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[PMID]:29506509
[Au] Autor:Zhang Z; Zhou M; Liu K; Zhu B; Liu H; Sun X; Xu X
[Ad] Endereço:Shanghai Key Laboratory of Ocular Fundus Diseases, Shanghai, China.
[Ti] Título:Development of a new valid and reliable microsurgical skill assessment scale for ophthalmology residents.
[So] Source:BMC Ophthalmol;18(1):68, 2018 Mar 05.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: More and more concerns have been arisen about the ability of new medical graduates to meet the demands of today's practice environment. In this study, we wanted to develop a valid, reliable and standardized assessment tool for evaluating the basic microsurgical skills of residents in a microsurgery laboratory, to get them well prepared before entering the surgical realm of ophthalmology. METHODS: Twenty-three experts who have teaching experience reviewed the assessment scale. Constructive comments were incorporated to ensure face and content validity. Twenty-one attendings from different specialties then graded eight corneal rupture suturing videos with the scale to investigate interrater reliability. Fourteen of them graded the same videos 3 months later to investigate intrarater reliability (repeatability). RESULTS: A total of 280 assessment scales were completed. All the ICC values of interrater reliability were greater than 0.8 with 75% data greater than 0.9 (range 0.860-0.976). All the ICC values of intrarater reliability (repeatability) were also greater than 0.8 with 63% data greater than 0.9 (range 0.833-0.954). CONCLUSIONS: The assessment scale we developed is valid and reliable. This tool could be useful to ensure that junior residents achieve a certain level of microsurgical technique in a laboratory environment before training in the operation room. Hopefully, this tool will provide a structured template for other residency programs to assess their residents for basic microsurgical skills.
[Mh] Termos MeSH primário: Competência Clínica/normas
Avaliação Educacional/métodos
Internato e Residência
Microcirurgia/educação
Procedimentos Cirúrgicos Oftalmológicos/educação
Oftalmologia/educação
Técnicas de Sutura/educação
[Mh] Termos MeSH secundário: Lesões da Córnea/cirurgia
Seres Humanos
Reprodutibilidade dos Testes
Inquéritos e Questionários
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180311
[Lr] Data última revisão:
180311
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180307
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-018-0736-z


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[PMID]:29422758
[Au] Autor:Liu TS; Meskin S
[Ad] Endereço:Department of Ophthalmology and Visual Science, Yale School of Medicine, New Haven, CT, USA.
[Ti] Título:Localized Trichiasis Causing Focal Full-Thickness Corneal Edema, Endothelial Cell Loss, and Corneal Scarring Requiring Penetrating Keratoplasty.
[So] Source:Middle East Afr J Ophthalmol;24(4):216-218, 2017 Oct-Dec.
[Is] ISSN:0975-1599
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:The purpose of the study was to report a case of focal trichiasis causing full-thickness corneal edema, scarring, and endothelial cell loss requiring penetrating keratoplasty (PK). A 66-year-old male was referred for trichiasis of the right upper eyelid corresponding to an area of full-thickness corneal edema. No keratic precipitates or guttata was noted. Specular microscopy showed diffuse endothelial cell loss. He was treated with topical steroids and acyclovir with epilation of lashes. Anterior chamber paracentesis was negative for varicella-zoster virus, cytomegalovirus, and herpes simplex virus. The patient developed diffuse stromal scarring with a decrease in vision and ultimately underwent PK with preceding eyelid repair. To the best of our knowledge, we present the first reported case of chronic trichiasis causing full-thickness corneal edema, scarring, and endothelial cell loss requiring PK.
[Mh] Termos MeSH primário: Edema da Córnea/etiologia
Perda de Células Endoteliais da Córnea/etiologia
Lesões da Córnea/etiologia
Ceratoplastia Penetrante
Triquíase/complicações
[Mh] Termos MeSH secundário: Idoso
Edema da Córnea/cirurgia
Perda de Células Endoteliais da Córnea/cirurgia
Lesões da Córnea/cirurgia
Seres Humanos
Masculino
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180223
[Lr] Data última revisão:
180223
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180210
[St] Status:MEDLINE
[do] DOI:10.4103/meajo.MEAJO_304_16


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[PMID]:29217021
[Au] Autor:Ross M; Deschênes J
[Ad] Endereço:Department of Ophthalmology, McGill University Health Center, McGill University, Montreal, Que.. Electronic address: michael.ross4@mail.mcgill.ca.
[Ti] Título:Practice patterns in the interdisciplinary management of corneal abrasions.
[So] Source:Can J Ophthalmol;52(6):548-551, 2017 Dec.
[Is] ISSN:1715-3360
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To characterize the treatment and follow-up patterns of corneal abrasions at an academic health centre. METHODS: This is a retrospective review of 90 cases of corneal abrasions over a 2-year period at a tertiary care academic hospital, of which 75 were seen by the ophthalmology department. All consultations primarily for corneal abrasion, as determined by the emergency department physician, were included in the study. Information on treatment regimen, corneal findings by emergency and ophthalmology physicians, time between follow-ups, and final outcomes was collected. RESULTS: Seventy-five patients were seen by ophthalmology a median of 1 day after the emergency room visit. Twenty-five of these patients did not arrive for their subsequent follow-up appointment. Twenty-two of the abrasions were healed by the time of the ophthalmology examination, 51 patients had unhealed corneal abrasions, and 2 had corneal ulcers. Management was changed in 29 of the patients by ophthalmology. The most common management changes were hypertonic saline ointment for prophylaxis or treatment of recurrent erosion syndrome, followed by bandage contact lenses for comfort. CONCLUSIONS: Corneal abrasions are a common condition, and practice patterns for follow-up vary widely. Although the vast majority of patients do very well and likely would heal on their own without ophthalmology referral, it seems reasonable that patients with corneal abrasions are assessed once by an ophthalmologist immediately or possibly up to 1-2 days after the initial emergency visit, depending on the individual patient circumstances.
[Mh] Termos MeSH primário: Lesões da Córnea/terapia
Epitélio Anterior/lesões
Oftalmologistas/estatística & dados numéricos
Equipe de Assistência ao Paciente
Padrões de Prática Médica/estatística & dados numéricos
[Mh] Termos MeSH secundário: Centros Médicos Acadêmicos
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Bandagens
Lentes de Contato
Serviço Hospitalar de Emergência
Feminino
Seres Humanos
Masculino
Meia-Idade
Pomadas
Equipe de Assistência ao Paciente/estatística & dados numéricos
Estudos Retrospectivos
Solução Salina Hipertônica/administração & dosagem
Centros de Atenção Terciária
Cicatrização
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Nm] Nome de substância:
0 (Ointments); 0 (Saline Solution, Hypertonic)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE


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[PMID]:28973369
[Au] Autor:Pal-Ghosh S; Tadvalkar G; Stepp MA
[Ad] Endereço:Department of Anatomy and Regenerative Biology, The George Washington University Medical School, Washington, D.C., United States.
[Ti] Título:Alterations in Corneal Sensory Nerves During Homeostasis, Aging, and After Injury in Mice Lacking the Heparan Sulfate Proteoglycan Syndecan-1.
[So] Source:Invest Ophthalmol Vis Sci;58(12):4959-4975, 2017 Oct 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To determine the impact of the loss of syndecan 1 (SDC1) on intraepithelial corneal nerves (ICNs) during homeostasis, aging, and in response to 1.5-mm trephine and debridement injury. Methods: Whole-mount corneas are used to quantify ICN density and thickness over time after birth and in response to injury in SDC1-null and wild-type (WT) mice. High-resolution three-dimensional imaging is used to visualize intraepithelial nerve terminals (INTs), axon fragments, and lysosomes in corneal epithelial cells using antibodies against growth associated protein 43 (GAP43), ßIII tubulin, and LAMP1. Quantitative PCR was performed to quantify expression of SDC1, SDC2, SDC3, and SDC4 in corneal epithelial mRNA. Phagocytosis was assessed by quantifying internalization of fluorescently labeled 1-µm latex beads. Results: Intraepithelial corneal nerves innervate the corneas of SDC1-null mice more slowly. At 8 weeks, ICN density is less but thickness is greater. Apically projecting intraepithelial nerve terminals and lysosome-associated membrane glycoprotein 1 (LAMP1) are also reduced in unwounded SDC1-null corneas. Quantitative PCR and immunofluorescence studies show that SDC3 expression and localization are increased in SDC1-null ICNs. Wild-type and SDC1-null corneas lose ICN density and thickness as they age. Recovery of axon density and thickness after trephine but not debridement wounds is slower in SDC1-null corneas compared with WT. Experiments assessing phagocytosis show reduced bead internalization by SDC1-null epithelial cells. Conclusions: Syndecan-1 deficiency alters ICN morphology and homeostasis during aging, reduces epithelial phagocytosis, and impairs reinnervation after trephine but not debridement injury. These data provide insight into the mechanisms used by sensory nerves to reinnervate after injury.
[Mh] Termos MeSH primário: Envelhecimento/fisiologia
Córnea/inervação
Lesões da Córnea/patologia
Homeostase/fisiologia
Fibras Nervosas/patologia
Sindecana-1/fisiologia
[Mh] Termos MeSH secundário: Animais
Axônios
Lesões da Córnea/metabolismo
Modelos Animais de Doenças
Epitélio Anterior/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
Sindecana-1/deficiência
Sindecanas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Syndecan-1); 0 (Syndecans)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171004
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-21531


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[PMID]:28850636
[Au] Autor:Bhogal M; Lwin CN; Seah XY; Peh G; Mehta JS
[Ad] Endereço:Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore.
[Ti] Título:Allogeneic Descemet's Membrane Transplantation Enhances Corneal Endothelial Monolayer Formation and Restores Functional Integrity Following Descemet's Stripping.
[So] Source:Invest Ophthalmol Vis Sci;58(10):4249-4260, 2017 Aug 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To characterize the differences in corneal endothelial wound healing in the presence or absence of Descemet's membrane (DM), in vivo. Methods: New-Zealand white rabbits were subjected to 7-mm endothelial wound either by scraping (n = 8; DM intact), peeling (n = 6; DM removed), or a combinatory scrape/peel wound (n = 6). In a second experiment, rabbits underwent peel wound with immediate transplantation of pure decellularized human DM (n = 4). In vivo endothelial migration was assessed via trypan blue staining. Recovery of corneal clarity and thickness was performed by using slit-lamp biomicroscopy and optical coherence tomography. Cell proliferation, phenotype, and morphology were assessed by using immunofluorescence and scanning electron microscopy. Results: In vivo wound closure was faster in the presence of DM; 25.4% ± 1.4%/d versus 5.5% ± 0.6%/d (P < 0.0001). At day 8, complete wound closure was seen in all of the scrape samples but none of the peel group, with wound closure preceding clinical recovery by approximately 6 days in the scrape group. Endothelial cells in the scraped areas reformed functional monolayers capable of restoring corneal thickness and transparency whilst those in the peeled area underwent mesenchymal-like transformation resulting in scar formation. Transplanting decellularized DM in animals receiving a peel wound resulted in clarity and thickness comparable to the scrape group. Endothelial proliferation (Ki67 +ve cells) was higher in scraped versus peeled areas: 54.7% ± 3.5% vs. 8.8% ± 0.7%, (P < 0.01). Conclusions: The presence of DM promoted endothelial wound healing, proliferation, and maintenance of a normal phenotype. DM transplantation recovered the abnormal peel phenotype back to that observed after endothelial scraping.
[Mh] Termos MeSH primário: Lesões da Córnea/cirurgia
Lâmina Limitante Posterior/cirurgia
Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior/métodos
Epitélio Posterior/cirurgia
[Mh] Termos MeSH secundário: Animais
Proliferação Celular/fisiologia
Lesões da Córnea/patologia
Lesões da Córnea/fisiopatologia
Modelos Animais de Doenças
Epitélio Posterior/citologia
Coelhos
Recuperação de Função Fisiológica/fisiologia
Transplante Homólogo
Cicatrização/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170830
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-22106


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[PMID]:28844046
[Au] Autor:Jiang Z; Liu G; Meng F; Wang W; Hao P; Xiang Y; Wang Y; Han R; Li F; Wang L; Li X
[Ad] Endereço:Clinical College of Ophthalmology, Tianjin Medical University, Tianjin, China.
[Ti] Título:Paracrine effects of mesenchymal stem cells on the activation of keratocytes.
[So] Source:Br J Ophthalmol;101(11):1583-1590, 2017 Nov.
[Is] ISSN:1468-2079
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:AIMS: The purpose of this study is to investigate the impact of mesenchymal stem cell (MSC)-derived soluble factors on the function of keratocytes, with a particular focus on the processes involved in wound healing, including keratocyte activation, migration and proliferation as well as extracellular matrix (ECM) synthesis. METHODS: Primary cultured rabbit keratocytes were treated with MSC-conditioned medium (MSC-CM). The paracrine factors released by bone marrow MSCs were examined by ELISA. Time-lapse microscope was used to examine wound closure in vitro. Mouse model of corneal injury was made by epithelial scraping after ethanol injury. RESULTS: MSC-CM significantly increased the wound closure rate of corneal stromal cells in vitro. This enhancement of wound closure by MSC-CM was due to the promotion of cell migration. MSC-CM enhanced keratocyte survival following ethanol injury via inhibiting apoptosis. The expression of ECM component genes in keratocytes was upregulated by MSC-CM. In addition, MSC-CM promoted corneal epithelial wound healing following chemical injury. A number of wound healing mediators were detected in MSC-CM, including vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), hepatocyte growth factor (HGF), transforming growth factor beta 1 (TGFß1), interleukin 8 (IL8), interleukin 6 (IL6) and monocyte chemoattractant protein 1 (MCP1). CONCLUSION: MSC secretes certain factors that accelerate corneal re-epithelialisation. The paracrine effects of MSC on corneal wound healing including improvements in cell viability, migration and ECM formation.
[Mh] Termos MeSH primário: Terapia Baseada em Transplante de Células e Tecidos/métodos
Lesões da Córnea/metabolismo
Ceratócitos da Córnea/patologia
Células Mesenquimais Estromais/metabolismo
Cicatrização
[Mh] Termos MeSH secundário: Animais
Sobrevivência Celular
Células Cultivadas
Lesões da Córnea/patologia
Lesões da Córnea/terapia
Ceratócitos da Córnea/metabolismo
Modelos Animais de Doenças
Ensaio de Imunoadsorção Enzimática
Matriz Extracelular/metabolismo
Células Mesenquimais Estromais/citologia
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170828
[St] Status:MEDLINE
[do] DOI:10.1136/bjophthalmol-2016-310012


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[PMID]:28803936
[Au] Autor:Swamynathan S; Loughner CL; Swamynathan SK
[Ad] Endereço:Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, USA.
[Ti] Título:Inhibition of HUVEC tube formation via suppression of NFκB suggests an anti-angiogenic role for SLURP1 in the transparent cornea.
[So] Source:Exp Eye Res;164:118-128, 2017 Nov.
[Is] ISSN:1096-0007
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Previously, we have reported that the Secreted Ly6/uPAR related protein-1 (SLURP1) serves an important immunomodulatory function in the ocular surface. Here, we examine the involvement of SLURP1 in regulating corneal angiogenic privilege. Slurp1 expression detected by QPCR, immunoblots and immunofluorescent stain, was significantly decreased in mouse corneas subjected to alkali burn-induced corneal neovascularization (CNV). Addition of exogenous SLURP1 (6XHis-tagged, E. coli expressed and partially purified using Ni-ion columns) significantly suppressed the tumor necrosis factor-α (TNF-α)-stimulated human umbilical cord vascular endothelial cell (HUVEC) tube formation. SLURP1 suppressed the HUVEC tube length, tube area and number of branch points, without affecting their viability and/or proliferation. Exogenous SLURP1 in HUVEC also suppressed the TNF-α-induced (i) interleukin-8 (IL-8) and TNF-α production, (ii) adhesion to different components of the extracellular matrix, (iii) migration, and (iv) nuclear localization of NFκB. Together, these results demonstrate that SLURP1 suppresses HUVEC tube formation by blocking nuclear translocation of NFκB, and suggest a potential role for SLURP1 in promoting corneal angiogenic privilege.
[Mh] Termos MeSH primário: Inibidores da Angiogênese/farmacologia
Antígenos Ly/farmacologia
Neovascularização da Córnea/metabolismo
Células Endoteliais da Veia Umbilical Humana/efeitos dos fármacos
NF-kappa B/metabolismo
Ativador de Plasminogênio Tipo Uroquinase/farmacologia
[Mh] Termos MeSH secundário: Animais
Antígenos Ly/fisiologia
Queimaduras Químicas/metabolismo
Adesão Celular/efeitos dos fármacos
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Lesões da Córnea/metabolismo
Modelos Animais de Doenças
Queimaduras Oculares/metabolismo
Seres Humanos
Interleucina-8/metabolismo
Camundongos
Fator de Necrose Tumoral alfa/metabolismo
Ativador de Plasminogênio Tipo Uroquinase/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiogenesis Inhibitors); 0 (Antigens, Ly); 0 (Interleukin-8); 0 (NF-kappa B); 0 (SLURP-1 protein, mouse); 0 (Tumor Necrosis Factor-alpha); EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170815
[St] Status:MEDLINE


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[PMID]:28700779
[Au] Autor:Gallego-Muñoz P; Ibares-Frías L; Lorenzo E; Marcos S; Peréz-Merino P; Bekesi N; Kochevar IE; Martínez-García MC
[Ad] Endereço:Departamento de Biología Celular, Histología y Farmacología, GIR de Técnicas Ópticas para el Diagnóstico, Universidad de Valladolid, Valladolid, Spain.
[Ti] Título:Corneal Wound Repair After Rose Bengal and Green Light Crosslinking: Clinical and Histologic Study.
[So] Source:Invest Ophthalmol Vis Sci;58(9):3471-3480, 2017 Jul 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To evaluate corneal wound healing after treatment with a new collagen crosslinking protocol using rose bengal dye and green light (RGX). Methods: One cornea of 20 New Zealand rabbits was de-epithelialized (DE) in an 8-mm diameter circle and, in another group (n = 25), the DE corneas were then stained with 0.1% rose bengal for 2 minutes and exposed to green light (532 nm) for 7 minutes (RGX). The contralateral eyes without treatment acted as controls. The animals were clinically followed including fluorescein staining and pachymetry. Healing events were analyzed after euthanasia at 2, 30, and 60 days. Cell death (TUNEL assay), cell proliferation (5-bromo-2'-deoxyuridine incorporation), and cell differentiation to myofibroblasts (α-SMA labeling) were carried out. In addition, loss of keratocytes and subsequent repopulation of the corneal stroma were quantified on hematoxylin-eosin-stained sections. Results: Wound closure was slower after RGX (4.4 days) then after DE (3.3 days). Cell death was restricted to the anterior central stroma, and the cellular decrease did not differ significantly between RGX and DE corneas. Cell proliferation in the epithelium and stroma appeared at 2 days. In both DE and RGX corneas, recovery of the epithelium was complete at day 30, although cell repopulation of the stroma was not complete at 60 days. Conclusions: The healing response in corneas after RGX is very similar to that observed after DE alone, suggesting that, along with its short treatment time and limited effect on keratocytes, RGX displays good potential for clinical cornea stiffening.
[Mh] Termos MeSH primário: Colágeno/farmacologia
Córnea/patologia
Lesões da Córnea/tratamento farmacológico
Reagentes para Ligações Cruzadas/farmacologia
Luz
Rosa Bengala/farmacologia
Cicatrização/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Córnea/efeitos dos fármacos
Modelos Animais de Doenças
Feminino
Corantes Fluorescentes/farmacologia
Coelhos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Fluorescent Dyes); 1ZPG1ELY14 (Rose Bengal); 9007-34-5 (Collagen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170713
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-21365


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[PMID]:28657877
[Au] Autor:Gupta PC; Ram J
[Ad] Endereço:Postgraduate Institute of Medical Education and Research, Chandigarh, India drjagatram@gmail.com.
[Ti] Título:Eye Injury from a Firecracker.
[So] Source:N Engl J Med;376(26):2579, 2017 Jun 29.
[Is] ISSN:1533-4406
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Traumatismos por Explosões/patologia
Corpos Estranhos no Olho/patologia
Traumatismos Oculares/patologia
[Mh] Termos MeSH secundário: Adulto
Traumatismos por Explosões/diagnóstico por imagem
Lesões da Córnea/diagnóstico por imagem
Corpos Estranhos no Olho/diagnóstico por imagem
Traumatismos Oculares/diagnóstico por imagem
Seres Humanos
Masculino
Ruptura/diagnóstico por imagem
Tomografia Computadorizada por Raios X
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170718
[Lr] Data última revisão:
170718
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:170629
[St] Status:MEDLINE
[do] DOI:10.1056/NEJMicm1616104


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[PMID]:28644870
[Au] Autor:Kim JW; Lim CW; Kim B
[Ad] Endereço:Biosafety Research Institute and Laboratory of Pathology (BK21 Plus Program), College of Veterinary Medicine, Chonbuk National University, Iksan, Republic of Korea.
[Ti] Título:Effects of nicotine on corneal wound healing following acute alkali burn.
[So] Source:PLoS One;12(6):e0179982, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Epidemiological studies have indicated that smoking is a pivotal risk factor for the progression of several chronic diseases. Nicotine, the addictive component of cigarettes, has powerful pathophysiological properties in the body. Although the effects of cigarette smoking on corneal re-epithelialization have been studied, the effects of nicotine on corneal wound healing-related neovascularization and fibrosis have not been fully demonstrated. The aim of this study was to evaluate the effects of chronic administration of nicotine on corneal wound healing following acute insult induced by an alkali burn. BALB/C female mice randomly received either vehicle (2% saccharin) or nicotine (100 or 200 µg/ml in 2% saccharin) in drinking water ad libitum. After 1 week, animals were re-randomized and the experimental group was subjected to a corneal alkali burn, and then nicotine was administered until day 14 after the alkali burn. A corneal alkali burn model was generated by placing a piece of 2 mm-diameter filter paper soaked in 1N NaOH on the right eye. Histopathological analysis and the expression level of the pro-angiogenic genes vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP9) revealed that chronic nicotine administration enhanced alkali burn-induced corneal neovascularization. Furthermore, the mRNA expression of the pro-fibrogenic factors α-smooth muscle actin (αSMA), transforming growth factor-ß (TGF-ß), and collagen α1 (Col1) was enhanced in the high-concentration nicotine-treated group compared with the vehicle group after corneal injury. Immunohistochemical analysis also showed that the αSMA-positive area was increased in chronic nicotine-treated mice after corneal alkali burn. An in vitro assay found that expression of the α3, α7, and ß1 nicotinic acetylcholine receptor (nAChR) subunits was significantly increased by chemical injury in human corneal fibroblast cells. Moreover, alkali-induced fibrogenic gene expression and proliferation of fibroblast cells were further increased by treatment with nicotine and cotinine. The proliferation of such cells induced by treatment of nicotine and cotinine was reduced by inhibition of the PI3K and PKC pathways using specific inhibitors. In conclusion, chronic administration of nicotine accelerated the angiogenic and fibrogenic healing processes in alkali-burned corneal tissue.
[Mh] Termos MeSH primário: Queimaduras Químicas/tratamento farmacológico
Lesões da Córnea/tratamento farmacológico
Queimaduras Oculares/tratamento farmacológico
Nicotina/farmacologia
Substâncias Protetoras/farmacologia
Cicatrização/efeitos dos fármacos
[Mh] Termos MeSH secundário: Actinas/metabolismo
Animais
Queimaduras Químicas/metabolismo
Queimaduras Químicas/patologia
Linhagem Celular
Colágeno Tipo I/metabolismo
Córnea/irrigação sanguínea
Córnea/efeitos dos fármacos
Córnea/metabolismo
Córnea/patologia
Lesões da Córnea/induzido quimicamente
Lesões da Córnea/metabolismo
Lesões da Córnea/patologia
Cotinina
Modelos Animais de Doenças
Queimaduras Oculares/induzido quimicamente
Queimaduras Oculares/metabolismo
Queimaduras Oculares/patologia
Feminino
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Fibroblastos/patologia
Seres Humanos
Camundongos Endogâmicos BALB C
Distribuição Aleatória
Receptores Nicotínicos/metabolismo
Hidróxido de Sódio
Fator de Crescimento Transformador beta/metabolismo
Fator A de Crescimento do Endotélio Vascular/metabolismo
Cicatrização/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acta2 protein, mouse); 0 (Actins); 0 (Collagen Type I); 0 (Protective Agents); 0 (Receptors, Nicotinic); 0 (Transforming Growth Factor beta); 0 (Vascular Endothelial Growth Factor A); 0 (vascular endothelial growth factor A, mouse); 55X04QC32I (Sodium Hydroxide); 6M3C89ZY6R (Nicotine); K5161X06LL (Cotinine)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170624
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179982



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