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Pesquisa : C03.335.508.700 [Categoria DeCS]
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  1 / 26 MEDLINE  
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[PMID]:28704498
[Au] Autor:Xiang N; Lawrence KS; Kloepper JW; Donald PA; McInroy JA
[Ad] Endereço:Department of Entomology and Plant Pathology, Auburn University, Auburn, Alabama, United States of America.
[Ti] Título:Biological control of Heterodera glycines by spore-forming plant growth-promoting rhizobacteria (PGPR) on soybean.
[So] Source:PLoS One;12(7):e0181201, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Heterodera glycines, the soybean cyst nematode, is the most economically important plant-parasitic nematode on soybean production in the U.S. The objectives of this study were to evaluate the potential of plant growth-promoting rhizobacteria (PGPR) strains for mortality of H. glycines J2 in vitro and for reducing nematode population density on soybean in greenhouse, microplot, and field trials. The major group causing mortality to H. glycines in vitro was the genus Bacillus that consisted of 92.6% of the total 663 PGPR strains evaluated. The subsequent greenhouse, microplot, and field trials indicated that B. velezensis strain Bve2 consistently reduced H. glycines cyst population density at 60 DAP. Bacillus mojavensis strain Bmo3 suppressed H. glycines cyst and total H. glycines population density under greenhouse conditions. Bacillus safensis strain Bsa27 and Mixture 1 (Bve2 + Bal13) reduced H. glycines cyst population density at 60 DAP in the field trials. Bacillus subtilis subsp. subtilis strains Bsssu2 and Bsssu3, and B. velezensis strain Bve12 increased early soybean growth including plant height and plant biomass in the greenhouse trials. Bacillus altitudinis strain Bal13 increased early plant growth on soybean in the greenhouse and microplot trials. Mixture 2 (Abamectin + Bve2 + Bal13) increased early plant growth in the microplot trials at 60 DAP, and also enhanced soybean yield at harvest in the field trials. These results demonstrated that individual PGPR strains and mixtures can reduce H. glycines population density in the greenhouse, microplot, and field conditions, and increased yield of soybean.
[Mh] Termos MeSH primário: Bacillus/fisiologia
Controle Biológico de Vetores/métodos
Doenças das Plantas/prevenção & controle
Raízes de Plantas/microbiologia
Feijão de Soja/microbiologia
Esporos Bacterianos/fisiologia
Tylenchoidea/patogenicidade
[Mh] Termos MeSH secundário: Animais
Antibiose/fisiologia
Doenças das Plantas/parasitologia
Raízes de Plantas/parasitologia
Infecções por Secernentea/prevenção & controle
Feijão de Soja/crescimento & desenvolvimento
Feijão de Soja/parasitologia
Tylenchoidea/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181201


  2 / 26 MEDLINE  
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[PMID]:28655614
[Au] Autor:Dash M; Dutta TK; Phani V; Papolu PK; Shivakumara TN; Rao U
[Ad] Endereço:Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
[Ti] Título:RNAi-mediated disruption of neuropeptide genes, nlp-3 and nlp-12, cause multiple behavioral defects in Meloidogyne incognita.
[So] Source:Biochem Biophys Res Commun;490(3):933-940, 2017 Aug 26.
[Is] ISSN:1090-2104
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Owing to the current deficiencies in chemical control options and unavailability of novel management strategies, root-knot nematode (M. incognita) infections remain widespread with significant socio-economic impacts. Helminth nervous systems are peptide-rich and appear to be putative drug targets that could be exploited by antihelmintic chemotherapy. Herein, to characterize the novel peptidergic neurotransmitters, in silico mining of M. incognita genomic and transciptomic datasets revealed the presence of 16 neuropeptide-like protein (nlp) genes with structural hallmarks of neuropeptide preproproteins; among which 13 nlps were PCR-amplified and sequenced. Two key nlp genes (Mi-nlp-3 and Mi-nlp-12) were localized to the basal bulb and tail region of nematode body via in situ hybridization assay. Mi-nlp-3 and Mi-nlp-12 were greatly expressed (in qRT-PCR assay) in the pre-parasitic juveniles and adult females, suggesting the association of these genes in host recognition, development and reproduction of M. incognita. In vitro knockdown of Mi-nlp-3 and Mi-nlp-12 via RNAi demonstrated the significant reduction in attraction and penetration of M. incognita in tomato root in Pluronic gel medium. A pronounced perturbation in development and reproduction of NLP-silenced worms was also documented in adzuki beans in CYG growth pouches. The deleterious phenotypes obtained due to NLP knockdown suggests that transgenic plants engineered to express RNA constructs targeting nlp genes may emerge as an environmentally viable option to manage nematode problems in crop plants.
[Mh] Termos MeSH primário: Genes de Helmintos
Neuropeptídeos/genética
Doenças das Plantas/parasitologia
Plantas/parasitologia
Interferência de RNA
Infecções por Secernentea/parasitologia
Tylenchoidea/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Genômica
Neuropeptídeos/análise
Neuropeptídeos/metabolismo
Tylenchoidea/química
Tylenchoidea/fisiologia
Tylenchoidea/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuropeptides)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE


  3 / 26 MEDLINE  
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[PMID]:28241060
[Au] Autor:Warnock ND; Wilson L; Patten C; Fleming CC; Maule AG; Dalzell JJ
[Ad] Endereço:Microbes & Pathogen Biology, The Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, United Kingdom.
[Ti] Título:Nematode neuropeptides as transgenic nematicides.
[So] Source:PLoS Pathog;13(2):e1006237, 2017 Feb.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Plant parasitic nematodes (PPNs) seriously threaten global food security. Conventionally an integrated approach to PPN management has relied heavily on carbamate, organophosphate and fumigant nematicides which are now being withdrawn over environmental health and safety concerns. This progressive withdrawal has left a significant shortcoming in our ability to manage these economically important parasites, and highlights the need for novel and robust control methods. Nematodes can assimilate exogenous peptides through retrograde transport along the chemosensory amphid neurons. Peptides can accumulate within cells of the central nerve ring and can elicit physiological effects when released to interact with receptors on adjoining cells. We have profiled bioactive neuropeptides from the neuropeptide-like protein (NLP) family of PPNs as novel nematicides, and have identified numerous discrete NLPs that negatively impact chemosensation, host invasion and stylet thrusting of the root knot nematode Meloidogyne incognita and the potato cyst nematode Globodera pallida. Transgenic secretion of these peptides from the rhizobacterium, Bacillus subtilis, and the terrestrial microalgae Chlamydomonas reinhardtii reduce tomato infection levels by up to 90% when compared with controls. These data pave the way for the exploitation of nematode neuropeptides as a novel class of plant protective nematicide, using novel non-food transgenic delivery systems which could be deployed on farmer-preferred cultivars.
[Mh] Termos MeSH primário: Antinematódeos/farmacologia
Neuropeptídeos/farmacologia
Controle Biológico de Vetores/métodos
Doenças das Plantas/parasitologia
Infecções por Secernentea
[Mh] Termos MeSH secundário: Animais
Organismos Geneticamente Modificados
Microbiologia do Solo
Tylenchoidea
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antinematodal Agents); 0 (Neuropeptides)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006237


  4 / 26 MEDLINE  
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[PMID]:26940543
[Au] Autor:Janssen T; Karssen G; Verhaeven M; Coyne D; Bert W
[Ad] Endereço:Nematology Research Unit, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, 9000 Ghent, Belgium.
[Ti] Título:Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolution.
[So] Source:Sci Rep;6:22591, 2016 Mar 04.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The polyphagous parthenogenetic root-knot nematodes of the genus Meloidogyne are considered to be the most significant nematode pest in sub-tropical and tropical agriculture. Despite the crucial need for correct diagnosis, identification of these pathogens remains problematic. The traditionally used diagnostic strategies, including morphometrics, host-range tests, biochemical and molecular techniques, now appear to be unreliable due to the recently-suggested hybrid origin of root-knot nematodes. In order to determine a suitable barcode region for these pathogens nine quickly-evolving mitochondrial coding genes were screened. Resulting haplotype networks revealed closely related lineages indicating a recent speciation, an anthropogenic-aided distribution through agricultural practices, and evidence for reticulate evolution within M. arenaria. Nonetheless, nucleotide polymorphisms harbor enough variation to distinguish these closely-related lineages. Furthermore, completeness of lineage sorting was verified by screening 80 populations from widespread geographical origins and variable hosts. Importantly, our results indicate that mitochondrial haplotypes are strongly linked and consistent with traditional esterase isozyme patterns, suggesting that different parthenogenetic lineages can be reliably identified using mitochondrial haplotypes. The study indicates that the barcode region Nad5 can reliably identify the major lineages of tropical root-knot nematodes.
[Mh] Termos MeSH primário: Evolução Biológica
DNA Mitocondrial/genética
Redes Reguladoras de Genes
Infecções por Secernentea/diagnóstico
Tylenchoidea/genética
[Mh] Termos MeSH secundário: Animais
Código de Barras de DNA Taxonômico
Esterases/genética
Genes de Helmintos/genética
Especiação Genética
Haplótipos
Seres Humanos
Partenogênese/genética
Raízes de Plantas
Plantas
Clima Tropical
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Mitochondrial); EC 3.1.- (Esterases)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160305
[St] Status:MEDLINE
[do] DOI:10.1038/srep22591


  5 / 26 MEDLINE  
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[PMID]:26685616
[Au] Autor:Wang XY; Wang XF; Wei M; Shi QH; Yang FJ
[Ti] Título:[Eupolyphaga frass and its extracts protected tomato from Meloidogyne incognita infestation].
[So] Source:Ying Yong Sheng Tai Xue Bao;26(8):2511-7, 2015 Aug.
[Is] ISSN:1001-9332
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:The control effects of Eupolyphaga (Eupolyphaga sinensis Walker) frass and its extracts on Meloidogyne incognita were studied through laboratory assays and pot experiments, and the organic volatile compounds and oligochitosan contents in the frass were analyzed. The results indicated that the nematode immobility and mortality was significantly increased with increasing the extract concentration and treatment time. Compared with the control, egg hatching was significantly inhibited when the extract concentration was beyond 20%. Pot experiment indicated that root galling of tomato seedlings was reduced and the relative control effect was significantly improved with the increasing frass application. Meanwhile, plant height, stem diameter and leaf number of tomato increased with the increasing dosage of eupolyphaga frass. The ingredients analysis showed that the content of oligochitosan was about 4.35% and there were 9 categories and 110 kinds of volatile compounds in the frass.
[Mh] Termos MeSH primário: Baratas
Lycopersicon esculentum/parasitologia
Controle Biológico de Vetores
Doenças das Plantas/prevenção & controle
Infecções por Secernentea/prevenção & controle
Tylenchoidea
[Mh] Termos MeSH secundário: Animais
Doenças das Plantas/parasitologia
Raízes de Plantas/parasitologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Mês de entrada:1603
[Cu] Atualização por classe:161018
[Lr] Data última revisão:
161018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151222
[St] Status:MEDLINE


  6 / 26 MEDLINE  
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[PMID]:26518548
[Au] Autor:Lavrova VV; Matveeva EM; Zinovieva SV
[Ad] Endereço:Institute of Biology, Karelian Research Center, Russian Academy of Sciences, ul. Pushkinskaya 11, Petrozavodsk, Karelia, 185910, Russia. vvlavrova@mail.ru.
[Ti] Título:The expression of R genes in genetic and induced resistance to potato cyst nematode Globodera rostochiensis (Wollenweber, 1923) Behrens, 1975.
[So] Source:Dokl Biochem Biophys;464:283-5, 2015.
[Is] ISSN:1608-3091
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:The characteristics of expression of two genes, H1 and Gro1-4, which determine the resistance to the sedentary parasitic nematode Globodera rostochiensis (Wollenweber, 1923) Behrens, 1975, in the resistant (Krepysh) and susceptible (Nevskii) potato cultivars was studied under a short-term exposure to low temperatures. Such treatment of susceptible plants at the early stages of ontogeny led to the activation of expression of H1 and Gro1-4 genes in roots and the H1 gene in leaves. The transcriptional activity of R genes was detected not only in roots but also in leaves (i.e., in tissue remote from the site of direct injury by the nematode) in the case of both genetic and induced resistance, indicating the development of a systemic defense response of plants to infection.
[Mh] Termos MeSH primário: Genes de Plantas
Doenças das Plantas/genética
Doenças das Plantas/parasitologia
Infecções por Secernentea/metabolismo
Solanum tuberosum/metabolismo
Tylenchoidea
[Mh] Termos MeSH secundário: Animais
Temperatura Baixa
Expressão Gênica
Predisposição Genética para Doença
Folhas de Planta/genética
Folhas de Planta/metabolismo
Raízes de Plantas/genética
Raízes de Plantas/metabolismo
RNA Mensageiro/metabolismo
Infecções por Secernentea/genética
Solanum tuberosum/genética
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Messenger)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151101
[St] Status:MEDLINE
[do] DOI:10.1134/S160767291505004X


  7 / 26 MEDLINE  
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[PMID]:26363887
[Au] Autor:Begum S; Ayub A; Shaheen Siddiqui B; Fayyaz S; Kazi F
[Ad] Endereço:H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan, (phone: +92-21-99261701-2; fax: +92-21-99261713). dr.sabirabegum@yahoo.com.
[Ti] Título:Nematicidal triterpenoids from Lantana camara.
[So] Source:Chem Biodivers;12(9):1435-42, 2015 Sep.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:A new triterpene, lancamarolide (1), and seven known triterpenes, oleanonic acid (2), lantadene A (3), 11α-hydroxy-3-oxours-12-en-28-oic acid (4), betulinic acid (5), lantadene B (6), and lantaninilic acid (7) were isolated from the aerial parts of Lantana camara in the course of bioassay-guided isolation, and their nematicidal activities against Meloidogyne incognita, the root knot nematode, were carried out. Oleanonic acid was found to be the most active compound and exhibited 80% mortality after 72 h at 0.0625% concentration, which is comparable with that of the standard furadan.
[Mh] Termos MeSH primário: Antinematódeos/química
Antinematódeos/farmacologia
Lantana/química
Plantas/parasitologia
Triterpenos/química
Triterpenos/farmacologia
Tylenchoidea/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Antinematódeos/isolamento & purificação
Seres Humanos
Infecções por Secernentea/tratamento farmacológico
Triterpenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antinematodal Agents); 0 (Triterpenes); 0 (oleanonic acid)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:150914
[Lr] Data última revisão:
150914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150914
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201400460


  8 / 26 MEDLINE  
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[PMID]:25849382
[Au] Autor:Yang X; Wang X; Wang K; Su L; Li H; Li R; Shen Q
[Ad] Endereço:Jiangsu Key Lab and Engineering Center for Solid Organic Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, China.
[Ti] Título:The nematicidal effect of camellia seed cake on root-knot nematode Meloidogyne javanica of banana.
[So] Source:PLoS One;10(4):e0119700, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Suppression of root-knot nematodes is crucially important for maintaining the worldwide development of the banana industry. Growing concerns about human and environmental safety have led to the withdrawal of commonly used nematicides and soil fumigants, thus motivating the development of alternative nematode management strategies. In this study, Meloidogyne javanica was isolated, and the nematicidal effect of Camellia seed cake on this pest was investigated. The results showed that in dish experiments, Camellia seed cake extracts under low concentration (2 g/L) showed a strong nematicidal effect. After treatment for 72 h, the eggs of M. javanica were gradually dissolved, and the intestine of the juveniles gradually became indistinct. Nematicidal compounds, including saponins identified by HPLC-ESI-MS and 8 types of volatile compounds identified by GC-MS, exhibited effective nematicidal activities, especially 4-methylphenol. The pot experiments demonstrated that the application of Camellia seed cake suppressed M. javanica, and promoted the banana plant growth. This study explored an effective nematicidal agent for application in soil and revealed its potential mechanism of nematode suppression.
[Mh] Termos MeSH primário: Antinematódeos/farmacologia
Camellia/química
Doenças das Plantas/prevenção & controle
Saponinas/farmacologia
Infecções por Secernentea/prevenção & controle
Sementes/química
Tylenchoidea/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão
Cromatografia Gasosa-Espectrometria de Massas
Seres Humanos
Musa/parasitologia
Controle Biológico de Vetores
Doenças das Plantas/parasitologia
Extratos Vegetais/farmacologia
Raízes de Plantas/parasitologia
Saponinas/isolamento & purificação
Infecções por Secernentea/parasitologia
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antinematodal Agents); 0 (Plant Extracts); 0 (Saponins)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150408
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0119700


  9 / 26 MEDLINE  
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[PMID]:25822722
[Au] Autor:Postnikova OA; Hult M; Shao J; Skantar A; Nemchinov LG
[Ti] Título:Transcriptome analysis of resistant and susceptible alfalfa cultivars infected with root-knot nematode Meloidogyne incognita.
[So] Source:PLoS One;10(3):e0123157, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nematodes are one of the major limiting factors in alfalfa production. Root-knot nematodes (RKN, Meloidogyne spp.) are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. As of today, no studies have been published on global gene expression profiling in alfalfa infected with RKN or any other plant parasitic nematode. Very little information is available about molecular mechanisms that contribute to pathogenesis and defense responses in alfalfa against these pests and specifically against RKN. In this work, we performed root transcriptome analysis of resistant (cv. Moapa 69) and susceptible (cv. Lahontan) alfalfa cultivars infected with RKN Meloidogyne incognita, widespread root-knot nematode species and a major pest worldwide. A total of 1,701,622,580 pair-end reads were generated on an Illumina Hi-Seq 2000 platform from the roots of both cultivars and assembled into 45,595 and 47,590 transcripts in cvs Moapa 69 and Lahontan, respectively. Bioinformatic analysis revealed a number of common and unique genes that were differentially expressed in susceptible and resistant lines as a result of nematode infection. Although the susceptible cultivar showed a more pronounced defense response to the infection, feeding sites were successfully established in its roots. Characteristically, basal gene expression levels under normal conditions differed between the two cultivars as well, which may confer advantage to one of the genotypes toward resistance to nematodes. Differentially expressed genes were subsequently assigned to known Gene Ontology categories to predict their functional roles and associated biological processes. Real-time PCR validated expression changes in genes arbitrarily selected for experimental confirmation. Candidate genes that contribute to protection against M. incognita in alfalfa were proposed and alfalfa-nematode interactions with respect to resistance are discussed.
[Mh] Termos MeSH primário: Medicago sativa/genética
Medicago sativa/parasitologia
Raízes de Plantas/genética
Raízes de Plantas/parasitologia
Transcriptoma/genética
Tylenchoidea/parasitologia
[Mh] Termos MeSH secundário: Animais
Biologia Computacional
Perfilação da Expressão Gênica/métodos
Doenças das Plantas/genética
Doenças das Plantas/parasitologia
Infecções por Secernentea/genética
Infecções por Secernentea/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150521
[Lr] Data última revisão:
150521
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150331
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0123157


  10 / 26 MEDLINE  
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[PMID]:25255291
[Au] Autor:Eves-van den Akker S; Lilley CJ; Jones JT; Urwin PE
[Ad] Endereço:Centre for Plant Sciences, University of Leeds, Leeds, United Kingdom; Cell and Molecular Sciences Group, Dundee Effector Consortium, James Hutton Institute, Invergowrie, Dundee, United Kingdom.
[Ti] Título:Identification and characterisation of a hyper-variable apoplastic effector gene family of the potato cyst nematodes.
[So] Source:PLoS Pathog;10(9):e1004391, 2014 Sep.
[Is] ISSN:1553-7374
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sedentary endoparasitic nematodes are obligate biotrophs that modify host root tissues, using a suite of effector proteins to create and maintain a feeding site that is their sole source of nutrition. Using assumptions about the characteristics of genes involved in plant-nematode biotrophic interactions to inform the identification strategy, we provide a description and characterisation of a novel group of hyper-variable extracellular effectors termed HYP, from the potato cyst nematode Globodera pallida. HYP effectors comprise a large gene family, with a modular structure, and have unparalleled diversity between individuals of the same population: no two nematodes tested had the same genetic complement of HYP effectors. Individuals vary in the number, size, and type of effector subfamilies. HYP effectors are expressed throughout the biotrophic stages in large secretory cells associated with the amphids of parasitic stage nematodes as confirmed by in situ hybridisation. The encoded proteins are secreted into the host roots where they are detectable by immunochemistry in the apoplasm, between the anterior end of the nematode and the feeding site. We have identified HYP effectors in three genera of plant parasitic nematodes capable of infecting a broad range of mono- and dicotyledon crop species. In planta RNAi targeted to all members of the effector family causes a reduction in successful parasitism.
[Mh] Termos MeSH primário: Proteínas de Helminto/genética
Interações Hospedeiro-Parasita
Doenças das Plantas/parasitologia
Solanum tuberosum/genética
Tylenchoidea/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Animais
Parede Celular/metabolismo
Clonagem Molecular
Biologia Computacional
Variações do Número de Cópias de DNA
DNA de Helmintos/genética
Proteínas de Helminto/metabolismo
Sequenciamento de Nucleotídeos em Larga Escala
Immunoblotting
Hibridização In Situ
Estágios do Ciclo de Vida/genética
Dados de Sequência Molecular
Família Multigênica
Células Vegetais/metabolismo
Doenças das Plantas/genética
Raízes de Plantas/química
Raízes de Plantas/parasitologia
Infecções por Secernentea/genética
Infecções por Secernentea/metabolismo
Infecções por Secernentea/parasitologia
Homologia de Sequência de Aminoácidos
Solanum tuberosum/citologia
Solanum tuberosum/parasitologia
Tylenchoidea/crescimento & desenvolvimento
Tylenchoidea/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Helminth); 0 (Helminth Proteins)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140926
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1004391



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