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[PMID]: | 28800757 |
[Au] Autor: | Ngouateu Teufack SE; NMbogning Tayo G; Ngangout Alidou M; Yondo J; Djiomene AF; Wabo Poné J; Mbida FM |
[Ad] Endereço: | Research Unit of Biology and Applied Ecology, Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 067, Dschang, Cameroon. |
[Ti] Título: | Anthelminthic properties of Methylene chloride-methanol (1:1) extracts of two Cameroonians medicinal plants on Heligmosomoides bakeri (Nematoda: Heligmosomatidea). |
[So] Source: | BMC Complement Altern Med;17(1):400, 2017 Aug 11. | [Is] ISSN: | 1472-6882 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | BACKGROUND: The resistance of some medico-veterinary parasite strains as well as the unavailability and toxicity of synthetic anthelminthics on humans, animals and the impacts of their residues in the environment have pushed scientists to turn to plants with anthelminthic properties. Hence, the aim of this work was to contribute to the fight against helminths of medical and veterinary importance in general, and also to clear the environment of their free living stages. METHODS: Fresh eggs of Heligmosomoides bakeri were obtained from the faeces of experimentally infected mice. L and L larval stages were obtained after 48 and 72 h of coproculture respectively. Methylene Chloride-Methanol (1:1) extracts of Annona senegalensis and Nauclea latifolia were diluted in DMSO or Tween 80 to prepare the following concentrations: 625, 1250, 2500, 3750 and 5000 µg/ml. The effects of extract solutions were evaluated on the embryonation of eggs, egg hatching and on L and L survival after 48, 10 and 24 h of incubation. Negative controls were 1.5% DMSO, 4% Tween 80 and a mixture of these solvents. The TLC was carried out and the profiles of secondary metabolites were made. RESULTS: Negative controls had no effect on the embryonation, eggs hatching and on larval mortality. However, it was found that, the extracts affected the free living stages of H. bakeri in a concentration-dependant manner. At the highest concentration (5000 µg/ml), the rate of inhibition of embryonation obtained were 20.80%, 38.15% and 84.83% for Methylene Chloride-Methanol of Annona senegalensis (MCM As), Nauclea latifolia (MCM Nl) extracts and mixture of Annona senegalensis and Nauclea latifolia (MCM As-Nl) extract respectively. For egg hatch, the inhibition rate was 16.10%, 46.24% and 87.07% for the above three extracts respectively at the same concentration of 5000 µg/ml. On L and L larval stages after 24 h of exposure to extracts, the mortality rates of 100%, 54.76% and 96.77% against 98%, 51.44% and 100% were obtained for MCM As, MCM Nl and MCM As-Nl respectively at the highest concentration. The Methylene Chloride-Methanol of A.senegalensis, N. latifolia extracts showed the presence of alkaloids except in N. latifolia extract, flavonoids, sterols, triterpens, tanins, polyphenols, anthraquinons, saponins and terpenoids. CONCLUSION: These findings suggest that, the mixture of the two plant extracts showed an additive (synergetic effect) ovicidal effect and a slight larval mortality on L as compared to the effect of MCM As extract alone. These effects were due to the presence ao secondary metabolites identifies in the plant extracts. Thus, they may be used as possible «disinfectants¼ for soil transmitted nematodes. |
[Mh] Termos MeSH primário: |
Annona/química Anti-Helmínticos/farmacologia Larva/efeitos dos fármacos Nematoides/efeitos dos fármacos Infecções por Nematoides/parasitologia Extratos Vegetais/farmacologia Rubiaceae/química
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[Mh] Termos MeSH secundário: |
Animais Camarões Sinergismo Farmacológico Estágios do Ciclo de Vida/efeitos dos fármacos Medicina Tradicional Africana Camundongos Nematoides/crescimento & desenvolvimento Compostos Fitoquímicos/análise Compostos Fitoquímicos/farmacologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Anthelmintics); 0 (Phytochemicals); 0 (Plant Extracts) |
[Em] Mês de entrada: | 1709 |
[Cu] Atualização por classe: | 170901 |
[Lr] Data última revisão:
| 170901 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170813 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1186/s12906-017-1908-8 |
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