Base de dados : MEDLINE
Pesquisa : C12.706.316.795.124 [Categoria DeCS]
Referências encontradas : 172 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 18 ir para página                         

  1 / 172 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:24630042
[Au] Autor:Remnant JG; Lea RG; Allen CE; Huxley JN; Robinson RS; Brower AI
[Ad] Endereço:School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, United Kingdom. Electronic address: john.remnant@nottingham.ac.uk.
[Ti] Título:Novel gonadal characteristics in an aged bovine freemartin.
[So] Source:Anim Reprod Sci;146(1-2):1-4, 2014 Apr.
[Is] ISSN:1873-2232
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The gonads from a five-year-old freemartin Holstein animal were subjected to morphological analysis and to immunohistochemistry using antibodies against developmental and functional markers. We demonstrate, for the first time, the retention of anti-mullerian hormone (AMH) producing intratubular cells (Sertoli cells) in the context of abundant steroidogenic interstitial cells, and structures consistent with clusters of luteal cells. This novel report describes the clinical, gross and histological findings accompanying this newly described gonadal immunophenotype, and its implication in the understanding of freemartin development.
[Mh] Termos MeSH primário: Envelhecimento
Freemartinismo
Ovário/patologia
Testículo/patologia
[Mh] Termos MeSH secundário: Animais
Bovinos
Feminino
Freemartinismo/patologia
Freemartinismo/fisiopatologia
Masculino
Ovário/crescimento & desenvolvimento
Testículo/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1411
[Cu] Atualização por classe:140324
[Lr] Data última revisão:
140324
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140318
[St] Status:MEDLINE


  2 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:22813600
[Au] Autor:Harikae K; Tsunekawa N; Hiramatsu R; Toda S; Kurohmaru M; Kanai Y
[Ad] Endereço:Department of Veterinary Anatomy, The University of Tokyo, Tokyo 113-8657, Japan.
[Ti] Título:Evidence for almost complete sex-reversal in bovine freemartin gonads: formation of seminiferous tubule-like structures and transdifferentiation into typical testicular cell types.
[So] Source:J Reprod Dev;58(6):654-60, 2012.
[Is] ISSN:1348-4400
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:During mammalian sex determination of XY fetuses, SRY induces SOX9 in Sertoli cells, resulting in formation of testes with seminiferous tubules, interstitial Leydig cells and peritubular myoid cells. Meanwhile XX fetuses without SRY develop ovaries. In cattle, most XX heifers born with a male twin, so-called freemartins, develop nonfunctioning ovaries and genitalia with an intersex phenotype. Interestingly, freemartins sometimes develop highly masculinized gonads with seminiferous tubule-like structures despite the absence of SRY. However, in these cases, the degree of masculinization in each gonadal somatic cell type is unclear. Here, we report a rare case of a freemartin Japanese black calf with almost complete XX sexreversal. Gross anatomical analysis of this calf revealed the presence of a pair of small testis-like gonads with rudimentary epididymides, in addition to highly masculinized genitalia including a pampiniform plexus, scrotum and vesicular gland. Histological and immunohistochemical analyses of these masculinized gonads revealed well-defined seminiferous tubule-like structures throughout the whole gonadal parenchyma. In epithelia of these tubules, SOX9-positive supporting cells (i.e., Sertoli cells) were found to be arranged regularly along the bases of tubules, and they were also positive for GDNF, one of the major factors for spermatogenesis. 3ß-HSD-positive cells (i.e., Leydig cells) and SMA-positive peritubular myoid cells were also identified around tubules. Therefore, for the first time, we found the transdifferentiation of ovarian somatic cells into all testicular somatic cell types in the XX freemartin gonads. These data strongly support the idea of a high sexual plasticity in the ovarian somatic cells of mammalian gonads.
[Mh] Termos MeSH primário: Transdiferenciação Celular
Freemartinismo/patologia
Gônadas/patologia
[Mh] Termos MeSH secundário: 3-Hidroxiesteroide Desidrogenases/metabolismo
Animais
Bovinos
Células Epiteliais/metabolismo
Feminino
Fator Neurotrófico Derivado de Linhagem de Célula Glial/metabolismo
Gônadas/metabolismo
Masculino
Fatores de Transcrição SOX9/metabolismo
Fator Esteroidogênico 1/metabolismo
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Glial Cell Line-Derived Neurotrophic Factor); 0 (SOX9 Transcription Factor); 0 (Steroidogenic Factor 1); EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
[Em] Mês de entrada:1305
[Cu] Atualização por classe:121221
[Lr] Data última revisão:
121221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120721
[St] Status:MEDLINE


  3 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:22095296
[Au] Autor:Jeon BG; Rho GJ; Betts DH; Petrik JJ; Favetta LA; King WA
[Ad] Endereço:Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
[Ti] Título:Low levels of X-inactive specific transcript in somatic cell nuclear transfer embryos derived from female bovine freemartin donor cells.
[So] Source:Sex Dev;6(1-3):151-9, 2012.
[Is] ISSN:1661-5433
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The present study compared developmental potential, telomerase activity and transcript levels of X-linked genes (HPRT, MECP2, RPS4X, SLC25A6, XIAP, XIST and ZFX) in bovine somatic cell nuclear transfer (SCNT) embryos reconstructed with cells derived from a freemartin (female with a male co-twin) or from normal female cattle (control). The rates of cleavage, development to blastocyst and hatched blastocyst stage, and the mean numbers of total and inner cell mass cells in the freemartin SCNT embryos were not significantly different from those of control SCNT embryos (p > 0.05). The levels of telomerase activity analyzed by RQ-TRAP in the freemartin SCNT embryos were also similar to those of the normal SCNT embryos. Transcript levels of HPRT, MECP2, RPS4X and XIAP, measured by quantitative real-time RT-PCR, were not significantly different between the control and freemartin SCNT embryos (p > 0.05). However, the transcript levels of SLC25A6, XIST and ZFX were significantly decreased in the freemartin SCNT embryos compared to control SCNT embryos (p < 0.05). Transfer of 71 freemartin SCNT embryos to 22 recipient cows resulted in 4 (18%) pregnancies, which were lost between days 28 and 90 of gestation. Taken together, the present study demonstrates that the transcript levels of several X-linked genes, especially XIST, showed an aberrant pattern in the freemartin SCNT embryos, suggesting aberrant X inactivation in freemartin clones which may affect embryo survival.
[Mh] Termos MeSH primário: Embrião de Mamíferos/metabolismo
Freemartinismo/genética
Genes Ligados ao Cromossomo X/genética
Técnicas de Transferência Nuclear/veterinária
Inativação do Cromossomo X/genética
Cromossomo X/genética
[Mh] Termos MeSH secundário: Animais
Bovinos
Clonagem de Organismos
Transferência Embrionária/veterinária
Desenvolvimento Embrionário
Feminino
Morte Fetal/genética
Morte Fetal/veterinária
Masculino
Gravidez
RNA Longo não Codificante
RNA Mensageiro/análise
RNA não Traduzido/genética
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Telomerase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Long Noncoding); 0 (RNA, Messenger); 0 (RNA, Untranslated); 0 (XIST non-coding RNA); EC 2.7.7.49 (Telomerase)
[Em] Mês de entrada:1206
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111119
[St] Status:MEDLINE
[do] DOI:10.1159/000334050


  4 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:21376423
[Au] Autor:Pons JA; Revilla-Nuin B; Ramírez P; Baroja-Mazo A; Parrilla P
[Ad] Endereço:Servicio de Aparato Digestivo, Unidad de Hepatología, Hospital Universitario Virgen de la Arrixaca, Murcia, España. joseapons@yahoo.es
[Ti] Título:[Development of immune tolerance in liver transplantation].
[Ti] Título:Desarrollo de inmunotolerancia en el trasplante hepático..
[So] Source:Gastroenterol Hepatol;34(3):155-69, 2011 Mar.
[Is] ISSN:0210-5705
[Cp] País de publicação:Spain
[La] Idioma:spa
[Ab] Resumo:The liver is a privileged organ and has a lower incidence of rejection than other organs. However, immunosuppressive regimens are still required to control the alloreactive T-lymphocyte response after transplantation. These treatments may lead to severe complications, such as infectious diseases, cancers, cardiovascular diseases and chronic renal insufficiency. In clinical transplantation there is increasing evidence that some liver transplant recipients who cease taking immunosuppressive (IS) drugs maintain allograft function, suggesting that tolerance is already present. This strategy is feasible in 25-33% of liver transplant recipients. A series of experimental and clinical observations indicates that liver allografts can even provide "tolerogenic" properties for other organ grafts. In the clinical setting, clinical operational tolerance (COT) is defined as the absence of acute and chronic rejection and graft survival with normal function and histology in an IS-free, fully immunocompetent host, usually as an end result of a successful attempt at IS withdrawal. The exact mechanisms involved in achieving transplant tolerance remain unknown, although animal models suggest a possible role for regulatory T cells (Treg). Recent data have demonstrated an increase in the frequency of CD4+ CD25(high) T cells and FoxP3 transcripts during IS withdrawal in operationally tolerant liver transplant recipients. The data obtained from transcriptional profiling of the peripheral blood of IS-free liver transplant recipients suggest that there is a molecular signature of tolerance that could be employed to identify tolerant liver transplant recipients and that innate immune cells are likely to play a major role in the maintenance of COT after liver transplantation.
[Mh] Termos MeSH primário: Tolerância Imunológica
Transplante de Fígado/imunologia
[Mh] Termos MeSH secundário: Animais
Apresentação do Antígeno
Biomarcadores
Bovinos
Células Dendríticas/classificação
Células Dendríticas/imunologia
Freemartinismo/imunologia
Perfilação da Expressão Gênica
Rejeição de Enxerto/genética
Rejeição de Enxerto/imunologia
Rejeição de Enxerto/prevenção & controle
Sobrevivência de Enxerto/genética
Sobrevivência de Enxerto/imunologia
Seres Humanos
Tolerância Imunológica/genética
Tolerância Imunológica/imunologia
Imunidade Inata
Imunossupressores/efeitos adversos
Imunossupressores/uso terapêutico
Interleucinas/sangue
Camundongos
Especificidade de Órgãos
Seleção de Pacientes
Polimorfismo Genético
Ratos
Subpopulações de Linfócitos T/imunologia
Quimeras de Transplante/imunologia
Imunologia de Transplantes
Transplante Homólogo/imunologia
[Pt] Tipo de publicação:ENGLISH ABSTRACT; JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Biomarkers); 0 (Immunosuppressive Agents); 0 (Interleukins)
[Em] Mês de entrada:1107
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110308
[St] Status:MEDLINE
[do] DOI:10.1016/j.gastrohep.2010.11.007


  5 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:20190264
[Au] Autor:Bogdanova N; Siebers U; Kelsch R; Markoff A; Röpke A; Exeler R; Tsokas J; Wieacker P
[Ad] Endereço:Institute of Human Genetics, ZMBE Westfalian Wilhelms-University of Münster and University Clinics Münster, Münster, Germany.
[Ti] Título:Blood chimerism in a girl with Down syndrome and possible freemartin effect leading to aplasia of the Müllerian derivatives.
[So] Source:Hum Reprod;25(5):1339-43, 2010 May.
[Is] ISSN:1460-2350
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cytogenetic and molecular genetic analysis in a case of sex-discordant dizygotic twins revealed blood chimerism in the girl (46,XY in blood and 47,XX, + 21 in fibroblasts) caused by feto-fetal transfusion from her healthy brother. The girl presented with Down syndrome, aplasia of the uterus and the Fallopian tubes and normal female external genitalia. We propose that the lack of Müllerian structures is caused by the effect of the Müllerian inhibiting substance transferred from the male to the female twin in early pregnancy. This disorder of sex development is known as freemartin phenomenon in female cattle from sex-discordant twin pairs.
[Mh] Termos MeSH primário: Quimera/genética
Síndrome de Down/genética
Freemartinismo/genética
Ductos Paramesonéfricos/anormalidades
[Mh] Termos MeSH secundário: Adulto
Animais
Bovinos
Pré-Escolar
Cromossomos Humanos Y
Síndrome de Down/sangue
Síndrome de Down/imunologia
Feminino
Transfusão Feto-Fetal/sangue
Transfusão Feto-Fetal/genética
Haplótipos
Teste de Histocompatibilidade
Seres Humanos
Hibridização in Situ Fluorescente
Recém-Nascido
Cariotipagem
Linfócitos/ultraestrutura
Masculino
Linhagem
Gravidez
Gêmeos Dizigóticos
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1007
[Cu] Atualização por classe:100414
[Lr] Data última revisão:
100414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100302
[St] Status:MEDLINE
[do] DOI:10.1093/humrep/deq048


  6 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:18467837
[Au] Autor:Brace MD; Peters O; Menzies P; King WA; Nino-Soto MI
[Ad] Endereço:Department of Biomedical Science, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
[Ti] Título:Sex chromosome chimerism and the freemartin syndrome in Rideau Arcott sheep.
[So] Source:Cytogenet Genome Res;120(1-2):132-9, 2008.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:In cattle, nearly all heifers born co-twin to a male are freemartins, XX/XY chimeras that exhibit a characteristic masculinized phenotype. However, in sheep, while litters containing males and females are common, freemartins are relatively rare. The primary aim of this study was to determine the frequency and features of XX/XY chimerism in female Rideau Arcott sheep. Also, breeding records were used to investigate the effect of litter size and sex ratios, as well as the genetic basis of the condition. Finally, the migration and transcriptional competence of cells of the opposite sex in the XX/XY female and male chimeras was explored. Genomic DNA (gDNA) from peripheral blood cells of ewes was screened by PCR for the male-specific SRY gene. Of 230 lambs screened, 10 were identified as chimeras. Litter size and sex ratio showed no statistically significant effect on the frequency of chimerism. PCR and FISH analysis confirmed the presence of opposite sex cells in female and male chimeras, and in the case of ewes, their migration to tissues other than blood. Transcriptional activity of SRY and AMH was detected in gonads of ewes, whereas XIST expression was detected in white blood cells of chimeric rams. It was concluded that the frequency of sex chromosome chimerism in Rideau Arcott sheep is estimated at 4.35%, with no significant effect of litter size and sex ratio. Moreover, as it was shown that opposite sex cells can migrate to tissues other than blood and be transcriptionally active in chimeric sheep, we speculate on the role they can play in these animals.
[Mh] Termos MeSH primário: Quimera/genética
Freemartinismo/genética
Transtornos dos Cromossomos Sexuais/veterinária
Doenças dos Ovinos/genética
Ovinos/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Bovinos
Primers do DNA/genética
Transtornos do Desenvolvimento Sexual/genética
Transtornos do Desenvolvimento Sexual/patologia
Transtornos do Desenvolvimento Sexual/veterinária
Feminino
Freemartinismo/patologia
Genes sry
Hibridização in Situ Fluorescente/veterinária
Tamanho da Ninhada de Vivíparos/genética
Masculino
Fenótipo
Reação em Cadeia da Polimerase
Gravidez
Transtornos dos Cromossomos Sexuais/genética
Transtornos dos Cromossomos Sexuais/patologia
Razão de Masculinidade
Doenças dos Ovinos/patologia
Especificidade da Espécie
Síndrome
Cromossomo X
Cromossomo Y
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:0805
[Cu] Atualização por classe:101118
[Lr] Data última revisão:
101118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080510
[St] Status:MEDLINE
[do] DOI:10.1159/000118752


  7 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:18418032
[Au] Autor:Peretti V; Ciotola F; Albarella S; Paciello O; Dario C; Barbieri V; Iannuzzi L
[Ad] Endereço:Department of Animal Science and Food Inspection, Faculty of Veterinary Medicine, University Federico II, Naples (Italy). vincenzo.peretti@unina.it
[Ti] Título:XX/XY chimerism in cattle: clinical and cytogenetic studies.
[So] Source:Sex Dev;2(1):24-30, 2008.
[Is] ISSN:1661-5433
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The freemartin condition represents the most frequent form of intersexuality found in cattle, and occasionally in other species. Freemartinism arises when vascular connections occur between placentae of developing heterosexual twin foetuses, XX/XY chimerism develops, and ultimately there is masculinisation of the female tubular reproductive tract to varying degrees. The aim of this work was to report the clinical and cytogenetic studies performed in 28 cattle co-twins, 24 of which were cytogenetically chimeric (2n = 60, XX/XY), raised in the region of Campania (southern Italy). Clinical findings of the 16 freemartin females examined varied greatly, from a more female phenotype (normal body conformation with the presence of a blind-ending vagina and primordial ovarian and uterus structures) to a nearly male phenotype (body conformations with male traits and presence of primordial prepuce, penis and testicles). The 8 freemartin males, in spite of the presence of XX cells, had a normal body conformation and external genitalia and some of them were fertile. In addition to cytogenetic diagnosis we also verified chromosome fragility by testing for chromosome aberration (CA: aneuploidy, gaps, chromatid breaks, chromosome breaks and fragments) and sister chromatid exchange (SCE). Freemartins showed a higher percentage of aneuploid cells and significant statistical differences in mean values of gaps, chromatid breaks and chromosome breaks when compared with control animals. To our knowledge, this is the first time that chromosome instability has been evaluated by analyses of CA and SCE in freemartin cattle.
[Mh] Termos MeSH primário: Doenças dos Bovinos/genética
Doenças dos Bovinos/patologia
Bovinos/anormalidades
Freemartinismo/genética
Freemartinismo/patologia
[Mh] Termos MeSH secundário: Animais
Bovinos/genética
Quimera/genética
Citogenética
Feminino
Genitália Feminina/patologia
Genitália Masculina/patologia
Masculino
Troca de Cromátide Irmã/genética
Especificidade da Espécie
Cromossomo X
Cromossomo Y
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:0806
[Cu] Atualização por classe:080417
[Lr] Data última revisão:
080417
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080418
[St] Status:MEDLINE
[do] DOI:10.1159/000117716


  8 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
PubMed Central Texto completo
[PMID]:17988380
[Au] Autor:Niku M; Pessa-Morikawa T; Taponen J; Iivanainen A
[Ad] Endereço:Department of Basic Veterinary Sciences, Division of Anatomy, University of Helsinki, Helsinki, Finland. mikael.niku@helsinki.fi
[Ti] Título:Direct observation of hematopoietic progenitor chimerism in fetal freemartin cattle.
[So] Source:BMC Vet Res;3:29, 2007 Nov 07.
[Is] ISSN:1746-6148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cattle twins are well known as blood chimeras. However, chimerism in the actual hematopoietic progenitor compartment has not been directly investigated. Here, we analyzed fetal liver of chimeric freemartin cattle by combining a new anti-bovine CD34 antibody and Y-chromosome specific in situ hybridization. RESULTS: Bull-derived CD34+ cells were detected in the liver of the female sibling (freemartin) at 60 days gestation. The level of bull-derived CD34+ cells was lower in the freemartin than in its male siblings. Bull (Y+) and cow hematopoietic cells often occurred in separate clusters. Around clusters of Y+CD34+ cells, Y+CD34- cells were typically observed. The thymi were also strongly chimeric at 60 days of gestation. CONCLUSION: The fetal freemartin liver contains clusters of bull-derived hematopoietic progenitors, suggesting clonal expansion and differentiation. Even the roots of the hematopoietic system in cattle twins are thus strongly chimeric from the early stages of fetal development. However, the hematopoietic seeding of fetal liver apparently started already before the onset of functional vascular anastomosis.
[Mh] Termos MeSH primário: Bovinos/embriologia
Freemartinismo/embriologia
Células-Tronco Hematopoéticas/patologia
Fígado/embriologia
[Mh] Termos MeSH secundário: Animais
Antígenos CD34/biossíntese
Bovinos/genética
Quimerismo/embriologia
Quimerismo/veterinária
Feminino
Freemartinismo/genética
Freemartinismo/patologia
Células-Tronco Hematopoéticas/ultraestrutura
Hibridização in Situ Fluorescente/veterinária
Fígado/ultraestrutura
Masculino
Timo/embriologia
Cromossomo Y
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antigens, CD34)
[Em] Mês de entrada:0802
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071109
[St] Status:MEDLINE


  9 / 172 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:17917028
[Au] Autor:Yunis EJ; Zuniga J; Romero V; Yunis EJ
[Ad] Endereço:Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, 44 Binney St., Boston, MA 02115, USA. edmond_yunis@dfci.harvard.edu
[Ti] Título:Chimerism and tetragametic chimerism in humans: implications in autoimmunity, allorecognition and tolerance.
[So] Source:Immunol Res;38(1-3):213-36, 2007.
[Is] ISSN:0257-277X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The presence of cells or tissues from two individuals, chimeras, or the presence of cells and tissues that include the gonads, tetragametic chimerism can be detected by the analysis of cytogenetics and analysis of polymorphic genetic markers, using patterns of pedigree inheritance. These methodologies include determination of sex chromosomes, major histocompatibility complex (MHC) polymorphisms and panels of short tandem repeats (STRs) that include mitochondrial DNA markers. Studies routinely involve cases of temporal chimerism in blood transfusion, or following allotransplantation to measure the outcome of the organ, lymphopoietic tissues or bone marrow grafts. Demonstration of persistent chimerism is usually discovered in cases of inter-sexuality due to fusion of fraternal twins or in cases of fusion of embryos with demonstrable allogeneic monoclonality of blood which, excluded maternity or paternity when blood alone is used as the source of DNA. In single pregnancies it is possible to produce two kinds of microchimerism: feto-maternal and materno-fetal, but in cases of fraternal twin pregnancies it is possible to identify three different kinds which are related to cases of vanishing twins that can be identified during pregnancy by imaging procedures; (1) hematopoietic, (2) gonadal, and (3) freemartins when the twins have different sex and the individual born is a female with either gonadal or both gonadal and hematopoietic tissues. Fraternal twin pregnancies can also produce fusion of embryos. Such cases could be of different sex presenting with inter-sexuality or in same sex twins. One of such cases, the best studied, showed evidence of chimerism and tetragametism. In this regard, the case was studied because of disputed maternity of two of her three children. All tissues studied, except for the blood, demonstrated four genetic components but only two in her blood of four possible showed allogeneic monoclonality consistent with the interpretation that her blood originated from one hematopoietic stem cell. Also, microchimerism, due to traffic of cells via materno-fetal or feto-maternal has been prompted by reports of their potential association with the development of autoimmune disorders including systemic lupus erythematosus (SLE) and systemic sclerosis, and in allotransplantation. In addition, their relevance of chimerism in the positive and negative selection of T cells in the thymus has not been addressed. T lymphocytes play a central role in controlling the acquired immune response and furthermore serve as crucial effector cells through antigen specific cytotoxic activity and the production of soluble mediators. Central tolerance is established by the repertoire selection of immature T lymphocytes in the thymus, avoiding the generation of autoreactive T cells. Expression of chimeric antigens in the thymus could modify the generation of specific T cell clones in chimeric subjects and these mechanisms could be important in the induction of central tolerance against foreign antigens important in allo-transplantation. In this review, we discuss the genetics of chimerism and tetragametism and its potential role in thymic selection and the relevance in allotransplantation and autoimmune disorders.
[Mh] Termos MeSH primário: Autoimunidade
Quimerismo
Tolerância Imunológica
[Mh] Termos MeSH secundário: Animais
Bovinos
Quimerismo/embriologia
DNA Mitocondrial/análise
Feminino
Freemartinismo/diagnóstico
Freemartinismo/genética
Marcadores Genéticos/genética
Células Germinativas/citologia
Hematopoese
Seres Humanos
Masculino
Transplante Homólogo/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (DNA, Mitochondrial); 0 (Genetic Markers)
[Em] Mês de entrada:0801
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071006
[St] Status:MEDLINE


  10 / 172 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
[PMID]:17870153
[Au] Autor:Sohn SH; Cho EJ; Son WJ; Lee CY
[Ad] Endereço:Department of Animal Science and Biotechnology, Jinju National University, Jinju, Republic of Korea. shsohn@jinju.ac.kr
[Ti] Título:Diagnosis of bovine freemartinism by fluorescence in situ hybridization on interphase nuclei using a bovine Y chromosome-specific DNA probe.
[So] Source:Theriogenology;68(7):1003-11, 2007 Oct 15.
[Is] ISSN:0093-691X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A heifer co-twin to a bull, in most cases, is a sterile freemartin which needs to be identified and culled from replacement stock. Various methods are available for the diagnosis of freemartinism, but none is ideal in terms of speed, sensitivity, or specificity. The present study was thus conducted to develop and validate a satisfactory fluorescence in situ hybridization procedure on interphase nuclei (I-FISH) for identifying the bovine XX/XY-karyotypic chimerism, the hallmark of freemartinism. A 190-bp DNA FISH probe containing the bovine male-specific BC1.2 DNA sequence was synthesized and labeled with digoxigenin by PCR. The FISH was performed on metaphase spreads and interphase nuclei of blood lymphocytes. Upon FISH, the probe expectedly bound to the nucleus of the male cell or to a region of the p12 locus of the Y chromosome. Twenty-four young heterosexual twins (Holstein-Friesian and Korean Cattle breeds; 10 pairs and 4 singletons) were analyzed in the present study; all but three exhibited the XX/XY-karyotypic chimerism to varying extents in both I-FISH and karyotyping. One heifer was identified to have 100% XX cells by both analyses, whereas two bulls were judged as 100% XY- and XX/XY-chimeric karyotypes by karyotyping and I-FISH, respectively. Nevertheless, the ratios of the XY to XX cells in these animals were very similar between the two analyses. In conclusion, the present I-FISH was a rapid and reliable procedure that can be used for early-life diagnosis of bovine freemartinism.
[Mh] Termos MeSH primário: Núcleo Celular/genética
Sondas de DNA/metabolismo
Freemartinismo/diagnóstico
Hibridização in Situ Fluorescente/veterinária
Interfase/genética
Linfócitos/citologia
Cromossomo Y/genética
[Mh] Termos MeSH secundário: Animais
Bovinos
Feminino
Masculino
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Probes)
[Em] Mês de entrada:0811
[Cu] Atualização por classe:070924
[Lr] Data última revisão:
070924
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070918
[St] Status:MEDLINE



página 1 de 18 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde