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[PMID]:28744587
[Au] Autor:Nakamura S; Ando M; Hamaguchi HO; Yamamoto M
[Ad] Endereço:Department of Periodontology, Showa University School of Dentistry, 2-1-1, Kitasenzoku, Ohta-ku, Tokyo, 145-8515, Japan.
[Ti] Título:Analysis of root surface properties by fluorescence/Raman intensity ratio.
[So] Source:Lasers Med Sci;32(8):1857-1863, 2017 Nov.
[Is] ISSN:1435-604X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study is to evaluate the existence of residual calculus on root surfaces by determining the fluorescence/Raman intensity ratio. Thirty-two extracted human teeth, partially covered with calculus on the root surface, were evaluated by using a portable Raman spectrophotometer, and a 785-nm, 100-mW laser was applied for fluorescence/Raman excitation. The collected spectra were normalized to the hydroxyapatite Raman band intensity at 960 cm . Raman spectra were recorded from the same point after changing the focal distance of the laser and the target radiating angle. In seven teeth, the condition of calculus, cementum, and dentin were evaluated. In 25 teeth, we determined the fluorescence/Raman intensity ratio following three strokes of debridement. Raman spectra collected from the dentin, cementum, and calculus were different. After normalization, spectra values were constant. The fluorescence/Raman intensity ratio of calculus region showed significant differences compared to the cementum and dentin (p < 0.05). The fluorescence/Raman intensity ratio decreased with calculus debridement. For this analysis, the delta value was defined as the difference between the values before and after three strokes, with the final 2 delta values close to zero, indicating a gradual asymptotic curve and the change in intensity ratio approximating that of individual constants. Fluorescence/Raman intensity ratio was effectively used to cancel the angle- and distance-dependent fluctuations of fluorescence collection efficiency during measurement. Changes in the fluorescence/Raman intensity ratio near zero suggested that cementum or dentin was exposed, and calculus removed.
[Mh] Termos MeSH primário: Cálculos Dentários/diagnóstico
Análise Espectral Raman/métodos
Raiz Dentária/patologia
[Mh] Termos MeSH secundário: Desbridamento
Fluorescência
Seres Humanos
Propriedades de Superfície
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1007/s10103-017-2291-x


  2 / 2433 MEDLINE  
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[PMID]:28273061
[Au] Autor:Weyrich LS; Duchene S; Soubrier J; Arriola L; Llamas B; Breen J; Morris AG; Alt KW; Caramelli D; Dresely V; Farrell M; Farrer AG; Francken M; Gully N; Haak W; Hardy K; Harvati K; Held P; Holmes EC; Kaidonis J; Lalueza-Fox C; de la Rasilla M; Rosas A; Semal P; Soltysiak A; Townsend G; Usai D; Wahl J; Huson DH; Dobney K; Cooper A
[Ad] Endereço:Australian Centre for Ancient DNA, School of Biological Sciences and The Environment Institute, University of Adelaide, Adelaide, South Australia, Australia.
[Ti] Título:Neanderthal behaviour, diet, and disease inferred from ancient DNA in dental calculus.
[So] Source:Nature;544(7650):357-361, 2017 04 20.
[Is] ISSN:1476-4687
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recent genomic data have revealed multiple interactions between Neanderthals and modern humans, but there is currently little genetic evidence regarding Neanderthal behaviour, diet, or disease. Here we describe the shotgun-sequencing of ancient DNA from five specimens of Neanderthal calcified dental plaque (calculus) and the characterization of regional differences in Neanderthal ecology. At Spy cave, Belgium, Neanderthal diet was heavily meat based and included woolly rhinoceros and wild sheep (mouflon), characteristic of a steppe environment. In contrast, no meat was detected in the diet of Neanderthals from El Sidrón cave, Spain, and dietary components of mushrooms, pine nuts, and moss reflected forest gathering. Differences in diet were also linked to an overall shift in the oral bacterial community (microbiota) and suggested that meat consumption contributed to substantial variation within Neanderthal microbiota. Evidence for self-medication was detected in an El Sidrón Neanderthal with a dental abscess and a chronic gastrointestinal pathogen (Enterocytozoon bieneusi). Metagenomic data from this individual also contained a nearly complete genome of the archaeal commensal Methanobrevibacter oralis (10.2× depth of coverage)-the oldest draft microbial genome generated to date, at around 48,000 years old. DNA preserved within dental calculus represents a notable source of information about the behaviour and health of ancient hominin specimens, as well as a unique system that is useful for the study of long-term microbial evolution.
[Mh] Termos MeSH primário: DNA Antigo/análise
Cálculos Dentários/química
Dieta/história
Preferências Alimentares
Saúde/história
Homem de Neandertal/microbiologia
Homem de Neandertal/psicologia
[Mh] Termos MeSH secundário: Animais
Bélgica
Carnivoridade
Cavernas
Enterocytozoon/genética
Enterocytozoon/isolamento & purificação
Genoma Bacteriano/genética
História Antiga
Seres Humanos
Intestinos/microbiologia
Carne/história
Methanobrevibacter/genética
Methanobrevibacter/isolamento & purificação
Boca/microbiologia
Pan troglodytes/microbiologia
Penicillium/química
Perissodáctilos
Ovinos
Espanha
Estômago/microbiologia
Simbiose
Fatores de Tempo
Vegetarianos/história
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Ancient)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170706
[Lr] Data última revisão:
170706
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1038/nature21674


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[PMID]:28105717
[Au] Autor:Radini A; Nikita E; Buckley S; Copeland L; Hardy K
[Ad] Endereço:BioArCh University of York, York, UK.
[Ti] Título:Beyond food: The multiple pathways for inclusion of materials into ancient dental calculus.
[So] Source:Am J Phys Anthropol;162 Suppl 63:71-83, 2017 Jan.
[Is] ISSN:1096-8644
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dental calculus (mineralized dental plaque) was first recognised as a potentially useful archaeological deposit in the 1970s, though interest in human dental calculus as a resource material has increased sharply in the past few years. The majority of recent research has focused on the retrieval of plant microfossils embedded in its matrix and interpretation of these finds as largely the result of deliberate consumption of plant-derived food. However, while most of the material described in published works does represent food, dental calculus is in fact a "depositional environment" as material can enter the mouth from a range of sources. In this respect, it therefore represents an archaeological deposit that can also contain extensive non-dietary debris. This can comprise a wide variety of cultural and environmental material which reaches the mouth and can become embedded in dental calculus through alternative pathways. Here, we explore the human behaviors and activities besides eating that can generate a flux of particles into the human mouth, the broad range of additional cultural and environmental information that can be obtained through the analysis and contextualisation of this material, and the implications of the additional pathways by which material can become embedded in dental calculus.
[Mh] Termos MeSH primário: Cálculos Dentários
Dieta/história
Alimentos/história
[Mh] Termos MeSH secundário: Arqueologia
Meio Ambiente
Fósseis
História Antiga
Seres Humanos
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE
[do] DOI:10.1002/ajpa.23147


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[PMID]:27981368
[Au] Autor:Hardy K; Radini A; Buckley S; Blasco R; Copeland L; Burjachs F; Girbal J; Yll R; Carbonell E; Bermúdez de Castro JM
[Ad] Endereço:ICREA, Pg. Lluís Companys 23, 08010, Barcelona, Catalonia, Spain. khardy@icrea.cat.
[Ti] Título:Diet and environment 1.2 million years ago revealed through analysis of dental calculus from Europe's oldest hominin at Sima del Elefante, Spain.
[So] Source:Naturwissenschaften;104(1-2):2, 2017 Feb.
[Is] ISSN:1432-1904
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Sima del Elefante, Atapuerca, Spain contains one of the earliest hominin fragments yet known in Europe, dating to 1.2 Ma. Dental calculus from a hominin molar was removed, degraded and analysed to recover entrapped remains. Evidence for plant use at this time is very limited and this study has revealed the earliest direct evidence for foods consumed in the genus Homo. This comprises starchy carbohydrates from two plants, including a species of grass from the Triticeae or Bromideae tribe, meat and plant fibres. All food was eaten raw, and there is no evidence for processing of the starch granules which are intact and undamaged. Additional biographical detail includes fragments of non-edible wood found adjacent to an interproximal groove suggesting oral hygiene activities, while plant fibres may be linked to raw material processing. Environmental evidence comprises spores, insect fragments and conifer pollen grains which are consistent with a forested environment.
[Mh] Termos MeSH primário: Cálculos Dentários/química
Dieta
Meio Ambiente
Fósseis
Hominidae/fisiologia
[Mh] Termos MeSH secundário: Animais
Comportamento Alimentar
Espanha
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170916
[Lr] Data última revisão:
170916
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


  5 / 2433 MEDLINE  
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Texto completo SciELO Brasil
[PMID]:28001239
[Au] Autor:Dogan GE; Demir T; Laloglu E; Saglam E; Aksoy H; Yildirim A; Akçay F
[Ad] Endereço:Private practice, Erzurum, Turkey.
[Ti] Título:Patients with dental calculus have increased saliva and gingival crevicular fluid fetuin-A levels but no association with fetuin-A polymorphisms.
[So] Source:Braz Oral Res;30(1):e129, 2016 Dec 22.
[Is] ISSN:1807-3107
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Fetuin-A is a potent inhibitor of calcium-phosphate precipitation and of the calcification process, therefore it can also be related with dental calculus. Thus, we aimed to investigate a possible relationship between fetuin-A gene polymorphism and the presence of dental calculus. A possible relationship between serum, saliva and gingival crevicular fluid (GCF) levels of fetuin-A was also investigated. Fetuin-A c.742C > T and c.766C > G polymorphisms were investigated in 103 patients with or without dental calculus. Additionally, serum, saliva and GCF fetuin-A levels of patients were compared according to dental calculus presence. A significant difference was not observed in the distribution of the fetuin-A c.742C > T and c.766C > G polymorphisms between patients with or without dental calculus. Saliva and GCF fetuin-A concentrations of patients with dental calculus were statistically higher than those without dental calculus (P=0.001, P=0.036 respectively). According to our results, fetuin-A c.742C > T and c.766C > G polymorphisms were not associated with presence of dental calculus. However, higher GCF and saliva fetuin-A levels were detected in patients with dental calculus than in patients without dental calculus, which may result from an adaptive mechanism to inhibit mineral precipitation and eventually calculus formation.
[Mh] Termos MeSH primário: Cálculos Dentários/química
Líquido do Sulco Gengival/química
Polimorfismo Genético
Saliva/química
alfa-2-Glicoproteína-HS/análise
alfa-2-Glicoproteína-HS/genética
[Mh] Termos MeSH secundário: Adulto
Análise de Variância
Estudos de Casos e Controles
Cálculos Dentários/genética
Cálculos Dentários/fisiopatologia
Placa Dentária/química
Ensaio de Imunoadsorção Enzimática
Feminino
Estudos de Associação Genética
Genótipo
Líquido do Sulco Gengival/fisiologia
Seres Humanos
Masculino
Meia-Idade
Valores de Referência
Saliva/fisiologia
Estatísticas não Paramétricas
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (alpha-2-HS-Glycoprotein)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:161222
[St] Status:MEDLINE


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[PMID]:27805890
[Au] Autor:Rubin PF; Winocur E; Erez A; Birenboim-Wilensky R; Peretz B
[Ti] Título:Dental Treatment Needs among Children and Adolescents Residing in an Ugandan Orphanage.
[So] Source:J Clin Pediatr Dent;40(6):486-489, 2016.
[Is] ISSN:1053-4628
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Previous studies focused on the dental caries status of East African children and not on their overall dental needs. Urban children consume more sugar-rich foods. OBJECTIVES: To assess overall dental treatment needs of children living in an orphanage in Uganda. STUDY DESIGN: Teeth were diagnosed as needing treatment by obvious frank carious lesions (WHO criteria), temporary fillings, staining, or very deep pit and/or fissures possibly requiring sealants. Calculus or crowding in the mandibular anterior region and evidence of tooth fractures were recorded, as were signs of wear on the mandibular molars and canines and the maxillary incisors. RESULTS: Most of the primary teeth (64%) required no dental treatment, but almost all (98%) of the permanent teeth did. A mean (±standard deviation) of 4.81±1.92 permanent teeth required treatment. The mean number of missing teeth was 0.47. Thirty-one children (20.2%) had crowding, 52 (34%) had calculus, and 49 (32%) had signs of attrition on primary and permanent molar teeth (45 enamel only and 4 enamel and dentin). CONCLUSIONS: Most of the primary teeth required no dental treatment, while the vast majority of permanent teeth did, possibly in association with high sugar cane consumption and poor brushing habits among older children.
[Mh] Termos MeSH primário: Crianças Órfãs
Assistência Odontológica
Determinação de Necessidades de Cuidados de Saúde
[Mh] Termos MeSH secundário: Adolescente
Criança
Pré-Escolar
Dente Canino/patologia
Cálculos Dentários/diagnóstico
Cárie Dentária/diagnóstico
Fissuras Dentárias/diagnóstico
Comportamento Alimentar
Feminino
Seres Humanos
Incisivo/patologia
Masculino
Má Oclusão/diagnóstico
Dente Molar/patologia
Selantes de Fossas e Fissuras/uso terapêutico
Descoloração de Dente/diagnóstico
Fraturas dos Dentes/diagnóstico
Perda de Dente/diagnóstico
Desgaste dos Dentes/diagnóstico
Dente Decíduo/patologia
Uganda
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pit and Fissure Sealants)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170125
[Lr] Data última revisão:
170125
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:161103
[St] Status:MEDLINE


  7 / 2433 MEDLINE  
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[PMID]:27632566
[Au] Autor:Montenegro Raudales JL; Yoshimura A; Sm Z; Kaneko T; Ozaki Y; Ukai T; Miyazaki T; Latz E; Hara Y
[Ad] Endereço:Department of Periodontology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
[Ti] Título:Dental Calculus Stimulates Interleukin-1ß Secretion by Activating NLRP3 Inflammasome in Human and Mouse Phagocytes.
[So] Source:PLoS One;11(9):e0162865, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Dental calculus is a mineralized deposit associated with periodontitis. The bacterial components contained in dental calculus can be recognized by host immune sensors, such as Toll-like receptors (TLRs), and induce transcription of proinflammatory cytokines, such as IL-1ß. Studies have shown that cellular uptake of crystalline particles may trigger NLRP3 inflammasome activation, leading to the cleavage of the IL-1ß precursor to its mature form. Phagocytosis of dental calculus in the periodontal pocket may therefore lead to the secretion of IL-1ß, promoting inflammatory responses in periodontal tissues. However, the capacity of dental calculus to induce IL-1ß secretion in human phagocytes has not been explored. To study this, we stimulated human polymorphonuclear leukocytes (PMNs) and peripheral blood mononuclear cells (PBMCs) with dental calculus collected from periodontitis patients, and measured IL-1ß secretion by ELISA. We found that calculus induced IL-1ß secretion in both human PMNs and PBMCs. Calculus also induced IL-1ß in macrophages from wild-type mice, but not in macrophages from NLRP3- and ASC-deficient mice, indicating the involvement of NLRP3 and ASC. IL-1ß induction was inhibited by polymyxin B, suggesting that LPS is one of the components of calculus that induces pro-IL-1ß transcription. To analyze the effect of the inorganic structure, we baked calculus at 250°C for 1 h. This baked calculus failed to induce pro-IL-1ß transcription. However, it did induce IL-1ß secretion in lipid A-primed cells, indicating that the crystalline structure of calculus induces inflammasome activation. Furthermore, hydroxyapatite crystals, a component of dental calculus, induced IL-1ß in mouse macrophages, and baked calculus induced IL-1ß in lipid A-primed human PMNs and PBMCs. These results indicate that dental calculus stimulates IL-1ß secretion via NLRP3 inflammasome in human and mouse phagocytes, and that the crystalline structure has a partial role in the activation of NLRP3 inflammasome.
[Mh] Termos MeSH primário: Cálculos Dentários/fisiopatologia
Inflamassomos/metabolismo
Interleucina-1beta/secreção
Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo
Fagócitos/metabolismo
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Camundongos
Fagócitos/secreção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Inflammasomes); 0 (Interleukin-1beta); 0 (NLR Family, Pyrin Domain-Containing 3 Protein); 0 (NLRP3 protein, human); 0 (Nlrp3 protein, mouse)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170807
[Lr] Data última revisão:
170807
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160916
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0162865


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[PMID]:27524540
[Au] Autor:Fatima T; Haji Abdul Rahim ZB; Lin CW; Qamar Z
[Ad] Endereço:Department of Oral Biology and Biomedical Sciences, Malaysia.
[Ti] Título:Zinc: A precious trace element for oral health care?
[So] Source:J Pak Med Assoc;66(8):1019-23, 2016 Aug.
[Is] ISSN:0030-9982
[Cp] País de publicação:Pakistan
[La] Idioma:eng
[Ab] Resumo:This review will discuss the importance of Zinc in the maintenance of oral health. Zinc (Zn) is a trace element of valuable importance. In the oral cavity, it is naturally present at various sites such as dental plaque, dental hard tissues and saliva. It is proven to be effective against common prevalent oral health problems such as dental caries, gingivitis, periodontitis and malodour. It is being used in various oral health care products to control the formation of dental plaque and inhibiting the formation of dental calculus. It has the potential to sustain and maintain its elevated concentrations for a longer time particularly in the dental plaque and saliva on delivery from the mouth rinses and toothpastes. It has been reported that low concentrations of zinc have the capability to reduce dissolution and promote remineralization under caries simulating conditions. Most importantly low Zn2+ levels in the serum are useful as a tumour marker. Thus taking a note of its potentials, it can be concluded that zinc is a precious element for the maintenance of oral health.
[Mh] Termos MeSH primário: Saúde Bucal
Zinco/fisiologia
[Mh] Termos MeSH secundário: Anti-Infecciosos Locais/uso terapêutico
Carcinoma de Células Escamosas/sangue
Cálculos Dentários/prevenção & controle
Cárie Dentária/tratamento farmacológico
Esmalte Dentário/química
Placa Dentária/química
Placa Dentária/tratamento farmacológico
Dentina/química
Gengivite/tratamento farmacológico
Neoplasias de Cabeça e Pescoço/sangue
Seres Humanos
Doenças da Boca/tratamento farmacológico
Neoplasias Bucais/sangue
Antissépticos Bucais/uso terapêutico
Saliva/química
Odontopatias/tratamento farmacológico
Cremes Dentais/uso terapêutico
Oligoelementos/uso terapêutico
Zinco/sangue
Zinco/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Infective Agents, Local); 0 (Mouthwashes); 0 (Toothpastes); 0 (Trace Elements); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170113
[Lr] Data última revisão:
170113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160816
[St] Status:MEDLINE


  9 / 2433 MEDLINE  
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[PMID]:27514153
[Au] Autor:Warinner C
[Ti] Título:Dental Calculus and the Evolution of the Human Oral Microbiome.
[So] Source:J Calif Dent Assoc;44(7):411-20, 2016 Jul.
[Is] ISSN:1043-2256
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Characterizing the evolution of the oral microbiome is a challenging, but increasingly feasible, task. Recently, dental calculus has been shown to preserve ancient biomolecules from the oral microbiota, host tissues and diet for tens of thousands of years. As such, it provides a unique window into the ancestral oral microbiome. This article reviews recent advancements in ancient dental calculus research and emerging insights into the evolution and ecology of the human oral microbiome.
[Mh] Termos MeSH primário: Cálculos Dentários/microbiologia
Microbiota/fisiologia
[Mh] Termos MeSH secundário: Cálculos Dentários/história
Cárie Dentária/microbiologia
Fósseis/microbiologia
História Antiga
História Medieval
Seres Humanos
Boca/microbiologia
Paleodontologia
Doenças Periodontais/microbiologia
[Pt] Tipo de publicação:HISTORICAL ARTICLE; JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; REVIEW
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160812
[Lr] Data última revisão:
160812
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:160813
[St] Status:MEDLINE


  10 / 2433 MEDLINE  
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[PMID]:27514152
[Au] Autor:Dewhirst FE
[Ti] Título:The Oral Microbiome: Critical for Understanding Oral Health and Disease.
[So] Source:J Calif Dent Assoc;44(7):409-10, 2016 Jul.
[Is] ISSN:1043-2256
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Microbiota/fisiologia
Doenças da Boca/microbiologia
Boca/microbiologia
Saúde Bucal
[Mh] Termos MeSH secundário: Técnicas Bacteriológicas
Cálculos Dentários/microbiologia
Cárie Dentária/microbiologia
Seres Humanos
Interações Microbianas
Doenças Periodontais/microbiologia
[Pt] Tipo de publicação:INTRODUCTORY JOURNAL ARTICLE
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160812
[Lr] Data última revisão:
160812
[Sb] Subgrupo de revista:D
[Da] Data de entrada para processamento:160813
[St] Status:MEDLINE



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