA and rarely detectable peripheral blood mononuclear cell (PBMC)-associated viral RNA (vRNA) by real-time PCR. However, vRNA was detectable in all three tissue sites from three of four FIV-infected cats despite the absence of detectable vRNA in plasma. A novel in situ hybridization assay identified B cell lymphoid follicular domains as microanatomical foci of ongoing FIV replication. Additionally, we demonstrated that CD4+ leukocyte depletion in tissues, and CD4+ and CD21+ leukocytes as important cellular reservoirs of ongoing replication. These findings revealed that tissue reservoirs support foci of ongoing viral replication, in spite of highly restricted viral replication in blood. Lentiviral eradication strategies will need address tissue viral reservoirs.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida Felina/imunologia
[Mh] Termos MeSH secundário: Animais
Linfócitos T CD4-Positivos/imunologia
Gatos
Doença Crônica
Progressão da Doença
Vírus da Imunodeficiência Felina/genética
Vírus da Imunodeficiência Felina/fisiologia
Depleção Linfocítica
Tecido Linfoide/imunologia
Tecido Linfoide/virologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175327


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[PMID]:27770018
[Au] Autor:Westman ME; Malik R; Hall E; Harris M; Hosie MJ; Norris JM
[Ad] Endereço:1 Sydney School of Veterinary Science, The University of Sydney, Sydney, NSW, Australia.
[Ti] Título:Duration of antibody response following vaccination against feline immunodeficiency virus.
[So] Source:J Feline Med Surg;19(10):1055-1064, 2017 Oct.
[Is] ISSN:1532-2750
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives Recently, two point-of-care (PoC) feline immunodeficiency virus (FIV) antibody test kits (Witness and Anigen Rapid) were reported as being able to differentiate FIV-vaccinated from FIV-infected cats at a single time point, irrespective of the gap between testing and last vaccination (0-7 years). The aim of the current study was to investigate systematically anti-FIV antibody production over time in response to the recommended primary FIV vaccination series. Methods First, residual plasma from the original study was tested using a laboratory-based ELISA to determine whether negative results with PoC testing were due to reduced as opposed to absent antibodies to gp40. Second, a prospective study was performed using immunologically naive client-owned kittens and cats given a primary FIV vaccination series using a commercially available inactivated whole cell/inactivated whole virus vaccine (Fel-O-Vax FIV, three subcutaneous injections at 4 week intervals) and tested systematically (up to 11 times) over 6 months, using four commercially available PoC FIV antibody kits (SNAP FIV/FeLV Combo [detects antibodies to p15/p24], Witness FeLV/FIV [gp40], Anigen Rapid FIV/FeLV [p24/gp40] and VetScan FeLV/FIV Rapid [p24]). Results The laboratory-based ELISA showed cats from the original study vaccinated within the previous 0-15 months had detectable levels of antibodies to gp40, despite testing negative with two kits that use gp40 as a capture antigen (Witness and Anigen Rapid kits). The prospective study showed that antibody testing with SNAP Combo and VetScan Rapid was positive in all cats 2 weeks after the second primary FIV vaccination, and remained positive for the duration of the study (12/12 and 10/12 cats positive, respectively). Antibody testing with Witness and Anigen Rapid was also positive in a high proportion of cats 2 weeks after the second primary FIV vaccination (8/12 and 7/12, respectively), but antibody levels declined below the level of detection in most cats (10/12) by 1 month after the third (final) primary FIV vaccination. All cats tested negative using Witness and Anigen Rapid 6 months after the third primary FIV vaccination. Conclusions and relevance This study has shown that a primary course of FIV vaccination does not interfere with FIV antibody testing in cats using Witness and Anigen Rapid, provided primary vaccination has not occurred within the previous 6 months. Consequently, Witness and Anigen Rapid antibody test kits can be used reliably to determine FIV infection status at the time of annual booster FIV vaccination to help detect 'vaccine breakthroughs' and in cats that have not received a primary course of FIV vaccination within the preceding 6 months. The duration of antibody response following annual booster FIV vaccination and the resulting effect on antibody testing using PoC kits needs to be determined by further research. The mechanism(s) for the variation in FIV antibody test kit performance remains unclear.
[Mh] Termos MeSH primário: Anticorpos Antivirais/imunologia
Doenças do Gato/imunologia
Síndrome de Imunodeficiência Adquirida Felina/imunologia
Vírus da Imunodeficiência Felina/imunologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Doenças do Gato/virologia
Gatos
Síndrome de Imunodeficiência Adquirida Felina/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Viral Vaccines)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161023
[St] Status:MEDLINE
[do] DOI:10.1177/1098612X16673292


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[PMID]:27307141
[Au] Autor:Taffin ER; Paepe D; Ghys LF; De Roover K; Van de Maele I; Saunders JH; Duchateau L; Daminet S
[Ad] Endereço:1 Department of Small Animal Medicine and Clinical Biology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
[Ti] Título:Systolic blood pressure, routine kidney variables and renal ultrasonographic findings in cats naturally infected with feline immunodeficiency virus.
[So] Source:J Feline Med Surg;19(6):672-679, 2017 Jun.
[Is] ISSN:1532-2750
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objectives Hypertension is a common cause of proteinuria in HIV-infected people. In cats, feline immunodeficiency virus (FIV) infection appears to be associated with proteinuria. Therefore, the results from systolic blood pressure (SBP) measurements in naturally infected FIV-positive cats were reviewed to assess whether hypertension contributes to the observed proteinuria in these cats. Ultrasonographic findings in FIV-positive cats were reviewed to complete renal assessment and to extend the scant knowledge on renal ultrasonography in cats. Methods Data from client-owned, naturally infected FIV-positive cats were retrospectively reviewed. To obtain a control group, records were reviewed from age-matched, privately owned, FIV-negative cats. Results Data from 91 FIV-infected and 113 control cats were compared. FIV-infected cats showed a significantly lower SBP ( P <0.0001) and significantly fewer FIV-infected cats were hypertensive (⩾160 mmHg) compared with control cats ( P = 0.025). The prevalence of renal azotaemia did not significantly differ between groups, although FIV-infected cats had significantly lower urine specific gravity (USG) ( P = 0.0273) and a higher incidence of USG below 1.035 ( P = 0.043). Urinary protein:creatinine ratio (UPC) was significantly higher in FIV-infected cats ( P = 0.0005) and proteinuria (UPC >0.4) occurred more frequently in FIV-infected cats ( P <0.001). Renal ultrasonography showed abnormalities in 60/91 FIV-infected cats, with hyperechogenic cortices in 39/91 and enlarged kidneys in 31/91. Conclusions and relevance Hypertension can be excluded as a common cause of renal damage leading to proteinuria in FIV-infected cats. Proteinuria and poorly concentrated urine are common in naturally infected FIV-positive cats, in contrast to azotaemia. Clinicians should cautiously interpret ultrasonographic abnormalities as these occur in over half of FIV-infected cats.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida Felina/fisiopatologia
Hipertensão/veterinária
Nefropatias/veterinária
Proteinúria/veterinária
[Mh] Termos MeSH secundário: Animais
Estudos de Casos e Controles
Gatos
Síndrome de Imunodeficiência Adquirida Felina/complicações
Síndrome de Imunodeficiência Adquirida Felina/diagnóstico por imagem
Síndrome de Imunodeficiência Adquirida Felina/urina
Feminino
Hipertensão/complicações
Vírus da Imunodeficiência Felina
Nefropatias/complicações
Masculino
Proteinúria/complicações
Estudos Retrospectivos
Ultrassonografia/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160617
[St] Status:MEDLINE
[do] DOI:10.1177/1098612X16653165


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[PMID]:27825735
[Au] Autor:Galdo Novo S; Bucafusco D; Diaz LM; Bratanich AC
[Ad] Endereço:Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.
[Ti] Título:Viral diagnostic criteria for Feline immunodeficiency virus and Feline leukemia virus infections in domestic cats from Buenos Aires, Argentina.
[So] Source:Rev Argent Microbiol;48(4):293-297, 2016 Oct - Dec.
[Is] ISSN:0325-7541
[Cp] País de publicação:Argentina
[La] Idioma:eng
[Ab] Resumo:A cross-sectional study was carried out on cats attending the Small Animal Hospital at the Faculty of Veterinary Sciences of the University of Buenos Aires to assess the prevalence and associated risk factors of Feline immunodeficiency virus (FIV) and Feline leukemia virus (FeLV) in the city of Buenos Aires, Argentina. Blood samples from 255 cats with symptoms compatible with FIV or FeLV infection, collected between 2009 and 2013 were analyzed by serology (immunochromatography, IA) and by hemi-nested PCR (n-PCR). The IA and n-PCR assays showed similar percentages of positivity for FIV while the n-PCR test was more sensitive for FeLV. Differences between the diagnostic tests and their choice according to the age of the animal are discussed. The clinical histories of ninety of the 255 cats showed blood profiles similar to others previously reported and revealed a higher risk of infection in male adult cats with outdoor access.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida Felina/diagnóstico
Imunocromatografia/métodos
Vírus da Imunodeficiência Felina/isolamento & purificação
Vírus da Leucemia Felina/isolamento & purificação
Leucemia Felina/diagnóstico
Reação em Cadeia da Polimerase/métodos
Viremia/diagnóstico
[Mh] Termos MeSH secundário: Animais
Argentina/epidemiologia
Gatos/virologia
Estudos Transversais
DNA Viral/análise
Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Síndrome de Imunodeficiência Adquirida Felina/virologia
Feminino
Vírus da Imunodeficiência Felina/genética
Vírus da Imunodeficiência Felina/imunologia
Vírus da Leucemia Felina/genética
Vírus da Leucemia Felina/imunologia
Leucemia Felina/epidemiologia
Leucemia Felina/virologia
Masculino
Prevalência
Provírus/isolamento & purificação
Kit de Reagentes para Diagnóstico
Fatores de Risco
Sensibilidade e Especificidade
Viremia/epidemiologia
Viremia/virologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Reagent Kits, Diagnostic)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170727
[Lr] Data última revisão:
170727
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE


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[PMID]:27808347
[Au] Autor:Hartmann AD; Wilhelm N; Erfle V; Hartmann K
[Ad] Endereço:Dr. Anja Hartmann, KS-Tierarztpraxis, Robert-Koch-Straße 1, 64331 Weiterstadt, E-Mail: info@ks-tierarztpraxis.de.
[Ti] Título:Clinical efficacy of melittin in the treatment of cats infected with the feline immunodeficiency virus.
[Ti] Título:Wirksamkeit von Melittin bei der Behandlung von Katzen mit feliner Immunschwächevirusinfektion..
[So] Source:Tierarztl Prax Ausg K Kleintiere Heimtiere;44(6):417-423, 2016 Dec 05.
[Is] ISSN:1434-1239
[Cp] País de publicação:Germany
[La] Idioma:eng; ger
[Ab] Resumo:OBJECTIVE: The bee venom melittin shows an antiviral efficacy against the human immunodeficiency virus in cell culture. It was shown to be non-toxic for cats. Aim of this pilot study was to investigate the clinical efficacy and side-effects of melittin in cats naturally infected with feline immunodeficiency virus (FIV). MATERIAL AND METHODS: The study was performed as a prospective, placebo-controlled double-blinded trial. Twenty cats were included, of which 10 cats each were treated with either melittin (500 µg/kg body weight) or phosphate-buffered saline (placebo) subcutaneously twice per week. During the treatment period of 6 weeks, the cats' general health status, determined by the Karnofsky's score, and the severity of clinical signs (conjunctivitis and stomatitis) using a clinical scoring system were evaluated. Haematology, biochemistry profiles, lymphocyte subpopulations, CD4/CD8 ratio, and pterines (biopterine, 7-xanthopterine) as surrogate parameters were also compared. RESULTS: The general health status and the clinical scores for conjunctivitis and stomatitis improved in cats treated with melittin. A statistically significant improvement however could only be detected for conjunctivitis in cats treated with melittin compared to cats treated with placebo which was likely due to different scores between both groups at the beginning. No influence on the lymphocyte subpopulations, CD4/CD8 ratio, and pterine concentrations was observed. No side effects occurred in this study. CONCLUSION AND CLINICAL RELEVANCE: In the protocol used in the present study, no significant efficacy of melittin could be detected. However, efficacy of melittin, especially if applied in a higher dosage as in the present study or for a longer period, could be evaluated in further studies. Synergistic effects if used in combination with classic antiretroviral drugs could be an interesting future approach.
[Mh] Termos MeSH primário: Antivirais/uso terapêutico
Síndrome de Imunodeficiência Adquirida Felina/tratamento farmacológico
Vírus da Imunodeficiência Felina/isolamento & purificação
Meliteno/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Relação CD4-CD8
Gatos
Método Duplo-Cego
Subpopulações de Linfócitos
Projetos Piloto
Estudos Prospectivos
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Antiviral Agents); 20449-79-0 (Melitten)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161104
[St] Status:MEDLINE


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[PMID]:27260813
[Au] Autor:Westman ME; Malik R; Hall E; Norris JM
[Ad] Endereço:Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia. Electronic address: mark.westman@sydney.edu.au.
[Ti] Título:Diagnosing feline immunodeficiency virus (FIV) infection in FIV-vaccinated and FIV-unvaccinated cats using saliva.
[So] Source:Comp Immunol Microbiol Infect Dis;46:66-72, 2016 Jun.
[Is] ISSN:1878-1667
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We recently showed that two immunochromatography point-of-care FIV antibody test kits (Witness FeLV/FIV and Anigen Rapid FIV/FeLV) were able to correctly assign FIV infection status, irrespective of FIV vaccination history, using whole blood as the diagnostic specimen. A third FIV antibody test kit, SNAP FIV/FeLV Combo (an enzyme-linked immunosorbent assay [ELISA]), was unable to differentiate antibodies produced in response to FIV vaccination from those incited by FIV infection. The aim of this study was to determine if saliva is a suitable diagnostic specimen using the same well characterized feline cohort. FIV infection status of these cats had been determined previously using a combination of serology, polymerase chain reaction (PCR) testing and virus isolation. This final assignment was then compared to results obtained using saliva as the diagnostic specimen utilizing the same three point-of-care FIV antibody test kits and commercially available PCR assay (FIV RealPCR). In a population of cats where one third (117/356; 33%) were FIV-vaccinated, both immunochromatography test kits accurately diagnosed FIV infection using saliva via a centrifugation method, irrespective of FIV vaccination history. For FIV diagnosis using saliva, the specificity of Anigen Rapid FIV/FeLV and Witness FeLV/FIV was 100%, while the sensitivity of these kits was 96% and 92% respectively. SNAP FIV/FeLV Combo respectively. SNAP FIV/FeLV Combo had a specificity of 98% and sensitivity of 44%, while FIV RealPCR testing had a specificity of 100% and sensitivity of 72% using saliva. A revised direct method of saliva testing was trialed on a subset of FIV-infected cats (n=14), resulting in 14, 7 and 0 FIV positive results using Anigen Rapid FIV/FeLV, Witness FeLV/FIV and SNAP FIV/FeLV Combo, respectively. These results demonstrate that saliva can be used to diagnose FIV infection, irrespective of FIV vaccination history, using either a centrifugation method (Anigen Rapid FIV/FeLV and Witness FeLV/FIV) or a direct method (Anigen Rapid FIV/FeLV). Collection of a saliva specimen therefore provides an acceptable alternative to venipuncture (i) in fractious cats where saliva may be easier to obtain than whole blood, (ii) in settings when a veterinarian or trained technician is unavailable to collect blood and (iii) in shelters where FIV testing is undertaken prior to adoption but additional blood testing is not required.
[Mh] Termos MeSH primário: Anticorpos Antivirais/análise
Síndrome de Imunodeficiência Adquirida Felina/diagnóstico
Vírus da Imunodeficiência Felina/imunologia
Vírus da Imunodeficiência Felina/isolamento & purificação
Saliva/imunologia
Saliva/virologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Gatos
Ensaio de Imunoadsorção Enzimática
Síndrome de Imunodeficiência Adquirida Felina/imunologia
Síndrome de Imunodeficiência Adquirida Felina/virologia
Feminino
Vírus da Imunodeficiência Felina/genética
Masculino
Sistemas Automatizados de Assistência Junto ao Leito/economia
Reação em Cadeia da Polimerase
Kit de Reagentes para Diagnóstico
Reação em Cadeia da Polimerase em Tempo Real
Sensibilidade e Especificidade
Vacinas Virais/administração & dosagem
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral); 0 (Reagent Kits, Diagnostic); 0 (Viral Vaccines)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160605
[St] Status:MEDLINE



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