[PMID]: | 29458667 |
[Au] Autor: | Atrisco-Morales J; Martínez-Santos VI; Román-Román A; Alarcón-Millán J; De Sampedro-Reyes J; Cruz-Del Carmen I; Martínez-Carrillo DN; Fernández-Tilapa G |
[Ad] Endereço: | 1​Laboratorio de Investigación Clínica, Facultad de Ciencias Químico-Biológicas, Universidad Autónoma de Guerrero, Av. Lázaro Cárdenas s/n C.U. Sur. Chilpancingo, Guerrero, C.P. 39090, Mexico. |
[Ti] Título: | vacA s1m1 genotype and cagA EPIYA-ABC pattern are predominant among Helicobacter pylori strains isolated from Mexican patients with chronic gastritis. |
[So] Source: | J Med Microbiol;67(3):314-324, 2018 Mar. |
[Is] ISSN: | 1473-5644 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | PURPOSE: Virulent genotypes of Helicobacter pylori vacA s1m1/cagA /babA2 have been associated with severe gastric diseases. VacA, CagA and BabA are polymorphic proteins, and their association with the disease is allele-dependent. The aims of this work were: (i) to determine the prevalence of H. pylori by type of chronic gastritis; (ii) to describe the frequency of cagA, babA2 and vacA genotypes in strains from patients with different types of chronic gastritis; (iii) to characterize the variable region of cagA alleles. METHODOLOGY: A total of 164 patients with chronic gastritis were studied. Altogether, 50 H. pylori strains were isolated, and the status of cagA, babA2 and vacA genotypes was examined by PCR. cagA EPIYA segment identification was performed using PCR and sequencing of cagA fragments of six randomly selected strains.Results/Key findings. The overall prevalence of H. pylori was 30.5 %. Eighty percent of the isolated strains were vacA s1m1, and the cagA and babA2 genes were detected in 74 and 32 % of the strains, respectively. The most frequent genotypes were vacA s1m1/cagA /babA2 and vacA s1m1/cagA /babA2 , with 40 % (20/50) and 28 % (14/50), respectively. In cagA , the most frequent EPIYA motif was -ABC (78.4 %), and EPIYA-ABCC and -ABCCC motifs were found in 10.8 % of the strains. A modified EPIYT-B motif was found in 66.6 % of the sequenced strains. CONCLUSION: H. pylori strains carrying vacA s1m1, cagA and babA2 genotypes were the most prevalent in patients with chronic gastritis from the south of Mexico. In the cagA strains, the EPIYA-ABC motif was the most common. |
[Mh] Termos MeSH primário: |
Antígenos de Bactérias/genética Proteínas de Bactérias/genética Gastrite/microbiologia Infecções por Helicobacter/microbiologia Helicobacter pylori/genética
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[Mh] Termos MeSH secundário: |
Adulto Idoso Alelos Doença Crônica/epidemiologia Feminino Gastrite/epidemiologia Gastroscopia Genótipo Helicobacter pylori/isolamento & purificação Seres Humanos Masculino México/epidemiologia Meia-Idade Reação em Cadeia da Polimerase Estômago/microbiologia Estômago/patologia
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Antigens, Bacterial); 0 (Bacterial Proteins); 0 (VacA protein, Helicobacter pylori); 0 (cagA protein, Helicobacter pylori) |
[Em] Mês de entrada: | 1803 |
[Cu] Atualização por classe: | 180308 |
[Lr] Data última revisão:
| 180308 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 180221 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1099/jmm.0.000660 |
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