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[PMID]:29390529
[Au] Autor:Wang Y; Li W; Shi L; Zhang F; Zheng S
[Ad] Endereço:Department of Periodontology.
[Ti] Título:Comparison of clinical parameters, microbiological effects and calprotectin counts in gingival crevicular fluid between Er: YAG laser and conventional periodontal therapies: A split-mouth, single-blinded, randomized controlled trial.
[So] Source:Medicine (Baltimore);96(51):e9367, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The erbium-doped yttrium, aluminum, and garnet (Er:YAG) laser is thought to be the most promising laser for periodontal treatment; however, its application is still under consideration. The aim of this study was to compare Er:YAG laser monotherapy with conventional scaling and root planing (SRP) for chronic periodontitis using clinical parameters, the detection rate of periodontal pathogens, and the calprotectin level in gingival crevicular fluid. METHODS: Twenty-seven participants with moderate-to-advanced chronic periodontitis were included. In a split-mouth design, the 2 half-mouths of each participant were randomly assigned to Er:YAG laser or SRP (combination of ultrasonic and manual instruments) treatment. Clinical parameters were recorded at baseline, 6 weeks, and 3 and 6 months after treatment. At the same time points, gingival crevicular fluid was collected to analyze the detection rate of 6 periodontal pathogens by polymerase chain reaction and the levels of calprotectin by enzyme-linked immunosorbent assay. RESULTS: Both treatment groups showed significant reductions in probing depth (PD), bleeding index (BI), and clinical attachment level (CAL) from baseline to 6 months. For sites with 4 mm ≤ PD ≤ 6 mm at baseline, SRP resulted in a greater reduction in PD and CAL than Er:YAG laser treatment, and the difference remained at 6 months post-treatment (P = .01 and P < .01, respectively). For sites with PD ≥7 mm at baseline, the clinical parameters showed similar results between the 2 groups. SRP resulted in a lower detection rate of Porphyromonas gingivalis at 6 months post-treatment. The levels of calprotectin were significantly decreased from baseline to 6 months in both groups, without a significant difference between the groups. CONCLUSION: For mild pockets, conventional SRP may still be the preferred choice. For deep pockets, Er:YAG laser treatment could be an effective alternative. Studies are needed to explore more advanced instruments and new application methods for the Er:YAG laser for periodontal treatment in deep pockets.
[Mh] Termos MeSH primário: Periodontite Crônica/terapia
Líquido do Sulco Gengival/microbiologia
Lasers de Estado Sólido/uso terapêutico
Complexo Antígeno L1 Leucocitário/metabolismo
Bolsa Periodontal/radioterapia
Aplainamento Radicular/métodos
[Mh] Termos MeSH secundário: Adulto
Periodontite Crônica/diagnóstico
Terapia Combinada
Ensaio de Imunoadsorção Enzimática
Feminino
Seguimentos
Seres Humanos
Masculino
Meia-Idade
Índice Periodontal
Medição de Risco
Método Simples-Cego
Fatores de Tempo
Resultado do Tratamento
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
0 (Leukocyte L1 Antigen Complex)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009367


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[PMID]:28800622
[Au] Autor:Belstrøm D; Sembler-Møller ML; Grande MA; Kirkby N; Cotton SL; Paster BJ; Holmstrup P
[Ad] Endereço:Section for Periodontology, Microbiology, and Community Dentistry, Department of Odontology, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark.
[Ti] Título:Microbial profile comparisons of saliva, pooled and site-specific subgingival samples in periodontitis patients.
[So] Source:PLoS One;12(8):e0182992, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The purpose of this study was to compare microbial profiles of saliva, pooled and site-specific subgingival samples in patients with periodontitis. We tested the hypotheses that saliva can be an alternative to pooled subgingival samples, when screening for presence of periopathogens. DESIGN: Site specific subgingival plaque samples (n = 54), pooled subgingival plaque samples (n = 18) and stimulated saliva samples (n = 18) were collected from 18 patients with generalized chronic periodontitis. Subgingival and salivary microbiotas were characterized by means of HOMINGS (Human Oral Microbe Identification using Next Generation Sequencing) and microbial community profiles were compared using Spearman rank correlation coefficient. RESULTS: Pronounced intraindividual differences were recorded in site-specific microbial profiles, and site-specific information was in general not reflected by pooled subgingival samples. Presence of Porphyromonas gingivalis, Treponema denticola, Prevotella intermedia, Filifactor alocis, Tannerella forsythia and Parvimona micra in site-specific subgingival samples were detected in saliva with an AUC of 0.79 (sensitivity: 0.61, specificity: 0.94), compared to an AUC of 0.76 (sensitivity: 0.56, specificity: 0.94) in pooled subgingival samples. CONCLUSIONS: Site-specific presence of periodontal pathogens was detected with comparable accuracy in stimulated saliva samples and pooled subgingival plaque samples. Consequently, saliva may be a reasonable surrogate for pooled subgingival samples when screening for presence of periopathogens. Future large-scale studies are needed to confirm findings from this study.
[Mh] Termos MeSH primário: DNA Bacteriano/genética
Filogenia
Porphyromonas gingivalis/genética
Prevotella intermedia/genética
Tannerella forsythia/genética
Treponema denticola/genética
[Mh] Termos MeSH secundário: Adulto
Idoso
Periodontite Crônica/diagnóstico
Periodontite Crônica/microbiologia
Placa Dentária/diagnóstico
Placa Dentária/microbiologia
Feminino
Sequenciamento de Nucleotídeos em Larga Escala
Seres Humanos
Masculino
Meia-Idade
Bolsa Periodontal/diagnóstico
Bolsa Periodontal/microbiologia
Porphyromonas gingivalis/classificação
Porphyromonas gingivalis/isolamento & purificação
Prevotella intermedia/classificação
Prevotella intermedia/isolamento & purificação
Saliva/química
Saliva/microbiologia
Tannerella forsythia/classificação
Tannerella forsythia/isolamento & purificação
Treponema denticola/classificação
Treponema denticola/isolamento & purificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182992


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[PMID]:28678946
[Au] Autor:Gomes CC; Guimarães LS; Pinto LCC; Camargo GADCG; Valente MIB; Sarquis MIM
[Ad] Endereço:Universidade Federal Fluminense, Faculdade de Odontologia, Departamento de Formação Específica, Nova Friburgo, RJ, Brasil.
[Ti] Título:Investigations of the prevalence and virulence of Candida albicans in periodontal and endodontic lesions in diabetic and normoglycemic patients.
[So] Source:J Appl Oral Sci;25(3):274-281, 2017 May-Jun.
[Is] ISSN:1678-7765
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Objective: The aim of this study was to investigate the prevalence of isolated Candida albicans from periodontal endodontic lesions in diabetic and normoglycemic patients, and the fungi's virulence in different atmospheric conditions. Material and Methods: A case-control study was conducted on 15 patients with type 2 diabetes mellitus (G1) and 15 non-diabetics (G2) with periodontal endodontic lesions. Samples of root canals and periodontal pockets were plated on CHROMagar for later identification by polymerase chain reaction (PCR) and virulence test. Results: C. albicans was identified in 79.2% and 20.8% of the 60 samples collected from diabetic and normoglycemic patients, respectively. Of the 30 samples collected from periodontal pockets, 13 showed a positive culture for C. albicans, with 77% belonging to G1 and 23% to G2. Of the 11 positive samples from root canals, 82% were from G1 and 18% from G2. Production of proteinase presented a precipitation zone Pz<0.63 of 100% in G1 and 72% in G2, in redox and negative (Pz=1), under anaerobic conditions in both groups. Hydrophobicity of the strains from G1 indicated 16.4% with low, 19.3% with moderate, and 64.3% with high hydrophobicity in redox. In G2, 42.2% had low, 39.8% had moderate, 18% had high hydrophobicity in redox. In anaerobic conditions, G1 showed 15.2% with low, 12.8% with moderate, and 72% with high hydrophobicity; in G2, 33.6% had low, 28.8% had moderate, and 37.6% had high hydrophobicity. There was statistical difference in the number of positive cultures between G1 and G2 (p<0.05) with predominance in G1. There was statistical difference for all virulence factors, except hemolysis (p=0.001). Conclusions: Candida albicans was isolated more frequently and had higher virulence in diabetic patients.
[Mh] Termos MeSH primário: Candida albicans/isolamento & purificação
Candida albicans/patogenicidade
Doenças da Polpa Dentária/microbiologia
Diabetes Mellitus Tipo 2/microbiologia
Doenças Periodontais/microbiologia
[Mh] Termos MeSH secundário: Adulto
Idoso
Estudos de Casos e Controles
DNA Fúngico
Cavidade Pulpar/microbiologia
Doenças da Polpa Dentária/diagnóstico por imagem
Doenças da Polpa Dentária/fisiopatologia
Diabetes Mellitus Tipo 2/fisiopatologia
Eletroforese
Feminino
Seres Humanos
Interações Hidrofóbicas e Hidrofílicas
Masculino
Meia-Idade
Oxirredução
Peptídeo Hidrolases/análise
Doenças Periodontais/diagnóstico por imagem
Doenças Periodontais/fisiopatologia
Bolsa Periodontal/microbiologia
Fosfolipases/análise
Reação em Cadeia da Polimerase
Radiografia Dentária
Estatísticas não Paramétricas
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); EC 3.1.- (Phospholipases); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170706
[St] Status:MEDLINE


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[PMID]:28538740
[Au] Autor:González-Jaranay M; Téllez L; Roa-López A; Gómez-Moreno G; Moreu G
[Ad] Endereço:Department of Periodontology, Faculty of Dentistry, Granada University, Granada, Spain.
[Ti] Título:Periodontal status during pregnancy and postpartum.
[So] Source:PLoS One;12(5):e0178234, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: Different studies have documented an association between periodontal disease and low birth-weight delivery. Hence, knowledge of periodontal status during pregnancy and postpartum is important in order to reduce the risks of both diseases. This study aimed to analyze periodontal status at successive stages of pregnancy and 3-6 weeks postpartum in women with initial periodontal alterations. MATERIALS AND METHODS: Ninety-six pregnant women were examined at 8-10 weeks (pregnancy diagnosis, baseline), 21-23 weeks and 34-36 weeks of gestation and at 40 days postpartum to record plaque scores, clinically assessed gingival inflammation and probing depth (mean depth and % sites with depth >3 mm). Bivariate and multivariate analyses were performed. Type 1 (α) error was established at 0.05. RESULTS: Plaque Index increased (p = 0.043) throughout pregnancy (baseline, 42%±0.18); 21-23 weeks, 42.6%±0.14; 34-36 weeks, 45.6%±0.13 and decreased postpartum (44.8%±0-13). Gingival Index increased (p<0.001) throughout pregnancy (baseline, 56.7%±0.20; 21-23 weeks, 66.36%±0.17; 34-36 weeks, 74.5%±0.18) and decreased postpartum (59.3%±0.21). Probing Depth increased (p<0.001) throughout pregnancy (baseline, 2.51±0.05; 21-23 weeks, 2.63±0.053; 34-36 weeks 2.81±0.055) and decreased postpartum (2.54±0.049). Percentage of sites with Probing Depth >3 mm increased (p<0.001) throughout pregnancy (baseline, 17.6%±0.16; 21-23 weeks, 23.9%±0.17; 34-36 weeks, 31.1%±0.17) and decreased postpartum (21.2%±0.17) but remained significantly (p<0.02) higher than at baseline. CONCLUSION: Periodontal status deteriorates during gestation but improves postpartum.
[Mh] Termos MeSH primário: Doenças Periodontais/patologia
Período Pós-Parto/fisiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Índice de Placa Dentária
Feminino
Gengivite/complicações
Gengivite/patologia
Seres Humanos
Análise Multivariada
Doenças Periodontais/complicações
Índice Periodontal
Bolsa Periodontal/patologia
Gravidez
Complicações na Gravidez
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170525
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178234


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[PMID]:28474067
[Au] Autor:Chen MH; Yin YZ
[Ad] Endereço:Department of Periodontology, Shanghai Stomatological Hospital. Shanghai 200001, China. E-mail:cathychendt@163.com.
[Ti] Título:[Efficacy of subgingival scaling of the molars with conventional scalers in vitro].
[So] Source:Shanghai Kou Qiang Yi Xue;26(1):54-58, 2017 Feb.
[Is] ISSN:1006-7248
[Cp] País de publicação:China
[La] Idioma:chi
[Ab] Resumo:PURPOSE: To evaluate the efficacy of traditional manual scaling and piezoelectric and magnetostrictive ultrasonic scaling in regard to periodontal pocket depth and power setting. METHODS: Thirty extracted human molars were assigned to receive manual scaling or ultrasonic scaling (both on the full or half power setting) with probing depth of 5 and 7 mm for 2 min. The remained oil materials on the roots were calculated as supplementary data. The data were compared using SAS6.12 software package. RESULTS: The procedures of ultrasonic scaling resulted in reductions of the values than manual scaling in all groups. The data of the magnetostrictive group were significantly smaller than the piezoelectric group in 5 mm pocket depth (P<0.01) on full power setting, while the opposite in 7 mm pocket depth. There was no significant difference between piezoelectric and magnetostrictive scaling in both 5 mm (P=0.217) and 7 mm (P=0.574) pocket depth on half power setting. The data increased significantly from 5 to 7 mm pocket depth (P<0.01), but no significant influence on different power settings. CONCLUSIONS: The study indicates that ultrasonic scaling instruments are more effective than a hand curette and the scaling efficacy decreased in deeper periodontal pocket.The results reveal no significant difference between magnetostrictive and piezoelectric ultrasonic instruments whether on half or full power settings.
[Mh] Termos MeSH primário: Raspagem Dentária
Desenho de Equipamento
Terapia por Ultrassom
[Mh] Termos MeSH secundário: Instrumentos Odontológicos
Seres Humanos
Dente Molar
Bolsa Periodontal
Ultrassom
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170506
[St] Status:MEDLINE


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[PMID]:28341446
[Au] Autor:Yoshida K; Okamura H; Hiroshima Y; Abe K; Kido JI; Shinohara Y; Ozaki K
[Ad] Endereço:Department of Oral Healthcare Education, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto, Tokushima 770-8504, Japan. Electronic address: kaya@tokushima-u.ac.jp.
[Ti] Título:PKR induces the expression of NLRP3 by regulating the NF-κB pathway in Porphyromonas gingivalis-infected osteoblasts.
[So] Source:Exp Cell Res;354(1):57-64, 2017 May 01.
[Is] ISSN:1090-2422
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The double-stranded RNA-dependent kinase (PKR), which is activated by double stranded RNA, induces inflammation by regulating NF-κB signaling. The NLR family pyrin domain-containing 3 (NLRP3) inflammasome also modulates inflammation in response to infection. Porphyromonas gingivalis (P.gingivalis) is an oral bacterium which is implicated in the pathogenesis of periodontal diseases. We previously reported that PKR is a key modulator of bone metabolism and inflammation in the periodontal tissue. PKR was also reported to induce inflammation in response to microbes by regulating the NLRP3 inflammasome, suggesting that PKR could affect inflammation along with NLRP3 in periodontal diseases. In this study, we investigated the effects of PKR on NLRP3 expression and NF-κB activity in P. gingivalis infected osteoblasts. We first constructed a SNAP26b-tagged P.gingivalis (SNAP-P. g.) and traced its internalization into the cell. SNAP-P. g. increased the activity of PKR and NF-κB and also induced NLRP3 expression in osteoblasts. Inhibition of NF-κB attenuated SNAP-P. g.-induced NLRP3 expression. The knockdown of PKR using shRNA decreased both the activity of NF-κB and the expression of NLRP3 induced by SNAP-P.g.. We therefore concluded that in osteoblasts, P. gingivalis activated PKR, which in turn increased NLRP3 expression by activating NF-κB. Our results suggest that PKR modulates inflammation by regulating the expression of the NLRP3 inflammasome through the NF-κB pathway in periodontal diseases.
[Mh] Termos MeSH primário: Inflamassomos/genética
Inflamação/genética
Proteína 3 que Contém Domínio de Pirina da Família NLR/genética
Receptores Acoplados a Proteínas-G/genética
Fator de Transcrição RelA/biossíntese
[Mh] Termos MeSH secundário: Células 3T3
Animais
Regulação da Expressão Gênica/genética
Seres Humanos
Inflamação/microbiologia
Inflamação/patologia
Camundongos
NF-kappa B/genética
Proteína 3 que Contém Domínio de Pirina da Família NLR/biossíntese
Osteoblastos/metabolismo
Osteoblastos/microbiologia
Osteoblastos/patologia
Bolsa Periodontal/genética
Bolsa Periodontal/microbiologia
Porphyromonas gingivalis/genética
Porphyromonas gingivalis/patogenicidade
Receptores Acoplados a Proteínas-G/biossíntese
Transdução de Sinais/genética
Fator de Transcrição RelA/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Inflammasomes); 0 (NF-kappa B); 0 (NLR Family, Pyrin Domain-Containing 3 Protein); 0 (Nlrp3 protein, mouse); 0 (PKR1 protein, mouse); 0 (Receptors, G-Protein-Coupled); 0 (Rela protein, mouse); 0 (Transcription Factor RelA)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170519
[Lr] Data última revisão:
170519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170326
[St] Status:MEDLINE


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[PMID]:28337902
[Au] Autor:Azzi L; Carinci F; Gabaglio S; Cura F; Croveri F; Tettamanti L; Tagliabue A; Segato S
[Ad] Endereço:Department of Medicine and Surgery, University of Insubria, ASST dei Sette Laghi, Dental Clinic, Unit of Oral Pathology, Varese, Italy.
[Ti] Título:Helicobacter pylori in periodontal pockets and saliva: a possible role in gastric infection relapses.
[So] Source:J Biol Regul Homeost Agents;31(1):257-262, 2017 Jan-Mar.
[Is] ISSN:0393-974X
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:It has been a long time since the scientific community started to speculate upon the presence of Helicobacter pylori (HP) in periodontal pockets as an extra-gastric reservoir responsible for gastric relapses after eradication therapy. The aim of this study is to evaluate the presence of oral HP in a group of patients who underwent examination for gastric infection. Sixty patients were enrolled in the current study, subdivided into two groups: 30 patients with a positive result for HP gastric infection with C-Breath Test Urea examination, and 30 patients with a negative result for HP gastric infection. Crevicular fluid and salivary samples were collected in a sterile tube and then sent to the laboratory for evaluation. Specimens were processed to quantify the levels of HP and bacterial load by real time PCR technique. Even though there was no statistically significant difference among the two groups (A vs B) with regard to the total amount of HP in saliva or in periodontal tissues, this study demonstrates that the oral cavity is an extra-gastric reservoir of HP when it is affected by periodontal disease, and that periodontal disease is correlated to gastric HP infection.
[Mh] Termos MeSH primário: Periodontite Crônica/microbiologia
Infecções por Helicobacter/microbiologia
Helicobacter pylori/patogenicidade
Bolsa Periodontal/microbiologia
Saliva/microbiologia
Úlcera Gástrica/microbiologia
Estômago/microbiologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Translocação Bacteriana
Testes Respiratórios
Estudos de Casos e Controles
Periodontite Crônica/diagnóstico
Periodontite Crônica/patologia
Reservatórios de Doenças/microbiologia
Feminino
Infecções por Helicobacter/diagnóstico
Infecções por Helicobacter/patologia
Helicobacter pylori/fisiologia
Seres Humanos
Masculino
Meia-Idade
Bolsa Periodontal/diagnóstico
Bolsa Periodontal/patologia
Recidiva
Estômago/patologia
Úlcera Gástrica/diagnóstico
Úlcera Gástrica/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170325
[St] Status:MEDLINE


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[PMID]:28325127
[Au] Autor:Pretzl B; Paul J; Krigar DM; Uhlmann L; Eickholz P; Dannewitz B
[Ad] Endereço:a Section of Periodontology, Department of Conservative Dentistry Clinic for Oral, Dental and Maxillofacial Diseases , University Hospital Heidelberg , Heidelberg , Germany.
[Ti] Título:Reproducibility of a commercially available subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes.
[So] Source:Acta Odontol Scand;75(4):302-307, 2017 May.
[Is] ISSN:1502-3850
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The aim was to evaluate the intra-test agreement of pooled samples from the deepest periodontal pocket of each quadrant with a commercially available test kit based on hybridization of 16S rRNA. MATERIAL AND METHODS: Plaque samples of 50 patients with generalized severe chronic periodontitis before therapy were pooled in two separate vials in order to detect and compare counts of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. Cohen's κ and interclass correlation coefficients were calculated to judge intra-test agreement. RESULTS: Cohen's κ for detection and counts of Tannerella forsythia and Treponema denticola showed a perfect agreement. Porphyromonas ginigivalis was identified in both tests with a substantial agreement, whereas detection of Aggregatibacter actinomycetemcomitans varied in eight patients resulting in a good agreement. Possible confounding factors could not be identified statistically. CONCLUSION: Test results of the commercial 16S rRNA test are perfectly reproducible regarding detection of red complex pathogens. Intra-test agreement concerning detection of Aggregatibacter actinomycetemcomitans was less favorable. CLINICAL RELEVANCE: Detection of certain periodontal pathogens may alter the treatment and lead to prescription of antibiotics parallel to mechanical debridement. It is quite important not to use antibiotics excessively. Thus, the basis for decision-making in favor of antibiotics should be solid.
[Mh] Termos MeSH primário: Carga Bacteriana/classificação
Periodontite Crônica/microbiologia
Placa Dentária/microbiologia
Bactérias Gram-Negativas/isolamento & purificação
Sondas de Oligonucleotídeos
Bolsa Periodontal/microbiologia
[Mh] Termos MeSH secundário: Seres Humanos
Bolsa Periodontal/classificação
Porphyromonas gingivalis/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oligonucleotide Probes)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170629
[Lr] Data última revisão:
170629
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170323
[St] Status:MEDLINE
[do] DOI:10.1080/00016357.2017.1303192


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[PMID]:28320314
[Au] Autor:Szafranski SP; Deng ZL; Tomasch J; Jarek M; Bhuju S; Rohde M; Sztajer H; Wagner-Döbler I
[Ad] Endereço:Microbial Communication, Helmholtz-Center for Infection Research, Braunschweig, Germany.
[Ti] Título:Quorum sensing of Streptococcus mutans is activated by Aggregatibacter actinomycetemcomitans and by the periodontal microbiome.
[So] Source:BMC Genomics;18(1):238, 2017 03 20.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The oral cavity is inhabited by complex microbial communities forming biofilms that can cause caries and periodontitis. Cell-cell communication might play an important role in modulating the physiologies of individual species, but evidence so far is limited. RESULTS: Here we demonstrate that a pathogen of the oral cavity, Aggregatibacter actinomycetemcomitans (A. act.), triggers expression of the quorum sensing (QS) regulon of Streptococcus mutans, a well-studied model organism for cariogenic streptococci, in dual-species biofilms grown on artificial saliva. The gene for the synthesis of the QS signal XIP is essential for this interaction. Transcriptome sequencing of biofilms revealed that S. mutans up-regulated the complete QS regulon (transformasome and mutacins) in the presence of A. act. and down-regulated oxidative stress related genes. A.act. required the presence of S. mutans for growth. Fimbriae and toxins were its most highly expressed genes and up-regulation of anaerobic metabolism, chaperones and iron acquisition genes was observed in co-culture. Metatranscriptomes from periodontal pockets showed highly variable levels of S. mutans and low levels of A. act.. Transcripts of the alternative sigma-factor SigX, the key regulator of QS in S. mutans, were significantly enriched in periodontal pockets compared to single cultures (log 4.159, FDR ≤0.001, and expression of mutacin related genes and transformasome components could be detected. CONCLUSION: The data show that the complete QS regulon of S. mutans can be induced by an unrelated oral pathogen and S. mutans may be competent in oral biofilms in vivo.
[Mh] Termos MeSH primário: Aggregatibacter actinomycetemcomitans/fisiologia
Interações Microbianas
Microbiota
Periodonto/microbiologia
Percepção de Quorum
Streptococcus mutans/fisiologia
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Proteínas de Bactérias/metabolismo
Biofilmes
Análise por Conglomerados
Perfilação da Expressão Gênica
Regulação Bacteriana da Expressão Gênica
Bolsa Periodontal/microbiologia
Fator sigma/genética
Fator sigma/metabolismo
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Sigma Factor)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171120
[Lr] Data última revisão:
171120
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170322
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-017-3618-5


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[PMID]:28232966
[Au] Autor:Rangbulla V; Nirola A; Gupta M; Batra P; Gupta M
[Ti] Título:Salivary IgA, Interleukin-1ß and MMP-8 as Salivary Biomarkers in Chronic Periodontitis Patients.
[So] Source:Chin J Dent Res;20(1):43-51, 2017.
[Is] ISSN:1462-6446
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To compare the clinical parameters and levels of salivary immunoglobulin A (IgA), Interleukin-1ß (IL-1ß) and matrix metalloproteinase-8 (MMP-8) in patients with moderate to severe chronic periodontitis and in individuals with healthy gingiva. METHODS: Levels of clinical parameters plaque index (PI), gingival index (GI), probing depth (PD), clinical attachment level (CAL) and bleeding on probing (BOP) along with salivary biomarkers salivary IgA, IL-1ß and MMP-8 were recorded among 50 adults (30 test subjects with moderate to severe generalised chronic periodontitis, constituting group A, and 20 periodontally healthy controls - group B). Clinical evaluation was done before oral prophylaxis, and 6 weeks and 12 weeks after oral prophylaxis, and saliva samples were obtained before and 12 weeks after oral prophylaxis. Salivary IgA, IL-1ß and MMP-8 levels in saliva were assessed using enzyme-linked immunosorbent assay. RESULTS: In group A, there were highly significant differences in terms of PI, GI, PD, CAL and BOP before oral prophylaxis, and 6 weeks and 12 weeks after oral prophylaxis when compared at these intervals. Differences in their levels in group B were non-significant at such intervals except PI. Mean levels of salivary IgA, IL-1ß and MMP-8 in chronic periodontitis patients at baseline were significantly higher than in the periodontally healthy group. Their levels in group A decreased significantly 12 weeks after oral prophylaxis, but remained static in group B. CONCLUSION: The levels of salivary IgA, IL-1ß and MMP-8 showed significant reduction after oral prophylaxis, suggesting that these biomarkers could facilitate the screening, early diagnosis, and management of periodontal disease.
[Mh] Termos MeSH primário: Periodontite Crônica/metabolismo
Imunoglobulina A/metabolismo
Interleucina-1beta/metabolismo
Metaloproteinase 8 da Matriz/metabolismo
Saliva/metabolismo
[Mh] Termos MeSH secundário: Adolescente
Adulto
Biomarcadores/metabolismo
Estudos de Casos e Controles
Índice de Placa Dentária
Seres Humanos
Meia-Idade
Perda da Inserção Periodontal
Índice Periodontal
Bolsa Periodontal
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Immunoglobulin A); 0 (Interleukin-1beta); EC 3.4.24.34 (Matrix Metalloproteinase 8)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170727
[Lr] Data última revisão:
170727
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170225
[St] Status:MEDLINE
[do] DOI:10.3290/j.cjdr.a37741



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