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  1 / 3115 MEDLINE  
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[PMID]:29302023
[Au] Autor:Bernardo MH; Tiyyagura SR
[Ad] Endereço:Department of Internal Medicine, St. Joseph's Regional Medical Center, Paterson, NJ, USA.
[Ti] Título:A Case of Type I and II Brugada Phenocopy Unmasked in a Patient with Normal Baseline Electrocardiogram (ECG).
[So] Source:Am J Case Rep;19:21-24, 2018 Jan 05.
[Is] ISSN:1941-5923
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND Brugada pattern on electrocardiogram (ECG) is seen when there are at least 2 mm J-point elevation and 1 mm ST-segment elevation in two or more of the right precordial leads, with right bundle-branch block (RBBB)-like morphology. Elevation of a coved-type shape in leads V1 and V2 is consistent with type I Brugada pattern, whereas elevation of a saddle-back configuration distinguishes type II Brugada. If accompanied by life-threatening arrhythmias or sudden cardiac death, Brugada syndrome (BrS) is diagnosed. The presence of Brugada ECG pattern in absence of the syndrome has come to be known as Brugada phenocopy (BrP). CASE REPORT We introduce a case of both Brugada type I and II patterns unmasked in a 28-year-old female with fever secondary to mastitis. Though fever-induced BrP is a universally known phenomenon, the presentation of both type I and II patterns presenting in a patient during a single hospitalization makes this case unique from others. The patient was brought to the emergency department after experiencing a syncopal episode that appeared classically vasovagal in nature. Once her fever resolved, her baseline ECG showed no abnormalities. CONCLUSIONS Though Brugada ECG pattern may be very alarming, especially after syncope, appropriate management in the case of a fever-induced event would consist of observation with cardiac monitoring, immediate treatment of fever with antipyretics, and antibiotics for suspected infection. Close follow-up by a cardiologist as an outpatient is imperative to further ascertain if the patient is at high risk of life-threatening arrhythmias, significant for BrS.
[Mh] Termos MeSH primário: Síndrome de Brugada/diagnóstico
Eletrocardiografia
[Mh] Termos MeSH secundário: Adulto
Síndrome de Brugada/etiologia
Diagnóstico Diferencial
Emergências
Feminino
Febre/etiologia
Seres Humanos
Mastite/complicações
Fatores de Risco
[Pt] Tipo de publicação:CASE REPORTS; JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180106
[St] Status:MEDLINE


  2 / 3115 MEDLINE  
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[PMID]:29240317
[Au] Autor:Meretoja T; Ihalainen H; Leidenius M
[Ti] Título:Inflammations of the mammary gland.
[So] Source:Duodecim;133(9):855-61, 2017.
[Is] ISSN:0012-7183
[Cp] País de publicação:Finland
[La] Idioma:eng
[Ab] Resumo:Breast inflammation, i.e. mastitis is an entity with a variable etiology and severity, typically affecting women of fertile age. In most cases, anamnesis reveals the etiology of the disease, bacterial puerperal mastitis associated with breastfeeding being the most common. Periductal mastitis is a breast inflammation typically associated with smoking in women of 40 to 50 years of age, localized to the region of the areola. Granulomatous mastitis and other rare breast inflammations usually require specialized care. Regardless of etiology, treatment of acute inflammation depends on the clinical picture and, with the exception of puerperal mastitis, emergency ultrasound imaging is often necessary in order to detect an abscess and drain it. In all cases of mastitis, the possibility of underlying malignancy as well as inflammatory breast cancer should be kept in mind. If specialized care is required, breast inflammation is treated in a breast, general or plastic surgery unit.
[Mh] Termos MeSH primário: Mastite/diagnóstico
Mastite/etiologia
Mastite/terapia
[Mh] Termos MeSH secundário: Adulto
Diagnóstico Diferencial
Feminino
Seres Humanos
Meia-Idade
Fatores de Risco
Ultrassonografia Mamária
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180115
[Lr] Data última revisão:
180115
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171215
[St] Status:MEDLINE


  3 / 3115 MEDLINE  
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[PMID]:28926595
[Au] Autor:Catozzi C; Sanchez Bonastre A; Francino O; Lecchi C; De Carlo E; Vecchio D; Martucciello A; Fraulo P; Bronzo V; Cuscó A; D'Andreano S; Ceciliani F
[Ad] Endereço:Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Via Celoria 10, Milano, Italy.
[Ti] Título:The microbiota of water buffalo milk during mastitis.
[So] Source:PLoS One;12(9):e0184710, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to define the microbiota of water buffalo milk during sub-clinical and clinical mastitis, as compared to healthy status, by using high-throughput sequencing of the 16S rRNA gene. A total of 137 quarter samples were included in the experimental design: 27 samples derived from healthy, culture negative quarters, with a Somatic Cell Count (SCC) of less than 200,000 cells/ml; 27 samples from quarters with clinical mastitis; 83 samples were collected from quarters with subclinical mastitis, with a SCC number greater of 200,000 cells/ml and/or culture positive for udder pathogens, without clinical signs of mastitis. Bacterial DNA was purified and the 16S rRNA genes were individually amplified and sequenced. Significant differences were found in milk samples from healthy quarters and those with sub-clinical and clinical mastitis. The microbiota diversity of milk from healthy quarters was richer as compared to samples with sub-clinical mastitis, whose microbiota diversity was in turn richer as compared to those from clinical mastitis. The core microbiota of water buffalo milk, defined as the asset of microorganisms shared by all healthy milk samples, includes 15 genera, namely Micrococcus, Propionibacterium, 5-7N15, Solibacillus, Staphylococcus, Aerococcus, Facklamia, Trichococcus, Turicibacter, 02d06, SMB53, Clostridium, Acinetobacter, Psychrobacter and Pseudomonas. Only two genera (Acinetobacter and Pseudomonas) were present in all the samples from sub-clinical mastitis, and no genus was shared across all in clinical mastitis milk samples. The presence of mastitis was found to be related to the change in the relative abundance of genera, such as Psychrobacter, whose relative abundance decreased from 16.26% in the milk samples from healthy quarters to 3.2% in clinical mastitis. Other genera, such as SMB53 and Solibacillus, were decreased as well. Discriminant analysis presents the evidence that the microbial community of healthy and clinical mastitis could be discriminated on the background of their microbiota profiles.
[Mh] Termos MeSH primário: Mastite/diagnóstico
Microbiota
Leite/microbiologia
[Mh] Termos MeSH secundário: Animais
Bactérias/genética
Bactérias/isolamento & purificação
Búfalos
DNA Bacteriano/genética
DNA Bacteriano/metabolismo
Análise Discriminante
Feminino
Sequenciamento de Nucleotídeos em Larga Escala
Mastite/microbiologia
RNA Ribossômico 16S/química
RNA Ribossômico 16S/genética
RNA Ribossômico 16S/metabolismo
Análise de Sequência de DNA
Células-Tronco/citologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170920
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184710


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[PMID]:28873396
[Au] Autor:Camperio C; Armas F; Biasibetti E; Frassanito P; Giovannelli C; Spuria L; D'Agostino C; Tait S; Capucchio MT; Marianelli C
[Ad] Endereço:Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy.
[Ti] Título:A mouse mastitis model to study the effects of the intramammary infusion of a food-grade Lactococcus lactis strain.
[So] Source:PLoS One;12(9):e0184218, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lactococcus lactis is one of the most important microorganisms in the dairy industry and has "generally recognized as safe" (GRAS) status. L. lactis belongs to the group of lactic acid bacteria (LAB) and is encountered in a wide range of environments. Recently, the use of the intramammary infusion of a live culture of LAB has been investigated as a new antibiotic alternative for treating mastitis in dairy ruminants. Controversial results are described in literature regarding its efficacy and safety. In this study we conducted in-depth investigation of the mammary gland immune response induced by intramammary inoculum of a live culture of L. lactis LMG 7930 using the mouse mastitis model. Overnight cultures either of L. lactis (≈ 107 CFU) or of the mastitis pathogens Staphylococcus chromogenes (≈ 105 CFU) or S. aureus (≈ 102 CFU/ml) were injected into the mouse inguinal glands. A double injection, consisting of S. chromogenes first and then L. lactis, was also investigated. Bacterial recovery from the gland and inflammatory cell infiltration were assessed. L. lactis-treated and control glands were analysed for proinflammatory cytokine production. Microbiological results showed that L. lactis was able to survive in the mammary gland 24 h post infection, as were the mastitis pathogens S. chromogenes and S. aureus. L. lactis reduced S. chromogenes survival in the glands and increased its own survival ability by coexisting with the pathogen. Histology showed that L. lactis-treated glands presented variable histological features, ranging from undamaged tissue with no inflammatory cell infiltrate to severe PMN infiltrate with focal areas of tissue damage. S. aureus-treated glands showed the most severe histological grade of inflammation despite the fact that the inoculum size was the smallest. In contrast, most S. chromogenes-treated glands showed normal structures with no infiltration or lesions. Significant increases in IL-1ß and TNF-α levels were also found in L. lactis-inoculated glands. The above findings seem to suggest that food-grade L. lactis at a high-inoculum dose such as an overnight culture may elicit a suppurative inflammatory response in the mammary gland, thus becoming a potential mastitis-causing pathogen. Because of the unpredictable potential of L. lactis in acting as a potential mastitis pathogen, this organism cannot be considered a safe treatment for bovine mastitis.
[Mh] Termos MeSH primário: Lactococcus lactis/fisiologia
Glândulas Mamárias Animais/microbiologia
Glândulas Mamárias Animais/patologia
Mastite/microbiologia
[Mh] Termos MeSH secundário: Animais
Citocinas/biossíntese
Modelos Animais de Doenças
Feminino
Mediadores da Inflamação/metabolismo
Mastite/patologia
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Inflammation Mediators)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170906
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184218


  5 / 3115 MEDLINE  
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[PMID]:28573222
[Au] Autor:Balpinar N; Okmen G
[Ad] Endereço:Department of Biology, Faculty of Arts and Science, Mehmet Akif Ersoy University, Burdur 15030, Turkey.
[Ti] Título:THE BIOLOGICAL ACTIVITIES OF ., AN ENDEMIC SPECIES TO TURKEY.
[So] Source:Afr J Tradit Complement Altern Med;14(2):60-64, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: is the direct reason of mastitis. Mastitis is a disease characterized by pathological changes in mammary glands as well as physical, chemical, bacteriological changes in milk. This disease causes loses in milk yield and quality. In recent years, it is reported that mastitis pathogens have developed a resistance to antibiotics as a natural consequence of widespread use of it. Today's researches are focused on discovering and using new antibiotics against bacteria. The aim of this paper is to examine the antibacterial properties of (an endemic species to Turkey), and its other biological activities. MATERIALS AND METHODS: All of the extracts were tested by disc diffusion assay in order to screen antibacterial activity. MIC values were evaluated as antibacterial activities of plant extracts. The non-enzymatic antioxidative activities including DPPH radical scavenging effects were studied . RESULTS AND CONCLUSIONS: Results shown that the extracts had strong antibacterial effects on three bacteria 17, and CNS-37) and the range of inhibition zone was 4-6mm. This three bacteria screened the lowest sensitivity to 65000 µg /mL concentration. Besides, the extracts were tested for non-enzymatic antioxidant activities. As a result, the methanol extract of the flower displayed a strong antioxidant activity. The various extracts of have different antibacterial and antioxidant properties.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antioxidantes/farmacologia
Boraginaceae
Extratos Vegetais/farmacologia
Staphylococcus aureus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Compostos de Bifenilo/metabolismo
Flores
Mastite/microbiologia
Testes de Sensibilidade Microbiana
Picratos/metabolismo
Folhas de Planta
Caules de Planta
Staphylococcus aureus/crescimento & desenvolvimento
Turquia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Picrates); 0 (Plant Extracts); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i2.7


  6 / 3115 MEDLINE  
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[PMID]:28542575
[Au] Autor:Yu GM; Kubota H; Okita M; Maeda T
[Ad] Endereço:Department of Bioresource Science, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
[Ti] Título:The anti-inflammatory and antioxidant effects of melatonin on LPS-stimulated bovine mammary epithelial cells.
[So] Source:PLoS One;12(5):e0178525, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mastitis is the most prevalent disease in dairy cattle worldwide and not only causes huge economic losses in the dairy industry but also threatens public health. To evaluate the therapeutic potential of melatonin in mastitis, we examined the ability of melatonin to protect bovine mammary epithelial cells (bMECs) from the harmful effects of lipopolysaccharide (LPS). We found that melatonin inhibited the LPS-binding protein-CD14-TLR4 signaling pathway in bMECs, which had opposing effects on pro-inflammatory and anti-inflammatory mediators. Melatonin decreased LPS-induced expression of pro-inflammatory cytokines, chemokines, and positive acute-phase proteins (APPs), including tumor necrosis factor-α, interleukin (IL)-1ß, IL-6, granulocyte-monocyte colony-stimulating factor, chemokine CC motif ligand (CCL)2, CCL5, serum amyloid A, haptoglobin, C-reactive protein, ceruloplasmin, and α-1 antitrypsin, and increased expression of the anti-inflammatory cytokine IL-1Ra and the negative APP fibrinogen. In addition, melatonin increased dityrosine levels but suppressed nitrite levels by upregulating the expression of Nrf2 and heme oxygenase-1 in the Nrf2 antioxidant defense pathway. Finally, melatonin administration increased the viability of LPS-stimulated bMECs. These results suggest that melatonin protects bMECs from LPS-induced inflammatory and oxidant stress damage and provide evidence that melatonin might have therapeutic utility in mastitis.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Células Epiteliais/efeitos dos fármacos
Lipopolissacarídeos/farmacologia
Melatonina/farmacologia
[Mh] Termos MeSH secundário: Proteínas da Fase Aguda/metabolismo
Animais
Proteína C-Reativa/metabolismo
Proteínas de Transporte/metabolismo
Bovinos
Doenças dos Bovinos/tratamento farmacológico
Doenças dos Bovinos/metabolismo
Fatores Estimuladores de Colônias/metabolismo
Células Epiteliais/metabolismo
Feminino
Heme Oxigenase-1/metabolismo
Proteína Antagonista do Receptor de Interleucina 1/metabolismo
Interleucina-1beta/metabolismo
Interleucina-6/metabolismo
Receptores de Lipopolissacarídeos/metabolismo
Glândulas Mamárias Animais
Mastite/tratamento farmacológico
Mastite/metabolismo
Glicoproteínas de Membrana/metabolismo
Transdução de Sinais/efeitos dos fármacos
Receptor 4 Toll-Like/metabolismo
Fator de Necrose Tumoral alfa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acute-Phase Proteins); 0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Carrier Proteins); 0 (Colony-Stimulating Factors); 0 (Interleukin 1 Receptor Antagonist Protein); 0 (Interleukin-1beta); 0 (Interleukin-6); 0 (Lipopolysaccharide Receptors); 0 (Lipopolysaccharides); 0 (Membrane Glycoproteins); 0 (Toll-Like Receptor 4); 0 (Tumor Necrosis Factor-alpha); 0 (lipopolysaccharide-binding protein); 9007-41-4 (C-Reactive Protein); EC 1.14.14.18 (Heme Oxygenase-1); JL5DK93RCL (Melatonin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178525


  7 / 3115 MEDLINE  
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[PMID]:28418794
[Au] Autor:Mediano P; Fernández L; Jiménez E; Arroyo R; Espinosa-Martos I; Rodríguez JM; Marín M
[Ad] Endereço:1 Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, Madrid, Spain.
[Ti] Título:Microbial Diversity in Milk of Women With Mastitis: Potential Role of Coagulase-Negative Staphylococci, Viridans Group Streptococci, and Corynebacteria.
[So] Source:J Hum Lact;33(2):309-318, 2017 May.
[Is] ISSN:1552-5732
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Lactational mastitis constitutes a significant cause of premature weaning. However, its etiology, linked to the presence of pathogenic microorganisms, has been scarcely reported. Research aim: The aim of this study was to describe the microbial diversity in milk samples from women suffering from lactational mastitis and to identify more accurately a collection of isolates belonging to coagulase-negative staphylococci, streptococci, and coryneform bacteria. METHODS: This is a cross-sectional descriptive one-group study. A total of 5,009 isolates from 1,849 mastitis milk samples was identified by culture, biochemical, and/or molecular methods at the species or genus level. A more precise identification of a collection of 211 isolates was carried out by 16S rRNA gene sequencing. RESULTS: Mean total bacterial count in milk samples was 4.11 log colony-forming units/ml, 95% confidence interval [4.08, 4.15]. Staphylococcus epidermidis was the most common species being isolated from 91.56% of the samples, whereas Staphylococcus aureus was detected in 29.74%. Streptococci and corynebacteria constituted the second (70.20%) and third (16.60%) most prevalent bacterial groups, respectively, found in this study. In contrast, Candida spp. was present in only 0.54% of the samples. Sequencing of the 16S rRNA gene revealed a high diversity of bacterial species among identified isolates. CONCLUSION: Many coagulase-negative staphylococci, viridans group streptococci, and corynebacteria, usually dismissed as contaminant bacteria, may play an important role as etiologic agents of mastitis. Proper diagnosis of mastitis should be established after performing microbiological testing of milk based on standardized procedures. A reliable analysis must identify the mastitis-causing pathogen(s) at the species level and its(their) concentration(s).
[Mh] Termos MeSH primário: Mastite/microbiologia
Fenômenos Microbiológicos
Leite Humano/microbiologia
[Mh] Termos MeSH secundário: Adulto
Aleitamento Materno/efeitos adversos
Corynebacterium/patogenicidade
Estudos Transversais
Feminino
Seres Humanos
Lactação/metabolismo
RNA Ribossômico 16S/análise
Espanha
Infecções Estafilocócicas/complicações
Infecções Estafilocócicas/genética
Staphylococcus aureus/patogenicidade
Staphylococcus epidermidis/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:N
[Da] Data de entrada para processamento:170419
[St] Status:MEDLINE
[do] DOI:10.1177/0890334417692968


  8 / 3115 MEDLINE  
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[PMID]:28413048
[Au] Autor:Vasiu I; Dabrowski R; Martinez-Subiela S; Ceron JJ; Wdowiak A; Pop RA; Brudasca FG; Pastor J; Tvarijonaviciute A
[Ad] Endereço:Department and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Agricultural Science and Veterinary Medicine, 3-5 Calea Manastur St, 400372 Cluj-Napoca, Romania. Electronic address: iosif.vasiu@usamvcluj.ro.
[Ti] Título:Milk C-reactive protein in canine mastitis.
[So] Source:Vet Immunol Immunopathol;186:41-44, 2017 Apr.
[Is] ISSN:1873-2534
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Presence of mastitis in lactating bitches can become life threatening for both the bitch and pups. The aim of the present study was to evaluate a possible utility of C-reactive protein (CRP) in both milk and serum for canine mastitis diagnosis. Our study showed that milk CRP levels ranged between 0.1 and 4.9µg/mL and from 0.3 to 40.0µg/mL in healthy and diseased bitches (P<0.01), respectively, while serum CRP levels ranged between 2.0 and 8.6µg/mL and between 0.3 and 162.3µg/mL in healthy and diseased bitches (P<0.01), respectively. Milk and serum CRP levels were higher in both clinical and subclinical mastitis when compared with healthy controls (P<0.05 in all cases). However, no significant differences were recorded in CRP concentrations between clinical and subclinical cases. Based on these results, it could be concluded that serum and milk CRP could be useful in order to diagnose canine mastitis.
[Mh] Termos MeSH primário: Proteína C-Reativa/análise
Doenças do Cão/diagnóstico
Cães
Mastite/veterinária
Leite/química
[Mh] Termos MeSH secundário: Animais
Doenças do Cão/sangue
Feminino
Mastite/sangue
Mastite/diagnóstico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9007-41-4 (C-Reactive Protein)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170418
[St] Status:MEDLINE


  9 / 3115 MEDLINE  
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[PMID]:28351714
[Au] Autor:Moliva MV; Cerioli F; Reinoso EB
[Ad] Endereço:Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36 Km 601, X5804ZAB Río Cuarto, Córdoba, Argentina.
[Ti] Título:Evaluation of environmental and nutritional factors and sua gene on in vitro biofilm formation of Streptococcus uberis isolates.
[So] Source:Microb Pathog;107:144-148, 2017 Jun.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The pathogenesis of Streptococcus uberis is attributed to a combination of extracellular factors and properties such as adherence and biofilm formation. The aim of this work was to evaluate the influence of different factors, additives and bovine milk compounds on S. uberis biofilm formation, as the presence of the sua gene by PCR. Additionally, extracellular DNA and the effect of DNaseI were evaluated in the biofilms yielded. Optimal biofilm development was observed when the pH was adjusted to 7.0 and 37 °C. Additives as glucose and lactose reduced biofilm formation as bovine milk compounds tested. PCR assay showed that not all the isolates yielded sua gene. Extrachromosomal ADN was found in cell-free supernatants, suggesting that DNA released spontaneously to the medium. The results contribute to a better understanding of the factors involved in biofilm production of this important pathogen associated with mastitis in order to promote the design of new therapeutic approaches.
[Mh] Termos MeSH primário: Aderência Bacteriana/efeitos dos fármacos
Aderência Bacteriana/genética
Proteínas de Bactérias/genética
Biofilmes/efeitos dos fármacos
Biofilmes/crescimento & desenvolvimento
Streptococcus/genética
[Mh] Termos MeSH secundário: Animais
Argentina
Caseínas/farmacologia
Bovinos
Meios de Cultura/química
DNA Bacteriano/genética
Desoxirribonuclease I/farmacologia
Feminino
Genes Bacterianos/genética
Glucose/farmacologia
Concentração de Íons de Hidrogênio
Lactose/farmacologia
Mastite/microbiologia
Mastite Bovina/microbiologia
Leite/química
Leite/microbiologia
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
Soroalbumina Bovina/farmacologia
Infecções Estreptocócicas/microbiologia
Streptococcus/efeitos dos fármacos
Streptococcus/isolamento & purificação
Streptococcus/patogenicidade
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Caseins); 0 (Culture Media); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S); 27432CM55Q (Serum Albumin, Bovine); 65072-00-6 (casein hydrolysate); EC 3.1.21.1 (Deoxyribonuclease I); IY9XDZ35W2 (Glucose); J2B2A4N98G (Lactose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170330
[St] Status:MEDLINE


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[PMID]:28318584
[Au] Autor:Veissier I; Mialon MM; Sloth KH
[Ad] Endereço:INRA, UMR1213 Herbivores, F-63122 Saint-Genès-Champanelle, France; VetAgro Sup, Clermont Université, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France. Electronic address: isabelle.veissier@clermont.inra.fr.
[Ti] Título:Short communication: Early modification of the circadian organization of cow activity in relation to disease or estrus.
[So] Source:J Dairy Sci;100(5):3969-3974, 2017 May.
[Is] ISSN:1525-3198
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Biological rhythms are an essential regulator of life. There is evidence that circadian rhythm of activity is disrupted under chronic stress in animals and humans, and it may also be less marked during diseases. Here we investigated whether a detectable circadian rhythm of activity exists in dairy cows in commercial settings using a real-time positioning system. We used CowView (GEA Farm Technologies) to regularly record the individual positions of 350 cows in a Danish dairy farm over 5 mo and to infer the cows' activity (resting, feeding, in alley). We ran a factorial correspondence analysis on the cows' activities and used the first component of this analysis to express the variations in activity. On this axis, the activities obtained the following weights: resting = -0.15; in alleys = +0.12; feeding = +0.34. By applying these weights to the proportions of time each cow spent on each of the 3 activities, we were able to chart a circadian rhythm of activity. We found that average level of activity of a cow on a given day and its variations during that day varied with specific states (i.e., estrus, lameness, mastitis). More specifically, circadian variations in activity appeared to be particularly sensitive and to vary 1 to 2 d before the farmer detected a disorder. These findings offer promising avenues for further research to design models to predict physiological or pathological states of cows from real-time positioning data.
[Mh] Termos MeSH primário: Ritmo Circadiano
Estro
Leite
[Mh] Termos MeSH secundário: Animais
Bovinos
Doenças dos Bovinos
Indústria de Laticínios
Feminino
Marcha
Seres Humanos
Mastite
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE



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