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[PMID]:28793025
[Au] Autor:Cardoso BA; Fonseca FO; Moraes AHA; Martins ACGS; Oliveira NVDS; Lima LNGC; Dias GADS; Saad MHF
[Ad] Endereço:Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Microbiologia Celular, Rio de Janeiro, Rio de Janeiro, Brazil.
[Ti] Título:Environmental aspects related to tuberculosis and intestinal parasites in a low-income community of the Brazilian Amazon.
[So] Source:Rev Inst Med Trop Sao Paulo;59:e57, 2017 Aug 07.
[Is] ISSN:1678-9946
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:We carried out a cross-sectional study from January to December 2015 on 1,425 inhabitants from a floating population in the Brazilian Amazon (Murinin district, Pará State) to describe the population-based prevalence of tuberculosis (TB) from 2011 to 2014, recent TB contacts (rCts) latently infected with Mycobacterium tuberculosis (LTBI) , the coverage of the local health network, socio-environmental factors, and frequency of intestinal parasitic infection (IPI). We found that the sanitary structure was inadequate, with latrines being shared with other rooms within the same accommodation; well water was the main source of water, and 48% of families had low incomes. The average rate of TB was 105/100, 000 inhabitants per year; one third of TB patients had been household contacts of infected individuals in the past, and 23% of rCts were LTBI. More than half (65%) of 44% of the stools examined (representing 76% of the housing) had IPIs; the highest prevalence was of fecal-oral transmitted protozoa (40%, Giardia intestinalis ), followed by soil-transmitted helminths (23%). TB transmission may be related to insufficient disease control of rCts, frequent relocation, and underreporting. Education, adopting hygienic habits, improving sanitation, provision of a treated water supply and efficient sewage system, further comprehensive epidemiological surveillance of those who enter and leave the community and resources for basic treatment of IPIs are crucial in combating the transmission of these neglected diseases.
[Mh] Termos MeSH primário: Helmintíase/epidemiologia
Enteropatias Parasitárias/epidemiologia
Infecções por Protozoários/epidemiologia
Tuberculose/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Brasil/epidemiologia
Criança
Pré-Escolar
Estudos Transversais
Fezes/parasitologia
Feminino
Helmintíase/diagnóstico
Helmintíase/parasitologia
Seres Humanos
Lactente
Recém-Nascido
Enteropatias Parasitárias/diagnóstico
Enteropatias Parasitárias/parasitologia
Masculino
Meia-Idade
Pobreza
Prevalência
Infecções por Protozoários/diagnóstico
Infecções por Protozoários/parasitologia
Tuberculose/diagnóstico
População Urbana
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE


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[PMID]:28793024
[Au] Autor:Ignacio CF; Silva MECD; Handam NB; Alencar MFL; Sotero-Martins A; Barata MML; Moraes AHA
[Ad] Endereço:Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Inovações em Terapias, Ensino e Bioprodutos, Rio de Janeiro, Rio de Janeiro, Brazil.
[Ti] Título:Socioenvironmental conditions and intestinal parasitic infections in Brazilian urban slums: a cross-sectional study.
[So] Source:Rev Inst Med Trop Sao Paulo;59:e56, 2017 Aug 07.
[Is] ISSN:1678-9946
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Methods: A cross-sectional study covering an agglomeration of urban slums was conducted between 2015 and 2016 using participants observation, a socioeconomic survey, and the spontaneous sedimentation method with three slides per sample to analyze fresh stool specimens ( n =595) searching for intestinal parasites. Results: Endolimax nana ( n =95, 16.0%) and Entamoeba coli ( n =65, 10.9%) were the most frequently identified agents, followed by Giardia intestinalis ( n =24, 4.0%) and Ascaris lumbricoides ( n =11, 1.8%). Coinfections caused by E. nana and E. histolytica/dispar and by Entamoeba coli/A. lumbricoides were significant. The use of piped water as drinking water, the presence of A. lumbricoides , and contamination with coliform bacteria and Escherichia coli were more common in major area (MA) 1. Children (0-19 years) had a greater chance of living in poverty (OR 3.36; 95% CI: 2.50- 4.52; p <0.001) which was pervasive. The predominance of protozoa parasites suggests that a one-size-fits-all approach focusing on preventive chemotherapy for soil-transmitted helminths is not appropriate for all communities in developing countries. It is important that both residents and health professionals consider the socioenvironmental conditions of urban slums when assessing intestinal parasitic infections for disease control and health promotion initiatives.
[Mh] Termos MeSH primário: Helmintíase/epidemiologia
Enteropatias Parasitárias/epidemiologia
Infecções por Protozoários/epidemiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Animais
Brasil/epidemiologia
Criança
Pré-Escolar
Estudos Transversais
Feminino
Helmintíase/parasitologia
Seres Humanos
Lactente
Recém-Nascido
Enteropatias Parasitárias/parasitologia
Masculino
Meia-Idade
Áreas de Pobreza
Prevalência
Infecções por Protozoários/parasitologia
População Urbana
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE


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[PMID]:28771238
[Au] Autor:Cringoli G; Maurelli MP; Levecke B; Bosco A; Vercruysse J; Utzinger J; Rinaldi L
[Ad] Endereço:Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, CREMOPAR, Naples, Italy.
[Ti] Título:The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals.
[So] Source:Nat Protoc;12(9):1723-1732, 2017 Sep.
[Is] ISSN:1750-2799
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This protocol is an extension to: Nat. Protoc. 5, 503-515 (2010); doi: 10.1038/nprot.2009.235; published online 25 February 2010The FLOTAC is a sensitive, accurate, and precise technique for the diagnosis of protozoan and helminth infections in humans and animals. However, it requires centrifugation, and hence might be out of reach in resource-constrained settings. As an extension of the original FLOTAC protocol, this protocol describes the Mini-FLOTAC technique, a logical evolution of FLOTAC conceived to perform multivalent, qualitative, and quantitative diagnosis of helminth and protozoan infections in human and animal feces, and urine. This has been found to be of most use in the processing of large numbers of samples with rapid laboratory workup, and for veterinary applications directly on-farm. In addition to the Mini-FLOTAC apparatus, we describe the use of the Fill-FLOTAC, a closed system used to facilitate the performance of the first four consecutive steps of the Mini-FLOTAC technique: fecal sample collection and weighing, homogenization, filtration, and filling of the Mini-FLOTAC chambers. Processing of an individual sample using this protocol requires ∼12 min.
[Mh] Termos MeSH primário: Técnicas e Procedimentos Diagnósticos/instrumentação
Helmintíase/diagnóstico
Infecções por Protozoários/diagnóstico
[Mh] Termos MeSH secundário: Animais
Técnicas e Procedimentos Diagnósticos/economia
Fezes/microbiologia
Fezes/parasitologia
Seres Humanos
Sensibilidade e Especificidade
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1038/nprot.2017.067


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[PMID]:28653489
[Au] Autor:Berendes D; Leon J; Kirby A; Clennon J; Raj S; Yakubu H; Robb K; Kartikeyan A; Hemavathy P; Gunasekaran A; Roy S; Ghale BC; Kumar JS; Mohan VR; Kang G; Moe C
[Ad] Endereço:School of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
[Ti] Título:Household sanitation is associated with lower risk of bacterial and protozoal enteric infections, but not viral infections and diarrhoea, in a cohort study in a low-income urban neighbourhood in Vellore, India.
[So] Source:Trop Med Int Health;22(9):1119-1129, 2017 Sep.
[Is] ISSN:1365-3156
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: This study examined associations between household sanitation and enteric infection - including diarrhoeal-specific outcomes - in children 0-2 years of age in a low-income, dense urban neighbourhood. METHODS: As part of the MAL-ED study, 230 children in a low-income, urban, Indian neighbourhood provided stool specimens at 14-17 scheduled time points and during diarrhoeal episodes in the first 2 years of life that were analysed for bacterial, parasitic (protozoa and helminths) and viral pathogens. From interviews with caregivers in 100 households, the relationship between the presence (and discharge) of household sanitation facilities and any, pathogen-specific, and diarrhoea-specific enteric infection was tested through mixed-effects Poisson regression models. RESULTS: Few study households (33%) reported having toilets, most of which (82%) discharged into open drains. Controlling for season and household socio-economic status, the presence of a household toilet was associated with lower risks of enteric infection (RR: 0.91, 95% CI: 0.79-1.06), bacterial infection (RR: 0.87, 95% CI: 0.75-1.02) and protozoal infection (RR: 0.64, 95% CI: 0.39-1.04), although not statistically significant, but had no association with diarrhoea (RR: 1.00, 95% CI: 0.68-1.45) or viral infections (RR: 1.12, 95% CI: 0.79-1.60). Models also suggested that the relationship between household toilets discharging to drains and enteric infection risk may vary by season. CONCLUSIONS: The presence of a household toilet was associated with lower risk of bacterial and protozoal enteric infections, but not diarrhoea or viral infections, suggesting the health effects of sanitation may be more accurately estimated using outcome measures that account for aetiologic agents.
[Mh] Termos MeSH primário: Infecções Bacterianas/prevenção & controle
Diarreia
Enteropatias/prevenção & controle
Pobreza
Infecções por Protozoários/prevenção & controle
Sanitários Públicos
Viroses
[Mh] Termos MeSH secundário: Adulto
Animais
Infecções Bacterianas/etiologia
Infecções Bacterianas/microbiologia
Pré-Escolar
Estudos de Coortes
Diarreia/etiologia
Disenteria/etiologia
Disenteria/prevenção & controle
Fezes
Helmintíase/etiologia
Helmintíase/prevenção & controle
Helmintos
Seres Humanos
Renda
Índia
Lactente
Recém-Nascido
Enteropatias/etiologia
Enteropatias/microbiologia
Enteropatias/parasitologia
Infecções por Protozoários/etiologia
Infecções por Protozoários/parasitologia
Distribuição Espacial da População
Fatores de Risco
Saneamento
População Urbana
Viroses/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170628
[St] Status:MEDLINE
[do] DOI:10.1111/tmi.12915


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[PMID]:28645659
[Au] Autor:Colín-Lozano B; León-Rivera I; Chan-Bacab MJ; Ortega-Morales BO; Moo-Puc R; López-Guerrero V; Hernández-Núñez E; Argüello-Garcia R; Scior T; Barbosa-Cabrera E; Navarrete-Vázquez G
[Ad] Endereço:Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos 62209, Mexico.
[Ti] Título:Synthesis, in vitro and in vivo giardicidal activity of nitrothiazole-NSAID chimeras displaying broad antiprotozoal spectrum.
[So] Source:Bioorg Med Chem Lett;27(15):3490-3494, 2017 08 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We designed and synthesized five new 5-nitrothiazole-NSAID chimeras as analogues of nitazoxanide, using a DCC-activated amidation. Compounds 1-5 were tested in vitro against a panel of five protozoa: 2 amitochondriates (Giardia intestinalis, Trichomonas vaginalis) and 3 kinetoplastids (Leishmania mexicana, Leishmania amazonensis and Trypanosoma cruzi). All chimeras showed broad spectrum and potent antiprotozoal activities, with IC values ranging from the low micromolar to nanomolar order. Compounds 1-5 were even more active than metronidazole and nitazoxanide, two marketed first-line drugs against giardiasis. In particular, compound 4 (an indomethacin hybrid) was one of the most potent of the series, inhibiting G. intestinalis growth in vitro with an IC of 0.145µM. Compound 4 was 38-times more potent than metronidazole and 8-times more active than nitazoxanide. The in vivo giardicidal effect of 4 was evaluated in a CD-1 mouse model obtaining a median effective dose of 1.709µg/kg (3.53nmol/kg), a 321-fold and 1015-fold increase in effectiveness after intragastric administration over metronidazole and nitazoxanide, respectively. Compounds 1 and 3 (hybrids of ibuprofen and clofibric acid), showed potent giardicidal activities in the in vitro as well as in the in vivo assays after oral administration. Therefore, compounds 1-5 constitute promising drug candidates for further testing in experimental chemotherapy against giardiasis, trichomoniasis, leishmaniasis and even trypanosomiasis infections.
[Mh] Termos MeSH primário: Antiprotozoários/química
Antiprotozoários/uso terapêutico
Giardia lamblia/efeitos dos fármacos
Giardíase/tratamento farmacológico
Tiazóis/química
Tiazóis/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios não Esteroides/síntese química
Anti-Inflamatórios não Esteroides/química
Anti-Inflamatórios não Esteroides/farmacologia
Anti-Inflamatórios não Esteroides/uso terapêutico
Antiprotozoários/síntese química
Antiprotozoários/farmacologia
Desenho de Drogas
Feminino
Seres Humanos
Leishmania/efeitos dos fármacos
Camundongos
Infecções por Protozoários/tratamento farmacológico
Tiazóis/síntese química
Tiazóis/farmacologia
Trichomonas vaginalis/efeitos dos fármacos
Trypanosoma cruzi/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (5-nitrothiazole); 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Antiprotozoal Agents); 0 (Thiazoles); SOA12P041N (nitazoxanide)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170625
[St] Status:MEDLINE


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[PMID]:28590590
[Au] Autor:Dichiara M; Marrazzo A; Prezzavento O; Collina S; Rescifina A; Amata E
[Ad] Endereço:Department of Drug Sciences, University of Catania, V.le A. Doria, 6, 95100, Catania, Italy.
[Ti] Título:Repurposing of Human Kinase Inhibitors in Neglected Protozoan Diseases.
[So] Source:ChemMedChem;12(16):1235-1253, 2017 Aug 22.
[Is] ISSN:1860-7187
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Human African trypanosomiasis (HAT), Chagas disease, and leishmaniasis belong to a group of infectious diseases known as neglected tropical diseases and are induced by infection with protozoan parasites named trypanosomatids. Drugs in current use have several limitations, and therefore new candidate drugs are required. The majority of current therapeutic trypanosomatid targets are enzymes or cell-surface receptors. Among these, eukaryotic protein kinases are a major group of protein targets whose modulation may be beneficial for the treatment of neglected tropical protozoan diseases. This review summarizes the finding of new hit compounds for neglected tropical protozoan diseases, by repurposing known human kinase inhibitors on trypanosomatids. Kinase inhibitors are grouped by human kinase family and discussed according to the screening (target-based or phenotypic) reported for these compounds on trypanosomatids. This collection aims to provide insight into repurposed human kinase inhibitors and their importance in the development of new chemical entities with potential beneficial effects on the diseases caused by trypanosomatids.
[Mh] Termos MeSH primário: Reposicionamento de Medicamentos
Doenças Negligenciadas/tratamento farmacológico
Inibidores de Proteínas Quinases/uso terapêutico
Proteínas Serina-Treonina Quinases/antagonistas & inibidores
Infecções por Protozoários/tratamento farmacológico
[Mh] Termos MeSH secundário: Seres Humanos
Doenças Negligenciadas/prevenção & controle
Inibidores de Proteínas Quinases/química
Proteínas Serina-Treonina Quinases/metabolismo
Infecções por Protozoários/prevenção & controle
Vacinas Protozoárias/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Protein Kinase Inhibitors); 0 (Protozoan Vaccines); EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1002/cmdc.201700259


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[PMID]:28584161
[Au] Autor:Burgess SL; Gilchrist CA; Lynn TC; Petri WA
[Ad] Endereço:Division of Infectious Diseases and International Health, Department of Medicine, University of Virginia, Charlottesville, Virginia, USA.
[Ti] Título:Parasitic Protozoa and Interactions with the Host Intestinal Microbiota.
[So] Source:Infect Immun;85(8), 2017 Aug.
[Is] ISSN:1098-5522
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Parasitic protozoan infections represent a major health burden in the developing world and contribute significantly to morbidity and mortality. These infections are often associated with considerable variability in clinical presentation. An emerging body of work suggests that the intestinal microbiota may help to explain some of these differences in disease expression. The objective of this minireview is to synthesize recent progress in this rapidly advancing field. Studies of humans and animals and studies of the contribution of the intestinal microbiota to infectious disease are discussed. We hope to provide an understanding of the human-protozoal pathogen-microbiome interaction and to speculate on how that might be leveraged for treatment.
[Mh] Termos MeSH primário: Microbioma Gastrointestinal
Interações Hospedeiro-Parasita
Parasitos/fisiologia
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Parasitos/patogenicidade
Infecções por Protozoários/terapia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE


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[PMID]:28579150
[Au] Autor:Alonso P; Engels D; Reeder J
[Ad] Endereço:Global Malaria Programme, World Health Organization, CH-1211 Geneva 27, Switzerland. Electronic address: alonsop@who.int.
[Ti] Título:Renewed push to strengthen vector control globally.
[So] Source:Lancet;389(10086):2270-2271, 2017 06 10.
[Is] ISSN:1474-547X
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Saúde Global/economia
Implementação de Plano de Saúde
Insetos Vetores
Controle de Mosquitos/métodos
[Mh] Termos MeSH secundário: Animais
Doenças Transmissíveis/epidemiologia
Doenças Transmissíveis/transmissão
Seres Humanos
Mosquiteiros Tratados com Inseticida
Infecções por Protozoários/epidemiologia
Infecções por Protozoários/prevenção & controle
Infecções por Protozoários/transmissão
Organização Mundial da Saúde
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE


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[PMID]:28506034
[Au] Autor:Xiao S; Yin P; Zhang Y; Hu S
[Ad] Endereço:School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, P. R. China.
[Ti] Título:Occurrence of and and the Relationship between Protozoa and Water Quality Indicators in Swimming Pools.
[So] Source:Korean J Parasitol;55(2):129-135, 2017 Apr.
[Is] ISSN:1738-0006
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:A total of 60 samples were collected from 35 swimming pools in Beijing, China, and the presence of and were investigated. The results showed that 16.7% and 15.0% of samples were positive for oocyst and cysts, respectively, with a mean concentration of 0.30 oocysts/10 L and 0.27 cysts/10 L. The oocysts and cysts were found to have higher rates of occurrence in August than in May. Genotyping confirmed the presence of , and assemblages A and B, all of which were associated with human infections. The predominant species/assemblages were and assemblage A. Analyses of the relationships between parasite oocysts/cysts, indicator bacteria, and physical-chemical parameters revealed that there was no correlation between 2 parasites and fecal bacterial indicators, whilst there was a significant correlation between protozoa and urea concentration, which indicates that urea concentration rather than fecal bacterial indicators might be an appropriate index for chlorine-resistant protozoa in swimming pools. This study provides useful information to improve the safety of swimming pool water and deduce the risk of protozoan infections.
[Mh] Termos MeSH primário: Cryptosporidium/isolamento & purificação
Giardia/isolamento & purificação
Piscinas
Microbiologia da Água
Qualidade da Água
[Mh] Termos MeSH secundário: China
Cryptosporidium/classificação
Cryptosporidium/genética
Genótipo
Giardia/classificação
Giardia/genética
Seres Humanos
Infecções por Protozoários/prevenção & controle
Ureia/análise
Água/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water); 8W8T17847W (Urea)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170517
[St] Status:MEDLINE
[do] DOI:10.3347/kjp.2017.55.2.129


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[PMID]:28346485
[Au] Autor:Fehlberg HF; Maciel BM; Albuquerque GR
[Ad] Endereço:Graduation program in Animal Science, Santa Cruz State University, Ilhéus (BA), Brazil.
[Ti] Título:Identification and discrimination of Toxoplasma gondii, Sarcocystis spp., Neospora spp., and Cryptosporidium spp. by righ-resolution melting analysis.
[So] Source:PLoS One;12(3):e0174168, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to standardize the high-resolution melting method for identification and discrimination of Toxoplasma gondii, Sarcocystis spp., Neospora spp., and Cryptosporidium spp. by amplification of 18S ribosomal DNA (rDNA) using a single primer pair. The analyses were performed on individual reactions (containing DNA from a single species of a protozoan), on duplex reactions (containing DNA from two species of protozoa in each reaction), and on a multiplex reaction (containing DNA of four parasites in a single reaction). The proposed method allowed us to identify and discriminate the four species by analyzing the derivative, normalized, and difference melting curves, with high reproducibility among and within the experiments, as demonstrated by low coefficients of variation (less than 2.2% and 2.0%, respectively). This is the first study where this method is used for discrimination of these four species of protozoa in a single reaction.
[Mh] Termos MeSH primário: Cryptosporidium/isolamento & purificação
DNA de Protozoário/genética
DNA Ribossômico/genética
Neospora/isolamento & purificação
Infecções por Protozoários/diagnóstico
Sarcocystis/isolamento & purificação
Toxoplasma/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Coccidiose/diagnóstico
Coccidiose/parasitologia
Criptosporidiose/diagnóstico
Criptosporidiose/parasitologia
Cryptosporidium/genética
DNA de Protozoário/isolamento & purificação
DNA Ribossômico/isolamento & purificação
Seres Humanos
Neospora/genética
Desnaturação de Ácido Nucleico
Reação em Cadeia da Polimerase
Infecções por Protozoários/parasitologia
Sarcocystis/genética
Sarcocistose/diagnóstico
Sarcocistose/parasitologia
Toxoplasma/genética
Toxoplasmose/diagnóstico
Toxoplasmose/parasitologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Protozoan); 0 (DNA, Ribosomal)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170328
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174168



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