Base de dados : MEDLINE
Pesquisa : C07.320.830.700 [Categoria DeCS]
Referências encontradas : 2127 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 213 ir para página                         

  1 / 2127 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Aguiar, Maria Cássia Ferreira
Texto completo SciELO Brasil
[PMID]:29267664
[Au] Autor:Naufel AO; Aguiar MCF; Madeira FM; Abreu LG
[Ad] Endereço:Universidade Federal de Minas Gerais - UFMG, School of Dentistry, Department of Oral Surgery and Pathology, Belo Horizonte, MG, Brazil.
[Ti] Título:Treg and Th17 cells in inflammatory periapical disease: a systematic review.
[So] Source:Braz Oral Res;31:e103, 2017 Dec 18.
[Is] ISSN:1807-3107
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The process involved in periapical lesions, which occur as an outcome of pulpal necrosis, is regulated by the immune system including regulatory T cells (Treg) and T helper 17 cell (Th17) responses. The objective of this study was to conduct a frequency systematic review to determine the presence of Treg/Th17 responses and the influence of these cells in the progression of chronic inflammatory periapical lesions in humans. A systematic computerized search was carried out in Pubmed, Medline, Web of Science and Scopus electronic databases from their date of inception through the first week of May 2017. In addition, the reference lists of the included articles and the grey literature were hand-searched. Articles that evaluated the presence and influence of Treg/Th17 in the progression of human periapical lesions were included. Study selection and the quality assessment of the included articles (using the Newcastle-Ottawa scale) were carried out by two authors. Fifty-seven titles/abstracts were screened and eight studies met the eligibility criteria and were included in this systematic review. The included studies showed large variation in the type of periapical lesion assessed, mean age, age range, type of experiment and findings regarding the participation of Th17 and Treg in the status of inflammatory periapical lesions. The studies showed the involvement of Treg in the modulation of the inflammatory response in radicular cysts and periapical granulomas. This systematic review highlights the relationship between Treg and Th17 acting in a subtle balance inhibiting or promoting the progression of human periapical lesions.
[Mh] Termos MeSH primário: Periodontite Periapical/patologia
Linfócitos T Reguladores/patologia
Células Th17/patologia
[Mh] Termos MeSH secundário: Doença Crônica
Citocinas/análise
Progressão da Doença
Fatores de Transcrição Forkhead/análise
Seres Humanos
Periodontite Periapical/imunologia
Viés de Publicação
Linfócitos T Reguladores/imunologia
Células Th17/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cytokines); 0 (FOXP3 protein, human); 0 (Forkhead Transcription Factors)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:171222
[St] Status:MEDLINE


  2 / 2127 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Silva, Léa Assed Bezerra da
Consolaro, Alberto
Texto completo SciELO Brasil
[PMID]:29211282
[Au] Autor:Pucinelli CM; Silva LABD; Cohenca N; Romualdo PC; Silva RABD; Consolaro A; Queiroz AM; Nelson P
[Ad] Endereço:Universidade de São Paulo, Faculdade de Odontologia de Ribeirão Preto, Departamento de Clínica Infantil, Ribeirão Preto, SP, Brasil.
[Ti] Título:Apical Negative Pressure irrigation presents tissue compatibility in immature teeth.
[So] Source:J Appl Oral Sci;25(6):612-619, 2017 Nov-Dec.
[Is] ISSN:1678-7765
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:AIM: To compare the apical negative pressure irrigation (ANP) with conventional irrigation in the teeth of immature dogs with apical periodontitis. METHODS: Fifty-two immature pre-molar root canals were randomly assigned into 4 groups: ANP (n=15); conventional irrigation (n=17); healthy teeth (control) (n = 10); and teeth with untreated apical periodontitis (control) (n=10). After induction of apical periodontitis, teeth were instrumented using EndoVac® (apical negative pressure irrigation) or conventional irrigation. The animals were euthanized after 90 days. The sections were stained by HE and analyzed under conventional and fluorescence microscopy. TRAP histoenzymology was also performed. Statistical analyses were performed with the significance level set at 5%. RESULTS: There was difference in the histopathological parameters between ANP and conventional groups (p<0.05). The ANP group showed a predominance of low magnitude inflammatory infiltrate, a smaller periodontal ligament, and lower mineralized tissue resorption. There were no differences in the periapical lesion extensions between the ANP and conventional groups (p>0.05). However, a lower number of osteoclasts was observed in the ANP group (p<0.05). CONCLUSION: The EndoVac® irrigation system presented better biological results and more advanced repair process in immature teeth with apical periodontitis than the conventional irrigation system, confirming the hypothesis.
[Mh] Termos MeSH primário: Periodontite Periapical/tratamento farmacológico
Irrigantes do Canal Radicular/farmacologia
Irrigação Terapêutica/métodos
[Mh] Termos MeSH secundário: Animais
Cães
Microscopia de Fluorescência
Periodontite Periapical/patologia
Distribuição Aleatória
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Root Canal Irrigants)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180227
[Lr] Data última revisão:
180227
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:171207
[St] Status:MEDLINE


  3 / 2127 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29293651
[Au] Autor:Rôças IN; Siqueira JF
[Ad] Endereço:Department of Endodontics and Molecular Microbiology Laboratory, Estácio de Sá University, Rio de Janeiro, RJ, Brazil.
[Ti] Título:Frequency and levels of candidate endodontic pathogens in acute apical abscesses as compared to asymptomatic apical periodontitis.
[So] Source:PLoS One;13(1):e0190469, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Acute apical abscess is caused by bacteria that leave the infected dental root canal to invade the periodontal tissues. Most species occurring in abscesses are also found in asymptomatic infections; therefore, the possibility exists that not only the presence of certain species but also their specific counts influence the appearance of symptoms. This molecular study compared the frequency and levels of several candidate endodontic pathogens in teeth with acute apical abscesses and asymptomatic apical periodontitis. METHODS: Samples were taken from the root canals of teeth with asymptomatic apical periodontitis (n = 73) and by aspiration of purulent exudate from acute abscesses (n = 55). DNA was extracted from samples and bacterial identifications were performed by a closed-ended semi-quantitative reverse-capture checkerboard approach targeting 40 bacterial species/phylotypes. RESULTS: Bacterial DNA was detected in all cases. In abscesses, the most prevalent taxa were Fusobacterium nucleatum (60%), Porphyromonas endodontalis (53%), Parvimonas micra (51%), and Streptococcus species (45%). The most frequently detected taxa in asymptomatic teeth were P. endodontalis (63%), Dialister invisus (58%), Olsenella uli (56%), and F. nucleatum (51%). None of the targeted taxa were significantly associated with abscesses when only mere presence was evaluated (p>0.05). However, semi-quantitative data demonstrated that P. endodontalis, Prevotella baroniae, Treponema denticola and Streptococcus species were significantly more frequent at levels >105 in abscesses than in asymptomatic cases (p<0.05). CONCLUSION: None of the target species/phylotypes were associated with abscesses in terms of frequency. However, some taxa were significantly found in higher levels in abscesses. Presence of a potentially virulent pathogen in high counts may increase the collective pathogenicity of the bacterial community and give rise to symptoms.
[Mh] Termos MeSH primário: Abscesso/microbiologia
Bactérias/isolamento & purificação
Periodontite Periapical/microbiologia
Ápice Dentário/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Bactérias/classificação
Feminino
Seres Humanos
Masculino
Meia-Idade
Adulto Jovem
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180103
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190469


  4 / 2127 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29235383
[Au] Autor:Beach DA
[Ti] Título:Advances in Biomechanical Preparation.
[So] Source:Dent Today;36(3):86, 88-9, 2017 Mar.
[Is] ISSN:8750-2186
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Periodontite Periapical/cirurgia
Pulpite/cirurgia
Preparo de Canal Radicular/métodos
[Mh] Termos MeSH secundário: Adulto
Fenômenos Biomecânicos
Instrumentos Odontológicos
Feminino
Seres Humanos
Masculino
Meia-Idade
Radiografia Dentária
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:171214
[St] Status:MEDLINE


  5 / 2127 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:29185310
[Au] Autor:McCormack B
[Ti] Título:CBCT Imaging's Insight Into Endodontic Retreatment Success.
[So] Source:Dent Today;35(9):98-100, 2016 Sep.
[Is] ISSN:8750-2186
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Tomografia Computadorizada de Feixe Cônico
Abscesso Periapical/diagnóstico por imagem
Abscesso Periapical/cirurgia
Periodontite Periapical/diagnóstico por imagem
Periodontite Periapical/cirurgia
[Mh] Termos MeSH secundário: Adulto
Seres Humanos
Masculino
Meia-Idade
Complicações Pós-Operatórias/diagnóstico por imagem
Complicações Pós-Operatórias/cirurgia
Retratamento
Tratamento do Canal Radicular/efeitos adversos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171221
[Lr] Data última revisão:
171221
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE


  6 / 2127 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28953630
[Au] Autor:Zhang W; Wang J; Yu X; Wang W
[Ad] Endereço:aDepartment of Periodontology and Oral Mucosal Diseases bDepartment of Restorative Dentistry and Endodontics cThe Affiliated Hospital of Qingdao University Medical College, Qingdao, China.
[Ti] Título:Orofacial granulomatosis: A case report of three cases may be caused by apical periodontitis.
[So] Source:Medicine (Baltimore);96(39):e8102, 2017 Sep.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Orofacial granulomatosis (OFG) is a rare disease characterized by noncaseating granulomatous inflammation. The most common clinical presentation is persistent swelling of the soft tissues in the oral and maxillofacial regions. The precise cause of OFG is unknown. Corticosteroids are the first-line and best treatment, but there is lack of uniform treatment prescription and standard. It is important to identify the pathogen in order to improve treatment specificity. PATIENT CONCERNS: Three patients presented with recurring lip swelling and cobblestone formation on buccal mucosa, complained of toothache or dental caries for many years. They had very similar and characteristic clinical signs, especially the corresponding location with infected teeth, which suffered from apical periodontitis. DIAGNOSES: The three patients were all diagnosed with typical clinical signs and non-caseating epithelioid cell granulomas histologically. INTERVENTIONS: The teeth with apical periodontitis were extracted or treated and corticosteroids were prescribed locally or/and systematically. OUTCOMES: A complete resolution of lip swelling and cobblestone formation were shown after treatment. LESSONS: This is the first report to highlight that apical periodontitis may intrigue the pathogenesis of OFG, which suggested that dental infection may be the direct and initial etiology of OFG. Removal of infected teeth should be performed as soon as possible in order to reduce the dosage of corticosteroids and occcurence rate of OFG.
[Mh] Termos MeSH primário: Granulomatose Orofacial/etiologia
Periodontite Periapical/complicações
[Mh] Termos MeSH secundário: Adulto
Feminino
Glucocorticoides/uso terapêutico
Granulomatose Orofacial/terapia
Seres Humanos
Masculino
Metilprednisolona/uso terapêutico
Meia-Idade
Periodontite Periapical/terapia
Extração Dentária
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glucocorticoids); X4W7ZR7023 (Methylprednisolone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170928
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008102


  7 / 2127 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28617403
[Au] Autor:Konoplya AI; Goldobin DD; Loktionov AL
[Ad] Endereço:Kursk State Medical University, Kursk, Russia.
[Ti] Título:[Local immune and oxidative status in exacerbated chronic apical periodontitis].
[Ti] Título:Mestnyi immunnyi i oksidantnyi status pri khronicheskom periodontite v stadii obostreniia..
[So] Source:Stomatologiia (Mosk);96(3):26-29, 2017.
[Is] ISSN:0039-1735
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:The aim of the study was to define local immune and oxidative changes in patients with exacerbated chronic apical periodontitis. These changes were assessed in saliva of 67 patients with the mean age of 31±2.5 before and after treatment. The study revealed disturbances in cytokines and complement system balance and activation of lipids peroxidation. Combination of Gepon or Vobenzim with Essentiale forte H and Kaskatol proved to be the most effective for correction of this imbalance.
[Mh] Termos MeSH primário: Periodontite Crônica/imunologia
Periodontite Crônica/metabolismo
Estresse Oxidativo
Periodontite Periapical/imunologia
Periodontite Periapical/metabolismo
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/uso terapêutico
Adulto
Antioxidantes/uso terapêutico
Periodontite Crônica/tratamento farmacológico
Proteínas do Sistema Complemento/imunologia
Citocinas/imunologia
Progressão da Doença
Combinação de Medicamentos
Quimioterapia Combinada
Feminino
Seres Humanos
Hidrolases/uso terapêutico
Peroxidação de Lipídeos
Masculino
Oligopeptídeos/uso terapêutico
Periodontite Periapical/tratamento farmacológico
Fosfatidilcolinas/uso terapêutico
Rutina/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antioxidants); 0 (Cytokines); 0 (Drug Combinations); 0 (Oligopeptides); 0 (Phosphatidylcholines); 0 (Thr-Glu-Lys-Lys-Arg-Arg-Glu-Thr-Val-Glu-Arg-Glu-Lys-Glu); 11137-48-7 (essential 303 forte); 5G06TVY3R7 (Rutin); 72506-65-1 (Wobenzym); 9007-36-7 (Complement System Proteins); EC 3.- (Hydrolases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170810
[Lr] Data última revisão:
170810
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.17116/stomat201796326-29


  8 / 2127 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28415948
[Au] Autor:Alagl A; Bedi S; Hassan K; AlHumaid J
[Ad] Endereço:1 Division of Periodontics, Department of Preventive Dental Sciences, College of Dentistry, University of Dammam, Dammam, Saudi Arabia.
[Ti] Título:Use of platelet-rich plasma for regeneration in non-vital immature permanent teeth: Clinical and cone-beam computed tomography evaluation.
[So] Source:J Int Med Res;45(2):583-593, 2017 Apr.
[Is] ISSN:1473-2300
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Objective This study was performed to assess the clinical and radiological outcomes of a revascularization procedure in immature teeth with apical periodontitis using platelet-rich plasma (PRP). The PRP protocol and conventional revascularization protocol, which used a blood clot as the scaffold, were compared. Methods Thirty non-vital immature permanent teeth were randomly categorized into two groups. After disinfecting the root canal space with triple antibiotic paste (1:1:1 ciprofloxacin, metronidazole, and cefaclor), a tissue scaffold was created using either PRP or a blood clot (control) and covered with white mineral trioxide aggregate. All cases were followed up clinically and radiographically for 12 months. Differences in bone density, root length, and lesion size were calculated using preoperative and postoperative computed tomography images. The means of the differences in individual parameters in the blood clot and PRP groups were compared using the Mann-Whitney U test. Results After 5 months, sensitivity tests (cold and electric pulp tests) elicited a delayed positive response in 23 sites. At 12 months, cone-beam computed tomography revealed resolution or a decrease in lesion size and an increase in bone density in all 30 (100%) teeth. Additionally, continued root development was observed in 22 (73%) teeth and early root growth was observed in the test group (mineral trioxide aggregate with PRP). Conclusions The results of this study suggest that PRP can serve as a successful scaffold for regenerative endodontic treatment. With the exception of a significant increase in root length, the results of treatment with PRP were not significantly different from those of the conventional protocol using a blood clot as the scaffold.
[Mh] Termos MeSH primário: Neovascularização Fisiológica
Periodontite Periapical/cirurgia
Plasma Rico em Plaquetas/química
Regeneração/fisiologia
Trombose/metabolismo
Raiz Dentária/cirurgia
[Mh] Termos MeSH secundário: Compostos de Alumínio/uso terapêutico
Antibacterianos/farmacologia
Densidade Óssea/efeitos dos fármacos
Compostos de Cálcio/uso terapêutico
Cefaclor/farmacologia
Criança
Ciprofloxacino/farmacologia
Tomografia Computadorizada de Feixe Cônico
Cimentos Dentários/uso terapêutico
Combinação de Medicamentos
Feminino
Seguimentos
Seres Humanos
Masculino
Metronidazol/farmacologia
Óxidos/uso terapêutico
Periodontite Periapical/diagnóstico por imagem
Periodontite Periapical/patologia
Silicatos/uso terapêutico
Tecidos Suporte
Raiz Dentária/irrigação sanguínea
Raiz Dentária/diagnóstico por imagem
Raiz Dentária/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aluminum Compounds); 0 (Anti-Bacterial Agents); 0 (Calcium Compounds); 0 (Dental Cements); 0 (Drug Combinations); 0 (Oxides); 0 (Silicates); 0 (mineral trioxide aggregate); 140QMO216E (Metronidazole); 5E8K9I0O4U (Ciprofloxacin); 69K7K19H4L (Cefaclor)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1177/0300060517692935


  9 / 2127 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28397949
[Au] Autor:Al-Nazhan SA; Alsaeed SA; Al-Attas HA; Dohaithem AJ; Al-Serhan MS; Al-Maflehi NS
[Ad] Endereço:Department of Restorative Dental Science, Division of Endodontics, College of Dentistry, King Saud University, Riyadh, Kingdom of Saudi Arabia. E-mail. snazhan@ksu.edu.sa.
[Ti] Título:Prevalence of apical periodontitis and quality of root canal treatment in an adult Saudi population.
[So] Source:Saudi Med J;38(4):413-421, 2017 Apr.
[Is] ISSN:0379-5284
[Cp] País de publicação:Saudi Arabia
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To determine the health status of periradicular tissue and the quality of root canal fillings in an adult Saudi population attending dental clinics for the first time. Methods: This cross sectional study was conducted  in the dental clinics at King Saud University, Riyadh and other dental centers (Jeddah, Najran, and Albaha City), Kingdom of Saudi Arabia between year 2010   and 2012. Good-quality panoramic radiographs of 926 Saudi subjects (540 males and 386 females)were analyzed based on the gender, age, health status, smoking habits, periapical status of the endodontically treated teeth, technical quality of the root canal fillings, and the presence or absence of coronal restoration. Data were calibrated and statistical analyses were performed using the Statistical Package for the Social Sciences (SPSS Inc., Chicago, IL, USA) version 17.  Results: Of the 25,028 teeth examined, 1,556 teeth (6.2%) had apical periodontitis (AP). Male subjects aged over 55 years and females between 36 and 45 years had higher AP. A total of 36 diabetic and 87 smokers subjects had AP. The AP was more common in male diabetics than female (p=0.383), and in female smokers more than male (p=0.44). Only 42.2% of male and 57.7% of female teeth had adequate root canal treatment.  Conclusions: Apical periodontitis was significantly related to diabetes, smoking, and inadequate endodontic treatment.
[Mh] Termos MeSH primário: Restauração Dentária Permanente/normas
Periodontite Periapical/epidemiologia
Tratamento do Canal Radicular/normas
[Mh] Termos MeSH secundário: Adolescente
Adulto
Fatores Etários
Estudos Transversais
Diabetes Mellitus/epidemiologia
Feminino
Seres Humanos
Masculino
Meia-Idade
Prevalência
Qualidade da Assistência à Saúde
Radiografia Panorâmica
Fatores de Risco
Arábia Saudita/epidemiologia
Fatores Sexuais
Fumar/epidemiologia
Dente não Vital/epidemiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170412
[St] Status:MEDLINE
[do] DOI:10.15537/smj.2017.4.16409


  10 / 2127 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28365680
[Au] Autor:Wang F; Jiang Y; Huang X; Liu Q; Zhang Y; Luo W; Zhang F; Zhou P; Lin J; Zhang H
[Ad] Endereço:Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, the Affiliated Hospital of Stomatology of Chongqing Medical University, Chongqing, China.
[Ti] Título:Pro-Inflammatory Cytokine TNF-α Attenuates BMP9-Induced Osteo/ Odontoblastic Differentiation of the Stem Cells of Dental Apical Papilla (SCAPs).
[So] Source:Cell Physiol Biochem;41(5):1725-1735, 2017.
[Is] ISSN:1421-9778
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIMS: Periapical periodontitis is a common oral disease caused by bacterial invasion of the tooth pulp, which usually leads to local release of pro-inflammatory cytokines and osteolytic lesion. This study is intended to examine the effect of TNF-α on BMP9-induced osteogenic differentiation of the stem cells of dental apical papilla (SCAPs). METHODS: Rat model of periapical periodontitis was established. TNF-α expression was assessed. Osteogenic markers and ectopic bone formation in iSCAPs were analyzed upon BMP9 and TNF-α treatment. RESULTS: Periapical periodontitis was successfully established in rat immature permanent teeth with periapical lesions, in which TNF-α was shown to release during the inflammatory phase. BMP9-induced alkaline phosphatase activity, the expression of osteocalcin and osteopontin, and matrix mineralization in iSCAPs were inhibited by TNF-α in a dose-dependent fashion, although increased AdBMP9 partially overcame TNF-α inhibition. Furthermore, high concentration of TNF-α effectively inhibited BMP9-induced ectopic bone formation in vivo. CONCLUSION: TNF-α plays an important role in periapical bone defect during the inflammatory phase and inhibits BMP9-induced osteoblastic differentiation of iSCAPs, which can be partially reversed by high levels of BMP9. Therefore, BMP9 may be further explored as a potent osteogenic factor to improve osteo/odontogenic differentiation in tooth regeneration in chronic inflammation conditions.
[Mh] Termos MeSH primário: Diferenciação Celular
Fator 2 de Diferenciação de Crescimento/metabolismo
Odontoblastos/metabolismo
Periodontite Periapical/metabolismo
Células-Tronco/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Mh] Termos MeSH secundário: Fosfatase Alcalina/biossíntese
Animais
Indução Enzimática
Masculino
Odontoblastos/patologia
Periodontite Periapical/patologia
Ratos
Ratos Sprague-Dawley
Células-Tronco/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Growth Differentiation Factor 2); 0 (Tumor Necrosis Factor-alpha); EC 3.1.3.1 (Alkaline Phosphatase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170403
[St] Status:MEDLINE
[do] DOI:10.1159/000471865



página 1 de 213 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde