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[PMID]:28465077
[Au] Autor:Malet M; Leiguarda C; Gastón G; McCarthy C; Brumovsky P
[Ad] Endereço:Instituto de Investigaciones en Medicina Traslacional (IIMT), Consejo Nacional de Investigaciones Cientiíficas y Técnicas (CONICET) - Austral University, Avenida Juan D. Perón 1500, B1629AHJ, Pilar, Buenos Aires, Argentina.
[Ti] Título:Spinal activation of the NPY Y1 receptor reduces mechanical and cold allodynia in rats with chronic constriction injury.
[So] Source:Peptides;92:38-45, 2017 Jun.
[Is] ISSN:1873-5169
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Neuropeptide tyrosine (NPY) and its associated receptors Y1R and Y2R have been previously implicated in the spinal modulation of neuropathic pain induced by total or partial sectioning of the sciatic nerve. However, their role in chronic constrictive injuries of the sciatic nerve has not yet been described. In the present study, we analyzed the consequences of pharmacological activation of spinal Y1R, by using the specific Y1R agonist Leu Pro -NPY, in rats with chronic constriction injury (CCI). CCI and sham-injury rats were implanted with a permanent intrathecal catheter (at day 7 after injury), and their response to the administration of different doses (2.5, 5, 7, 10 or 20µg) of Leu Pro -NPY (at a volume of 10µl) through the implanted catheter, recorded 14days after injury. Mechanical allodynia was tested by means of the up-and-down method, using von Frey filaments. Cold allodynia was tested by application of an acetone drop to the affected hindpaw. Intrathecal Leu Pro -NPY induced an increase of mechanical thresholds in rats with CCI, starting at doses of 5µg and becoming stronger with higher doses. Intrathecal Leu Pro also resulted in reductions in the frequency of withdrawal to cold stimuli, although the effect was somewhat more moderate and mostly observed for doses of 7µg and higher. We thus show that spinal activation of the Y1R is able to reduce neuropathic pain due to a chronic constrictive injury and, together with other studies, support the use of a spinal Y1R agonist as a therapeutic agent against chronic pain induced by peripheral neuropathy.
[Mh] Termos MeSH primário: Hiperalgesia/metabolismo
Neuropeptídeo Y/metabolismo
Receptores de Neuropeptídeo Y/metabolismo
Nervo Isquiático/lesões
Neuropatia Ciática/metabolismo
Medula Espinal/metabolismo
[Mh] Termos MeSH secundário: Análise de Variância
Animais
Dor Crônica/metabolismo
Temperatura Baixa
Constrição Patológica/complicações
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Hiperalgesia/etiologia
Injeções Espinhais
Masculino
Neuralgia/metabolismo
Medição da Dor
Limiar da Dor
Ratos
Ratos Sprague-Dawley
Receptores de Neuropeptídeo Y/agonistas
Neuropatia Ciática/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuropeptide Y); 0 (Receptors, Neuropeptide Y)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


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[PMID]:27771719
[Au] Autor:Lee H; Baek J; Min H; Cho IH; Yu SW; Lee SJ
[Ad] Endereço:Department of Neuroscience and Physiology, and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
[Ti] Título:Toll-Like Receptor 3 Contributes to Wallerian Degeneration after Peripheral Nerve Injury.
[So] Source:Neuroimmunomodulation;23(4):209-216, 2016.
[Is] ISSN:1423-0216
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: It is well known that Schwann cells play an important role in Wallerian degeneration after peripheral nerve injury. Previously, we reported that toll-like receptor 3 (TLR3) is expressed on Schwann cells, implicating its role in Schwann cell activation during Wallerian degeneration. In this study, we tested this possibility using TLR3 knock-out mice. METHODS: Sciatic nerve-crush injury was induced in wild-type and TLR3 knock-out mice. Histological sections of the sciatic nerve were analyzed for Wallerian degeneration on days 3 and 7 after injury. The level of macrophage infiltration was measured by real-time RT-PCR, flow cytometry and immunohistochemistry. The macrophage-recruiting chemokine gene expressions in the injured nerve were determined by real-time RT-PCR. RESULTS: In TLR3 knock-out mice, the nerve injury-induced axonal degeneration and subsequent axonal debris clearance were reduced compared to in wild-type mice. In addition, nerve injury-induced macrophage infiltration into injury sites was attenuated in TLR3 knock-out mice and was accompanied by reduced expression of macrophage-recruiting chemokines such as CC-chemokine ligands (CCL)2/MCP-1, CCL4/MIP-1ß and CCL5/RANTES. These macrophage-recruiting chemokines were induced in primary Schwann cells upon TLR3 stimulation. Finally, intraneural injection of polyinosinic-polycytidylic acid, a synthetic TLR3 agonist, induced macrophage infiltration into the sciatic nerve in vivo. CONCLUSION: These data show that TLR3 signaling contributes to Wallerian degeneration after peripheral nerve injury by affecting Schwann cell activation and macrophage recruitment to injured nerves.
[Mh] Termos MeSH primário: Neuropatia Ciática/metabolismo
Neuropatia Ciática/patologia
Receptor 3 Toll-Like/deficiência
Degeneração Walleriana/metabolismo
Degeneração Walleriana/patologia
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Knockout
Traumatismos dos Nervos Periféricos/metabolismo
Traumatismos dos Nervos Periféricos/patologia
Ratos
Ratos Sprague-Dawley
Células de Schwann/metabolismo
Células de Schwann/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (TLR3 protein, mouse); 0 (Toll-Like Receptor 3)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180213
[Lr] Data última revisão:
180213
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1159/000449134


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[PMID]:29304038
[Au] Autor:Madhankumar AB; Mrowczynski OD; Slagle-Webb B; Ravi V; Bourcier AJ; Payne R; Harbaugh KS; Rizk E; Connor JR
[Ad] Endereço:Department of Neurosurgery, Pennsylvania State University College of Medicine, Hershey, PA, United States of America.
[Ti] Título:Tumor targeted delivery of doxorubicin in malignant peripheral nerve sheath tumors.
[So] Source:PLoS One;13(1):e0181529, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Peripheral nerve sheath tumors are benign tumors that have the potential to transform into malignant peripheral nerve sheath tumors (MPNSTs). Interleukin-13 receptor alpha 2 (IL13Rα2) is a cancer associated receptor expressed in glioblastoma and other invasive cancers. We analyzed IL13Rα2 expression in several MPNST cell lines including the STS26T cell line, as well as in several peripheral nerve sheath tumors to utilize the IL13Rα2 receptor as a target for therapy. In our studies, we demonstrated the selective expression of IL13Rα2 in several peripheral nerve sheath tumors by immunohistochemistry (IHC) and immunoblots. We established a sciatic nerve MPNST mouse model in NIH III nude mice using a luciferase transfected STS26T MPNST cell line. Similarly, analysis of the mouse sciatic nerves after tumor induction revealed significant expression of IL13Rα2 by IHC when compared to a normal sciatic nerve. IL13 conjugated liposomal doxorubicin was formulated and shown to bind and internalized in the MPNST cell culture model demonstrating cytotoxic effect. Our subsequent in vivo investigation in the STS26T MPNST sciatic nerve tumor model indicated that IL13 conjugated liposomal doxorubicin (IL13LIPDXR) was more effective in inhibiting tumor progression compared to unconjugated liposomal doxorubicin (LIPDXR). This further supports that IL13 receptor targeted nanoliposomes is a potential approach for treating MPNSTs.
[Mh] Termos MeSH primário: Antibióticos Antineoplásicos/administração & dosagem
Doxorrubicina/análogos & derivados
Neoplasias da Bainha Neural/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antibióticos Antineoplásicos/farmacocinética
Linhagem Celular Tumoral
Doxorrubicina/administração & dosagem
Doxorrubicina/farmacocinética
Sistemas de Liberação de Medicamentos
Seres Humanos
Imuno-Histoquímica
Interleucina-13/administração & dosagem
Subunidade alfa2 de Receptor de Interleucina-13/metabolismo
Antígeno Ki-67/metabolismo
Camundongos
Camundongos Nus
Neoplasias da Bainha Neural/imunologia
Neoplasias da Bainha Neural/metabolismo
Polietilenoglicóis/administração & dosagem
Polietilenoglicóis/farmacocinética
Proteínas S100/metabolismo
Neuropatia Ciática/tratamento farmacológico
Neuropatia Ciática/imunologia
Neuropatia Ciática/metabolismo
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibiotics, Antineoplastic); 0 (Interleukin-13); 0 (Interleukin-13 Receptor alpha2 Subunit); 0 (Ki-67 Antigen); 0 (Mki67 protein, mouse); 0 (S100 Proteins); 0 (liposomal doxorubicin); 30IQX730WE (Polyethylene Glycols); 80168379AG (Doxorubicin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180129
[Lr] Data última revisão:
180129
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181529


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[PMID]:29182108
[Au] Autor:Baki ME; Abdioglu A; Aydin H; Kerimoglu S; Bak C
[Ti] Título:Triple pelvic osteotomy for the treatment of symptomatic acetabular dysplasia in adolescents and adults : A review of 42 hips.
[So] Source:Acta Orthop Belg;82(4):699-704, 2016 Dec.
[Is] ISSN:0001-6462
[Cp] País de publicação:Belgium
[La] Idioma:eng
[Ab] Resumo:We treated 42 hips with symptomatic acetabular dysplasia using triple pelvic osteotomy. The mean age of the patients was 20.7 years (12-47). The median follow-up was 50.3 months. The average Harris hip score improved from 74 to 92 points. Significant improvement from the preoperative to the latest follow-up evaluation was seen radiologically with reference to the center-edge angle, the anterior center-edge angle, the acetabular index and the femoral head extrusion index. Shenton's line was intact in 9 hips before the operation and it was intact in 40 hips at the latest follow-up. The cross-over sign was present in 15 hips before the operation and it was present in one hip after the operation. The results of this study demonstrated that triple pelvic osteotomy provides improved radiographic results and good symptomatic relief in acetabular dysplasia.
[Mh] Termos MeSH primário: Luxação Congênita de Quadril/cirurgia
Ílio/cirurgia
Ísquio/cirurgia
Osteotomia/métodos
Osso Púbico/cirurgia
[Mh] Termos MeSH secundário: Acetábulo/diagnóstico por imagem
Adolescente
Adulto
Criança
Feminino
Seguimentos
Luxação Congênita de Quadril/diagnóstico por imagem
Seres Humanos
Ílio/diagnóstico por imagem
Ísquio/diagnóstico por imagem
Masculino
Meia-Idade
Complicações Pós-Operatórias/epidemiologia
Osso Púbico/diagnóstico por imagem
Radiografia
Remissão Espontânea
Estudos Retrospectivos
Neuropatia Ciática/epidemiologia
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171129
[St] Status:MEDLINE


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[PMID]:28912156
[Au] Autor:Lindborg JA; Mack M; Zigmond RE
[Ad] Endereço:Department of Neurosciences, Case Western Reserve University, Cleveland, Ohio 44106-4975, and.
[Ti] Título:Neutrophils Are Critical for Myelin Removal in a Peripheral Nerve Injury Model of Wallerian Degeneration.
[So] Source:J Neurosci;37(43):10258-10277, 2017 Oct 25.
[Is] ISSN:1529-2401
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Wallerian degeneration (WD) is considered an essential preparatory stage to the process of axonal regeneration. In the peripheral nervous system, infiltrating monocyte-derived macrophages, which use the chemokine receptor CCR2 to gain entry to injured tissues from the bloodstream, are purportedly necessary for efficient WD. However, our laboratory has previously reported that myelin clearance in the injured sciatic nerve proceeds unhindered in the mouse model. Here, we extensively characterize WD in male mice and identify a compensatory mechanism of WD that is facilitated primarily by neutrophils. In response to the loss of CCR2, injured sciatic nerves demonstrate prolonged expression of neutrophil chemokines, a concomitant extended increase in the accumulation of neutrophils in the nerve, and elevated phagocytosis by neutrophils. Neutrophil depletion substantially inhibits myelin clearance after nerve injury in both male WT and mice, highlighting a novel role for these cells in peripheral nerve degeneration that spans genotypes. The accepted view in the basic and clinical neurosciences is that the clearance of axonal and myelin debris after a nerve injury is directed primarily by inflammatory CCR2 macrophages. However, we demonstrate that this clearance is nearly identical in WT and mice, and that neutrophils replace CCR2 macrophages as the primary phagocytic cell. We find that neutrophils play a major role in myelin clearance not only in mice but also in WT mice, highlighting their necessity during nerve degeneration in the peripheral nervous system. These degeneration studies may propel improvements in nerve regeneration and draw critical parallels to mechanisms of nerve degeneration and regeneration in the CNS and in the context of peripheral neuropathies.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Bainha de Mielina/metabolismo
Neutrófilos/metabolismo
Neuropatia Ciática/metabolismo
Degeneração Walleriana/metabolismo
[Mh] Termos MeSH secundário: Animais
Feminino
Masculino
Camundongos
Camundongos da Linhagem 129
Camundongos Endogâmicos C57BL
Camundongos Knockout
Bainha de Mielina/patologia
Compressão Nervosa/métodos
Neutrófilos/patologia
Traumatismos dos Nervos Periféricos/metabolismo
Traumatismos dos Nervos Periféricos/patologia
Fagocitose/fisiologia
Distribuição Aleatória
Neuropatia Ciática/patologia
Degeneração Walleriana/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171115
[Lr] Data última revisão:
171115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1523/JNEUROSCI.2085-17.2017


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[PMID]:28777065
[Au] Autor:Renno WM; Benov L; Khan KM
[Ad] Endereço:Departments of 1 Anatomy and.
[Ti] Título:Possible role of antioxidative capacity of (-)-epigallocatechin-3-gallate treatment in morphological and neurobehavioral recovery after sciatic nerve crush injury.
[So] Source:J Neurosurg Spine;27(5):593-613, 2017 Nov.
[Is] ISSN:1547-5646
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE This study examined the capacity of the major polyphenolic green tea extract (-)-epigallocatechin-3-gallate (EGCG) to suppress oxidative stress and stimulate the recovery and prompt the regeneration of sciatic nerve after crush injury. METHODS Adult male Wistar rats were randomly assigned to one of 4 groups: 1) Naïve, 2) Sham (sham injury, surgical control group), 3) Crush (sciatic nerve crush injury treated with saline), and 4) Crush+EGCG (sciatic nerve crush injury treated with intraperitoneally administered EGCG, 50 mg/kg). All animals were tested for motor and sensory neurobehavioral parameters throughout the study. Sciatic nerve and spinal cord tissues were harvested and processed for morphometric and stereological analysis. For the biochemical assays, the time points were Day 1, Day 7, Day 14, and Day 28 after nerve injury. RESULTS After sciatic nerve crush injury, the EGCG-treated animals (Crush+EGCG group) showed significantly better recovery of foot position and toe spread and 50% greater improvement in motor recovery than the saline-treated animals (Crush group). The Crush+EGCG group displayed an early hopping response at the beginning of the 3rd week postinjury. Animals in the Crush+EGCG group also showed a significant reduction in mechanical allodynia and hyperalgesia latencies and significant improvement in recovery from nociception deficits in both heat withdrawal and tail flick withdrawal latencies compared with the Crush group. In both the Crush+EGCG and Crush groups, quantitative evaluation revealed significant morphological evidence of neuroregeneration according to the following parameters: mean cross-sectional area of axons, myelin thickness in the sciatic nerve (from Week 4 to Week 8), increase of myelin basic protein concentration and gene expression in both the injured sciatic nerve and spinal cord, and fiber diameter to axon diameter ratio and myelin thickness to axon diameter ratio at Week 2 after sciatic nerve injury. However, the axon area remained much smaller in both the Crush+EGCG and Crush groups compared with the Sham and Naïve groups. The number of axons per unit area was significantly decreased in the Crush+EGCG and Crush groups compared with controls. Sciatic nerve injury produced generalized oxidative stress manifested as a significant increase of isoprostanes in the urine and decrease of the total antioxidant capacity (TAC) of the blood from Day 7 until Day 14. EGCG-treated rats showed significantly less increase of isoprostanes than saline-treated animals and also showed full recovery of TAC levels by Day 14 after nerve injury. In spinal cord tissue analysis, EGCG-treated animals showed induced glutathione reductase and suppressed induction of heme oxygenase 1 gene expression compared with nontreated animals. CONCLUSIONS EGCG treatment suppressed the crush-induced production of isoprostanes and stimulated the recovery of the TAC and was associated with remarkable alleviation of motor and sensory impairment and significant histomorphological evidence of neuronal regeneration following sciatic nerve crush injury in rats. The findings of this study suggest that EGCG can be used as an adjunctive therapeutic remedy for nerve injury. However, further investigations are needed to establish the antioxidative mechanism involved in the regenerative process after nerve injury. Only upregulation of glutathione reductase supports the idea that EGCG is acting indirectly via induction of enzymes or transcription factors.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Catequina/análogos & derivados
Lesões por Esmagamento/tratamento farmacológico
Traumatismos dos Nervos Periféricos/tratamento farmacológico
Nervo Isquiático/efeitos dos fármacos
Nervo Isquiático/lesões
[Mh] Termos MeSH secundário: Animais
Axônios/efeitos dos fármacos
Axônios/patologia
Catequina/farmacologia
Lesões por Esmagamento/patologia
Lesões por Esmagamento/fisiopatologia
Modelos Animais de Doenças
Hiperalgesia/tratamento farmacológico
Hiperalgesia/patologia
Hiperalgesia/fisiopatologia
Masculino
Atividade Motora/efeitos dos fármacos
Atividade Motora/fisiologia
Regeneração Nervosa/efeitos dos fármacos
Regeneração Nervosa/fisiologia
Fármacos Neuroprotetores/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Estresse Oxidativo/fisiologia
Traumatismos dos Nervos Periféricos/patologia
Traumatismos dos Nervos Periféricos/fisiopatologia
RNA Mensageiro/metabolismo
Distribuição Aleatória
Ratos Wistar
Recuperação de Função Fisiológica/efeitos dos fármacos
Nervo Isquiático/patologia
Nervo Isquiático/fisiopatologia
Neuropatia Ciática/tratamento farmacológico
Neuropatia Ciática/patologia
Neuropatia Ciática/fisiopatologia
Medula Espinal/efeitos dos fármacos
Medula Espinal/patologia
Medula Espinal/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Neuroprotective Agents); 0 (RNA, Messenger); 8R1V1STN48 (Catechin); BQM438CTEL (epigallocatechin gallate)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170805
[St] Status:MEDLINE
[do] DOI:10.3171/2016.10.SPINE16218


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Texto completo SciELO Brasil
[PMID]:28767917
[Au] Autor:Peretti AL; Antunes JS; Lovison K; Kunz RI; Castor LRG; Brancalhão RMC; Bertolini GRF; Ribeiro LFC
[Ad] Endereço:Universidade Estadual do Oeste do Paraná, Cascavel, PR, Brazil.
[Ti] Título:Action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after nerve injury.
[So] Source:Einstein (Sao Paulo);15(2):186-191, 2017 Apr-Jun.
[Is] ISSN:2317-6385
[Cp] País de publicação:Brazil
[La] Idioma:eng; por
[Ab] Resumo:Objective: To evaluate the action of vanillin (Vanilla planifolia) on the morphology of tibialis anterior and soleus muscles after peripheral nerve injury. Methods: Wistar rats were divided into four groups, with seven animals each: Control Group, Vanillin Group, Injury Group, and Injury + Vanillin Group. The Injury Group and the Injury + Vanillin Group animals were submitted to nerve injury by compression of the sciatic nerve; the Vanillin Group and Injury + Vanillin Group, were treated daily with oral doses of vanillin (150mg/kg) from the 3rd to the 21st day after induction of nerve injury. At the end of the experiment, the tibialis anterior and soleus muscles were dissected and processed for light microscopy and submitted to morphological analysis. Results: The nerve compression promoted morphological changes, typical of denervation, and the treatment with vanillin was responsible for different responses in the studied muscles. For the tibialis anterior, there was an increase in the number of satellite cells, central nuclei and fiber atrophy, as well as fascicular disorganization. In the soleus, only increased vascularization was observed, with no exacerbation of the morphological alterations in the fibers. Conclusion: The treatment with vanillin promoted increase in intramuscular vascularization for the muscles studied, with pro-inflammatory potential for tibialis anterior, but not for soleus muscle. Objetivo: Avaliar a ação da vanilina (Vanilla planifolia) sobre a morfologia dos músculos tibial anterior e sóleo após lesão nervosa periférica. Métodos: Ratos Wistar foram divididos em quatro grupos, com sete animais cada, sendo Grupo Controle, Grupo Vanilina, Grupo Lesão e Grupo Lesão + Vanilina. Os animais dos Grupos Lesão e Grupo Lesão + Vanilina foram submetidos à lesão nervosa por meio da compressão do nervo isquiático, e os Grupos Vanilina e Grupo Lesão + Vanilina foram tratados diariamente com doses orais de vanilina (150mg/kg) do 3o ao 21o dia após a indução da lesão nervosa. Ao término do experimento, os músculos tibial anterior e sóleo foram dissecados e seguiram o processamento de rotina em microscopia de luz, para posterior análise morfológica. Resultados: A compressão nervosa promoveu alterações morfológicas características de denervação, sendo que o tratamento com vanilina foi responsável por respostas distintas nos músculos estudados. Para o tibial anterior, houve aumento do número de células satélites, núcleos centrais e atrofia das fibras, bem como desorganização fascicular. Já no sóleo, houve apenas aumento da vascularização, sem exacerbação das alterações morfológicas nas fibras. Conclusão: O tratamento com vanilina promoveu o aumento da vascularização intramuscular para os músculos estudados, com potencial pró-inflamatório para o tibial anterior, o que não ocorreu no músculo sóleo.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Benzaldeídos/farmacologia
Tecido Conjuntivo/efeitos dos fármacos
Músculo Esquelético/efeitos dos fármacos
Neuropatia Ciática/patologia
[Mh] Termos MeSH secundário: Animais
Tecido Conjuntivo/patologia
Seres Humanos
Masculino
Modelos Animais
Fibras Musculares Esqueléticas/efeitos dos fármacos
Músculo Esquelético/patologia
Distribuição Aleatória
Ratos Wistar
Neuropatia Ciática/reabilitação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Benzaldehydes); CHI530446X (vanillin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE


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[PMID]:28682956
[Au] Autor:Ji ZH; Liu ZJ; Liu ZT; Zhao W; Williams BA; Zhang HF; Li L; Xu SY
[Ad] Endereço:From the *Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China; and †Department of Anesthesiology, University of Pittsburgh, Pittsburgh, Pennsylvania.
[Ti] Título:Diphenyleneiodonium Mitigates Bupivacaine-Induced Sciatic Nerve Damage in a Diabetic Neuropathy Rat Model by Attenuating Oxidative Stress.
[So] Source:Anesth Analg;125(2):653-661, 2017 Aug.
[Is] ISSN:1526-7598
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Increased oxidative stress has been linked to local anesthetic-induced nerve injury in a diabetic neuropathy (DN) rat model. The current study explores the effects of diphenyleneiodonium (DPI) chloride, an NADPH oxidase (NOX) inhibitor, on bupivacaine-induced sciatic nerve injury in DN rats. METHODS: A rat DN model was established through high-fat diet feeding and streptozotocin injection. The model was confirmed via testing (i) blood glucose, (ii) hindpaw allodynia responses to von Frey (VF) monofilaments, (iii) paw withdrawal thermal latency (PWTL), and (iv) nerve conduction velocity (NCV). Bupivacaine (Bup, 0.2 mL, 5 mg/mL) was used to block the right sciatic nerve. DPI (1 mg/kg) was injected subcutaneously 24 hours and 30 minutes before the sciatic block. At 24 hours after the block, NCV, various reactive oxygen species, and Caspase-3 were evaluated to determine the extent of sciatic nerve injury. RESULTS: The DN rat model was successfully established. Compared with the DN control group, the postblock values of VF responses (DN-Con, 16.5 ± 1.3 g; DN + Bup, 19.1 ± 1.5 g, P < .001) and PWTL significantly increased (DN-Con, 13.3 ± 1.1 seconds; DN + Bup, 14.6 ± 1.1 seconds, P = .028); the NCV of sciatic nerve was significantly reduced (DN-Con, 38.8 ± 2.4 m/s, DN + Bup, 30.5 ± 2.0 m/s, P = .003), and sciatic nerve injury (as indicated by axonal area) was more severe in the bupivacaine-treated DN group (DN-Con, 11.6 ± 0.3 µm, DN + Bup, 7.5 ± 0.3 µm, P < .001). In addition, DPI treatment significantly improved nerve function (VF responses, 17.3 ± 1.3 g; PWTL, 13.4 ± 1.1 seconds; NCV, 35.6 ± 3.1 m/s) and mitigated loss of axonal area (9.6 ± 0.3 µm). Compared to the DN + Bup group (without DPI), the levels of lipid peroxides and hydroperoxides, as well as the protein expression of NOX2, NOX4, and Caspase-3, were significantly reduced in the DN + Bup + DPI group (P < .05). CONCLUSIONS: Subcutaneous injection of DPI appears to protect against the functional and neurohistological damage of bupivacaine-blocked sciatic nerves in a high-fat diet/streptozotocin-induced DN model.
[Mh] Termos MeSH primário: Anestésicos Locais/administração & dosagem
Anestésicos Locais/efeitos adversos
Bupivacaína/efeitos adversos
Neuropatias Diabéticas/fisiopatologia
Estresse Oxidativo/efeitos dos fármacos
Nervo Isquiático/lesões
[Mh] Termos MeSH secundário: Animais
Eletrofisiologia
Inibidores Enzimáticos/uso terapêutico
Hiperalgesia/fisiopatologia
Peróxidos Lipídicos/química
Masculino
Bloqueio Nervoso
Oniocompostos/uso terapêutico
Traumatismos dos Nervos Periféricos/induzido quimicamente
Ratos
Ratos Sprague-Dawley
Neuropatia Ciática/fisiopatologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anesthetics, Local); 0 (Enzyme Inhibitors); 0 (Lipid Peroxides); 0 (Onium Compounds); 6HJ411TU98 (diphenyleneiodonium); Y8335394RO (Bupivacaine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1213/ANE.0000000000002186


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[PMID]:28667930
[Au] Autor:Fekrazad R; Mortezai O; Pedram M; Kalhori KA; Joharchi K; Mansoori K; Ebrahimi R; Mashhadiabbas F
[Ad] Endereço:Department of Periodontology, Dental Faculty - Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran; International Network for Photo Medicine and Photo Dynamic Therapy (INPMPDT), Universal Scientific Education and Research Network (USERN), Tehran, Iran.
[Ti] Título:Transected sciatic nerve repair by diode laser protein soldering.
[So] Source:J Photochem Photobiol B;173:441-447, 2017 Aug.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVE: Despite advances in microsurgical techniques, repair of peripheral nerve injuries (PNI) is still a major challenge in regenerative medicine. The standard treatment for PNI includes suturing and anasthomosis of the transected nerve. The objective of this study was to compare neurorraphy (nerve repair) using standard suturingto diode laser protein soldering on the functional recovery of transected sciatic nerves. STUDY DESIGN/MATERIALS AND METHODS: Thirty adult male Fischer-344 Wistar rats were randomly assigned to 3 groups: 1. The control group, no repair, 2. the standard of care suture group, and 3. The laser/protein solder group. For all three groups, the sciatic nerve was transected and the repair was done immediately. For the suture repair group, 10.0 prolene suture was used and for the laser/protein solder group a diode laser (500mW output power) in combination with bovine serum albumen and indocyanine green dye was used. Behavioral assessment by sciatic functional index was done on all rats biweekly. At 12weeks post-surgery, EMG recordings were done on all the rats and the rats were euthanized for histological evaluation of the sciatic nerves. The one-way ANOVA test was used for statistical analysis. RESULTS: The average time required to perform the surgery was significantly shorter for the laser-assisted nerve repair group compared to the suture group. The EMG evaluation revealed no difference between the two groups. Based on the sciatic function index the laser group was significantly better than the suture group after 12weeks (p<0.05). Histopathologic evaluation indicated that the epineurium recovery was better in the laser group (p<0.05). There was no difference in the inflammation between the suture and laser groups. CONCLUSION: Based on this evidence, laser/protein nerve soldering is a more efficient and efficacious method for repair of nerve injury compared to neurorraphy using standard suturing methods.
[Mh] Termos MeSH primário: Lasers Semicondutores/uso terapêutico
Neuropatia Ciática/cirurgia
Soroalbumina Bovina/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Bovinos
Eletromiografia
Verde de Indocianina/química
Masculino
Ratos
Ratos Wistar
Regeneração/efeitos da radiação
Nervo Isquiático/diagnóstico por imagem
Nervo Isquiático/patologia
Nervo Isquiático/fisiologia
Neuropatia Ciática/diagnóstico por imagem
Neuropatia Ciática/patologia
Soroalbumina Bovina/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
27432CM55Q (Serum Albumin, Bovine); IX6J1063HV (Indocyanine Green)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170702
[St] Status:MEDLINE


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[PMID]:28604466
[Au] Autor:Kim E; Cucchiaro G
[Ad] Endereço:From the Department of Anesthesiology Critical Care Medicine, Children's Hospital Los Angeles, Los Angeles, California.
[Ti] Título:Unique Considerations in Spinal Cord Stimulator Placement in Pediatrics: A Case Report.
[So] Source:A A Case Rep;9(4):112-115, 2017 Aug 15.
[Is] ISSN:2325-7237
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Spinal cord stimulation can be a valuable treatment option in the management of neuropathic pain in select pediatric patients. We present a unique case of a 16-year-old girl with Klippel-Trenaunay-Weber syndrome and scoliosis who required the placement of a spinal cord stimulator (SCS) for severe sciatic nerve neuropathic pain after a right above-knee amputation. Several attempts at lead placement were required before successful pain coverage was achieved because of late recognition of significant vertebral body rotation. This case highlights important considerations in pediatric SCS placement including a careful review of the spinal cord anatomy before the placement of an SCS.
[Mh] Termos MeSH primário: Neuroma/complicações
Manejo da Dor/métodos
Neoplasias do Sistema Nervoso Periférico/complicações
Neuropatia Ciática/complicações
Estimulação da Medula Espinal/métodos
[Mh] Termos MeSH secundário: Adolescente
Feminino
Seres Humanos
Síndrome de Klippel-Trenaunay-Weber/complicações
Neuroma/terapia
Neoplasias do Sistema Nervoso Periférico/terapia
Neuropatia Ciática/terapia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE
[do] DOI:10.1213/XAA.0000000000000541



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