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[PMID]:28461165
[Au] Autor:Ahmed FRS; Amin R; Hasan I; Asaduzzaman AKM; Kabir SR
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Faculty of Science, University of Rajshahi, Rajshahi 6205, Bangladesh.
[Ti] Título:Antitumor properties of a methyl-ß-d-galactopyranoside specific lectin from Kaempferia rotunda against Ehrlich ascites carcinoma cells.
[So] Source:Int J Biol Macromol;102:952-959, 2017 Sep.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A lectin was isolated from the tuberous rhizome of Keampferia rotunda by using different chromatographic methods with the molecular weight of 21±1kDa. The lectin contained highest percentage of leucine and lowest percentage of tryptophan residues as determined by LC-MS. The lectin agglutinated mice and human erythrocytes and the hemagglutination activity was inhibited by Methyl-ß-d-galactopyranoside. The lectin did not lose its activity in the presence of urea but the activity abolished completely when treated with EDTA. The lectin exhibited its activity at the pH ranging from 6.0 to 9.0 and in a temperature range of 30-80°C. Antiproliferative activity was studied against Ehrlich ascites carcinoma (EAC) and U87 cell lines. No inhibitory effect was observed against U87 cell line whereas 43.7% cell growth inhibition was observed in vitro against EAC cells at 160µg/ml. The lectin was injected (i.p.) in EAC bearing Swiss albino mice at the doses of 3.0 and 6.0mg/kg/day for five consecutive days and 41 and 59% of EAC cell growth inhibition was observed, respectively. The cell growth inhibition was due to the induction of apoptosis in the EAC cells which was confirmed by cell morphological study, caspase-3 inhibitor and apoptosis-related gene expression.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Carcinoma de Ehrlich/patologia
Galactose/metabolismo
Lectinas de Plantas/farmacologia
Zingiberaceae/química
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/química
Antineoplásicos/isolamento & purificação
Antineoplásicos/metabolismo
Apoptose/efeitos dos fármacos
Apoptose/genética
Caspases/metabolismo
Bovinos
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Hemaglutinação/efeitos dos fármacos
Seres Humanos
Concentração de Íons de Hidrogênio
Camundongos
Peso Molecular
Lectinas de Plantas/química
Lectinas de Plantas/isolamento & purificação
Lectinas de Plantas/metabolismo
Especificidade por Substrato
Temperatura Ambiente
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Plant Lectins); EC 3.4.22.- (Caspases); X2RN3Q8DNE (Galactose)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE


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[PMID]:29318287
[Au] Autor:Abu-Melha S
[Ti] Título:Synthesis and Biological Evaluation of Some Novel 1,8-Naphthyridine Derivatives.
[So] Source:Acta Chim Slov;64(4):919-930, 2017 Dec.
[Is] ISSN:1318-0207
[Cp] País de publicação:Slovenia
[La] Idioma:eng
[Ab] Resumo:A series of substituted 1,8-naphthyridine derivatives was synthesized to be used as cytotoxic and antioxidant agents by applying 1,4-dihydro-4-oxo-1,8-naphthyridine-3-carbohydrazide (1) as the starting material. Compound 1 was reacted with different reagents to afford the corresponding 3-heterarylcarbonyl-1,8-naphthyridine derivatives 3-19 which were tested for their in vitro cytotoxicity against Ehrlich Ascites Carcinoma, and antioxidant activity. Compound 15 showed the best cytotoxicity and antioxidant activity.
[Mh] Termos MeSH primário: Antineoplásicos/síntese química
Antioxidantes/síntese química
Naftiridinas/síntese química
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/farmacologia
Antioxidantes/farmacologia
Carcinoma de Ehrlich/tratamento farmacológico
Naftiridinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Antioxidants); 0 (Naphthyridines)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180306
[Lr] Data última revisão:
180306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180111
[St] Status:MEDLINE


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[PMID]:29215829
[Au] Autor:Kalish SV; Lyamina SV; Raetskaya AA; Malyshev II
[Ti] Título:[Reprogrammed M1 macrophages with inhibited STAT3, STAT6 and/or SMAD3 extends lifespan of mice with experimental carcinoma].
[So] Source:Patol Fiziol Eksp Ter;61(2):4-9, 2017 Apr-Jun.
[Is] ISSN:0031-2991
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Objective. Reprogramming of M1 macrophage phenotype with inhibited M2 phenotype transcription factors, such as STAT3, STAT6 and SMAD and assess their impact on the development of Ehrlich carcinoma (EC) in vitro and in vivo. Methods. Tumor growth in vitro was initiated by addition of EC cells in RPMI-1640 culture medium and in vivo by intraperitoneal of EC cell injection into mice. Results. It was found that M1-STAT3/6- SMAD3 macrophages have a pronounced anti-tumor effect in vitro, and in vivo, which was greater than anti-tumor effects of M1, M1-STAT 3/6, M1-SMAD3 macrophages and cisplatin. Conclusion. M1 macrophages with inhibited STAT3, STAT6 and/or SMAD3 effectively restrict tumor growth. The findings justify the development of new anti-tumor cell therapy technology.
[Mh] Termos MeSH primário: Carcinoma de Ehrlich
Reprogramação Celular/imunologia
Macrófagos/imunologia
Fator de Transcrição STAT3/imunologia
Fator de Transcrição STAT6/imunologia
Proteína Smad3/imunologia
[Mh] Termos MeSH secundário: Animais
Carcinoma de Ehrlich/imunologia
Carcinoma de Ehrlich/patologia
Carcinoma de Ehrlich/terapia
Camundongos
Camundongos Endogâmicos BALB C
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (STAT3 Transcription Factor); 0 (STAT6 Transcription Factor); 0 (Smad3 Protein); 0 (Smad3 protein, mouse); 0 (Stat3 protein, mouse); 0 (Stat6 protein, mouse)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171221
[Lr] Data última revisão:
171221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171208
[St] Status:MEDLINE


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[PMID]:29022496
[Au] Autor:Medhat AM; Azab KS; Said MM; El Fatih NM; El Bakary NM
[Ad] Endereço:1 Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
[Ti] Título:Antitumor and radiosensitizing synergistic effects of apigenin and cryptotanshinone against solid Ehrlich carcinoma in female mice.
[So] Source:Tumour Biol;39(10):1010428317728480, 2017 Oct.
[Is] ISSN:1423-0380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Considerable attention has been paid to the introduction of novel naturally occurring plant-derived radiosensitizer compounds in order to augment the radiation efficacy and improve the treatment outcome of different tumors. This study was therefore undertaken to evaluate the antitumor, antiangiogeneic, and synergistic radiosensitizing effects of apigenin, a dietary flavonoid, and/or cryptotanshinone, a terpenoid isolated from the roots of Salvia miltiorrhiza, against the growth of solid Ehrlich carcinoma in female mice. Apigenin (50 mg/kg body weight) and/or cryptotanshinone (40 mg/kg body weight) was intraperitoneally (i.p.) injected into non-irradiated or γ-irradiated (6.5 Gy whole-body γ-irradiation) solid Ehrlich carcinoma-bearing mice for 30 consecutive days. Investigations included molecular targets involved in proliferation, inflammation, angiogenesis, and tumor invasiveness. Treatment with apigenin and/or cryptotanshinone significantly suppressed the growth of solid Ehrlich carcinoma tumors and demonstrated a synergistic radiosensitizing efficacy together with γ-irradiation. These effects were achieved through downregulating the expression of angiogenic and lymphangiogenic regulators, including signal transducer and activator of transcription 3, vascular endothelial growth factor C, and tumor necrosis factor alpha, suppressing matrix metalloproteinase-2 and -9 activities, which play a key role in tumor invasion and metastasis, and enhancing apoptosis via inducing cleaved caspase-3 and granzyme B levels. Histological findings of solid Ehrlich carcinoma tumors verified the recorded data. In conclusion, a synergistic radiosensitizing efficacy for apigenin and cryptotanshinone was demonstrated against Ehrlich carcinoma in the current in vivo murine model, representing therefore a potential therapeutic strategy for increasing the radiation response of solid tumors.
[Mh] Termos MeSH primário: Apigenina/administração & dosagem
Carcinoma de Ehrlich/tratamento farmacológico
Carcinoma de Ehrlich/radioterapia
Radiossensibilizantes/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Apoptose/efeitos da radiação
Carcinoma de Ehrlich/patologia
Proliferação Celular/efeitos dos fármacos
Proliferação Celular/efeitos da radiação
Modelos Animais de Doenças
Feminino
Raios gama
Seres Humanos
Camundongos
Fenantrenos/administração & dosagem
Irradiação Corporal Total
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenanthrenes); 0 (Radiation-Sensitizing Agents); 5E9SXT166N (cryptotanshinone); 7V515PI7F6 (Apigenin)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171019
[Lr] Data última revisão:
171019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171013
[St] Status:MEDLINE
[do] DOI:10.1177/1010428317728480


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[PMID]:29019283
[Au] Autor:Mello-Andrade F; da Costa WL; Pires WC; Pereira FC; Cardoso CG; Lino-Junior RS; Irusta VRC; Carneiro CC; de Melo-Reis PR; Castro CH; Almeida MAP; Batista AA; Silveira-Lacerda EP
[Ad] Endereço:1 Laboratório de Genética Molecular e Citogenética, Departamento de Genética, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, Brazil.
[Ti] Título:Antitumor effectiveness and mechanism of action of Ru(II)/amino acid/diphosphine complexes in the peritoneal carcinomatosis progression.
[So] Source:Tumour Biol;39(10):1010428317695933, 2017 Oct.
[Is] ISSN:1423-0380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Peritoneal carcinomatosis is considered as a potentially lethal clinical condition, and the therapeutic options are limited. The antitumor effectiveness of the [Ru(l-Met)(bipy)(dppb)]PF (1) and the [Ru(l-Trp)(bipy)(dppb)]PF (2) complexes were evaluated in the peritoneal carcinomatosis model, Ehrlich ascites carcinoma-bearing Swiss mice. This is the first study that evaluated the effect of Ru(II)/amino acid complexes for antitumor activity in vivo. Complexes 1 and 2 (2 and 6 mg kg ) showed tumor growth inhibition ranging from moderate to high. The mean survival time of animal groups treated with complexes 1 and 2 was higher than in the negative and vehicle control groups. The induction of Ehrlich ascites carcinoma in mice led to alterations in hematological and biochemical parameters, and not the treatment with complexes 1 and 2. The treatment of Ehrlich ascites carcinoma-bearing mice with complexes 1 and 2 increased the number of Annexin V positive cells and cleaved caspase-3 levels and induced changes in the cell morphology and in the cell cycle phases by induction of sub-G1 and G0/G1 cell cycle arrest. In addition, these complexes reduce angiogenesis induced by Ehrlich ascites carcinoma cells in chick embryo chorioallantoic membrane model. The treatment with the LAT1 inhibitor decreased the sensitivity of the Ehrlich ascites carcinoma cells to complexes 1 and 2 in vitro-which suggests that the LAT1 could be related to the mechanism of action of amino acid/ruthenium(II) complexes, consequently decreasing the glucose uptake. Therefore, these complexes could be used to reduce tumor growth and increase mean survival time with less toxicity than cisplatin. Besides, these complexes induce apoptosis by combination of different mechanism of action.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Carcinoma de Ehrlich/patologia
Neoplasias Peritoneais/patologia
Compostos de Rutênio/farmacologia
[Mh] Termos MeSH secundário: Aminoácidos/farmacologia
Animais
Western Blotting
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 0 (Antineoplastic Agents); 0 (Ruthenium Compounds)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1177/1010428317695933


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[PMID]:28864893
[Au] Autor:Inzhevatkin EV; Savchenko AA
[Ad] Endereço:Federal Research Center Krasnoyarsk Scientific Center of the Siberian Branch of the RAS, Akademgorodok 50, Krasnoyarsk, 660036, Russia. inscience@mail.ru.
[Ti] Título:The metabolic changes in tumor-associated macrophages during cancer grow in mice with Ehrlich ascites carcinoma.
[So] Source:Dokl Biochem Biophys;475(1):277-279, 2017 Jul.
[Is] ISSN:1608-3091
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:In this work, we investigated the activity of the key NAD(P)-dependent dehydrogenases associated with macrophage tumors in mice with Ehrlich ascites carcinoma. It was shown that cancer grow is associated with the development of conditions in macrophages leading to a decrease in the substrate flow intensity in the tricarboxylic acid cycle, deceleration of oxidative deamination of L-glutamate, NADP regeneration, and a decrease in the antioxidant defense efficiency. There results are consistent with our recent concept on the nonspecific metabolic reaction of cells to extreme exposures.
[Mh] Termos MeSH primário: Carcinoma de Ehrlich/metabolismo
Carcinoma de Ehrlich/patologia
Macrófagos/metabolismo
[Mh] Termos MeSH secundário: Animais
Proliferação Celular
Masculino
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170903
[St] Status:MEDLINE
[do] DOI:10.1134/S1607672917040081


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[PMID]:28756149
[Au] Autor:Havelek R; Muthna D; Tomsik P; Kralovec K; Seifrtova M; Cahlikova L; Hostalkova A; Safratova M; Perwein M; Cermakova E; Rezacova M
[Ad] Endereço:Department of Medical Biochemistry, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, Hradec Kralove 500 03, Czech Republic. Electronic address: havelekr@lfhk.cuni.cz.
[Ti] Título:Anticancer potential of Amaryllidaceae alkaloids evaluated by screening with a panel of human cells, real-time cellular analysis and Ehrlich tumor-bearing mice.
[So] Source:Chem Biol Interact;275:121-132, 2017 Sep 25.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:In this study, twenty-two Amaryllidaceae alkaloids were screened for their anticancer potential. All isolates were evaluated for antiproliferative activities on a panel of 17 human cell types of different tissue origin using WST-1 assay. In addition, we determined the antiproliferative effect with a real-time cell analysis xCELLigence system. Thereafter, to evaluate the barely known in vivo anticancer potential of the most potent molecule haemanthamine, a preliminary study was performed using an Ehrlich tumor-bearing mice model. The results showed that haemanthamine, lycorine and haemanthidine exerted the highest antiproliferative activity. The mean growth percent (GP) value after a single-dose 10 µM treatment was for haemanthamine 21%, for lycorine 21% and for haemanthidine 27% that of untreated control cells (100%). Furthermore, haemanthamine, lycorine and haemanthidine exhibited significant cytotoxicities against all the tested cell lines with individual IC values in the micromolar range. Dynamic real-time measures of impedance by xCELLigence indicated that these three compounds suppress cell proliferation after 10 h of treatment at a concentration of 10 µM or higher. Regrettably, in a follow-up in vivo antitumor activity study, haemanthamine showed no statistically significant reduction in the tumor size with no prolongation of survival time of Ehrlich tumor-bearing mice. Taken together, these results provide a new clue and guidance for exploiting Amaryllidaceae alkaloids as anticancer agents.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Amaryllidaceae/química
Apoptose/efeitos dos fármacos
[Mh] Termos MeSH secundário: Alcaloides/química
Alcaloides/uso terapêutico
Amaryllidaceae/metabolismo
Animais
Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/farmacologia
Antineoplásicos Fitogênicos/uso terapêutico
Carcinoma de Ehrlich/tratamento farmacológico
Carcinoma de Ehrlich/mortalidade
Carcinoma de Ehrlich/patologia
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Ensaios de Seleção de Medicamentos Antitumorais
Feminino
Seres Humanos
Estimativa de Kaplan-Meier
Camundongos
Transplante Heterólogo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Antineoplastic Agents, Phytogenic)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170731
[St] Status:MEDLINE


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[PMID]:28673306
[Au] Autor:Rolim TL; Meireles DRP; Batista TM; de Sousa TKG; Mangueira VM; de Abrantes RA; Pita JCLR; Xavier AL; Costa VCO; Batista LM; da Silva MS; Sobral MV
[Ad] Endereço:Programa de Pós-graduação em Produtos Naturais e Sintéticos Bioativos, Centro de Ciências da Saúde, Universidade Federal da Paraíba, João Pessoa, Paraíba, 58051-970, Brazil.
[Ti] Título:Toxicity and antitumor potential of Mesosphaerum sidifolium (Lamiaceae) oil and fenchone, its major component.
[So] Source:BMC Complement Altern Med;17(1):347, 2017 Jul 03.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The essential oil from Mesosphaerum sidifolium (L'Hérit.) Harley & J.F.B.Pastore (syn. Hyptis umbrosa), Lamiaceae (EOM), and its major component, have been tested for toxicity and antitumor activity. METHODS: EOM was obtained from aerial parts of M. sidifolium subjected to hydro distillation, and gas chromatography-mass spectrometry was used to characterize the EOM chemical composition. The toxicity was evaluated using haemolysis assay, and acute toxicity and micronucleus tests. Ehrlich ascites carcinoma model was used to evaluate the in vivo antitumor activity and toxicity of EOM (50, 100 and 150 mg/kg), and fenchone (30 and 60 mg/kg) after 9 d of treatment. RESULTS: The EOM major components were fenchone (24.8%), cubebol (6.9%), limonene (5.4%), spathulenol (4.5%), ß-caryophyllene (4.6%) and α-cadinol (4.7%). The HC50 (concentration producing 50% haemolysis) was 494.9 µg/mL for EOM and higher than 3000 µg/mL for fenchone. The LD50 for EOM was approximately 500 mg/kg in mice. The essential oil induced increase of micronucleated erythrocytes only at 300 mg/kg, suggesting moderate genotoxicity. EOM (100 or 150 mg/kg) and fenchone (60 mg/kg) reduced all analyzed parameters (tumor volume and mass, and total viable cancer cells). Survival also increased for the treated animals with EOM and fenchone. For EOM 150 mg/kg and 5-FU treatment, most cells were arrested in the G0/G1 phase, whereas for fenchone, cells arrested in the S phase, which represents a blockage in cell cycle progression. Regarding the toxicological evaluation, EOM induced weight loss, but did not induce hematological, biochemical or histological (liver and kidneys) toxicity. Fenchone induced decrease of AST and ALT, suggesting liver damage. CONCLUSIONS: The data showed EOM caused in vivo cell growth inhibition on Ehrlich ascites carcinoma model by inducing cell cycle arrest, without major changes in the toxicity parameters evaluated. In addition, this activity was associated with the presence of fenchone, its major component.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/administração & dosagem
Carcinoma de Ehrlich/tratamento farmacológico
Lamiaceae/química
Norbornanos/administração & dosagem
Óleos Voláteis/administração & dosagem
Óleos Vegetais/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/toxicidade
Carcinoma de Ehrlich/fisiopatologia
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Feminino
Seres Humanos
Camundongos
Norbornanos/química
Norbornanos/toxicidade
Óleos Voláteis/química
Óleos Voláteis/toxicidade
Óleos Vegetais/química
Óleos Vegetais/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Norbornanes); 0 (Oils, Volatile); 0 (Plant Oils); 1195-79-5 (fenchone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170705
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1779-z


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[PMID]:28424024
[Au] Autor:Murali M; Mahendra C; Hema P; Rajashekar N; Nataraju A; Sudarshana MS; Amruthesh KN
[Ad] Endereço:a Applied Plant Pathology Laboratory, Department of Studies in Botany , University of Mysore , Mysuru , India.
[Ti] Título:Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta: a medicinal plant.
[So] Source:Pharm Biol;55(1):1623-1630, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Sida acuta Burm.f. (Malvaceae) extracts are reported to have applications against malaria, diuretic, antipyretic, nervous and urinary diseases. No fungal endophytes of S. acuta are reported. OBJECTIVE: Isolation, identification and evaluation of antibacterial, antioxidant, anticancer and haemolytic potential of fungal endophytes from the ethnomedcinal plant S. acuta. MATERIALS AND METHODS: Sida acuta stem segments were placed on PDA medium to isolate endophytic fungi. The fungus was identified by genomic DNA analysis and phylogenetic tree was constructed using ITS sequences (GenBank) to confirm species. The antibacterial efficacy of Aspergillus sulphureus MME12 ethyl acetate extract was tested against Gram-positive and Gram-negative pathogenic bacteria. DPPH free radical scavenging activity, anticancer and DNA fragmentation against EAC cells, and direct haemolytic activity (100-500 µg/mL) using human erythrocytes were determined. RESULTS AND DISCUSSION: The ethyl acetate extract of A. sulphureus (Fresen.) Wehmer (Trichocomaceae) demonstrated significant antibacterial potential against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi compared to streptomycin. MIC against test pathogens was in the range of 15.6-62.5 µg/mL. The antioxidant results revealed significant RSA from 12.43% to 62.02% (IC = 350.4 µg/mL, p ≤ 0.05). MME12 offered considerable inhibition of EAC proliferation (23% to 84%, IC = 216.7 µg/mL, p ≤ 0.05) supported by DNA fragmentation studies. The extract also offered insignificant haemolysis (5.6%) compared to Triton X-100. CONCLUSIONS: A single endophytic fungus, A. sulphureus MME12 was isolated and identified using molecular profiling. The above-mentioned findings support the pharmacological application of A. sulphureus MME12 extract and demand for purification of the active principle(s).
[Mh] Termos MeSH primário: Aspergillus/isolamento & purificação
Endófitos/isolamento & purificação
Malvaceae/microbiologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Antibacterianos/administração & dosagem
Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
Antineoplásicos/administração & dosagem
Antineoplásicos/isolamento & purificação
Antineoplásicos/farmacologia
Antioxidantes/administração & dosagem
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Aspergillus/metabolismo
Carcinoma de Ehrlich/tratamento farmacológico
Carcinoma de Ehrlich/patologia
Fragmentação do DNA/efeitos dos fármacos
Relação Dose-Resposta a Droga
Endófitos/metabolismo
Eritrócitos/efeitos dos fármacos
Eritrócitos/metabolismo
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Positivas/efeitos dos fármacos
Hemólise/efeitos dos fármacos
Seres Humanos
Testes de Sensibilidade Microbiana
Extratos Vegetais/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antineoplastic Agents); 0 (Antioxidants); 0 (Plant Extracts)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.1080/13880209.2017.1315435


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[PMID]:28367666
[Au] Autor:M HR; Ghosh D; Banerjee R; Salimath BP
[Ad] Endereço:a Department of Studies in Biotechnology , Molecular Oncology Lab, University of Mysore , Mysore , India.
[Ti] Título:Suppression of VEGF-induced angiogenesis and tumor growth by Eugenia jambolana, Musa paradisiaca, and Coccinia indica extracts.
[So] Source:Pharm Biol;55(1):1489-1499, 2017 Dec.
[Is] ISSN:1744-5116
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:CONTEXT: Abnormal angiogenesis and evasion of apoptosis are hallmarks of cancer. Accordingly, anti-angiogenic and pro-apoptotic therapies are effective strategies for cancer treatment. Medicinal plants, namely, Eugenia jambolana Lam. (Myrtaceae), Musa paradisiaca L. (Musaceae), and Coccinia indica Wight & Arn. (Cucurbitaceae), have not been greatly investigated for their anticancer potential. OBJECTIVE: We investigated the anti-angiogenic and pro-apoptotic efficacy of ethyl acetate (EA) and n-butanol (NB) extracts of E. jambolana (seeds), EA extracts of M. paradisiaca (roots) and C. indica (leaves) with respect to mammary neoplasia. MATERIALS AND METHODS: Effect of extracts (2-200 µg/mL) on cytotoxicity and MCF-7, MDA-MB-231 and endothelial cell (EC) proliferation and in vitro angiogenesis were evaluated by MTT, [H]thymidine uptake and EC tube formation assays, respectively. In vivo tumour proliferation, VEGF secretion and angiogenesis were assessed using the Ehrlich ascites tumour (EAT) model followed by rat corneal micro-pocket and chicken chorioallantoic membrane (CAM) assays. Apoptosis induction was assessed by morphological and cell cycle analysis. RESULTS: EA extracts of E. jambolana and M. paradisiaca exhibited the highest cytotoxicity (IC 25 and 60 µg/mL), inhibited cell proliferation (up to 81%), and tube formation (83% and 76%). In vivo treatment reduced body weight (50%); cell number (16.5- and 14.7-fold), secreted VEGF (∼90%), neoangiogenesis in rat cornea (2.5- and 1.5-fold) and CAM (3- and 1.6-fold) besides EAT cells accumulation in sub-G1 phase (20% and 18.38%), respectively. DISCUSSION AND CONCLUSION: Considering the potent anti-angiogenic and pro-apoptotic properties, lead molecules from EA extracts of E. jambolana and M. paradisiaca can be developed into anticancer drugs.
[Mh] Termos MeSH primário: Inibidores da Angiogênese/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Carcinoma de Ehrlich/prevenção & controle
Membrana Corioalantoide/irrigação sanguínea
Cucurbitaceae/química
Musa/química
Neovascularização Patológica
Neovascularização Fisiológica/efeitos dos fármacos
Extratos Vegetais/farmacologia
Syzygium/química
Fator A de Crescimento do Endotélio Vascular/farmacologia
[Mh] Termos MeSH secundário: 1-Butanol/química
Acetatos/química
Inibidores da Angiogênese/isolamento & purificação
Animais
Antineoplásicos Fitogênicos/isolamento & purificação
Apoptose/efeitos dos fármacos
Carcinoma de Ehrlich/sangue
Carcinoma de Ehrlich/patologia
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Embrião de Galinha
Neovascularização da Córnea/patologia
Neovascularização da Córnea/fisiopatologia
Neovascularização da Córnea/prevenção & controle
Relação Dose-Resposta a Droga
Feminino
Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
Seres Humanos
Células MCF-7
Camundongos
Fitoterapia
Extratos Vegetais/isolamento & purificação
Folhas de Planta
Raízes de Plantas/química
Plantas Medicinais
Ratos Wistar
Sementes/química
Fatores de Tempo
Carga Tumoral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Angiogenesis Inhibitors); 0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts); 0 (VEGFA protein, human); 0 (Vascular Endothelial Growth Factor A); 76845O8NMZ (ethyl acetate); 8PJ61P6TS3 (1-Butanol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1080/13880209.2017.1307422



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