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[PMID]:28461340
[Au] Autor:Fusco R; Gugliandolo E; Biundo F; Campolo M; Di Paola R; Cuzzocrea S
[Ad] Endereço:Department of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Messina, Italy.
[Ti] Título:Inhibition of inflammasome activation improves lung acute injury induced by carrageenan in a mouse model of pleurisy.
[So] Source:FASEB J;31(8):3497-3511, 2017 08.
[Is] ISSN:1530-6860
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The inflammasome NLRP3 is a molecular pathway activated by a wide range of cellular insults to elicit innate immune defenses through the activation of caspase-1 and the maturation of proinflammatory cytokines, such as IL-1ß and IL-18. The expression of NRLP3 is abnormally elevated in numerous human inflammatory diseases, including pulmonary diseases. An injection of carrageenan (CAR) into the pleural cavity triggered an acute inflammatory response, leading to tissue damage, inflammatory exudates, leukocyte infiltration, and increased myeloperoxidase activity. The aim of this study was to assess the effect of the inflammasome blocking agents BAY 11-7082 (30 mg/kg, i.p.) and Brilliant Blue G (BBG) (45.5 mg/kg, i.p.) in a mouse model of CAR-induced pleurisy. Treatment with BAY 11-7082 or BBG 1 h after CAR injection attenuated pulmonary membrane thickening and polymorphonuclear leukocyte infiltration, reduced NF-κB translocation in the nucleus, and inhibited the assembly of the NRLP3/ASC/caspase-1 complex. Treatment with BAY 11-7082 or BBG also down-regulated iNOS, nitrotyrosine, and poly-ADP-ribosyl polymerase expression and inhibited CAR-induced apoptosis. Our results demonstrate that treatment with inflammasome-blocking agents can significantly reduce the development of acute CAR-induced lung injury.-Fusco, R. Gugliandolo, E., Biundo, F., Campolo, M., Di Paola, R., Cuzzocrea, S. Inhibition of inflammasome activation improves lung acute injury induced by carrageenan in a mouse model of pleurisy.
[Mh] Termos MeSH primário: Lesão Pulmonar Aguda/induzido quimicamente
Carragenina/toxicidade
Regulação da Expressão Gênica/efeitos dos fármacos
Inflamassomos/metabolismo
Pleurisia/induzido quimicamente
[Mh] Termos MeSH secundário: Lesão Pulmonar Aguda/metabolismo
Lesão Pulmonar Aguda/prevenção & controle
Animais
Citocinas/genética
Citocinas/metabolismo
Inflamassomos/efeitos dos fármacos
Inflamassomos/genética
Masculino
Camundongos
Fator 2 Relacionado a NF-E2/genética
Fator 2 Relacionado a NF-E2/metabolismo
Proteína 3 que Contém Domínio de Pirina da Família NLR/antagonistas & inibidores
Proteína 3 que Contém Domínio de Pirina da Família NLR/genética
Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo
Nitrilos/farmacologia
Pleurisia/metabolismo
Pleurisia/prevenção & controle
Corantes de Rosanilina/farmacologia
Sulfonas/farmacologia
Superóxido Dismutase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3-(4-methylphenylsulfonyl)-2-propenenitrile); 0 (Cytokines); 0 (Inflammasomes); 0 (NF-E2-Related Factor 2); 0 (NLR Family, Pyrin Domain-Containing 3 Protein); 0 (Nfe2l2 protein, mouse); 0 (Nitriles); 0 (Rosaniline Dyes); 0 (Sulfones); 9000-07-1 (Carrageenan); EC 1.15.1.1 (Superoxide Dismutase); M1ZRX790SI (coomassie Brilliant Blue)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170503
[St] Status:MEDLINE
[do] DOI:10.1096/fj.201601349R


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Texto completo SciELO Chile
[PMID]:29189866
[Au] Autor:Radich R J; Álvarez Z C; Valenzuela R A; Castillo H F; Moraga V F; Castillo A J
[Ad] Endereço:Departamento de Cirugía, Universidad de Chile, Santiago, Chile.
[Ti] Título:[Role of surgery in Saprochaete capitata (S. capitata) sepsis in a patient with acute myeloid leukemia].
[Ti] Título:Rol de la cirugía en el manejo de la infección invasora por Saprochaete capitata..
[So] Source:Rev Med Chil;145(8):1067-1071, 2017 Aug.
[Is] ISSN:0717-6163
[Cp] País de publicação:Chile
[La] Idioma:spa
[Ab] Resumo:Saprochaete capitata (S. capitata) fungal sepsis is a severe condition with a clinical presentation that is similar to other yeast originated fungal sepsis. It is observed in patients with hematological malignancies such as acute myeloid leukemia and neutropenia. We report a 23 year old male presenting with cough, fever and malaise. A bone marrow biopsy led to the diagnosis of acute myeloid leukemia. During the first cycle of chemotherapy the patient presented fever: blood cultures were positive for Klebsiella pneumoniae. Despite antimicrobial treatment, fever persisted; a computed tomography showed a focal splenic lesion; a left exudative pleural effusion appeared. A Matrix Assisted Laser Desorption Ionization-Time of Flight mass spectrometry identified the presence of S. capitata. After multiple antifungal treatments and pleural cavity cleansing by means of videothoracoscopy and laparoscopic splenectomy, the infection resolved and the patient completed his chemotherapy.
[Mh] Termos MeSH primário: Dipodascus/isolamento & purificação
Fungemia/cirurgia
Leucemia Mieloide Aguda/microbiologia
[Mh] Termos MeSH secundário: Antifúngicos/uso terapêutico
Drenagem/métodos
Fungemia/tratamento farmacológico
Fungemia/patologia
Seres Humanos
Masculino
Pleurisia/microbiologia
Pleurisia/patologia
Esplenectomia/métodos
Esplenopatias/microbiologia
Esplenopatias/patologia
Esplenopatias/cirurgia
Resultado do Tratamento
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171218
[Lr] Data última revisão:
171218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171201
[St] Status:MEDLINE


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[PMID]:28858765
[Au] Autor:Gonzaga DTG; Ferreira LBG; Moreira Maramaldo Costa TE; von Ranke NL; Anastácio Furtado Pacheco P; Sposito Simões AP; Arruda JC; Dantas LP; de Freitas HR; de Melo Reis RA; Penido C; Bello ML; Castro HC; Rodrigues CR; Ferreira VF; Faria RX; da Silva FC
[Ad] Endereço:Fundação Oswaldo Cruz, Instituto de Tecnologia em Fármacos, Farmanguinhos - Fiocruz, Departamento de Síntese de Fármacos Manguinhos, CEP 21041-250, Rio de Janeiro, RJ, Brazil; Universidade Federal Fluminense, Departamento de Química Orgânica, Instituto de Química, Campus do Valonguinho, CEP 24020-15
[Ti] Título:1-Aryl-1H- and 2-aryl-2H-1,2,3-triazole derivatives blockade P2X7 receptor in vitro and inflammatory response in vivo.
[So] Source:Eur J Med Chem;139:698-717, 2017 Oct 20.
[Is] ISSN:1768-3254
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Fifty-one 1,2,3-triazole derivatives were synthesized and evaluated with respect to P2X7 receptor (P2X7R) activity and its associated pore. These triazoles were screened in vitro for dye uptake assay and its cytotoxicity against mammalian cell types. Seven 1,2,3-triazole derivatives (5e, 6e, 8h, 9d, 9i, 11, and 12) potently blocked P2X7 receptor pore formation in vitro (J774.G8 cells and peritoneal macrophages). All blockers displayed IC value inferior to 500 nM, and they have low toxicity in either cell types. These seven selected triazoles inhibited P2X7R mediated interleukin-1 (IL-1ß) release. In particular, compound 9d was the most potent P2X7R blocker. Additionally, in mouse acute models of inflammatory responses induced by ATP or carrageenan administration in the paw, compound 9d promoted a potent blocking response. Similarly, 9d also reduced mouse LPS-induced pleurisy cellularity. In silico predictions indicate this molecule appropriate to develop an anti-inflammatory agent when it was compared to commercial analogs. Electrophysiological studies suggest a competitive mechanism of action of 9d to block P2X7 receptor. Molecular docking was performed on the ATP binding site in order to observe the preferential interaction pose, indicating that binding mode of the 9d is by interacting its 1,2,3-triazole and ether moiety with positively charged residues and with its chlorobenzene moiety orientated toward the apolar end of the ATP binding site which are mainly composed by the Ile170, Trp167 and Leu309 residues from α subunit. These results highlight 9d derivative as a drug candidate with potential therapeutic application based on P2X7 receptor blockade.
[Mh] Termos MeSH primário: Inflamação/tratamento farmacológico
Pleurisia/tratamento farmacológico
Receptores Purinérgicos P2X7/metabolismo
Triazóis/farmacologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Relação Dose-Resposta a Droga
Inflamação/metabolismo
Lipopolissacarídeos/antagonistas & inibidores
Lipopolissacarídeos/farmacologia
Camundongos
Estrutura Molecular
Relação Estrutura-Atividade
Triazóis/síntese química
Triazóis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lipopolysaccharides); 0 (Receptors, Purinergic P2X7); 0 (Triazoles)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE


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[PMID]:28726658
[Au] Autor:Bayramov B
[Ad] Endereço:Scientific Research Institute Of Pulmonary Illness of Azerbaijan Health Ministry.
[Ti] Título:[OPTIMIZATION OF DIAGNOSTICS OF EXUDATIVE PLEURITIS OF UNKNOWN ORIGIN].
[So] Source:Georgian Med News;(267):72-76, 2017 Jun.
[Is] ISSN:1512-0112
[Cp] País de publicação:Georgia (Republic)
[La] Idioma:rus
[Ab] Resumo:The article describes 143 cases of exudative pleuritis of unknown origin in patients who were diagnosed and treated with any minimally invasive surgical procedures: pleural puncture (PP), video-assisted thoracoscopy (VATS) with biopsy of the pleura. A different diagnostic methods (cytological, microbiological, histological) used in various diagnostic surgical procedures are analyzed in detail and calculated diagnostic sensitivity, specificity, and accuracy of each method. Based on the analysis of cytological, histological and microbiological studies in the performance of VATS concluded relatively high, comparable with the method of open pleural biopsy, diagnostic efficiency, the sensitivity of this method in the scheme of the diagnostic algorithm of EP of unknown origin.
[Mh] Termos MeSH primário: Derrame Pleural/diagnóstico
Pleurisia/diagnóstico
[Mh] Termos MeSH secundário: Exsudatos e Transudatos
Feminino
Seres Humanos
Masculino
Derrame Pleural/etiologia
Derrame Pleural/microbiologia
Derrame Pleural Maligno/diagnóstico
Pleurisia/etiologia
Pleurisia/microbiologia
Tuberculose Pulmonar/complicações
Tuberculose Pulmonar/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE


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[PMID]:28690754
[Au] Autor:Daib A; Hellal Y; Boughdir M; Abdallah RB; Kaabar N
[Ad] Endereço:Département de Chirurgie Pédiatrique, Hôpital Habib Thameur, 1008 Tunis, Tunisia.
[Ti] Título:[Pancreatic-pleural fistula in children: a rare cause of great abundant pleurisy].
[Ti] Título:La fistule pancréatico-pleurale chez l'enfant: étiologie rare d'une pleurésie de grande abondance..
[So] Source:Pan Afr Med J;26:240, 2017.
[Is] ISSN:1937-8688
[Cp] País de publicação:Uganda
[La] Idioma:fre
[Ab] Resumo:Pancreatic-pleural fistula is a very rare complication of false cysts of the pancreas. Our study aimed to describe this rare pathology affecting children. We emphasized the importance of evoking this diagnosis in the presence of great abundant pleurisy even in the absence of the digestive signs. We here report the case of a 2-year old child with false cyst of the pancreas complicated by pleural fistula detected after great abundant pleurisy with no digestive signs associated.
[Mh] Termos MeSH primário: Fístula Pancreática/diagnóstico
Pseudocisto Pancreático/diagnóstico
Doenças Pleurais/diagnóstico
Pleurisia/etiologia
[Mh] Termos MeSH secundário: Pré-Escolar
Seres Humanos
Masculino
Fístula Pancreática/complicações
Fístula Pancreática/patologia
Pseudocisto Pancreático/patologia
Doenças Pleurais/complicações
Doenças Pleurais/patologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.11604/pamj.2017.26.240.9003


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[PMID]:28655761
[Au] Autor:Lima KM; Vago JP; Caux TR; Negreiros-Lima GL; Sugimoto MA; Tavares LP; Arribada RG; Carmo AAF; Galvão I; Costa BRC; Soriani FM; Pinho V; Solito E; Perretti M; Teixeira MM; Sousa LP
[Ad] Endereço:From the Programa de Pós-Graduação em Biologia Celular, Departamento de Morfologia, Instituto de Ciências Biológicas.
[Ti] Título:The resolution of acute inflammation induced by cyclic AMP is dependent on annexin A1.
[So] Source:J Biol Chem;292(33):13758-13773, 2017 Aug 18.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Annexin A1 (AnxA1) is a glucocorticoid-regulated protein known for its anti-inflammatory and pro-resolving effects. We have shown previously that the cAMP-enhancing compounds rolipram (ROL; a PDE4 inhibitor) and Bt cAMP (a cAMP mimetic) drive caspase-dependent resolution of neutrophilic inflammation. In this follow-up study, we investigated whether AnxA1 could be involved in the pro-resolving properties of these compounds using a model of LPS-induced inflammation in BALB/c mice. The treatment with ROL or Bt cAMP at the peak of inflammation shortened resolution intervals, improved resolution indices, and increased AnxA1 expression. studies showed that ROL and Bt cAMP induced AnxA1 expression and phosphorylation, and this effect was prevented by PKA inhibitors, suggesting the involvement of PKA in ROL-induced AnxA1 expression. Akin to these findings, H89 prevented ROL- and Bt cAMP-induced resolution of inflammation, and it was associated with decreased levels of intact AnxA1. Moreover, two different strategies to block the AnxA1 pathway (by using -Boc-Met-Leu-Phe, a nonselective AnxA1 receptor antagonist, or by using an anti-AnxA1 neutralizing antiserum) prevented ROL- and Bt cAMP-induced resolution and neutrophil apoptosis. Likewise, the ability of ROL or Bt cAMP to induce neutrophil apoptosis was impaired in AnxA-knock-out mice. Finally, in settings, ROL and Bt cAMP overrode the survival-inducing effect of LPS in human neutrophils in an AnxA1-dependent manner. Our results show that AnxA1 is at least one of the endogenous determinants mediating the pro-resolving properties of cAMP-elevating agents and cAMP-mimetic drugs.
[Mh] Termos MeSH primário: Anexina A1/agonistas
Bucladesina/uso terapêutico
AMP Cíclico/agonistas
Infiltração de Neutrófilos/efeitos dos fármacos
Inibidores da Fosfodiesterase 4/uso terapêutico
Pleurisia/tratamento farmacológico
Rolipram/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Anexina A1/antagonistas & inibidores
Anexina A1/genética
Anexina A1/metabolismo
Apoptose/efeitos dos fármacos
Bucladesina/antagonistas & inibidores
Células Cultivadas
AMP Cíclico/análogos & derivados
AMP Cíclico/antagonistas & inibidores
AMP Cíclico/metabolismo
Proteínas Quinases Dependentes de AMP Cíclico/antagonistas & inibidores
Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
Seres Humanos
Lipopolissacarídeos/toxicidade
Macrófagos/efeitos dos fármacos
Macrófagos/imunologia
Macrófagos/metabolismo
Macrófagos/patologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Neutrófilos/efeitos dos fármacos
Neutrófilos/imunologia
Neutrófilos/metabolismo
Neutrófilos/patologia
Inibidores da Fosfodiesterase 4/química
Fosforilação/efeitos dos fármacos
Pleurisia/imunologia
Pleurisia/metabolismo
Pleurisia/patologia
Inibidores de Proteínas Quinases/farmacologia
Processamento de Proteína Pós-Traducional/efeitos dos fármacos
Células RAW 264.7
Rolipram/antagonistas & inibidores
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Annexin A1); 0 (Lipopolysaccharides); 0 (Phosphodiesterase 4 Inhibitors); 0 (Protein Kinase Inhibitors); 0 (annexin A1, mouse); 63X7MBT2LQ (Bucladesine); E0399OZS9N (Cyclic AMP); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases); K676NL63N7 (Rolipram)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.800391


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[PMID]:28609232
[Au] Autor:Mathis G
[Ad] Endereço:1 Internistische Praxis, Rankweil, Österreich.
[Ti] Título:Solide Pleuraveränderungen im Ultraschall..
[So] Source:Praxis (Bern 1994);106(12):641-645, 2017 Jun.
[Is] ISSN:1661-8157
[Cp] País de publicação:Switzerland
[La] Idioma:ger
[Mh] Termos MeSH primário: Mesotelioma/diagnóstico por imagem
Derrame Pleural/diagnóstico por imagem
Neoplasias Pleurais/diagnóstico por imagem
Neoplasias Pleurais/secundário
Ultrassonografia/métodos
[Mh] Termos MeSH secundário: Diagnóstico Diferencial
Empiema Pleural/diagnóstico por imagem
Seres Humanos
Pleura/diagnóstico por imagem
Pleurisia/diagnóstico por imagem
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1024/1661-8157/a002719


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[PMID]:28480389
[Au] Autor:Paniagua-Pérez R; Flores-Mondragón G; Reyes-Legorreta C; Herrera-López B; Cervantes-Hernández I; Madrigal-Santillán O; Morales-González JA; Álvarez-González I; Madrigal-Bujaidar E
[Ad] Endereço:Instituto Nacional de Rehabilitación, Servicio de Bioquímica. Av. México-Xochimilco 289, Ciudad de México, 14389. MÉXICO.
[Ti] Título:EVALUATION OF THE ANTI-INFLAMMATORY CAPACITY OF BETA-SITOSTEROL IN RODENT ASSAYS.
[So] Source:Afr J Tradit Complement Altern Med;14(1):123-130, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Beta-sitosterol (BS) is a compound discovered to be present in numerous plants. A number of interesting biomedical properties have been attributed to BS, including immuno-modulating and anti-inflammatory activities. Therefore, the aim of this report was to evaluate its anti-inflammatory capacity by applying various rodent experimental tests. METHODS: To carry out the objective of the study we applied the methods indicated here. Two of the adopted methods were based on the passive reverse Arthus reaction: the rat paw edema test and the rat pleurisy assay. We also applied two methods related with the non-specific acute inflammation: the mouse ear edema test, and the mouse mieloperoxidase activity assay. RESULTS: The results obtained in all tests established a significant anti-inflammatory potential of BS. In the rat paw edema test we found an inhibitory effect which goes from 50-70%; in the rat pleurisy assay our findings with respect to the volume of pleural exuded showed a reduction of 46%, as well as a 20% low amount of neutrophils in comparison with the level of the control group. In the mouse ear edema test we found a mean inflammatory inhibition of 75%, and with respect to mieloproxidase activity the results showed a significant inhibition induced by the three doses of BS. CONCLUSIONS: In the present study we determined a potent anti-inflammatory capacity of BS in specific and non-specific types of acute inflammation in rodents.
[Mh] Termos MeSH primário: Anti-Inflamatórios/administração & dosagem
Edema/tratamento farmacológico
Extratos Vegetais/administração & dosagem
Pleurisia/tratamento farmacológico
Sitosteroides/administração & dosagem
[Mh] Termos MeSH secundário: Animais
Avaliação Pré-Clínica de Medicamentos
Edema/imunologia
Seres Humanos
Masculino
Camundongos
Pleurisia/imunologia
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Plant Extracts); 0 (Sitosterols); 5LI01C78DD (gamma-sitosterol)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i1.13


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[PMID]:28242648
[Au] Autor:Kaneva MK; Greco KV; Headland SE; Montero-Melendez T; Mori P; Greenslade K; Pitzalis C; Moore A; Perretti M
[Ad] Endereço:William Harvey Research Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London EC1M 6BQ, United Kingdom; and m.kaneva@qmul.ac.uk m.perretti@qmul.ac.uk.
[Ti] Título:Identification of Novel Chondroprotective Mediators in Resolving Inflammatory Exudates.
[So] Source:J Immunol;198(7):2876-2885, 2017 Apr 01.
[Is] ISSN:1550-6606
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We hypothesized that exudates collected at the beginning of the resolution phase of inflammation might be enriched for tissue protective molecules; thus an integrated cellular and molecular approach was applied to identify novel chondroprotective bioactions. Exudates were collected 6 h (inflammatory) and 24 h (resolving) following carrageenan-induced pleurisy in rats. The resolving exudate was subjected to gel filtration chromatography followed by proteomics, identifying 61 proteins. Fractions were added to C28/I2 chondrocytes, grown in micromasses, ions with or without IL-1ß or osteoarthritic synovial fluids for 48 h. Three proteins were selected from the proteomic analysis, α1-antitrypsin (AAT), hemopexin (HX), and gelsolin (GSN), and tested against catabolic stimulation for their effects on glycosaminoglycan deposition as assessed by Alcian blue staining, and gene expression of key anabolic proteins by real-time PCR. In an in vivo model of inflammatory arthritis, cartilage integrity was determined histologically 48 h after intra-articular injection of AAT or GSN. The resolving exudate displayed protective activities on chondrocytes, using multiple readouts: these effects were retained in low m.w. fractions of the exudate (46.7% increase in glycosaminoglycan deposition; ∼20% upregulation of COL2A1 and aggrecan mRNA expression), which reversed the effect of IL-1ß. Exogenous administration of HX, GSN, or AAT abrogated the effects of IL-1ß and osteoarthritic synovial fluids on anabolic gene expression and increased glycosaminoglycan deposition. Intra-articular injection of AAT or GSN protected cartilage integrity in mice with inflammatory arthritis. In summary, the strategy for identification of novel chondroprotective activities in resolving exudates identified HX, GSN and AAT as potential leads for new drug discovery programs.
[Mh] Termos MeSH primário: Artrite Experimental/patologia
Condrócitos/efeitos dos fármacos
Exsudatos e Transudatos/química
Pleurisia/imunologia
[Mh] Termos MeSH secundário: Animais
Modelos Animais de Doenças
Masculino
Espectrometria de Massas
Camundongos
Camundongos Endogâmicos C57BL
Osteoartrite do Joelho/patologia
Proteômica
Ratos
Ratos Wistar
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170301
[St] Status:MEDLINE
[do] DOI:10.4049/jimmunol.1601111


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[PMID]:28222179
[Au] Autor:Zuntini Viscardi D; Arrigo JD; Correia CA; Kassuya CA; Cardoso CA; Maldonade IR; Argandoña EJ
[Ad] Endereço:College of Exact and Technological Sciences, Federal University of Grande Dourados, Dourados, Mato Grosso do Sul, Brazil.
[Ti] Título:Seed and peel essential oils obtained from Campomanesia adamantium fruit inhibit inflammatory and pain parameters in rodents.
[So] Source:PLoS One;12(2):e0157107, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Campomanesia adamantium (Myrtaceae) is popularly known as "gabiroba" and has been used in folk medicine as antirheumatic, antidiarrheal, hypocholesterolemic and anti-inflammatory. This study evaluated the anti-inflammatory and antinociceptive activities and toxicology of essential oils from peel (EOP) and seed (EOS) of C. adamantium fruits in animal models. Different groups were treated with doses of 100 and 300 mg/kg and the inflammatory parameters were evaluated in carrageenan induced paw oedema and leukocyte migration in pleurisy model, while antinociceptive activity was evaluated using formalin method in rodents. The major constituent of EOP and EOS was limonene with 13.07% and 20.89%, respectively. No clinical signs of toxicity have been observed in animals. It was observed a significant decreased (P<0.01) in leukocyte migration at the dose of 300 mg/kg of EOP and EOS, with maximal inhibition of 89±3% for EOP and 80±6% for EOS. Paw oedema was inhibited at all times, and maximal inhibition was at the dose of 100 mg/kg at 2 h after carrageenan injection with 72±2% for EOP and 74±2% for EOS. EOS and EOP also reduced the first and second phases of formalin-induced nociception test. In the first formalin-phase, maximal inhibitions were at 48±5% for EOP and 66±4% for EOS (300 mg/kg). At the inflammatory phase induced by formalin, maximal inhibitions were 72±2% for EOP and 80±2% for EOS at the dose of 100 mg/kg. Seed and peel essential oils from C. adamantium fruit inhibited leukocyte migration, inflammatory and neurogenic pain and oedema suggesting their use as nutraceutical or pharmacological agent.
[Mh] Termos MeSH primário: Analgésicos/farmacologia
Anti-Inflamatórios/farmacologia
Óleos Voláteis/farmacologia
Pimenta/química
[Mh] Termos MeSH secundário: Analgésicos/isolamento & purificação
Animais
Anti-Inflamatórios/isolamento & purificação
Carragenina/toxicidade
Quimiotaxia de Leucócito/efeitos dos fármacos
Temperatura Baixa/efeitos adversos
Avaliação Pré-Clínica de Medicamentos
Edema/tratamento farmacológico
Feminino
Formaldeído/toxicidade
Frutas/química
Hiperalgesia/tratamento farmacológico
Hiperalgesia/etiologia
Masculino
Camundongos
Óleos Voláteis/isolamento & purificação
Óleos Voláteis/toxicidade
Dor/induzido quimicamente
Dor/tratamento farmacológico
Dor/etiologia
Plantas Medicinais/química
Pleurisia/tratamento farmacológico
Ratos
Ratos Wistar
Sementes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Analgesics); 0 (Anti-Inflammatory Agents); 0 (Oils, Volatile); 1HG84L3525 (Formaldehyde); 9000-07-1 (Carrageenan)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0157107



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