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[PMID]:29320823
[Au] Autor:Shin J; Ahn KS; Suh GH; Kim HJ; Jeong HS; Kim BS; Choi E; Shin SS
[Ad] Endereço:Department of Parasitology, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Korea.
[Ti] Título:First Blindness Cases of Horses Infected with Setaria Digitata (Nematoda: Filarioidea) in the Republic of Korea.
[So] Source:Korean J Parasitol;55(6):667-671, 2017 Dec.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Ocular setariases of cattle were reported but those of equine hosts have never been reported in the Republic of Korea (Korea). We found motile worms in the aqueous humor of 15 horses (Equus spp.) from 12 localities in southern parts of Korea between January 2004 and November 2017. After the affected animals were properly restrained under sedation and local anesthesia, 10 ml disposable syringe with a 16-gauge needle was inserted into the anterior chamber of the affected eye to successfully remove the parasites. The male worm that was found in 7 of the cases showed a pair of lateral appendages near the posterior terminal end of the body. The papillar arrangement was 3 pairs of precloacal, a pair of adcloacal, and 3 pairs of postcloacal papillae, plus a central papilla just in front of the cloaca. The female worms found in the eyes of 8 horses were characterized by the tapering posterior terminal end of the body with a smooth knob. Worms were all identified as Setaria digitata (von Linstow, 1906) by the morphologic characteristics using light and electron microscopic observations. This is the first blindness cases of 15 horses infected with S. digitata (Nematoda: Filarioidea) in Korea.
[Mh] Termos MeSH primário: Cegueira/etiologia
Cegueira/veterinária
Doenças dos Cavalos/etiologia
Doenças dos Cavalos/parasitologia
Cavalos
Procedimentos Cirúrgicos Oftalmológicos/veterinária
Setaria (Nematoide)/isolamento & purificação
Setaríase/complicações
Setaríase/parasitologia
[Mh] Termos MeSH secundário: Animais
Cegueira/cirurgia
Feminino
Doenças dos Cavalos/cirurgia
Masculino
Microscopia Eletrônica
Procedimentos Cirúrgicos Oftalmológicos/métodos
República da Coreia
Setaria (Nematoide)/anatomia & histologia
Setaria (Nematoide)/ultraestrutura
Setaríase/cirurgia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180112
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.6.667


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[PMID]:28351683
[Au] Autor:Senathilake KS; Karunanayake EH; Samarakoon SR; Tennekoon KH; de Silva ED; Adhikari A
[Ad] Endereço:Institute of Biochemistry, Molecular Biology and Biotechnology, University of Colombo, Colombo 03, Sri Lanka.
[Ti] Título:Oleanolic acid from antifilarial triterpene saponins of Dipterocarpus zeylanicus induces oxidative stress and apoptosis in filarial parasite Setaria digitata in vitro.
[So] Source:Exp Parasitol;177:13-21, 2017 Jun.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Absence of a drug that kills adult filarial parasites remains the major challenge in eliminating human lymphatic filariasis (LF); the second leading cause of long-term and permanent disability. Thus, the discovery of novel antifilarial natural products with potent adulticidal activity is an urgent need. In the present study, methanol extracts of leaves, bark and winged seeds of Dipterocarpus zeylanicus (Dipterocarpaceae) were investigated for macro and microfilaricidal activity. Two antifilarial triterpene saponins were isolated from winged seed extracts by bioactivity guided chromatographic separation and identified using Nuclear Magnetic Resonance and mass spectroscopic analysis as oleanolic acid 3-O-ß-D- glucopyranoside (1) (IC50 = 20.54 µM for adult worms, 19.71 µM for microfilariae ) and oleanolic acid 3-O-α-L-arabinopyranoside (2) (IC50 = 29.02 µM for adult worms, 25.99 µM for microfilariae). Acid hydrolysis of both compounds yielded oleanolic acid (3) which was non or least toxic to human peripheral blood mono nuclear cells (Selectivity index = >10) while retaining similar macrofilaricidal (IC50 = 38.4 µM) and microfilaricidal (IC50 = 35.6 µM) activities. In adult female worms treated with 50 and 100 µM doses of oleanolic acid, condensation of nuclear DNA, apoptotic body formation and tissue damage was observed by using Hoechst 33342 staining, TUNEL assay and Hematoxylin and Eosin staining respectively. A dose dependent increase in caspase 3/CED3 activity and decrease in total protein content were also observed in these parasites. A dose dependant DNA fragmentation was observed in adult parasites and microfilariae. Decreased levels of reduced glutathione (GSH) and elevated levels of glutathione S transferase (GST), superoxide dismutase (SOD) and reactive oxygen species (ROS) were also observed in parasites treated with oleanolic acid indicating an oxidative stress mediated apoptotic event. Compound 3/oleanolic acid was thus identified as a potent and safe antifilarial compound in vitro.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Embriófitas/química
Ácido Oleanólico/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Saponinas/química
Setaria (Nematoide)/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Caspase 3/metabolismo
Fragmentação do DNA/efeitos dos fármacos
Relação Dose-Resposta a Droga
Feminino
Marcação In Situ das Extremidades Cortadas
Concentração Inibidora 50
Dose Letal Mediana
Ácido Oleanólico/isolamento & purificação
Casca de Planta/química
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacologia
Folhas de Planta/química
Espécies Reativas de Oxigênio/metabolismo
Sementes/química
Setaria (Nematoide)/citologia
Setaria (Nematoide)/metabolismo
Setaríase/tratamento farmacológico
Setaríase/parasitologia
Coloração e Rotulagem
Triterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Reactive Oxygen Species); 0 (Saponins); 0 (Triterpenes); 6SMK8R7TGJ (Oleanolic Acid); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE


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[PMID]:28030352
[Au] Autor:Priyadarshi P; Dravid P; Sheikh IH; Saxena S; Tandon A; Kaushal DC; Ali S; Kaushal NA
[Ti] Título:Characterization of immunoreactive proteins of Setaria cervi isolated by preparative polyacrylamide gel electrophoresis.
[So] Source:Acta Parasitol;62(1):29-37, 2017 Mar 01.
[Is] ISSN:1896-1851
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:Filarial parasites are complex mixtures of antigenic proteins and characterization of these antigenic molecules is essential to identify the diagnostically important filaria-specific antigens. In the present study, we have fractionated the somatic extracts from adults of Setaria cervi (bovine filarial parasite) on preparative SDS-polyacrylamide gel electrophoresis and tested the immunoreactivity of the separated gel fractions with polyclonal antibodies against filarial excretory-secretory antigens as well as filarial patients sera. The SDS-PAGE analysis of gel eluted fractions revealed 1 protein band in F-1 fraction, 2 protein bands in F-2 fraction and 2-3 protein bands in all other fractions (F3- F11). Seven gel eluted fractions (F1, F2, F3, F4, F5, F6 and F11) showed high ELISA reactivity with the polyclonal antibody (against excretory-secretory antigen) and four of these fractions (F-1, F-2, F3 and F6) exhibited high ELISA reactivity with antibodies present in filarial patient sera. The reactivities of the gel fractions (F1 and F2), recognized by filarial patients sera, were also tested with the monoclonal antibody (detecting the filarial circulating antigen). The F1 and F2 gel eluted fractions were found to have the target antigen of monoclonal antibody as evident by high reactivity with the monoclonal antibody in ELISA and immunoblotting. The S. cervi gel eluted F1 fraction (containing single antigen) could detect antibodies in filarial patients sera and not in non-filarial sera thereby suggesting its usefulness for specific serodiagnosis of human filariasis.
[Mh] Termos MeSH primário: Antígenos de Helmintos/imunologia
Eletroforese em Gel de Poliacrilamida
Proteínas de Helminto/metabolismo
Setaria (Nematoide)/metabolismo
[Mh] Termos MeSH secundário: Animais
Anticorpos Anti-Helmínticos
Ensaio de Imunoadsorção Enzimática/métodos
Feminino
Proteínas de Helminto/genética
Seres Humanos
Soros Imunes
Testes Imunológicos
Masculino
Camundongos
Coelhos
Setaríase/sangue
Setaríase/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Helminth Proteins); 0 (Immune Sera)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170517
[Lr] Data última revisão:
170517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161229
[St] Status:MEDLINE


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[PMID]:27794504
[Au] Autor:Nabie R; Spotin A; Rouhani S
[Ad] Endereço:Nikookari Eye Center, Tabriz University of Medical Sciences, Tabriz, Iran.
[Ti] Título:Subconjunctival setariasis due to Setaria equina infection; a case report and a literature review.
[So] Source:Parasitol Int;66(1):930-932, 2017 Feb.
[Is] ISSN:1873-0329
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A rare case of human subconjunctival setariasis due to Setaria equina infection is reported herein. A 15-years old girl was referred with a 24h history of edema and redness in her left eye. On slit lamp examination, a thread-like cylindrical worm was moving in the subconjunctival area. The worm was extracted, stained and measured 110mm in length 510µm in width. The isolated worm was identified as adult female S. equina based on morphometric criteria. Identification of the species of the worm was confirmed using molecular methods. For this purpose, the 12S rRNA gene was PCR-amplified and the purified amplicon was directly sequenced. After alignment, phylogenetic analysis revealed that the 12S rRNA sequence of this worm (Accession no.: KU291446) showed 100% identity with that of S. equina. This is the first case in Iran and provides evidence that S. equina can be an etiological agent of subconjunctival infection was isolated and diagnosed as where it located Middle East.
[Mh] Termos MeSH primário: Túnica Conjuntiva/parasitologia
Infecções Oculares Parasitárias/parasitologia
Setaria (Nematoide)/isolamento & purificação
Setaríase/parasitologia
[Mh] Termos MeSH secundário: Adolescente
Animais
Infecções Oculares Parasitárias/diagnóstico
Feminino
Seres Humanos
Irã (Geográfico)
Filogenia
Reação em Cadeia da Polimerase
RNA Ribossômico
Alinhamento de Sequência
Análise de Sequência de DNA
Setaríase/diagnóstico
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (RNA, Ribosomal); 0 (RNA, ribosomal, 12S)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170417
[Lr] Data última revisão:
170417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161107
[St] Status:MEDLINE


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[PMID]:27788338
[Au] Autor:Gomez-Puerta LA; Mayor P
[Ad] Endereço:Department of Veterinary Epidemiology and Economics, School of Veterinary Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru.
[Ti] Título:Congenital Filariasis Caused by Setaria bidentata (Nematoda: Filarioidea) in the Red Brocket Deer (Mazama americana).
[So] Source:J Parasitol;103(1):123-126, 2017 Feb.
[Is] ISSN:1937-2345
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The filarial nematode Setaria bidentata was found in 10 of 31 fetuses of the red brocket deer ( Mazama americana ) from the Loreto region of the Peruvian Amazon. A total of 25 specimens were collected and morphologically identified as S. bidentata. Filarial nematodes were found in the peritoneal cavity of 9 deer fetuses and the thoracic cavity of 1 fetus. Most specimens were adult stage. In this report, we provide morphometric data for these filarial specimens. This is the first study to demonstrate prenatal S. bidentata infection in cervid fetuses. Also, the finding of S. bidentata in Peru expands the geographic range of this parasite.
[Mh] Termos MeSH primário: Cervos/parasitologia
Doenças Fetais/veterinária
Setaria (Nematoide)
Setaríase/congênito
[Mh] Termos MeSH secundário: Animais
Cervos/embriologia
Feminino
Doenças Fetais/parasitologia
Masculino
Cavidade Peritoneal/embriologia
Cavidade Peritoneal/parasitologia
Peru
Setaria (Nematoide)/anatomia & histologia
Setaria (Nematoide)/isolamento & purificação
Setaríase/parasitologia
Cavidade Torácica/embriologia
Cavidade Torácica/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170531
[Lr] Data última revisão:
170531
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161028
[St] Status:MEDLINE
[do] DOI:10.1645/16-86


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[PMID]:26965172
[Au] Autor:Singh N; Wadhawan M; Tiwari S; Kumar R; Rathaur S
[Ad] Endereço:Department of Biochemistry, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
[Ti] Título:Inhibition of Setaria cervi protein tyrosine phosphatases by Phenylarsine oxide: A proteomic and biochemical study.
[So] Source:Acta Trop;159:20-8, 2016 Jul.
[Is] ISSN:1873-6254
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Phenylarsine oxide (PAO), a specific protein tyrosine phosphatase (PTP) inhibitor significantly decreased the motility and viability of Setaria cervi ultimately leading to its death. The PTP activity present in the cytosolic and detergent soluble fractions as well as on surface of these parasites was significantly inhibited by PAO. A marked alteration in protein spots abundance after proteomic analysis showed 14 down-regulated and 9 upregulated spots in the treated parasites as compared to the control. The PTP inhibition led to increase in the cytosolic and mitochondrial calpain activity in these parasites. PAO also blocked the ATP generation in the parasite depicted by reduced activity of phosphoglycerate kinase and expression of enolase. An increased ROS level, induced lipid peroxidation/protein carbonyl formation and decreased activity of different antioxidant enzymes like thioredoxin reductase, glutathione reductase and glutathione transferases was also observed in the PAO treated parasites. PAO, thus disturbs the overall homeostasis of the filarial parasite by inhibiting PTPs. Thereby suggesting that these molecules could be used as a good chemotherapeutic target for lymphatic filariasis.
[Mh] Termos MeSH primário: Arsenicais/metabolismo
Inibidores Enzimáticos/metabolismo
Proteínas de Helminto/metabolismo
Proteínas Tirosina Fosfatases/metabolismo
Setaria (Nematoide)/enzimologia
Setaríase/prevenção & controle
Setaríase/parasitologia
[Mh] Termos MeSH secundário: Animais
Búfalos/parasitologia
Índia
Proteômica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arsenicals); 0 (Enzyme Inhibitors); 0 (Helminth Proteins); 0HUR2WY345 (oxophenylarsine); EC 3.1.3.48 (Protein Tyrosine Phosphatases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170130
[Lr] Data última revisão:
170130
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160312
[St] Status:MEDLINE


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[PMID]:26627139
[Au] Autor:Saini P; Saha SK; Roy P; Chowdhury P; Sinha Babu SP
[Ad] Endereço:Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan 731235, West Bengal, India.
[Ti] Título:Evidence of reactive oxygen species (ROS) mediated apoptosis in Setaria cervi induced by green silver nanoparticles from Acacia auriculiformis at a very low dose.
[So] Source:Exp Parasitol;160:39-48, 2016 Jan.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Green synthesis of silver nanomaterial plays a pivotal role in the growing field of nanotechnology. Development of anti-parasitic drugs from plant metabolites has been in regular practice from the ancient period but most of them were discarded due to their inefficiency to control diseases effectively. At present, nanoparticles are used for developing anti-parasitic therapy for their unique properties such as smallest in size, bio-ability, bio-compatibility and penetration capacity into a cell. The present study aims at synthesis of silver nanoparticles (AgNPs) by using funicles extract of Acacia auriculiformis and tests its efficacy as antifilarial. Experimental evidence show that AgNPs are effective at a very low concentration compared to crude plant extracts. Synthesis of these nanoparticles is a single-step, biogenic, cost effective and eco-friendly process. Synthesized nanoparticles were characterized by UV-Vis spectroscopy, TEM, SAED, FTIR, EDX, FESEM and Z-potential. The antifilarial efficacy of AgNPs was tested against different life cycle stages of bovine filarial parasite Setaria cervi by morphological study, motility assessment and viability assay. These nanoparticles are found to have antifilarial activity with LC50 of 5.61 µg/mL and LC90 of 15.54 µg/mL against microfilaria of S. cervi. The microscopic findings and the detailed molecular studies confirmed that green synthesized AgNPs were effective enough to induce apoptosis through up regulation of ROS (reactive oxygen species).
[Mh] Termos MeSH primário: Acacia/química
Apoptose/efeitos dos fármacos
Nanopartículas Metálicas/efeitos adversos
Espécies Reativas de Oxigênio/metabolismo
Setaria (Nematoide)/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Apoptose/fisiologia
Bovinos
Fragmentação do DNA
DNA de Helmintos/química
DNA de Helmintos/genética
Feminino
Marcação In Situ das Extremidades Cortadas
Macrófagos Peritoneais/efeitos dos fármacos
Masculino
Nanopartículas Metálicas/administração & dosagem
Nanopartículas Metálicas/toxicidade
Microscopia Eletrônica de Varredura
Espectroscopia Fotoeletrônica
Extratos Vegetais/química
Ratos
Ratos Wistar
Setaria (Nematoide)/citologia
Setaria (Nematoide)/genética
Setaria (Nematoide)/metabolismo
Setaríase/parasitologia
Prata
Espectroscopia de Infravermelho com Transformada de Fourier
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Helminth); 0 (Plant Extracts); 0 (Reactive Oxygen Species); 3M4G523W1G (Silver)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160109
[Lr] Data última revisão:
160109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151203
[St] Status:MEDLINE


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[PMID]:25924635
[Au] Autor:Perumal AN; Gunawardene YI; Dassanayake RS
[Ad] Endereço:Department of Chemistry,Faculty of Science, University of Colombo,Sri Lanka.
[Ti] Título:Setaria digitata in advancing our knowledge of human lymphatic filariasis.
[So] Source:J Helminthol;90(2):129-38, 2016 Mar.
[Is] ISSN:1475-2697
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Setaria digitata is a filarial parasite that causes fatal cerebrospinal nematodiasis in goats, sheep and horses, resulting in substantial economic losses in animal husbandry in the tropics. Due to its close resemblance to Wuchereria bancrofti, this nematode is also frequently used as a model organism to study human lymphatic filariasis. This review highlights numerous insights into the morphological, histological, biochemical, immunological and genetic aspects of S. digitata that have broadened our understanding towards the control and eradication of filarial diseases.
[Mh] Termos MeSH primário: Filariose Linfática/parasitologia
Setaria (Nematoide)
Setaríase/parasitologia
[Mh] Termos MeSH secundário: Animais
Filariose Linfática/patologia
Filariose Linfática/terapia
Seres Humanos
Setaríase/epidemiologia
Setaríase/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150501
[St] Status:MEDLINE
[do] DOI:10.1017/S0022149X15000309


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[PMID]:26646774
[Au] Autor:Masny A; Salamatin R
[Ad] Endereço:Department of Medical Parasitology, National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland. amasny@gmx.com.
[Ti] Título:Molecular mass screening of mosquitoes for filarial parasites in Germany--re-interpretation of PCR xenomonitoring results would be required.
[So] Source:Parasit Vectors;8:626, 2015 Dec 09.
[Is] ISSN:1756-3305
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Comments concerning interpretation of the PCR xenomonitoring results in the article "Molecular detection of Setaria tundra (Nematoda: Filarioidea) and an unidentified filarial species in mosquitoes in Germany" Parasites & Vectors 2012, 5:14.
[Mh] Termos MeSH primário: Culicidae/parasitologia
Filariose/parasitologia
Filarioidea/isolamento & purificação
Insetos Vetores/parasitologia
Setaria (Nematoide)/isolamento & purificação
Setaríase/parasitologia
[Mh] Termos MeSH secundário: Animais
Feminino
Seres Humanos
[Pt] Tipo de publicação:COMMENT; LETTER; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1512
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151210
[St] Status:MEDLINE
[do] DOI:10.1186/s13071-015-1241-3


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[PMID]:26341797
[Au] Autor:Singh N; Yadav S; Rathaur S
[Ad] Endereço:Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India.
[Ti] Título:Identification and characterization of novel membrane-bound PRL protein tyrosine phosphatases from Setaria cervi, a bovine filarial parasite.
[So] Source:Parasitol Res;114(11):4267-79, 2015 Nov.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A significant amount of protein tyrosine phosphatase (PTP) activity was detected in the detergent-soluble membrane-bound fraction of Setaria cervi, a bovine filarial parasite. The membrane-bound PTP activity was significantly inhibited when the adult parasites were exposed to compounds having antifilarial activity like aspirin and SK7 as well as phenylarsine oxide, a specific PTP inhibitor suggesting that this activity is stress regulated. Further, this enzyme was purified as a single protein of apparently 21 kDa using two different chromatographic techniques. The MALDI-MS/MS analysis of its peptides showed closest match with protein tyrosine phosphatase PRL (Aedes aegypti). This purified enzyme (named as PRL) showed maximum activity at pH 5.5/37 °C and hydrolysed para nitro phenyl phosphate (pNPP) at the highest rate followed by O-P-L-tyrosine and O-P-L-threonine. It showed significant inhibition by specific inhibitors of PTP such as sodium orthovanadate, phenylarsine oxide and ammonium molybdate and was activated by dithiothreitol (DTT). The active site modification studies suggested involvement of cysteine, arginine, histidine and aspartic acid in the catalytic activity of PRL. The activity of S. cervi PRL was also found to be resistant towards the external oxidative stress. Thus, S. cervi PRL could be taken as a potential target for the management of human lymphatic filariasis.
[Mh] Termos MeSH primário: Doenças dos Bovinos/parasitologia
Proteínas de Helminto/química
Proteínas de Helminto/metabolismo
Proteínas Tirosina Fosfatases/química
Proteínas Tirosina Fosfatases/metabolismo
Setaria (Nematoide)/enzimologia
Setaríase/parasitologia
[Mh] Termos MeSH secundário: Animais
Domínio Catalítico
Bovinos
Proteínas de Helminto/genética
Seres Humanos
Cinética
Proteínas Tirosina Fosfatases/genética
Setaria (Nematoide)/química
Setaria (Nematoide)/genética
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Helminth Proteins); EC 3.1.3.48 (Protein Tyrosine Phosphatases)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171007
[Lr] Data última revisão:
171007
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150906
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-015-4667-9



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