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[PMID]:29390522
[Au] Autor:Ko J; Kim SK; Yong DE; Kim TI; Kim EK
[Ad] Endereço:Department of Ophthalmology, Corneal Dystrophy Research Institute, Institute of Vision Research, Yonsei University College of Medicine.
[Ti] Título:Delayed onset Mycobacterium intracellulare keratitis after laser in situ keratomileusis: A case report and literature review.
[So] Source:Medicine (Baltimore);96(51):e9356, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Infectious keratitis is a relatively uncommon but potentially sight-threatening complication of laser in situ keratomileusis (LASIK). Mycobacterial keratitis is usually regarded as late onset keratitis among post-LASIK keratitis. There has been no documented case of Mycobacterium intracellulare post-LASIK keratitis of a long-latent period. PATIENT CONCERNS: A 36-year-old man was referred to our out-patient clinic, for persistent corneal epithelial defect with intrastromal infiltration. He had undergone uneventful bilateral LASIK procedure 4 years before. He complained decreased vision, accompanied by ocular pain, photophobia, and redness in his left eye for 7 months. DIAGNOSIS: Lamellar keratectomy was taken using femtosecond laser. Bacterial culture with sequenced bacterial 16s ribosomal DNA confirmed the organism to be M intracellulare. INTERVENTIONS: After 3 months of administration of topical clarithromycin, amikacin, and moxifloxacin, the corneal epithelial defect was resolved and the infiltration was much improved. However, newly developed diffuse haziness with surrounding granular infiltration in the central cornea was noted. Drug toxicity was suspected and topical moxifloxacin was discontinued, resulting in resolution of the diffuse haze with infiltration. OUTCOME: The patient was followed up regularly without medication thereafter and recurrence was not found for 7 years. LESSONS: This case presents the first case of M intracellulare keratitis after LASIK. LASIK surgeons should aware that post-LASIK keratitis can develop long after the operation and careful suspicion of infectious disease with meticulous diagnostic test is needed.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Infecções Oculares Bacterianas/diagnóstico
Ceratite/microbiologia
Ceratomileuse Assistida por Excimer Laser In Situ/efeitos adversos
Complexo Mycobacterium avium/isolamento & purificação
[Mh] Termos MeSH secundário: Administração Tópica
Adulto
Amicacina/uso terapêutico
Claritromicina/uso terapêutico
Quimioterapia Combinada
Infecções Oculares Bacterianas/tratamento farmacológico
Infecções Oculares Bacterianas/etiologia
Fluoroquinolonas/uso terapêutico
Seguimentos
Seres Humanos
Ceratite/tratamento farmacológico
Ceratite/etiologia
Ceratomileuse Assistida por Excimer Laser In Situ/métodos
Masculino
Índice de Gravidade de Doença
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Fluoroquinolones); 84319SGC3C (Amikacin); H1250JIK0A (Clarithromycin); U188XYD42P (moxifloxacin)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180203
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009356


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[PMID]:28460048
[Au] Autor:Kolar SS; Baidouri H; McDermott AM
[Ad] Endereço:The Ocular Surface Institute, University of Houston, College of Optometry, Houston, Texas, United States.
[Ti] Título:Role of Pattern Recognition Receptors in the Modulation of Antimicrobial Peptide Expression in the Corneal Epithelial Innate Response to F. solani.
[So] Source:Invest Ophthalmol Vis Sci;58(5):2463-2472, 2017 05 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: Fusarium solani (F. solani) keratitis is a potentially sight-threatening fungal infection of the cornea. Antimicrobial peptides (AMPs), such as human ß-defensins (hBDs) and cathelicidins, essential components of the immune system, likely have a protective role against F. solani keratitis. We examined the role of pattern recognition receptors (PRRs), Dectin-1, and TLR2 in F. solani-induced modulation of AMP expression in vitro. Methods: Human corneal epithelial cells (HCECs) were exposed to heat-inactivated F. solani or pathogen-associated molecular patterns (PAMPs) of F. solani (Zymosan or Zymosan Depleted) for 6, 12, or 24 hours following which AMP mRNA and protein levels were determined. Involvement of TLR2 and Dectin-1 was confirmed by using siRNA knock-down (TLR2 and Dectin-1) or chemical inhibitor BAY 61-3606 (Dectin-1). The functional significance of AMP upregulation was tested using culture supernatant from F. solani or PAMP-treated HCECs against F. solani in the presence of hBD2 or LL37 neutralizing antibody. Results: We confirm that HCECs express Dectin-1 and TLR2. HCECs demonstrated upregulation of AMPs hBD2 and cathelicidin LL37 following exposure to heat-inactivated F. solani or PAMPs. TLR2 and Dectin-1 knockdown and BAY 61-3606 treatment decreased AMP mRNA upregulation confirming PRR involvement. The culture supernatant from F. solani or PAMP-treated HCECs showed substantial killing of F. solani and hBD2 or LL37 neutralizing antibody significantly decreased this effect implicating involvement of these AMPs. Conclusions: These findings demonstrate that Dectin-1 and TLR2 have an important role in regulating F. solani-induced AMP expression in corneal epithelial cells.
[Mh] Termos MeSH primário: Peptídeos Catiônicos Antimicrobianos/genética
Epitélio Anterior/metabolismo
Infecções Oculares Fúngicas/metabolismo
Fusariose/metabolismo
Regulação da Expressão Gênica
Ceratite/metabolismo
Receptores de Reconhecimento de Padrão/genética
[Mh] Termos MeSH secundário: Peptídeos Catiônicos Antimicrobianos/biossíntese
Células Cultivadas
Córnea/metabolismo
Córnea/patologia
Epitélio Anterior/microbiologia
Epitélio Anterior/patologia
Infecções Oculares Fúngicas/imunologia
Citometria de Fluxo
Fusariose/diagnóstico
Fusariose/microbiologia
Seres Humanos
Immunoblotting
Ceratite/diagnóstico
Ceratite/microbiologia
RNA/genética
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Receptores de Reconhecimento de Padrão/metabolismo
Transdução de Sinais
Regulação para Cima
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antimicrobial Cationic Peptides); 0 (RNA, Messenger); 0 (Receptors, Pattern Recognition); 63231-63-0 (RNA)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180124
[Lr] Data última revisão:
180124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-20658


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[PMID]:29310395
[Au] Autor:Kuo CY; Chang YF; Chou YB; Hsu CC; Lin PY; Liu CJ
[Ad] Endereço:Department of Ophthalmology, Taipei Veterans General Hospital.
[Ti] Título:Delayed onset of pressure-induced interlamellar stromal keratitis in a patient with recurrent uveitis: A case report.
[So] Source:Medicine (Baltimore);96(48):e8958, 2017 Dec.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: Corticosteroid treatment for uveitis can lead to delayed-onset pressure-induced interlamellar stromal keratitis (PISK), even years after laser in situ keratomileusis (LASIK).A 35-year-old man presented to our clinic after experiencing blurred vision in his left eye for 1 month. For the past month, he had been prescribed topical steroid and anti-glaucomatous medication. He had undergone LASIK for both eyes 5 years earlier, and had suffered uveitis attacks in his left eye over the last 2 years.Slit-lamp examination revealed stromal haziness with interface fluid accumulation in the left eye. The left eye showed an intraocular pressure (IOP) of 35 mm Hg and visual acuity of 6/20. Anterior segment ocular coherence tomography (OCT) confirmed the diagnosis of PISK. Steroid treatment was tapered, and latanoprost treatment was started. One month later, the patient's symptoms resolved, with IOP reduced to 10 mm Hg and visual acuity increased to 6/6 in the left eye. Latanoprost treatment was discontinued to avoid potential uveitis reactivation, and the patient's visual field defect progressed and IOP rebounded. Due to evident glaucomatous damage, trabeculectomy was suggested but was refused. CONCLUSION: Patients with PISK plus uveitis should be treated with a tailored regimen involving corticosteroid and antiglaucomatous medication or surgical intervention based on the individual condition. Early recognition and appropriate treatment may aid in preventing severe visual sequela in such patients.
[Mh] Termos MeSH primário: Ceratite/complicações
Ceratite/tratamento farmacológico
Uveíte/complicações
[Mh] Termos MeSH secundário: Adulto
Progressão da Doença
Seres Humanos
Pressão Intraocular
Ceratite/diagnóstico por imagem
Ceratite/fisiopatologia
Masculino
Transtornos da Visão/etiologia
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008958


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[PMID]:29242901
[Au] Autor:Clemens LE; Jaynes J; Lim E; Kolar SS; Reins RY; Baidouri H; Hanlon S; McDermott AM; Woodburn KW
[Ad] Endereço:Riptide Bioscience, Inc., Vallejo, California, United States.
[Ti] Título:Designed Host Defense Peptides for the Treatment of Bacterial Keratitis.
[So] Source:Invest Ophthalmol Vis Sci;58(14):6273-6281, 2017 Dec 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To limit corneal damage and potential loss of vision, bacterial keratitis must be treated aggressively. Innovation in antimicrobials is required due to the need for empirical treatment and the rapid emergence of bacterial resistance. Designed host defense peptides (dHDPs) are synthetic analogues of naturally occurring HDPs, which provide defense against invading pathogens. This study investigates the use of novel dHDPs for the treatment of bacterial keratitis. Methods: The minimum inhibitory concentrations (MICs) were determined for dHDPs on both Gram-positive and -negative bacteria. The minimum biofilm eradication concentrations (MBEC) and in vitro time-kill assays were determined. The most active dHDP, RP444, was evaluated for propensity to induce drug resistance and therapeutic benefit in a murine Pseudomonas aeruginosa keratitis model. Results: Designed HDPs were bactericidal with MICs ranging from 2 to >64 µg/mL and MBEC ranging from 6 to 750 µg/mL. In time-kill assays, dHDPs were able to rapidly reduce bacterial counts upon contact with as little as 2 µg/mL. RP444 did not induce resistance after repeated exposure of P. aeruginosa to subinhibitory concentrations. RP444 demonstrated significant efficacy in a murine model of bacterial keratitis as evidenced by a significant dose-dependent decrease in ocular clinical scores, a significantly reduced bacterial load, and substantially decreased inflammatory cell infiltrates. Conclusions: Innovative dHDPs demonstrated potent antimicrobial activity, possess a limited potential for development of resistance, and reduced the severity of murine P. aeruginosa keratitis. These studies demonstrate that a novel dHDP may have potential to treat patients with sight-threatening bacterial keratitis.
[Mh] Termos MeSH primário: Biofilmes/efeitos dos fármacos
Córnea/microbiologia
Infecções Oculares Bacterianas/tratamento farmacológico
Ceratite/tratamento farmacológico
Compostos de Organotecnécio/administração & dosagem
Peptídeos Cíclicos/administração & dosagem
Infecções por Pseudomonas/tratamento farmacológico
Pseudomonas aeruginosa/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Meios de Cultura Livres de Soro
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Infecções Oculares Bacterianas/microbiologia
Ceratite/microbiologia
Camundongos Endogâmicos C57BL
Testes de Sensibilidade Microbiana
Infecções por Pseudomonas/microbiologia
Pseudomonas aeruginosa/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (99mTc(HYNICtide)(tricine)(TPPTS)); 0 (Culture Media, Serum-Free); 0 (Organotechnetium Compounds); 0 (Peptides, Cyclic)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171216
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-22243


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[PMID]:28452995
[Au] Autor:Tan SZ; Walkden A; Au L; Fullwood C; Hamilton A; Qamruddin A; Armstrong M; Brahma AK; Carley F
[Ad] Endereço:Centre for Ophthalmology and Vision Sciences, Faculty of Medical and Human Sciences, University of Manchester, Manchester, UK.
[Ti] Título:Twelve-year analysis of microbial keratitis trends at a UK tertiary hospital.
[So] Source:Eye (Lond);31(8):1229-1236, 2017 Aug.
[Is] ISSN:1476-5454
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PurposeTo investigate the frequencies, trends, and in vitro drug susceptibilities of the causative pathogens in microbial keratitis in Manchester Royal Eye Hospital.Patients and methodsCorneal scrape results recorded by the microbiology service between 2004 and 2015 were extracted from an established database. A total of 4229 corneal scrape specimens were identified from an established database. First-line antibiotic treatment in our centre during the study period was ofloxacin and second line was cefuroxime and gentamicin.ResultsMean age was 45.9±21.0. A total of 1379 samples (32.6%) were culture positive. One hundred forty-eight (10.7%) specimens cultured multiple organisms. Of the 1539 organisms identified, 63.3% were Gram-positive bacteria, 27.3% Gram-negative bacteria, 7.1% fungi, and 2.3% Acanthamoebae. A decreasing trend in Gram-positive isolates was found together with a stable trend in Gram negatives and an increasing trend in Acanthamoeba and fungi. There appeared to be a significant increasing trend of Moraxella infection (P=0.001). In all, 83.1 and 90.8% of Gram-positive and -negative isolates tested were susceptible to ofloxacin, respectively. Cefuroxime covered 86.6% of Gram-positive and 61.4% of Gram-negative isolates, whereas gentamicin covered 88.8 and 96.5% of Gram-positive and -negative isolates, respectively.ConclusionWe found a change in the type of Gram-negative organisms isolated over time, with the Moraxella species on the rise. Reassuringly, no significant increase in resistance was observed in vitro for any of the commonly used antibiotics. Ofloxacin remains a good first-line antibiotic treatment but duo-therapy does have broader coverage and should be considered in non-responsive cases.
[Mh] Termos MeSH primário: Antibacterianos/uso terapêutico
Infecções Oculares Bacterianas/microbiologia
Ceratite/microbiologia
[Mh] Termos MeSH secundário: Acanthamoeba/isolamento & purificação
Adulto
Idoso
Antibacterianos/farmacologia
Úlcera da Córnea/microbiologia
Farmacorresistência Bacteriana/efeitos dos fármacos
Infecções Oculares Bacterianas/tratamento farmacológico
Feminino
Fungos/isolamento & purificação
Bactérias Gram-Negativas/efeitos dos fármacos
Bactérias Gram-Negativas/isolamento & purificação
Bactérias Gram-Positivas/efeitos dos fármacos
Bactérias Gram-Positivas/isolamento & purificação
Seres Humanos
Ceratite/tratamento farmacológico
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Estudos Retrospectivos
Centros de Atenção Terciária/estatística & dados numéricos
Reino Unido
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171221
[Lr] Data última revisão:
171221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1038/eye.2017.55


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[PMID]:29216872
[Au] Autor:Li M; Yang D; Chen Y; Li M; Han T; Zhou X; Ni K
[Ad] Endereço:Key Lab of Myopia, Ministry of Health, Department of Ophthalmology, EYE & ENT Hospital of Fudan University, Shanghai, China.
[Ti] Título:Late-onset diffuse lamellar keratitis 4 years after femtosecond laser-assisted small incision lenticule extraction: a case report.
[So] Source:BMC Ophthalmol;17(1):244, 2017 Dec 08.
[Is] ISSN:1471-2415
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: To report a first case of late-onset diffuse lamellar keratitis (DLK) occurring 4 years after femtosecond laser-assisted small incision lenticule extraction (SMILE). CASE PRESENTATION: A 41-year-old man who underwent SMILE 4 years prior developed DLK in the right eye 1 day after he was struck in the eye by a finger while playing with his son. Slim-lamp microscopy and anterior segment optical coherence tomography (AS-OCT) were used to evaluate the cornea of the right eye. Slit-lamp examination of the right eye revealed epithelial exfoliation and stage 3 DLK with diffuse, dot-like, granular haze in the interface between the cap and stromal bed. After intensive treatment with topical corticosteroids, the DLK resolved and corneal transparency was achieved. CONCLUSIONS: This case indicates that DLK can occur several years after SMILE. Ocular trauma may be a risk factor for the development of DLK. The prognosis is usually favorable with early diagnosis and treatment with topical corticosteroids.
[Mh] Termos MeSH primário: Substância Própria/cirurgia
Traumatismos Oculares/complicações
Ceratite/etiologia
Ceratomileuse Assistida por Excimer Laser In Situ
Complicações Pós-Operatórias
[Mh] Termos MeSH secundário: Adulto
Seres Humanos
Masculino
Fatores de Tempo
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171209
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-017-0641-x


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[PMID]:29019876
[Au] Autor:Motozawa N; Nakamura T; Takagi S; Fujihara M; Hirami Y; Ishida K; Sotozono C; Kurimoto Y
[Ad] Endereço:aDepartment of Ophthalmology, Kobe City Medical Center General Hospital bDepartment of Ophthalmology, Institute of Biomedical Research and Innovation Hospital, Kobe cDepartment of Frontier Medical Science and Technology for Ophthalmology dDepartment of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
[Ti] Título:Unique circumferential peripheral keratitis in relapsing polychondritis: A case report.
[So] Source:Medicine (Baltimore);96(41):e7951, 2017 Oct.
[Is] ISSN:1536-5964
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:RATIONALE: Relapsing polychondritis (RP) is a rare collagen disease characterized by inflammation and destruction of cartilage throughout the body. The paper details the clinical course of a case of RP with unique circumferential peripheral keratitis. PATIENT CONCERNS: A 54-year-old Japanese woman was referred to the hospital presenting with auricular and ocular pain. DIAGNOSES: Based on the auricle biopsy results and the three presenting symptoms (bilateral auricular chondritis, inflammatory arthritis and ocular inflammation), her condition was diagnosed as RP. INTERVENTIONS: The three presenting symptoms gradually improved with prednisolone (PSL), methylprednisolone and cyclophosphamide combination therapy, followed by PSL, methotrexate and infliximab combination therapy. However, one month after the initial visit, despite ongoing treatment, a unique circumferential peripheral keratitis suddenly occurred, in which the corneal infiltration gradually clumped together and shrank at the peripheral area. The eye and ear pain showed exacerbations and remissions on reducing the dosage of steroid drugs. The general condition was improved on altering systemic therapy to PSL, methotrexate and tocilizumab. OUTCOMES: Keratitis gradually disappeared within 10 months of the initial visit. LESSONS: This is the first report of a case of RP causing unique circumferential peripheral keratitis. This keratitis occurred despite use of focal and systemic steroids and showed improvement with general recovery. This may indicate that stabilization of general condition is important for recovery from keratitis in RP.
[Mh] Termos MeSH primário: Anticorpos Monoclonais Humanizados/administração & dosagem
Artrite
Ciclofosfamida/administração & dosagem
Infliximab/administração & dosagem
Ceratite
Policondrite Recidivante
Prednisolona/administração & dosagem
[Mh] Termos MeSH secundário: Antirreumáticos/administração & dosagem
Artrite/etiologia
Artrite/patologia
Técnicas de Diagnóstico Oftalmológico
Monitoramento de Medicamentos/métodos
Quimioterapia Combinada/métodos
Cartilagem da Orelha/patologia
Feminino
Seres Humanos
Ceratite/tratamento farmacológico
Ceratite/etiologia
Ceratite/patologia
Ceratite/fisiopatologia
Metotrexato/administração & dosagem
Meia-Idade
Policondrite Recidivante/complicações
Policondrite Recidivante/diagnóstico
Policondrite Recidivante/tratamento farmacológico
Policondrite Recidivante/fisiopatologia
Resultado do Tratamento
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies, Monoclonal, Humanized); 0 (Antirheumatic Agents); 8N3DW7272P (Cyclophosphamide); 9PHQ9Y1OLM (Prednisolone); B72HH48FLU (Infliximab); I031V2H011 (tocilizumab); YL5FZ2Y5U1 (Methotrexate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171122
[Lr] Data última revisão:
171122
[Sb] Subgrupo de revista:AIM; IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000007951


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[PMID]:28961278
[Au] Autor:McClellan SA; Jerome A; Suvas S; Hazlett LD
[Ad] Endereço:Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI, United States of America.
[Ti] Título:NLRC4 regulates caspase-1 and IL-1beta production in a CD11blowLy6Glow population of cells required for resistance to Pseudomonas aeruginosa keratitis.
[So] Source:PLoS One;12(9):e0185718, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Psbetaeudomonas (P.) aeruginosa infection of the cornea in BALB/c mice does not result in perforation and the mice have been classified as resistant. However, regulation of this response via inflammasome activation remained untested. Therefore, BALB/c mice were infected with P. aeruginosa ATCC strain 19660 and NLRP3 and NLRC4 protein tested by ELISA. Since NLRC4 vs NLRP3 protein levels were significantly higher in the corneas of BALB/c at 1 and 5 days postinfection we used silencing to knockdown NLRC4. Silencing NLRC4 vs scrambled siRNA treatment exacerbated disease in BALB/c mice, reduced myeloperoxidase levels and elevated bacterial plate counts at 5 days postinfection. It also increased pro IL-1beta, but reduced total protein for IL-1beta and IL-18 at 5 days postinfection. Flow cytometry to identify cells affected by silencing, showed reduced caspase-1 levels in a CD11blowLy6Glow population of cells, (but not PMN or macrophages) from the infected cornea of siNLRC4 treated mice that produced less mature IL-1beta. These data provide evidence that the NLRC4 inflammasome contributes to resistance through regulation of caspase-1, IL-1beta and IL-18 in a CD11blowLy6Glow population of cells.
[Mh] Termos MeSH primário: Proteínas Reguladoras de Apoptose/fisiologia
Antígeno CD11b/imunologia
Proteínas de Ligação ao Cálcio/fisiologia
Caspase 1/biossíntese
Interleucina-1beta/biossíntese
Ceratite/imunologia
Infecções por Pseudomonas/imunologia
Pseudomonas aeruginosa/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Proteínas Reguladoras de Apoptose/genética
Proteínas de Ligação ao Cálcio/genética
Contagem de Colônia Microbiana
Ensaio de Imunoadsorção Enzimática
Feminino
Citometria de Fluxo
Técnicas de Silenciamento de Genes
Ceratite/microbiologia
Camundongos
Camundongos Endogâmicos BALB C
Infecções por Pseudomonas/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Apoptosis Regulatory Proteins); 0 (CD11b Antigen); 0 (Calcium-Binding Proteins); 0 (Interleukin-1beta); 0 (Ipaf protein, mouse); EC 3.4.22.36 (Caspase 1)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170930
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185718


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[PMID]:28936055
[Au] Autor:Al-Amry M; Mudhaiyan T; Al-Huthail R; Al-Ghadeer H
[Ad] Endereço:Anterior Segment Division, King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia.
[Ti] Título:Infectious Crystalline Keratopathy After Corneal Cross-linking.
[So] Source:Middle East Afr J Ophthalmol;24(2):100-102, 2017 Apr-Jun.
[Is] ISSN:0975-1599
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:To report a rare case of infectious keratitis after collagen cross-linking (CXL) for keratoconus. A 20-year-old male patient underwent CXL for keratoconus in his right eye. Four weeks after the procedure, he reported blurred vision and redness with increasing pain in the treated eye. Ophthalmic examination revealed a corneal epithelial defect with corneal infiltrates that exhibited branching needle-like opacities. The patient was diagnosed with infectious crystalline keratopathy (ICK). Corneal scrapings and culture indicated the presence of . The patient was successfully treated with fortified vancomycin and ceftazidime over several weeks. ICK is a potential post-operative complication of CXL that can lead to corneal scarring with a permanent reduction in visual acuity.
[Mh] Termos MeSH primário: Colágeno/efeitos adversos
Córnea/patologia
Reagentes para Ligações Cruzadas/efeitos adversos
Infecções Oculares Bacterianas/microbiologia
Ceratite/microbiologia
Ceratocone/tratamento farmacológico
Infecções Estreptocócicas/microbiologia
[Mh] Termos MeSH secundário: Colágeno/uso terapêutico
Córnea/efeitos dos fármacos
Reagentes para Ligações Cruzadas/uso terapêutico
Infecções Oculares Bacterianas/induzido quimicamente
Infecções Oculares Bacterianas/diagnóstico
Seres Humanos
Ceratite/induzido quimicamente
Ceratite/diagnóstico
Masculino
Infecções Estreptocócicas/induzido quimicamente
Infecções Estreptocócicas/diagnóstico
Streptococcus salivarius/isolamento & purificação
Acuidade Visual
Adulto Jovem
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 9007-34-5 (Collagen)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE
[do] DOI:10.4103/meajo.MEAJO_346_16


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[PMID]:28837732
[Au] Autor:Ranjith K; Sontam B; Sharma S; Joseph J; Chathoth KN; Sama KC; Murthy SI; Shivaji S
[Ad] Endereço:Jhaveri Microbiology Centre, Brien Holden Eye Research Centre, L. V. Prasad Eye Institute, Kallam Anji Reddy campus, Hyderabad, India.
[Ti] Título:Candida Species From Eye Infections: Drug Susceptibility, Virulence Factors, and Molecular Characterization.
[So] Source:Invest Ophthalmol Vis Sci;58(10):4201-4209, 2017 Aug 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To determine the type of Candida species in ocular infections and to investigate the relationship of antifungal susceptibility profile to virulence factors. Methods: Fifty isolates of yeast-like fungi from patients with keratitis, endophthalmitis, and orbital cellulitis were identified by Vitek-2 compact system and DNA sequencing of ITS1-5.8S-ITS2 regions of the rRNA gene, followed by phylogenetic analysis for phenotypic and genotypic identification, respectively. Minimum inhibitory concentration of six antifungal drugs was determined by E test/microbroth dilution methods. Phenotypic and genotypic methods were used to determine the virulence factors. Results: Phylogenetic analysis showed the clustering of all isolates into eight distinct groups with a major cluster formed Candida parapsilosis (n = 21), which was the most common species by both Vitek 2 and DNA sequencing. Using χ2 test no significant difference was noted between the techniques except that Vitek 2 did not identify C. viswanathii, C. orthopsilosis, and two non-Candida genera. Of 43 tested Candida isolates high susceptibility to amphotericin B (39/43, 90.6%) and natamycin (43/43, 100%) was noted. While none of the isolates produced coagulase, all produced esterase and catalase. The potential to form biofilm was detected in 23/43 (53.4%) isolates. Distribution of virulence factors by heat map analysis showed difference in metabolic activity of biofilm producers from nonbiofilm producers. Conclusions: Identified by Vitek 2 and DNA sequencing methods C. parapsilosis was the most common species associated with eye infections. Irrespective of the virulence factors elaborated, the Candida isolates were susceptible to commonly used antifungal drugs such as amphotericin B and natamycin.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Candida
Candidíase/microbiologia
Infecções Oculares Fúngicas/microbiologia
Fatores de Virulência/metabolismo
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Anfotericina B/farmacologia
Biofilmes/crescimento & desenvolvimento
Candida/efeitos dos fármacos
Candida/genética
Candida/isolamento & purificação
Candida/metabolismo
Pré-Escolar
Endoftalmite/microbiologia
Feminino
Seres Humanos
Ceratite/microbiologia
Masculino
Testes de Sensibilidade Microbiana
Meia-Idade
Natamicina/farmacologia
Celulite Orbitária/microbiologia
RNA Fúngico/genética
RNA Ribossômico/genética
Análise de Sequência de DNA
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (RNA, Fungal); 0 (RNA, Ribosomal); 0 (Virulence Factors); 7XU7A7DROE (Amphotericin B); 8O0C852CPO (Natamycin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170904
[Lr] Data última revisão:
170904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170825
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-22003



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