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[PMID]:28239797
[Au] Autor:Savilova AM; Farkhat KN; Yushina MN; Rudimova YV; Makiyan ZN; Adamyan LV
[Ad] Endereço:V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia. bushueva@gmail.com.
[Ti] Título:Characteristics of Multipotent Mesenchymal Stromal Cells Isolated from the Endometrium and Endometriosis Lesions of Women with Malformations of the Internal Reproductive Organs.
[So] Source:Bull Exp Biol Med;162(4):539-544, 2017 Feb.
[Is] ISSN:1573-8221
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We isolated and characterized cell cultures from eutopic endometrium and endometriotic lesions of women with malformations of the internal reproductive organs. The cells had fibroblast-like shape and intensively expressed CD90, CD73, CD105, CD44, CD146, and CD117 and were capable of induced adipogenic and osteogenic differentiation in vitro. The obtained cultures exhibited properties of multipotent mesenchymal stromal cells; at the same time, they demonstrated in vitro immunophenotypic differences from cell cultures of eutopic and ectopic endometrium of women without developmental abnormalities, which suggests their functional difference. The cells from eutopic endometrium and from ectopic endometriotic lesions can be used as the model for studying of the etiology and pathogenesis of endometriosis and for testing new drugs for this specific group of patients. Markers CD90 and CD117 were identified as promising molecules for the development of minimally invasive diagnostics of endometriosis based on cell cultures from eutopic endometrium.
[Mh] Termos MeSH primário: Adipócitos/citologia
Endometriose/patologia
Endométrio/anormalidades
Ginatresia/patologia
Células Mesenquimais Estromais/citologia
Osteoblastos/citologia
[Mh] Termos MeSH secundário: Adipócitos/metabolismo
Adolescente
Adulto
Biomarcadores/metabolismo
Diferenciação Celular
Forma Celular
Endometriose/diagnóstico
Endometriose/metabolismo
Endométrio/metabolismo
Feminino
Expressão Gênica
Ginatresia/diagnóstico
Ginatresia/metabolismo
Seres Humanos
Imunofenotipagem
Células Mesenquimais Estromais/metabolismo
Osteoblastos/metabolismo
Proteínas Proto-Oncogênicas c-kit/genética
Proteínas Proto-Oncogênicas c-kit/metabolismo
Antígenos Thy-1/genética
Antígenos Thy-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers); 0 (Thy-1 Antigens); EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE
[do] DOI:10.1007/s10517-017-3656-7


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[PMID]:28170094
[Au] Autor:Gan L; Duan H; Sun FQ; Xu Q; Tang YQ; Wang S
[Ad] Endereço:Department of Minimally Invasive Gynecologic Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, China.
[Ti] Título:Efficacy of freeze-dried amnion graft following hysteroscopic adhesiolysis of severe intrauterine adhesions.
[So] Source:Int J Gynaecol Obstet;137(2):116-122, 2017 May.
[Is] ISSN:1879-3479
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To evaluate the efficacy of freeze-dried amnion graft for prevention of intrauterine adhesion (IUA) reformation after hysteroscopic adhesiolysis. METHODS: A prospective randomized controlled trial was conducted among 88 women with severe IUAs who underwent hysteroscopic adhesiolysis at Beijing Obstetrics and Gynecology Hospital between July 15, 2015, and July 1, 2016. All participants had a balloon inserted into the uterine cavity for 1 week. Sterilized freeze-dried amnion graft covered the balloon portion of the Foley catheter among patients allocated to the amnion group (n=44), whereas patients in the control group (n=44) did not receive the graft. Follow-up hysteroscopy was performed 3 months after surgery. Preoperative and postoperative IUA scores, menstruation scores, and pregnancy rates were assessed. RESULTS: Both groups exhibited reductions in IUA scores and improvements in menstruation scores following treatment (P<0.001 for each measure). Compared with the control group, the amnion group had a lower IUA score (P=0.032) and a higher menstruation score (P<0.001) at follow-up. By contrast, the rates of IUA reformation and pregnancy were not significantly different between the two groups. CONCLUSION: Use of freeze-dried amnion graft was effective in reducing IUA reformation and improving menstruation (according to pictorial blood-loss assessment chart) following hysteroscopic adhesiolysis of severe IUAs. ClinicalTrials.gov: (NCT02496052).
[Mh] Termos MeSH primário: Âmnio/transplante
Ginatresia/cirurgia
Dispositivos Intrauterinos
Aderências Teciduais/cirurgia
[Mh] Termos MeSH secundário: Adulto
Feminino
Liofilização
Ginatresia/prevenção & controle
Seres Humanos
Histeroscopia
Complicações Pós-Operatórias
Estudos Prospectivos
Aderências Teciduais/prevenção & controle
Transplantes
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170918
[Lr] Data última revisão:
170918
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1002/ijgo.12112


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[PMID]:28148903
[Au] Autor:Wang X; Ma N; Sun Q; Huang C; Liu Y; Luo X
[Ad] Endereço:The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China, 510630.
[Ti] Título:Elevated NF-κB signaling in Asherman syndrome patients and animal models.
[So] Source:Oncotarget;8(9):15399-15406, 2017 Feb 28.
[Is] ISSN:1949-2553
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Asherman syndrome (intrauterine adhesion) is often associated with menstrual abnormalities, infertility and recurrent miscarriage in female. Currently the molecular mechanism regulating the pathogenesis of Asherman syndrome is not known. Here we revealed that the inflammatory factor NF-κB expression is significantly elevated in the endometrial samples of Asherman syndrome patients. To further study the molecular mechanisms, we established an Asherman syndrome rat model and confirmed the important role of NF-κB in the pathogenesis of Asherman syndrome. In addition, our rat model provided direct evidence that intrauterine adhesion results in impaired pregnancy, supporting the clinical association between intrauterine adhesion and mis-regulated pregnancy. Our result identified NF-κB as a novel pathogenesis factor of Asherman syndrome and provided new insights for the prevention and treatment of intrauterine adhesions in Asherman syndrome patients.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Endométrio/metabolismo
Ginatresia/genética
NF-kappa B/genética
Transdução de Sinais/genética
[Mh] Termos MeSH secundário: Animais
Western Blotting
Feminino
Expressão Gênica
Ginatresia/metabolismo
Ginatresia/patologia
Seres Humanos
Imuno-Histoquímica
NF-kappa B/metabolismo
Gravidez
Ratos
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (NF-kappa B)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.18632/oncotarget.14853


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[PMID]:27856386
[Au] Autor:Chen L; Zhang H; Wang Q; Xie F; Gao S; Song Y; Dong J; Feng H; Xie K; Sui L
[Ad] Endereço:Medical Center of Diagnosis and Treatment for Cervical Diseases, Obstetrics and Gynecology Hospital, Fudan University, and Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Shanghai, China.
[Ti] Título:Reproductive Outcomes in Patients With Intrauterine Adhesions Following Hysteroscopic Adhesiolysis: Experience From the Largest Women's Hospital in China.
[So] Source:J Minim Invasive Gynecol;24(2):299-304, 2017 Feb.
[Is] ISSN:1553-4669
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:STUDY OBJECTIVE: To analyze the reproductive outcome of hysteroscopic adhesiolysis and assess the diagnostic accuracy of transvaginal ultrasonography (TVS) in infertile women resulting from intrauterine adhesions (IUAs). DESIGN: Retrospective, cross-sectional study (Canadian Task Force classification II-2). SETTING: University tertiary referral center. PATIENTS: Three hundred and fifty-seven patients with mild, moderate, and severe IUAs who underwent hysteroscopic adhesiolysis between January 2012 and December 2015. INTERVENTIONS: Hysteroscopic adhesiolysis in the outpatient analgesic setting for infertility and IUAs. MEASUREMENTS AND MAIN RESULTS: Among the 357 patients (135 with mild IUAs, 116 with moderate IUAs, and 106 with severe IUAs) who underwent hysteroscopic adhesiolysis, 334 (93.6%) experienced a completely restored uterine cavity. The reproductive outcomes of 332 women (93%) were followed for an average duration of 27 ± 9 months, and the overall conception rate after hysteroscopic adhesiolysis was 48.2%, which decreased with increased IUA severity (mild, 60.7%; moderate, 53.4%; severe, 25%). The mean time to conception following hysteroscopic adhesiolysis was 9.7 ± 3.7 months. The miscarriage rate was 9.4%, and the live birth rate was no lower than 85.6%. Eleven patients (7.9%) had postpartum hemorrhage, including 6 (4.3%) due to adherent placenta and 3 (2.1%) due to placenta accreta. CONCLUSION: Hysteroscopic adhesiolysis is a feasible and effective way to improve fertility in patients with Asherman's syndrome.
[Mh] Termos MeSH primário: Ginatresia
Histeroscopia
Infertilidade Feminina
Saúde Reprodutiva/estatística & dados numéricos
[Mh] Termos MeSH secundário: Adulto
China/epidemiologia
Estudos Transversais
Dissecação/efeitos adversos
Dissecação/métodos
Feminino
Fertilização/fisiologia
Ginatresia/diagnóstico
Ginatresia/epidemiologia
Ginatresia/etiologia
Ginatresia/cirurgia
Seres Humanos
Histeroscopia/efeitos adversos
Histeroscopia/métodos
Infertilidade Feminina/diagnóstico
Infertilidade Feminina/epidemiologia
Infertilidade Feminina/etiologia
Avaliação de Processos e Resultados (Cuidados de Saúde)
Estudos Retrospectivos
Índice de Gravidade de Doença
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


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[PMID]:27751746
[Au] Autor:Yazbeck C
[Ad] Endereço:Collège de Médecine, 3, avenue Victoria, 75004 Paris, France; Hôpital Foch, 40, rue Worth, 92150 Suresnes, France; Clinique Pierre-Cherest, 5, rue P.-Cherest, 92200 Neuilly-Sur-Seine, France. Electronic address: chadiyazbeck@yahoo.fr.
[Ti] Título:[Autologus stem cells and endometrial therapy: Hope or illusion?]
[Ti] Título:Cellules souches autologues en thérapie endométriale : espoir ou illusion ?.
[So] Source:Gynecol Obstet Fertil;44(11):616-617, 2016 Nov.
[Is] ISSN:1769-6682
[Cp] País de publicação:France
[La] Idioma:fre
[Mh] Termos MeSH primário: Endométrio
Doenças dos Genitais Femininos/cirurgia
Transplante de Células-Tronco
[Mh] Termos MeSH secundário: Feminino
Ginatresia/cirurgia
Seres Humanos
Transplante Autólogo
[Pt] Tipo de publicação:EDITORIAL
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161019
[St] Status:MEDLINE


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[PMID]:27267872
[Au] Autor:Korkmazer E; Tekin B; Solak N
[Ad] Endereço:Bursa Sevket Yilmaz Research and Training Hospital, Department of Gynecology and Obstetrics, Bursa, Turkey. Electronic address: ekorkmazer@yahoo.com.
[Ti] Título:Ultrasound guidance during hysteroscopic myomectomy in G1 and G2 Submucous Myomas: for a safer one step surgery.
[So] Source:Eur J Obstet Gynecol Reprod Biol;203:108-11, 2016 Aug.
[Is] ISSN:1872-7654
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Hysteroscopic myomectomy is the gold standard method for treatment of submucous fibroids. Hysteroscopic myomectomy techniques for removal of submucous fibroids still have controversies. In this study we aimed to describe usefulness of ultrasound guidance in hysteroscopic myomectomy for a safe and effective removal of submucous G1 and G2 fibroids. STUDY DESIGN: This is a multicentre study. 64 symptomatic patients with submucous fibroid underwent ultrasound guided hysteroscopic myomectomy. First we excised intrauterine dome of fibroid until reaching the level of cavity wall by the method of resectoscopic (electrosurgical resection using a loop electrode) slicing. Next remnant intramural node was squeezed by uterine contractions induced. After we had excised the intrauterine dome of fibroid by slicing method, we formed cavitation for the intramural part (newly raised myoma dome). The cavity was filled with distension solution and we evaluated the margins of the uterus and the margins of the myoma by sonographically. Then the myoma was excised under ultrasonographic guidance by transabdominal probe. We obtained a regular uterine cavity. RESULTS: Mean operation time was 42±7min. Mean Mannitol volume was 4.3±1.7l and the mean intraoperational fluid deficit was 500ml. Not in any case uterine perforation was occurred. All fibroids removed totally. In 8 (19%) cases intrauterine synechiae detected and all these synechiaes were incised by a scissor during hysteroscopy. CONCLUSION: There is still no single technique proven to be unequivocally superior to the others for treating fibroids with intramural development (G1-G2). Ultrasound guided hysteroscopy seems to be an effective and safe method for resection of G1 and G2 fibroids.
[Mh] Termos MeSH primário: Endossonografia/efeitos adversos
Complicações Intraoperatórias/prevenção & controle
Leiomiomatose/diagnóstico por imagem
Miométrio/diagnóstico por imagem
Miomectomia Uterina/efeitos adversos
Neoplasias Uterinas/diagnóstico por imagem
Perfuração Uterina/prevenção & controle
[Mh] Termos MeSH secundário: Adulto
Procedimentos Cirúrgicos Ambulatórios/efeitos adversos
Feminino
Seguimentos
Ginatresia/epidemiologia
Ginatresia/etiologia
Ginatresia/cirurgia
Seres Humanos
Complicações Intraoperatórias/epidemiologia
Complicações Intraoperatórias/etiologia
Leiomiomatose/cirurgia
Meia-Idade
Miométrio/cirurgia
Duração da Cirurgia
Projetos Piloto
Complicações Pós-Operatórias/epidemiologia
Complicações Pós-Operatórias/etiologia
Complicações Pós-Operatórias/cirurgia
Estudos Prospectivos
Risco
Turquia/epidemiologia
Neoplasias Uterinas/cirurgia
Perfuração Uterina/epidemiologia
Perfuração Uterina/etiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; MULTICENTER STUDY
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170413
[Lr] Data última revisão:
170413
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE


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[PMID]:27172642
[Au] Autor:Bae JY; Cha HH; Seong WJ
[Ti] Título:Fetal Spinal Deformity Caused by Uterine Synechiae Mimicking Severe Scoliosis: A Case Report.
[So] Source:J Reprod Med;61(3-4):171-4, 2016 Mar-Apr.
[Is] ISSN:0024-7758
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: A severely deformed vertebra is a matter of concern, particularly when it develops before 24 weeks of gestation, which may lead to compromised pulmonary function and neurological development. CASE: A 39-year-old, nulliparous woman presented at 19 weeks of gestation. Her uterus was snowman shaped due to uterine synechiae, and the fetus was confined in the upper section, where amniotic fluid was scanty. The fetal spine was flexed at the upper thoracic level at an angle greater than 90°, with the head flexed and touching the right shoulder throughout pregnancy. Cesarean section was performed at 29+3 weeks of gestation due to preterm labor. A radiograph acquired immediately postpartum showed only a mild degree of spinal flexion, and during the course of hospitalization for respiratory support the infant's spine straightened completely. The infant was discharged without any complications. CONCLUSION: Here, we report an unusual case of severe fetal spinal deformity observed in early fetal life, and the subsequent positive outcome. We therefore advise caution, following a careful evaluation and consultation, before arriving at a decision of termination.
[Mh] Termos MeSH primário: Ginatresia/complicações
Complicações na Gravidez
Escoliose
Coluna Vertebral/anormalidades
[Mh] Termos MeSH secundário: Adulto
Líquido Amniótico
Cesárea
Diagnóstico Diferencial
Feminino
Idade Gestacional
Seres Humanos
Recém-Nascido
Recém-Nascido Prematuro
Imagem por Ressonância Magnética
Trabalho de Parto Prematuro
Gravidez
Diagnóstico Pré-Natal
Radiografia
Coluna Vertebral/diagnóstico por imagem
Coluna Vertebral/embriologia
Ultrassonografia Pré-Natal
Útero
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1605
[Cu] Atualização por classe:161126
[Lr] Data última revisão:
161126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160514
[St] Status:MEDLINE


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[PMID]:27129970
[Au] Autor:Sanad AS; Aboulfotouh ME
[Ad] Endereço:Department of Obstetrics and Gynecology, Faculty of Medicine, Minia Maternity University Hospital, Minia University, Cournish El-Nile Street, Minia, Egypt. asasanad@hotmail.com.
[Ti] Título:Hysteroscopic adhesiolysis: efficacy and safety.
[So] Source:Arch Gynecol Obstet;294(2):411-6, 2016 Aug.
[Is] ISSN:1432-0711
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To evaluate the efficacy and safety of hysteroscopic adhesiolysis in patients with intrauterine adhesions (IUAs). SETTING: Minia Maternity University Hospital, Egypt. DESIGN: Prospective cohort study. PATIENTS: This study included 61 patients presented with infertility (primary or secondary) or recurrent pregnancy losses caused by IUAs. INTERVENTION(S): The adhesions were divided by semi-rigid scissors introduced under direct vision through hysteroscopy. Three months later, second-look hysteroscopy was performed. PRIMARY OUTCOME PARAMETERS: Primary outcome parameters were reproductive parameters (pregnancy rate, duration of pregnancies, life births rate, time lag between the intervention and diagnosis of pregnancy). SECONDARY OUTCOME PARAMETERS: Secondary outcome parameters were the changes in post-operative menstrual pattern, number and duration of intervention and type of intra- and post-operative complications. RESULT(S): Pregnancy rate changed from 18 to 65.5 %, while live birth rate improved from 14.7 to 36 %. The mean time until the first conception was 10.2 months (range 2-60 months) after the operation. There was significant negative correlation between the degree of IUAs and the improvement in reproductive performance. Hysteroscopic adhesiolysis significantly improved menstrual pattern in 60.7 % of patients complaining of hypomenorrhea or amenorrhea. (p = 0.0017). The average operative time was 29 ± 10.2 (10-52) min and the hospital stay was 12.5 ± 2.1 (9-24) h. Uterine perforation occurred on 3 (4.9 %), and cervical laceration occurred in one case (1.6 %). CONCLUSION(S): Hysteroscopic adhesiolysis of IUAs is safe and effective in terms of reproductive outcome. The outcome is significantly affected by degree of intrauterine adhesions rather than the main complaint before the procedure.
[Mh] Termos MeSH primário: Ginatresia/cirurgia
Histeroscopia/métodos
Infertilidade Feminina/cirurgia
Aderências Teciduais/cirurgia
Doenças Uterinas/cirurgia
[Mh] Termos MeSH secundário: Adulto
Egito
Feminino
Fertilização/fisiologia
Seres Humanos
Histeroscopia/efeitos adversos
Infertilidade Feminina/etiologia
Menstruação
Avaliação de Processos e Resultados (Cuidados de Saúde)
Gravidez
Resultado da Gravidez
Taxa de Gravidez
Estudos Prospectivos
Aderências Teciduais/etiologia
Doenças Uterinas/complicações
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170809
[Lr] Data última revisão:
170809
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160501
[St] Status:MEDLINE
[do] DOI:10.1007/s00404-016-4107-9


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[PMID]:27005892
[Au] Autor:Santamaria X; Cabanillas S; Cervelló I; Arbona C; Raga F; Ferro J; Palmero J; Remohí J; Pellicer A; Simón C
[Ad] Endereço:FundaciÅ“n Instituto Valenciano de Infertilidad (FIVI), Department of Obstetrics & Gynecology, School of Medicine, Valencia University and Instituto Universitario IVI/INCLIVA, Valencia, Spain Instituto Valenciano Infertilidad (IVI) Barcelona, Barcelona, Spain.
[Ti] Título:Autologous cell therapy with CD133+ bone marrow-derived stem cells for refractory Asherman's syndrome and endometrial atrophy: a pilot cohort study.
[So] Source:Hum Reprod;31(5):1087-96, 2016 May.
[Is] ISSN:1460-2350
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:STUDY QUESTION: Could cell therapy using autologous peripheral blood CD133+ bone marrow-derived stem cells (BMDSCs) offer a safe and efficient therapeutic approach for patients with refractory Asherman's syndrome (AS) and/or endometrial atrophy (EA) and a wish to conceive? SUMMARY ANSWER: In the first 3 months, autologous cell therapy, using CD133+ BMDSCs in conjunction with hormonal replacement therapy, increased the volume and duration of menses as well as the thickness and angiogenesis processes of the endometrium while decreasing intrauterine adhesion scores. WHAT IS KNOWN ALREADY: AS is characterized by the presence of intrauterine adhesions and EA prevents the endometrium from growing thicker than 5 mm, resulting in menstruation disorders and infertility. Many therapies have been attempted for these conditions, but none have proved effective. STUDY DESIGN, SIZE, DURATION: This was a prospective, experimental, non-controlled study. There were 18 patients aged 30-45 years with refractory AS or EA were recruited, and 16 of these completed the study. Medical history, physical examination, endometrial thickness, intrauterine adhesion score and neoangiogenesis were assessed before and 3 and 6 months after cell therapy. PARTICIPANTS/MATERIALS, SETTING, METHODS: After the initial hysteroscopic diagnosis, BMDSC mobilization was performed by granulocyte-CSF injection, then CD133+ cells were isolated through peripheral blood aphaeresis to obtain a mean of 124.39 million cells (range 42-236), which were immediately delivered into the spiral arterioles by catheterization. Subsequently, endometrial treatment after stem cell therapy was assessed in terms of restoration of menses, endometrial thickness (by vaginal ultrasound), adhesion score (by hysteroscopy), neoangiogenesis and ongoing pregnancy rate. The study was conducted at Hospital Clínico Universitario of Valencia and IVI Valencia (Spain). MAIN RESULTS AND THE ROLE OF CHANCE: All 11 AS patients exhibited an improved uterine cavity 2 months after stem cell therapy. Endometrial thickness increased from an average of 4.3 mm (range 2.7-5) to 6.7 mm (range 3.1-12) ( ITALIC! P = 0.004). Similarly, four of the five EA patients experienced an improved endometrial cavity, and endometrial thickness increased from 4.2 mm (range 2.7-5) to 5.7 mm (range 5-12) ( ITALIC! P = 0.03). The beneficial effects of the cell therapy increased the mature vessel density and the duration and intensity of menses in the first 3 months, with a return to the initial levels 6 months after the treatment. Three patients became pregnant spontaneously, resulting in one baby boy born, one ongoing pregnancy and a miscarriage. Furthermore, seven pregnancies were obtained after fourteen embryo transfers, resulting in three biochemical pregnancies, one miscarriage, one ectopic pregnancy, one baby born and one ongoing pregnancy. LIMITATIONS, REASONS FOR CAUTION: Limitations of this pilot study include the small sample size and the lack of control group. WIDER IMPLICATIONS OF THE FINDINGS: This novel autologous cell therapy is a promising therapeutic option for patients with these incurable pathologies and a wish to conceive. STUDY FUNDING/COMPETING INTERESTS: This study was funded by the Spanish Ministry of Science and Innovation (SAF 2012-31017, Principal Investigator C.S.), Spanish Ministry of Health (EC11-299, Principal Investigator C.S.) and Regional Valencian Ministry of Education (PROMETEOII/2013/018, Principal Investigator C.S.). Four authors (X.S., I.C., A.P. and C.S.) are co-inventors of the patent resulting from this work (Application number: 62/013,121). S.C., C.A., F.R., J.F., J.P. and J.R. have no conflict of interest in relation to this work. TRIAL REGISTRATION NUMBER: This study was registered with ClinicalTrials.gov (NCT02144987).
[Mh] Termos MeSH primário: Antígeno AC133/metabolismo
Transfusão de Sangue Autóloga
Terapia Baseada em Transplante de Células e Tecidos/métodos
Ginatresia/terapia
Transplante de Células-Tronco Hematopoéticas
Transplante Autólogo
[Mh] Termos MeSH secundário: Adulto
Atrofia/terapia
Estudos de Coortes
Endométrio/patologia
Feminino
Células-Tronco Hematopoéticas/metabolismo
Seres Humanos
Meia-Idade
Projetos Piloto
Estudos Prospectivos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (AC133 Antigen); 0 (PROM1 protein, human)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170107
[Lr] Data última revisão:
170107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160324
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE
[do] DOI:10.1093/humrep/dew042


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Fotocópia
[PMID]:26982384
[Au] Autor:Liu AZ; Zhao HG; Gao Y; Liu M; Guo BZ
[Ad] Endereço:a Department of Gynecology and Obstetrics , Woman and Infant Hospital of Zhengzhou , Zhengzhou , Henan , P.R. China.
[Ti] Título:Effectiveness of estrogen treatment before transcervical resection of adhesions on moderate and severe uterine adhesion patients.
[So] Source:Gynecol Endocrinol;32(9):737-740, 2016 Sep.
[Is] ISSN:1473-0766
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Intrauterine adhesion (IUA), also known as Asherman's syndrome, is a common disease for among women. The extent of adhesion and pre-surgery hormone therapy greatly affects the function of uterine cavity. This current study investigates the association of different doses of estrogen before transcervical resection of adhesions (TCRA) surgery and clinical outcome in serious IUA. About 120 newly diagnostic serious IUA patients who underwent TCRA were randomly divided into three study groups: Estradiol valerate (progynova) 3 or 9 mg per diet before surgery and the control group. Follow-up hysteroscopy checkups were taken in 1- and 3-month post-operation. The effective power of 9 mg group was significantly higher than other groups. The 9 mg group achieved the best menstrual recovery rate in all study groups compared with the other two groups in 6 months post-operatively (p < 0.05). Our results confirmed estradiol valerateas an alternative effective drug for the prevention of IUAs before and after hysteroscopic surgery.
[Mh] Termos MeSH primário: Estradiol/análogos & derivados
Estrogênios/farmacologia
Ginatresia/prevenção & controle
Ginatresia/cirurgia
Procedimentos Cirúrgicos em Ginecologia/métodos
Avaliação de Resultados (Cuidados de Saúde)
[Mh] Termos MeSH secundário: Adulto
Estradiol/administração & dosagem
Estradiol/farmacologia
Estrogênios/administração & dosagem
Feminino
Seres Humanos
Estudos Retrospectivos
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Estrogens); 4TI98Z838E (Estradiol); OKG364O896 (estradiol valerate)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170510
[Lr] Data última revisão:
170510
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160317
[St] Status:MEDLINE



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