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  1 / 9108 MEDLINE  
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[PMID]:29352974
[Au] Autor:Sassi A; Maatouk M; El Gueder D; Bzéouich IM; Abdelkefi-Ben Hatira S; Jemni-Yacoub S; Ghedira K; Chekir-Ghedira L
[Ad] Endereço:Laboratory of Cellular and Molecular Biology, Faculty of Dental Medicine, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia; Unity of Bioactive Natural Substances and Biotechnology, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir, 5000, Tunisia.
[Ti] Título:Chrysin, a natural and biologically active flavonoid suppresses tumor growth of mouse B16F10 melanoma cells: In vitro and In vivo study.
[So] Source:Chem Biol Interact;283:10-19, 2018 Mar 01.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Chrysin (5,7-dihydroxyflavone) is a natural and biologically active compound which has many biological activities as an anticancer agent. The current report is aimed at finding out whether the antitumor potential of chrysin, evidenced in vitro and in vivo, is linked or not to its effect on immunological mechanisms of melanoma-bearing mice. Chrysin-treated B16F10 cells were analyzed for their metabolic rate and apoptotic potentials. In vivo, BALB/c mice received a subcutaneous injection of B16F10 melanoma cells prior to antitumor treatments with chrysin (50 mg/kg b.w) for 14 days and 21 days. The results showed that chrysin inhibited cancer cell growth at a dose-dependent manner by inducing apoptosis and cell cycle arrest at G2/M phase. Moreover, chrysin suppressed melanoma tumor growth at an average of 60% (after 14 days of treatment) and 71% (after 21 days of treatment) compared to the tumor-bearing group. Furthermore, chrysin treatment increased the cytotoxic activity of NK, CTL and macrophages. The findings showed that chrysin antitumor action on the murine melanoma model was very promising, suggesting that chrysin could be a potentially good candidate for future use in alternative anti-melanoma treatments.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Flavonoides/toxicidade
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Flavonoides/química
Flavonoides/uso terapêutico
Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos
Células Matadoras Naturais/citologia
Células Matadoras Naturais/imunologia
Células Matadoras Naturais/metabolismo
Pontos de Checagem da Fase M do Ciclo Celular/efeitos dos fármacos
Macrófagos/citologia
Macrófagos/imunologia
Macrófagos/metabolismo
Masculino
Melanoma Experimental/tratamento farmacológico
Melanoma Experimental/metabolismo
Melanoma Experimental/patologia
Camundongos
Camundongos Endogâmicos BALB C
Óxido Nítrico/metabolismo
Linfócitos T Citotóxicos/citologia
Linfócitos T Citotóxicos/imunologia
Linfócitos T Citotóxicos/metabolismo
Transplante Homólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 31C4KY9ESH (Nitric Oxide); 3CN01F5ZJ5 (chrysin)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180122
[St] Status:MEDLINE


  2 / 9108 MEDLINE  
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[PMID]:28463694
[Au] Autor:Liu L; Yi H; He H; Pan H; Cai L; Ma Y
[Ad] Endereço:Key Lab of Health Informatics of Chinese Academy of Sciences, Guangdong Key Laboratory of Nanomedicine, Shenzhen Institutes of Advanced Technology, Chinese Academy of Science, Shenzhen 518055, PR China.
[Ti] Título:Tumor associated macrophage-targeted microRNA delivery with dual-responsive polypeptide nanovectors for anti-cancer therapy.
[So] Source:Biomaterials;134:166-179, 2017 Jul.
[Is] ISSN:1878-5905
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Repolarizing Tumor-associated macrophages (TAMs) to anti-tumor M1 macrophages with microRNA (miR) is a plausible approach for cancer treatment. However, how to achieve TAM-targeted miR delivery remains a challenge. The present study generated redox/pH dual-responsive hybrid polypeptide nanovectors, which consisted of self-crosslinked redox-responsive nanoparticles based on galactose-functionalized n-butylamine-poly(l-lysine)-b-poly(l-cysteine) polypeptides (GLC) coated with DCA-grafted sheddable PEG-PLL (sPEG) copolymers. The ex vivo study showed that sPEG shielded cationic GLC core at physiological pH but quickly shed off to re-expose GLC due to it charge reversible property. Encapsulation with sPEG/GLC nanovectors effectively facilitated macrophage-targeted miR delivery at the acidic condition but diminished miR uptake at neutral pH. Administration of miR155-loaded sPEG/GLC (sPEG/GLC/155) nanocomplexes increased miR155 expression in TAMs about 100-400 folds both in vitro and in vivo. sPEG/GLC/155 also effectively repolarized immunosuppressive TAMs to anti-tumor M1 macrophages through elevating M1 macrophage markers (IL-12, iNOS, MHC II) and suppressing M2 macrophage markers (Msr2 and Arg1) in TAMs. Moreover, the treatment of sPEG/GLC/155 significantly increased activated T lymphocytes and NK cells in tumors, which consequently led to robust tumor regression. Hence, TAM-targeted delivery of miR with redox/pH dual-responsive sPEG/GLC nanovectors could be a promising approach to re-polarize TAMs to M1 macrophages in situ and induce tumor regression.
[Mh] Termos MeSH primário: MicroRNAs/genética
Nanopartículas/química
Peptídeos/química
[Mh] Termos MeSH secundário: Animais
Eletroforese em Gel de Ágar
Feminino
Interleucina-12/metabolismo
Células Matadoras Naturais/metabolismo
Espectroscopia de Ressonância Magnética
Melanoma/metabolismo
Melanoma/terapia
Melanoma Experimental
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Nus
MicroRNAs/fisiologia
Óxido Nítrico Sintase Tipo II/metabolismo
Células RAW 264.7
Transdução de Sinais/genética
Transdução de Sinais/fisiologia
Linfócitos T/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs); 0 (Peptides); 187348-17-0 (Interleukin-12); EC 1.14.13.39 (Nitric Oxide Synthase Type II)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


  3 / 9108 MEDLINE  
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Yunes, Rosendo A
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[PMID]:28746057
[Au] Autor:Cordova CAS; Locatelli C; Winter E; Silva AH; Zanetti-Ramos BG; Jasper R; Mascarello A; Yunes RA; Nunes RJ; Creczynski-Pasa TB
[Ad] Endereço:aDepartment of Pharmaceutical Sciences bDepartment of Biochemistry cDepartment of Chemistry, Federal University of Santa Catarina dNanovetores Technology S.A., Florianópolis eUniversity of Western Santa Catarina, Videira, SC fFederal University of Tocantins, Araguaína, TO, Brazil.
[Ti] Título:Solid lipid nanoparticles improve octyl gallate antimetastatic activity and ameliorate its renal and hepatic toxic effects.
[So] Source:Anticancer Drugs;28(9):977-988, 2017 10.
[Is] ISSN:1473-5741
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Metastasis is the main cause of cancer-related death and requires the development of effective treatments with reduced toxicity and effective anticancer activity. Gallic acid derivatives have shown significant biological properties including antitumoral activity as shown in a previous study with octyl gallate (G8) in vitro. Thus, the aim of this work was to evaluate the antimetastatic effect of free and solid lipid nanoparticle-loaded G8 in mice in a lung metastasis model. Animals inoculated with melanoma cells presented metastasis in lungs, which was significantly inhibited by treatment with G8 and solid lipid nanoparticle-loaded G8, named G8-NVM. However, G8-treated mice showed an increase in several toxicological parameters, which were almost completely circumvented by G8-NVM treatment. This study supports the need for pharmacological studies on new potential medicinal plants to treat cancer and can provide new perspectives on using nanotechnology to improve biological activities while decreasing the chemotherapy toxicological effects of anticancer drugs.
[Mh] Termos MeSH primário: Doença Hepática Induzida por Substâncias e Drogas/etiologia
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle
Ácido Gálico/análogos & derivados
Nefropatias/induzido quimicamente
Nefropatias/prevenção & controle
Lipídeos/administração & dosagem
Nanopartículas/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Cercopithecus aethiops
Feminino
Ácido Gálico/administração & dosagem
Ácido Gálico/efeitos adversos
Ácido Gálico/química
Lipídeos/química
Neoplasias Pulmonares/tratamento farmacológico
Neoplasias Pulmonares/metabolismo
Neoplasias Pulmonares/secundário
Melanoma Experimental/tratamento farmacológico
Melanoma Experimental/metabolismo
Melanoma Experimental/patologia
Camundongos
Nanopartículas/química
Metástase Neoplásica
Espécies Reativas de Oxigênio/metabolismo
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Lipids); 0 (Reactive Oxygen Species); 079IIA2811 (octyl gallate); 632XD903SP (Gallic Acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1097/CAD.0000000000000539


  4 / 9108 MEDLINE  
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[PMID]:28467382
[Au] Autor:Chang NF; Chen YS; Lin YJ; Tai TH; Chen AN; Huang CH; Lin CC
[Ad] Endereço:Department of Cosmetic Science, Providence University, 200, Sec. 7, Taiwan Boulevard, Shalu Dist., Taichung 43301, Taiwan. nfchang@pu.edu.tw.
[Ti] Título:Study of Hydroquinone Mediated Cytotoxicity and Hypopigmentation Effects from UVB-Irradiated Arbutin and DeoxyArbutin.
[So] Source:Int J Mol Sci;18(5), 2017 May 03.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Arbutin (Arb) and deoxyArbutin (dA) are both effective hypopigmentation agents. However, they are glucoside derivatives of hydroquinone (HQ), which may be decayed into HQ under higher energy environments. Therefore, safety and toxicity are very important issues when considering the usage of these compounds. However, no study has verified the properties of Ultra-Violet B (UVB)-irradiated Arb and dA. In this work, we investigated the cytotoxicity and hypopigmentation effects of UVB-irradiated Arb and dA in Detroit 551 human fibroblast cells and B16-F10 mouse melanoma cells. The results showed that UVB-irradiated Arb and dA have strong cytotoxicity for the fibroblast cells, especially for dA, the caspase-3 is also activated by the treatment of UVB-irradiated dA in Detroit 551 cells. The results correlated with the produced HQ. In addition, UVB-irradiated Arb and dA suppressed the production of melanin in melanoma cells; this is due to the release of HQ that compensates for the UVB triggered Arb and dA decomposition.
[Mh] Termos MeSH primário: Arbutina/análogos & derivados
Sobrevivência Celular/efeitos dos fármacos
Hidroquinonas/toxicidade
Hipopigmentação/induzido quimicamente
[Mh] Termos MeSH secundário: Animais
Arbutina/efeitos da radiação
Arbutina/toxicidade
Caspase 3/efeitos dos fármacos
Células Cultivadas
Fibroblastos/efeitos dos fármacos
Glucosídeos
Seres Humanos
Hidroquinonas/efeitos da radiação
Melaninas/antagonistas & inibidores
Melanócitos/efeitos dos fármacos
Melanoma Experimental
Camundongos
Raios Ultravioleta
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucosides); 0 (Hydroquinones); 0 (Melanins); C5INA23HXF (Arbutin); EC 3.4.22.- (CASP3 protein, human); EC 3.4.22.- (Caspase 3); RG969BY5EN (deoxyarbutin); XV74C1N1AE (hydroquinone)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  5 / 9108 MEDLINE  
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[PMID]:29224228
[Au] Autor:Xu X; Sun Q; Mei Y; Liu Y; Zhao L
[Ad] Endereço:College of Basic Medicine and Biological Sciences, Medical Department, Soochow University, Suzhou, China.
[Ti] Título:Newcastle disease virus co-expressing interleukin 7 and interleukin 15 modified tumor cells as a vaccine for cancer immunotherapy.
[So] Source:Cancer Sci;109(2):279-288, 2018 Feb.
[Is] ISSN:1349-7006
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Interleukin 15 (IL15) and IL7 are two cytokines essential for T cell development and homeostasis. In order to improve the antitumor activity by Newcastle disease virus (NDV)-modified tumor vaccine, we generated a recombinant NDV co-expressing IL15 and IL7 (LX/IL(15+7)) through incorporation of a 2A self-processing peptide into IL15 and IL7 using reverse genetics. B16 cells infected with LX/IL(15+7) expressed both IL15 and IL7 stably. The cytotoxicity assay showed that murine melanoma cells modified with LX/IL(15+7) could significantly enhance the antitumor immune response in vitro. Then, the antitumor effects of tumor vaccine modified with recombinant virus were tested in the murine tumor models. We observed strong antitumor responses induced by LX/IL(15+7)-modified tumor cells both in prophylaxis and therapeutic models. Although the tumor-infiltrating CD4 T cells and CD8 T cells were both increased, the antitumor activity of the tumor vaccine modified with LX/IL(15+7) was dependent on CD8 T cells. Taken together, our data strongly indicated that tumor vaccine modified with NDV strain LX/IL(15+7) is a promising agent for cancer immunotherapy.
[Mh] Termos MeSH primário: Interleucina-15/metabolismo
Interleucina-7/metabolismo
Melanoma Experimental/terapia
Vírus da Doença de Newcastle/genética
[Mh] Termos MeSH secundário: Animais
Linfócitos T CD4-Positivos/metabolismo
Linfócitos T CD8-Positivos/metabolismo
Linhagem Celular Tumoral
Proliferação Celular
Sobrevivência Celular
Vetores Genéticos/administração & dosagem
Imunoterapia
Interleucina-15/genética
Interleucina-7/genética
Melanoma Experimental/imunologia
Camundongos
Vírus da Doença de Newcastle/crescimento & desenvolvimento
Genética Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-15); 0 (Interleukin-7)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171211
[St] Status:MEDLINE
[do] DOI:10.1111/cas.13468


  6 / 9108 MEDLINE  
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[PMID]:29320562
[Au] Autor:Jarosz-Biej M; Kaminska N; Matuszczak S; Cichon T; Pamula-Pilat J; Czapla J; Smolarczyk R; Skwarzynska D; Kulik K; Szala S
[Ad] Endereço:Center for Translational Research and Molecular Biology of Cancer, Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Gliwice, Poland.
[Ti] Título:M1-like macrophages change tumor blood vessels and microenvironment in murine melanoma.
[So] Source:PLoS One;13(1):e0191012, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tumor-associated macrophages (TAMs) play a significant role in at least two key processes underlying neoplastic progression: angiogenesis and immune surveillance. TAMs phenotypic changes play important role in tumor vessel abnormalization/ normalization. M2-like TAMs stimulate immunosuppression and formation of defective tumor blood vessels leading to tumor progression. In contrast M1-like TAMs trigger immune response and normalize irregular tumor vascular network which should sensitize cancer cells to chemo- and radiotherapy and lead to tumor growth regression. Here, we demonstrated that combination of endoglin-based DNA vaccine with interleukin 12 repolarizes TAMs from tumor growth-promoting M2-like phenotype to tumor growth-inhibiting M1-like phenotype. Combined therapy enhances tumor infiltration by CD4+, CD8+ lymphocytes and NK cells. Depletion of TAMs as well as CD8+ lymphocytes and NK cells, but not CD4+ lymphocytes, reduces the effect of combined therapy. Furthermore, combined therapy improves tumor vessel maturation, perfusion and reduces hypoxia. It caused that suboptimal doses of doxorubicin reduced the growth of tumors in mice treated with combined therapy. To summarize, combination of antiangiogenic drug and immunostimulatory agent repolarizes TAMs phenotype from M2-like (pro-tumor) into M1-like (anti-tumor) which affects the structure of tumor blood vessels, improves the effect of chemotherapy and leads to tumor growth regression.
[Mh] Termos MeSH primário: Interleucina-12/administração & dosagem
Macrófagos/fisiologia
Melanoma Experimental/irrigação sanguínea
Melanoma Experimental/imunologia
Neovascularização Patológica/patologia
Microambiente Tumoral/imunologia
[Mh] Termos MeSH secundário: Inibidores da Angiogênese/administração & dosagem
Animais
Antibióticos Antineoplásicos/farmacologia
Linfócitos T CD4-Positivos/efeitos dos fármacos
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD8-Positivos/efeitos dos fármacos
Linfócitos T CD8-Positivos/imunologia
Proliferação Celular/efeitos dos fármacos
Doxorrubicina/farmacologia
Feminino
Células Matadoras Naturais/efeitos dos fármacos
Células Matadoras Naturais/imunologia
Macrófagos/efeitos dos fármacos
Melanoma Experimental/tratamento farmacológico
Melanoma Experimental/patologia
Camundongos
Camundongos Endogâmicos C57BL
Neovascularização Patológica/tratamento farmacológico
Neovascularização Patológica/imunologia
Células Tumorais Cultivadas
Vacinas de DNA/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Angiogenesis Inhibitors); 0 (Antibiotics, Antineoplastic); 0 (Vaccines, DNA); 187348-17-0 (Interleukin-12); 80168379AG (Doxorubicin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191012


  7 / 9108 MEDLINE  
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[PMID]:29313327
[Au] Autor:Chai WM; Huang Q; Lin MZ; Ou-Yang C; Huang WY; Wang YX; Xu KL; Feng HL
[Ad] Endereço:College of Life Science and Key Laboratory of Small Functional Organic Molecule, Ministry of Education, Jiangxi Normal University , Nanchang, Jiangxi 330022, People's Republic of China.
[Ti] Título:Condensed Tannins from Longan Bark as Inhibitor of Tyrosinase: Structure, Activity, and Mechanism.
[So] Source:J Agric Food Chem;66(4):908-917, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, the content, structure, antityrosinase activity, and mechanism of longan bark condensed tannins were evaluated. The findings obtained from mass spectrometry demonstrated that longan bark condensed tannins were mixtures of procyanidins, propelargonidins, prodelphinidins, and their acyl derivatives (galloyl and p-hydroxybenzoate). The enzyme analysis indicated that these mixtures were efficient, reversible, and mixed (competitive is dominant) inhibitor of tyrosinase. What's more, the mixtures showed good inhibitions on proliferation, intracellular enzyme activity and melanogenesis of mouse melanoma cells (B ). From molecular docking, the results showed the interactions between inhibitors and tyrosinase were driven by hydrogen bond, electrostatic, and hydrophobic interactions. In addition, high levels of total phenolic and extractable condensed tannins suggested that longan bark might be a good source of tyrosinase inhibitor. This study would offer theoretical basis for the development of longan bark condensed tannins as novel food preservatives and medicines of skin diseases.
[Mh] Termos MeSH primário: Inibidores Enzimáticos/farmacologia
Monofenol Mono-Oxigenase/antagonistas & inibidores
Casca de Planta/química
Sapindaceae/química
Taninos/química
Taninos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antocianinas/farmacologia
Biflavonoides/farmacologia
Catequina/farmacologia
Proliferação Celular/efeitos dos fármacos
Ligações de Hidrogênio
Interações Hidrofóbicas e Hidrofílicas
Espectrometria de Massas
Melaninas/análise
Melaninas/antagonistas & inibidores
Melaninas/biossíntese
Melanoma Experimental
Camundongos
Modelos Moleculares
Simulação de Acoplamento Molecular
Oxirredutases
Parabenos/farmacologia
Proantocianidinas/farmacologia
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Eletricidade Estática
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Biflavonoids); 0 (Enzyme Inhibitors); 0 (Melanins); 0 (Parabens); 0 (Proanthocyanidins); 0 (Tannins); 4852-22-6 (procyanidin); 8R1V1STN48 (Catechin); EC 1.- (Oxidoreductases); EC 1.14.18.- (monophenolase); EC 1.14.18.1 (Monophenol Monooxygenase); EM6MD4AEHE (delphinidin); JG8Z55Y12H (4-hydroxybenzoic acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05481


  8 / 9108 MEDLINE  
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[PMID]:27774782
[Au] Autor:Yahav G; Barnoy E; Roth N; Turgeman L; Fixler D
[Ad] Endereço:Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Bar Ilan University, 5290002, Ramat Gan, Israel.
[Ti] Título:Reference-independent wide field fluorescence lifetime measurements using Frequency-Domain (FD) technique based on phase and amplitude crossing point.
[So] Source:J Biophotonics;10(9):1198-1207, 2017 Sep.
[Is] ISSN:1864-0648
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Fluorescence lifetime imaging microscopy (FLIM) is an essential tool in many scientific fields such as biology and medicine thanks to the known advantages of the fluorescence lifetime (FLT) over the classical fluorescence intensity (FI). However, the frequency domain (FD) FLIM technique suffers from its strong dependence on the reference and its compliance to the sample. In this paper, we suggest a new way to calculate the FLT by using the crossing point (CRPO) between the modulation and phase FLTs measured over several light emitting diode (LED) DC currents values instead of either method alone. This new technique was validated by measuring homogeneous substances with known FLT, where the CRPO appears to be the optimal measuring point. Furthermore, the CRPO method was applied in heterogeneous samples. It was found that the CRPO in known mixed solutions is the weighted average of the used solutions. While measuring B16 and lymphocyte cells, the CRPO of the DAPI compound in single FLT regions was measured at 3.5 ± 0.06 ns and at 2.83 ± 0.07 ns, respectively, both of which match previous reports and multi-frequency analyses. This paper suggests the CRPO as a new method to extract the FLT in problematic cases such as high MCP gains and heterogeneous environments. In traditional FD FLIM measurements, the variation in phase angle and modulation are measured. By measuring over varying DC currents, another variation is detected in the FLT determined through the phase and modulation methods, with the CRPO indicating the true FLT.
[Mh] Termos MeSH primário: Microscopia de Fluorescência
Imagem Óptica
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Linfócitos
Melanoma Experimental
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1002/jbio.201600220


  9 / 9108 MEDLINE  
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[PMID]:27776244
[Au] Autor:Goswami KK; Sarkar M; Ghosh S; Saha A; Ghosh T; Guha I; Barik S; Banerjee S; Roy S; Bose A; Dasgupta P; Baral R
[Ad] Endereço:Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, 37, S. P. Mukherjee Road, Kolkata 700026, India.
[Ti] Título:Neem leaf glycoprotein regulates function of tumor associated M2 macrophages in hypoxic tumor core: Critical role of IL-10/STAT3 signaling.
[So] Source:Mol Immunol;80:1-10, 2016 12.
[Is] ISSN:1872-9142
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Heterogeneous tumor microenvironment (TME), broadly divided into tumor core and peripheral sub-microenvironments, differentially polarize normal macrophages into a different form known as tumor associated M2 macrophages (M2TAMs) to promote tumor growth. In view of the extensive immune-editing role of NLGP, here, we have observed that NLGP is effective to convert M2TAMs (CD11b F4/80 ) to M1 (CD11b F4/80 ) more prominently in tumor core, along with downregulation of other M2 associated markers, like, ManR, Ym1, Fizz1. High IL-10:IL-12 ratio at tumor core was downregulated in NLGP treated melanoma bearing mice. Decrease in IL-10 by NLGP is again associated with the decrease in hypoxia, as indicated by prominent downregulation of HIF1α and VEGF, particularly at tumor core. Macrophages exposed to hypoxic tumor core lysates in vitro exhibited high IL-10, HIF1α and VEGF expression that was significantly downregulated by NLGP. Further evidences suggest M2TAM to M1 conversion by NLGP is associated with STAT3-regulated IL-10 dependent pathway without affecting the IL-4 dependent one. Such TAM modulatory functions of NLGP might help in the restriction of melanoma growth by increasing the proportion of M1 TAMs in tumor core that helps in prevention of tumor relapse and dissemination of the tumor mass.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Macrófagos/imunologia
Melanoma Experimental/imunologia
Proteínas de Plantas/farmacologia
Transdução de Sinais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Azadirachta
Western Blotting
Hipóxia Celular/efeitos dos fármacos
Citometria de Fluxo
Glicoproteínas/farmacologia
Imuno-Histoquímica
Interleucina-10/imunologia
Macrófagos/efeitos dos fármacos
Melanoma Experimental/patologia
Camundongos
Camundongos Endogâmicos C57BL
Extratos Vegetais/farmacologia
Folhas de Planta
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Fator de Transcrição STAT3/imunologia
Transdução de Sinais/imunologia
Microambiente Tumoral/efeitos dos fármacos
Microambiente Tumoral/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Glycoproteins); 0 (IL10 protein, mouse); 0 (Plant Extracts); 0 (Plant Proteins); 0 (STAT3 Transcription Factor); 0 (Stat3 protein, mouse); 130068-27-8 (Interleukin-10)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:29240845
[Au] Autor:Reimer M; Denby E; Zustiak SP; Schober JM
[Ad] Endereço:Department of Pharmaceutical Sciences, Southern Illinois University Edwardsville, Edwardsville, Illinois, United States of America.
[Ti] Título:Ras GAP-related and C-terminal domain-dependent localization and tumorigenic activities of IQGAP1 in melanoma cells.
[So] Source:PLoS One;12(12):e0189589, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:IQGAP1 interacts with a number of binding partners through a calponin homology domain (CHD), a WW motif, IQ repeats, a Ras GAP-related domain (GRD), and a conserved C-terminal (CT) domain. Among various biological and cellular functions, IQGAP1 is known to play a role in actin cytoskeleton dynamics during membrane ruffling and lamellipodium protrusion. In addition, phosphorylation near the CT domain is thought to control IQGAP1 activity through regulation of intramolecular interaction. In a previous study, we discovered that IQGAP1 preferentially localizes to retracting areas in B16F10 mouse melanoma cells, not areas of membrane ruffling and lamellipodium protrusion. Nothing is known of the domains needed for retraction localization and very little is known of IQGAP1 function in the actin cytoskeleton of melanoma cells. Thus, we examined localization of IQGAP1 mutants to retracting areas, and characterized knock down phenotypes on tissue culture plastic and physiologic-stiffness hydrogels. Localization of IQGAP1 mutants (S1441E/S1443D, S1441A/S1443A, ΔCHD, ΔGRD or ΔCT) to retracting and protruding cell edges were measured. In retracting areas there was a decrease in S1441A/S1443A, ΔGRD and ΔCT localization, a minor decrease in ΔCHD localization, and normal localization of the S1441E/S1443D mutant. In areas of cell protrusion just behind the lamellipodium leading edge, we surprisingly observed both ΔGRD and ΔCT localization, and increased number of microtubules. IQGAP1 knock down caused loss of cell polarity on laminin-coated glass, decreased proliferation on tissue culture polystyrene, and abnormal spheroid growth on laminin-coated hydrogels. We propose that the GRD and CT domains regulate IQGAP1 localization to retracting actin networks to promote a tumorigenic role in melanoma cells.
[Mh] Termos MeSH primário: Melanoma Experimental/metabolismo
Proteínas Ativadoras de ras GTPase/metabolismo
[Mh] Termos MeSH secundário: Citoesqueleto de Actina/metabolismo
Animais
Carcinogênese
Linhagem Celular Tumoral
Técnicas de Silenciamento de Genes
Hidrogéis
Melanoma Experimental/patologia
Camundongos
Microtúbulos/metabolismo
Mutação
Pseudópodes/metabolismo
Proteínas Ativadoras de ras GTPase/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrogels); 0 (IQ motif containing GTPase activating protein 1); 0 (ras GTPase-Activating Proteins)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189589



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