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[PMID]:29230263
[Au] Autor:Touzani MA; Yddoussalah O
[Ad] Endereço:Université Mohammed 5, Faculté de Médecine et de Pharmacie de Rabat, Hopital Ibn Sina, Service d'Urologie B, Maroc.
[Ti] Título:[Kaposi's sarcoma of the penis in a HIV-seronegative patient].
[Ti] Título:Sarcome de kaposi penien chez un patient seronegatif..
[So] Source:Pan Afr Med J;28:61, 2017.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:fre
[Ab] Resumo:Sarcomas of the penis account for less than 5% of all tumors of the penis. They are dominated by Kaposi's sarcoma that mainly affects HIV-positive patients. However, recent studies have shown a relationship between Kaposi's sarcoma and HHV-8 infection (Human herpes virus-8), which explains why this sarcoma occurs in non-immunocompromised and HIV-seronegative patients. We here report the case of a 72-year old patient, with no previous medical history, reporting of gradual onset of tumor-like granulation tissue of 3 years duration at the level of the gland, without secondary location. Given the patient's clinical condition, epidermoid carcinoma or sarcomatoid carcinoma of the penis were suspected. Initial biopsy was negative, the second was in favor of Kaposi's sarcoma, confirmed by immunohistochemistry. The patient underwent chemotherapy.
[Mh] Termos MeSH primário: Soronegatividade para HIV
Neoplasias Penianas/diagnóstico
Sarcoma de Kaposi/diagnóstico
Neoplasias Cutâneas/diagnóstico
[Mh] Termos MeSH secundário: Idoso
Antineoplásicos/uso terapêutico
Biópsia
Seres Humanos
Imuno-Histoquímica
Masculino
Neoplasias Penianas/tratamento farmacológico
Neoplasias Penianas/patologia
Sarcoma de Kaposi/tratamento farmacológico
Sarcoma de Kaposi/patologia
Neoplasias Cutâneas/tratamento farmacológico
Neoplasias Cutâneas/patologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171222
[Lr] Data última revisão:
171222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.2017.28.61.13248


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[PMID]:29230262
[Au] Autor:Touzani MA; Yddoussalah O
[Ad] Endereço:Université Mohammed 5, Faculté de Médecine et de Pharmacie de Rabat, Hopital Ibn Sina, Service d'Urologie B, Maroc.
[Ti] Título:[Cavernous metastasis from blader tumor].
[Ti] Título:Métastase caverneuse d'une tumeur de vessie..
[So] Source:Pan Afr Med J;28:60, 2017.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:fre
[Ab] Resumo:The incidence of cavernous metastases after radical treatment for bladder tumor is exceptional and is approximately 1%. The mean time between surgery and the occurrence of metastases is 8 months. In some cases they occur after 10 years. Diagnosis is confirmed by biopsy, which shows their urothelial origin. Here we report the case of 56-year old patient, with a history of smoking, having undergone total cystoprostatectomy in January 2017, who presented 2 months later with induratio penis plastica with no other sign associated. Priapism and gangrene of the penis of infectious origin have been suspected but, given the patient's clinical condition, the more likely diagnosis was cavernous metastasis. On two occasions the patient refused biopsy: during two consultations at 15 days interval (A, B). The patient accepted to undergo biopsy during the 3 consultation (C, D). Biopsy confirmed the diagnosis of cavernous metastasis.
[Mh] Termos MeSH primário: Cistectomia/métodos
Neoplasias Penianas/diagnóstico
Prostatectomia/métodos
Neoplasias da Bexiga Urinária/patologia
[Mh] Termos MeSH secundário: Biópsia
Seres Humanos
Masculino
Meia-Idade
Induração Peniana/diagnóstico
Induração Peniana/etiologia
Neoplasias Penianas/patologia
Neoplasias Penianas/secundário
Fatores de Tempo
Neoplasias da Bexiga Urinária/cirurgia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171222
[Lr] Data última revisão:
171222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.2017.28.60.13244


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[PMID]:28982864
[Au] Autor:Damasdi M; Kovacs K; Farkas N; Jakab F; Kovacs G
[Ad] Endereço:Department of Urology, Medical School, University of Pecs, Pecs, Hungary.
[Ti] Título:Down-regulation of Toll-like Receptor TLR4 Is Associated with HPV DNA Integration in Penile Carcinoma.
[So] Source:Anticancer Res;37(10):5515-5519, 2017 10.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIM: Development of penile cancers is attributed to HPV-related carcinogenesis. Our aim was to analyze HPV positivity and TLR4, p16 and p53 expression. MATERIALS AND METHODS: HPV presence was assessed with virus-specific TaqMan PCR and HPV Genotyping Test in 31 penile cancers. Immunohistochemistry was carried out on tissue microarray. RESULTS: TLR4 expression was detected in 4 of the 16 HPV positive and 13 of the 15 HPV negative tumors. We found a significant inverse correlation between HPV positivity and TLR4 expression (p=0.0006). Ten of the 16 HPV-positive but none of the 15 HPV-negative tumors expressed p16INK4a. A significant correlation was seen between p53 expression and lack of HPV DNA (p=0.0191) as well as between TLR4 and p53 expression (p=0.0198) in penile cancers. CONCLUSION: Our findings suggest a protective role of TLR4 expression against HPV DNA integration and the viral and non-viral carcinogenesis of penile cancer.
[Mh] Termos MeSH primário: Biomarcadores Tumorais/análise
Carcinoma/química
DNA Viral/genética
Papillomaviridae/genética
Infecções por Papillomavirus/virologia
Neoplasias Penianas/química
Receptor 4 Toll-Like/análise
Integração Viral
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Carcinoma/genética
Carcinoma/patologia
Carcinoma/virologia
Transformação Celular Viral
Inibidor p16 de Quinase Dependente de Ciclina/análise
Regulação para Baixo
Regulação Neoplásica da Expressão Gênica
Testes de DNA para Papilomavírus Humano
Seres Humanos
Imuno-Histoquímica
Masculino
Meia-Idade
Infecções por Papillomavirus/complicações
Neoplasias Penianas/genética
Neoplasias Penianas/patologia
Neoplasias Penianas/virologia
Análise Serial de Tecidos
Proteína Supressora de Tumor p53/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers, Tumor); 0 (Cyclin-Dependent Kinase Inhibitor p16); 0 (DNA, Viral); 0 (P16 protein, human); 0 (TLR4 protein, human); 0 (TP53 protein, human); 0 (Toll-Like Receptor 4); 0 (Tumor Suppressor Protein p53)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE


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[PMID]:28922187
[Au] Autor:Rodriguez IM; Cuevas M; Silvero A; Cañete-Portillo S; Sanchez DF; Barreto J; Cubilla AL
[Ad] Endereço:*Pathology and Research Institute †School of Medicine, National University of Asunción, Asunción, Paraguay.
[Ti] Título:Novel Histologic Finding: Adipose Tissue Is Prevalent Within Penile Tunica Albuginea and Corpora Cavernosa: An Anatomic Study of 63 Specimens and Considerations for Cancer Invasion.
[So] Source:Am J Surg Pathol;41(11):1542-1546, 2017 Nov.
[Is] ISSN:1532-0979
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Adipose tissue, along with arteries, veins, and peripheral nerves, is a normal constituent of mesenchymal tissues encasing the corpora cavernosa at the level of the penile shaft, variously designated as penile fascia or Bucks fascia. To our knowledge, the presence of fat has not been previously reported within the corpora cavernosa. One or 2 transversal histologic sections at the level of the surgical margin at the shaft of 63 consecutive partial penectomy specimens for squamous cell carcinoma were evaluated. From outer to inner tissues, 3 anatomic levels were identified: (1) outer fascia composed of a loose fibrovascular mesenchyme containing some nerve branches. Adipose tissue was present in the majority of the cases. (2) The tunica albuginea, a thick and dense fibroelastic band of tissue separating the outer fascia from the erectile tissues of the corpora cavernosa. Adipose tissue within the albuginea was present in 21 specimens (19%). (3) Erectile tissues of corpora cavernosa. Besides the typical erectile tissues, adipose tissue was present in 33 cases (52%). The fatty tissue was focal or multifocal and scant and peripherally located at the junction of the tunica albuginea with the corpora. In some cases, it was associated with small amounts of fibrous tissue, small vessels, and nerves. We are reporting the presence of adipose tissue in the tunica albuginea and the corpora cavernosa. It is possible that adipose tissue, along with small nutritional vessels and nerves perforates from the fascia, in which fat is usually present, through the tunica albuginea to reach the corpora. In a previous examination of the local routes of cancer spread, we found this pathway to be one of the mechanisms of cancer invading the penile corpora from the penile fascia.
[Mh] Termos MeSH primário: Tecido Adiposo/patologia
Carcinoma de Células Escamosas/patologia
Tecido Elástico/patologia
Neoplasias Penianas/patologia
[Mh] Termos MeSH secundário: Tecido Adiposo/cirurgia
Biópsia
Carcinoma de Células Escamosas/cirurgia
Tecido Elástico/cirurgia
Fáscia/patologia
Seres Humanos
Masculino
Invasividade Neoplásica
Neoplasias Penianas/cirurgia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170919
[St] Status:MEDLINE
[do] DOI:10.1097/PAS.0000000000000953


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[PMID]:28885326
[Au] Autor:Baraziol R; Schiavon M; Fraccalanza E; De Giorgi G
[Ad] Endereço:aAzienda Sanitaria Universitaria Integrata di Udine, Plastic Surgery Unit bAzienda Sanitaria Universitaria Integrata di Udine, Urology Unit, Udine, Italy.
[Ti] Título:Melanoma in situ of penis: a very rare entity: A case report and review of the literature.
[So] Source:Medicine (Baltimore);96(36):e7652, 2017 Sep.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Melanoma in situ of the penis is very rare and there are no clear guidelines for its surgical treatment. PATIENT CONCERNS: The authors describe the case of a 69-year-old man who presented with an asymptomatic brown macula on his glans penis and foreskin that appeared about 8 years earlier, enlarged in the last few months. DIAGNOSES: A diagnostic biopsy showed the characteristics of a melanoma in situ. INTERVENTIONS: The authors decided to excise the lesion keeping a healthy margin of 1 cm all over around except close to the urethral meatus, where it was impossible, and where only 5 mm of free margin was excised. A full thickness mucosal graft from oral cavity was performed to repair the defect. OUTCOMES: No recurrence or metastasis occurred during 50 months after the operation. LESSONS: Considering that at the sixth clinical follow-up the patient was alive and disease free at 50 months after surgery, the chosen treatment has proved successful.
[Mh] Termos MeSH primário: Melanoma/diagnóstico
Neoplasias Penianas/diagnóstico
[Mh] Termos MeSH secundário: Idoso
Seres Humanos
Masculino
Melanoma/patologia
Melanoma/cirurgia
Neoplasias Penianas/patologia
Neoplasias Penianas/cirurgia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000007652


  6 / 4564 MEDLINE  
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[PMID]:28739762
[Au] Autor:Wang S; Du P; Tang X; An C; Zhang N; Yang Y
[Ad] Endereço:Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Urological Department, Peking University Cancer Hospital & Institute, Beijing, P.R. China.
[Ti] Título:Comparison of Efficiency of Video Endoscopy and Open Inguinal Lymph Node Dissection.
[So] Source:Anticancer Res;37(8):4623-4628, 2017 08.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:AIMS: To compare the efficiency and complications of video endoscopy, inguinal lymphadenectomy and open inguinal lymph node dissection. PATIENTS AND METHODS: Nineteen video endoscopy inguinal lymphadenectomies were performed on 16 patients from September 2014 to December 2015, and 21 open inguinal lymph node dissections were performed on 18 patients from September 2013 to September 2014. Surgical time, operative blood loss, incidence of complications, length of stay, lymph node harvest and suture removal time were compared between the two groups. RESULTS: Operative time (135.5±45.52 vs. 169.8±55.19 min), lymph node harvest (10.78±5.22 vs. 12.60±5.53), and hospital stay (10.43±2.53 vs. 12.50±4.98 days) did not statistically differ between the two groups (p>0.05). However, operative blood loss (22.50±14.24 vs. 68.44±42.19 ml), drainage tube removal time (7.23±1.79 vs. 11.44±2.69 days), incidence of complications (21.05% vs. 61.9%), and suture removal time (7.52±1.24 vs. 12.81±2.04 min) were significantly reduced in the video endoscopy surgery group compared with the open surgery group (p<0.05). CONCLUSION: Compared with open surgery, video endoscopy surgery achieved the same efficiency, and had the advantage of reduction of complications, suture removal time and in-surgery blood loss.
[Mh] Termos MeSH primário: Endoscopia/métodos
Canal Inguinal/patologia
Canal Inguinal/cirurgia
Excisão de Linfonodo
Linfonodos/patologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Terapia Combinada
Endoscopia/efeitos adversos
Seres Humanos
Tempo de Internação
Excisão de Linfonodo/efeitos adversos
Excisão de Linfonodo/métodos
Masculino
Meia-Idade
Estadiamento de Neoplasias
Duração da Cirurgia
Neoplasias Penianas/diagnóstico por imagem
Neoplasias Penianas/patologia
Neoplasias Penianas/cirurgia
Cuidados Pós-Operatórios
Complicações Pós-Operatórias
Recidiva
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE


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[PMID]:28658136
[Au] Autor:Guo LC; Li G; Wang XM; Zhang M; Huang JA; Chen YB
[Ad] Endereço:aDepartment of Pathology bDepartment of Urology cDepartment of Radiology dDepartment of Respiratory Medicine, The First Affiliated Hospital of Soochow University, Suzhou, China.
[Ti] Título:Penile metastases from primary lung cancer: Case report and literature review.
[So] Source:Medicine (Baltimore);96(26):e7307, 2017 Jun.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Metastasis to the penis from primary lung cancer is quite rare. To improve the understanding, we present a case diagnosed as penile metastasis from primary lung cancer and review the literature. METHODS: One case report and retrospectively analysis penile cancer patient secondary from primary lung cancer. RESULTS: The patient complained of perineal pain and burning on urination for about 2 months. On physical examination, painful nodular masses at the base of left side of the corpora cavernosa were found. 18F-fluorodeoxyglucose positron emission tomography/CT (PET/CT) scan showed that maximum standardized uptake value (SUVmax) in left side corpora cavernosa and right hilar increased to 12.0 and 13.5 respectively. On flexible bronchoscopy checking, stenosis of the opening of apical segmental and posterior segmental bronchi of right upper lobe was found. The lateral segmental bronchi of left lower lobe was obstructed by a neoplasm. The pathological result was primary pulmonary adenosquamous carcinoma (ASC). Two months later, total penectomy was performed. The pathological result was penile ASC derived from pulmonary. On reviewing the literature, there are 39 cases reported. The patient we present is the 40 one. The average age at diagnosis was (60.5 ±â€Š10.7) years old. The most common symptom was mass, followed by priapism, pain. The overall survival time was (4.5 ±â€Š3.9) months. CONCLUSIONS: The penis may be a site of metastasis from lung cancer, especially for old patient. Metastasis to the penis usually indicates that the primary lung cancer is at an advanced stage and the prognosis is very poor.
[Mh] Termos MeSH primário: Carcinoma Adenoescamoso/secundário
Neoplasias Pulmonares/patologia
Neoplasias Penianas/secundário
[Mh] Termos MeSH secundário: Carcinoma Adenoescamoso/diagnóstico por imagem
Carcinoma Adenoescamoso/tratamento farmacológico
Carcinoma Adenoescamoso/cirurgia
Evolução Fatal
Seres Humanos
Neoplasias Pulmonares/diagnóstico por imagem
Neoplasias Pulmonares/tratamento farmacológico
Masculino
Meia-Idade
Neoplasias Penianas/diagnóstico por imagem
Neoplasias Penianas/tratamento farmacológico
Neoplasias Penianas/cirurgia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000007307


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[PMID]:28651012
[Au] Autor:Östensson E; Silfverschiöld M; Greiff L; Asciutto C; Wennerberg J; Lydryp ML; Håkansson U; Sparén P; Borgfeldt C
[Ad] Endereço:Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden.
[Ti] Título:The economic burden of human papillomavirus-related precancers and cancers in Sweden.
[So] Source:PLoS One;12(6):e0179520, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: High-risk (HR) human papillomavirus (HPV) infection is an established cause of malignant disease. We used a societal perspective to estimate the cost of HR HPV-related cervical, vulvar, vaginal, anal, and penile precancer and cancer, and oropharyngeal cancer in Sweden in 2006, 1 year before HPV vaccination became available in the country. MATERIALS AND METHODS: This prevalence-based cost-of-illness study used diagnosis-specific data from national registries to determine the number of HR HPV-related precancers and cancers. The HR HPV-attributable fractions of these diseases were derived from a literature review and applied to the total burden to estimate HR HPV-attributable costs. Direct costs were based on health care utilization and indirect costs on loss of productivity due to morbidity (i.e., sick leave and early retirement) and premature mortality. RESULTS: The total annual cost of all HR HPV-attributable precancers and cancers was €94 million (€10.3/inhabitant). Direct costs accounted for €31.3 million (€3.4/inhabitant) of the total annual cost, and inpatient care amounted to €20.7 million of direct costs. Indirect costs made up €62.6 million (€6.9/inhabitant) of the total annual cost, and premature mortality amounted to €36 million of indirect costs. Cervical precancer and cancer was most costly (total annual cost €58.4 million). Among cancers affecting both genders, anal precancer and cancer, and oropharyngeal cancer were the most costly (€11.2 million and €11.9 million, respectively). For oropharyngeal cancer, males had the highest health care utilization and represented 71% of the total annual cost. Penile precancer and cancer was least costly (€2.6 million). CONCLUSION: The economic burden of HR HPV-related precancers and cancers is substantial. The disease-related management and treatment costs we report are relevant as a point of reference for future economic evaluations investigating the overall benefits of HPV vaccination in females and males in Sweden.
[Mh] Termos MeSH primário: Neoplasias do Ânus/economia
Efeitos Psicossociais da Doença
Neoplasias dos Genitais Femininos/economia
Infecções por Papillomavirus/economia
Vacinas contra Papillomavirus/economia
Neoplasias Penianas/economia
Displasia do Colo do Útero/economia
[Mh] Termos MeSH secundário: Adulto
Neoplasias do Ânus/virologia
Feminino
Neoplasias dos Genitais Femininos/virologia
Custos de Cuidados de Saúde
Seres Humanos
Masculino
Papillomaviridae
Infecções por Papillomavirus/complicações
Infecções por Papillomavirus/virologia
Neoplasias Penianas/virologia
Suécia
Displasia do Colo do Útero/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Papillomavirus Vaccines)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170920
[Lr] Data última revisão:
170920
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179520


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[PMID]:28486384
[Au] Autor:Fernández-Nestosa MJ; Guimerà N; Sanchez DF; Cañete-Portillo S; Velazquez EF; Jenkins D; Quint W; Cubilla AL
[Ad] Endereço:*Polytechnic School §School of Medicine, National University of Asunción †Instituto de Patología e Investigación, Asunción, Paraguay ‡DDL Diagnostic Laboratory, Rijswijk, The Netherlands ∥Miraca Life Sciences and Tufts University, Boston, MA.
[Ti] Título:Human Papillomavirus (HPV) Genotypes in Condylomas, Intraepithelial Neoplasia, and Invasive Carcinoma of the Penis Using Laser Capture Microdissection (LCM)-PCR: A Study of 191 Lesions in 43 Patients.
[So] Source:Am J Surg Pathol;41(6):820-832, 2017 Jun.
[Is] ISSN:1532-0979
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Laser capture microdissection-polymerase chain reaction (LCM-PCR) supported by p16 was used for the first time to demonstrate human papillomavirus (HPV) DNA in histologically specific penile lesions, which were as follows: squamous hyperplasia (12 lesions, 10 patients), flat lesions (12 lesions, 5 patients), condylomas (26 lesions, 7 patients), penile intraepithelial neoplasia (PeIN) (115 lesions, 43 patients), and invasive squamous cell carcinomas (26 lesions, 26 patients). HPV was detected by whole-tissue section and LCM-PCR. LCM proved to be more precise than whole-tissue section in assigning individual genotypes to specific lesions. HPV was negative or very infrequent in squamous hyperplasia, differentiated PeIN, and low-grade keratinizing variants of carcinomas. HPV was strongly associated with condylomas, warty/basaloid PeIN, adjacent flat lesions, and warty/basaloid carcinomas. A single HPV genotype was found in each lesion. Some condylomas and flat lesions, especially those with atypia, were preferentially associated with high-risk HPV. Unlike invasive carcinoma, in which few genotypes of HPV were involved, there were 18 HPV genotypes in PeIN, usually HPV 16 in basaloid PeIN but marked HPV heterogeneity in warty PeIN (11 different genotypes). Variable and multiple HPV genotypes were found in multicentric PeIN, whereas unicentric PeIN was usually related to a single genotype. There was a correspondence among HPV genotypes in invasive and associated PeIN. p16 was positive in the majority of HPV-positive lesions except condylomas containing LR-HPV. p16 was usually negative in squamous hyperplasia, differentiated PeIN, and low-grade keratinizing variants of squamous cell carcinomas. In summary, we demonstrated that LCM-PCR was a superior research technique for investigating HPV genotypes in intraepithelial lesions. A significant finding was the heterogeneity of HPV genotypes in PeIN and the differential association of HPV genotypes with subtypes of PeIN. The presence of atypia and high-risk HPV in condylomas and adjacent flat lesions suggests a precursor role, and the correspondence of HPV genotypes in invasive carcinomas and associated PeIN indicates a causal relation. Data presented support the bimodal hypothesis of penile cancer carcinogenesis in HPV-driven and non-HPV-driven carcinomas and justify the current WHO pathologic classification of PeIN in special subtypes.
[Mh] Termos MeSH primário: Carcinoma in Situ/virologia
Carcinoma de Células Escamosas/virologia
Condiloma Acuminado/virologia
Microdissecção e Captura a Laser
Papillomaviridae/genética
Infecções por Papillomavirus/diagnóstico
Neoplasias Penianas/virologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Carcinoma in Situ/patologia
Carcinoma de Células Escamosas/patologia
Condiloma Acuminado/patologia
Estudos Transversais
Genótipo
Seres Humanos
Masculino
Meia-Idade
Papillomaviridae/isolamento & purificação
Infecções por Papillomavirus/complicações
Infecções por Papillomavirus/patologia
Neoplasias Penianas/patologia
Reação em Cadeia da Polimerase
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE
[do] DOI:10.1097/PAS.0000000000000821


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[PMID]:28423512
[Au] Autor:Yang ES; Willey CD; Mehta A; Crowley MR; Crossman DK; Chen D; Anderson JC; Naik G; Della Manna DL; Cooper TS; Sonpavde G
[Ad] Endereço:Department of Radiation Oncology, University of Alabama, Birmingham (UAB), Birmingham, Alabama, USA.
[Ti] Título:Kinase analysis of penile squamous cell carcinoma on multiple platforms to identify potential therapeutic targets.
[So] Source:Oncotarget;8(13):21710-21718, 2017 Mar 28.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Penile squamous cell carcinoma (PSCC) is an orphan malignancy with poorly understood biology and suboptimal systemic therapy. Given that kinases may be drivers and readily actionable, we performed comprehensive multiplatform analysis of kinases in PSCC tumor and normal tissue. Fresh frozen tumors were collected from 11 patients with PSCC. After macrodissection to demarcate tumor from normal tissue, the samples underwent multiplatform analysis of kinases. Next Generation Sequencing (NGS) of 517 kinase genes was performed using Agilent Kinome capture and run on the Illumina MiSeq at PE150bp. The NanoString nCounter® platform analyzed the expression of 519 kinase genes. Kinase activity of tissue lysates was measured using PamStation®12 high-content phospho-peptide substrate microarray system. Network mapping was done with GeneGo MetaCore™ and upstream kinase prediction was performed with BioNavigator and the Kinexus database. Ingenuity pathway analysis was performed to integrate elevated kinase activity and gene over-expression with coexisting missense mutations at DNA level. Top pathways upregulated in both the kinase activity and gene expression platforms were PTEN, STAT3, GNRH, IL-8 and B cell receptor signaling. Potentially relevant missense mutations were seen in 176 kinase genes, with the top altered pathways overlapping with gene overexpression being GNRH, NF-kB and STAT3 signaling. ERBB2, ERBB3 and SYK were altered on NGS and also exhibited elevated kinase activity. To summarize, multiplatform comprehensive analysis of kinases discovered potential drivers of PSCC and actionable therapeutic targets. Translational studies are necessary to validate the functional relevance of our data to make advances in this rare malignancy.
[Mh] Termos MeSH primário: Carcinoma de Células Escamosas/enzimologia
Neoplasias Penianas/enzimologia
Proteínas Quinases/análise
[Mh] Termos MeSH secundário: Idoso
Carcinoma de Células Escamosas/patologia
Biologia Computacional
Análise Mutacional de DNA
Perfilação da Expressão Gênica
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Masculino
Meia-Idade
Mutação de Sentido Incorreto
Neoplasias Penianas/patologia
Proteínas Quinases/genética
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.7.- (Protein Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170421
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.15558



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