[PMID]: | 28355175 |
[Au] Autor: | Huang C; Yu Y |
[Ad] Endereço: | Department of Dentistry, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China (mainland). |
[Ti] Título: | Synergistic Cytotoxicity of ß-Elemene and Cisplatin in Gingival Squamous Cell Carcinoma by Inhibition of STAT3 Signaling Pathway. |
[So] Source: | Med Sci Monit;23:1507-1513, 2017 Mar 29. |
[Is] ISSN: | 1643-3750 |
[Cp] País de publicação: | United States |
[La] Idioma: | eng |
[Ab] Resumo: | BACKGROUND Cisplatin remains one of the most active agents and is the mainstay of combination chemotherapy regimens against gingival squamous cell carcinoma. However, the efficacy of cisplatin is limited by its high toxicity and the development of drug resistance. ß-elemene, isolated from the Chinese herb Rhizoma zedoariahas, is highly effective against malignancies and has low toxicity, but the development of ß-elemene sensitizing chemotherapy in targeting the STAT3 signaling pathway remains unexplored in gingival squamous cell carcinoma. The present study was conducted to assess the chemosensitizing effects of b-elemene for enhancing the cytotoxicity of cisplatin in gingival squamous cell carcinoma. MATERIAL AND METHODS The gingival squamous cell carcinoma YD-38 cell line was used. MTT assay, clonogenic assay, annexin V/PI apoptosis assay, Western blot analysis, and xenograft model treatment were carried out in vitro and in vivo. RESULTS ß-elemene significantly enhanced proliferative inhibition and cisplatin induced apoptosis in gingival squamous cell carcinoma. Cisplatin combined with ß-elemene decreased the expressions of p-STAT3, p-JAK2, and Bcl-2, and increased the expressions of Bax and caspase-3 significantly compared to cisplatin only treatment, as well as in the xenograft model. CONCLUSIONS The results indicated that ß-elemene promoted the anti-proliferative and apoptotic effect of cisplatin by inhibiting STAT3 and blocking the JAK2-STAT3 signaling pathway in GSCC in vitro and in vivo. |
[Mh] Termos MeSH primário: |
Carcinoma de Células Escamosas/patologia Cisplatino/farmacologia Neoplasias Gengivais/patologia Fator de Transcrição STAT3/metabolismo Sesquiterpenos/farmacologia Transdução de Sinais/efeitos dos fármacos
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[Mh] Termos MeSH secundário: |
Animais Antineoplásicos/farmacologia Apoptose/efeitos dos fármacos Linhagem Celular Tumoral Proliferação Celular/efeitos dos fármacos Relação Dose-Resposta a Droga Sinergismo Farmacológico Camundongos Ensaios Antitumorais Modelo de Xenoenxerto
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antineoplastic Agents); 0 (STAT3 Transcription Factor); 0 (Sesquiterpenes); 0 (beta-elemene); Q20Q21Q62J (Cisplatin) |
[Em] Mês de entrada: | 1704 |
[Cu] Atualização por classe: | 170424 |
[Lr] Data última revisão:
| 170424 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170330 |
[St] Status: | MEDLINE |
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