Base de dados : MEDLINE
Pesquisa : C02.782.815.622 [Categoria DeCS]
Referências encontradas : 244 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 25 ir para página                         

  1 / 244 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28918382
[Au] Autor:Stavisky J; Dean RS; Molloy MH
[Ad] Endereço:Centre for Evidence-based Veterinary Medicine, School of Veterinary Medicine and Science, The University of Nottingham, Loughborough, UK.
[Ti] Título:Prevalence of and risk factors for FIV and FeLV infection in two shelters in the United Kingdom (2011-2012).
[So] Source:Vet Rec;181(17):451, 2017 Oct 28.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aims of this study were to determine the prevalence of feline leukaemia virus (FeLV) and feline immunodeficiency virus (FIV) infections in cats presented to two RSPCA (Royal Society for the Prevention of Cruelty to Animals) animal rehoming centres and to identify risk factors for infection. All cats presented at each centre between August 2011 and August 2012 were subjected to a patient-side test for FeLV/FIV on entry. Kittens under three months and cats euthanased within a short time of presentation were excluded from the study. Univariable and multivariable logistic regression were used to separately determine risk factors for FeLV and FIV infections. At shelter A, the prevalence of FIV infection was 11.4 per cent (54/474) and FeLV infection was 3 per cent (14/473), with two FIV/FeLV coinfections identified. At shelter B, the prevalence of FIV infection was 3 per cent (4/135) and FeLV infection was 0 per cent (0/135). Cats at shelter A were significantly more likely than those at shelter B to test positive for FIV (p=0.0024) and FeLV (p=0.048). Male cats were more likely to be infected with FIV (odds ratio 27.1, p=0.001), and thin body condition and musculoskeletal disease were associated with risk of FeLV. Overall, FIV-positive and FeLV-positive cats were significantly older (median ages 5.1 and 4.75 years, respectively) than the uninfected populations (median ages 3.4 and 3.5 years, respectively). This study shows that the prevalence of these diseases varies between shelter populations. Local knowledge combined with the risk factors identified may be useful in focusing resources for population testing strategies.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Abrigo para Animais/estatística & dados numéricos
Vírus da Imunodeficiência Felina/isolamento & purificação
Vírus da Leucemia Felina/isolamento & purificação
Leucemia Felina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Gatos
Instituições de Caridade
Feminino
Masculino
Prevalência
Fatores de Risco
Reino Unido/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170918
[St] Status:MEDLINE
[do] DOI:10.1136/vr.103857


  2 / 244 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28780918
[Au] Autor:Sato S; Kabeya H; Negishi A; Tsujimoto H; Nishigaki K; Endo Y; Maruyama S
[Ad] Endereço:Laboratory of Veterinary Public Health, Department of Veterinary Medicine,College of Bioresource Sciences, Nihon University,1866 Kameino, Fujisawa, Kanagawa Prefecture, 252-0880,Japan.
[Ti] Título:Molecular survey of Bartonella henselae and Bartonella clarridgeiae in pet cats across Japan by species-specific nested-PCR.
[So] Source:Epidemiol Infect;145(13):2694-2700, 2017 10.
[Is] ISSN:1469-4409
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cats are known to be the main reservoir for Bartonella henselae and Bartonella clarridgeiae, which are the agents of 'cat-scratch disease' in humans. In the present study, we investigated the prevalence of the two Bartonella species on 1754 cat bloods collected from all prefectures in Japan during 2007-2008 by a nested-polymerase chain reaction (PCR) targeting the 16S-23S rRNA internal transcribed spacer region. Overall, Bartonella DNA was detected in 4·6% (80/1754) of the cats examined. The nested-PCR showed that 48·8% (39/80) of the positive cats were infected with B. henselae mono-infection, 33·8% (27/80) with B. clarridgeiae mono-infection and 17·5% (14/80) were infected with both species. The prevalence (5·9%; 65/1103) of Bartonella infection in the western part of Japan was significantly higher than that (2·3%; 15/651) of eastern Japan (P < 0·001). Statistical analysis of the cats examined suggested a significant association between Bartonella infection and FeLV infection (OR = 1·9; 95% CI = 1·1-3·4), but not with FIV infection (OR = 1·6; 95% CI = 1·0-2·6).
[Mh] Termos MeSH primário: Bartonella/isolamento & purificação
Doença da Arranhadura de Gato/veterinária
Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Leucemia Felina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Bartonella/classificação
Bartonella/genética
Bartonella henselae/classificação
Bartonella henselae/genética
Bartonella henselae/isolamento & purificação
Doença da Arranhadura de Gato/epidemiologia
Doença da Arranhadura de Gato/microbiologia
Gatos
Síndrome de Imunodeficiência Adquirida Felina/virologia
Feminino
Vírus da Imunodeficiência Felina/isolamento & purificação
Japão/epidemiologia
Vírus da Leucemia Felina/isolamento & purificação
Leucemia Felina/virologia
Masculino
Reação em Cadeia da Polimerase/veterinária
Prevalência
RNA Ribossômico/análise
RNA Viral/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal); 0 (RNA, Viral)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE
[do] DOI:10.1017/S0950268817001601


  3 / 244 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28768854
[Au] Autor:Kawasaki J; Kawamura M; Ohsato Y; Ito J; Nishigaki K
[Ad] Endereço:Laboratory of Molecular Immunology and Infectious Disease, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, Japan.
[Ti] Título:Presence of a Shared 5'-Leader Sequence in Ancestral Human and Mammalian Retroviruses and Its Transduction into Feline Leukemia Virus.
[So] Source:J Virol;91(20), 2017 Oct 15.
[Is] ISSN:1098-5514
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recombination events induce significant genetic changes, and this process can result in virus genetic diversity or in the generation of novel pathogenicity. We discovered a new recombinant feline leukemia virus (FeLV) gene harboring an unrelated insertion, termed the X region, which was derived from endogenous gammaretrovirus 4 (FcERV-gamma4). The identified FcERV-gamma4 proviruses have lost their coding capabilities, but some can express their viral RNA in feline tissues. Although the X-region-carrying recombinant FeLVs appeared to be replication-defective viruses, they were detected in 6.4% of tested FeLV-infected cats. All isolated recombinant FeLV clones commonly incorporated a middle part of the FcERV-gamma4 5'-leader region as an X region. Surprisingly, a sequence corresponding to the portion contained in all X regions is also present in at least 13 endogenous retroviruses (ERVs) observed in the cat, human, primate, and pig genomes. We termed this shared genetic feature the commonly shared (CS) sequence. Despite our phylogenetic analysis indicating that all CS-sequence-carrying ERVs are classified as gammaretroviruses, no obvious closeness was revealed among these ERVs. However, the Shannon entropy in the CS sequence was lower than that in other parts of the provirus genome. Notably, the CS sequence of human endogenous retrovirus T had 73.8% similarity with that of FcERV-gamma4, and specific signals were detected in the human genome by Southern blot analysis using a probe for the FcERV-gamma4 CS sequence. Our results provide an interesting evolutionary history for CS-sequence circulation among several distinct ancestral viruses and a novel recombined virus over a prolonged period. Recombination among ERVs or modern viral genomes causes a rapid evolution of retroviruses, and this phenomenon can result in the serious situation of viral disease reemergence. We identified a novel recombinant FeLV gene that contains an unrelated sequence, termed the X region. This region originated from the 5' leader of FcERV-gamma4, a replication-incompetent feline ERV. Surprisingly, a sequence corresponding to the X region is also present in the 5' portion of other ERVs, including human endogenous retroviruses. Scattered copies of the ERVs carrying the unique genetic feature, here named the commonly shared (CS) sequence, were found in each host genome, suggesting that ancestral viruses may have captured and maintained the CS sequence. More recently, a novel recombinant FeLV hijacked the CS sequence from inactivated FcERV-gamma4 as the X region. Therefore, tracing the CS sequences can provide unique models for not only the modern reservoir of new recombinant viruses but also the genetic features shared among ancient retroviruses.
[Mh] Termos MeSH primário: Regiões 5´ não Traduzidas/genética
Retrovirus Endógenos/genética
Genes gag
Genoma Viral
Vírus da Leucemia Felina/genética
Recombinação Genética
[Mh] Termos MeSH secundário: Animais
Gatos/virologia
Evolução Molecular
Gammaretrovirus/genética
Seres Humanos/virologia
Leucemia Felina/virologia
Mamíferos/genética
Mamíferos/virologia
Filogenia
Provírus/genética
Provírus/fisiologia
RNA Viral/genética
Suínos/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (5' Untranslated Regions); 0 (RNA, Viral)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE


  4 / 244 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28510253
[Au] Autor:Lacerda LC; Silva AN; Freitas JS; Cruz RDS; Said RA; Munhoz AD
[Ad] Endereço:Departamento de Ciências Agrárias e Ambientais, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
[Ti] Título:Feline immunodeficiency virus and feline leukemia virus: frequency and associated factors in cats in northeastern Brazil.
[So] Source:Genet Mol Res;16(2), 2017 May 10.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Our aims were to determine the frequencies of feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) in owned and stray cats in the northeastern region of Brazil, ascertain the status of FeLV infection, and investigate potential associated factors among the owned cats. Blood samples from 200 asymptomatic owned cats and 30 stray cats were processed using nested PCR and commercial immunochromatographic tests to diagnose infections. To evaluate the factors associated with FIV and/or FeLV in owned cats, a semi-structured interview was conducted with each owner about the animal's environment, and these data were subjected to unconditional logistic regression. The frequencies for owned cats were 6% (12/200) and 3% (6/200) for FIV and FeLV, respectively. No owned cat was positive for both viruses. Stray cats showed frequencies of 6.66% (2/30) and 0% (0/30) for FIV and FeLV, respectively. Contact with other cats and living in peri-urban areas were considered to be risk factors (P < 0.05) for FIV. We did not identify any factors associated with infections with FeLV. Our results confirm the presence of these two retroviruses in the region under study. Our use of different diagnostic techniques allowed us to determine the frequency of retroviruses in the feline population more accurately, particularly with regard to infections by FeLV, which have complex pathogenesis.
[Mh] Termos MeSH primário: Gatos/virologia
Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Vírus da Imunodeficiência Felina/genética
Vírus da Leucemia Felina/genética
Leucemia Felina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Brasil
Síndrome de Imunodeficiência Adquirida Felina/virologia
Feminino
Leucemia Felina/virologia
Masculino
Animais de Estimação/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.4238/gmr16029633


  5 / 244 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27825735
[Au] Autor:Galdo Novo S; Bucafusco D; Diaz LM; Bratanich AC
[Ad] Endereço:Cátedra de Virología, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.
[Ti] Título:Viral diagnostic criteria for Feline immunodeficiency virus and Feline leukemia virus infections in domestic cats from Buenos Aires, Argentina.
[So] Source:Rev Argent Microbiol;48(4):293-297, 2016 Oct - Dec.
[Is] ISSN:0325-7541
[Cp] País de publicação:Argentina
[La] Idioma:eng
[Ab] Resumo:A cross-sectional study was carried out on cats attending the Small Animal Hospital at the Faculty of Veterinary Sciences of the University of Buenos Aires to assess the prevalence and associated risk factors of Feline immunodeficiency virus (FIV) and Feline leukemia virus (FeLV) in the city of Buenos Aires, Argentina. Blood samples from 255 cats with symptoms compatible with FIV or FeLV infection, collected between 2009 and 2013 were analyzed by serology (immunochromatography, IA) and by hemi-nested PCR (n-PCR). The IA and n-PCR assays showed similar percentages of positivity for FIV while the n-PCR test was more sensitive for FeLV. Differences between the diagnostic tests and their choice according to the age of the animal are discussed. The clinical histories of ninety of the 255 cats showed blood profiles similar to others previously reported and revealed a higher risk of infection in male adult cats with outdoor access.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida Felina/diagnóstico
Imunocromatografia/métodos
Vírus da Imunodeficiência Felina/isolamento & purificação
Vírus da Leucemia Felina/isolamento & purificação
Leucemia Felina/diagnóstico
Reação em Cadeia da Polimerase/métodos
Viremia/diagnóstico
[Mh] Termos MeSH secundário: Animais
Argentina/epidemiologia
Gatos/virologia
Estudos Transversais
DNA Viral/análise
Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Síndrome de Imunodeficiência Adquirida Felina/virologia
Feminino
Vírus da Imunodeficiência Felina/genética
Vírus da Imunodeficiência Felina/imunologia
Vírus da Leucemia Felina/genética
Vírus da Leucemia Felina/imunologia
Leucemia Felina/epidemiologia
Leucemia Felina/virologia
Masculino
Prevalência
Provírus/isolamento & purificação
Kit de Reagentes para Diagnóstico
Fatores de Risco
Sensibilidade e Especificidade
Viremia/epidemiologia
Viremia/virologia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Viral); 0 (Reagent Kits, Diagnostic)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170727
[Lr] Data última revisão:
170727
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161110
[St] Status:MEDLINE


  6 / 244 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:26744011
[Au] Autor:Garigliany M; Jolly S; Dive M; Bayrou C; Berthemin S; Robin P; Godenir R; Petry J; Dahout S; Cassart D; Thiry E; Desmecht D; Saegerman C
[Ad] Endereço:Department of Morphology and Pathology, Fundamental and Applied Research for Animals and Health (FARAH) Center, Faculty of Veterinary Medicine, University of Liège, Avenue de Cureghem 6 (B43), Liège 4000, Belgium.
[Ti] Título:Risk factors and effect of selective removal on retroviral infections prevalence in Belgian stray cats.
[So] Source:Vet Rec;178(2):45, 2016 Jan 09.
[Is] ISSN:2042-7670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate the effect of several risk/protective factors and predictors on the prevalence of feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) infections in 302 stray cats captured during a trap-neuter-release programme in a mixed urban-rural area from Belgium, from 2010 to 2012. The impact of selective removal of FIV-positive cats on the apparent prevalence in the remaining population over this three-year period was also assessed. The seroprevalences over three years were 18.8 per cent for FIV and 0.7 per cent for FeLV. For FIV, the seroprevalence decreased significantly from the first year of the programme (2010; 30.5 per cent) to the last (2012; 13.1 per cent). Sex (male) and age (adult and old cats) were risk factors, while the year of sampling (years 2011 and 2012) was a protective factor. Age, sex and location were the most relevant predictors of FIV status. The data presented in this study revealed a very high FIV seroprevalence in Belgian stray cats, while FeLV was almost absent. The selective removal of positive cats had a drastic effect on the FIV seroprevalence in the remaining cat population.
[Mh] Termos MeSH primário: Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Leucemia Felina/epidemiologia
Propriedade/estatística & dados numéricos
Controle da População/métodos
[Mh] Termos MeSH secundário: Animais
Bélgica/epidemiologia
Gatos
Prevalência
Fatores de Risco
Estudos Soroepidemiológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160108
[Lr] Data última revisão:
160108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160109
[St] Status:MEDLINE
[do] DOI:10.1136/vr.103314


  7 / 244 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
Texto completo
[PMID]:26689419
[Au] Autor:Nesina S; Katrin Helfer-Hungerbuehler A; Riond B; Boretti FS; Willi B; Meli ML; Grest P; Hofmann-Lehmann R
[Ad] Endereço:Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland. s.nesina@gmx.ch.
[Ti] Título:Retroviral DNA--the silent winner: blood transfusion containing latent feline leukemia provirus causes infection and disease in naïve recipient cats.
[So] Source:Retrovirology;12:105, 2015 Dec 21.
[Is] ISSN:1742-4690
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The feline leukemia virus (FeLV) is a gamma-retrovirus of domestic cats that was discovered half a century ago. Cats that are infected with FeLV may develop a progressive infection resulting in persistent viremia, immunodeficiency, tumors, anemia and death. A significant number of cats mount a protective immune response that suppresses viremia; these cats develop a regressive infection characterized by the absence of viral replication and the presence of low levels of proviral DNA. The biological importance of these latter provirus carriers is largely unknown. RESULTS: Here, we demonstrate that ten cats that received a transfusion of blood from aviremic provirus carriers developed active FeLV infections, some with a progressive outcome and the development of fatal FeLV-associated disease. The infection outcome, disease spectrum and evolution into FeLV-C in one cat mirrored those of natural infection. Two cats developed persistent antigenemia; six cats were transiently antigenemic. Reactivation of infection occurred in some cats. One recipient developed non-regenerative anemia associated with FeLV-C, and four others developed a T-cell lymphoma, one with secondary lymphoblastic leukemia. Five of the ten recipient cats received provirus-positive aviremic blood, whereas the other five received provirus- and viral RNA-positive but aviremic blood. Notably, the cats that received blood containing only proviral DNA exhibited a later onset but graver outcome of FeLV infection than the cats that were transfused with blood containing proviral DNA and viral RNA. Leukocyte counts and cytokine analyses indicated that the immune system of the latter cats reacted quicker and more efficiently. CONCLUSIONS: Our results underline the biological and epidemiological relevance of FeLV provirus carriers and the risk of inadvertent FeLV transmission via blood transfusion and demonstrate the replication capacity of proviral DNA if uncontrolled by the immune system. Our results have implications not only for veterinary medicine, such as the requirement for testing blood donors and blood products for FeLV provirus by sensitive polymerase chain reaction, but are also of general interest by revealing the importance of latent retroviral DNA in infected hosts. When aiming to eliminate a retroviral infection from a population, provirus carriers must be considered.
[Mh] Termos MeSH primário: Transfusão de Sangue/veterinária
DNA Viral
Leucemia Felina/transmissão
Provírus/genética
Infecções Tumorais por Vírus/veterinária
Latência Viral
[Mh] Termos MeSH secundário: Anemia/veterinária
Anemia/virologia
Animais
Gatos
Vírus da Leucemia Felina/imunologia
Vírus da Leucemia Felina/fisiologia
Leucemia Felina/imunologia
Leucemia Felina/mortalidade
Leucemia Felina/virologia
Linfoma de Células T/veterinária
Linfoma de Células T/virologia
Reação em Cadeia da Polimerase
Leucemia-Linfoma Linfoblástico de Células Precursoras/veterinária
Leucemia-Linfoma Linfoblástico de Células Precursoras/virologia
Provírus/imunologia
Infecções Tumorais por Vírus/imunologia
Infecções Tumorais por Vírus/transmissão
Infecções Tumorais por Vírus/virologia
Carga Viral
Latência Viral/imunologia
Replicação Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Viral)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151223
[St] Status:MEDLINE
[do] DOI:10.1186/s12977-015-0231-z


  8 / 244 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:26054227
[Au] Autor:Lehmann M; Weber K; Rauch G; Hofmann-Lehmann R; Hosie MJ; Meli ML; Hartmann K
[Ti] Título:[Efficacy of siRNA on feline leukemia virus replication in vitro].
[Ti] Título:Wirksamkeit von siRNA auf die Vermehrung des felinen Leukämievirus in vitro..
[So] Source:Berl Munch Tierarztl Wochenschr;128(5-6):209-17, 2015 May-Jun.
[Is] ISSN:0005-9366
[Cp] País de publicação:Germany
[La] Idioma:ger
[Ab] Resumo:Feline leukemia virus (FeLV) can lead to severe clinical signs in cats. Until now, there is no effective therapy for FeLV-infected cats. RNA interference-based antiviral therapy is a new concept. Specific small interfering RNA (siRNA) are designed complementary to the mRNA of a target region, and thus inhibit replication. Several studies have proven efficacy of siRNAs in inhibiting virus replication. The aim of this study was to evaluate the inhibitory potential of siRNAs against FeLV replication in vitro. siRNAs against the FeLV env gene and the host cell surface receptor (feTHTR1) which is used by FeLV-A for entry as well as siRNA that were not complementary to the FeLV or cat genome, were tested. Crandell feline kidney cells (CrFK cells) were transfected with FeLV-A/Glasgow-1. On day 13, infected cells were transfected with siRNAs. As control, cells were mock-transfected or treated with azidothymidine (AZT) (5 µg/ml). Culture supernatants were analyzed for FeLV RNA using quantitative real-time RT-PCR and for FeLV p27 by ELISA every 24 hours for five days. All siRNAs significantly reduced viral RNA and p27 production, starting after 48 hours. The fact that non-complementary siRNAs also inhibited virus replication may lead to the conclusion that unspecific mechanisms rather than specific binding lead to inhibition.
[Mh] Termos MeSH primário: Vírus da Leucemia Felina/fisiologia
RNA Interferente Pequeno/farmacologia
Replicação Viral/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Gatos
Linhagem Celular
Vírus da Leucemia Felina/genética
Leucemia Felina/terapia
Leucemia Felina/virologia
RNA Viral/análise
RNA Viral/genética
Reação em Cadeia da Polimerase em Tempo Real
Replicação Viral/genética
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Small Interfering); 0 (RNA, Viral)
[Em] Mês de entrada:1508
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150610
[St] Status:MEDLINE


  9 / 244 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25687627
[Au] Autor:Tekelioglu BK; Berriatua E; Turan N; Helps CR; Kocak M; Yilmaz H
[Ad] Endereço:Macka Veterinary Clinic, Besiktas, Istanbul, Turkey.
[Ti] Título:A retrospective clinical and epidemiological study on feline coronavirus (FCoV) in cats in Istanbul, Turkey.
[So] Source:Prev Vet Med;119(1-2):41-7, 2015 Apr 01.
[Is] ISSN:1873-1716
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The presence of antibodies to feline coronavirus (FCoV) and feline immunodeficiency virus (FIV), together with feline leukemia virus (FeLV) antigen was investigated in 169 ill household and stray cats attending a veterinary surgery in Istanbul in 2009-14. The estimated FCoV and FIV seroprevalence (95% confidence intervals) were 37% (30-45%) and 11% (6-16%), respectively and FeLV prevalence was 1% (0-3%). FCoV seroprevalence increased until 2 years of age, was highest in 2014 and among household cats living with other cats and with outdoor access, and was lower in FIV seropositive compared to seronegative cats. Symptoms typically associated with wet feline infectious peritonitis (FIP) including ascites, abdominal distention or pleural effusion, coupled in many cases with non-antibiotic responsive fever, were observed in 19% (32/169) of cats, and 75% (24/32) of these cats were FCoV seropositive. FCoV seropositivity was also associated with a high white blood cell count, high plasma globulin, low plasma albumin and low blood urea nitrogen. The percentage of FCoV seropositive and seronegative cats that died in spite of supportive veterinary treatment was 33% (21/63) and 12% (13/106), respectively. These results indicate that FCoV is widespread and has a severe clinical impact in cats from Istanbul. Moreover, the incidence of FCoV infections could be rising, and in the absence of effective vaccination cat owners need to be made aware of ways to minimize the spread of this virus.
[Mh] Termos MeSH primário: Doenças do Gato/epidemiologia
Infecções por Coronavirus/veterinária
Coronavirus Felino/isolamento & purificação
Peritonite Infecciosa Felina/epidemiologia
[Mh] Termos MeSH secundário: Animais
Anticorpos Antivirais/sangue
Doenças do Gato/virologia
Gatos
Infecções por Coronavirus/epidemiologia
Infecções por Coronavirus/virologia
Síndrome de Imunodeficiência Adquirida Felina/epidemiologia
Síndrome de Imunodeficiência Adquirida Felina/virologia
Peritonite Infecciosa Felina/virologia
Feminino
Vírus da Imunodeficiência Felina/isolamento & purificação
Vírus da Leucemia Felina/isolamento & purificação
Leucemia Felina/epidemiologia
Leucemia Felina/virologia
Masculino
Prevalência
Estudos Retrospectivos
Estudos Soroepidemiológicos
Turquia/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Viral)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150316
[Lr] Data última revisão:
150316
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150218
[St] Status:MEDLINE


  10 / 244 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:25681660
[Au] Autor:Stuke K; King V; Southwick K; Stoeva MI; Thomas A; Winkler MT
[Ti] Título:In response to letter to the editor (Regina Hofmann-Lehmann, Laura S. Levy and Brian Willett)--Comparing the efficacy of FeLV vaccines.
[So] Source:Vaccine;33(24):2739-40, 2015 Jun 04.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Mh] Termos MeSH primário: Antígenos Virais/sangue
Produtos do Gene gag/sangue
Leucemia Felina/prevenção & controle
Proteínas Oncogênicas de Retroviridae/administração & dosagem
Vacinação/veterinária
Vacinas Virais/administração & dosagem
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:COMMENT; LETTER
[Nm] Nome de substância:
0 (Antigens, Viral); 0 (Gene Products, gag); 0 (Retroviridae Proteins, Oncogenic); 0 (Viral Vaccines)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:150518
[Lr] Data última revisão:
150518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150215
[St] Status:MEDLINE



página 1 de 25 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde