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[PMID]:29318257
[Au] Autor:Abbasi J
[Ti] Título:iPhone-Based Device Helps Treat River Blindness.
[So] Source:JAMA;319(2):113, 2018 Jan 09.
[Is] ISSN:1538-3598
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Telefone Celular
Loa/isolamento & purificação
Loíase/diagnóstico
Microscopia de Vídeo
Oncocercose Ocular
[Mh] Termos MeSH secundário: Animais
Seres Humanos
[Pt] Tipo de publicação:NEWS
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180123
[Lr] Data última revisão:
180123
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1001/jama.2017.20077


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[PMID]:28209852
[Au] Autor:Vogel G
[Ti] Título:Parasitic worm may trigger mystery nodding syndrome.
[So] Source:Science;355(6326):678, 2017 Feb 17.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Autoantígenos/imunologia
Autoimunidade
Proteínas do Citoesqueleto/imunologia
Doenças Negligenciadas/complicações
Síndrome do Cabeceio/imunologia
Síndrome do Cabeceio/parasitologia
Oncocercose Ocular/complicações
[Mh] Termos MeSH secundário: Animais
Autoanticorpos/sangue
Criança
Pré-Escolar
Controle de Doenças Transmissíveis
Proteínas de Helminto/imunologia
Seres Humanos
Ivermectina/administração & dosagem
Doenças Negligenciadas/parasitologia
Doenças Negligenciadas/prevenção & controle
Síndrome do Cabeceio/sangue
Onchocerca volvulus/imunologia
Oncocercose Ocular/parasitologia
Oncocercose Ocular/prevenção & controle
Sudão do Sul/epidemiologia
Uganda/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Autoantibodies); 0 (Autoantigens); 0 (Cytoskeletal Proteins); 0 (Helminth Proteins); 0 (LMOD1 protein, human); 70288-86-7 (Ivermectin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE
[do] DOI:10.1126/science.355.6326.678


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[PMID]:28111713
[Au] Autor:Lagatie O; Batsa Debrah L; Debrah A; Stuyver LJ
[Ad] Endereço:Janssen Diagnostics, Janssen R&D, Turnhoutseweg 30, 2340, Beerse, Belgium. olagatie@its.jnj.com.
[Ti] Título:Plasma-derived parasitic microRNAs have insufficient concentrations to be used as diagnostic biomarker for detection of Onchocerca volvulus infection or treatment monitoring using LNA-based RT-qPCR.
[So] Source:Parasitol Res;116(3):1013-1022, 2017 Mar.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:River blindness, caused by infection with the filarial nematode Onchocerca volvulus, is a neglected tropical disease affecting millions of people. There is a clear need for diagnostic tools capable of identifying infected patients, but that can also be used for monitoring disease progression and treatment efficacy. Plasma-derived parasitic microRNAs have been suggested as potential candidates for such diagnostic tools. We have investigated whether these parasitic microRNAs are present in sufficient quantity in plasma of Onchocerca-infected patients to be used as a diagnostic biomarker for detection of O. volvulus infection or treatment monitoring. Plasma samples were collected from different sources (23 nodule-positive individuals and 20 microfilaridermic individuals), microRNAs (miRNAs) were extracted using Qiagen miRNeasy kit, and a set of 17 parasitic miRNAs was evaluated on these miRNA extracts using miRCURY Locked Nucleic Acid (LNA) Universal RT microRNA PCR system. Of the 17 miRNAs evaluated, only 7 miRNAs were found to show detectable signal in a number of samples: bma-miR-236-1, bma-miR-71, ov-miR71-22nt, ov-miR-71-23nt, ov-miR-100d, ov-bantam-a, and ov-miR-87-3p. Subsequent melting curve analysis, however, indicated that the signals observed for ov-miR-71 variants and ov-miR-87-3p are non-specific. The other miRNAs only showed positive signal in one or few samples with Cq values just below the cutoff. Our data indicate that parasitic miRNAs are not present in circulation at a sufficiently high level to be used as biomarker for O. volvulus infection or treatment monitoring using LNA-based RT-qPCR analysis.
[Mh] Termos MeSH primário: DNA de Helmintos/genética
MicroRNAs/genética
Oligonucleotídeos/genética
Onchocerca volvulus/isolamento & purificação
Oncocercose Ocular/parasitologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Animais
Anti-Helmínticos/uso terapêutico
Biomarcadores/sangue
DNA de Helmintos/sangue
DNA de Helmintos/metabolismo
Feminino
Seres Humanos
Masculino
MicroRNAs/sangue
MicroRNAs/metabolismo
Meia-Idade
Oligonucleotídeos/metabolismo
Onchocerca volvulus/genética
Onchocerca volvulus/metabolismo
Oncocercose Ocular/sangue
Oncocercose Ocular/diagnóstico
Oncocercose Ocular/tratamento farmacológico
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Biomarkers); 0 (DNA, Helminth); 0 (MicroRNAs); 0 (Oligonucleotides); 0 (locked nucleic acid)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-017-5382-5


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[PMID]:27801556
[Ti] Título:Progress towards eliminating onchocerciasis in the WHO Region of the Americas: verification of elimination of transmission in Guatemala.
[So] Source:Wkly Epidemiol Rec;91(43):501-5, 2016 Oct 28.
[Is] ISSN:0049-8114
[Cp] País de publicação:Switzerland
[La] Idioma:eng; fre
[Mh] Termos MeSH primário: Erradicação de Doenças
Onchocerca volvulus
Oncocercose/prevenção & controle
[Mh] Termos MeSH secundário: Américas/epidemiologia
Animais
Feminino
Filaricidas/uso terapêutico
Guatemala/epidemiologia
Seres Humanos
Ivermectina/uso terapêutico
Masculino
Oncocercose/epidemiologia
Oncocercose/transmissão
Oncocercose Ocular/prevenção & controle
Avaliação de Programas e Projetos de Saúde
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Filaricides); 70288-86-7 (Ivermectin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161102
[St] Status:MEDLINE


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[PMID]:27756456
[Au] Autor:Basáñez MG; Walker M; Turner HC; Coffeng LE; de Vlas SJ; Stolk WA
[Ad] Endereço:Imperial College London, London, United Kingdom.
[Ti] Título:River Blindness: Mathematical Models for Control and Elimination.
[So] Source:Adv Parasitol;94:247-341, 2016.
[Is] ISSN:2163-6079
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Human onchocerciasis (river blindness) is one of the few neglected tropical diseases (NTDs) whose control strategies have been informed by mathematical modelling. With the change in focus from elimination of the disease burden to elimination of Onchocerca volvulus, much remains to be done to refine, calibrate and validate existing models. Under the impetus of the NTD Modelling Consortium, the teams that developed EPIONCHO and ONCHOSIM have joined forces to compare and improve these frameworks to better assist ongoing elimination efforts. We review their current versions and describe how they are being used to address two key questions: (1) where can onchocerciasis be eliminated with current intervention strategies by 2020/2025? and (2) what alternative/complementary strategies could help to accelerate elimination where (1) cannot be achieved? The control and elimination of onchocerciasis from the African continent is at a crucial crossroad. The African Programme for Onchocerciasis Control closed at the end of 2015, and although a new platform for support and integration of NTD control has been launched, the disease will have to compete with a myriad of other national health priorities at a pivotal time in the road to elimination. However, never before had onchocerciasis control a better arsenal of intervention strategies as well as diagnostics. It is, therefore, timely to present two models of different geneses and modelling traditions as they come together to produce robust decision-support tools. We start by describing the structural and parametric assumptions of EPIONCHO and ONCHOSIM; we continue by summarizing the modelling of current treatment strategies with annual (or biannual) mass ivermectin distribution and introduce a number of alternative strategies, including other microfilaricidal therapies (such as moxidectin), macrofilaricidal (anti-wolbachial) treatments, focal vector control and the possibility of an onchocerciasis vaccine. We conclude by discussing challenges, opportunities and future directions.
[Mh] Termos MeSH primário: Antiparasitários/administração & dosagem
Modelos Teóricos
Onchocerca volvulus/efeitos dos fármacos
Oncocercose Ocular/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Erradicação de Doenças
Seres Humanos
Ivermectina/administração & dosagem
Macrolídeos/administração & dosagem
Microfilárias
Onchocerca volvulus/imunologia
Onchocerca volvulus/fisiologia
Oncocercose Ocular/tratamento farmacológico
Oncocercose Ocular/parasitologia
Vacinas
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Nome de substância:
0 (Antiparasitic Agents); 0 (Macrolides); 0 (Vaccines); 51570-36-6 (milbemycin); 70288-86-7 (Ivermectin)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170310
[Lr] Data última revisão:
170310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE


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[PMID]:27645887
[Au] Autor:Hernández-González A; Moya L; Perteguer MJ; Herrador Z; Nguema R; Nguema J; Aparicio P; Benito A; Gárate T
[Ad] Endereço:Helminth Unit, Parasitology Department, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Crtra. Majadahonda-Pozuelo, km 2.2, 28220, Majadahonda, Madrid, Spain.
[Ti] Título:Evaluation of onchocerciasis seroprevalence in Bioko Island (Equatorial Guinea) after years of disease control programmes.
[So] Source:Parasit Vectors;9(1):509, 2016 Sep 20.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Onchocerciasis or "river blindness" is a chronic parasitic disease caused by the filarial worm Onchocerca volvulus, transmitted through infected blackflies (Simulium spp.). Bioko Island (Equatorial Guinea) used to show a high endemicity for onchocerciasis. During the last years, the disease control programmes using different larvicides and ivermectin administration have considerably reduced the prevalence and intensity of infection. Based on this new epidemiological scenario, in the present work we aimed to assess the impact of the strategies applied against onchocerciasis in Bioko Island by an evaluation of IgG4 antibodies specific for recombinant Ov-16 in ELISA. METHODS: A cross-sectional study was conducted in Bioko Island from mid-January to mid-February, 2014. Twenty communities were randomly selected from rural and urban settings. A total of 140 households were chosen. In every selected household, all individuals aged 5 years and above were recruited; 544 study participants agreed to be part of this work. No previous data on onchocerciasis seroprevalence in the selected communities were available. Blood samples were collected and used in an "ELISA in-house" prepared with recombinant Ov-16, expressed and further purified. IgG4 antibodies specific for recombinant Ov-16 were evaluated by ELISA in all of the participants. RESULTS: Based on the Ov-16 ELISA, the onchocerciasis seroprevalence was 7.9 %, mainly concentrated in rural settings; samples from community Catedral Ela Nguema (# 16) were missed during the field work. Among the rural setups, communities Inasa Maule (# 7), Ruiché (# 20) and Barrios Adyacentes Riaba (# 14), had the highest seropositivity percentages (29.2, 26.9 and 23.8 %, respectively). With respect to the urban settings, we did not find any positive case in communities Manzana Casa Bola (# 3), Colas Sesgas (# 6), Getesa (# 8), Moka Bioko (# 9), Impecsa (# 10), Baney Zona Baja (# 12) and Santo Tomás de Aquino (# 1). No onchocerciasis seropositive samples were found in 10-year-old individuals or younger. The IgG4 positive titles increased in older participants. CONCLUSIONS: A significant decline in onchocerciasis prevalence was observed in Bioko Island after years of disease-vector control and CDTI strategy. The seroprevalence increased with age, mainly in rural settings that could be due to previous exposure of population to the filarial parasite, eliminated by the control programmes introduced against onchocerciasis. A new Ov-16 serological evaluation with a larger sample size of children below 10 years of age is required to demonstrate the interruption of transmission of O. volvulus in the human population of Bioko Island (Equatorial Guinea) according to the WHO criteria.
[Mh] Termos MeSH primário: Anticorpos Anti-Helmínticos/sangue
Imunoglobulina G/sangue
Onchocerca volvulus/imunologia
Oncocercose Ocular/epidemiologia
Oncocercose/epidemiologia
Estudos Soroepidemiológicos
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Antígenos de Helmintos/imunologia
Criança
Pré-Escolar
Estudos Transversais
Ensaio de Imunoadsorção Enzimática
Guiné Equatorial/epidemiologia
Características da Família
Feminino
Seres Humanos
Insetos Vetores/parasitologia
Ivermectina/uso terapêutico
Masculino
Meia-Idade
Onchocerca volvulus/isolamento & purificação
Oncocercose/tratamento farmacológico
Oncocercose/imunologia
Oncocercose Ocular/tratamento farmacológico
Oncocercose Ocular/imunologia
Oncocercose Ocular/parasitologia
Prevalência
Simuliidae/parasitologia
Adulto Jovem
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Immunoglobulin G); 70288-86-7 (Ivermectin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-016-1779-8


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[PMID]:27604904
[Au] Autor:Campbell B; Cortes H; Annoscia G; Giannelli A; Parisi A; Latrofa MS; Dantas-Torres F; Cardoso L; Otranto D
[Ad] Endereço:Dipartimento di Medicina Veterinaria, Universitá degli Studi di Bari, Bari, Italy. bronwyn.campbell@alumni.unimelb.edu.au.
[Ti] Título:Paramyosin of canine Onchocerca lupi: usefulness for the diagnosis of a neglected zoonotic disease.
[So] Source:Parasit Vectors;9(1):493, 2016 Sep 07.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Of increasing importance to the medical and veterinary communities is the zoonotic filarioid nematode Onchocerca lupi. Onchocercosis, thus far found in wolves, dogs, cats and humans, is diagnosed via skin snips to detect microfilariae and surgical removal of adults from the eye of the host. These methods are time-consuming, laborious and invasive, highlighting the need for new tools for the diagnosis of O. lupi in susceptible hosts. Symptoms related to the presence of the adults in the eye can range from none apparent to severe, including blindness. No reliable chemotherapeutic protocols are available, as yet, to eliminate the infection. Paramyosin, an invertebrate-specific protein, has been well-studied as an allergen, diagnostic marker and vaccine candidate. The aim of this study, therefore, was to isolate and characterise paramyosin from O. lupi to assess its suitability for the development of a serological diagnostic assay. METHODS: The adult and microfilarial stages of O. lupi were isolated from the eyes and skin of a 3-year-old male dog. Total RNA was extracted and reverse transcribed into single stranded cDNA. Reverse-transcription PCR was used to isolate a full-length paramyosin cDNA from adult worms and to investigate the temporal expression patterns of this gene. All amplicons were sequenced using dideoxy chain termination sequencing. Bioinformatics was used to predict the amino acid sequence of the gene, to compare the DNA and protein sequences with those available in public databases and to investigate the phylogenetic relationship of all molecules. Antibody binding sites were predicted using bioinformatics and mapped along with published antigenic epitopes against the O. lupi paramyosin protein. The native protein, and three smaller recombinantly expressed peptides, were subjected to western blot using serum from dogs both positive and negative for O. lupi. RESULTS: Paramyosin of O. lupi was herein molecularly characterized, encoded by a transcript of 2,643 bp and producing a protein of 881 amino acids (101.24 kDa). The paramyosin transcript was detected, by reverse transcription PCR, in adults and microfilariae, but not in eggs. Phylogenetic analysis indicates that this molecule clusters with paramyosins from other filarioids to the exclusion of those from other taxa. A total of 621 unique antibody binding epitopes were predicted for this protein and another 28 were conserved in other organisms. This information was used to design three peptides, for recombinant expression, to identify the antibody binding epitope(s) and reduce potential cross-reactivity with serum from dogs infected with other filarioid nematodes. Native paramyosin, purified from microfilariae and adults, was detected by antibodies present in serum from dogs with known O. lupi infections. CONCLUSIONS: Data provided herein may assist in the development of a serological diagnostic test, based on antibodies to O. lupi paramyosin, for the diagnosis of this infection, in order to gain more information on the real distribution of this little known filarioid of zoonotic concern.
[Mh] Termos MeSH primário: Doenças do Cão/diagnóstico
Doenças Negligenciadas/diagnóstico
Onchocerca/química
Oncocercose Ocular/diagnóstico
Oncocercose/diagnóstico
Tropomiosina/genética
Tropomiosina/imunologia
[Mh] Termos MeSH secundário: Adulto
Animais
Gatos
Biologia Computacional
Doenças do Cão/parasitologia
Cães
Epitopos/imunologia
Seres Humanos
Masculino
Microfilárias/genética
Microfilárias/isolamento & purificação
Doenças Negligenciadas/parasitologia
Onchocerca/imunologia
Onchocerca/isolamento & purificação
Oncocercose/sangue
Oncocercose/imunologia
Oncocercose/parasitologia
Oncocercose Ocular/sangue
Oncocercose Ocular/imunologia
Oncocercose Ocular/parasitologia
Filogenia
Reação em Cadeia da Polimerase
Testes Sorológicos
Tropomiosina/sangue
Tropomiosina/isolamento & purificação
Zoonoses
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Epitopes); 0 (Tropomyosin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171025
[Lr] Data última revisão:
171025
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160909
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-016-1783-z


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[PMID]:27458720
[Au] Autor:Hernando Y; Colwell K; Wright BD
[Ad] Endereço:Centro de Edafología y Biología Aplicada del Segura (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), Espinardo, Murcia, Spain. yhernando@cebas.csic.es.
[Ti] Título:Doing well while fighting river blindness: the alignment of a corporate drug donation programme with responsibilities to shareholders.
[So] Source:Trop Med Int Health;21(10):1304-1310, 2016 Oct.
[Is] ISSN:1365-3156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Using the example of Merck's donations of ivermectin, to show how tax incentives and non-profit collaborators can make corporate largesse consistent with obligations to maximise returns to shareholders. METHODS: We obtained information from publicly available data and estimated Merck's tax deductions according to the US Internal Revenue Code. Reviews of Merck-Kitasato contracts and personal interviews provided additional information regarding key lessons from this collaboration. RESULTS: Our best estimate of the direct cost to Merck of the ivermectin tablets donated during 2005-2011 is around US$ 600 million, well below the stated value of US$ 3.8 billion. Our calculation of tax write-offs reduces the net cost to around US$ 180 million in that period. Indirect market benefits and effects on goodwill further enhanced the compatibility of Merck's donation programme with the company's profit-maximising objective. The case offers lessons for effective management of collaborations with public and non-profit organisations. CONCLUSION: Merck's role in the donation of ivermectin for the treatment of onchocerciasis is widely and justly acknowledged as a prime example of corporate largesse in the public interest. It is nevertheless important to note that several public and non-profit collaborators, and United States taxpayers, played significant roles in increasing Merck's incentives, and indeed ability, to conduct the donation programme that changed so many lives in poor countries, while meeting its responsibilities to shareholders. Overall, the record indicates responsible corporate management of Merck's ivermectin programme and demonstrates the feasibility of socially responsible policies in a manner compatible with obligations to shareholders.
[Mh] Termos MeSH primário: Indústria Farmacêutica/economia
Filaricidas/economia
Ivermectina/economia
Oncocercose Ocular/tratamento farmacológico
[Mh] Termos MeSH secundário: Países em Desenvolvimento
Filaricidas/uso terapêutico
Seres Humanos
Cooperação Internacional
Ivermectina/uso terapêutico
Impostos/estatística & dados numéricos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Filaricides); 70288-86-7 (Ivermectin)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170602
[Lr] Data última revisão:
170602
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160727
[St] Status:MEDLINE
[do] DOI:10.1111/tmi.12759


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[PMID]:27448085
[Au] Autor:Moya L; Herrador Z; Ta-Tang TH; Rubio JM; Perteguer MJ; Hernandez-González A; García B; Nguema R; Nguema J; Ncogo P; Garate T; Benito A; Sima A; Aparicio P
[Ad] Endereço:Jimenez Diaz Foundation, Madrid, Spain.
[Ti] Título:Evidence for Suppression of Onchocerciasis Transmission in Bioko Island, Equatorial Guinea.
[So] Source:PLoS Negl Trop Dis;10(7):e0004829, 2016 Jul.
[Is] ISSN:1935-2735
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Onchocerciasis or "river blindness" is a chronic parasitic neglected tropical disease which is endemic both in mainland and insular Equatorial Guinea. We aim to estimate the current epidemiological situation of onchocerciasis in Bioko Island after vector elimination in 2005 and more than sixteen years of Community Directed Treatment with Ivermectin (CDTI) by using molecular and serological approaches for onchocerciasis diagnosis. A community-based cross-sectional study was carried out in Bioko Island from mid-January to mid-February 2014. A total of 544 study participants were recruited. A complete dermatological examination was performed and three skin snips were performed in every participant for parasitological and molecular assessments. Blood spots were also taken for determination of Ov16 IgG4 antibodies trough an "in-house" ELISA assay. Overall, we found 15 out of 522 individuals suffering any onchocerciasis specific cutaneous lesions and 16 out of 528 (3.0%) with onchocercal nodules in the skin. Nodules were significantly associated with age, being more common in subjects older than 10 years than in younger people (3.9% vs. 0%, p = 0.029). Regarding the onchocerciasis laboratory assessment, no positive parasitological test for microfilaria detection was found in the skin snips. The calculated seroprevalence through IgG4 serology was 7.9%. No children less than 10 years old were found to be positive for this test. Only one case was positive for Onchocerca volvulus (O. volvulus) after skin PCR. The present study points out that the on-going mass ivermectin treatment has been effective in reducing the prevalence of onchocerciasis and corroborates the interruption of transmission in Bioko Island. To our knowledge, this is the first time that accurate information through molecular and serological techniques is generated to estimate the onchocerciasis prevalence in this zone. Sustained support from the national program and appropriate communication and health education strategies to reinforce participation in CDTI activities are essential to ensure progress towards onchocerciasis elimination in the country.
[Mh] Termos MeSH primário: Antiparasitários/administração & dosagem
Ivermectina/administração & dosagem
Onchocerca volvulus/efeitos dos fármacos
Oncocercose Ocular/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Criança
Pré-Escolar
Estudos Transversais
Guiné Equatorial/epidemiologia
Feminino
Seres Humanos
Masculino
Onchocerca volvulus/genética
Onchocerca volvulus/isolamento & purificação
Onchocerca volvulus/fisiologia
Oncocercose Ocular/epidemiologia
Oncocercose Ocular/parasitologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiparasitic Agents); 70288-86-7 (Ivermectin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160723
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0004829


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[PMID]:27216752
[Au] Autor:Lagatie O; Njumbe Ediage E; Batsa Debrah L; Diels L; Nolten C; Vinken P; Debrah A; Dillen L; Silber S; Stuyver LJ
[Ad] Endereço:Janssen Diagnostics, Janssen R&D, Turnhoutseweg 30, 2340, Beerse, Belgium. olagatie@its.jnj.com.
[Ti] Título:Evaluation of the diagnostic potential of urinary N-Acetyltyramine-O,ß-glucuronide (NATOG) as diagnostic biomarker for Onchocerca volvulus infection.
[So] Source:Parasit Vectors;9(1):302, 2016 05 23.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Onchocerciasis, also known as river blindness is one of the neglected tropical diseases affecting millions of people, mainly in sub-Saharan Africa and is caused by the filarial nematode Onchocerca volvulus. Efforts to eliminate this disease are ongoing and are based on mass drug administration programs with the microfilaricide ivermectin. In order to monitor the efficacy of these programs, there is an unmet need for diagnostic tools capable of identifying infected patients. We have investigated the diagnostic potential of urinary N-acetyltyramine-O,ß-glucuronide (NATOG), which is a promising O. volvulus specific biomarker previously identified by urine metabolome analysis. METHODS: A liquid chromatography tandem mass spectrometry (LC-MS/MS) method was used to assess the stability characteristics of NATOG and to evaluate the levels of NATOG in study samples. An LC-fluorescence method was also developed. RESULTS: Stability characteristics of NATOG were investigated and shown to be ideally suited for use in tropical settings. Also, an easy and more accessible method based on liquid chromatography coupled to fluorescence detection was developed and shown to have the necessary sensitivity (limit of quantification 1 µM). Furthermore, we have evaluated the levels of NATOG in a population of 98 nodule-positive individuals from Ghana with no or low levels of microfilaria in the skin and compared them with the levels observed in different control groups (endemic controls (n = 50), non-endemic controls (n = 18) and lymphatic filariasis (n = 51). Only a few (5 %) of nodule-positive individuals showed an increased level (> 10 µM) of NATOG and there was no statistical difference between the nodule-positive individuals and the control groups (P > 0.05). CONCLUSIONS: Results of the present study indicate the limited potential of NATOG as a diagnostic biomarker for O. volvulus infection in amicrofilaridermic individuals.
[Mh] Termos MeSH primário: Glucuronídeos/urina
Onchocerca volvulus/isolamento & purificação
Oncocercose Ocular/diagnóstico
Oncocercose/diagnóstico
Tiramina/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Biomarcadores/urina
Cromatografia Líquida
Feminino
Seres Humanos
Microfilárias
Espectrometria de Massas em Tandem
Tiramina/urina
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Glucuronides); BZB50E9QVY (N-acetyltyramine); X8ZC7V0OX3 (Tyramine)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160525
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-016-1582-6



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